RchiOBHm_Chr5g0026891

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000207
Physical Location & Seq
Reverse (-)
20868549 .. 20873633
5085 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30647

Sequence Viewer

Length: 2394 bp
ATGTTACAAACTCCGATGGTAAAGCATAACGTGAATCAGTTTCATTGGGCGTGTGGAAGCTATGAACTGGGATTCTTTAGTCCCGGTAAAGTGAAAGGCAGATACTTGGGAATATGGTACACCTTTTCTACTGAGACAGTTGTATGGGTAGCGAACAGAGAAATACCACTTAATGATTCTTTAGGAGTTTTAAAGGTCACTGATCAGGGAGTTTTAGTCCTTCTGAATAGCTCAAATGGCACTGTATGGTCATCCAATTCATCAAGAACCGCAGGGAATCCAGTCTCACAACTCTTGGATTCGGGAAATCTTGTTGTGAAAGATGGAAATGAAACTAATCCTGATAACTTCTTGTGGCAGAGTTTTGATTATCCTTGTGATACACGCCTGCCAGAAATGAAGCTTGGTTGGAATTTAGTTACTGGTTTAGATAGGTATGTCTCGTCTTGGAGGAGCACAGAAGATCCTGCTCAAGGAGAGTTTTTGCTACAGATGGATACTCATGGTTTCCCACAGATTTTTATTATGAAGGGAGCTAAGATACTGACTAGAGCAGGGACATGGAACGGCGTTGAATTAACTGGATATCAAGGAAAGCCAAATCCAGTAGCGGTTTTTGAGTTTGTGTTGACTAAGGATGAAGTCTATTATGAGTACACACTCCTCAACCGGTCAATGTTTGCAAGATATGTATTAAACCCATATGGCATTGCACAGTGGCTTACTTGGACAGACCACTCACGTAGTTGGGAACCTTTCATCTCAACTCAGGCAGATCAGTGTGAAAATTATGCCTATTGTGGTGCTAATGCTAGATGTAATGTCAGTAACACTCCTGTATGTACATGCTTGAAAGGGTTTGTACCAAAATCTCTGAAAGATTGGAACTCAACAAATTGGTCTGGGGGATGTGTTCGGGAGACTCCATTAGCTTGCAGCTCTACAGATGGCTTCTCAAAGTACAGTAACTTTAAATTGCCAGACACATCTTCTTCCTGGTATGACAAGAGCATGACCCTCAAGGAGTGCCAAGGATTATGTTTGGGAAACTGTTCATGTACTGCATATGCGAATTTAGATTTCAGGGAAGGTGGAAGTGGCTGCTTGCTTTGGTTTGGGAACCTCAATGACATTAGAGAATTCACCTCTGATTCTCAAGACCTCTTTATACGGCTGGCTGCTTCAGACCTAGATCCTATTGTGAAAAAGAGCAAGTCCAGCAAGAAAAAGCTAGCAGGAATTCTGATCGGCTCATCTGTATTTCTTGTGGGAATGCTAATAGTTGGGTTCATTTTGTATAAACGGAAGAGGAAACTCAGAAAGCAAGGAGCCAGAAGAAAGCTGGATTGCAGAAGGGAGGATTACCGTGGAGAAGACAGGGAAGACTTGGAGTTACCACTGTTTGACTTGACCACTATTGCTAATGCCACTAATGACTTTTCAAGCAGCAACAAGCTGGGTGAAGGTGGTTTTGGACCTGTGTACAAGGGAACATTGATTGGAGGAGAGGAAATTGCAGTAAAGAGGCTCTCAAAAAATTCTGGACAGGGATTGAGAGAGTTTAAAAACGAAGTTCTACTGATAGCCAAACTTCAGCATCGGAATCTTGTAAAGCTTCTTGGTTGTTGCATTCAAGAAGATGAAGAAATTCTAGTATATGAATACATGCCTAACAGAAGCTTGGACTTCTTTATATTTGATGAAGAAAGACAGAAGTTGCTCGACTGGCCCACATGCTTCCATATTATTGAGGGAATTGCTCGAGGGCTTCTTTATCTCCATCAAGACTCTAGATTAAGAATTATCCATAGAGATATGAAGGCTAGCAATATCTTGCTAGACAATGATATGAACCCAAAGATTTCAGACTTCGGCCTGGCTAAAACATTTGGGGGTGATCAAAGTGCAGACAGTACAAATAGAGTGGTGGGAACATATGGTTATATGGCTCCTGAATATGCAGTAGATGGAACTTACTCAATGAAATCCGATGTATTTAGCTTTGGAGTTGTACTGCTAGAGATATTGAGCAGGAAGAAGAACAGGGGATTTTGTCATCCAGATCACCATCTTAACCTTCTTGGACATGCATGGACACTATGGACTCAAAATAATCCTCTGGAACTGGTTGATGTGACGTTATGTGATTCTTGCAACACATCCGGAGTGTTGAGATGTCTTCATGTGGGCCTGTTATGTGTGCAACAAGCACCTGAAGATAGACCAAGCATGTCATCTGTGGTTCTAATGTTGGGAAGTGAAATCAAGTTGCCTACGCCAAAAGAGCCTGGTTTTTACACTGGACGGACCGTTCCAGTTCCTGAATCAACATCAACGACAAGTGACTTGTGCTCAGCAAATAGTTTCAGCACAATGTTCTTAGAATCTCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

