Rh5BG186300

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000207
Physical Location & Seq
Reverse (-)
20474588 .. 20475800
1213 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG186300.1

Sequence Viewer

Length: 861 bp
ATGGATCCTCATGGTTTACCTCAATTTTTTGAAGTTCAGGGAGCTAAGATACTGACTAGAGCAGGATCATGGAACGGCCTTCAATTGACTGGATATCAAAGGAGGCCAAATCCAATATCTGAGTTTGTATTTGTGTTGAATGAGACAGAAATCTATTATGAGTACACACTCCTCAACCGGTCTACGTTCTCTAGATATGTAATGAATCCATATGGCACTACACAGTGGTTAACATGGATAGACTACACAAATAGTTGGGAGCCTTTCTTTGCAAGCCAAGCAGATCAGTGTGAAATTTACGCTTTTTGTGGGGCTAATGCTAATTGTAATGTCAATGAAGCCCCCATGTGTGCATGCTTGAAAGGATTTGTACCTAAATCTCCACAAAAGTGGAACTCTTCACGTTGGTCTGATGGATGTGTTCGAAGGACTCCATTAGTTTGCAGTGATAGAGATGACTTCTTAATATATAGTAGATTTAAATTGCCAGACACATCTTCTTCCTGGTATGACAAGAGCATGAACCTCAAGGAATGCAAGGAATTATGTTTGAGAAACTGCTCGTGTACGGCTTATGCAAATTTAGATGTCAAGGAAGGGGGAAGTGGCTGCTTGCTTTGGTTTGGAAACCTCACCGACATAAAAGAATTTACCTCTGATTCTCAAGACCTCTATATACGAATAGCTTCTTCAGATACAGATGCTATTGGGAAGAAGAGCAAGTCCAACAAGAGTAGGCAAGCTGGAATTGTAATCAGCTCTGCTCTTCTTGTAGTGGGAATGCTAATACTTGGATTCCTCTTGTATAAACGGAACAAGAAACTCAGAAATCAAGGTAAACTTCTCACAGATGGACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

286

Amino Acids

32.47

Weight (kDa)

8.53

Isoelectric Point (pI)

