MD02G1167100.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000207
Physical Location & Seq
Reverse (-)
14349389 .. 14353191
3803 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1167100.v1.1.491

Sequence Viewer

Length: 2487 bp
ATGTACAGTTTCAGAAATATGCCAATGCAGTCCTTGAGGAGCATATTTGTGTGTTTGTTCTTATTATCCTTCCTAAGGATCTCATCTGCAGCTTCACTAGCCACAATCACTCCGAGTCGATCTATTAGAGATGGCGAAACTCTGGTTTCAGCAGGTGGAGGCTTTGAACTGGGATTTTTCAGCCCTGGTAACTCGAAGGGCCGGTACTTGGGGATATGGTACACAGTTTCTGCTGATACAGTTGTGTGGGTAGCCAACAGAGAAACACCACTTGGTGATTCGTCCGGATTTTTGAAGCTCACCGAGCAAGGAGTACTAGTCCTCCTTAATAGCTCAGACAACATTGTATGGTCATCGAATTCATCAAGAACCGTGGGGAATCCAGTATCACAACTCTTGGATACTGGAAATCTTATTGTGAAAGACGGAAATGAAACTAACTCGGATAGCTTCTCGTGGCAGAGTTTTGATTACCCTTGTGACACATTCCTACCGGAAATGAAGCTTGGGTGGGACTTAGCTAATGGTTTAGAGAGGTATGCCTCGTCTTGGAAGAGCACAGAAAATCCTGCTCCAGGAGATTTTTCTCTACGGTTAAGTCATTGGGGCTTACCACAACTTGTTACTATGAAAGGAGCTAAGATACAGGCTAGAGGAGGGTCATGGAATGGTGTTCATCTTACAGGATATGGAATTAGTGGAAGAAAATCCAATCCAATATCTGGGCTTGAATTTCGGTTGAACAAGGATGAGGTCTACTTTGAGTACAGGCTCCTAAACAGGTCGGAGTTCACGAGATACGTATTGAACCCGTTAGGCATCGCACAACGGTTCACATGGGTGCGCCAAACACAGAGTTGGGAACTTTCCTCTACATTCCAAGCAGATCGGTGTTCAAACTATGCCTTGTGTGGTGCATATACTACTTGCAATATCAACAACGCTCTAATATGTGCATGCTTGAAGGGATTTACACCAAAATCTCCAAAAGATTGGGACTCGGGATACTGGTCCGACGGGTGCGTTCGAAAGACTCCGTTGGCCTGCAGCCATGGAGATGGCTTCCTAAAGTATAACGGGATTAAATTGCCAGACACATCTACCTCATGGTTTAATAAAAGCATGAGCCTCAAGGAATGCAAGTGGTTATGTTTGGAAAACTGCTCATGTACGGCGTATGCAAATCTTGATATCAGGGAGGGAGGAAGTGGCTGCTTGCTTTGGTTTGGCAACCTCATTGACATAAGAGAATTCGCTATAAGTGACGTTCAGGACTTCTATATACGGATGGCAGCTTCAGAACTAGATCACTTTGTGAAAGAGAGCAAGTTCAACGAGAAAAAACTACCAATTCTCATCAGCTCCGCGGTTTTACTCCTCGGAATGATTATAGTTGGATTGATATTGTACATACGGAAGAAGAAACTCAGAAATGAAGGAGTCAGAAGAAAGGATTTCAGAAAAGAGTACCTTGGAGAAGACAGGGAAGGCATGGAGTTACCGCTATTTGACTTGACCACCATAGTCAAAGCGACTGATAACTTTTCAAGCAACAAGTTGGGAGAAGGTGGTTTTGGACCTGTGTACAAGGGCACATTGATGGGAGGGAAAGAAGTTGCAGTTAAGAGACTTTCAAAGGATTCTGGACAGGGAATGAGGGAGTTCAAAAATGAAGTAATACTGATAGCCAGACTTCAGCATCGGAATCTCGTAAAGCTTATGGGCTATTGCACTCAAGAAGATGAAAAGATTCTAATATATGAATTCATGCCAAATGGAAGCTTAGACTTCTTTATTTTTGATGAAGAGGGAGGAAAGTTGCTCAACTGGCCTAAGTGCTTTCACATTATTGGTGGAATTGCTCGGGGACTTGTTTATCTTCACCATGACTCTAGATTAAGGATTATCCACCGAGATCTGAAAGCTAGCAATATTCTGCTTGATAATAATATGAACCCGAAGATTGCAGACTTTGGCTTGGCTAAAATATTCGACACAGATCAAAGTCAGGCCAATACAAAGAGAGTGGTAGGAACCTATGGTTATATGTCTCCCGAATATGCTATAGATGGAATTTGCTCGATGAAATCTGATGTCTTTAGCTTTGGAGTTATACTGCTAGAGATGTTGAGTAGGAAGAAGAACAGGGGATTTTGTCATCCGGATCATCACCTTAACCTTCTTGGACATGCATGGATGCTATGGATGCAAGATAAACAACTGGAACTGATCGATAAGACTCTTTCTGATTCGTGCAATATATGTGAAGTGGTAAGGTGTCTTCACGTGGGGCTGTTATGTGTGCAAAGAGTACCTGAGGATAGACCAAGCATGTCATCTGTGGTTCTGATGTTGAGCAGTGATGTTGCCTTGCCTTCTCCAAAGCAGCCTGGTTTTTACACTGAACGAAGTGTCACTGAATCACCATCAAGCATGCGTCCGTGTTCAGAGAATAATGTCAGCACTACATTGATAGAACCTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

829

Amino Acids

92.97

Weight (kDa)

8.29

Isoelectric Point (pI)

42.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 80 - 185 2.8e-37 D-mannose binding lectin
S_locus_glycop PF00954 218 - 328 4.8e-23 S-locus glycoprotein domain
PAN_2 PF08276 349 - 415 1.7e-22 PAN-like domain
PK_Tyr_Ser-Thr PF07714 518 - 782 1.4e-46 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 518 - 715 2.8e-44 Protein kinase domain
DUF3403 PF11883 786 - 828 1.4e-09 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27300
fragaria_vesca FvH4_3g15930 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g21350 FvH4_3g21400 FvH4_3g21400 FvH4_6g12332
malus_domestica MD02G1167100.v1.1 MD02G1167200.v1.1 MD03G1185600.v1.1 MD05G1213900.v1.1 MD05G1214100.v1.1 MD05G1214200.v1.1 MD05G1214700.v1.1 MD05G1216300.v1.1 MD05G1216800.v1.1 MD05G1217300.v1.1 MD05G1218000.v1.1 MD11G1231100.v1.1 MD11G1231200.v1.1 MD11G1231400.v1.1 MD11G1231500.v1.1 MD11G1232400.v1.1 MD11G1232500.v1.1
prunus_persica Prupe.4G142300_v2.0.a1 Prupe.4G142400_v2.0.a1 Prupe.4G142500_v2.0.a1 Prupe.4G142600_v2.0.a1 Prupe.4G142800_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195100_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195500_v2.0.a1 Prupe.4G195700_v2.0.a1 Prupe.4G195900_v2.0.a1
pyrus_communis pycom03g16540 pycom05g19920 pycom11g20460 pycom11g20480 pycom11g20490 pycom11g20500 pycom11g20530
rosa_chinensis RchiOBHm_Chr2g0139311 RchiOBHm_Chr2g0139351 RchiOBHm_Chr5g0026801 RchiOBHm_Chr5g0026851 RchiOBHm_Chr5g0026891 RchiOBHm_Chr5g0026911 RchiOBHm_Chr5g0036571 RchiOBHm_Chr5g0036591 RchiOBHm_Chr5g0036721 RchiOBHm_Chr5g0036741 RchiOBHm_Chr7g0222981
rosa_laevigata RLG00000002006 RLG00000019807 RLG00000032964 RLG00000032968 RLG00000032969 RLG00000033706 RLG00000033708 RLG00000033712 RLG00000033716
rosa_multiflora Rmu_co8256651.1_g000001 Rmu_co8279377.1_g000001 Rmu_co8429919.1_g000001 Rmu_co8490103.1_g000001 Rmu_sc0000774.1_g000002 Rmu_sc0002525.1_g000012 Rmu_sc0004223.1_g000008 Rmu_sc0004390.1_g000002 Rmu_sc0006059.1_g000014 Rmu_sc0006059.1_g000024 Rmu_sc0006514.1_g000020 Rmu_sc0014333.1_g000002 Rmu_ssc0000172.1_g000018 Rmu_ssc0000172.1_g000029