797

Amino Acids

89.42

Weight (kDa)

6.4

Isoelectric Point (pI)

38.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 46 - 151 2.4e-37 D-mannose binding lectin
S_locus_glycop PF00954 184 - 291 5.6e-24 S-locus glycoprotein domain
PAN_2 PF08276 312 - 378 1.3e-21 PAN-like domain
Pkinase PF00069 481 - 680 7.8e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 483 - 748 1.8e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27300
fragaria_vesca FvH4_3g15930 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g21350 FvH4_3g21400 FvH4_3g21400 FvH4_6g12332
malus_domestica MD02G1167100.v1.1 MD02G1167200.v1.1 MD03G1185600.v1.1 MD05G1213900.v1.1 MD05G1214100.v1.1 MD05G1214200.v1.1 MD05G1214700.v1.1 MD05G1216300.v1.1 MD05G1216800.v1.1 MD05G1217300.v1.1 MD05G1218000.v1.1 MD11G1231100.v1.1 MD11G1231200.v1.1 MD11G1231400.v1.1 MD11G1231500.v1.1 MD11G1232400.v1.1 MD11G1232500.v1.1
prunus_persica Prupe.4G142300_v2.0.a1 Prupe.4G142400_v2.0.a1 Prupe.4G142500_v2.0.a1 Prupe.4G142600_v2.0.a1 Prupe.4G142800_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195100_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195500_v2.0.a1 Prupe.4G195700_v2.0.a1 Prupe.4G195900_v2.0.a1
pyrus_communis pycom03g16540 pycom05g19920 pycom11g20460 pycom11g20480 pycom11g20490 pycom11g20500 pycom11g20530
rosa_chinensis RchiOBHm_Chr2g0139311 RchiOBHm_Chr2g0139351 RchiOBHm_Chr5g0026801 RchiOBHm_Chr5g0026851 RchiOBHm_Chr5g0026891 RchiOBHm_Chr5g0026911 RchiOBHm_Chr5g0036571 RchiOBHm_Chr5g0036591 RchiOBHm_Chr5g0036721 RchiOBHm_Chr5g0036741 RchiOBHm_Chr7g0222981
rosa_laevigata RLG00000002006 RLG00000019807 RLG00000032964 RLG00000032968 RLG00000032969 RLG00000033706 RLG00000033708 RLG00000033712 RLG00000033716
rosa_multiflora Rmu_co8256651.1_g000001 Rmu_co8279377.1_g000001 Rmu_co8429919.1_g000001 Rmu_co8490103.1_g000001 Rmu_sc0000774.1_g000002 Rmu_sc0002525.1_g000012 Rmu_sc0004223.1_g000008 Rmu_sc0004390.1_g000002 Rmu_sc0006059.1_g000014 Rmu_sc0006059.1_g000024 Rmu_sc0006514.1_g000020 Rmu_sc0014333.1_g000002 Rmu_ssc0000172.1_g000018 Rmu_ssc0000172.1_g000029