44.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S_locus_glycop PF00954 20 - 127 6.7e-24 S-locus glycoprotein domain
PAN_2 PF08276 148 - 214 2.8e-22 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27300
fragaria_vesca FvH4_3g15930 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g21350 FvH4_3g21400 FvH4_3g21400 FvH4_6g12332
malus_domestica MD02G1167100.v1.1 MD02G1167200.v1.1 MD03G1185600.v1.1 MD05G1213900.v1.1 MD05G1214100.v1.1 MD05G1214200.v1.1 MD05G1214700.v1.1 MD05G1216300.v1.1 MD05G1216800.v1.1 MD05G1217300.v1.1 MD05G1218000.v1.1 MD11G1231100.v1.1 MD11G1231200.v1.1 MD11G1231400.v1.1 MD11G1231500.v1.1 MD11G1232400.v1.1 MD11G1232500.v1.1
prunus_persica Prupe.4G142300_v2.0.a1 Prupe.4G142400_v2.0.a1 Prupe.4G142500_v2.0.a1 Prupe.4G142600_v2.0.a1 Prupe.4G142800_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195100_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195500_v2.0.a1 Prupe.4G195700_v2.0.a1 Prupe.4G195900_v2.0.a1
pyrus_communis pycom03g16540 pycom05g19920 pycom11g20460 pycom11g20480 pycom11g20490 pycom11g20500 pycom11g20530
rosa_chinensis RchiOBHm_Chr2g0139311 RchiOBHm_Chr2g0139351 RchiOBHm_Chr5g0026801 RchiOBHm_Chr5g0026851 RchiOBHm_Chr5g0026891 RchiOBHm_Chr5g0026911 RchiOBHm_Chr5g0036571 RchiOBHm_Chr5g0036591 RchiOBHm_Chr5g0036721 RchiOBHm_Chr5g0036741 RchiOBHm_Chr7g0222981
rosa_laevigata RLG00000002006 RLG00000019807 RLG00000032964 RLG00000032968 RLG00000032969 RLG00000033706 RLG00000033708 RLG00000033712 RLG00000033716
rosa_multiflora Rmu_co8256651.1_g000001 Rmu_co8279377.1_g000001 Rmu_co8429919.1_g000001 Rmu_co8490103.1_g000001 Rmu_sc0000774.1_g000002 Rmu_sc0002525.1_g000012 Rmu_sc0004223.1_g000008 Rmu_sc0004390.1_g000002 Rmu_sc0006059.1_g000014 Rmu_sc0006059.1_g000024 Rmu_sc0006514.1_g000020 Rmu_sc0014333.1_g000002 Rmu_ssc0000172.1_g000018 Rmu_ssc0000172.1_g000029
rosa_roxburghii Rroxscaffold_1G00044230 Rroxscaffold_1G00044260 Rroxscaffold_1G00044320 Rroxscaffold_1G00044340 Rroxscaffold_1G00052560 Rroxscaffold_1G00052570 Rroxscaffold_1G00052660 Rroxscaffold_1G00052720 Rroxscaffold_2G00105450 Rroxscaffold_2G00105460
rosa_rugosa Rorug02G0356900 Rorug02G0357000 Rorug02G0357100 Rorug02G0357200 Rorug02G0357300 Rorug02G0357400 Rorug02G0357500 Rorug02G0357600 Rorug05G0095400 Rorug05G0095500 Rorug05G0095500 Rorug05G0095600 Rorug05G0095700 Rorug05G0095800 Rorug05G0157700 Rorug05G0157800
