divergent subfamily of APPLE domains
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 502 |
| AciI | CCGC | 1 cut(s) | 215 |
| AclWI | GGATC | 2 cut(s) | 62, 791 |
| AcsI | RAATTY | 4 cut(s) | 16, 676, 743, 843 |
| AfaI | GTAC | 4 cut(s) | 260, 448, 468, 566 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 77 |
| AgeI | ACCGGT | 1 cut(s) | 273 |
| AgsI | TTSAA | 2 cut(s) | 179, 457 |
| AjnI | CCWGG | 2 cut(s) | 599, 633 |
| AluBI | AGCT | 5 cut(s) | 7, 140, 536, 543, 835 |
| AluI | AGCT | 5 cut(s) | 7, 140, 536, 543, 835 |
| Alw21I | GWGCWC | 1 cut(s) | 62 |
| Alw26I | GTCTC | 2 cut(s) | 49, 518 |
| AlwI | GGATC | 2 cut(s) | 62, 791 |
| ApeKI | GCWGC | 3 cut(s) | 540, 705, 782 |
| ApoI | RAATTY | 4 cut(s) | 16, 676, 743, 843 |
| AsiGI | ACCGGT | 1 cut(s) | 273 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 655 |
| AsuHPI | GGTGA | 2 cut(s) | 739, 944 |
| AsuNHI | GCTAGC | 1 cut(s) | 835 |
| Bbv12I | GWGCWC | 1 cut(s) | 62 |
| BbvI | GCAGC | 3 cut(s) | 552, 692, 769 |
| BccI | CCATC | 2 cut(s) | 91, 545 |
| BceAI | ACGGC | 2 cut(s) | 187, 791 |
| BciT130I | CCWGG | 2 cut(s) | 601, 635 |
| BciVI | GTATCC | 1 cut(s) | 94 |
| BcoDI | GTCTC | 2 cut(s) | 49, 518 |
| BfaI | CTAG | 4 cut(s) | 153, 417, 794, 836 |
| BfmI | CTRYAG | 2 cut(s) | 546, 801 |
| BfuI | GTATCC | 1 cut(s) | 94 |
| BisI | GCNGC | 3 cut(s) | 541, 706, 783 |
| BlsI | GCNGC | 3 cut(s) | 542, 707, 784 |
| Bme1390I | CCNGG | 2 cut(s) | 601, 635 |
| BmiI | GGNNCC | 3 cut(s) | 357, 725, 951 |
| BmrFI | CCNGG | 2 cut(s) | 601, 635 |
| BmsI | GCATC | 1 cut(s) | 794 |
| BmtI | GCTAGC | 1 cut(s) | 839 |
| BpuEI | CTTGAG | 3 cut(s) | 60, 608, 744 |
| BsaAI | YACGTR | 1 cut(s) | 347 |
| BsaJI | CCNNGG | 1 cut(s) | 634 |
| BsaWI | WCCGGW | 2 cut(s) | 273, 952 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 934, 964 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 77 |
| Bse118I | RCCGGY | 1 cut(s) | 273 |
| Bse1I | ACTGG | 3 cut(s) | 31, 190, 209 |
| Bse3DI | GCAATG | 1 cut(s) | 312 |
| BseBI | CCWGG | 2 cut(s) | 601, 635 |
| BseDI | CCNNGG | 1 cut(s) | 634 |
| BseGI | GGATG | 3 cut(s) | 102, 247, 518 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 77 |
| BseMI | GCAATG | 1 cut(s) | 312 |
| BseMII | CTCAG | 2 cut(s) | 386, 934 |
| BseNI | ACTGG | 3 cut(s) | 31, 190, 209 |
| BseRI | GAGGAG | 2 cut(s) | 70, 257 |
| BseXI | GCAGC | 3 cut(s) | 552, 692, 769 |
