Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0000207

Physical Location & Seq

Genomic Coordinates Reverse (-)

33882812 .. 33886711

Gene Structure 3900 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1232500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2472 bp

ATGGGCGGTTTTGGTTTTCTGCTCATCTGTACTAATTTGCTCCTTCTGCTGCCCACAATTTCCTTTGCAGTTGACAGCATTGGTCCATCGCAGTCTATTCGTGATGGCACGACCTTGGTTGCAAAAGGTGGAAGCTTTGAGCTTGGTTTCTTCAGTCCGGGTAGCTCCAAGAATCGTTACTTGGGAATTTGGTATAAGAACATCCCGGTCCAAACTGTTGTTTGGGTGGCAAACAGATGCAACCCCATTAATGATTCTTCCGGCATGTTGATGATAAACGGAAAAGGGGATCTTGTGCTGCTGGGTCAGAATAAGAGTGTAGTTTGGTCAACGAGCTTAGTTGAACAGCCCAAAAGTGCAACGGTAGAGCTCTTAGATTCCGGGAATTTGGTACTAAGAGATGCAGGGGCAGGAATCTATTTATGGCAAAGCTTTGACTATCCATCTGATACGTTGTTACCAGGAATGAAGTTGGGATGGGACTCGAGGACGGATCTAAATCGTAATTTATCAGCATGGAAAAACTCAGAAGACCCATGTCCGGGAGATTTCATTTGGAAAAACTCAGGACAGCTCGAAATGGAACGTCATATATACCCCGAGGCATATTTTCTTAAAGGCACTGTAAAATACTATCGTTCTGGCCCATGGAATGGCCTAAGATTTAGTGGTGCACCAGAATTAATGCCTAATCCACTTTATAGTTTCAACTTTGTGTATAATGACGATGAAGTGTATTACATGTACACCCTTCTAGATAAGTCTGTAATCTCAAGATTGGTTATGAACCAAACCACGAGTACGCGTGATCGCTTGAGATGGATTGAAGCGGAAAAAACTTGGAGGGCATATTCAACGGTACCCAGGGACATGTGTGATCATTATGGCCTCTGTGGAGCCAACGGAAATTGTCTCATTGGTGAGAATCCAGTCTGCCAATGTCTAAAAGGATTCAAGCCTAAGTCTCAAGAAAGATGGAACTTGACGGACTGGTCTCTAGGATGTGAGCACAAGAAGCCCCTGAGTTGCCAGGAAAAGCATAAGGATGGGTTTATCAAATTTTCCGGCTTGAAATTGCCAGAAACTACACATTCGTGGGTGAACAAAAGTATAAATCTGAAGGAATGCAGAGCGAAATGCTTGAACAACTGTTCCTGTATGGCTTATACAAGCTCGGATATAAGAGGAGGTACTGGCTGCGCCATCTGGTATGGTGATCTAATAGATATCAGGCAGTTTCCAGCTTCTGGACAGGATCTATATGTTCGAATGCCAGCTTCAGAGTTAGAAAATGATGATAAAGCGAAGAAAGCAATTATAGTTGGAGTTCTCGCAGGAGTAGTGTTTGCTGGAATGCTGTTACTTGGATATTATTATTACCTTCACCGAAGAAAGACAGAATTAAAAGGGAAAACTGAAACAAGAGAAAGGGATCGGAACAATGAAGGGGAACCAGAGGGGGAACTGGAGCTTCCGGTCTTTGACCTGACCACAATAGCGAGTGCCACCAATAACTTTTCAAGTGATAACAAGCTCGGAGAAGGTGGCTTTGGACCTGTTTACAAGGGGACGCTAGCAGATGGACAGGAAATTGCTGTGAAGAGGCTTTCGACAAGTTCTGGCCAAGGATTGAGTGAGTTCATGAATGAAGTCGTACTGATTGCCAAGCTCCAGCACCGAAATCTTGTAAAGCTTCTTGGATGCTGTGTTCAAGGAGAAGAGAAAATGCTGATTTATGAATACATGCCCAACAGAGGCCTTGATTCCTTTATTTTTGATAAAACCAGAGAAGAACAAGTCCTAGATTGGCCTACACGCTTCAACATTATATGCGGAATTGCTCGCGGTCTGCTCTACCTCCATCAAGATTCCAGGCTAAGGATTATTCATAGAGATCTCAAAGCAAGTAACGTTTTACTTGATAGTGAAATGAATCCAAAAATTTCAGACTTTGGCTTGGCTAAAACCTTGGTTGGAGGAGATCAGGCTGGTGGAAATACAAAGAAAGTTGTTGGAACATACGGTTACATGGCACCTGAATATGCGGTCGATGGACTATTTTCTGTGAAATCGGATGTCTTTAGCTTTGGTATATTGATGCTAGAGATCATTAGCGGAAGGAAAAACAAGGGATTCTACAATAAAAACAGCCAGAACCTTATTGGGCATGCATGGCAATTATGGAACGAAGGCAGGCCTCTGGAAGTGATTGATACAGGTTTAGGAAATTCGTGTTCTCTATCGGAAGTGTTACGTTGCATCCATGTTAGTCTCTTATGTGTTCAACATGAACCTGAGGATAGGCCAAGCATGGCATCTGTAGTTATAATGTTGGGAAGTGCTGGTGCATTGGCTCAGCCTAAACAACCTGGTTTCTTTATCGAAAAGAAAGCATATGAAGCAGGTCTGTCTTCAGATAATCAAACATCTTCTACCAACGAATTATCAATCACGCTTTTGGAGGCTCGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009506 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019899 GO:0023052 GO:0030054 GO:0031625 GO:0036211 GO:0038023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

824

Amino Acids

91.7

Weight (kDa)

6.66

Isoelectric Point (pI)

39.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	72 - 174	1.2e-36	D-mannose binding lectin
S_locus_glycop	PF00954	212 - 322	3.6e-30	S-locus glycoprotein domain
PAN_2	PF08276	343 - 410	1.5e-22	PAN-like domain
Pkinase	PF00069	507 - 774	1.9e-43	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	509 - 775	2.9e-49	Protein tyrosine and serine/threonine kinase
DUF3403	PF11883	782 - 823	1.3e-07	Domain of unknown function (DUF3403)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000207)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27300
fragaria_vesca	FvH4_3g15930 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g21350 FvH4_3g21400 FvH4_3g21400 FvH4_6g12332
malus_domestica	MD02G1167100.v1.1 MD02G1167200.v1.1 MD03G1185600.v1.1 MD05G1213900.v1.1 MD05G1214100.v1.1 MD05G1214200.v1.1 MD05G1214700.v1.1 MD05G1216300.v1.1 MD05G1216800.v1.1 MD05G1217300.v1.1 MD05G1218000.v1.1 MD11G1231100.v1.1 MD11G1231200.v1.1 MD11G1231400.v1.1 MD11G1231500.v1.1 MD11G1232400.v1.1 MD11G1232500.v1.1
prunus_persica	Prupe.4G142300_v2.0.a1 Prupe.4G142400_v2.0.a1 Prupe.4G142500_v2.0.a1 Prupe.4G142600_v2.0.a1 Prupe.4G142800_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195100_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195500_v2.0.a1 Prupe.4G195700_v2.0.a1 Prupe.4G195900_v2.0.a1
pyrus_communis	pycom03g16540 pycom05g19920 pycom11g20460 pycom11g20480 pycom11g20490 pycom11g20500 pycom11g20530
rosa_chinensis	RchiOBHm_Chr2g0139311 RchiOBHm_Chr2g0139351 RchiOBHm_Chr5g0026801 RchiOBHm_Chr5g0026851 RchiOBHm_Chr5g0026891 RchiOBHm_Chr5g0026911 RchiOBHm_Chr5g0036571 RchiOBHm_Chr5g0036591 RchiOBHm_Chr5g0036721 RchiOBHm_Chr5g0036741 RchiOBHm_Chr7g0222981