rosa_roxburghii Rroxscaffold_1G00044230 Rroxscaffold_1G00044260 Rroxscaffold_1G00044320 Rroxscaffold_1G00044340 Rroxscaffold_1G00052560 Rroxscaffold_1G00052570 Rroxscaffold_1G00052660 Rroxscaffold_1G00052720 Rroxscaffold_2G00105450 Rroxscaffold_2G00105460
rosa_rugosa Rorug02G0356900 Rorug02G0357000 Rorug02G0357100 Rorug02G0357200 Rorug02G0357300 Rorug02G0357400 Rorug02G0357500 Rorug02G0357600 Rorug05G0095400 Rorug05G0095500 Rorug05G0095500 Rorug05G0095600 Rorug05G0095700 Rorug05G0095800 Rorug05G0157700 Rorug05G0157800
rosa_samantha Rh2AG406800 Rh2AG406900 Rh2BG417300 Rh2CG392900 Rh2DG426200 Rh5AG189300 Rh5AG249400 Rh5AG250100 Rh5BG186300 Rh5BG186900 Rh5BG187000 Rh5BG249500 Rh5BG250600 Rh5BG250900 Rh5BG251500 Rh5BG251800 Rh5CG205500 Rh5CG206300 Rh5CG206700 Rh5CG283000 Rh5CG283300 Rh5CG283500 Rh5DG188500 Rh5DG188700 Rh5DG258700 Rh5DG258900 Rh5DG259600 Rh5DG259900 Rh7CG375800 Rh7DG352500
rosa_wichuraiana Rw2G032960 Rw2G032970 Rw2G033220 Rw2G033230 Rw5G017130 Rw5G017230 Rw5G022940 Rw5G022960 Rw5G022980 Rw7G030420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 143
Acc36I ACCTGC 1 cut(s) 143
AccB7I CCANNNNNTGG 1 cut(s) 722
AccI GTMKAC 1 cut(s) 756
AccII CGCG 1 cut(s) 1367
AccIII TCCGGA 2 cut(s) 284, 2161
AciI CCGC 3 cut(s) 1365, 1367, 1502
AclWI GGATC 2 cut(s) 86, 2172
AcsI RAATTY 5 cut(s) 358, 731, 1250, 1763, 2073
AcuI CTGAAG 2 cut(s) 1281, 1679
AcvI CACGTG 1 cut(s) 2287
AdeI CACNNNGTG 2 cut(s) 275, 1315
AfiI CCNNNNNNNGG 5 cut(s) 75, 208, 549, 575, 722
AhlI ACTAGT 1 cut(s) 316
AjnI CCWGG 3 cut(s) 184, 574, 2389
AjuI GAANNNNNNNTTGG 6 cut(s) 255, 287, 489, 521, 1557, 1589
AleI CACNNNNGTG 1 cut(s) 839
Alw21I GWGCWC 1 cut(s) 560
Alw26I GTCTC 2 cut(s) 1621, 2055
AlwI GGATC 2 cut(s) 86, 2172
AlwNI CAGNNNCTG 1 cut(s) 230
Ama87I CYCGRG 2 cut(s) 1000, 1863
Aor13HI TCCGGA 2 cut(s) 284, 2161
AoxI GGCC 4 cut(s) 199, 1041, 1829, 2010
ApeKI GCWGC 5 cut(s) 89, 1047, 1212, 1292, 2386
ApoI RAATTY 5 cut(s) 358, 731, 1250, 1763, 2073
Asp700I GAANNNNTTC 1 cut(s) 1749
AspLEI GCGC 1 cut(s) 846
AspS9I GGNCC 3 cut(s) 199, 1011, 1577
AsuHPI GGTGA 5 cut(s) 287, 292, 1874, 2162, 2415
AsuII TTCGAA 1 cut(s) 1027
AsuNHI GCTAGC 1 cut(s) 1925
AvaI CYCGRG 2 cut(s) 1000, 1863
