Prupe.4G142300_v2.0.a1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000207
Physical Location & Seq
Reverse (-)
8163422 .. 8164717
1296 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G142300.1

Sequence Viewer

Length: 1296 bp
ATGCATTCTGATCAGAATATTGCCTTGAGAACCCTTTTTGTGTGCTCATTATTATTCTCCTTCGTGAGAACCTCCACTACAAATACACTAGACACTATCTCTCCAAATCAATATATTAGAGGTGATCAAACTCTAGTTTCAGCAGGTGGAACCTTTCAACTAGGATTCTTCAGCCCTGGTAAATTGACAAGCCGATACCTGGGGATATGGTACACTATTTCTAATGAGATAGTAGTATGGGTAGCCAACAGAGAAACACCACTTGATGATTCTTCAGGAGTTTTAAAGGTCACTGATCAAGGAGTTCTAGTCCTTCTCAATAGCTCAAACGGCGTTGTATGGTCATCCAACTCATCAAGAACTGTAGATAATCCAGTATCACAACTATTGGATTCGGGAAATCTTGTTGTGAAAAATGCAAACGAAACTAACCCTGATAACTTCCTGTGGCAGAGTTTTGATTATCCTTGTGACACATTCCTACCAGAAATGAAGCTTGGATGGGACTTCGTTACTGGTTTAGAAAGGTATGTTTCGTCTTGGAAGAGCACAGAAGATCCTGCTCGAGGAGAGTTTTCACTACGGATGACTCCTTATGGTTTACCACAACTTGTTGTTATGAAGGGAGCTAAGATAAAAACTCGATCAGGTTCATCGAACGGCCTTCGATTAACAGGATCTATCAGTCGACCAAACCCACTATCTGAGTTTGAATTTTTCTTGAACAAGGATGAGGTTTATTATGAGTACAGACTCCTAAACAAGTCTATGCTCTCGCGATATGCATTGAACCCATTTGGCATTGCACAGTGGTTCACATGGATTGAAAACACACATAGTTGGGAACCATTCTTTTCAACCCAACAAGACCAGTGTGAAATTTATGCCTTCTGTGGTTCATATTCTAGCTGCAACATCAGTAATGCTCCTGTATGTACATGCTTGAAGGGATTTATACCAAAATCGCCAGAACAATGGAATTCACAGAATTGGTCTGATGGGTGTGTTCGAAATACTCCATTATCTTGCAGCTATAATGATGGCTTCTTTAAATACACTAGCTTTAAATTGCCGGACACATCTTCCTCATGGTTTGATAAGAGCAAGAGCCTCAAGGAATGCAAGGGATTATGTTTGGAAAACTGCTCATGTACGTCATATGCAAATTTAGATATCAGGGATGGAGGAAGTGGCTGCTTGCTTTGGTTTGGAGACCTCACTGACATAAGAACCTTCCAATCTGATTCTCAAGACCTCTATATACGGCTGGCTTCTTCAGAACTAGGTGGCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

432

Amino Acids

48.46

Weight (kDa)

5.11

Isoelectric Point (pI)

41.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27300
fragaria_vesca FvH4_3g15930 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g21350 FvH4_3g21400 FvH4_3g21400 FvH4_6g12332
malus_domestica MD02G1167100.v1.1 MD02G1167200.v1.1 MD03G1185600.v1.1 MD05G1213900.v1.1 MD05G1214100.v1.1 MD05G1214200.v1.1 MD05G1214700.v1.1 MD05G1216300.v1.1 MD05G1216800.v1.1 MD05G1217300.v1.1 MD05G1218000.v1.1 MD11G1231100.v1.1 MD11G1231200.v1.1 MD11G1231400.v1.1 MD11G1231500.v1.1 MD11G1232400.v1.1 MD11G1232500.v1.1
