RLG00000032969

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000207
Physical Location & Seq
Reverse (-)
21538062 .. 21541810
3749 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032969

Sequence Viewer

Length: 2319 bp
ATGAAAACCATTTTTCTGTGCTTTTTCTTATTTTCCTTCCTAAGAACCTCAAATACACTAGACTCTATCACTCCAACTCAATTTATTAAAGATGGTCAAACTCTGGTTTCAGCAGGTGGAAGCTATGAACTGGGATTCTTTAGTCCCGGTGAAGTGAAGGGCAGATACTTGGGAATATGGTACACCTTTTCTACTGAGACAGTGGTATGGGTAGCCAACAGAGAAGTACCACTTGATGATTCTTCAGGAGTTTTAAAGGTCACTGATCAGGGAGTTTTAGTCCTTCTCAATAGCTCAAATGGCACTGCATGGTCCTCCAACTTATTGAGAACTGCAGGGAATCCAGTCTCGCAACTCTTGGATTCGGGAAATCTTGTTGTGAAAGATGGAAAAGAAACTAATCCTGATAACTTCTTGTGGCAGAGTTTTGATTATCCTTTTGACACACACCTGCCAGAAATGAAGCTTGGTTGGAATTTAGTCACTGGTTTAGATAGGTATGTCTCGTCTTGGAGGAGCACAGAAGATCCTGCTCAAGGAGACTTTTCACTACGGATGGATACTCATGGTTTCCCACAGATTTTTATTATGAAGGGAGCTAAGATACTGACTAGAGCAGGGCCATGGAACGGCGTTGGATTAACTGGATATCAAGGAAGGCCAAATCCAGTAGCAAATTTTGAATTTGTGTTGACTAAGAATGAAGTCTATTTTGAGTACACACTCATCAACAGGTCAATGTTTGCAAGATTTGTGTTGAACCAGTATGGCCTTGCACAGTGGTTTACCTGGACAGATTACTCACACAGTTGGGAACCTTTCGTGTCATCCCAAGCAGATCAGTGTGATAATTATGCCTTTTGTGGTGCTAATGCTAGATGTAATGTCAGTAATACTCCTGTATGTGCATGCTTGAAAGGGTTTGTACCAAAATCTCCGAAAGATTGGAACTCAACAAATTGGTCTGAGGGATGTGTTCGGAAGACTCCATTAGCTTGCAGCTCTGCAGATGGCTTCTCGAAGTACAGTAACTTTAAACTGCCAGACACATCTTCTTCCTGGTATGACAAGAGCATGAGCCTCAAGGAATGCCAGGGATTATGTTTGCGAAACTGTTCATGTACTGCATGTGCAAATTTAGATGTCAGGGAAGGTGGAAGTGGCTGCTTGCTTTGGTTTGGGAACCTCAATGACATTAGAGAATTCACCTCTGATTATCAAGACCTCTATATACGGCTGGCTGCTTCAGACCTAGATCCTATTGTGAAAAAGAGCAAGTCCAGCAAGAAAAAGCTAGCAGGAATTCCAATCGGCTCATCTGTACTTCTTGTGGGAATGCTAATAGTTGGGTTTGTTTTGTATAAACGGAAGAGGAAACTCAGAAAGCAAGAAGCCAGAAGAAAGCTGGATTCCAGAAAGGGGGATTACCGAGAGGAAATTGCAGTAAAGAGGCTCTCAAAGAACTCTGGACAGGGATTGAGAGAGTTTAAAAACGAAGTTCTACTGATAGCCAAACTTCAGCATAGGAATCTTGTAAAGCTTCTTGGTTGCTGCATTCAAGAAGATGAAGAAATTTTAGTATATGAATTCATGCCTAACAGAAACTTGGACTTCTTTATCTTTGATCAAGAAAGAGAGAAGTTTCTCGACTGGCCCACGTGCTTCCACATTATTGATGGAATTGCTCGAGGGCTTCTTTATCTCCATCAAGACTCTAGATTAAGAATTATCCATAGGGACATGAAAGCTAGCAATATCTTGCTAGACAATGATATGAACCCAAAGATTTCGGACTTCGGCCTGGCTAAAACATTTGGGGGTGATCAAAGTGAAGACAGTACAAATAGAGTAGTTGGAACACATGGTTATATGGCTCCTGAATATGCAGTAGATGGAACTTTCTCAATGAAATCTGATGTATTTAGCTTTGGAGTTATTATGCTAGAGATATTGAGCAGGAAGCAGAACAGGGGATTTTGTCATCCAGATCACCATCTTAACCTTCTTGGACATGCATGGACACTATGGAATCAAAATAATCCTCTGGAAATGGTTGATGTGACGTTATGTGATTCTTGCAACATAACCGAAGTGTTAAGGTGTCTTCATGTGGGCCTGTTATGTGTGCAACAAGTACCTGAAGATAGACCAAGCATGTCATCTGTGGTTCTAATGTTGGGCAGTGAAGTTAAGTTGCCTTCACCAAAGCAGCCTGGTTTTTACACTGGACGGACTGTGACAGTCCCCGAATCAACATCAACAACGACCGACTTGTTTTCAACAAATAATTGCAGCACAATGTTTCTAGAGGCTCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

