Serine threonine-protein kinase
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| B_lectin | PF01453 | 2 - 84 | 1e-23 | D-mannose binding lectin |
| S_locus_glycop | PF00954 | 118 - 228 | 3.7e-27 | S-locus glycoprotein domain |
| PAN_2 | PF08276 | 249 - 317 | 3.8e-20 | PAN-like domain |
| Pkinase | PF00069 | 412 - 612 | 7.2e-43 | Protein kinase domain |
| PK_Tyr_Ser-Thr | PF07714 | 415 - 681 | 2e-45 | Protein tyrosine and serine/threonine kinase |
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 1820, 2045 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 709, 770 |
| Acc16I | TGCGCA | 1 cut(s) | 728 |
| AccB1I | GGYRCC | 1 cut(s) | 1741 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 371 |
| AccI | GTMKAC | 2 cut(s) | 661, 1098 |
| AccII | CGCG | 1 cut(s) | 525 |
| AccIII | TCCGGA | 2 cut(s) | 330, 961 |
| AciI | CCGC | 1 cut(s) | 1390 |
| AclI | AACGTT | 1 cut(s) | 1525 |
| AclWI | GGATC | 3 cut(s) | 984, 1844, 2025 |
| AcoI | YGGCCR | 3 cut(s) | 970, 1333, 2021 |
| AcsI | RAATTY | 5 cut(s) | 115, 253, 278, 697, 1650 |
| AcuI | CTGAAG | 3 cut(s) | 984, 1169, 1367 |
| AfaI | GTAC | 5 cut(s) | 88, 455, 464, 579, 1164 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 271, 272, 371, 968, 1587, 2129 |
| AflIII | ACRYGT | 1 cut(s) | 459 |
| AgsI | TTSAA | 9 cut(s) | 296, 492, 545, 790, 862, 1120, 1195, 1323, 1982 |
| AjnI | CCWGG | 6 cut(s) | 747, 972, 1176, 1467, 1580, 2086 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1245, 1277 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 854, 886 |
| Alw21I | GWGCWC | 4 cut(s) | 521, 1185, 1205, 1943 |
| Alw26I | GTCTC | 2 cut(s) | 1118, 1562 |
| AlwI | GGATC | 3 cut(s) | 984, 1844, 2025 |
| Ama87I | CYCGRG | 1 cut(s) | 1551 |
| Aor13HI | TCCGGA | 2 cut(s) | 330, 961 |
| AoxI | GGCC | 8 cut(s) | 361, 373, 404, 970, 1333, 1517, 1907, 2021 |
| ApeKI | GCWGC | 3 cut(s) | 744, 918, 1754 |
| ApoI | RAATTY | 5 cut(s) | 115, 253, 278, 697, 1650 |
| AsiSI | GCGATCGC | 1 cut(s) | 529 |
| AspLEI | GCGC | 3 cut(s) | 729, 923, 1061 |
| AspS9I | GGNCC | 4 cut(s) | 362, 1110, 1174, 1265 |
| AsuC2I | CCSGG | 1 cut(s) | 273 |
| AsuHPI | GGTGA | 2 cut(s) | 675, 947 |
| AsuII | TTCGAA | 2 cut(s) | 472, 988 |
| AvaI | CYCGRG | 1 cut(s) | 1551 |
| AvaII | GGWCC | 3 cut(s) | 1110, 1174, 1265 |
| AxyI | CCTNAGG | 2 cut(s) | 318, 2013 |
| BalI | TGGCCA | 2 cut(s) | 972, 1335 |
| BanI | GGYRCC | 1 cut(s) | 1741 |
| BanII | GRGCYC | 2 cut(s) | 1185, 1943 |
| BauI | CACGAG | 1 cut(s) | 1212 |
| BbsI | GAAGAC | 2 cut(s) | 267, 1034 |
| Bbv12I | GWGCWC | 4 cut(s) | 521, 1185, 1205, 1943 |
| BbvI | GCAGC | 3 cut(s) | 731, 905, 1741 |
| BccI | CCATC | 6 cut(s) | 134, 196, 201, 1010, 1286, 1754 |
