Serine threonine-protein kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 1004 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 698, 1244 |
| AccI | GTMKAC | 1 cut(s) | 780 |
| AciI | CCGC | 2 cut(s) | 25, 888 |
| AclWI | GGATC | 1 cut(s) | 1260 |
| AcsI | RAATTY | 5 cut(s) | 249, 448, 580, 973, 1052 |
| AcuI | CTGAAG | 2 cut(s) | 199, 1260 |
| AcyI | GRCGYC | 1 cut(s) | 882 |
| AdeI | CACNNNGTG | 1 cut(s) | 869 |
| AfaI | GTAC | 8 cut(s) | 63, 257, 346, 456, 685, 791, 847, 1189 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 480, 599, 698, 1244 |
| AflIII | ACRYGT | 1 cut(s) | 786 |
| AgsI | TTSAA | 9 cut(s) | 21, 124, 186, 623, 800, 832, 949, 1066, 1138 |
| AjnI | CCWGG | 3 cut(s) | 517, 598, 1023 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 227, 259 |
| AleI | CACNNNNGTG | 1 cut(s) | 95 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1130, 1162 |
| AluBI | AGCT | 8 cut(s) | 198, 205, 404, 433, 489, 1020, 1167, 1241 |
| AluI | AGCT | 8 cut(s) | 198, 205, 404, 433, 489, 1020, 1167, 1241 |
| Alw26I | GTCTC | 3 cut(s) | 55, 963, 993 |
| AlwI | GGATC | 1 cut(s) | 1260 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1244 |
| Ama87I | CYCGRG | 1 cut(s) | 230 |
| AoxI | GGCC | 3 cut(s) | 688, 700, 731 |
| ApeKI | GCWGC | 3 cut(s) | 1020, 1194, 1241 |
| ApoI | RAATTY | 5 cut(s) | 249, 448, 580, 973, 1052 |
| AseI | ATTAAT | 1 cut(s) | 312 |
| AspLEI | GCGC | 2 cut(s) | 1005, 1199 |
| AspS9I | GGNCC | 1 cut(s) | 689 |
| AsuHPI | GGTGA | 4 cut(s) | 711, 926, 1105, 1223 |
| AsuII | TTCGAA | 1 cut(s) | 1264 |
| AvaI | CYCGRG | 1 cut(s) | 230 |
| AxyI | CCTNAGG | 1 cut(s) | 645 |
| BauI | CACGAG | 1 cut(s) | 849 |
| BbsI | GAAGAC | 2 cut(s) | 66, 1310 |
| BbvI | GCAGC | 3 cut(s) | 1007, 1181, 1228 |
| BccI | CCATC | 6 cut(s) | 161, 523, 528, 973, 1034, 1208 |
| BciT130I | CCWGG | 3 cut(s) | 519, 600, 1025 |
| BcoDI | GTCTC | 3 cut(s) | 55, 963, 993 |
| BfaI | CTAG | 1 cut(s) | 992 |
| BfmI | CTRYAG | 1 cut(s) | 1347 |
| BisI | GCNGC | 3 cut(s) | 1021, 1195, 1242 |
| BlsI | GCNGC | 3 cut(s) | 1022, 1196, 1243 |
| BmcAI | AGTACT | 2 cut(s) | 456, 847 |
| Bme1390I | CCNGG | 3 cut(s) | 519, 600, 1025 |
| BmeT110I | CYCGRG | 1 cut(s) | 230 |
| BmgT120I | GGNCC | 1 cut(s) | 689 |
| BmiI | GGNNCC | 3 cut(s) | 130, 472, 892 |
| BmrFI | CCNGG | 3 cut(s) | 519, 600, 1025 |
| BmrI | ACTGGG | 2 cut(s) | 148, 263 |
| BmsI | GCATC | 3 cut(s) | 290, 336, 454 |
| BmuI | ACTGGG | 2 cut(s) | 148, 263 |
| BoxI | GACNNNNGTC | 1 cut(s) | 594 |
| BpiI | GAAGAC | 2 cut(s) | 66, 1310 |
| Bpu14I | TTCGAA | 1 cut(s) | 1264 |
| BpuEI | CTTGAG | 4 cut(s) | 562, 802, 945, 1097 |
| BsaBI | GATNNNNATC | 1 cut(s) | 639 |
| BsaHI | GRCGYC | 1 cut(s) | 882 |