773

Amino Acids

87.01

Weight (kDa)

7.13

Isoelectric Point (pI)

39.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 67 - 172 4.7e-36 D-mannose binding lectin
S_locus_glycop PF00954 205 - 312 4.8e-25 S-locus glycoprotein domain
PAN_2 PF08276 333 - 399 8.1e-19 PAN-like domain
PK_Tyr_Ser-Thr PF07714 472 - 723 4.8e-41 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 475 - 655 4.1e-38 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27300
fragaria_vesca FvH4_3g15930 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g21350 FvH4_3g21400 FvH4_3g21400 FvH4_6g12332
malus_domestica MD02G1167100.v1.1 MD02G1167200.v1.1 MD03G1185600.v1.1 MD05G1213900.v1.1 MD05G1214100.v1.1 MD05G1214200.v1.1 MD05G1214700.v1.1 MD05G1216300.v1.1 MD05G1216800.v1.1 MD05G1217300.v1.1 MD05G1218000.v1.1 MD11G1231100.v1.1 MD11G1231200.v1.1 MD11G1231400.v1.1 MD11G1231500.v1.1 MD11G1232400.v1.1 MD11G1232500.v1.1
prunus_persica Prupe.4G142300_v2.0.a1 Prupe.4G142400_v2.0.a1 Prupe.4G142500_v2.0.a1 Prupe.4G142600_v2.0.a1 Prupe.4G142800_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195100_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195500_v2.0.a1 Prupe.4G195700_v2.0.a1 Prupe.4G195900_v2.0.a1
pyrus_communis pycom03g16540 pycom05g19920 pycom11g20460 pycom11g20480 pycom11g20490 pycom11g20500 pycom11g20530
rosa_chinensis RchiOBHm_Chr2g0139311 RchiOBHm_Chr2g0139351 RchiOBHm_Chr5g0026801 RchiOBHm_Chr5g0026851 RchiOBHm_Chr5g0026891 RchiOBHm_Chr5g0026911 RchiOBHm_Chr5g0036571 RchiOBHm_Chr5g0036591 RchiOBHm_Chr5g0036721 RchiOBHm_Chr5g0036741 RchiOBHm_Chr7g0222981
rosa_laevigata RLG00000002006 RLG00000019807 RLG00000032964 RLG00000032968 RLG00000032969 RLG00000033706 RLG00000033708 RLG00000033712 RLG00000033716
rosa_multiflora Rmu_co8256651.1_g000001 Rmu_co8279377.1_g000001 Rmu_co8429919.1_g000001 Rmu_co8490103.1_g000001 Rmu_sc0000774.1_g000002 Rmu_sc0002525.1_g000012 Rmu_sc0004223.1_g000008 Rmu_sc0004390.1_g000002 Rmu_sc0006059.1_g000014 Rmu_sc0006059.1_g000024 Rmu_sc0006514.1_g000020 Rmu_sc0014333.1_g000002 Rmu_ssc0000172.1_g000018 Rmu_ssc0000172.1_g000029