| BciT130I | CCWGG | 6 cut(s) | 749, 974, 1178, 1469, 1582, 2088 |
| BciVI | GTATCC | 1 cut(s) | 2167 |
| BclI | TGATCA | 2 cut(s) | 595, 1486 |
| BcnI | CCSGG | 1 cut(s) | 273 |
| BcoDI | GTCTC | 2 cut(s) | 1118, 1562 |
| BfaI | CTAG | 5 cut(s) | 377, 582, 944, 1286, 1671 |
| BfuI | GTATCC | 1 cut(s) | 2167 |
| BglII | AGATCT | 1 cut(s) | 1603 |
| BisI | GCNGC | 3 cut(s) | 745, 919, 1755 |
| BlsI | GCNGC | 3 cut(s) | 746, 920, 1756 |
| Bme1390I | CCNGG | 7 cut(s) | 273, 749, 974, 1178, 1469, 1582, 2088 |
| Bme18I | GGWCC | 3 cut(s) | 1110, 1174, 1265 |
| BmeT110I | CYCGRG | 1 cut(s) | 1551 |
| BmgT120I | GGNCC | 4 cut(s) | 362, 1110, 1174, 1265 |
| BmiI | GGNNCC | 1 cut(s) | 1743 |
| BmrFI | CCNGG | 7 cut(s) | 273, 749, 974, 1178, 1469, 1582, 2088 |
| BmsI | GCATC | 5 cut(s) | 401, 581, 790, 955, 2042 |
| BoxI | GACNNNNGTC | 1 cut(s) | 267 |
| BpiI | GAAGAC | 2 cut(s) | 267, 1034 |
| BpmI | CTGGAG | 3 cut(s) | 1199, 1704, 1832 |
| Bpu10I | CCTNAGC | 2 cut(s) | 740, 1586 |
| Bpu14I | TTCGAA | 2 cut(s) | 472, 988 |
| BpuMI | CCSGG | 1 cut(s) | 273 |
| BsaI | GGTCTC | 1 cut(s) | 1562 |
| BsaJI | CCNNGG | 3 cut(s) | 272, 365, 1336 |
| BsaWI | WCCGGW | 3 cut(s) | 190, 330, 961 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 271, 272, 371, 968, 1587, 2129 |
| Bse1I | ACTGG | 4 cut(s) | 647, 713, 1687, 1702 |
| Bse21I | CCTNAGG | 2 cut(s) | 318, 2013 |
| BseAI | TCCGGA | 2 cut(s) | 330, 961 |
| BseBI | CCWGG | 6 cut(s) | 749, 974, 1178, 1469, 1582, 2088 |
| BseDI | CCNNGG | 3 cut(s) | 272, 365, 1336 |
| BseGI | GGATG | 5 cut(s) | 212, 725, 805, 970, 2008 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 271, 272, 371, 968, 1587, 2129 |
| BseMII | CTCAG | 2 cut(s) | 309, 2004 |
| BseNI | ACTGG | 4 cut(s) | 647, 713, 1687, 1702 |
| BseRI | GAGGAG | 2 cut(s) | 1550, 1701 |
| BseXI | GCAGC | 3 cut(s) | 731, 905, 1741 |
| Bsh1236I | CGCG | 1 cut(s) | 525 |
| Bsh1285I | CGRYCG | 1 cut(s) | 529 |
| BshFI | GGCC | 8 cut(s) | 363, 375, 406, 972, 1335, 1519, 1909, 2023 |
| BshNI | GGYRCC | 1 cut(s) | 1741 |
| BsiEI | CGRYCG | 1 cut(s) | 529 |
| BsiHKAI | GWGCWC | 4 cut(s) | 521, 1185, 1205, 1943 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1551 |
| BsiSI | CCGG | 5 cut(s) | 191, 272, 331, 797, 962 |
| BslFI | GGGAC | 3 cut(s) | 224, 1294, 1738 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 271, 272, 371, 968, 1587, 2129 |
| BsmAI | GTCTC | 2 cut(s) | 1118, 1562 |
| BsmFI | GGGAC | 3 cut(s) | 224, 1294, 1738 |
| BsmI | GAATGC | 4 cut(s) | 380, 848, 1080, 1977 |
| BsnI | GGCC | 8 cut(s) | 363, 375, 406, 972, 1335, 1519, 1909, 2023 |
| Bso31I | GGTCTC | 1 cut(s) | 1562 |
| BsoBI | CYCGRG | 1 cut(s) | 1551 |
| Bsp119I | TTCGAA | 2 cut(s) | 472, 988 |
