Prupe.4G142800_v2.0.a1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000207
Physical Location & Seq
Reverse (-)
8190297 .. 8194499
4203 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G142800.1

Sequence Viewer

Length: 2544 bp
ATGCCTTTTACTTCTAGTTTTTGGTTCTTCCATCTTCTCTGGAGAAGTCCTGTGCTAATTTTTTTTCGATACAAGTATACAATGCAAGCCTTGAGAAGCATTTTTGTGTTCTACTTCTTATTTCCCTTCTTAAGAATCTCCACTCCAACAACACTAGACACAATTACTCCAAATCGATATATTAGAGACGGTGAAAGTCTAGTTTCAGCTGGTGGAGGCTTCGAACTGGGATTCTTTAGCCCTGGTACATCAAAGGGCCGATACTTGGGAATATGGTACACTGTTGATACTGAGACAGTTGTATGGGTAGCCAACAGAGAAACACCACTTGGCGATACTTCTGGAGTTTTGACTGTCACTGACCGAGGAGTTCTAGTCCTTCTCAATAGCACAAACAGCATTGTATGGTCATCAAATTCATCAACAACTGCAGGGAATCCAGTATCACAACTCTTGGATTCAGGAAATCTTGTTGTGAAAGATGGAAATGAAACCAACCCTGTTAACTTGTTGTGGCAGAGTTTTGATTATCCTTGTGACACATTACTACCAGAAATGAAGCTTGGTTGGGACTTAGTTACTGGTTTAGAGAGGTACATCTCGTCTTGGAGGAGCAGAGACGATCCTGCTCCAGGAGAGTTTTCATTACGGATAGATCATCGTGGTTTCCCACAAGCTGTTGTTCTGAAAGGAGCTAAGAGAGTGGCTTCAGCAGGCTCATGGAACGGCCTTCATTTTACAGCATATTCATATCCTCCACAAACACGAGCAAACCCAACATTGGAGTATGAATTTGTGTTGAACAAGGATGAGATCTATTATGAGTACAGGCTCTCAAAGAGGTCCACGTTCTCAAGATATATATTGAATCCATCAGGCATTGCGCAGCGATTAACATGGGTGCACCAAACACATAGTTGGGAACTTTCCTCTACATTTCAAGCAGAGCGGTGTCAAAATTATGCTTTGTGTGGTGCATATGCTAGTTGCAAAATGAATGTCTCTCCCTTATGTGCATGCTTGAAGGGATTTGTACCAAAATCTCCAAAAGATTGGAATTCGGGATATTGGTCTGATGGGTGTGTTCGAAAGATTCCATTGGCTTGCAGCTCGGGAGATGGCTTCCTAAAGTACACTGGGGTTAAATTGCCAGACACATCTTCCACATGGTTTGATAAAAGCAAGAGCCTCAAGGAATGCAAGGGATTATGTTTGAACAACTGCTCATGTACGGCATATGCAAATTTAGATATCAGGGAGGGAGGAACTGGCTGCTTGCTTTGGTTTGGCAAGCTCATTGAAATAACGGAATTCACTTCCGGTGGTGGTCAAGACCTCTATATACGGATGGCAGCTTCAGAACTTGATCAAATTGAGAAAAAGAGCAAGTTCAACAAGAAAAAGCTACCTGGAATTCTTCTCATCAGCTCTACACTTTTTCTTTTGGGAACTTTAATAATTGGCTTGATATTGTATAGACGGAAGAAGAAACTCAGAAGTCAAGGAGTCAGAAGCATGGATTGCAGAAAAGATTGCCTTGGAGAAGACATGGGATTACCACTATTAAACTTGACCACCGTAGCTAATGCCACTAATGAGTTTTCAAGCAGTAACAAGCTGGGAGAAGGTGGTTTTGGACCTGTGTACAAGGGTACGTTGATAGAAGGGAAAGAAATTGCAGTGAAAAGGCTTTCAAAGGATTCCAGACAAGGACTGAGGGAGTTCAAAAATGAAGTTATGCTGATAGCCAGACTTCAACATCGCAATCTTGTAAAGCTTTTGGGTTGTTGCATTCAAGAAGATGAAAAAATTTTGATATATGAATTCATGACCAACAGAAGCTTGGACTTCTTTATTTTTGATCACGAGGGACAAAAGTTGCTCGACTGGGCTACATGCTTCCACATTATTGGTGGAATTGCTAGAGGGCTTCTTTATCTTCACCAAGACTCTAGATTGAGAATTATCCATAGAGATTTGAAAGCTAGCAATATCCTGCTTGATAATAATATGAATCCTAAGATTTCAGACTTCGGCCTCGCTAAAACATTTGGTAGTGATCAAAGTCGGGGTAATACAAAAAGAGTGGTTGGAACGTATGGTTACATGTCTCCCGAATATGCAGTAGATGGAATTTTCTCGATGAAATCTGATGTCTTTAGCTTTGGAGTAATACTGCTAGAGATGTTGAGTAGGCAAAAGAACAGGGGATTTTCTAACCCAGATCACCACCTTAACCTTCTTGGACATGCATGGATACTATGGATTGAAGATAAAGCATTGGAACTGATTGATAAGACATTACGTGATTCGTGCACCATATCTGAAGTGTTAAGGTGTCTTCATGTGGCGCTGTTATGTGTGCAAAGAGTACCTGAAGATAGACCAAGCATGTCATCTGTGGTTCTAATGTTGAGCAGTGATGTTACCTTGCCTCCACCAAAGCAGCCTGGTTTTTACACCGAGCGAAGTGTCCCTGAATCCCCCTTAAGTGCATGCCGGTGTTCAGCGAATACTTCCATTACAATAATAGAGGCTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

848

Amino Acids

95.24

Weight (kDa)

8.82

Isoelectric Point (pI)

47.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27300
fragaria_vesca FvH4_3g15930 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g21350 FvH4_3g21400 FvH4_3g21400 FvH4_6g12332
malus_domestica MD02G1167100.v1.1 MD02G1167200.v1.1 MD03G1185600.v1.1 MD05G1213900.v1.1 MD05G1214100.v1.1 MD05G1214200.v1.1 MD05G1214700.v1.1 MD05G1216300.v1.1 MD05G1216800.v1.1 MD05G1217300.v1.1 MD05G1218000.v1.1 MD11G1231100.v1.1 MD11G1231200.v1.1 MD11G1231400.v1.1 MD11G1231500.v1.1 MD11G1232400.v1.1 MD11G1232500.v1.1
prunus_persica Prupe.4G142300_v2.0.a1 Prupe.4G142400_v2.0.a1 Prupe.4G142500_v2.0.a1 Prupe.4G142600_v2.0.a1 Prupe.4G142800_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195100_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195500_v2.0.a1 Prupe.4G195700_v2.0.a1 Prupe.4G195900_v2.0.a1
pyrus_communis pycom03g16540 pycom05g19920 pycom11g20460 pycom11g20480 pycom11g20490 pycom11g20500 pycom11g20530
rosa_chinensis RchiOBHm_Chr2g0139311 RchiOBHm_Chr2g0139351 RchiOBHm_Chr5g0026801 RchiOBHm_Chr5g0026851 RchiOBHm_Chr5g0026891 RchiOBHm_Chr5g0026911 RchiOBHm_Chr5g0036571 RchiOBHm_Chr5g0036591 RchiOBHm_Chr5g0036721 RchiOBHm_Chr5g0036741 RchiOBHm_Chr7g0222981
rosa_laevigata RLG00000002006 RLG00000019807 RLG00000032964 RLG00000032968 RLG00000032969 RLG00000033706 RLG00000033708 RLG00000033712 RLG00000033716