prunus_persica Prupe.4G142300_v2.0.a1 Prupe.4G142400_v2.0.a1 Prupe.4G142500_v2.0.a1 Prupe.4G142600_v2.0.a1 Prupe.4G142800_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195100_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195500_v2.0.a1 Prupe.4G195700_v2.0.a1 Prupe.4G195900_v2.0.a1
pyrus_communis pycom03g16540 pycom05g19920 pycom11g20460 pycom11g20480 pycom11g20490 pycom11g20500 pycom11g20530
rosa_chinensis RchiOBHm_Chr2g0139311 RchiOBHm_Chr2g0139351 RchiOBHm_Chr5g0026801 RchiOBHm_Chr5g0026851 RchiOBHm_Chr5g0026891 RchiOBHm_Chr5g0026911 RchiOBHm_Chr5g0036571 RchiOBHm_Chr5g0036591 RchiOBHm_Chr5g0036721 RchiOBHm_Chr5g0036741 RchiOBHm_Chr7g0222981
rosa_laevigata RLG00000002006 RLG00000019807 RLG00000032964 RLG00000032968 RLG00000032969 RLG00000033706 RLG00000033708 RLG00000033712 RLG00000033716
rosa_multiflora Rmu_co8256651.1_g000001 Rmu_co8279377.1_g000001 Rmu_co8429919.1_g000001 Rmu_co8490103.1_g000001 Rmu_sc0000774.1_g000002 Rmu_sc0002525.1_g000012 Rmu_sc0004223.1_g000008 Rmu_sc0004390.1_g000002 Rmu_sc0006059.1_g000014 Rmu_sc0006059.1_g000024 Rmu_sc0006514.1_g000020 Rmu_sc0014333.1_g000002 Rmu_ssc0000172.1_g000018 Rmu_ssc0000172.1_g000029
rosa_roxburghii Rroxscaffold_1G00044230 Rroxscaffold_1G00044260 Rroxscaffold_1G00044320 Rroxscaffold_1G00044340 Rroxscaffold_1G00052560 Rroxscaffold_1G00052570 Rroxscaffold_1G00052660 Rroxscaffold_1G00052720 Rroxscaffold_2G00105450 Rroxscaffold_2G00105460
rosa_rugosa Rorug02G0356900 Rorug02G0357000 Rorug02G0357100 Rorug02G0357200 Rorug02G0357300 Rorug02G0357400 Rorug02G0357500 Rorug02G0357600 Rorug05G0095400 Rorug05G0095500 Rorug05G0095500 Rorug05G0095600 Rorug05G0095700 Rorug05G0095800 Rorug05G0157700 Rorug05G0157800
rosa_samantha Rh2AG406800 Rh2AG406900 Rh2BG417300 Rh2CG392900 Rh2DG426200 Rh5AG189300 Rh5AG249400 Rh5AG250100 Rh5BG186300 Rh5BG186900 Rh5BG187000 Rh5BG249500 Rh5BG250600 Rh5BG250900 Rh5BG251500 Rh5BG251800 Rh5CG205500 Rh5CG206300 Rh5CG206700 Rh5CG283000 Rh5CG283300 Rh5CG283500 Rh5DG188500 Rh5DG188700 Rh5DG258700 Rh5DG258900 Rh5DG259600 Rh5DG259900 Rh7CG375800 Rh7DG352500
rosa_wichuraiana Rw2G032960 Rw2G032970 Rw2G033220 Rw2G033230 Rw5G017130 Rw5G017230 Rw5G022940 Rw5G022960 Rw5G022980 Rw7G030420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 134