rosa_laevigata	RLG00000002006 RLG00000019807 RLG00000032964 RLG00000032968 RLG00000032969 RLG00000033706 RLG00000033708 RLG00000033712 RLG00000033716
rosa_multiflora	Rmu_co8256651.1_g000001 Rmu_co8279377.1_g000001 Rmu_co8429919.1_g000001 Rmu_co8490103.1_g000001 Rmu_sc0000774.1_g000002 Rmu_sc0002525.1_g000012 Rmu_sc0004223.1_g000008 Rmu_sc0004390.1_g000002 Rmu_sc0006059.1_g000014 Rmu_sc0006059.1_g000024 Rmu_sc0006514.1_g000020 Rmu_sc0014333.1_g000002 Rmu_ssc0000172.1_g000018 Rmu_ssc0000172.1_g000029
rosa_roxburghii	Rroxscaffold_1G00044230 Rroxscaffold_1G00044260 Rroxscaffold_1G00044320 Rroxscaffold_1G00044340 Rroxscaffold_1G00052560 Rroxscaffold_1G00052570 Rroxscaffold_1G00052660 Rroxscaffold_1G00052720 Rroxscaffold_2G00105450 Rroxscaffold_2G00105460
rosa_rugosa	Rorug02G0356900 Rorug02G0357000 Rorug02G0357100 Rorug02G0357200 Rorug02G0357300 Rorug02G0357400 Rorug02G0357500 Rorug02G0357600 Rorug05G0095400 Rorug05G0095500 Rorug05G0095500 Rorug05G0095600 Rorug05G0095700 Rorug05G0095800 Rorug05G0157700 Rorug05G0157800
rosa_samantha	Rh2AG406800 Rh2AG406900 Rh2BG417300 Rh2CG392900 Rh2DG426200 Rh5AG189300 Rh5AG249400 Rh5AG250100 Rh5BG186300 Rh5BG186900 Rh5BG187000 Rh5BG249500 Rh5BG250600 Rh5BG250900 Rh5BG251500 Rh5BG251800 Rh5CG205500 Rh5CG206300 Rh5CG206700 Rh5CG283000 Rh5CG283300 Rh5CG283500 Rh5DG188500 Rh5DG188700 Rh5DG258700 Rh5DG258900 Rh5DG259600 Rh5DG259900 Rh7CG375800 Rh7DG352500
rosa_wichuraiana	Rw2G032960 Rw2G032970 Rw2G033220 Rw2G033230 Rw5G017130 Rw5G017230 Rw5G022940 Rw5G022960 Rw5G022980 Rw7G030420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2327
AasI	GACNNNNNNGTC	1 cut(s)	991
Acc36I	ACCTGC	1 cut(s)	2393
Acc65I	GGTACC	1 cut(s)	859
AccB1I	GGYRCC	2 cut(s)	859, 2032
AccB7I	CCANNNNNTGG	2 cut(s)	653, 1247
AccII	CGCG	2 cut(s)	805, 1845
AciI	CCGC	6 cut(s)	6, 830, 1833, 1845, 2045, 2115
AclI	AACGTT	1 cut(s)	1911
AclWI	GGATC	4 cut(s)	297, 501, 1263, 1440
AcoI	YGGCCR	1 cut(s)	1621
AcsI	RAATTY	5 cut(s)	186, 385, 1058, 1941, 2227
AcuI	CTGAAG	4 cut(s)	136, 1139, 1263, 2397
AfaI	GTAC	7 cut(s)	31, 393, 746, 802, 861, 1192, 1656
AfiI	CCNNNNNNNGG	6 cut(s)	541, 542, 653, 1247, 1754, 1878
AflIII	ACRYGT	3 cut(s)	741, 803, 870
AjnI	CCWGG	5 cut(s)	460, 863, 1029, 1871, 2368
AjuI	GAANNNNNNNTTGG	4 cut(s)	164, 196, 1533, 1565
AleI	CACNNNNGTG	1 cut(s)	1093
AloI	GAACNNNNNNTCC	4 cut(s)	1136, 1168, 1692, 1724
Alw21I	GWGCWC	3 cut(s)	372, 676, 1011
Alw26I	GTCTC	4 cut(s)	917, 969, 999, 2276
Alw44I	GTGCAC	1 cut(s)	672