rosa_multiflora Rmu_co8256651.1_g000001 Rmu_co8279377.1_g000001 Rmu_co8429919.1_g000001 Rmu_co8490103.1_g000001 Rmu_sc0000774.1_g000002 Rmu_sc0002525.1_g000012 Rmu_sc0004223.1_g000008 Rmu_sc0004390.1_g000002 Rmu_sc0006059.1_g000014 Rmu_sc0006059.1_g000024 Rmu_sc0006514.1_g000020 Rmu_sc0014333.1_g000002 Rmu_ssc0000172.1_g000018 Rmu_ssc0000172.1_g000029
rosa_roxburghii Rroxscaffold_1G00044230 Rroxscaffold_1G00044260 Rroxscaffold_1G00044320 Rroxscaffold_1G00044340 Rroxscaffold_1G00052560 Rroxscaffold_1G00052570 Rroxscaffold_1G00052660 Rroxscaffold_1G00052720 Rroxscaffold_2G00105450 Rroxscaffold_2G00105460
rosa_rugosa Rorug02G0356900 Rorug02G0357000 Rorug02G0357100 Rorug02G0357200 Rorug02G0357300 Rorug02G0357400 Rorug02G0357500 Rorug02G0357600 Rorug05G0095400 Rorug05G0095500 Rorug05G0095500 Rorug05G0095600 Rorug05G0095700 Rorug05G0095800 Rorug05G0157700 Rorug05G0157800
rosa_samantha Rh2AG406800 Rh2AG406900 Rh2BG417300 Rh2CG392900 Rh2DG426200 Rh5AG189300 Rh5AG249400 Rh5AG250100 Rh5BG186300 Rh5BG186900 Rh5BG187000 Rh5BG249500 Rh5BG250600 Rh5BG250900 Rh5BG251500 Rh5BG251800 Rh5CG205500 Rh5CG206300 Rh5CG206700 Rh5CG283000 Rh5CG283300 Rh5CG283500 Rh5DG188500 Rh5DG188700 Rh5DG258700 Rh5DG258900 Rh5DG259600 Rh5DG259900 Rh7CG375800 Rh7DG352500
rosa_wichuraiana Rw2G032960 Rw2G032970 Rw2G033220 Rw2G033230 Rw5G017130 Rw5G017230 Rw5G022940 Rw5G022960 Rw5G022980 Rw7G030420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 885
AccBSI CCGCTC 1 cut(s) 949
AccI GTMKAC 1 cut(s) 77
AciI CCGC 1 cut(s) 949
AclWI GGATC 1 cut(s) 617
AcsI RAATTY 9 cut(s) 415, 791, 1057, 1243, 1310, 1413, 1809, 1823, 2133
AcuI CTGAAG 4 cut(s) 693, 1341, 2346, 2397
AfiI CCNNNNNNNGG 3 cut(s) 265, 632, 781
AflII CTTAAG 2 cut(s) 130, 2488
AflIII ACRYGT 1 cut(s) 2106
AjnI CCWGG 4 cut(s) 241, 631, 1408, 2449
AjuI GAANNNNNNNTTGG 6 cut(s) 312, 344, 546, 578, 1617, 1649
Alw21I GWGCWC 2 cut(s) 906, 2318
Alw26I GTCTC 5 cut(s) 180, 287, 612, 1006, 2115
Alw44I GTGCAC 2 cut(s) 902, 2314
AlwI GGATC 1 cut(s) 617
Ama87I CYCGRG 1 cut(s) 1111
AoxI GGCC 3 cut(s) 256, 727, 2035
ApaLI GTGCAC 2 cut(s) 902, 2314
ApeKI GCWGC 5 cut(s) 886, 1107, 1272, 1352, 2446
ApoI RAATTY 9 cut(s) 415, 791, 1057, 1243, 1310, 1413, 1809, 1823, 2133