Acc36I ACCTGC 1 cut(s) 134
AccI GTMKAC 1 cut(s) 688
AccII CGCG 1 cut(s) 778
AclWI GGATC 2 cut(s) 551, 685
AcsI RAATTY 4 cut(s) 713, 879, 979, 1165
AcuI CTGAAG 3 cut(s) 154, 258, 1260
AfaI GTAC 4 cut(s) 212, 749, 937, 1153
AfiI CCNNNNNNNGG 2 cut(s) 199, 566
AgsI TTSAA 7 cut(s) 158, 713, 724, 790, 827, 858, 946
AjnI CCWGG 2 cut(s) 175, 198
AjuI GAANNNNNNNTTGG 2 cut(s) 480, 512
AluBI AGCT 6 cut(s) 324, 496, 629, 909, 1032, 1062
AluI AGCT 6 cut(s) 324, 496, 629, 909, 1032, 1062
Alw21I GWGCWC 2 cut(s) 47, 551
Alw26I GTCTC 1 cut(s) 1206
AlwI GGATC 2 cut(s) 551, 685
Ama87I CYCGRG 1 cut(s) 564
AoxI GGCC 1 cut(s) 661
ApeKI GCWGC 3 cut(s) 909, 1029, 1194
ApoI RAATTY 4 cut(s) 713, 879, 979, 1165
AsuHPI GGTGA 1 cut(s) 134
AsuII TTCGAA 1 cut(s) 1009
AvaI CYCGRG 1 cut(s) 564
Bbv12I GWGCWC 2 cut(s) 47, 551
BbvI GCAGC 3 cut(s) 896, 1041, 1181
BccI CCATC 4 cut(s) 495, 992, 1034, 1175
BceAI ACGGC 3 cut(s) 346, 676, 1280
BciT130I CCWGG 2 cut(s) 177, 200
BclI TGATCA 3 cut(s) 10, 124, 295
BcoDI GTCTC 1 cut(s) 1206
BfaI CTAG 7 cut(s) 89, 134, 161, 308, 906, 1059, 1283
BfmI CTRYAG 1 cut(s) 363
BfuAI ACCTGC 1 cut(s) 134
BisI GCNGC 3 cut(s) 910, 1030, 1195
BlsI GCNGC 3 cut(s) 911, 1031, 1196
Bme1390I CCNGG 2 cut(s) 177, 200
BmeT110I CYCGRG 1 cut(s) 564
BmiI GGNNCC 2 cut(s) 151, 846
BmrFI CCNGG 2 cut(s) 177, 200
Bpu14I TTCGAA 1 cut(s) 1009
BpuEI CTTGAG 3 cut(s) 46, 1097, 1233
BsaI GGTCTC 1 cut(s) 1206
BsaJI CCNNGG 2 cut(s) 175, 199
BsaXI ACNNNNNCTCC 4 cut(s) 85, 115, 294, 324
Bsc4I CCNNNNNNNGG 2 cut(s) 199, 566
Bse1I ACTGG 3 cut(s) 374, 520, 871
Bse3DI GCAATG 1 cut(s) 801
BseBI CCWGG 2 cut(s) 177, 200
BseDI CCNNGG 2 cut(s) 175, 199
BseGI GGATG 5 cut(s) 344, 506, 591, 736, 1186
BseLI CCNNNNNNNGG 2 cut(s) 199, 566
BseMI GCAATG 1 cut(s) 801
BseMII CTCAG 1 cut(s) 696
BseNI ACTGG 3 cut(s) 374, 520, 871
BseRI GAGGAG 1 cut(s) 582
BseXI GCAGC 3 cut(s) 896, 1041, 1181
Bsh1236I CGCG 1 cut(s) 778
BshFI GGCC 1 cut(s) 663
BsiHKAI GWGCWC 2 cut(s) 47, 551
BsiHKCI CYCGRG 1 cut(s) 564
BsiSI CCGG 1 cut(s) 1073
BslFI GGGAC 1 cut(s) 518
BslI CCNNNNNNNGG 2 cut(s) 199, 566
BsmAI GTCTC 1 cut(s) 1206
BsmFI GGGAC 1 cut(s) 518
BsmI GAATGC 2 cut(s) 4, 1124