rosa_samantha Rh2AG406800 Rh2AG406900 Rh2BG417300 Rh2CG392900 Rh2DG426200 Rh5AG189300 Rh5AG249400 Rh5AG250100 Rh5BG186300 Rh5BG186900 Rh5BG187000 Rh5BG249500 Rh5BG250600 Rh5BG250900 Rh5BG251500 Rh5BG251800 Rh5CG205500 Rh5CG206300 Rh5CG206700 Rh5CG283000 Rh5CG283300 Rh5CG283500 Rh5DG188500 Rh5DG188700 Rh5DG258700 Rh5DG258900 Rh5DG259600 Rh5DG259900 Rh7CG375800 Rh7DG352500
rosa_wichuraiana Rw2G032960 Rw2G032970 Rw2G033220 Rw2G033230 Rw5G017130 Rw5G017230 Rw5G022940 Rw5G022960 Rw5G022980 Rw7G030420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 182
AclWI GGATC 2 cut(s) 12, 73
AcsI RAATTY 3 cut(s) 294, 580, 647
AcuI CTGAAG 1 cut(s) 675
AfaI GTAC 3 cut(s) 164, 372, 568
AgeI ACCGGT 1 cut(s) 177
AgsI TTSAA 4 cut(s) 32, 83, 139, 361
AjnI CCWGG 1 cut(s) 503
AleI CACNNNNGTG 1 cut(s) 388
AluBI AGCT 4 cut(s) 44, 686, 743, 759
AluI AGCT 4 cut(s) 44, 686, 743, 759
Alw26I GTCTC 1 cut(s) 137
AlwI GGATC 2 cut(s) 12, 73
AoxI GGCC 2 cut(s) 76, 104
ApeKI GCWGC 1 cut(s) 609
ApoI RAATTY 3 cut(s) 294, 580, 647
AsiGI ACCGGT 1 cut(s) 177
Asp700I GAANNNNTTC 1 cut(s) 685
AsuHPI GGTGA 1 cut(s) 625
AsuII TTCGAA 1 cut(s) 424
BamHI GGATCC 1 cut(s) 4
BauI CACGAG 1 cut(s) 562
BbvI GCAGC 1 cut(s) 596
BccI CCATC 2 cut(s) 407, 845
BceAI ACGGC 2 cut(s) 91, 585
BciT130I CCWGG 1 cut(s) 505
BcoDI GTCTC 1 cut(s) 137
BfaI CTAG 2 cut(s) 57, 192
BisI GCNGC 1 cut(s) 610
BlsI GCNGC 1 cut(s) 611
Bme1390I CCNGG 1 cut(s) 505
BmiI GGNNCC 2 cut(s) 6, 261
BmrFI CCNGG 1 cut(s) 505
BmsI GCATC 1 cut(s) 691
Bpu14I TTCGAA 1 cut(s) 424
BpuEI CTTGAG 2 cut(s) 512, 648
BsaWI WCCGGW 1 cut(s) 177
Bse118I RCCGGY 1 cut(s) 177
Bse1I ACTGG 1 cut(s) 94
BseBI CCWGG 1 cut(s) 505
BseGI GGATG 1 cut(s) 422
BseMII CTCAG 2 cut(s) 111, 838
BseNI ACTGG 1 cut(s) 94
BseRI GAGGAG 1 cut(s) 161
BseXI GCAGC 1 cut(s) 596
BshFI GGCC 2 cut(s) 78, 106
BshTI ACCGGT 1 cut(s) 177
BsiSI CCGG 1 cut(s) 178
BsmAI GTCTC 1 cut(s) 137
BsmI GAATGC 2 cut(s) 539, 786
BsnI GGCC 2 cut(s) 78, 106
Bsp119I TTCGAA 1 cut(s) 424
Bsp143I GATC 3 cut(s) 4, 65, 283
BspANI GGCC 2 cut(s) 78, 106
BspCNI CTCAG 2 cut(s) 112, 837
BspLI GGNNCC 2 cut(s) 6, 261
BspPI GGATC 2 cut(s) 12, 73
BspQI GCTCTTC 2 cut(s) 710, 771
BspT104I TTCGAA 1 cut(s) 424
BsrFI RCCGGY 1 cut(s) 177
BsrI ACTGG 1 cut(s) 94
BssAI RCCGGY 1 cut(s) 177
BssMI GATC 3 cut(s) 4, 65, 283
BssSI CACGAG 1 cut(s) 562
Bst2BI CACGAG 1 cut(s) 562
Bst2UI CCWGG 1 cut(s) 505
Bst4CI ACNGT 1 cut(s) 225
Bst6I CTCTTC 3 cut(s) 403, 710, 771
BstBI TTCGAA 1 cut(s) 424