ArsI GACNNNNNNTTYG 2 cut(s) 984, 1016
AspLEI GCGC 2 cut(s) 886, 2353
AspS9I GGNCC 3 cut(s) 256, 843, 1637
AsuHPI GGTGA 3 cut(s) 203, 1934, 2219
AsuII TTCGAA 2 cut(s) 222, 1087
AsuNHI GCTAGC 1 cut(s) 1985
AvaI CYCGRG 1 cut(s) 1111
AvaII GGWCC 2 cut(s) 843, 1637
BaeGI GKGCMC 2 cut(s) 906, 2318
BauI CACGAG 2 cut(s) 765, 1865
BbsI GAAGAC 2 cut(s) 1551, 2333
Bbv12I GWGCWC 2 cut(s) 906, 2318
BbvI GCAGC 5 cut(s) 898, 1119, 1259, 1364, 2458
BccI CCATC 7 cut(s) 39, 476, 880, 1070, 1112, 1342, 2123
BceAI ACGGC 2 cut(s) 742, 1248
BciT130I CCWGG 4 cut(s) 243, 633, 1410, 2451
BciVI GTATCC 1 cut(s) 2250
BclI TGATCA 3 cut(s) 1366, 1861, 2059
BcoDI GTCTC 5 cut(s) 180, 287, 612, 1006, 2115
BfaI CTAG 9 cut(s) 15, 155, 200, 374, 984, 1923, 1953, 1986, 2180
BfmI CTRYAG 1 cut(s) 429
BfoI RGCGCY 1 cut(s) 2354
BfrI CTTAAG 2 cut(s) 130, 2488
BfuI GTATCC 1 cut(s) 2250
BglII AGATCT 1 cut(s) 813
BisI GCNGC 5 cut(s) 887, 1108, 1273, 1353, 2447
BlsI GCNGC 5 cut(s) 888, 1109, 1274, 1354, 2448
Bme1390I CCNGG 4 cut(s) 243, 633, 1410, 2451
Bme18I GGWCC 2 cut(s) 843, 1637
BmeT110I CYCGRG 1 cut(s) 1111
BmgT120I GGNCC 3 cut(s) 256, 843, 1637
BmrFI CCNGG 4 cut(s) 243, 633, 1410, 2451
BmrI ACTGGG 3 cut(s) 236, 1146, 1897
BmtI GCTAGC 1 cut(s) 1989
BmuI ACTGGG 3 cut(s) 236, 1146, 1897
BpiI GAAGAC 2 cut(s) 1551, 2333
BpmI CTGGAG 3 cut(s) 61, 363, 615
Bpu14I TTCGAA 2 cut(s) 222, 1087
BpuEI CTTGAG 3 cut(s) 112, 838, 1175
Bsa29I ATCGAT 1 cut(s) 175
BsaAI YACGTR 1 cut(s) 2306
BsaJI CCNNGG 3 cut(s) 241, 364, 1537
BsaWI WCCGGW 1 cut(s) 1319
BsaXI ACNNNNNCTCC 6 cut(s) 151, 181, 360, 390, 2419, 2449
Bsc4I CCNNNNNNNGG 3 cut(s) 265, 632, 781
Bse118I RCCGGY 1 cut(s) 2499
Bse1I ACTGG 6 cut(s) 231, 440, 586, 1141, 1273, 1892
Bse3DI GCAATG 1 cut(s) 879
BseBI CCWGG 4 cut(s) 243, 633, 1410, 2451
BseCI ATCGAT 1 cut(s) 175
BseDI CCNNGG 3 cut(s) 241, 364, 1537
BseGI GGATG 2 cut(s) 814, 1353
BseLI CCNNNNNNNGG 3 cut(s) 265, 632, 781
BseMI GCAATG 1 cut(s) 879
BseMII CTCAG 3 cut(s) 282, 1507, 1706
BseNI ACTGG 6 cut(s) 231, 440, 586, 1141, 1273, 1892
BseRI GAGGAG 2 cut(s) 381, 625
BseSI GKGCMC 2 cut(s) 906, 2318
BseXI GCAGC 5 cut(s) 898, 1119, 1259, 1364, 2458
BseYI CCCAGC 1 cut(s) 1618