BsnI GGCC 1 cut(s) 663
Bso31I GGTCTC 1 cut(s) 1206
BsoBI CYCGRG 1 cut(s) 564
Bsp119I TTCGAA 1 cut(s) 1009
Bsp1286I GDGCHC 2 cut(s) 47, 551
Bsp1407I TGTACA 1 cut(s) 935
Bsp143I GATC 6 cut(s) 10, 124, 295, 556, 644, 677
Bsp68I TCGCGA 1 cut(s) 778
BspANI GGCC 1 cut(s) 663
BspCNI CTCAG 1 cut(s) 697
BspFNI CGCG 1 cut(s) 778
BspLI GGNNCC 2 cut(s) 151, 846
BspMI ACCTGC 1 cut(s) 134
BspPI GGATC 2 cut(s) 551, 685
BspQI GCTCTTC 1 cut(s) 539
BspT104I TTCGAA 1 cut(s) 1009
BspTNI GGTCTC 1 cut(s) 1206
BsrDI GCAATG 1 cut(s) 801
BsrGI TGTACA 1 cut(s) 935
BsrI ACTGG 3 cut(s) 374, 520, 871
BssECI CCNNGG 2 cut(s) 175, 199
BssMI GATC 6 cut(s) 10, 124, 295, 556, 644, 677
Bst2UI CCWGG 2 cut(s) 177, 200
Bst4CI ACNGT 2 cut(s) 364, 810
Bst6I CTCTTC 1 cut(s) 539
BstAUI TGTACA 1 cut(s) 935
BstBI TTCGAA 1 cut(s) 1009
BstC8I GCNNGC 2 cut(s) 1199, 1269
BstDEI CTNAG 2 cut(s) 630, 705
BstENI CCTNNNNNAGG 1 cut(s) 564
BstF5I GGATG 5 cut(s) 344, 506, 591, 736, 1186
BstFNI CGCG 1 cut(s) 778
BstKTI GATC 6 cut(s) 13, 127, 298, 559, 647, 680
BstMAI GTCTC 1 cut(s) 1206
BstMBI GATC 6 cut(s) 10, 124, 295, 556, 644, 677
BstMWI GCNNNNNNNGC 1 cut(s) 330
BstNI CCWGG 2 cut(s) 177, 200
BstNSI RCATGY 1 cut(s) 942
BstSCI CCNGG 2 cut(s) 175, 198
BstSFI CTRYAG 1 cut(s) 363
BstUI CGCG 1 cut(s) 778
BstV1I GCAGC 3 cut(s) 896, 1041, 1181
BstX2I RGATCY 2 cut(s) 556, 677
BstXI CCANNNNNNTGG 1 cut(s) 975
BstYI RGATCY 2 cut(s) 556, 677
BsuRI GGCC 1 cut(s) 663
BtsCI GGATG 5 cut(s) 344, 506, 591, 736, 1186
BtsIMutI CAGTG 4 cut(s) 291, 815, 878, 1218
BtuMI TCGCGA 1 cut(s) 778
BveI ACCTGC 1 cut(s) 134
Cac8I GCNNGC 2 cut(s) 1199, 1269
Csp6I GTAC 4 cut(s) 211, 748, 936, 1152
CviAII CATG 4 cut(s) 819, 939, 1089, 1149
CviQI GTAC 4 cut(s) 211, 748, 936, 1152
DdeI CTNAG 2 cut(s) 630, 705
DpnI GATC 6 cut(s) 12, 126, 297, 558, 646, 679
DpnII GATC 6 cut(s) 10, 124, 295, 556, 644, 677
DraI TTTAAA 3 cut(s) 285, 1051, 1066
Eam1104I CTCTTC 1 cut(s) 539
EarI CTCTTC 1 cut(s) 539
Eco31I GGTCTC 1 cut(s) 1206
Eco32I GATATC 1 cut(s) 1174
Eco57I CTGAAG 3 cut(s) 154, 258, 1260
Eco88I CYCGRG 1 cut(s) 564
EcoNI CCTNNNNNAGG 1 cut(s) 564
EcoRI GAATTC 1 cut(s) 979
EcoRII CCWGG 2 cut(s) 175, 198
EcoRV GATATC 1 cut(s) 1174
EcoT22I ATGCAT 2 cut(s) 6, 787