| Bsp1286I | GDGCHC | 4 cut(s) | 521, 1185, 1205, 1943 |
| Bsp13I | TCCGGA | 2 cut(s) | 330, 961 |
| Bsp1407I | TGTACA | 1 cut(s) | 462 |
| Bsp19I | CCATGG | 1 cut(s) | 365 |
| BspACI | CCGC | 1 cut(s) | 1390 |
| BspANI | GGCC | 8 cut(s) | 363, 375, 406, 972, 1335, 1519, 1909, 2023 |
| BspCNI | CTCAG | 2 cut(s) | 310, 2005 |
| BspEI | TCCGGA | 2 cut(s) | 330, 961 |
| BspFNI | CGCG | 1 cut(s) | 525 |
| BspHI | TCATGA | 1 cut(s) | 1353 |
| BspLI | GGNNCC | 1 cut(s) | 1743 |
| BspPI | GGATC | 3 cut(s) | 984, 1844, 2025 |
| BspT104I | TTCGAA | 2 cut(s) | 472, 988 |
| BspT107I | GGYRCC | 1 cut(s) | 1741 |
| BspTNI | GGTCTC | 1 cut(s) | 1562 |
| BsrGI | TGTACA | 1 cut(s) | 462 |
| BsrI | ACTGG | 4 cut(s) | 647, 713, 1687, 1702 |
| BssECI | CCNNGG | 3 cut(s) | 272, 365, 1336 |
| BssSI | CACGAG | 1 cut(s) | 1212 |
| BssT1I | CCWWGG | 2 cut(s) | 365, 1336 |
| Bst2BI | CACGAG | 1 cut(s) | 1212 |
| Bst2UI | CCWGG | 6 cut(s) | 749, 974, 1178, 1469, 1582, 2088 |
| Bst4CI | ACNGT | 2 cut(s) | 869, 1509 |
| Bst6I | CTCTTC | 2 cut(s) | 1307, 1422 |
| BstAUI | TGTACA | 1 cut(s) | 462 |
| BstBI | TTCGAA | 2 cut(s) | 472, 988 |
| BstC8I | GCNNGC | 4 cut(s) | 970, 1421, 2000, 2119 |
| BstDEI | CTNAG | 9 cut(s) | 72, 318, 401, 678, 740, 1081, 1586, 1710, 2013 |
| BstDSI | CCRYGG | 1 cut(s) | 365 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2127 |
| BstF5I | GGATG | 5 cut(s) | 212, 725, 805, 970, 2008 |
| BstFNI | CGCG | 1 cut(s) | 525 |
| BstHHI | GCGC | 3 cut(s) | 729, 923, 1061 |
| BstMAI | GTCTC | 2 cut(s) | 1118, 1562 |
| BstMCI | CGRYCG | 1 cut(s) | 529 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 1332 |
| BstNI | CCWGG | 6 cut(s) | 749, 974, 1178, 1469, 1582, 2088 |
| BstNSI | RCATGY | 2 cut(s) | 463, 1456 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 267 |
| BstSCI | CCNGG | 7 cut(s) | 271, 747, 972, 1176, 1467, 1580, 2086 |
| BstUI | CGCG | 1 cut(s) | 525 |
| BstV1I | GCAGC | 3 cut(s) | 731, 905, 1741 |
| BstV2I | GAAGAC | 2 cut(s) | 267, 1034 |
| BstX2I | RGATCY | 2 cut(s) | 976, 1603 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 693 |
| BstYI | RGATCY | 2 cut(s) | 976, 1603 |
| Bsu36I | CCTNAGG | 2 cut(s) | 318, 2013 |
| BsuI | GTATCC | 1 cut(s) | 2167 |
| BsuRI | GGCC | 8 cut(s) | 363, 375, 406, 972, 1335, 1519, 1909, 2023 |
| BtgI | CCRYGG | 1 cut(s) | 365 |
| BtsCI | GGATG | 5 cut(s) | 212, 725, 805, 970, 2008 |
| BtsI | GCAGTG | 1 cut(s) | 1068 |
| BtsIMutI | CAGTG | 2 cut(s) | 391, 1068 |
| Cac8I | GCNNGC | 4 cut(s) | 970, 1421, 2000, 2119 |
| CciI | TCATGA | 1 cut(s) | 1353 |
| CfoI | GCGC | 3 cut(s) | 729, 923, 1061 |
| Cfr13I | GGNCC | 4 cut(s) | 362, 1110, 1174, 1265 |
| CsiI | ACCWGGT | 1 cut(s) | 2086 |