rosa_roxburghii Rroxscaffold_1G00044230 Rroxscaffold_1G00044260 Rroxscaffold_1G00044320 Rroxscaffold_1G00044340 Rroxscaffold_1G00052560 Rroxscaffold_1G00052570 Rroxscaffold_1G00052660 Rroxscaffold_1G00052720 Rroxscaffold_2G00105450 Rroxscaffold_2G00105460
rosa_rugosa Rorug02G0356900 Rorug02G0357000 Rorug02G0357100 Rorug02G0357200 Rorug02G0357300 Rorug02G0357400 Rorug02G0357500 Rorug02G0357600 Rorug05G0095400 Rorug05G0095500 Rorug05G0095500 Rorug05G0095600 Rorug05G0095700 Rorug05G0095800 Rorug05G0157700 Rorug05G0157800
rosa_samantha Rh2AG406800 Rh2AG406900 Rh2BG417300 Rh2CG392900 Rh2DG426200 Rh5AG189300 Rh5AG249400 Rh5AG250100 Rh5BG186300 Rh5BG186900 Rh5BG187000 Rh5BG249500 Rh5BG250600 Rh5BG250900 Rh5BG251500 Rh5BG251800 Rh5CG205500 Rh5CG206300 Rh5CG206700 Rh5CG283000 Rh5CG283300 Rh5CG283500 Rh5DG188500 Rh5DG188700 Rh5DG258700 Rh5DG258900 Rh5DG259600 Rh5DG259900 Rh7CG375800 Rh7DG352500
rosa_wichuraiana Rw2G032960 Rw2G032970 Rw2G033220 Rw2G033230 Rw5G017130 Rw5G017230 Rw5G022940 Rw5G022960 Rw5G022980 Rw7G030420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 104, 459
Acc36I ACCTGC 2 cut(s) 104, 459
AclWI GGATC 2 cut(s) 521, 1250
AcsI RAATTY 8 cut(s) 475, 676, 683, 1135, 1202, 1302, 1572, 1586
AcuI CTGAAG 4 cut(s) 228, 1230, 1502, 2160
AcvI CACGTG 1 cut(s) 1659
AfaI GTAC 9 cut(s) 182, 228, 719, 927, 1025, 1123, 1323, 1840, 2136
AfiI CCNNNNNNNGG 3 cut(s) 536, 629, 1419
AgsI TTSAA 5 cut(s) 683, 760, 916, 1559, 2280
AjnI CCWGG 5 cut(s) 788, 1058, 1092, 1800, 2212
AjuI GAANNNNNNNTTGG 2 cut(s) 450, 482
Alw21I GWGCWC 1 cut(s) 521
Alw26I GTCTC 4 cut(s) 191, 352, 508, 534
AlwI GGATC 2 cut(s) 521, 1250
Ama87I CYCGRG 1 cut(s) 1686
AoxI GGCC 6 cut(s) 620, 659, 769, 1652, 1798, 2113
ApeKI GCWGC 6 cut(s) 999, 1164, 1241, 1551, 2209, 2292
ApoI RAATTY 8 cut(s) 475, 676, 683, 1135, 1202, 1302, 1572, 1586
Asp700I GAANNNNTTC 1 cut(s) 1114
AspS9I GGNCC 4 cut(s) 312, 620, 1653, 2113
AsuC2I CCSGG 1 cut(s) 147
AsuHPI GGTGA 5 cut(s) 161, 1198, 1832, 1982, 2193
AsuNHI GCTAGC 2 cut(s) 1294, 1748
AvaI CYCGRG 1 cut(s) 1686
AvaII GGWCC 1 cut(s) 312
BarI GAAGNNNNNNTAC 2 cut(s) 149, 181
BbrPI CACGTG 1 cut(s) 1659
BbsI GAAGAC 3 cut(s) 989, 1839, 2096
Bbv12I GWGCWC 1 cut(s) 521
BbvI GCAGC 6 cut(s) 1011, 1151, 1228, 1538, 2221, 2304
BccI CCATC 8 cut(s) 86, 380, 550, 1004, 1670, 1713, 1886, 2001
BceAI ACGGC 2 cut(s) 646, 1250