rosa_roxburghii Rroxscaffold_1G00044230 Rroxscaffold_1G00044260 Rroxscaffold_1G00044320 Rroxscaffold_1G00044340 Rroxscaffold_1G00052560 Rroxscaffold_1G00052570 Rroxscaffold_1G00052660 Rroxscaffold_1G00052720 Rroxscaffold_2G00105450 Rroxscaffold_2G00105460
rosa_rugosa Rorug02G0356900 Rorug02G0357000 Rorug02G0357100 Rorug02G0357200 Rorug02G0357300 Rorug02G0357400 Rorug02G0357500 Rorug02G0357600 Rorug05G0095400 Rorug05G0095500 Rorug05G0095500 Rorug05G0095600 Rorug05G0095700 Rorug05G0095800 Rorug05G0157700 Rorug05G0157800
rosa_samantha Rh2AG406800 Rh2AG406900 Rh2BG417300 Rh2CG392900 Rh2DG426200 Rh5AG189300 Rh5AG249400 Rh5AG250100 Rh5BG186300 Rh5BG186900 Rh5BG187000 Rh5BG249500 Rh5BG250600 Rh5BG250900 Rh5BG251500 Rh5BG251800 Rh5CG205500 Rh5CG206300 Rh5CG206700 Rh5CG283000 Rh5CG283300 Rh5CG283500 Rh5DG188500 Rh5DG188700 Rh5DG258700 Rh5DG258900 Rh5DG259600 Rh5DG259900 Rh7CG375800 Rh7DG352500
rosa_wichuraiana Rw2G032960 Rw2G032970 Rw2G033220 Rw2G033230 Rw5G017130 Rw5G017230 Rw5G022940 Rw5G022960 Rw5G022980 Rw7G030420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 2162
AciI CCGC 2 cut(s) 270, 611
AclWI GGATC 2 cut(s) 458, 1187
AcsI RAATTY 6 cut(s) 412, 1072, 1139, 1239, 1537, 1647
AcuI CTGAAG 3 cut(s) 1167, 1577, 2235
AfaI GTAC 9 cut(s) 119, 656, 844, 864, 962, 1060, 1484, 1915, 2013
AfiI CCNNNNNNNGG 1 cut(s) 473
AgeI ACCGGT 1 cut(s) 669
AgsI TTSAA 4 cut(s) 575, 853, 1443, 1634
AjnI CCWGG 3 cut(s) 995, 1875, 2287
AjuI GAANNNNNNNTTGG 4 cut(s) 387, 419, 1455, 1487
AloI GAACNNNNNNTCC 2 cut(s) 2295, 2327
Alw21I GWGCWC 2 cut(s) 458, 2354
Alw26I GTCTC 4 cut(s) 128, 289, 445, 914
AlwI GGATC 2 cut(s) 458, 1187
AlwNI CAGNNNCTG 1 cut(s) 2321
Ama87I CYCGRG 1 cut(s) 1761
Aor13HI TCCGGA 1 cut(s) 2162
AoxI GGCC 3 cut(s) 1727, 1873, 2188
ApeKI GCWGC 4 cut(s) 936, 1101, 1178, 1446
ApoI RAATTY 6 cut(s) 412, 1072, 1139, 1239, 1537, 1647
AsiGI ACCGGT 1 cut(s) 669
Asp700I GAANNNNTTC 2 cut(s) 1051, 1647
AspS9I GGNCC 4 cut(s) 1475, 1728, 2188, 2307
AsuC2I CCSGG 1 cut(s) 84
AsuHPI GGTGA 4 cut(s) 1135, 1472, 1907, 2057
AsuNHI GCTAGC 2 cut(s) 1231, 1823
AvaI CYCGRG 1 cut(s) 1761
AvaII GGWCC 2 cut(s) 1475, 2307
BbsI GAAGAC 3 cut(s) 1380, 1389, 2171
Bbv12I GWGCWC 2 cut(s) 458, 2354
BbvI GCAGC 4 cut(s) 948, 1088, 1165, 1458
BccI CCATC 7 cut(s) 10, 317, 487, 941, 1788, 1961, 2076
BceAI ACGGC 2 cut(s) 583, 1187