| BsaI | GGTCTC | 1 cut(s) | 993 |
| BsaJI | CCNNGG | 2 cut(s) | 177, 692 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 480, 599, 698, 1244 |
| Bse1I | ACTGG | 8 cut(s) | 37, 154, 269, 691, 843, 923, 989, 1195 |
| Bse21I | CCTNAGG | 1 cut(s) | 645 |
| Bse3DI | GCAATG | 1 cut(s) | 1317 |
| Bse8I | GATNNNNATC | 1 cut(s) | 639 |
| BseBI | CCWGG | 3 cut(s) | 519, 600, 1025 |
| BseDI | CCNNGG | 2 cut(s) | 177, 692 |
| BseGI | GGATG | 3 cut(s) | 264, 539, 1001 |
| BseJI | GATNNNNATC | 1 cut(s) | 639 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 480, 599, 698, 1244 |
| BseMI | GCAATG | 1 cut(s) | 1317 |
| BseMII | CTCAG | 5 cut(s) | 92, 414, 582, 636, 695 |
| BseNI | ACTGG | 8 cut(s) | 37, 154, 269, 691, 843, 923, 989, 1195 |
| BseRI | GAGGAG | 2 cut(s) | 1194, 1197 |
| BseXI | GCAGC | 3 cut(s) | 1007, 1181, 1228 |
| BseYI | CCCAGC | 1 cut(s) | 1244 |
| BshFI | GGCC | 3 cut(s) | 690, 702, 733 |
| BsiHKCI | CYCGRG | 1 cut(s) | 230 |
| BsiSI | CCGG | 1 cut(s) | 324 |
| BslFI | GGGAC | 3 cut(s) | 132, 363, 551 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 480, 599, 698, 1244 |
| BsmAI | GTCTC | 3 cut(s) | 55, 963, 993 |
| BsmBI | CGTCTC | 1 cut(s) | 55 |
| BsmFI | GGGAC | 3 cut(s) | 132, 363, 551 |
| BsmI | GAATGC | 1 cut(s) | 1124 |
| BsnI | GGCC | 3 cut(s) | 690, 702, 733 |
| Bso31I | GGTCTC | 1 cut(s) | 993 |
| BsoBI | CYCGRG | 1 cut(s) | 230 |
| Bsp119I | TTCGAA | 1 cut(s) | 1264 |
| Bsp1407I | TGTACA | 1 cut(s) | 789 |
| Bsp143I | GATC | 3 cut(s) | 853, 1213, 1252 |
| Bsp19I | CCATGG | 1 cut(s) | 692 |
| BspACI | CCGC | 2 cut(s) | 25, 888 |
| BspANI | GGCC | 3 cut(s) | 690, 702, 733 |
| BspCNI | CTCAG | 5 cut(s) | 91, 413, 581, 637, 696 |
| BspLI | GGNNCC | 3 cut(s) | 130, 472, 892 |
| BspPI | GGATC | 1 cut(s) | 1260 |
| BspT104I | TTCGAA | 1 cut(s) | 1264 |
| BspTNI | GGTCTC | 1 cut(s) | 993 |
| BsrDI | GCAATG | 1 cut(s) | 1317 |
| BsrGI | TGTACA | 1 cut(s) | 789 |
| BsrI | ACTGG | 8 cut(s) | 37, 154, 269, 691, 843, 923, 989, 1195 |
| BssECI | CCNNGG | 2 cut(s) | 177, 692 |
| BssMI | GATC | 3 cut(s) | 853, 1213, 1252 |
| BssNAI | GTATAC | 1 cut(s) | 781 |
| BssNI | GRCGYC | 1 cut(s) | 882 |
| BssSI | CACGAG | 1 cut(s) | 849 |
| BssT1I | CCWWGG | 2 cut(s) | 177, 692 |
| Bst1107I | GTATAC | 1 cut(s) | 781 |
| Bst2BI | CACGAG | 1 cut(s) | 849 |
| Bst2UI | CCWGG | 3 cut(s) | 519, 600, 1025 |
| Bst4CI | ACNGT | 6 cut(s) | 61, 280, 344, 427, 867, 1145 |
| BstACI | GRCGYC | 1 cut(s) | 882 |
| BstAUI | TGTACA | 1 cut(s) | 789 |
| BstBI | TTCGAA | 1 cut(s) | 1264 |
| BstDEI | CTNAG | 7 cut(s) | 78, 400, 458, 568, 645, 704, 954 |
| BstDSI | CCRYGG | 1 cut(s) | 692 |
| BstF5I | GGATG | 3 cut(s) | 264, 539, 1001 |