BciT130I CCWGG 5 cut(s) 790, 1060, 1094, 1802, 2214
BciVI GTATCC 1 cut(s) 553
BclI TGATCA 3 cut(s) 265, 1624, 1822
BcnI CCSGG 1 cut(s) 147
BcoDI GTCTC 4 cut(s) 191, 352, 508, 534
BfmI CTRYAG 2 cut(s) 333, 1005
BfuAI ACCTGC 2 cut(s) 104, 459
BfuI GTATCC 1 cut(s) 553
BisI GCNGC 6 cut(s) 1000, 1165, 1242, 1552, 2210, 2293
BlsI GCNGC 6 cut(s) 1001, 1166, 1243, 1553, 2211, 2294
Bme1390I CCNGG 6 cut(s) 147, 790, 1060, 1094, 1802, 2214
Bme18I GGWCC 1 cut(s) 312
BmeT110I CYCGRG 1 cut(s) 1686
BmgT120I GGNCC 4 cut(s) 312, 620, 1653, 2113
BmiI GGNNCC 3 cut(s) 816, 1184, 1875
BmrFI CCNGG 6 cut(s) 147, 790, 1060, 1094, 1802, 2214
BmrI ACTGGG 1 cut(s) 140
BmtI GCTAGC 2 cut(s) 1298, 1752
BmuI ACTGGG 1 cut(s) 140
BpiI GAAGAC 3 cut(s) 989, 1839, 2096
BpuEI CTTGAG 2 cut(s) 519, 1067
BpuMI CCSGG 1 cut(s) 147
BsaAI YACGTR 1 cut(s) 1659
BsaBI GATNNNNATC 1 cut(s) 1992
BsaJI CCNNGG 2 cut(s) 623, 1093
BsaXI ACNNNNNCTCC 2 cut(s) 264, 294
Bsc4I CCNNNNNNNGG 3 cut(s) 536, 629, 1419
Bse1I ACTGG 8 cut(s) 135, 344, 490, 649, 668, 763, 1655, 2230
Bse8I GATNNNNATC 1 cut(s) 1992
BseBI CCWGG 5 cut(s) 790, 1060, 1094, 1802, 2214
BseDI CCNNGG 2 cut(s) 623, 1093
BseGI GGATG 4 cut(s) 561, 827, 977, 1981
BseJI GATNNNNATC 1 cut(s) 1992
BseLI CCNNNNNNNGG 3 cut(s) 536, 629, 1419
BseMII CTCAG 3 cut(s) 186, 957, 1393
BseNI ACTGG 8 cut(s) 135, 344, 490, 649, 668, 763, 1655, 2230
BseRI GAGGAG 1 cut(s) 529
BseXI GCAGC 6 cut(s) 1011, 1151, 1228, 1538, 2221, 2304
Bsh1285I CGRYCG 1 cut(s) 2268
BshFI GGCC 6 cut(s) 622, 661, 771, 1654, 1800, 2115
BsiEI CGRYCG 1 cut(s) 2268
BsiHKAI GWGCWC 1 cut(s) 521
BsiHKCI CYCGRG 1 cut(s) 1686
BsiSI CCGG 1 cut(s) 147
BslFI GGGAC 3 cut(s) 129, 1751, 2228
BslI CCNNNNNNNGG 3 cut(s) 536, 629, 1419
BsmAI GTCTC 4 cut(s) 191, 352, 508, 534
BsmFI GGGAC 3 cut(s) 129, 1751, 2228
BsmI GAATGC 3 cut(s) 1094, 1341, 1554
BsnI GGCC 6 cut(s) 622, 661, 771, 1654, 1800, 2115
BsoBI CYCGRG 1 cut(s) 1686
Bsp1286I GDGCHC 1 cut(s) 521
Bsp143I GATC 7 cut(s) 265, 526, 838, 1255, 1624, 1822, 1987
Bsp19I CCATGG 1 cut(s) 623
BspANI GGCC 6 cut(s) 622, 661, 771, 1654, 1800, 2115
BspCNI CTCAG 3 cut(s) 187, 958, 1392
BspLI GGNNCC 3 cut(s) 816, 1184, 1875
BspMAI CTGCAG 2 cut(s) 337, 1009
BspMI ACCTGC 2 cut(s) 104, 459
BspOI GCTAGC 2 cut(s) 1298, 1752
BspPI GGATC 2 cut(s) 521, 1250
BsrI ACTGG 8 cut(s) 135, 344, 490, 649, 668, 763, 1655, 2230