AvaII GGWCC 2 cut(s) 1011, 1577
AxyI CCTNAGG 2 cut(s) 74, 2316
BaeGI GKGCMC 1 cut(s) 1595
BaeI ACNNNNGTAYC 2 cut(s) 228, 261
BarI GAAGNNNNNNTAC 4 cut(s) 188, 220, 2265, 2297
BauI CACGAG 2 cut(s) 454, 793
BbrPI CACGTG 1 cut(s) 2287
BbsI GAAGAC 2 cut(s) 1485, 2273
Bbv12I GWGCWC 1 cut(s) 560
BbvI GCAGC 5 cut(s) 101, 1059, 1199, 1304, 2398
BccI CCATC 6 cut(s) 125, 1052, 1282, 1594, 2063, 2434
BceAI ACGGC 1 cut(s) 1188
BciT130I CCWGG 3 cut(s) 186, 576, 2391
BciVI GTATCC 2 cut(s) 394, 998
BcoDI GTCTC 2 cut(s) 1621, 2055
BcuI ACTAGT 1 cut(s) 316
BfaI CTAG 7 cut(s) 98, 317, 651, 1304, 1893, 1926, 2120
BfmI CTRYAG 3 cut(s) 87, 1045, 2064
BfuAI ACCTGC 1 cut(s) 143
BfuI GTATCC 2 cut(s) 394, 998
BglII AGATCT 1 cut(s) 1915
BisI GCNGC 5 cut(s) 90, 1048, 1213, 1293, 2387
BlsI GCNGC 5 cut(s) 91, 1049, 1214, 1294, 2388
BmcAI AGTACT 1 cut(s) 315
Bme1390I CCNGG 3 cut(s) 186, 576, 2391
Bme18I GGWCC 2 cut(s) 1011, 1577
BmeT110I CYCGRG 2 cut(s) 1000, 1863
BmgT120I GGNCC 3 cut(s) 199, 1011, 1577
BmiI GGNNCC 2 cut(s) 773, 2035
BmrFI CCNGG 3 cut(s) 186, 576, 2391
BmrI ACTGGG 1 cut(s) 179
BmsI GCATC 4 cut(s) 828, 1708, 2187, 2196
BmtI GCTAGC 1 cut(s) 1929
BmuI ACTGGG 1 cut(s) 179
BpiI GAAGAC 2 cut(s) 1485, 2273
BpmI CTGGAG 1 cut(s) 558
Bpu14I TTCGAA 1 cut(s) 1027
BpuEI CTTGAG 3 cut(s) 55, 1115, 1719
Bsa29I ATCGAT 1 cut(s) 2232
BsaAI YACGTR 2 cut(s) 802, 2287
BsaJI CCNNGG 7 cut(s) 184, 372, 1051, 1365, 1378, 1471, 2480
BsaWI WCCGGW 3 cut(s) 284, 493, 2161
BsaXI ACNNNNNCTCC 6 cut(s) 94, 124, 303, 306, 333, 336
Bsc4I CCNNNNNNNGG 5 cut(s) 75, 208, 549, 575, 722
Bse118I RCCGGY 1 cut(s) 201
Bse1I ACTGG 6 cut(s) 174, 383, 409, 1013, 1832, 2226
Bse21I CCTNAGG 2 cut(s) 74, 2316
BseAI TCCGGA 2 cut(s) 284, 2161
BseBI CCWGG 3 cut(s) 186, 576, 2391
BseCI ATCGAT 1 cut(s) 2232
BseDI CCNNGG 7 cut(s) 184, 372, 1051, 1365, 1378, 1471, 2480
BseGI GGATG 5 cut(s) 754, 1293, 2158, 2202, 2211
BseLI CCNNNNNNNGG 5 cut(s) 75, 208, 549, 575, 722
BseMII CTCAG 3 cut(s) 348, 1441, 2307
BseNI ACTGG 6 cut(s) 174, 383, 409, 1013, 1832, 2226
BseRI GAGGAG 3 cut(s) 52, 669, 1367
BseSI GKGCMC 1 cut(s) 1595
BseXI GCAGC 5 cut(s) 101, 1059, 1199, 1304, 2398
Bsh1236I CGCG 1 cut(s) 1367
BshFI GGCC 4 cut(s) 201, 1043, 1831, 2012
BshVI ATCGAT 1 cut(s) 2232
BsiHKAI GWGCWC 1 cut(s) 560
BsiHKCI CYCGRG 2 cut(s) 1000, 1863
BsiSI CCGG 4 cut(s) 202, 285, 494, 2162
BslFI GGGAC 3 cut(s) 527, 1010, 1881