BciT130I CCWGG 3 cut(s) 997, 1877, 2289
BciVI GTATCC 1 cut(s) 490
BclI TGATCA 2 cut(s) 202, 1897
BcnI CCSGG 1 cut(s) 84
BcoDI GTCTC 4 cut(s) 128, 289, 445, 914
BfmI CTRYAG 2 cut(s) 488, 942
BfuI GTATCC 1 cut(s) 490
BisI GCNGC 4 cut(s) 937, 1102, 1179, 1447
BlpI GCTNAGC 1 cut(s) 2353
BlsI GCNGC 4 cut(s) 938, 1103, 1180, 1448
Bme1390I CCNGG 4 cut(s) 84, 997, 1877, 2289
Bme18I GGWCC 2 cut(s) 1475, 2307
BmeT110I CYCGRG 1 cut(s) 1761
BmgT120I GGNCC 4 cut(s) 1475, 1728, 2188, 2307
BmiI GGNNCC 4 cut(s) 753, 1121, 1330, 1950
BmrFI CCNGG 4 cut(s) 84, 997, 1877, 2289
BmrI ACTGGG 1 cut(s) 77
BmsI GCATC 1 cut(s) 1606
BmtI GCTAGC 2 cut(s) 1235, 1827
BmuI ACTGGG 1 cut(s) 77
BpiI GAAGAC 3 cut(s) 1380, 1389, 2171
Bpu1102I GCTNAGC 1 cut(s) 2353
BpuEI CTTGAG 3 cut(s) 456, 1004, 1140
BpuMI CCSGG 1 cut(s) 84
BsaAI YACGTR 1 cut(s) 743
BsaBI GATNNNNATC 1 cut(s) 2067
BsaJI CCNNGG 2 cut(s) 1030, 1366
BsaWI WCCGGW 2 cut(s) 669, 2162
BsaXI ACNNNNNCTCC 2 cut(s) 201, 231
Bsc4I CCNNNNNNNGG 1 cut(s) 473
Bse118I RCCGGY 1 cut(s) 669
Bse1I ACTGG 9 cut(s) 72, 281, 427, 586, 605, 1730, 2130, 2305, 2315
Bse3DI GCAATG 1 cut(s) 708
Bse8I GATNNNNATC 1 cut(s) 2067
BseAI TCCGGA 1 cut(s) 2162
BseBI CCWGG 3 cut(s) 997, 1877, 2289
BseDI CCNNGG 2 cut(s) 1030, 1366
BseGI GGATG 5 cut(s) 251, 643, 914, 2056, 2159
BseJI GATNNNNATC 1 cut(s) 2067
BseLI CCNNNNNNNGG 1 cut(s) 473
BseMI GCAATG 1 cut(s) 708
BseMII CTCAG 4 cut(s) 123, 782, 1330, 2367
BseNI ACTGG 9 cut(s) 72, 281, 427, 586, 605, 1730, 2130, 2305, 2315
BseRI GAGGAG 3 cut(s) 466, 653, 1518
BseXI GCAGC 4 cut(s) 948, 1088, 1165, 1458
BseYI CCCAGC 1 cut(s) 1456
BsgI GTGCAG 1 cut(s) 1926
BshFI GGCC 3 cut(s) 1729, 1875, 2190
BshTI ACCGGT 1 cut(s) 669
BsiHKAI GWGCWC 2 cut(s) 458, 2354
BsiHKCI CYCGRG 1 cut(s) 1761
BsiSI CCGG 3 cut(s) 84, 670, 2163
BslFI GGGAC 2 cut(s) 66, 571
BslI CCNNNNNNNGG 1 cut(s) 473
BsmAI GTCTC 4 cut(s) 128, 289, 445, 914
BsmFI GGGAC 2 cut(s) 66, 571
BsmI GAATGC 2 cut(s) 1278, 1629
BsnI GGCC 3 cut(s) 1729, 1875, 2190
BsoBI CYCGRG 1 cut(s) 1761
Bsp1286I GDGCHC 2 cut(s) 458, 2354
Bsp13I TCCGGA 1 cut(s) 2162
Bsp1407I TGTACA 2 cut(s) 842, 1482
Bsp143I GATC 7 cut(s) 202, 463, 775, 1192, 1245, 1897, 2062
Bsp1720I GCTNAGC 1 cut(s) 2353
BspACI CCGC 2 cut(s) 270, 611
BspANI GGCC 3 cut(s) 1729, 1875, 2190
BspCNI CTCAG 4 cut(s) 124, 781, 1329, 2366
BspEI TCCGGA 1 cut(s) 2162