BshFI GGCC 3 cut(s) 258, 729, 2037
BshVI ATCGAT 1 cut(s) 175
BsiHKAI GWGCWC 2 cut(s) 906, 2318
BsiHKCI CYCGRG 1 cut(s) 1111
BsiSI CCGG 2 cut(s) 1320, 2500
BslFI GGGAC 3 cut(s) 584, 1884, 2459
BslI CCNNNNNNNGG 3 cut(s) 265, 632, 781
BsmAI GTCTC 5 cut(s) 180, 287, 612, 1006, 2115
BsmBI CGTCTC 2 cut(s) 180, 612
BsmFI GGGAC 3 cut(s) 584, 1884, 2459
BsmI GAATGC 2 cut(s) 1202, 1791
BsnI GGCC 3 cut(s) 258, 729, 2037
BsoBI CYCGRG 1 cut(s) 1111
Bsp119I TTCGAA 2 cut(s) 222, 1087
Bsp1286I GDGCHC 2 cut(s) 906, 2318
Bsp1407I TGTACA 1 cut(s) 1644
Bsp143I GATC 7 cut(s) 622, 655, 813, 1366, 1861, 2059, 2224
BspACI CCGC 1 cut(s) 949
BspANI GGCC 3 cut(s) 258, 729, 2037
BspCNI CTCAG 3 cut(s) 283, 1506, 1707
BspDI ATCGAT 1 cut(s) 175
BspHI TCATGA 1 cut(s) 1827
BspMAI CTGCAG 1 cut(s) 433
BspOI GCTAGC 1 cut(s) 1989
BspPI GGATC 1 cut(s) 617
BspT104I TTCGAA 2 cut(s) 222, 1087
BspTI CTTAAG 2 cut(s) 130, 2488
BsrBI CCGCTC 1 cut(s) 949
BsrDI GCAATG 1 cut(s) 879
BsrFI RCCGGY 1 cut(s) 2499
BsrGI TGTACA 1 cut(s) 1644
BsrI ACTGG 6 cut(s) 231, 440, 586, 1141, 1273, 1892
BssAI RCCGGY 1 cut(s) 2499
BssECI CCNNGG 3 cut(s) 241, 364, 1537
BssMI GATC 7 cut(s) 622, 655, 813, 1366, 1861, 2059, 2224
BssNAI GTATAC 1 cut(s) 78
BssSI CACGAG 2 cut(s) 765, 1865
BssT1I CCWWGG 1 cut(s) 1537
Bst1107I GTATAC 1 cut(s) 78
Bst2BI CACGAG 2 cut(s) 765, 1865
Bst2UI CCWGG 4 cut(s) 243, 633, 1410, 2451
Bst4CI ACNGT 5 cut(s) 191, 283, 298, 355, 1579
BstAFI CTTAAG 2 cut(s) 130, 2488
BstAPI GCANNNNNTGC 1 cut(s) 1521
BstAUI TGTACA 1 cut(s) 1644
BstBAI YACGTR 1 cut(s) 2306
BstBI TTCGAA 2 cut(s) 222, 1087
BstC8I GCNNGC 8 cut(s) 87, 715, 1018, 1105, 1277, 1292, 1987, 2497
BstDEI CTNAG 6 cut(s) 291, 574, 696, 1493, 1715, 2019
BstENI CCTNNNNNAGG 1 cut(s) 630
BstF5I GGATG 2 cut(s) 814, 1353
BstH2I RGCGCY 1 cut(s) 2354
BstHHI GCGC 2 cut(s) 886, 2353
BstKTI GATC 7 cut(s) 625, 658, 816, 1369, 1864, 2062, 2227
BstMAI GTCTC 5 cut(s) 180, 287, 612, 1006, 2115
BstMBI GATC 7 cut(s) 622, 655, 813, 1366, 1861, 2059, 2224
BstMWI GCNNNNNNNGC 2 cut(s) 396, 1521
BstNI CCWGG 4 cut(s) 243, 633, 1410, 2451
BstNSI RCATGY 6 cut(s) 1020, 1899, 2110, 2252, 2395, 2499
BstSCI CCNGG 4 cut(s) 241, 631, 1408, 2449