BssECI CCNNGG 2 cut(s) 623, 1093
BssMI GATC 7 cut(s) 265, 526, 838, 1255, 1624, 1822, 1987
BssT1I CCWWGG 1 cut(s) 623
Bst2UI CCWGG 5 cut(s) 790, 1060, 1094, 1802, 2214
Bst4CI ACNGT 8 cut(s) 202, 780, 809, 1028, 1115, 1838, 2236, 2242
Bst6I CTCTTC 1 cut(s) 1364
BstBAI YACGTR 1 cut(s) 1659
BstC8I GCNNGC 7 cut(s) 910, 997, 1169, 1239, 1296, 1750, 2314
BstDEI CTNAG 6 cut(s) 41, 195, 600, 696, 966, 1379
BstDSI CCRYGG 1 cut(s) 623
BstENI CCTNNNNNAGG 1 cut(s) 534
BstF5I GGATG 4 cut(s) 561, 827, 977, 1981
BstKTI GATC 7 cut(s) 268, 529, 841, 1258, 1627, 1825, 1990
BstMAI GTCTC 4 cut(s) 191, 352, 508, 534
BstMBI GATC 7 cut(s) 265, 526, 838, 1255, 1624, 1822, 1987
BstMCI CGRYCG 1 cut(s) 2268
BstMWI GCNNNNNNNGC 2 cut(s) 300, 1281
BstNI CCWGG 5 cut(s) 790, 1060, 1094, 1802, 2214
BstNSI RCATGY 4 cut(s) 912, 1131, 2015, 2158
BstSCI CCNGG 6 cut(s) 145, 788, 1058, 1092, 1800, 2212
BstSFI CTRYAG 2 cut(s) 333, 1005
BstV1I GCAGC 6 cut(s) 1011, 1151, 1228, 1538, 2221, 2304
BstV2I GAAGAC 3 cut(s) 989, 1839, 2096
BstX2I RGATCY 2 cut(s) 526, 1255
BstYI RGATCY 2 cut(s) 526, 1255
BsuI GTATCC 1 cut(s) 553
BsuRI GGCC 6 cut(s) 622, 661, 771, 1654, 1800, 2115
BtgI CCRYGG 1 cut(s) 623
BtsCI GGATG 4 cut(s) 561, 827, 977, 1981
BtsI GCAGTG 2 cut(s) 303, 2188
BtsIMutI CAGTG 8 cut(s) 207, 261, 303, 483, 785, 848, 2188, 2223
BveI ACCTGC 2 cut(s) 104, 459
Cac8I GCNNGC 7 cut(s) 910, 997, 1169, 1239, 1296, 1750, 2314
Cfr13I GGNCC 4 cut(s) 312, 620, 1653, 2113
Csp6I GTAC 9 cut(s) 181, 227, 718, 926, 1024, 1122, 1322, 1839, 2135
CviQI GTAC 9 cut(s) 181, 227, 718, 926, 1024, 1122, 1322, 1839, 2135
DdeI CTNAG 6 cut(s) 41, 195, 600, 696, 966, 1379
DpnI GATC 7 cut(s) 267, 528, 840, 1257, 1626, 1824, 1989
DpnII GATC 7 cut(s) 265, 526, 838, 1255, 1624, 1822, 1987
DraI TTTAAA 3 cut(s) 255, 1036, 1489
Eam1104I CTCTTC 1 cut(s) 1364
EarI CTCTTC 1 cut(s) 1364
Eco130I CCWWGG 1 cut(s) 623
Eco32I GATATC 1 cut(s) 650
Eco47I GGWCC 1 cut(s) 312
Eco57I CTGAAG 4 cut(s) 228, 1230, 1502, 2160
Eco72I CACGTG 1 cut(s) 1659
Eco88I CYCGRG 1 cut(s) 1686
EcoNI CCTNNNNNAGG 1 cut(s) 534
EcoRI GAATTC 3 cut(s) 1202, 1302, 1586
EcoRII CCWGG 5 cut(s) 788, 1058, 1092, 1800, 2212
EcoRV GATATC 1 cut(s) 650
EcoT14I CCWWGG 1 cut(s) 623
EcoT22I ATGCAT 1 cut(s) 2017
ErhI CCWWGG 1 cut(s) 623
FalI AAGNNNNNCTT 2 cut(s) 216, 248
FaqI GGGAC 3 cut(s) 129, 1751, 2228