BstC8I GCNNGC 4 cut(s) 274, 355, 614, 741
BstDEI CTNAG 3 cut(s) 45, 120, 824
BstF5I GGATG 1 cut(s) 422
BstKTI GATC 3 cut(s) 7, 68, 286
BstMAI GTCTC 1 cut(s) 137
BstMBI GATC 3 cut(s) 4, 65, 283
BstMWI GCNNNNNNNGC 1 cut(s) 278
BstNI CCWGG 1 cut(s) 505
BstNSI RCATGY 1 cut(s) 357
BstSCI CCNGG 1 cut(s) 503
BstV1I GCAGC 1 cut(s) 596
BstX2I RGATCY 1 cut(s) 4
BstXI CCANNNNNNTGG 1 cut(s) 390
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 2 cut(s) 78, 106
BtsCI GGATG 1 cut(s) 422
BtsI GCAGTG 1 cut(s) 451
BtsIMutI CAGTG 3 cut(s) 230, 293, 451
Cac8I GCNNGC 4 cut(s) 274, 355, 614, 741
Cfr10I RCCGGY 1 cut(s) 177
Csp6I GTAC 3 cut(s) 163, 371, 567
CspAI ACCGGT 1 cut(s) 177
CviAII CATG 6 cut(s) 11, 69, 234, 346, 354, 520
CviQI GTAC 3 cut(s) 163, 371, 567
DdeI CTNAG 3 cut(s) 45, 120, 824
DpnI GATC 3 cut(s) 6, 67, 285
DpnII GATC 3 cut(s) 4, 65, 283
DraI TTTAAA 1 cut(s) 481
Eam1104I CTCTTC 3 cut(s) 403, 710, 771
EarI CTCTTC 3 cut(s) 403, 710, 771
Eco32I GATATC 1 cut(s) 95
Eco57I CTGAAG 1 cut(s) 675
EcoRII CCWGG 1 cut(s) 503
EcoRV GATATC 1 cut(s) 95
FaeI CATG 6 cut(s) 14, 72, 237, 349, 357, 523
FalI AAGNNNNNCTT 2 cut(s) 825, 857
FatI CATG 6 cut(s) 10, 68, 233, 345, 353, 519
FauNDI CATATG 1 cut(s) 211
FblI GTMKAC 1 cut(s) 182
Fnu4HI GCNGC 1 cut(s) 610
FokI GGATG 1 cut(s) 429
Fsp4HI GCNGC 1 cut(s) 610
FspBI CTAG 2 cut(s) 57, 192
GluI GCNGC 1 cut(s) 610
HaeIII GGCC 2 cut(s) 78, 106
HapII CCGG 1 cut(s) 178
Hin1II CATG 6 cut(s) 14, 72, 237, 349, 357, 523
HincII GTYRAC 1 cut(s) 231
HindII GTYRAC 1 cut(s) 231
HinfI GANTC 5 cut(s) 205, 430, 659, 795, 855
HpaI GTTAAC 1 cut(s) 231
HpaII CCGG 1 cut(s) 178
HphI GGTGA 1 cut(s) 625
Hpy166II GTNNAC 6 cut(s) 17, 165, 183, 231, 567, 839
Hpy188I TCNGA 6 cut(s) 121, 412, 658, 694, 827, 860
Hpy188III TCNNGA 2 cut(s) 192, 665
Hpy8I GTNNAC 6 cut(s) 17, 165, 183, 231, 567, 839
HpyAV CCTTC 3 cut(s) 89, 420, 590
HpyCH4III ACNGT 1 cut(s) 225
HpyCH4IV ACGT 2 cut(s) 185, 403
HpyCH4V TGCA 5 cut(s) 272, 353, 444, 537, 578
HpyF10VI GCNNNNNNNGC 1 cut(s) 278
HpyF3I CTNAG 3 cut(s) 45, 120, 824
HpySE526I ACGT 2 cut(s) 185, 403
Hsp92II CATG 6 cut(s) 14, 72, 237, 349, 357, 523
KspAI GTTAAC 1 cut(s) 231
Kzo9I GATC 3 cut(s) 4, 65, 283
LguI GCTCTTC 2 cut(s) 710, 771
LmnI GCTCC 2 cut(s) 41, 259
LpnPI CCDG 8 cut(s) 23, 48, 75, 191, 490, 501, 517, 729
Lsp1109I GCAGC 1 cut(s) 596
LweI GCATC 1 cut(s) 691
MaeI CTAG 2 cut(s) 57, 192
MaeII ACGT 2 cut(s) 185, 403