AlwI	GGATC	4 cut(s)	297, 501, 1263, 1440
AlwNI	CAGNNNCTG	1 cut(s)	1247
Ama87I	CYCGRG	2 cut(s)	484, 599
AoxI	GGCC	8 cut(s)	643, 655, 886, 1621, 1756, 1808, 2195, 2303
ApaLI	GTGCAC	1 cut(s)	672
ApeKI	GCWGC	3 cut(s)	49, 298, 1197
ApoI	RAATTY	5 cut(s)	186, 385, 1058, 1941, 2227
AseI	ATTAAT	2 cut(s)	249, 683
Asp718I	GGTACC	1 cut(s)	859
AspLEI	GCGC	1 cut(s)	1202
AspS9I	GGNCC	4 cut(s)	83, 208, 644, 1553
AsuC2I	CCSGG	4 cut(s)	159, 206, 382, 543
AsuHPI	GGTGA	4 cut(s)	932, 1111, 1226, 1376
AsuII	TTCGAA	1 cut(s)	1267
AsuNHI	GCTAGC	1 cut(s)	1573
AvaI	CYCGRG	2 cut(s)	484, 599
AvaII	GGWCC	3 cut(s)	83, 208, 1553
AxyI	CCTNAGG	1 cut(s)	2295
BaeGI	GKGCMC	1 cut(s)	676
BalI	TGGCCA	1 cut(s)	1623
BanI	GGYRCC	2 cut(s)	859, 2032
BanII	GRGCYC	1 cut(s)	372
BauI	CACGAG	1 cut(s)	796
BbsI	GAAGAC	2 cut(s)	537, 2403
Bbv12I	GWGCWC	3 cut(s)	372, 676, 1011
BbvI	GCAGC	3 cut(s)	36, 285, 1184
BcgI	CGANNNNNNTGC	2 cut(s)	80, 114
BciT130I	CCWGG	5 cut(s)	462, 865, 1031, 1873, 2370
BclI	TGATCA	1 cut(s)	877
BcnI	CCSGG	4 cut(s)	159, 206, 382, 543
BcoDI	GTCTC	4 cut(s)	917, 969, 999, 2276
BfaI	CTAG	5 cut(s)	755, 998, 1574, 1802, 2102
BfmI	CTRYAG	1 cut(s)	2319
BfuAI	ACCTGC	1 cut(s)	2393
BglII	AGATCT	1 cut(s)	1894
BisI	GCNGC	3 cut(s)	50, 299, 1198
BlpI	GCTNAGC	1 cut(s)	2355
BlsI	GCNGC	3 cut(s)	51, 300, 1199
Bme1390I	CCNGG	9 cut(s)	159, 206, 382, 462, 543, 865, 1031, 1873, 2370
Bme18I	GGWCC	3 cut(s)	83, 208, 1553
BmeT110I	CYCGRG	2 cut(s)	484, 599
BmgT120I	GGNCC	4 cut(s)	83, 208, 644, 1553
BmiI	GGNNCC	4 cut(s)	861, 898, 1452, 2034
BmrFI	CCNGG	9 cut(s)	159, 206, 382, 462, 543, 865, 1031, 1873, 2370
BmsI	GCATC	6 cut(s)	227, 391, 1691, 2088, 2268, 2324
BmtI	GCTAGC	1 cut(s)	1577
BoxI	GACNNNNGTC	1 cut(s)	537
BpiI	GAAGAC	2 cut(s)	537, 2403
BpmI	CTGGAG	2 cut(s)	1487, 1655
Bpu10I	CCTNAGC	1 cut(s)	1877
Bpu1102I	GCTNAGC	1 cut(s)	2355
Bpu14I	TTCGAA	1 cut(s)	1267
BpuEI	CTTGAG	3 cut(s)	757, 835, 951
BpuMI	CCSGG	4 cut(s)	159, 206, 382, 543
BsaBI	GATNNNNATC	1 cut(s)	498
BsaI	GGTCTC	1 cut(s)	999
BsaJI	CCNNGG	7 cut(s)	114, 600, 647, 863, 864, 1624, 1968
BsaWI	WCCGGW	1 cut(s)	1474
Bsc4I	CCNNNNNNNGG	6 cut(s)	541, 542, 653, 1247, 1754, 1878
Bse1I	ACTGG	4 cut(s)	929, 995, 1198, 1470
Bse21I	CCTNAGG	1 cut(s)	2295
Bse8I	GATNNNNATC	1 cut(s)	498
BseBI	CCWGG	5 cut(s)	462, 865, 1031, 1873, 2370
BseDI	CCNNGG	7 cut(s)	114, 600, 647, 863, 864, 1624, 1968
BseGI	GGATG	7 cut(s)	201, 482, 1007, 1051, 1706, 2080, 2259