FbaI TGATCA 3 cut(s) 265, 1624, 1822
Fnu4HI GCNGC 6 cut(s) 1000, 1165, 1242, 1552, 2210, 2293
FokI GGATG 4 cut(s) 568, 814, 984, 1968
Fsp4HI GCNGC 6 cut(s) 1000, 1165, 1242, 1552, 2210, 2293
GluI GCNGC 6 cut(s) 1000, 1165, 1242, 1552, 2210, 2293
HaeIII GGCC 6 cut(s) 622, 661, 771, 1654, 1800, 2115
HapII CCGG 1 cut(s) 147
HincII GTYRAC 1 cut(s) 693
HindII GTYRAC 1 cut(s) 693
HindIII AAGCTT 2 cut(s) 464, 1538
HpaII CCGG 1 cut(s) 147
HphI GGTGA 5 cut(s) 161, 1198, 1832, 1982, 2193
Hpy166II GTNNAC 4 cut(s) 183, 693, 720, 786
Hpy188I TCNGA 8 cut(s) 939, 967, 981, 1213, 1249, 1382, 1792, 1915
Hpy8I GTNNAC 4 cut(s) 183, 693, 720, 786
HpyAV CCTTC 8 cut(s) 46, 151, 293, 586, 651, 1145, 2012, 2208
HpyCH4III ACNGT 8 cut(s) 202, 780, 809, 1028, 1115, 1838, 2236, 2242
HpyCH4IV ACGT 2 cut(s) 1658, 2063
HpyF10VI GCNNNNNNNGC 2 cut(s) 300, 1281
HpyF3I CTNAG 6 cut(s) 41, 195, 600, 696, 966, 1379
HpySE526I ACGT 2 cut(s) 1658, 2063
Ksp22I TGATCA 3 cut(s) 265, 1624, 1822
Kzo9I GATC 7 cut(s) 265, 526, 838, 1255, 1624, 1822, 1987
LmnI GCTCC 3 cut(s) 516, 596, 1879
Lsp1109I GCAGC 6 cut(s) 1011, 1151, 1228, 1538, 2221, 2304
MaeII ACGT 2 cut(s) 1658, 2063
MaeIII GTNAC 5 cut(s) 259, 481, 1028, 2059, 2236
MalI GATC 7 cut(s) 267, 528, 840, 1257, 1626, 1824, 1989
MboI GATC 7 cut(s) 265, 526, 838, 1255, 1624, 1822, 1987
MflI RGATCY 2 cut(s) 526, 1255
MhlI GDGCHC 1 cut(s) 521
MlyI GAGTC 3 cut(s) 56, 979, 1706
MmeI TCCRAC 5 cut(s) 98, 342, 452, 616, 1834
Mph1103I ATGCAT 1 cut(s) 2017
MroXI GAANNNNTTC 1 cut(s) 1114
MseI TTAA 9 cut(s) 87, 254, 641, 1035, 1488, 1721, 1998, 2096, 2190
MspI CCGG 1 cut(s) 147
MspR9I CCNGG 6 cut(s) 147, 790, 1060, 1094, 1802, 2214
Mva1269I GAATGC 3 cut(s) 1094, 1341, 1554
MvaI CCWGG 5 cut(s) 790, 1060, 1094, 1802, 2214
MwoI GCNNNNNNNGC 2 cut(s) 300, 1281
NciI CCSGG 1 cut(s) 147
NcoI CCATGG 1 cut(s) 623
NdeII GATC 7 cut(s) 265, 526, 838, 1255, 1624, 1822, 1987
NheI GCTAGC 2 cut(s) 1294, 1748
NlaIV GGNNCC 3 cut(s) 816, 1184, 1875
NmuCI GTSAC 4 cut(s) 259, 481, 2059, 2236
NsiI ATGCAT 1 cut(s) 2017
NspI RCATGY 4 cut(s) 912, 1131, 2015, 2158
PaeI GCATGC 1 cut(s) 912
PaeR7I CTCGAG 1 cut(s) 1686
PaqCI CACCTGC 2 cut(s) 104, 459
PctI GAATGC 3 cut(s) 1094, 1341, 1554
PdmI GAANNNNTTC 1 cut(s) 1114
PfeI GAWTC 9 cut(s) 135, 239, 340, 362, 1409, 1528, 2029, 2072, 2249
PkrI GCNGC 6 cut(s) 1001, 1166, 1243, 1553, 2211, 2294