| BshTI | ACCGGT | 1 cut(s) | 273 |
| BsiHKAI | GWGCWC | 1 cut(s) | 62 |
| BsiSI | CCGG | 3 cut(s) | 71, 274, 953 |
| BslFI | GGGAC | 1 cut(s) | 175 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 77 |
| BsmAI | GTCTC | 2 cut(s) | 49, 518 |
| BsmFI | GGGAC | 1 cut(s) | 175 |
| BsmI | GAATGC | 1 cut(s) | 882 |
| Bsp1286I | GDGCHC | 1 cut(s) | 62 |
| Bsp1407I | TGTACA | 1 cut(s) | 446 |
| Bsp143I | GATC | 4 cut(s) | 67, 379, 796, 849 |
| BspACI | CCGC | 1 cut(s) | 215 |
| BspCNI | CTCAG | 2 cut(s) | 385, 933 |
| BspLI | GGNNCC | 3 cut(s) | 357, 725, 951 |
| BspOI | GCTAGC | 1 cut(s) | 839 |
| BspPI | GGATC | 2 cut(s) | 62, 791 |
| BsrDI | GCAATG | 1 cut(s) | 312 |
| BsrFI | RCCGGY | 1 cut(s) | 273 |
| BsrGI | TGTACA | 1 cut(s) | 446 |
| BsrI | ACTGG | 3 cut(s) | 31, 190, 209 |
| BssAI | RCCGGY | 1 cut(s) | 273 |
| BssECI | CCNNGG | 1 cut(s) | 634 |
| BssMI | GATC | 4 cut(s) | 67, 379, 796, 849 |
| Bst2UI | CCWGG | 2 cut(s) | 601, 635 |
| Bst4CI | ACNGT | 3 cut(s) | 321, 569, 656 |
| Bst6I | CTCTTC | 1 cut(s) | 905 |
| BstAUI | TGTACA | 1 cut(s) | 446 |
| BstBAI | YACGTR | 1 cut(s) | 347 |
| BstC8I | GCNNGC | 4 cut(s) | 538, 710, 780, 837 |
| BstDEI | CTNAG | 4 cut(s) | 141, 237, 372, 920 |
| BstF5I | GGATG | 3 cut(s) | 102, 247, 518 |
| BstKTI | GATC | 4 cut(s) | 70, 382, 799, 852 |
| BstMAI | GTCTC | 2 cut(s) | 49, 518 |
| BstMBI | GATC | 4 cut(s) | 67, 379, 796, 849 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 822 |
| BstNI | CCWGG | 2 cut(s) | 601, 635 |
| BstNSI | RCATGY | 1 cut(s) | 453 |
| BstSCI | CCNGG | 2 cut(s) | 599, 633 |
| BstSFI | CTRYAG | 2 cut(s) | 546, 801 |
| BstV1I | GCAGC | 3 cut(s) | 552, 692, 769 |
| BstX2I | RGATCY | 2 cut(s) | 67, 796 |
| BstYI | RGATCY | 2 cut(s) | 67, 796 |
| BsuI | GTATCC | 1 cut(s) | 94 |
| BtsCI | GGATG | 3 cut(s) | 102, 247, 518 |
| BtsIMutI | CAGTG | 2 cut(s) | 326, 389 |
| Cac8I | GCNNGC | 4 cut(s) | 538, 710, 780, 837 |
| Cfr10I | RCCGGY | 1 cut(s) | 273 |
| Csp6I | GTAC | 4 cut(s) | 259, 447, 467, 565 |
| CspAI | ACCGGT | 1 cut(s) | 273 |
| CviAII | CATG | 5 cut(s) | 107, 165, 450, 616, 660 |
| CviQI | GTAC | 4 cut(s) | 259, 447, 467, 565 |
| DdeI | CTNAG | 4 cut(s) | 141, 237, 372, 920 |
| DpnI | GATC | 4 cut(s) | 69, 381, 798, 851 |
| DpnII | GATC | 4 cut(s) | 67, 379, 796, 849 |
| DraI | TTTAAA | 1 cut(s) | 577 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 502 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 502 |