BseJI	GATNNNNATC	1 cut(s)	498
BseLI	CCNNNNNNNGG	6 cut(s)	541, 542, 653, 1247, 1754, 1878
BseMII	CTCAG	5 cut(s)	540, 579, 1013, 2286, 2369
BseNI	ACTGG	4 cut(s)	929, 995, 1198, 1470
BseRI	GAGGAG	2 cut(s)	1200, 1992
BseSI	GKGCMC	1 cut(s)	676
BseXI	GCAGC	3 cut(s)	36, 285, 1184
BseYI	CCCAGC	1 cut(s)	301
Bsh1236I	CGCG	2 cut(s)	805, 1845
Bsh1285I	CGRYCG	1 cut(s)	2049
BshFI	GGCC	8 cut(s)	645, 657, 888, 1623, 1758, 1810, 2197, 2305
BshNI	GGYRCC	2 cut(s)	859, 2032
BsiEI	CGRYCG	1 cut(s)	2049
BsiHKAI	GWGCWC	3 cut(s)	372, 676, 1011
BsiHKCI	CYCGRG	2 cut(s)	484, 599
BsiSI	CCGG	7 cut(s)	158, 206, 261, 381, 542, 1065, 1475
BslFI	GGGAC	3 cut(s)	494, 881, 1582
BslI	CCNNNNNNNGG	6 cut(s)	541, 542, 653, 1247, 1754, 1878
BsmAI	GTCTC	4 cut(s)	917, 969, 999, 2276
BsmFI	GGGAC	3 cut(s)	494, 881, 1582
BsmI	GAATGC	3 cut(s)	1130, 1275, 1359
BsnI	GGCC	8 cut(s)	645, 657, 888, 1623, 1758, 1810, 2197, 2305
Bso31I	GGTCTC	1 cut(s)	999
BsoBI	CYCGRG	2 cut(s)	484, 599
Bsp119I	TTCGAA	1 cut(s)	1267
Bsp1286I	GDGCHC	3 cut(s)	372, 676, 1011
Bsp1407I	TGTACA	1 cut(s)	744
Bsp1720I	GCTNAGC	1 cut(s)	2355
Bsp19I	CCATGG	1 cut(s)	647
BspACI	CCGC	6 cut(s)	6, 830, 1833, 1845, 2045, 2115
BspANI	GGCC	8 cut(s)	645, 657, 888, 1623, 1758, 1810, 2197, 2305
BspCNI	CTCAG	5 cut(s)	539, 578, 1014, 2287, 2368
BspFNI	CGCG	2 cut(s)	805, 1845
BspHI	TCATGA	1 cut(s)	1641
BspLI	GGNNCC	4 cut(s)	861, 898, 1452, 2034
BspMI	ACCTGC	1 cut(s)	2393
BspOI	GCTAGC	1 cut(s)	1577
BspPI	GGATC	4 cut(s)	297, 501, 1263, 1440
BspT104I	TTCGAA	1 cut(s)	1267
BspT107I	GGYRCC	2 cut(s)	859, 2032
BspTNI	GGTCTC	1 cut(s)	999
BsrGI	TGTACA	1 cut(s)	744
BsrI	ACTGG	4 cut(s)	929, 995, 1198, 1470
BssECI	CCNNGG	7 cut(s)	114, 600, 647, 863, 864, 1624, 1968
BssSI	CACGAG	1 cut(s)	796
BssT1I	CCWWGG	4 cut(s)	114, 647, 1624, 1968
Bst2BI	CACGAG	1 cut(s)	796
Bst2UI	CCWGG	5 cut(s)	462, 865, 1031, 1873, 2370
Bst4CI	ACNGT	6 cut(s)	217, 364, 625, 859, 1151, 2024
Bst6I	CTCTTC	2 cut(s)	1595, 1713
BstAUI	TGTACA	1 cut(s)	744
BstBI	TTCGAA	1 cut(s)	1267
BstC8I	GCNNGC	5 cut(s)	1275, 1575, 1843, 2169, 2195
BstDSI	CCRYGG	1 cut(s)	647
BstF5I	GGATG	7 cut(s)	201, 482, 1007, 1051, 1706, 2080, 2259
BstFNI	CGCG	2 cut(s)	805, 1845
BstHHI	GCGC	1 cut(s)	1202
BstMAI	GTCTC	4 cut(s)	917, 969, 999, 2276
BstMCI	CGRYCG	1 cut(s)	2049
BstMWI	GCNNNNNNNGC	5 cut(s)	46, 1015, 1310, 2173, 2399
BstNI	CCWGG	5 cut(s)	462, 865, 1031, 1873, 2370
BstNSI	RCATGY	5 cut(s)	268, 745, 874, 1747, 2171