BstSFI CTRYAG 1 cut(s) 429
BstSLI GKGCMC 2 cut(s) 906, 2318
BstV1I GCAGC 5 cut(s) 898, 1119, 1259, 1364, 2458
BstV2I GAAGAC 2 cut(s) 1551, 2333
BstX2I RGATCY 1 cut(s) 813
BstXI CCANNNNNNTGG 2 cut(s) 1053, 1910
BstYI RGATCY 1 cut(s) 813
BstZ17I GTATAC 1 cut(s) 78
Bsu15I ATCGAT 1 cut(s) 175
BsuI GTATCC 1 cut(s) 2250
BsuRI GGCC 3 cut(s) 258, 729, 2037
BsuTUI ATCGAT 1 cut(s) 175
BtgZI GCGATG 1 cut(s) 1745
BtsCI GGATG 2 cut(s) 814, 1353
BtsI GCAGTG 2 cut(s) 1686, 2425
BtsIMutI CAGTG 5 cut(s) 279, 357, 1134, 1686, 2425
Cac8I GCNNGC 8 cut(s) 87, 715, 1018, 1105, 1277, 1292, 1987, 2497
CciI TCATGA 1 cut(s) 1827
CfoI GCGC 2 cut(s) 886, 2353
Cfr10I RCCGGY 1 cut(s) 2499
Cfr13I GGNCC 3 cut(s) 256, 843, 1637
ClaI ATCGAT 1 cut(s) 175
CspCI CAANNNNNGTGG 4 cut(s) 1153, 1188, 2067, 2102
DdeI CTNAG 6 cut(s) 291, 574, 696, 1493, 1715, 2019
DpnI GATC 7 cut(s) 624, 657, 815, 1368, 1863, 2061, 2226
DpnII GATC 7 cut(s) 622, 655, 813, 1366, 1861, 2059, 2224
Eco130I CCWWGG 1 cut(s) 1537
Eco32I GATATC 1 cut(s) 1252
Eco47I GGWCC 2 cut(s) 843, 1637
Eco57I CTGAAG 4 cut(s) 693, 1341, 2346, 2397
Eco88I CYCGRG 1 cut(s) 1111
EcoNI CCTNNNNNAGG 1 cut(s) 630
EcoRI GAATTC 4 cut(s) 1057, 1310, 1413, 1823
EcoRII CCWGG 4 cut(s) 241, 631, 1408, 2449
EcoRV GATATC 1 cut(s) 1252
EcoT14I CCWWGG 1 cut(s) 1537
EcoT22I ATGCAT 1 cut(s) 2254
ErhI CCWWGG 1 cut(s) 1537
Esp3I CGTCTC 2 cut(s) 180, 612
FalI AAGNNNNNCTT 2 cut(s) 1521, 1553
FaqI GGGAC 3 cut(s) 584, 1884, 2459
FauNDI CATATG 2 cut(s) 979, 1237
FbaI TGATCA 3 cut(s) 1366, 1861, 2059
FblI GTMKAC 1 cut(s) 77
Fnu4HI GCNGC 5 cut(s) 887, 1108, 1273, 1353, 2447
FokI GGATG 2 cut(s) 821, 1360
Fsp4HI GCNGC 5 cut(s) 887, 1108, 1273, 1353, 2447
FspBI CTAG 9 cut(s) 15, 155, 200, 374, 984, 1923, 1953, 1986, 2180
FspI TGCGCA 1 cut(s) 885
GlaI GCGC 2 cut(s) 885, 2352
GluI GCNGC 5 cut(s) 887, 1108, 1273, 1353, 2447
GsaI CCCAGC 1 cut(s) 1622
GsuI CTGGAG 3 cut(s) 61, 363, 615
HaeII RGCGCY 1 cut(s) 2354
HaeIII GGCC 3 cut(s) 258, 729, 2037
HapII CCGG 2 cut(s) 1320, 2500
HhaI GCGC 2 cut(s) 886, 2353
Hin6I GCGC 2 cut(s) 884, 2351
HinP1I GCGC 2 cut(s) 884, 2351
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HindIII AAGCTT 3 cut(s) 560, 1775, 1840