BspLI GGNNCC 4 cut(s) 753, 1121, 1330, 1950
BspOI GCTAGC 2 cut(s) 1235, 1827
BspPI GGATC 2 cut(s) 458, 1187
BsrDI GCAATG 1 cut(s) 708
BsrFI RCCGGY 1 cut(s) 669
BsrGI TGTACA 2 cut(s) 842, 1482
BsrI ACTGG 9 cut(s) 72, 281, 427, 586, 605, 1730, 2130, 2305, 2315
BssAI RCCGGY 1 cut(s) 669
BssECI CCNNGG 2 cut(s) 1030, 1366
BssMI GATC 7 cut(s) 202, 463, 775, 1192, 1245, 1897, 2062
BssT1I CCWWGG 1 cut(s) 1030
Bst2UI CCWGG 3 cut(s) 997, 1877, 2289
Bst4CI ACNGT 9 cut(s) 139, 244, 717, 965, 1052, 1367, 1401, 1913, 2311
Bst6I CTCTTC 1 cut(s) 1301
BstAUI TGTACA 2 cut(s) 842, 1482
BstBAI YACGTR 1 cut(s) 743
BstC8I GCNNGC 6 cut(s) 389, 934, 1106, 1176, 1233, 1825
BstDEI CTNAG 7 cut(s) 132, 537, 633, 768, 1316, 2353, 2380
BstDSI CCRYGG 1 cut(s) 1366
BstENI CCTNNNNNAGG 1 cut(s) 471
BstF5I GGATG 5 cut(s) 251, 643, 914, 2056, 2159
BstKTI GATC 7 cut(s) 205, 466, 778, 1195, 1248, 1900, 2065
BstMAI GTCTC 4 cut(s) 128, 289, 445, 914
BstMBI GATC 7 cut(s) 202, 463, 775, 1192, 1245, 1897, 2062
BstMWI GCNNNNNNNGC 4 cut(s) 237, 1218, 1726, 2284
BstNI CCWGG 3 cut(s) 997, 1877, 2289
BstNSI RCATGY 5 cut(s) 849, 1669, 1737, 2090, 2233
BstSCI CCNGG 4 cut(s) 82, 995, 1875, 2287
BstSFI CTRYAG 2 cut(s) 488, 942
BstV1I GCAGC 4 cut(s) 948, 1088, 1165, 1458
BstV2I GAAGAC 3 cut(s) 1380, 1389, 2171
BstX2I RGATCY 2 cut(s) 463, 1192
BstYI RGATCY 2 cut(s) 463, 1192
BsuI GTATCC 1 cut(s) 490
BsuRI GGCC 3 cut(s) 1729, 1875, 2190
BtgI CCRYGG 1 cut(s) 1366
BtsCI GGATG 5 cut(s) 251, 643, 914, 2056, 2159
BtsIMutI CAGTG 6 cut(s) 198, 240, 722, 785, 1397, 2298
Cac8I GCNNGC 6 cut(s) 389, 934, 1106, 1176, 1233, 1825
CaiI CAGNNNCTG 1 cut(s) 2321
Cfr10I RCCGGY 1 cut(s) 669
Cfr13I GGNCC 4 cut(s) 1475, 1728, 2188, 2307
CpoI CGGWCCG 1 cut(s) 2307
Csp6I GTAC 9 cut(s) 118, 655, 843, 863, 961, 1059, 1483, 1914, 2012
CspAI ACCGGT 1 cut(s) 669
CspCI CAANNNNNGTGG 2 cut(s) 1905, 1940
CspI CGGWCCG 1 cut(s) 2307
CviQI GTAC 9 cut(s) 118, 655, 843, 863, 961, 1059, 1483, 1914, 2012
DdeI CTNAG 7 cut(s) 132, 537, 633, 768, 1316, 2353, 2380
DpnI GATC 7 cut(s) 204, 465, 777, 1194, 1247, 1899, 2064
DpnII GATC 7 cut(s) 202, 463, 775, 1192, 1245, 1897, 2062
DraI TTTAAA 3 cut(s) 192, 973, 1564
Eam1104I CTCTTC 1 cut(s) 1301
EarI CTCTTC 1 cut(s) 1301
Eco130I CCWWGG 1 cut(s) 1030
Eco32I GATATC 1 cut(s) 587
Eco47I GGWCC 2 cut(s) 1475, 2307
Eco57I CTGAAG 3 cut(s) 1167, 1577, 2235
Eco88I CYCGRG 1 cut(s) 1761