| Eam1104I | CTCTTC | 1 cut(s) | 905 |
| EarI | CTCTTC | 1 cut(s) | 905 |
| Eco32I | GATATC | 1 cut(s) | 191 |
| EcoRI | GAATTC | 2 cut(s) | 743, 843 |
| EcoRII | CCWGG | 2 cut(s) | 599, 633 |
| EcoRV | GATATC | 1 cut(s) | 191 |
| FaeI | CATG | 5 cut(s) | 110, 168, 453, 619, 663 |
| FaqI | GGGAC | 1 cut(s) | 175 |
| FatI | CATG | 5 cut(s) | 106, 164, 449, 615, 659 |
| FauNDI | CATATG | 2 cut(s) | 307, 670 |
| Fnu4HI | GCNGC | 3 cut(s) | 541, 706, 783 |
| FokI | GGATG | 3 cut(s) | 109, 254, 525 |
| Fsp4HI | GCNGC | 3 cut(s) | 541, 706, 783 |
| FspBI | CTAG | 4 cut(s) | 153, 417, 794, 836 |
| GluI | GCNGC | 3 cut(s) | 541, 706, 783 |
| HapII | CCGG | 3 cut(s) | 71, 274, 953 |
| Hin1II | CATG | 5 cut(s) | 110, 168, 453, 619, 663 |
| HincII | GTYRAC | 1 cut(s) | 234 |
| HindII | GTYRAC | 1 cut(s) | 234 |
| HindIII | AAGCTT | 1 cut(s) | 5 |
| HinfI | GANTC | 2 cut(s) | 526, 755 |
| HpaII | CCGG | 3 cut(s) | 71, 274, 953 |
| HphI | GGTGA | 2 cut(s) | 739, 944 |
| Hpy166II | GTNNAC | 2 cut(s) | 234, 261 |
| Hpy188I | TCNGA | 5 cut(s) | 480, 754, 790, 849, 923 |
| Hpy188III | TCNNGA | 2 cut(s) | 521, 761 |
| Hpy8I | GTNNAC | 2 cut(s) | 234, 261 |
| HpyAV | CCTTC | 2 cut(s) | 127, 686 |
| HpyCH4III | ACNGT | 3 cut(s) | 321, 569, 656 |
| HpyCH4IV | ACGT | 1 cut(s) | 346 |
| HpyCH4V | TGCA | 6 cut(s) | 287, 317, 540, 668, 674, 785 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 822 |
| HpyF3I | CTNAG | 4 cut(s) | 141, 237, 372, 920 |
| HpySE526I | ACGT | 1 cut(s) | 346 |
| Hsp92II | CATG | 5 cut(s) | 110, 168, 453, 619, 663 |
| Kzo9I | GATC | 4 cut(s) | 67, 379, 796, 849 |
| LmnI | GCTCC | 3 cut(s) | 57, 137, 955 |
| Lsp1109I | GCAGC | 3 cut(s) | 552, 692, 769 |
| LweI | GCATC | 1 cut(s) | 794 |
| MaeI | CTAG | 4 cut(s) | 153, 417, 794, 836 |
| MaeII | ACGT | 1 cut(s) | 346 |
| MaeIII | GTNAC | 3 cut(s) | 22, 431, 569 |
| MalI | GATC | 4 cut(s) | 69, 381, 798, 851 |
| MboI | GATC | 4 cut(s) | 67, 379, 796, 849 |
| MboII | GAAGA | 4 cut(s) | 77, 585, 588, 922 |
| MflI | RGATCY | 2 cut(s) | 67, 796 |
| MhlI | GDGCHC | 1 cut(s) | 62 |
| MluCI | AATT | 8 cut(s) | 16, 179, 499, 578, 676, 743, 843, 968 |
| MlyI | GAGTC | 1 cut(s) | 520 |
| MnlI | CCTC | 7 cut(s) | 48, 278, 632, 737, 760, 776, 906 |
| MroXI | GAANNNNTTC | 1 cut(s) | 655 |
| MseI | TTAA | 4 cut(s) | 182, 299, 576, 960 |