HpaI GTTAAC 1 cut(s) 505
HpaII CCGG 2 cut(s) 1320, 2500
HphI GGTGA 3 cut(s) 203, 1934, 2219
Hpy166II GTNNAC 8 cut(s) 78, 279, 505, 846, 904, 1134, 1645, 2316
Hpy188I TCNGA 8 cut(s) 687, 1075, 1360, 1496, 1511, 2029, 2152, 2326
Hpy8I GTNNAC 8 cut(s) 78, 279, 505, 846, 904, 1134, 1645, 2316
HpyAV CCTTC 7 cut(s) 136, 389, 740, 1018, 1619, 1658, 2249
HpyCH4III ACNGT 5 cut(s) 191, 283, 298, 355, 1579
HpyCH4IV ACGT 4 cut(s) 848, 1655, 2096, 2305
HpyF10VI GCNNNNNNNGC 2 cut(s) 396, 1521
HpyF3I CTNAG 6 cut(s) 291, 574, 696, 1493, 1715, 2019
HpySE526I ACGT 4 cut(s) 848, 1655, 2096, 2305
HspAI GCGC 2 cut(s) 884, 2351
Ksp22I TGATCA 3 cut(s) 1366, 1861, 2059
KspAI GTTAAC 1 cut(s) 505
Kzo9I GATC 7 cut(s) 622, 655, 813, 1366, 1861, 2059, 2224
LmnI GCTCC 3 cut(s) 612, 634, 692
Lsp1109I GCAGC 5 cut(s) 898, 1119, 1259, 1364, 2458
MaeI CTAG 9 cut(s) 15, 155, 200, 374, 984, 1923, 1953, 1986, 2180
MaeII ACGT 4 cut(s) 848, 1655, 2096, 2305
MaeIII GTNAC 6 cut(s) 355, 536, 577, 1610, 2102, 2425
MalI GATC 7 cut(s) 624, 657, 815, 1368, 1863, 2061, 2226
MbiI CCGCTC 1 cut(s) 949
MboI GATC 7 cut(s) 622, 655, 813, 1366, 1861, 2059, 2224
MflI RGATCY 1 cut(s) 813
MhlI GDGCHC 2 cut(s) 906, 2318
MlyI GAGTC 2 cut(s) 1515, 1943
MmeI TCCRAC 2 cut(s) 170, 2071
Mph1103I ATGCAT 1 cut(s) 2254
MseI TTAA 9 cut(s) 131, 504, 893, 1143, 1454, 1565, 2235, 2333, 2489
MslI CAYNNNNRTG 2 cut(s) 104, 2500
MspA1I CMGCKG 1 cut(s) 209
MspCI CTTAAG 2 cut(s) 130, 2488
MspI CCGG 2 cut(s) 1320, 2500
MspR9I CCNGG 4 cut(s) 243, 633, 1410, 2451
Mva1269I GAATGC 2 cut(s) 1202, 1791
MvaI CCWGG 4 cut(s) 243, 633, 1410, 2451
MwoI GCNNNNNNNGC 2 cut(s) 396, 1521
NdeI CATATG 2 cut(s) 979, 1237
NdeII GATC 7 cut(s) 622, 655, 813, 1366, 1861, 2059, 2224
NheI GCTAGC 1 cut(s) 1985
NmuCI GTSAC 2 cut(s) 355, 536
NsbI TGCGCA 1 cut(s) 885
NsiI ATGCAT 1 cut(s) 2254
NspI RCATGY 6 cut(s) 1020, 1899, 2110, 2252, 2395, 2499
NspV TTCGAA 2 cut(s) 222, 1087
PaeI GCATGC 2 cut(s) 1020, 2499
PagI TCATGA 1 cut(s) 1827
PciI ACATGT 1 cut(s) 2106
PctI GAATGC 2 cut(s) 1202, 1791
PfoI TCCNGGA 1 cut(s) 631
PkrI GCNGC 5 cut(s) 888, 1109, 1274, 1354, 2448
PleI GAGTC 2 cut(s) 1514, 1943