BslI CCNNNNNNNGG 5 cut(s) 75, 208, 549, 575, 722
BsmAI GTCTC 2 cut(s) 1621, 2055
BsmFI GGGAC 3 cut(s) 527, 1010, 1881
BsmI GAATGC 1 cut(s) 1142
BsnI GGCC 4 cut(s) 201, 1043, 1831, 2012
BsoBI CYCGRG 2 cut(s) 1000, 1863
Bsp119I TTCGAA 1 cut(s) 1027
Bsp1286I GDGCHC 2 cut(s) 560, 1595
Bsp13I TCCGGA 2 cut(s) 284, 2161
Bsp1407I TGTACA 3 cut(s) 3, 1407, 1584
Bsp143I GATC 8 cut(s) 78, 119, 886, 1306, 1915, 1999, 2164, 2229
Bsp19I CCATGG 1 cut(s) 1051
BspACI CCGC 3 cut(s) 1365, 1367, 1502
BspANI GGCC 4 cut(s) 201, 1043, 1831, 2012
BspCNI CTCAG 3 cut(s) 347, 1440, 2308
BspDI ATCGAT 1 cut(s) 2232
BspEI TCCGGA 2 cut(s) 284, 2161
BspFNI CGCG 1 cut(s) 1367
BspLI GGNNCC 2 cut(s) 773, 2035
BspMAI CTGCAG 2 cut(s) 91, 1049
BspMI ACCTGC 1 cut(s) 143
BspOI GCTAGC 1 cut(s) 1929
BspPI GGATC 2 cut(s) 86, 2172
BspQI GCTCTTC 1 cut(s) 548
BspT104I TTCGAA 1 cut(s) 1027
BsrFI RCCGGY 1 cut(s) 201
BsrGI TGTACA 3 cut(s) 3, 1407, 1584
BsrI ACTGG 6 cut(s) 174, 383, 409, 1013, 1832, 2226
BssAI RCCGGY 1 cut(s) 201
BssECI CCNNGG 7 cut(s) 184, 372, 1051, 1365, 1378, 1471, 2480
BssMI GATC 8 cut(s) 78, 119, 886, 1306, 1915, 1999, 2164, 2229
BssSI CACGAG 2 cut(s) 454, 793
BssT1I CCWWGG 2 cut(s) 1051, 1471
Bst2BI CACGAG 2 cut(s) 454, 793
Bst2UI CCWGG 3 cut(s) 186, 576, 2391
Bst4CI ACNGT 6 cut(s) 8, 226, 241, 373, 594, 831
Bst6I CTCTTC 2 cut(s) 548, 1800
BstAUI TGTACA 3 cut(s) 3, 1407, 1584
BstBAI YACGTR 2 cut(s) 802, 2287
BstBI TTCGAA 1 cut(s) 1027
BstC8I GCNNGC 5 cut(s) 958, 1045, 1217, 1927, 2435
BstDEI CTNAG 8 cut(s) 74, 334, 517, 639, 1427, 1783, 1833, 2316
BstDSI CCRYGG 3 cut(s) 372, 1051, 1365
BstENI CCTNNNNNAGG 2 cut(s) 73, 573
BstF5I GGATG 5 cut(s) 754, 1293, 2158, 2202, 2211
BstFNI CGCG 1 cut(s) 1367
BstHHI GCGC 1 cut(s) 846
BstKTI GATC 8 cut(s) 81, 122, 889, 1309, 1918, 2002, 2167, 2232
BstMAI GTCTC 2 cut(s) 1621, 2055
BstMBI GATC 8 cut(s) 78, 119, 886, 1306, 1915, 1999, 2164, 2229
BstMWI GCNNNNNNNGC 4 cut(s) 98, 304, 1828, 2206
BstNI CCWGG 3 cut(s) 186, 576, 2391
BstNSI RCATGY 4 cut(s) 960, 2192, 2335, 2437
BstSCI CCNGG 3 cut(s) 184, 574, 2389
BstSFI CTRYAG 3 cut(s) 87, 1045, 2064
BstSLI GKGCMC 1 cut(s) 1595
BstSNI TACGTA 1 cut(s) 802
BstUI CGCG 1 cut(s) 1367
BstV1I GCAGC 5 cut(s) 101, 1059, 1199, 1304, 2398
BstV2I GAAGAC 2 cut(s) 1485, 2273
BstX2I RGATCY 2 cut(s) 78, 1915
BstXI CCANNNNNNTGG 2 cut(s) 993, 1058
BstYI RGATCY 2 cut(s) 78, 1915
Bsu15I ATCGAT 1 cut(s) 2232