| Csp6I | GTAC | 5 cut(s) | 87, 454, 463, 578, 1163 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1520, 1555 |
| CviQI | GTAC | 5 cut(s) | 87, 454, 463, 578, 1163 |
| DdeI | CTNAG | 9 cut(s) | 72, 318, 401, 678, 740, 1081, 1586, 1710, 2013 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 709, 770 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 709, 770 |
| EaeI | YGGCCR | 3 cut(s) | 970, 1333, 2021 |
| Eam1104I | CTCTTC | 2 cut(s) | 1307, 1422 |
| EarI | CTCTTC | 2 cut(s) | 1307, 1422 |
| Ecl136II | GAGCTC | 2 cut(s) | 1183, 1941 |
| Eco130I | CCWWGG | 2 cut(s) | 365, 1336 |
| Eco147I | AGGCCT | 2 cut(s) | 406, 1909 |
| Eco24I | GRGCYC | 2 cut(s) | 1185, 1943 |
| Eco31I | GGTCTC | 1 cut(s) | 1562 |
| Eco32I | GATATC | 3 cut(s) | 20, 898, 949 |
| Eco47I | GGWCC | 3 cut(s) | 1110, 1174, 1265 |
| Eco53kI | GAGCTC | 2 cut(s) | 1183, 1941 |
| Eco57I | CTGAAG | 3 cut(s) | 984, 1169, 1367 |
| Eco81I | CCTNAGG | 2 cut(s) | 318, 2013 |
| Eco88I | CYCGRG | 1 cut(s) | 1551 |
| EcoICRI | GAGCTC | 2 cut(s) | 1183, 1941 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2127 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1174 |
| EcoRI | GAATTC | 2 cut(s) | 278, 697 |
| EcoRII | CCWGG | 6 cut(s) | 747, 972, 1176, 1467, 1580, 2086 |
| EcoRV | GATATC | 3 cut(s) | 20, 898, 949 |
| EcoT14I | CCWWGG | 2 cut(s) | 365, 1336 |
| EcoT22I | ATGCAT | 2 cut(s) | 313, 574 |
| EcoT38I | GRGCYC | 2 cut(s) | 1185, 1943 |
| ErhI | CCWWGG | 2 cut(s) | 365, 1336 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1974, 2006 |
| FaqI | GGGAC | 3 cut(s) | 224, 1294, 1738 |
| FauNDI | CATATG | 1 cut(s) | 1729 |
| FbaI | TGATCA | 2 cut(s) | 595, 1486 |
| FblI | GTMKAC | 2 cut(s) | 661, 1098 |
| Fnu4HI | GCNGC | 3 cut(s) | 745, 919, 1755 |
| FokI | GGATG | 5 cut(s) | 219, 732, 812, 977, 1995 |
| FriOI | GRGCYC | 2 cut(s) | 1185, 1943 |
| Fsp4HI | GCNGC | 3 cut(s) | 745, 919, 1755 |
| FspBI | CTAG | 5 cut(s) | 377, 582, 944, 1286, 1671 |
| FspI | TGCGCA | 1 cut(s) | 728 |
| GlaI | GCGC | 3 cut(s) | 728, 922, 1060 |
| GluI | GCNGC | 3 cut(s) | 745, 919, 1755 |
| GsuI | CTGGAG | 3 cut(s) | 1199, 1704, 1832 |
| HaeIII | GGCC | 8 cut(s) | 363, 375, 406, 972, 1335, 1519, 1909, 2023 |
| HapII | CCGG | 5 cut(s) | 191, 272, 331, 797, 962 |
| HhaI | GCGC | 3 cut(s) | 729, 923, 1061 |
| Hin6I | GCGC | 3 cut(s) | 727, 921, 1059 |
| HinP1I | GCGC | 3 cut(s) | 727, 921, 1059 |
| HindIII | AAGCTT | 4 cut(s) | 160, 889, 956, 1244 |
| HinfI | GANTC | 7 cut(s) | 107, 669, 1405, 1577, 1642, 1925, 2138 |
| HpaII | CCGG | 5 cut(s) | 191, 272, 331, 797, 962 |
| HphI | GGTGA | 2 cut(s) | 675, 947 |
| Hpy166II | GTNNAC | 7 cut(s) | 60, 662, 780, 820, 832, 1099, 1273 |
| Hpy188III | TCNNGA | 9 cut(s) | 111, 331, 962, 1071, 1186, 1354, 1723, 1853, 2012 |