PleI GAGTC 3 cut(s) 56, 979, 1706
PmaCI CACGTG 1 cut(s) 1659
PmlI CACGTG 1 cut(s) 1659
PpsI GAGTC 3 cut(s) 56, 979, 1706
Ppu21I YACGTR 1 cut(s) 1659
Psp6I CCWGG 5 cut(s) 788, 1058, 1092, 1800, 2212
PspCI CACGTG 1 cut(s) 1659
PspGI CCWGG 5 cut(s) 788, 1058, 1092, 1800, 2212
PspN4I GGNNCC 3 cut(s) 816, 1184, 1875
PspPI GGNCC 4 cut(s) 312, 620, 1653, 2113
PspXI VCTCGAGB 1 cut(s) 1686
PsrI GAACNNNNNNTAC 2 cut(s) 37, 69
PstI CTGCAG 2 cut(s) 337, 1009
PsuI RGATCY 2 cut(s) 526, 1255
RsaI GTAC 9 cut(s) 182, 228, 719, 927, 1025, 1123, 1323, 1840, 2136
RsaNI GTAC 9 cut(s) 181, 227, 718, 926, 1024, 1122, 1322, 1839, 2135
SaqAI TTAA 9 cut(s) 87, 254, 641, 1035, 1488, 1721, 1998, 2096, 2190
SatI GCNGC 6 cut(s) 1000, 1165, 1242, 1552, 2210, 2293
Sau3AI GATC 7 cut(s) 265, 526, 838, 1255, 1624, 1822, 1987
Sau96I GGNCC 4 cut(s) 312, 620, 1653, 2113
SchI GAGTC 3 cut(s) 56, 979, 1706
ScrFI CCNGG 6 cut(s) 147, 790, 1060, 1094, 1802, 2214
SduI GDGCHC 1 cut(s) 521
SfcI CTRYAG 2 cut(s) 333, 1005
Sfr274I CTCGAG 1 cut(s) 1686
SinI GGWCC 1 cut(s) 312
SlaI CTCGAG 1 cut(s) 1686
SmlI CTYRAG 3 cut(s) 534, 1082, 1686
SmoI CTYRAG 3 cut(s) 534, 1082, 1686
SphI GCATGC 1 cut(s) 912
StyD4I CCNGG 6 cut(s) 145, 788, 1058, 1092, 1800, 2212
StyI CCWWGG 1 cut(s) 623
TaaI ACNGT 8 cut(s) 202, 780, 809, 1028, 1115, 1838, 2236, 2242
TaiI ACGT 2 cut(s) 1661, 2066
TaqI TCGA 3 cut(s) 1019, 1647, 1687
TaqII GACCGA 1 cut(s) 2282
TatI WGTACW 5 cut(s) 717, 1023, 1121, 1321, 1838
TfiI GAWTC 9 cut(s) 135, 239, 340, 362, 1409, 1528, 2029, 2072, 2249
Tru1I TTAA 9 cut(s) 87, 254, 641, 1035, 1488, 1721, 1998, 2096, 2190
Tru9I TTAA 9 cut(s) 87, 254, 641, 1035, 1488, 1721, 1998, 2096, 2190
TscAI CASTG 8 cut(s) 207, 268, 310, 490, 785, 848, 2188, 2230
TseFI GTSAC 4 cut(s) 259, 481, 2059, 2236
TseI GCWGC 6 cut(s) 999, 1164, 1241, 1551, 2209, 2292
Tsp45I GTSAC 4 cut(s) 259, 481, 2059, 2236
TspGWI ACGGA 3 cut(s) 568, 1381, 2245
TspRI CASTG 8 cut(s) 207, 268, 310, 490, 785, 848, 2188, 2230
VpaK11BI GGWCC 1 cut(s) 312
XagI CCTNNNNNAGG 1 cut(s) 534
XapI RAATTY 8 cut(s) 475, 676, 683, 1135, 1202, 1302, 1572, 1586
XbaI TCTAGA 2 cut(s) 1715, 2305
XceI RCATGY 4 cut(s) 912, 1131, 2015, 2158
XcmI CCANNNNNNNNNTGG 2 cut(s) 1402, 1673
XhoI CTCGAG 1 cut(s) 1686
XmnI GAANNNNTTC 1 cut(s) 1114
Zsp2I ATGCAT 1 cut(s) 2017
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.