Bsu36I CCTNAGG 2 cut(s) 74, 2316
BsuI GTATCC 2 cut(s) 394, 998
BsuRI GGCC 4 cut(s) 201, 1043, 1831, 2012
BsuTUI ATCGAT 1 cut(s) 2232
BtgI CCRYGG 3 cut(s) 372, 1051, 1365
BtgZI GCGATG 1 cut(s) 805
BtsCI GGATG 5 cut(s) 754, 1293, 2158, 2202, 2211
BtsI GCAGTG 1 cut(s) 2365
BtsIMutI CAGTG 3 cut(s) 2365, 2400, 2415
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 5 cut(s) 958, 1045, 1217, 1927, 2435
CaiI CAGNNNCTG 1 cut(s) 230
CfoI GCGC 1 cut(s) 846
Cfr10I RCCGGY 1 cut(s) 201
Cfr13I GGNCC 3 cut(s) 199, 1011, 1577
Cfr42I CCGCGG 1 cut(s) 1368
ClaI ATCGAT 1 cut(s) 2232
CseI GACGC 1 cut(s) 2426
CspCI CAANNNNNGTGG 4 cut(s) 354, 389, 2007, 2042
DdeI CTNAG 8 cut(s) 74, 334, 517, 639, 1427, 1783, 1833, 2316
DpnI GATC 8 cut(s) 80, 121, 888, 1308, 1917, 2001, 2166, 2231
DpnII GATC 8 cut(s) 78, 119, 886, 1306, 1915, 1999, 2164, 2229
DraIII CACNNNGTG 2 cut(s) 275, 1315
Eam1104I CTCTTC 2 cut(s) 548, 1800
EarI CTCTTC 2 cut(s) 548, 1800
Eco105I TACGTA 1 cut(s) 802
Eco130I CCWWGG 2 cut(s) 1051, 1471
Eco32I GATATC 1 cut(s) 1192
Eco47I GGWCC 2 cut(s) 1011, 1577
Eco57I CTGAAG 2 cut(s) 1281, 1679
Eco72I CACGTG 1 cut(s) 2287
Eco81I CCTNAGG 2 cut(s) 74, 2316
Eco88I CYCGRG 2 cut(s) 1000, 1863
EcoNI CCTNNNNNAGG 2 cut(s) 73, 573
EcoRI GAATTC 3 cut(s) 358, 1250, 1763
EcoRII CCWGG 3 cut(s) 184, 574, 2389
EcoRV GATATC 1 cut(s) 1192
EcoT14I CCWWGG 2 cut(s) 1051, 1471
EcoT22I ATGCAT 1 cut(s) 2194
ErhI CCWWGG 2 cut(s) 1051, 1471
FalI AAGNNNNNCTT 2 cut(s) 1455, 1487
FaqI GGGAC 3 cut(s) 527, 1010, 1881
FblI GTMKAC 1 cut(s) 756
Fnu4HI GCNGC 5 cut(s) 90, 1048, 1213, 1293, 2387
FokI GGATG 5 cut(s) 761, 1300, 2145, 2209, 2218
Fsp4HI GCNGC 5 cut(s) 90, 1048, 1213, 1293, 2387
FspBI CTAG 7 cut(s) 98, 317, 651, 1304, 1893, 1926, 2120
GlaI GCGC 1 cut(s) 845
GluI GCNGC 5 cut(s) 90, 1048, 1213, 1293, 2387
GsuI CTGGAG 1 cut(s) 558
HaeIII GGCC 4 cut(s) 201, 1043, 1831, 2012
HapII CCGG 4 cut(s) 202, 285, 494, 2162
HgaI GACGC 1 cut(s) 2426
HhaI GCGC 1 cut(s) 846
Hin6I GCGC 1 cut(s) 844
HinP1I GCGC 1 cut(s) 844
HindIII AAGCTT 3 cut(s) 503, 1715, 1780
HpaII CCGG 4 cut(s) 202, 285, 494, 2162
HphI GGTGA 5 cut(s) 287, 292, 1874, 2162, 2415
Hpy166II GTNNAC 5 cut(s) 222, 757, 792, 834, 1585
Hpy8I GTNNAC 5 cut(s) 222, 757, 792, 834, 1585
Hpy99I CGWCG 1 cut(s) 1019
HpyAV CCTTC 8 cut(s) 79, 190, 958, 1430, 1481, 1559, 2189, 2385
HpyCH4III ACNGT 6 cut(s) 8, 226, 241, 373, 594, 831