| Hpy8I | GTNNAC | 7 cut(s) | 60, 662, 780, 820, 832, 1099, 1273 |
| HpyAV | CCTTC | 5 cut(s) | 183, 1247, 1821, 1895, 1899 |
| HpyCH4III | ACNGT | 2 cut(s) | 869, 1509 |
| HpyCH4IV | ACGT | 3 cut(s) | 1095, 1525, 1972 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 1332 |
| HpyF3I | CTNAG | 9 cut(s) | 72, 318, 401, 678, 740, 1081, 1586, 1710, 2013 |
| HpySE526I | ACGT | 3 cut(s) | 1095, 1525, 1972 |
| HspAI | GCGC | 3 cut(s) | 727, 921, 1059 |
| Kpn2I | TCCGGA | 2 cut(s) | 330, 961 |
| Ksp22I | TGATCA | 2 cut(s) | 595, 1486 |
| LmnI | GCTCC | 3 cut(s) | 614, 1180, 1188 |
| Lsp1109I | GCAGC | 3 cut(s) | 731, 905, 1741 |
| LweI | GCATC | 5 cut(s) | 401, 581, 790, 955, 2042 |
| MabI | ACCWGGT | 1 cut(s) | 2086 |
| MaeI | CTAG | 5 cut(s) | 377, 582, 944, 1286, 1671 |
| MaeII | ACGT | 3 cut(s) | 1095, 1525, 1972 |
| MaeIII | GTNAC | 6 cut(s) | 133, 186, 638, 681, 711, 1968 |
| MflI | RGATCY | 2 cut(s) | 976, 1603 |
| MhlI | GDGCHC | 4 cut(s) | 521, 1185, 1205, 1943 |
| MlsI | TGGCCA | 2 cut(s) | 972, 1335 |
| MluNI | TGGCCA | 2 cut(s) | 972, 1335 |
| MnlI | CCTC | 8 cut(s) | 313, 555, 902, 1165, 1308, 1571, 1679, 2008 |
| Mox20I | TGGCCA | 2 cut(s) | 972, 1335 |
| Mph1103I | ATGCAT | 2 cut(s) | 313, 574 |
| MroI | TCCGGA | 2 cut(s) | 330, 961 |
| MscI | TGGCCA | 2 cut(s) | 972, 1335 |
| MseI | TTAA | 5 cut(s) | 228, 503, 1344, 1365, 1679 |
| MslI | CAYNNNNRTG | 2 cut(s) | 718, 989 |
| Msp20I | TGGCCA | 2 cut(s) | 972, 1335 |
| MspI | CCGG | 5 cut(s) | 191, 272, 331, 797, 962 |
| MspR9I | CCNGG | 7 cut(s) | 273, 749, 974, 1178, 1469, 1582, 2088 |
| Mva1269I | GAATGC | 4 cut(s) | 380, 848, 1080, 1977 |
| MvaI | CCWGG | 6 cut(s) | 749, 974, 1178, 1469, 1582, 2088 |
| MvnI | CGCG | 1 cut(s) | 525 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 1332 |
| NciI | CCSGG | 1 cut(s) | 273 |
| NcoI | CCATGG | 1 cut(s) | 365 |
| NdeI | CATATG | 1 cut(s) | 1729 |
| NlaIV | GGNNCC | 1 cut(s) | 1743 |
| NmuCI | GTSAC | 4 cut(s) | 133, 638, 681, 711 |
| NsbI | TGCGCA | 1 cut(s) | 728 |
| NsiI | ATGCAT | 2 cut(s) | 313, 574 |
| NspI | RCATGY | 2 cut(s) | 463, 1456 |
| NspV | TTCGAA | 2 cut(s) | 472, 988 |
| PagI | TCATGA | 1 cut(s) | 1353 |
| PceI | AGGCCT | 2 cut(s) | 406, 1909 |
| PciI | ACATGT | 1 cut(s) | 459 |
| PctI | GAATGC | 4 cut(s) | 380, 848, 1080, 1977 |
| PfeI | GAWTC | 7 cut(s) | 107, 669, 1405, 1577, 1642, 1925, 2138 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 371 |
| PkrI | GCNGC | 3 cut(s) | 746, 920, 1756 |
| Ple19I | CGATCG | 1 cut(s) | 529 |
| PpuMI | RGGWCCY | 1 cut(s) | 1174 |
| PscI | ACATGT | 1 cut(s) | 459 |
| PshAI | GACNNNNGTC | 1 cut(s) | 267 |
| PsiI | TTATAA | 2 cut(s) | 1820, 2045 |