EcoNI CCTNNNNNAGG 1 cut(s) 471
EcoRI GAATTC 2 cut(s) 1139, 1239
EcoRII CCWGG 3 cut(s) 995, 1875, 2287
EcoRV GATATC 1 cut(s) 587
EcoT14I CCWWGG 1 cut(s) 1030
EcoT22I ATGCAT 1 cut(s) 2092
ErhI CCWWGG 1 cut(s) 1030
FaqI GGGAC 2 cut(s) 66, 571
FauNDI CATATG 3 cut(s) 703, 1066, 1936
FbaI TGATCA 2 cut(s) 202, 1897
Fnu4HI GCNGC 4 cut(s) 937, 1102, 1179, 1447
FokI GGATG 5 cut(s) 238, 650, 921, 2043, 2146
Fsp4HI GCNGC 4 cut(s) 937, 1102, 1179, 1447
GluI GCNGC 4 cut(s) 937, 1102, 1179, 1447
GsaI CCCAGC 1 cut(s) 1460
HaeIII GGCC 3 cut(s) 1729, 1875, 2190
HapII CCGG 3 cut(s) 84, 670, 2163
HincII GTYRAC 1 cut(s) 630
HindII GTYRAC 1 cut(s) 630
HindIII AAGCTT 3 cut(s) 401, 1613, 1678
HpaII CCGG 3 cut(s) 84, 670, 2163
HphI GGTGA 4 cut(s) 1135, 1472, 1907, 2057
Hpy166II GTNNAC 4 cut(s) 120, 630, 657, 1483
Hpy8I GTNNAC 4 cut(s) 120, 630, 657, 1483
HpyAV CCTTC 7 cut(s) 230, 523, 1082, 1347, 1457, 1813, 2087
HpyCH4III ACNGT 9 cut(s) 139, 244, 717, 965, 1052, 1367, 1401, 1913, 2311
HpyCH4IV ACGT 3 cut(s) 30, 742, 2138
HpyF10VI GCNNNNNNNGC 4 cut(s) 237, 1218, 1726, 2284
HpyF3I CTNAG 7 cut(s) 132, 537, 633, 768, 1316, 2353, 2380
HpySE526I ACGT 3 cut(s) 30, 742, 2138
Kpn2I TCCGGA 1 cut(s) 2162
Ksp22I TGATCA 2 cut(s) 202, 1897
Kzo9I GATC 7 cut(s) 202, 463, 775, 1192, 1245, 1897, 2062
LmnI GCTCC 4 cut(s) 453, 533, 1328, 1954
Lsp1109I GCAGC 4 cut(s) 948, 1088, 1165, 1458
LweI GCATC 1 cut(s) 1606
MaeII ACGT 3 cut(s) 30, 742, 2138
MaeIII GTNAC 8 cut(s) 3, 196, 418, 827, 965, 1392, 2134, 2342
MalI GATC 7 cut(s) 204, 465, 777, 1194, 1247, 1899, 2064
MboI GATC 7 cut(s) 202, 463, 775, 1192, 1245, 1897, 2062
MflI RGATCY 2 cut(s) 463, 1192
MhlI GDGCHC 2 cut(s) 458, 2354
MlyI GAGTC 3 cut(s) 916, 1781, 2098
MmeI TCCRAC 1 cut(s) 389
Mph1103I ATGCAT 1 cut(s) 2092
MroI TCCGGA 1 cut(s) 2162
MroXI GAANNNNTTC 2 cut(s) 1051, 1647
MseI TTAA 8 cut(s) 171, 191, 578, 695, 972, 1563, 1796, 2073
MspI CCGG 3 cut(s) 84, 670, 2163
MspR9I CCNGG 4 cut(s) 84, 997, 1877, 2289
Mva1269I GAATGC 2 cut(s) 1278, 1629
MvaI CCWGG 3 cut(s) 997, 1877, 2289
MwoI GCNNNNNNNGC 4 cut(s) 237, 1218, 1726, 2284
NciI CCSGG 1 cut(s) 84
NdeI CATATG 3 cut(s) 703, 1066, 1936
NdeII GATC 7 cut(s) 202, 463, 775, 1192, 1245, 1897, 2062
NheI GCTAGC 2 cut(s) 1231, 1823
NlaIV GGNNCC 4 cut(s) 753, 1121, 1330, 1950
NmuCI GTSAC 3 cut(s) 196, 2134, 2342
NsiI ATGCAT 1 cut(s) 2092