BstPAI	GACNNNNGTC	1 cut(s)	537
BstSCI	CCNGG	9 cut(s)	157, 204, 380, 460, 541, 863, 1029, 1871, 2368
BstSFI	CTRYAG	1 cut(s)	2319
BstSLI	GKGCMC	1 cut(s)	676
BstUI	CGCG	2 cut(s)	805, 1845
BstV1I	GCAGC	3 cut(s)	36, 285, 1184
BstV2I	GAAGAC	2 cut(s)	537, 2403
BstX2I	RGATCY	4 cut(s)	289, 493, 1255, 1894
BstYI	RGATCY	4 cut(s)	289, 493, 1255, 1894
Bsu36I	CCTNAGG	1 cut(s)	2295
BsuRI	GGCC	8 cut(s)	645, 657, 888, 1623, 1758, 1810, 2197, 2305
BtgI	CCRYGG	1 cut(s)	647
BtgZI	GCGATG	1 cut(s)	72
BtsCI	GGATG	7 cut(s)	201, 482, 1007, 1051, 1706, 2080, 2259
BtsIMutI	CAGTG	1 cut(s)	621
BveI	ACCTGC	1 cut(s)	2393
Cac8I	GCNNGC	5 cut(s)	1275, 1575, 1843, 2169, 2195
CaiI	CAGNNNCTG	1 cut(s)	1247
CciI	TCATGA	1 cut(s)	1641
CfoI	GCGC	1 cut(s)	1202
Cfr13I	GGNCC	4 cut(s)	83, 208, 644, 1553
CseI	GACGC	1 cut(s)	1579
CsiI	ACCWGGT	1 cut(s)	2368
Csp6I	GTAC	7 cut(s)	30, 392, 745, 801, 860, 1191, 1655
CviQI	GTAC	7 cut(s)	30, 392, 745, 801, 860, 1191, 1655
DrdI	GACNNNNNNGTC	1 cut(s)	991
DseDI	GACNNNNNNGTC	1 cut(s)	991
EaeI	YGGCCR	1 cut(s)	1621
Eam1104I	CTCTTC	2 cut(s)	1595, 1713
EarI	CTCTTC	2 cut(s)	1595, 1713
Ecl136II	GAGCTC	1 cut(s)	370
Eco130I	CCWWGG	4 cut(s)	114, 647, 1624, 1968
Eco147I	AGGCCT	2 cut(s)	1758, 2197
Eco24I	GRGCYC	1 cut(s)	372
Eco31I	GGTCTC	1 cut(s)	999
Eco32I	GATATC	1 cut(s)	1228
Eco47I	GGWCC	3 cut(s)	83, 208, 1553
Eco53kI	GAGCTC	1 cut(s)	370
Eco57I	CTGAAG	4 cut(s)	136, 1139, 1263, 2397
Eco81I	CCTNAGG	1 cut(s)	2295
Eco88I	CYCGRG	2 cut(s)	484, 599
EcoICRI	GAGCTC	1 cut(s)	370
EcoRII	CCWGG	5 cut(s)	460, 863, 1029, 1871, 2368
EcoRV	GATATC	1 cut(s)	1228
EcoT14I	CCWWGG	4 cut(s)	114, 647, 1624, 1968
EcoT22I	ATGCAT	1 cut(s)	2173
EcoT38I	GRGCYC	1 cut(s)	372
ErhI	CCWWGG	4 cut(s)	114, 647, 1624, 1968
FalI	AAGNNNNNCTT	2 cut(s)	276, 308
FaqI	GGGAC	3 cut(s)	494, 881, 1582
FauNDI	CATATG	1 cut(s)	2395
FbaI	TGATCA	1 cut(s)	877
Fnu4HI	GCNGC	3 cut(s)	50, 299, 1198
FokI	GGATG	7 cut(s)	188, 489, 1014, 1058, 1713, 2087, 2246
FriOI	GRGCYC	1 cut(s)	372
Fsp4HI	GCNGC	3 cut(s)	50, 299, 1198
FspBI	CTAG	5 cut(s)	755, 998, 1574, 1802, 2102
GlaI	GCGC	1 cut(s)	1201
GluI	GCNGC	3 cut(s)	50, 299, 1198
GsaI	CCCAGC	1 cut(s)	305
GsuI	CTGGAG	2 cut(s)	1487, 1655
HaeIII	GGCC	8 cut(s)	645, 657, 888, 1623, 1758, 1810, 2197, 2305
HapII	CCGG	7 cut(s)	158, 206, 261, 381, 542, 1065, 1475
HgaI	GACGC	1 cut(s)	1579
HhaI	GCGC	1 cut(s)	1202
Hin6I	GCGC	1 cut(s)	1200
HinP1I	GCGC	1 cut(s)	1200
HincII	GTYRAC	2 cut(s)	73, 330