FaeI CATG 4 cut(s) 822, 942, 1092, 1152
FaqI GGGAC 1 cut(s) 518
FatI CATG 4 cut(s) 818, 938, 1088, 1148
FauNDI CATATG 1 cut(s) 1159
FbaI TGATCA 3 cut(s) 10, 124, 295
FblI GTMKAC 1 cut(s) 688
Fnu4HI GCNGC 3 cut(s) 910, 1030, 1195
FokI GGATG 5 cut(s) 331, 513, 598, 743, 1193
Fsp4HI GCNGC 3 cut(s) 910, 1030, 1195
FspBI CTAG 7 cut(s) 89, 134, 161, 308, 906, 1059, 1283
GluI GCNGC 3 cut(s) 910, 1030, 1195
HaeIII GGCC 1 cut(s) 663
HapII CCGG 1 cut(s) 1073
Hin1II CATG 4 cut(s) 822, 942, 1092, 1152
HincII GTYRAC 1 cut(s) 689
HindII GTYRAC 1 cut(s) 689
HindIII AAGCTT 1 cut(s) 494
HinfI GANTC 6 cut(s) 165, 269, 392, 589, 753, 1244
HpaII CCGG 1 cut(s) 1073
HphI GGTGA 1 cut(s) 134
Hpy166II GTNNAC 4 cut(s) 213, 602, 689, 816
Hpy188I TCNGA 6 cut(s) 10, 15, 706, 997, 1243, 1279
Hpy188III TCNNGA 7 cut(s) 64, 276, 357, 396, 721, 777, 1250
Hpy8I GTNNAC 4 cut(s) 213, 602, 689, 816
HpyAV CCTTC 7 cut(s) 70, 323, 616, 674, 898, 940, 1243
HpyCH4III ACNGT 2 cut(s) 364, 810
HpyCH4IV ACGT 1 cut(s) 1154
HpyCH4V TGCA 8 cut(s) 4, 419, 785, 806, 912, 1029, 1122, 1163
HpyF10VI GCNNNNNNNGC 1 cut(s) 330
HpyF3I CTNAG 2 cut(s) 630, 705
HpySE526I ACGT 1 cut(s) 1154
Hsp92II CATG 4 cut(s) 822, 942, 1092, 1152
Ksp22I TGATCA 3 cut(s) 10, 124, 295
Kzo9I GATC 6 cut(s) 10, 124, 295, 556, 644, 677
LguI GCTCTTC 1 cut(s) 539
LmnI GCTCC 2 cut(s) 626, 931
Lsp1109I GCAGC 3 cut(s) 896, 1041, 1181
MaeI CTAG 7 cut(s) 89, 134, 161, 308, 906, 1059, 1283
MaeII ACGT 1 cut(s) 1154
MaeIII GTNAC 3 cut(s) 289, 470, 511
MalI GATC 6 cut(s) 12, 126, 297, 558, 646, 679
MboI GATC 6 cut(s) 10, 124, 295, 556, 644, 677
MboII GAAGA 6 cut(s) 160, 264, 556, 566, 1074, 1266
MflI RGATCY 2 cut(s) 556, 677
MhlI GDGCHC 2 cut(s) 47, 551
MluCI AATT 7 cut(s) 182, 713, 879, 979, 988, 1067, 1165
MlyI GAGTC 2 cut(s) 583, 747
MmeI TCCRAC 1 cut(s) 372
MnlI CCTC 9 cut(s) 82, 113, 560, 727, 1096, 1121, 1178, 1226, 1265
Mph1103I ATGCAT 2 cut(s) 6, 787
MseI TTAA 4 cut(s) 284, 671, 1050, 1065
MspI CCGG 1 cut(s) 1073
MspR9I CCNGG 2 cut(s) 177, 200
Mva1269I GAATGC 2 cut(s) 4, 1124
MvaI CCWGG 2 cut(s) 177, 200
MvnI CGCG 1 cut(s) 778
MwoI GCNNNNNNNGC 1 cut(s) 330
NdeI CATATG 1 cut(s) 1159
NdeII GATC 6 cut(s) 10, 124, 295, 556, 644, 677