HpyCH4IV ACGT 3 cut(s) 801, 1266, 2286
HpyF10VI GCNNNNNNNGC 4 cut(s) 98, 304, 1828, 2206
HpyF3I CTNAG 8 cut(s) 74, 334, 517, 639, 1427, 1783, 1833, 2316
HpySE526I ACGT 3 cut(s) 801, 1266, 2286
HspAI GCGC 1 cut(s) 844
Kpn2I TCCGGA 2 cut(s) 284, 2161
KspI CCGCGG 1 cut(s) 1368
Kzo9I GATC 8 cut(s) 78, 119, 886, 1306, 1915, 1999, 2164, 2229
LguI GCTCTTC 1 cut(s) 548
LmnI GCTCC 5 cut(s) 39, 577, 635, 777, 1367
Lsp1109I GCAGC 5 cut(s) 101, 1059, 1199, 1304, 2398
LweI GCATC 4 cut(s) 828, 1708, 2187, 2196
MaeI CTAG 7 cut(s) 98, 317, 651, 1304, 1893, 1926, 2120
MaeII ACGT 3 cut(s) 801, 1266, 2286
MaeIII GTNAC 6 cut(s) 188, 479, 622, 1262, 1497, 2413
MalI GATC 8 cut(s) 80, 121, 888, 1308, 1917, 2001, 2166, 2231
MboI GATC 8 cut(s) 78, 119, 886, 1306, 1915, 1999, 2164, 2229
MflI RGATCY 2 cut(s) 78, 1915
MhlI GDGCHC 2 cut(s) 560, 1595
MluCI AATT 9 cut(s) 358, 693, 731, 1085, 1250, 1350, 1763, 1857, 2073
MlyI GAGTC 6 cut(s) 124, 992, 1027, 1449, 1883, 2233
MmeI TCCRAC 3 cut(s) 765, 1038, 1375
Mph1103I ATGCAT 1 cut(s) 2194
MroI TCCGGA 2 cut(s) 284, 2161
MroXI GAANNNNTTC 1 cut(s) 1749
MseI TTAA 7 cut(s) 327, 596, 1083, 1113, 1623, 1898, 2175
MslI CAYNNNNRTG 4 cut(s) 47, 839, 1056, 1598
MspA1I CMGCKG 1 cut(s) 1367
MspI CCGG 4 cut(s) 202, 285, 494, 2162
MspR9I CCNGG 3 cut(s) 186, 576, 2391
Mva1269I GAATGC 1 cut(s) 1142
MvaI CCWGG 3 cut(s) 186, 576, 2391
MvnI CGCG 1 cut(s) 1367
MwoI GCNNNNNNNGC 4 cut(s) 98, 304, 1828, 2206
NcoI CCATGG 1 cut(s) 1051
NdeII GATC 8 cut(s) 78, 119, 886, 1306, 1915, 1999, 2164, 2229
NheI GCTAGC 1 cut(s) 1925
NlaIV GGNNCC 2 cut(s) 773, 2035
NmuCI GTSAC 3 cut(s) 479, 1262, 2413
NsiI ATGCAT 1 cut(s) 2194
NspI RCATGY 4 cut(s) 960, 2192, 2335, 2437
NspV TTCGAA 1 cut(s) 1027
OliI CACNNNNGTG 1 cut(s) 839
PaeI GCATGC 2 cut(s) 960, 2437
PaqCI CACCTGC 1 cut(s) 143
PciSI GCTCTTC 1 cut(s) 548
PcsI WCGNNNNNNNCGW 1 cut(s) 791
PctI GAATGC 1 cut(s) 1142
PdmI GAANNNNTTC 1 cut(s) 1749
PfeI GAWTC 7 cut(s) 278, 379, 1640, 1705, 1750, 2249, 2420
PflMI CCANNNNNTGG 1 cut(s) 722
PfoI TCCNGGA 1 cut(s) 574
PkrI GCNGC 5 cut(s) 91, 1049, 1214, 1294, 2388
PleI GAGTC 6 cut(s) 123, 992, 1027, 1448, 1883, 2233
PmaCI CACGTG 1 cut(s) 2287
PmlI CACGTG 1 cut(s) 2287
PpsI GAGTC 6 cut(s) 123, 992, 1027, 1448, 1883, 2233
Ppu21I YACGTR 2 cut(s) 802, 2287
Psp6I CCWGG 3 cut(s) 184, 574, 2389
PspCI CACGTG 1 cut(s) 2287
PspGI CCWGG 3 cut(s) 184, 574, 2389