MalI GATC 3 cut(s) 6, 67, 285
MboI GATC 3 cut(s) 4, 65, 283
MboII GAAGA 7 cut(s) 390, 489, 492, 681, 724, 727, 758
MfeI CAATTG 1 cut(s) 83
MflI RGATCY 1 cut(s) 4
MluCI AATT 9 cut(s) 23, 83, 294, 322, 482, 542, 580, 647, 747
MlyI GAGTC 2 cut(s) 424, 849
MmeI TCCRAC 1 cut(s) 750
MnlI CCTC 9 cut(s) 18, 30, 96, 182, 536, 641, 664, 680, 809
MroXI GAANNNNTTC 1 cut(s) 685
MseI TTAA 3 cut(s) 230, 464, 480
MslI CAYNNNNRTG 1 cut(s) 388
MspI CCGG 1 cut(s) 178
MspR9I CCNGG 1 cut(s) 505
MunI CAATTG 1 cut(s) 83
Mva1269I GAATGC 2 cut(s) 539, 786
MvaI CCWGG 1 cut(s) 505
MwoI GCNNNNNNNGC 1 cut(s) 278
NdeI CATATG 1 cut(s) 211
NdeII GATC 3 cut(s) 4, 65, 283
NlaIII CATG 6 cut(s) 14, 72, 237, 349, 357, 523
NlaIV GGNNCC 2 cut(s) 6, 261
NspI RCATGY 1 cut(s) 357
NspV TTCGAA 1 cut(s) 424
OliI CACNNNNGTG 1 cut(s) 388
PaeI GCATGC 1 cut(s) 357
PciSI GCTCTTC 2 cut(s) 710, 771
PctI GAATGC 2 cut(s) 539, 786
PdmI GAANNNNTTC 1 cut(s) 685
PfeI GAWTC 3 cut(s) 205, 659, 795
PinAI ACCGGT 1 cut(s) 177
PkrI GCNGC 1 cut(s) 611
PleI GAGTC 2 cut(s) 424, 849
PpsI GAGTC 2 cut(s) 424, 849
Psp6I CCWGG 1 cut(s) 503
PspGI CCWGG 1 cut(s) 503
PspN4I GGNNCC 2 cut(s) 6, 261
PsuI RGATCY 1 cut(s) 4
RsaI GTAC 3 cut(s) 164, 372, 568
RsaNI GTAC 3 cut(s) 163, 371, 567
RseI CAYNNNNRTG 1 cut(s) 388
SapI GCTCTTC 2 cut(s) 710, 771
SaqAI TTAA 3 cut(s) 230, 464, 480
SatI GCNGC 1 cut(s) 610
Sau3AI GATC 3 cut(s) 4, 65, 283
SchI GAGTC 2 cut(s) 424, 849
ScrFI CCNGG 1 cut(s) 505
SfaNI GCATC 1 cut(s) 691
SfuI TTCGAA 1 cut(s) 424
SmiI ATTTAAAT 1 cut(s) 481
SmiMI CAYNNNNRTG 1 cut(s) 388
SmlI CTYRAG 2 cut(s) 527, 663
SmoI CTYRAG 2 cut(s) 527, 663
SphI GCATGC 1 cut(s) 357
Sse9I AATT 9 cut(s) 23, 83, 294, 322, 482, 542, 580, 647, 747
SspMI CTAG 2 cut(s) 57, 192
StyD4I CCNGG 1 cut(s) 503
SwaI ATTTAAAT 1 cut(s) 481
TaaI ACNGT 1 cut(s) 225
TaiI ACGT 2 cut(s) 188, 406
TaqI TCGA 1 cut(s) 424
TasI AATT 9 cut(s) 23, 83, 294, 322, 482, 542, 580, 647, 747
TatI WGTACW 1 cut(s) 162
TfiI GAWTC 3 cut(s) 205, 659, 795
Tru1I TTAA 3 cut(s) 230, 464, 480
Tru9I TTAA 3 cut(s) 230, 464, 480
TscAI CASTG 3 cut(s) 230, 293, 451
TseI GCWGC 1 cut(s) 609
TspDTI ATGAA 3 cut(s) 218, 351, 536
TspGWI ACGGA 1 cut(s) 826
TspRI CASTG 3 cut(s) 230, 293, 451
XapI RAATTY 3 cut(s) 294, 580, 647
XbaI TCTAGA 1 cut(s) 191
XceI RCATGY 1 cut(s) 357
XmiI GTMKAC 1 cut(s) 182
XmnI GAANNNNTTC 1 cut(s) 685
XspI CTAG 2 cut(s) 57, 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.