NlaIII CATG 4 cut(s) 822, 942, 1092, 1152
NlaIV GGNNCC 2 cut(s) 151, 846
NmuCI GTSAC 2 cut(s) 289, 470
NruI TCGCGA 1 cut(s) 778
NsiI ATGCAT 2 cut(s) 6, 787
NspI RCATGY 1 cut(s) 942
NspV TTCGAA 1 cut(s) 1009
PaeR7I CTCGAG 1 cut(s) 564
PaqCI CACCTGC 1 cut(s) 134
PciSI GCTCTTC 1 cut(s) 539
PctI GAATGC 2 cut(s) 4, 1124
PfeI GAWTC 4 cut(s) 165, 269, 392, 1244
PkrI GCNGC 3 cut(s) 911, 1031, 1196
PleI GAGTC 2 cut(s) 583, 747
PpsI GAGTC 2 cut(s) 583, 747
Psp6I CCWGG 2 cut(s) 175, 198
PspGI CCWGG 2 cut(s) 175, 198
PspN4I GGNNCC 2 cut(s) 151, 846
PspXI VCTCGAGB 1 cut(s) 564
PsrI GAACNNNNNNTAC 2 cut(s) 61, 93
PsuI RGATCY 2 cut(s) 556, 677
RruI TCGCGA 1 cut(s) 778
RsaI GTAC 4 cut(s) 212, 749, 937, 1153
RsaNI GTAC 4 cut(s) 211, 748, 936, 1152
SalI GTCGAC 1 cut(s) 687
SapI GCTCTTC 1 cut(s) 539
SaqAI TTAA 4 cut(s) 284, 671, 1050, 1065
SatI GCNGC 3 cut(s) 910, 1030, 1195
Sau3AI GATC 6 cut(s) 10, 124, 295, 556, 644, 677
SchI GAGTC 2 cut(s) 583, 747
ScrFI CCNGG 2 cut(s) 177, 200
SduI GDGCHC 2 cut(s) 47, 551
SfcI CTRYAG 1 cut(s) 363
Sfr274I CTCGAG 1 cut(s) 564
SfuI TTCGAA 1 cut(s) 1009
SlaI CTCGAG 1 cut(s) 564
SmlI CTYRAG 4 cut(s) 25, 564, 1112, 1248
SmoI CTYRAG 4 cut(s) 25, 564, 1112, 1248
Sse9I AATT 7 cut(s) 182, 713, 879, 979, 988, 1067, 1165
SspI AATATT 1 cut(s) 19
SspMI CTAG 7 cut(s) 89, 134, 161, 308, 906, 1059, 1283
StyD4I CCNGG 2 cut(s) 175, 198
TaaI ACNGT 2 cut(s) 364, 810
TaiI ACGT 1 cut(s) 1157
TaqI TCGA 6 cut(s) 565, 643, 656, 667, 688, 1009
TasI AATT 7 cut(s) 182, 713, 879, 979, 988, 1067, 1165
TatI WGTACW 2 cut(s) 747, 935
TfiI GAWTC 4 cut(s) 165, 269, 392, 1244
Tru1I TTAA 4 cut(s) 284, 671, 1050, 1065
Tru9I TTAA 4 cut(s) 284, 671, 1050, 1065
TscAI CASTG 4 cut(s) 298, 815, 878, 1225
TseFI GTSAC 2 cut(s) 289, 470
TseI GCWGC 3 cut(s) 909, 1029, 1194
Tsp45I GTSAC 2 cut(s) 289, 470
TspDTI ATGAA 4 cut(s) 506, 635, 642, 888
TspGWI ACGGA 1 cut(s) 598
TspRI CASTG 4 cut(s) 298, 815, 878, 1225
XagI CCTNNNNNAGG 1 cut(s) 564
XapI RAATTY 4 cut(s) 713, 879, 979, 1165
XceI RCATGY 1 cut(s) 942
XhoI CTCGAG 1 cut(s) 564
XmiI GTMKAC 1 cut(s) 688
XspI CTAG 7 cut(s) 89, 134, 161, 308, 906, 1059, 1283
Zsp2I ATGCAT 2 cut(s) 6, 787
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.