NspI RCATGY 5 cut(s) 849, 1669, 1737, 2090, 2233
PaeR7I CTCGAG 1 cut(s) 1761
PctI GAATGC 2 cut(s) 1278, 1629
PdmI GAANNNNTTC 2 cut(s) 1051, 1647
PinAI ACCGGT 1 cut(s) 669
PkrI GCNGC 4 cut(s) 938, 1103, 1180, 1448
PleI GAGTC 3 cut(s) 916, 1781, 2098
PpsI GAGTC 3 cut(s) 916, 1781, 2098
Ppu21I YACGTR 1 cut(s) 743
Psp6I CCWGG 3 cut(s) 995, 1875, 2287
PspFI CCCAGC 1 cut(s) 1456
PspGI CCWGG 3 cut(s) 995, 1875, 2287
PspN4I GGNNCC 4 cut(s) 753, 1121, 1330, 1950
PspPI GGNCC 4 cut(s) 1475, 1728, 2188, 2307
PspXI VCTCGAGB 1 cut(s) 1761
PstNI CAGNNNCTG 1 cut(s) 2321
PsuI RGATCY 2 cut(s) 463, 1192
RsaI GTAC 9 cut(s) 119, 656, 844, 864, 962, 1060, 1484, 1915, 2013
RsaNI GTAC 9 cut(s) 118, 655, 843, 863, 961, 1059, 1483, 1914, 2012
Rsr2I CGGWCCG 1 cut(s) 2307
RsrII CGGWCCG 1 cut(s) 2307
SaqAI TTAA 8 cut(s) 171, 191, 578, 695, 972, 1563, 1796, 2073
SatI GCNGC 4 cut(s) 937, 1102, 1179, 1447
Sau3AI GATC 7 cut(s) 202, 463, 775, 1192, 1245, 1897, 2062
Sau96I GGNCC 4 cut(s) 1475, 1728, 2188, 2307
SchI GAGTC 3 cut(s) 916, 1781, 2098
ScrFI CCNGG 4 cut(s) 84, 997, 1877, 2289
SduI GDGCHC 2 cut(s) 458, 2354
SfaNI GCATC 1 cut(s) 1606
SfcI CTRYAG 2 cut(s) 488, 942
Sfr274I CTCGAG 1 cut(s) 1761
SinI GGWCC 2 cut(s) 1475, 2307
SlaI CTCGAG 1 cut(s) 1761
SmlI CTYRAG 4 cut(s) 471, 1019, 1155, 1761
SmoI CTYRAG 4 cut(s) 471, 1019, 1155, 1761
SsiI CCGC 2 cut(s) 270, 611
StyD4I CCNGG 4 cut(s) 82, 995, 1875, 2287
StyI CCWWGG 1 cut(s) 1030
TaaI ACNGT 9 cut(s) 139, 244, 717, 965, 1052, 1367, 1401, 1913, 2311
TaiI ACGT 3 cut(s) 33, 745, 2141
TaqI TCGA 2 cut(s) 1722, 1762
TatI WGTACW 7 cut(s) 654, 842, 960, 1058, 1482, 1913, 2011
Tru1I TTAA 8 cut(s) 171, 191, 578, 695, 972, 1563, 1796, 2073
Tru9I TTAA 8 cut(s) 171, 191, 578, 695, 972, 1563, 1796, 2073
TscAI CASTG 6 cut(s) 205, 247, 722, 785, 1404, 2305
TseFI GTSAC 3 cut(s) 196, 2134, 2342
TseI GCWGC 4 cut(s) 936, 1101, 1178, 1446
Tsp45I GTSAC 3 cut(s) 196, 2134, 2342
TspGWI ACGGA 2 cut(s) 1318, 2320
TspRI CASTG 6 cut(s) 205, 247, 722, 785, 1404, 2305
VpaK11BI GGWCC 2 cut(s) 1475, 2307
XagI CCTNNNNNAGG 1 cut(s) 471
XapI RAATTY 6 cut(s) 412, 1072, 1139, 1239, 1537, 1647
XbaI TCTAGA 1 cut(s) 1790
XceI RCATGY 5 cut(s) 849, 1669, 1737, 2090, 2233
XcmI CCANNNNNNNNNTGG 1 cut(s) 1339
XhoI CTCGAG 1 cut(s) 1761
XmnI GAANNNNTTC 2 cut(s) 1051, 1647
Zsp2I ATGCAT 1 cut(s) 2092
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.