| Psp124BI | GAGCTC | 2 cut(s) | 1185, 1943 |
| Psp1406I | AACGTT | 1 cut(s) | 1525 |
| Psp5II | RGGWCCY | 1 cut(s) | 1174 |
| Psp6I | CCWGG | 6 cut(s) | 747, 972, 1176, 1467, 1580, 2086 |
| PspGI | CCWGG | 6 cut(s) | 747, 972, 1176, 1467, 1580, 2086 |
| PspN4I | GGNNCC | 1 cut(s) | 1743 |
| PspPI | GGNCC | 4 cut(s) | 362, 1110, 1174, 1265 |
| PspPPI | RGGWCCY | 1 cut(s) | 1174 |
| PsuI | RGATCY | 2 cut(s) | 976, 1603 |
| PvuI | CGATCG | 1 cut(s) | 529 |
| RgaI | GCGATCGC | 1 cut(s) | 529 |
| RsaI | GTAC | 5 cut(s) | 88, 455, 464, 579, 1164 |
| RsaNI | GTAC | 5 cut(s) | 87, 454, 463, 578, 1163 |
| RseI | CAYNNNNRTG | 2 cut(s) | 718, 989 |
| SacI | GAGCTC | 2 cut(s) | 1185, 1943 |
| SaqAI | TTAA | 5 cut(s) | 228, 503, 1344, 1365, 1679 |
| SatI | GCNGC | 3 cut(s) | 745, 919, 1755 |
| Sau96I | GGNCC | 4 cut(s) | 362, 1110, 1174, 1265 |
| ScrFI | CCNGG | 7 cut(s) | 273, 749, 974, 1178, 1469, 1582, 2088 |
| SduI | GDGCHC | 4 cut(s) | 521, 1185, 1205, 1943 |
| SexAI | ACCWGGT | 1 cut(s) | 2086 |
| SfaAI | GCGATCGC | 1 cut(s) | 529 |
| SfaNI | GCATC | 5 cut(s) | 401, 581, 790, 955, 2042 |
| SfuI | TTCGAA | 2 cut(s) | 472, 988 |
| SgfI | GCGATCGC | 1 cut(s) | 529 |
| SinI | GGWCC | 3 cut(s) | 1110, 1174, 1265 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 718, 989 |
| SseBI | AGGCCT | 2 cut(s) | 406, 1909 |
| SsiI | CCGC | 1 cut(s) | 1390 |
| SspI | AATATT | 2 cut(s) | 350, 1677 |
| SspMI | CTAG | 5 cut(s) | 377, 582, 944, 1286, 1671 |
| SstI | GAGCTC | 2 cut(s) | 1185, 1943 |
| StuI | AGGCCT | 2 cut(s) | 406, 1909 |
| StyD4I | CCNGG | 7 cut(s) | 271, 747, 972, 1176, 1467, 1580, 2086 |
| StyI | CCWWGG | 2 cut(s) | 365, 1336 |
| TaaI | ACNGT | 2 cut(s) | 869, 1509 |
| TaiI | ACGT | 3 cut(s) | 1098, 1528, 1975 |
| TaqI | TCGA | 5 cut(s) | 165, 426, 472, 701, 988 |
| TatI | WGTACW | 3 cut(s) | 86, 453, 462 |
| TfiI | GAWTC | 7 cut(s) | 107, 669, 1405, 1577, 1642, 1925, 2138 |
| Tru1I | TTAA | 5 cut(s) | 228, 503, 1344, 1365, 1679 |
| Tru9I | TTAA | 5 cut(s) | 228, 503, 1344, 1365, 1679 |
| TscAI | CASTG | 2 cut(s) | 391, 1068 |
| TseFI | GTSAC | 4 cut(s) | 133, 638, 681, 711 |
| TseI | GCWGC | 3 cut(s) | 744, 918, 1754 |
| Tsp45I | GTSAC | 4 cut(s) | 133, 638, 681, 711 |
| TspRI | CASTG | 2 cut(s) | 391, 1068 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 371 |
| VpaK11BI | GGWCC | 3 cut(s) | 1110, 1174, 1265 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2127 |
| XapI | RAATTY | 5 cut(s) | 115, 253, 278, 697, 1650 |
| XceI | RCATGY | 2 cut(s) | 463, 1456 |
| XcmI | CCANNNNNNNNNTGG | 4 cut(s) | 1465, 1538, 1871, 2167 |
| XmiI | GTMKAC | 2 cut(s) | 661, 1098 |
| XspI | CTAG | 5 cut(s) | 377, 582, 944, 1286, 1671 |
| Zsp2I | ATGCAT | 2 cut(s) | 313, 574 |