HindII	GTYRAC	2 cut(s)	73, 330
HindIII	AAGCTT	3 cut(s)	133, 430, 1691
HpaII	CCGG	7 cut(s)	158, 206, 261, 381, 542, 1065, 1475
HphI	GGTGA	4 cut(s)	932, 1111, 1226, 1376
Hpy166II	GTNNAC	6 cut(s)	73, 330, 674, 747, 1102, 1561
Hpy188III	TCNNGA	9 cut(s)	101, 567, 755, 774, 968, 1248, 1642, 1865, 2201
Hpy8I	GTNNAC	6 cut(s)	73, 330, 674, 747, 1102, 1561
HpyAV	CCTTC	8 cut(s)	53, 761, 1114, 1391, 1439, 1535, 2112, 2183
HpyCH4III	ACNGT	6 cut(s)	217, 364, 625, 859, 1151, 2024
HpyCH4IV	ACGT	4 cut(s)	452, 586, 1911, 2254
HpyF10VI	GCNNNNNNNGC	5 cut(s)	46, 1015, 1310, 2173, 2399
HpySE526I	ACGT	4 cut(s)	452, 586, 1911, 2254
HspAI	GCGC	1 cut(s)	1200
KpnI	GGTACC	1 cut(s)	863
Ksp22I	TGATCA	1 cut(s)	877
LmnI	GCTCC	5 cut(s)	45, 170, 896, 1468, 1674
Lsp1109I	GCAGC	3 cut(s)	36, 285, 1184
LweI	GCATC	6 cut(s)	227, 391, 1691, 2088, 2268, 2324
MabI	ACCWGGT	1 cut(s)	2368
MaeI	CTAG	5 cut(s)	755, 998, 1574, 1802, 2102
MaeII	ACGT	4 cut(s)	452, 586, 1911, 2254
MaeIII	GTNAC	6 cut(s)	176, 456, 1359, 1907, 2024, 2250
MflI	RGATCY	4 cut(s)	289, 493, 1255, 1894
MhlI	GDGCHC	3 cut(s)	372, 676, 1011
MlsI	TGGCCA	1 cut(s)	1623
MluI	ACGCGT	1 cut(s)	803
MluNI	TGGCCA	1 cut(s)	1623
MlyI	GAGTC	1 cut(s)	476
MmeI	TCCRAC	3 cut(s)	1303, 1954, 1993
Mox20I	TGGCCA	1 cut(s)	1623
Mph1103I	ATGCAT	1 cut(s)	2173
MscI	TGGCCA	1 cut(s)	1623
MseI	TTAA	5 cut(s)	249, 615, 683, 1403, 2470
MslI	CAYNNNNRTG	3 cut(s)	269, 1044, 1093
Msp20I	TGGCCA	1 cut(s)	1623
MspI	CCGG	7 cut(s)	158, 206, 261, 381, 542, 1065, 1475
MspR9I	CCNGG	9 cut(s)	159, 206, 382, 462, 543, 865, 1031, 1873, 2370
Mva1269I	GAATGC	3 cut(s)	1130, 1275, 1359
MvaI	CCWGG	5 cut(s)	462, 865, 1031, 1873, 2370
MvnI	CGCG	2 cut(s)	805, 1845
MwoI	GCNNNNNNNGC	5 cut(s)	46, 1015, 1310, 2173, 2399
NciI	CCSGG	4 cut(s)	159, 206, 382, 543
NcoI	CCATGG	1 cut(s)	647
NdeI	CATATG	1 cut(s)	2395
NheI	GCTAGC	1 cut(s)	1573
NlaIV	GGNNCC	4 cut(s)	861, 898, 1452, 2034
NsiI	ATGCAT	1 cut(s)	2173
NspI	RCATGY	5 cut(s)	268, 745, 874, 1747, 2171
NspV	TTCGAA	1 cut(s)	1267
OliI	CACNNNNGTG	1 cut(s)	1093
PaeI	GCATGC	1 cut(s)	2171
PaeR7I	CTCGAG	1 cut(s)	484
PagI	TCATGA	1 cut(s)	1641
PasI	CCCWGGG	1 cut(s)	864
PceI	AGGCCT	2 cut(s)	1758, 2197
PciI	ACATGT	2 cut(s)	741, 870
PctI	GAATGC	3 cut(s)	1130, 1275, 1359
PflMI	CCANNNNNTGG	2 cut(s)	653, 1247
PfoI	TCCNGGA	2 cut(s)	380, 541
PkrI	GCNGC	3 cut(s)	51, 300, 1199
PleI	GAGTC	1 cut(s)	476
PpsI	GAGTC	1 cut(s)	476
PscI	ACATGT	2 cut(s)	741, 870