| BstHHI | GCGC | 2 cut(s) | 1005, 1199 |
| BstKTI | GATC | 3 cut(s) | 856, 1216, 1255 |
| BstMAI | GTCTC | 3 cut(s) | 55, 963, 993 |
| BstMBI | GATC | 3 cut(s) | 853, 1213, 1252 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 410, 419 |
| BstNI | CCWGG | 3 cut(s) | 519, 600, 1025 |
| BstNSI | RCATGY | 3 cut(s) | 68, 413, 790 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 594 |
| BstSCI | CCNGG | 3 cut(s) | 517, 598, 1023 |
| BstSFI | CTRYAG | 1 cut(s) | 1347 |
| BstV1I | GCAGC | 3 cut(s) | 1007, 1181, 1228 |
| BstV2I | GAAGAC | 2 cut(s) | 66, 1310 |
| BstX2I | RGATCY | 1 cut(s) | 1252 |
| BstYI | RGATCY | 1 cut(s) | 1252 |
| BstZ17I | GTATAC | 1 cut(s) | 781 |
| Bsu36I | CCTNAGG | 1 cut(s) | 645 |
| BsuRI | GGCC | 3 cut(s) | 690, 702, 733 |
| BtgI | CCRYGG | 1 cut(s) | 692 |
| BtsCI | GGATG | 3 cut(s) | 264, 539, 1001 |
| BtsI | GCAGTG | 1 cut(s) | 666 |
| BtsIMutI | CAGTG | 3 cut(s) | 666, 718, 863 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1244 |
| CfoI | GCGC | 2 cut(s) | 1005, 1199 |
| Cfr13I | GGNCC | 1 cut(s) | 689 |
| CseI | GACGC | 1 cut(s) | 871 |
| Csp6I | GTAC | 8 cut(s) | 62, 256, 345, 455, 684, 790, 846, 1188 |
| CviAII | CATG | 5 cut(s) | 65, 410, 573, 693, 787 |
| CviQI | GTAC | 8 cut(s) | 62, 256, 345, 455, 684, 790, 846, 1188 |
| DdeI | CTNAG | 7 cut(s) | 78, 400, 458, 568, 645, 704, 954 |
| DpnI | GATC | 3 cut(s) | 855, 1215, 1254 |
| DpnII | GATC | 3 cut(s) | 853, 1213, 1252 |
| DraIII | CACNNNGTG | 1 cut(s) | 869 |
| Eco130I | CCWWGG | 2 cut(s) | 177, 692 |
| Eco147I | AGGCCT | 1 cut(s) | 733 |
| Eco31I | GGTCTC | 1 cut(s) | 993 |
| Eco32I | GATATC | 2 cut(s) | 1174, 1225 |
| Eco57I | CTGAAG | 2 cut(s) | 199, 1260 |
| Eco81I | CCTNAGG | 1 cut(s) | 645 |
| Eco88I | CYCGRG | 1 cut(s) | 230 |
| EcoRII | CCWGG | 3 cut(s) | 517, 598, 1023 |
| EcoRV | GATATC | 2 cut(s) | 1174, 1225 |
| EcoT14I | CCWWGG | 2 cut(s) | 177, 692 |
| ErhI | CCWWGG | 2 cut(s) | 177, 692 |
| Esp3I | CGTCTC | 1 cut(s) | 55 |
| FaeI | CATG | 5 cut(s) | 68, 413, 576, 696, 790 |
| FaqI | GGGAC | 3 cut(s) | 132, 363, 551 |
| FatI | CATG | 5 cut(s) | 64, 409, 572, 692, 786 |
| FauNDI | CATATG | 1 cut(s) | 652 |
| FblI | GTMKAC | 1 cut(s) | 780 |
| Fnu4HI | GCNGC | 3 cut(s) | 1021, 1195, 1242 |
| FokI | GGATG | 3 cut(s) | 251, 546, 1008 |
| Fsp4HI | GCNGC | 3 cut(s) | 1021, 1195, 1242 |
| FspBI | CTAG | 1 cut(s) | 992 |
| FspI | TGCGCA | 1 cut(s) | 1004 |
| GlaI | GCGC | 2 cut(s) | 1004, 1198 |
| GluI | GCNGC | 3 cut(s) | 1021, 1195, 1242 |
| GsaI | CCCAGC | 1 cut(s) | 1248 |
| HaeIII | GGCC | 3 cut(s) | 690, 702, 733 |
| HapII | CCGG | 1 cut(s) | 324 |
| HgaI | GACGC | 1 cut(s) | 871 |
| HhaI | GCGC | 2 cut(s) | 1005, 1199 |
| Hin1I | GRCGYC | 1 cut(s) | 882 |