PspN4I GGNNCC 2 cut(s) 773, 2035
PspPI GGNCC 3 cut(s) 199, 1011, 1577
PstI CTGCAG 2 cut(s) 91, 1049
PstNI CAGNNNCTG 1 cut(s) 230
PsuI RGATCY 2 cut(s) 78, 1915
RseI CAYNNNNRTG 4 cut(s) 47, 839, 1056, 1598
SacII CCGCGG 1 cut(s) 1368
SapI GCTCTTC 1 cut(s) 548
SaqAI TTAA 7 cut(s) 327, 596, 1083, 1113, 1623, 1898, 2175
SatI GCNGC 5 cut(s) 90, 1048, 1213, 1293, 2387
Sau3AI GATC 8 cut(s) 78, 119, 886, 1306, 1915, 1999, 2164, 2229
Sau96I GGNCC 3 cut(s) 199, 1011, 1577
ScaI AGTACT 1 cut(s) 315
SchI GAGTC 6 cut(s) 124, 992, 1027, 1449, 1883, 2233
ScrFI CCNGG 3 cut(s) 186, 576, 2391
SduI GDGCHC 2 cut(s) 560, 1595
SfaNI GCATC 4 cut(s) 828, 1708, 2187, 2196
SfcI CTRYAG 3 cut(s) 87, 1045, 2064
Sfr303I CCGCGG 1 cut(s) 1368
SfuI TTCGAA 1 cut(s) 1027
SgrBI CCGCGG 1 cut(s) 1368
SinI GGWCC 2 cut(s) 1011, 1577
SmiMI CAYNNNNRTG 4 cut(s) 47, 839, 1056, 1598
SmlI CTYRAG 3 cut(s) 34, 1130, 1734
SmoI CTYRAG 3 cut(s) 34, 1130, 1734
SnaBI TACGTA 1 cut(s) 802
SpeI ACTAGT 1 cut(s) 316
SphI GCATGC 2 cut(s) 960, 2437
Sse9I AATT 9 cut(s) 358, 693, 731, 1085, 1250, 1350, 1763, 1857, 2073
SsiI CCGC 3 cut(s) 1365, 1367, 1502
SspI AATATT 2 cut(s) 1933, 1989
SspMI CTAG 7 cut(s) 98, 317, 651, 1304, 1893, 1926, 2120
StyD4I CCNGG 3 cut(s) 184, 574, 2389
StyI CCWWGG 2 cut(s) 1051, 1471
TaaI ACNGT 6 cut(s) 8, 226, 241, 373, 594, 831
TaiI ACGT 3 cut(s) 804, 1269, 2289
TaqI TCGA 7 cut(s) 118, 194, 356, 1027, 1992, 2081, 2232
TasI AATT 9 cut(s) 358, 693, 731, 1085, 1250, 1350, 1763, 1857, 2073
TatI WGTACW 5 cut(s) 3, 313, 765, 1407, 1584
TfiI GAWTC 7 cut(s) 278, 379, 1640, 1705, 1750, 2249, 2420
Tru1I TTAA 7 cut(s) 327, 596, 1083, 1113, 1623, 1898, 2175
Tru9I TTAA 7 cut(s) 327, 596, 1083, 1113, 1623, 1898, 2175
TscAI CASTG 3 cut(s) 2365, 2407, 2422
TseFI GTSAC 3 cut(s) 479, 1262, 2413
TseI GCWGC 5 cut(s) 89, 1047, 1212, 1292, 2386
Tsp45I GTSAC 3 cut(s) 479, 1262, 2413
TspGWI ACGGA 5 cut(s) 441, 1026, 1300, 1429, 2430
TspRI CASTG 3 cut(s) 2365, 2407, 2422
Van91I CCANNNNNTGG 1 cut(s) 722
VpaK11BI GGWCC 2 cut(s) 1011, 1577
XagI CCTNNNNNAGG 2 cut(s) 73, 573
XapI RAATTY 5 cut(s) 358, 731, 1250, 1763, 2073
XbaI TCTAGA 1 cut(s) 1892
XceI RCATGY 4 cut(s) 960, 2192, 2335, 2437
XmiI GTMKAC 1 cut(s) 756
XmnI GAANNNNTTC 1 cut(s) 1749
XspI CTAG 7 cut(s) 98, 317, 651, 1304, 1893, 1926, 2120
ZrmI AGTACT 1 cut(s) 315
Zsp2I ATGCAT 1 cut(s) 2194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.