PshAI	GACNNNNGTC	1 cut(s)	537
PshBI	ATTAAT	2 cut(s)	249, 683
PsiI	TTATAA	1 cut(s)	2327
Psp124BI	GAGCTC	1 cut(s)	372
Psp1406I	AACGTT	1 cut(s)	1911
Psp6I	CCWGG	5 cut(s)	460, 863, 1029, 1871, 2368
PspFI	CCCAGC	1 cut(s)	301
PspGI	CCWGG	5 cut(s)	460, 863, 1029, 1871, 2368
PspN4I	GGNNCC	4 cut(s)	861, 898, 1452, 2034
PspPI	GGNCC	4 cut(s)	83, 208, 644, 1553
PspXI	VCTCGAGB	1 cut(s)	484
PstNI	CAGNNNCTG	1 cut(s)	1247
PsuI	RGATCY	4 cut(s)	289, 493, 1255, 1894
RsaI	GTAC	7 cut(s)	31, 393, 746, 802, 861, 1192, 1656
RsaNI	GTAC	7 cut(s)	30, 392, 745, 801, 860, 1191, 1655
RseI	CAYNNNNRTG	3 cut(s)	269, 1044, 1093
SacI	GAGCTC	1 cut(s)	372
SaqAI	TTAA	5 cut(s)	249, 615, 683, 1403, 2470
SatI	GCNGC	3 cut(s)	50, 299, 1198
Sau96I	GGNCC	4 cut(s)	83, 208, 644, 1553
SchI	GAGTC	1 cut(s)	476
ScrFI	CCNGG	9 cut(s)	159, 206, 382, 462, 543, 865, 1031, 1873, 2370
SduI	GDGCHC	3 cut(s)	372, 676, 1011
SexAI	ACCWGGT	1 cut(s)	2368
SfaNI	GCATC	6 cut(s)	227, 391, 1691, 2088, 2268, 2324
SfcI	CTRYAG	1 cut(s)	2319
Sfr274I	CTCGAG	1 cut(s)	484
SfuI	TTCGAA	1 cut(s)	1267
SinI	GGWCC	3 cut(s)	83, 208, 1553
SlaI	CTCGAG	1 cut(s)	484
SmiMI	CAYNNNNRTG	3 cut(s)	269, 1044, 1093
SmlI	CTYRAG	4 cut(s)	484, 772, 814, 966
SmoI	CTYRAG	4 cut(s)	484, 772, 814, 966
SphI	GCATGC	1 cut(s)	2171
SseBI	AGGCCT	2 cut(s)	1758, 2197
SsiI	CCGC	6 cut(s)	6, 830, 1833, 1845, 2045, 2115
SspMI	CTAG	5 cut(s)	755, 998, 1574, 1802, 2102
SstI	GAGCTC	1 cut(s)	372
StuI	AGGCCT	2 cut(s)	1758, 2197
StyD4I	CCNGG	9 cut(s)	157, 204, 380, 460, 541, 863, 1029, 1871, 2368
StyI	CCWWGG	4 cut(s)	114, 647, 1624, 1968
TaaI	ACNGT	6 cut(s)	217, 364, 625, 859, 1151, 2024
TaiI	ACGT	4 cut(s)	455, 589, 1914, 2257
TaqI	TCGA	6 cut(s)	485, 576, 1267, 1610, 2049, 2382
TatI	WGTACW	2 cut(s)	29, 744
Tru1I	TTAA	5 cut(s)	249, 615, 683, 1403, 2470
Tru9I	TTAA	5 cut(s)	249, 615, 683, 1403, 2470
TscAI	CASTG	1 cut(s)	628
TseI	GCWGC	3 cut(s)	49, 298, 1197
TspGWI	ACGGA	4 cut(s)	294, 506, 918, 1001
TspRI	CASTG	1 cut(s)	628
Van91I	CCANNNNNTGG	2 cut(s)	653, 1247
VneI	GTGCAC	1 cut(s)	672
VpaK11BI	GGWCC	3 cut(s)	83, 208, 1553
VspI	ATTAAT	2 cut(s)	249, 683
XapI	RAATTY	5 cut(s)	186, 385, 1058, 1941, 2227
XbaI	TCTAGA	1 cut(s)	754
XceI	RCATGY	5 cut(s)	268, 745, 874, 1747, 2171
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2159
XhoI	CTCGAG	1 cut(s)	484
XspI	CTAG	5 cut(s)	755, 998, 1574, 1802, 2102
Zsp2I	ATGCAT	1 cut(s)	2173

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.