| Hin1II | CATG | 5 cut(s) | 68, 413, 576, 696, 790 |
| Hin6I | GCGC | 2 cut(s) | 1003, 1197 |
| HinP1I | GCGC | 2 cut(s) | 1003, 1197 |
| HincII | GTYRAC | 1 cut(s) | 84 |
| HindII | GTYRAC | 1 cut(s) | 84 |
| HindIII | AAGCTT | 2 cut(s) | 196, 487 |
| HinfI | GANTC | 8 cut(s) | 235, 317, 440, 708, 806, 919, 945, 1108 |
| HpaII | CCGG | 1 cut(s) | 324 |
| HphI | GGTGA | 4 cut(s) | 711, 926, 1105, 1223 |
| Hpy166II | GTNNAC | 4 cut(s) | 84, 719, 781, 1096 |
| Hpy188I | TCNGA | 7 cut(s) | 372, 505, 586, 660, 1171, 1178, 1279 |
| Hpy188III | TCNNGA | 7 cut(s) | 221, 230, 331, 644, 819, 851, 962 |
| Hpy8I | GTNNAC | 4 cut(s) | 84, 719, 781, 1096 |
| HpyAV | CCTTC | 3 cut(s) | 64, 510, 806 |
| HpyCH4III | ACNGT | 6 cut(s) | 61, 280, 344, 427, 867, 1145 |
| HpyCH4IV | ACGT | 2 cut(s) | 509, 631 |
| HpyCH4V | TGCA | 9 cut(s) | 4, 68, 293, 303, 422, 467, 671, 1122, 1322 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 410, 419 |
| HpyF3I | CTNAG | 7 cut(s) | 78, 400, 458, 568, 645, 704, 954 |
| HpySE526I | ACGT | 2 cut(s) | 509, 631 |
| Hsp92I | GRCGYC | 1 cut(s) | 882 |
| Hsp92II | CATG | 5 cut(s) | 68, 413, 576, 696, 790 |
| HspAI | GCGC | 2 cut(s) | 1003, 1197 |
| Kzo9I | GATC | 3 cut(s) | 853, 1213, 1252 |
| LmnI | GCTCC | 1 cut(s) | 890 |
| Lsp1109I | GCAGC | 3 cut(s) | 1007, 1181, 1228 |
| LweI | GCATC | 3 cut(s) | 290, 336, 454 |
| MaeI | CTAG | 1 cut(s) | 992 |
| MaeII | ACGT | 2 cut(s) | 509, 631 |
| MaeIII | GTNAC | 2 cut(s) | 239, 513 |
| MalI | GATC | 3 cut(s) | 855, 1215, 1254 |
| MboI | GATC | 3 cut(s) | 853, 1213, 1252 |
| MboII | GAAGA | 5 cut(s) | 66, 205, 312, 538, 1315 |
| MfeI | CAATTG | 1 cut(s) | 42 |
| MflI | RGATCY | 1 cut(s) | 1252 |
| MlyI | GAGTC | 1 cut(s) | 815 |
| MnlI | CCTC | 7 cut(s) | 160, 537, 640, 766, 1172, 1175, 1178 |
| MseI | TTAA | 4 cut(s) | 117, 312, 728, 1016 |
| MslI | CAYNNNNRTG | 2 cut(s) | 95, 332 |
| MspA1I | CMGCKG | 1 cut(s) | 1241 |
| MspI | CCGG | 1 cut(s) | 324 |
| MspR9I | CCNGG | 3 cut(s) | 519, 600, 1025 |
| MunI | CAATTG | 1 cut(s) | 42 |
| Mva1269I | GAATGC | 1 cut(s) | 1124 |
| MvaI | CCWGG | 3 cut(s) | 519, 600, 1025 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 410, 419 |
| NcoI | CCATGG | 1 cut(s) | 692 |
| NdeI | CATATG | 1 cut(s) | 652 |
| NdeII | GATC | 3 cut(s) | 853, 1213, 1252 |
| NlaIII | CATG | 5 cut(s) | 68, 413, 576, 696, 790 |
| NlaIV | GGNNCC | 3 cut(s) | 130, 472, 892 |
| NsbI | TGCGCA | 1 cut(s) | 1004 |
| NspI | RCATGY | 3 cut(s) | 68, 413, 790 |
| NspV | TTCGAA | 1 cut(s) | 1264 |
| OliI | CACNNNNGTG | 1 cut(s) | 95 |
| PceI | AGGCCT | 1 cut(s) | 733 |
| PciI | ACATGT | 1 cut(s) | 786 |
| PctI | GAATGC | 1 cut(s) | 1124 |