PpsI GAGTC 2 cut(s) 1514, 1943
Ppu21I YACGTR 1 cut(s) 2306
PscI ACATGT 1 cut(s) 2106
Psp6I CCWGG 4 cut(s) 241, 631, 1408, 2449
PspFI CCCAGC 1 cut(s) 1618
PspGI CCWGG 4 cut(s) 241, 631, 1408, 2449
PspPI GGNCC 3 cut(s) 256, 843, 1637
PstI CTGCAG 1 cut(s) 433
PsuI RGATCY 1 cut(s) 813
PvuII CAGCTG 1 cut(s) 209
RseI CAYNNNNRTG 2 cut(s) 104, 2500
SaqAI TTAA 9 cut(s) 131, 504, 893, 1143, 1454, 1565, 2235, 2333, 2489
SatI GCNGC 5 cut(s) 887, 1108, 1273, 1353, 2447
Sau3AI GATC 7 cut(s) 622, 655, 813, 1366, 1861, 2059, 2224
Sau96I GGNCC 3 cut(s) 256, 843, 1637
SchI GAGTC 2 cut(s) 1515, 1943
ScrFI CCNGG 4 cut(s) 243, 633, 1410, 2451
SduI GDGCHC 2 cut(s) 906, 2318
SfcI CTRYAG 1 cut(s) 429
SfuI TTCGAA 2 cut(s) 222, 1087
SinI GGWCC 2 cut(s) 843, 1637
SmiMI CAYNNNNRTG 2 cut(s) 104, 2500
SmlI CTYRAG 5 cut(s) 91, 130, 853, 1190, 2488
SmoI CTYRAG 5 cut(s) 91, 130, 853, 1190, 2488
SphI GCATGC 2 cut(s) 1020, 2499
SsiI CCGC 1 cut(s) 949
SspMI CTAG 9 cut(s) 15, 155, 200, 374, 984, 1923, 1953, 1986, 2180
StyD4I CCNGG 4 cut(s) 241, 631, 1408, 2449
StyI CCWWGG 1 cut(s) 1537
TaaI ACNGT 5 cut(s) 191, 283, 298, 355, 1579
TaiI ACGT 4 cut(s) 851, 1658, 2099, 2308
TaqI TCGA 6 cut(s) 67, 175, 222, 1087, 1884, 2141
TaqII GACCGA 1 cut(s) 378
TatI WGTACW 3 cut(s) 825, 1131, 1644
Tru1I TTAA 9 cut(s) 131, 504, 893, 1143, 1454, 1565, 2235, 2333, 2489
Tru9I TTAA 9 cut(s) 131, 504, 893, 1143, 1454, 1565, 2235, 2333, 2489
TscAI CASTG 5 cut(s) 286, 364, 1141, 1686, 2425
TseFI GTSAC 2 cut(s) 355, 536
TseI GCWGC 5 cut(s) 886, 1107, 1272, 1352, 2446
Tsp45I GTSAC 2 cut(s) 355, 536
TspGWI ACGGA 4 cut(s) 664, 1322, 1360, 1495
TspRI CASTG 5 cut(s) 286, 364, 1141, 1686, 2425
Vha464I CTTAAG 2 cut(s) 130, 2488
VneI GTGCAC 2 cut(s) 902, 2314
VpaK11BI GGWCC 2 cut(s) 843, 1637
XagI CCTNNNNNAGG 1 cut(s) 630
XapI RAATTY 9 cut(s) 415, 791, 1057, 1243, 1310, 1413, 1809, 1823, 2133
XbaI TCTAGA 1 cut(s) 1952
XceI RCATGY 6 cut(s) 1020, 1899, 2110, 2252, 2395, 2499
XcmI CCANNNNNNNNNTGG 2 cut(s) 1840, 1910
XmiI GTMKAC 1 cut(s) 77
XspI CTAG 9 cut(s) 15, 155, 200, 374, 984, 1923, 1953, 1986, 2180
Zsp2I ATGCAT 1 cut(s) 2254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.