| MspI | CCGG | 3 cut(s) | 71, 274, 953 |
| MspR9I | CCNGG | 2 cut(s) | 601, 635 |
| Mva1269I | GAATGC | 1 cut(s) | 882 |
| MvaI | CCWGG | 2 cut(s) | 601, 635 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 822 |
| NdeI | CATATG | 2 cut(s) | 307, 670 |
| NdeII | GATC | 4 cut(s) | 67, 379, 796, 849 |
| NheI | GCTAGC | 1 cut(s) | 835 |
| NlaIII | CATG | 5 cut(s) | 110, 168, 453, 619, 663 |
| NlaIV | GGNNCC | 3 cut(s) | 357, 725, 951 |
| NspI | RCATGY | 1 cut(s) | 453 |
| PctI | GAATGC | 1 cut(s) | 882 |
| PdmI | GAANNNNTTC | 1 cut(s) | 655 |
| PfeI | GAWTC | 1 cut(s) | 755 |
| PinAI | ACCGGT | 1 cut(s) | 273 |
| PkrI | GCNGC | 3 cut(s) | 542, 707, 784 |
| PleI | GAGTC | 1 cut(s) | 520 |
| PpsI | GAGTC | 1 cut(s) | 520 |
| Ppu21I | YACGTR | 1 cut(s) | 347 |
| Psp6I | CCWGG | 2 cut(s) | 599, 633 |
| PspGI | CCWGG | 2 cut(s) | 599, 633 |
| PspN4I | GGNNCC | 3 cut(s) | 357, 725, 951 |
| PsuI | RGATCY | 2 cut(s) | 67, 796 |
| RsaI | GTAC | 4 cut(s) | 260, 448, 468, 566 |
| RsaNI | GTAC | 4 cut(s) | 259, 447, 467, 565 |
| SaqAI | TTAA | 4 cut(s) | 182, 299, 576, 960 |
| SatI | GCNGC | 3 cut(s) | 541, 706, 783 |
| Sau3AI | GATC | 4 cut(s) | 67, 379, 796, 849 |
| SchI | GAGTC | 1 cut(s) | 520 |
| ScrFI | CCNGG | 2 cut(s) | 601, 635 |
| SduI | GDGCHC | 1 cut(s) | 62 |
| SfaNI | GCATC | 1 cut(s) | 794 |
| SfcI | CTRYAG | 2 cut(s) | 546, 801 |
| SmlI | CTYRAG | 3 cut(s) | 75, 623, 759 |
| SmoI | CTYRAG | 3 cut(s) | 75, 623, 759 |
| Sse9I | AATT | 8 cut(s) | 16, 179, 499, 578, 676, 743, 843, 968 |
| SsiI | CCGC | 1 cut(s) | 215 |
| SspMI | CTAG | 4 cut(s) | 153, 417, 794, 836 |
| StyD4I | CCNGG | 2 cut(s) | 599, 633 |
| TaaI | ACNGT | 3 cut(s) | 321, 569, 656 |
| TaiI | ACGT | 1 cut(s) | 349 |
| TaqI | TCGA | 1 cut(s) | 494 |
| TasI | AATT | 8 cut(s) | 16, 179, 499, 578, 676, 743, 843, 968 |
| TatI | WGTACW | 3 cut(s) | 258, 446, 564 |
| TfiI | GAWTC | 1 cut(s) | 755 |
| Tru1I | TTAA | 4 cut(s) | 182, 299, 576, 960 |
| Tru9I | TTAA | 4 cut(s) | 182, 299, 576, 960 |
| TscAI | CASTG | 2 cut(s) | 326, 389 |
| TseI | GCWGC | 3 cut(s) | 540, 705, 782 |
| TspDTI | ATGAA | 6 cut(s) | 17, 146, 258, 352, 648, 883 |
| TspGWI | ACGGA | 2 cut(s) | 109, 922 |
| TspRI | CASTG | 2 cut(s) | 326, 389 |
| XapI | RAATTY | 4 cut(s) | 16, 676, 743, 843 |
| XceI | RCATGY | 1 cut(s) | 453 |
| XmnI | GAANNNNTTC | 1 cut(s) | 655 |
| XspI | CTAG | 4 cut(s) | 153, 417, 794, 836 |