| PfeI | GAWTC | 7 cut(s) | 235, 317, 440, 708, 919, 945, 1108 |
| PflFI | GACNNNGTC | 1 cut(s) | 758 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 698, 1244 |
| PfoI | TCCNGGA | 1 cut(s) | 598 |
| PkrI | GCNGC | 3 cut(s) | 1022, 1196, 1243 |
| PleI | GAGTC | 1 cut(s) | 814 |
| PpsI | GAGTC | 1 cut(s) | 814 |
| PscI | ACATGT | 1 cut(s) | 786 |
| PshAI | GACNNNNGTC | 1 cut(s) | 594 |
| PshBI | ATTAAT | 1 cut(s) | 312 |
| Psp6I | CCWGG | 3 cut(s) | 517, 598, 1023 |
| PspFI | CCCAGC | 1 cut(s) | 1244 |
| PspGI | CCWGG | 3 cut(s) | 517, 598, 1023 |
| PspN4I | GGNNCC | 3 cut(s) | 130, 472, 892 |
| PspPI | GGNCC | 1 cut(s) | 689 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1244 |
| PsuI | RGATCY | 1 cut(s) | 1252 |
| PsyI | GACNNNGTC | 1 cut(s) | 758 |
| PvuII | CAGCTG | 1 cut(s) | 1241 |
| RsaI | GTAC | 8 cut(s) | 63, 257, 346, 456, 685, 791, 847, 1189 |
| RsaNI | GTAC | 8 cut(s) | 62, 256, 345, 455, 684, 790, 846, 1188 |
| RseI | CAYNNNNRTG | 2 cut(s) | 95, 332 |
| SaqAI | TTAA | 4 cut(s) | 117, 312, 728, 1016 |
| SatI | GCNGC | 3 cut(s) | 1021, 1195, 1242 |
| Sau3AI | GATC | 3 cut(s) | 853, 1213, 1252 |
| Sau96I | GGNCC | 1 cut(s) | 689 |
| ScaI | AGTACT | 2 cut(s) | 456, 847 |
| SchI | GAGTC | 1 cut(s) | 815 |
| ScrFI | CCNGG | 3 cut(s) | 519, 600, 1025 |
| SfaNI | GCATC | 3 cut(s) | 290, 336, 454 |
| SfcI | CTRYAG | 1 cut(s) | 1347 |
| SfuI | TTCGAA | 1 cut(s) | 1264 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 95, 332 |
| SmlI | CTYRAG | 4 cut(s) | 541, 817, 960, 1112 |
| SmoI | CTYRAG | 4 cut(s) | 541, 817, 960, 1112 |
| SseBI | AGGCCT | 1 cut(s) | 733 |
| SsiI | CCGC | 2 cut(s) | 25, 888 |
| SspMI | CTAG | 1 cut(s) | 992 |
| StuI | AGGCCT | 1 cut(s) | 733 |
| StyD4I | CCNGG | 3 cut(s) | 517, 598, 1023 |
| StyI | CCWWGG | 2 cut(s) | 177, 692 |
| TaaI | ACNGT | 6 cut(s) | 61, 280, 344, 427, 867, 1145 |
| TaiI | ACGT | 2 cut(s) | 512, 634 |
| TaqI | TCGA | 2 cut(s) | 753, 1264 |
| TatI | WGTACW | 3 cut(s) | 454, 789, 845 |
| TfiI | GAWTC | 7 cut(s) | 235, 317, 440, 708, 919, 945, 1108 |
| Tru1I | TTAA | 4 cut(s) | 117, 312, 728, 1016 |
| Tru9I | TTAA | 4 cut(s) | 117, 312, 728, 1016 |
| TscAI | CASTG | 3 cut(s) | 673, 718, 870 |
| TseI | GCWGC | 3 cut(s) | 1020, 1194, 1241 |
| TspDTI | ATGAA | 2 cut(s) | 539, 1121 |
| TspGWI | ACGGA | 3 cut(s) | 152, 574, 1318 |
| TspRI | CASTG | 3 cut(s) | 673, 718, 870 |
| Tth111I | GACNNNGTC | 1 cut(s) | 758 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 698, 1244 |
| VspI | ATTAAT | 1 cut(s) | 312 |
| XapI | RAATTY | 5 cut(s) | 249, 448, 580, 973, 1052 |
| XceI | RCATGY | 3 cut(s) | 68, 413, 790 |
| XmiI | GTMKAC | 1 cut(s) | 780 |
| XspI | CTAG | 1 cut(s) | 992 |
| ZrmI | AGTACT | 2 cut(s) | 456, 847 |