Rh5BG186900

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000207
Physical Location & Seq
Reverse (-)
20504113 .. 20507807
3695 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG186900.1

Sequence Viewer

Length: 1008 bp
ATGAAGGGAGCTAAGATACTGACTAGAGCAGGGACATGGAACGGCGTTGAATTAACTGGATATCAAGGAAAGCCAAATCCAGTAGCAGTTTTTGAGTTTGTGTTGACTAAGGATGAAGTCTATTATGAGTATACACTCGTCAACAGGTCAATGTTTGCAAGATATGTATTAAACCCATATGGCATTGCACAGTGGCTTACTTGGACAGACCACTCACGTAGTTGGGAACCTTTCATCTCAACTCAGGCAGATCAGTGTGAAAATTATGCCTATTGTGGTGCTAATGCTAGATGTAATGTCAGTAACACTCCTGTATGTACATGCTTGAAAGGGTTTGTACCAAAATCTCCGAAAGATTGGAACTCGACAAATTGGTCTGGGGGATGTGTTCGGGAGACTCCATTAGCTTGCAGCTCTACAGATGGCTTCTCAAAGTACAGTAACTTTAAATTGCCAGACACATCTTCTTCCTGGTATGACAAGAGCATGACCCTCAAGGAGTGCCACGGATTATGTTTGGGAAACTGTTCATGTACTGCATATGCGAATTTAGATGTCAGGGAAGGTGGAAGTGGCTGCTTGCTTTGGTTTGGGAACCTCAATGACATTAGAGAATTCACCTCTGATTCTCAAGACCTCTTTATACGGCTGGCTGCTTCAGACCTAGATCCTATTGTGAAAAAGAGCAAGTCCAGCAAGAAAAAGCTAGCAGGAATTCTGATCGGCTCATCTGTATTTCTTGTGGGAATGCTAATAGTTGGGTTCATTTTGTATAAACGGAAGACGAAACTCAGAAAGCAAGCTCAAATTGACAACTTTGCTGGTGACCGTTGGGTGGAGCTTGCACGCCAACTGTTCGATGTTTTTCCTCACAGAGACTCACCTGTTTCTGCACACAAACCCCATTTCTGGTTTGTGGTAATTAATTCTGAGAAGATTAGTGGTGTTGGGTTTGCGTTTCTTCATTTATATGGGGAATCCAGAAAGCAAAGCTCATCATCTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

335

Amino Acids

37.53

Weight (kDa)

8.9

Isoelectric Point (pI)

36.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S_locus_glycop PF00954 9 - 116 1.4e-24 S-locus glycoprotein domain
PAN_2 PF08276 137 - 203 1.6e-22 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27300
fragaria_vesca FvH4_3g15930 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g21350 FvH4_3g21400 FvH4_3g21400 FvH4_6g12332
malus_domestica MD02G1167100.v1.1 MD02G1167200.v1.1 MD03G1185600.v1.1 MD05G1213900.v1.1 MD05G1214100.v1.1 MD05G1214200.v1.1 MD05G1214700.v1.1 MD05G1216300.v1.1 MD05G1216800.v1.1 MD05G1217300.v1.1 MD05G1218000.v1.1 MD11G1231100.v1.1 MD11G1231200.v1.1 MD11G1231400.v1.1 MD11G1231500.v1.1 MD11G1232400.v1.1 MD11G1232500.v1.1
prunus_persica Prupe.4G142300_v2.0.a1 Prupe.4G142400_v2.0.a1 Prupe.4G142500_v2.0.a1 Prupe.4G142600_v2.0.a1 Prupe.4G142800_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195100_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195500_v2.0.a1 Prupe.4G195700_v2.0.a1 Prupe.4G195900_v2.0.a1
pyrus_communis pycom03g16540 pycom05g19920 pycom11g20460 pycom11g20480 pycom11g20490 pycom11g20500 pycom11g20530
rosa_chinensis RchiOBHm_Chr2g0139311 RchiOBHm_Chr2g0139351 RchiOBHm_Chr5g0026801 RchiOBHm_Chr5g0026851 RchiOBHm_Chr5g0026891 RchiOBHm_Chr5g0026911 RchiOBHm_Chr5g0036571 RchiOBHm_Chr5g0036591 RchiOBHm_Chr5g0036721 RchiOBHm_Chr5g0036741 RchiOBHm_Chr7g0222981
rosa_laevigata RLG00000002006 RLG00000019807 RLG00000032964 RLG00000032968 RLG00000032969 RLG00000033706 RLG00000033708 RLG00000033712 RLG00000033716
rosa_multiflora Rmu_co8256651.1_g000001 Rmu_co8279377.1_g000001 Rmu_co8429919.1_g000001 Rmu_co8490103.1_g000001 Rmu_sc0000774.1_g000002 Rmu_sc0002525.1_g000012 Rmu_sc0004223.1_g000008 Rmu_sc0004390.1_g000002 Rmu_sc0006059.1_g000014 Rmu_sc0006059.1_g000024 Rmu_sc0006514.1_g000020 Rmu_sc0014333.1_g000002 Rmu_ssc0000172.1_g000018 Rmu_ssc0000172.1_g000029
rosa_roxburghii Rroxscaffold_1G00044230 Rroxscaffold_1G00044260 Rroxscaffold_1G00044320 Rroxscaffold_1G00044340 Rroxscaffold_1G00052560 Rroxscaffold_1G00052570 Rroxscaffold_1G00052660 Rroxscaffold_1G00052720 Rroxscaffold_2G00105450 Rroxscaffold_2G00105460
rosa_rugosa Rorug02G0356900 Rorug02G0357000 Rorug02G0357100 Rorug02G0357200 Rorug02G0357300 Rorug02G0357400 Rorug02G0357500 Rorug02G0357600 Rorug05G0095400 Rorug05G0095500 Rorug05G0095500 Rorug05G0095600 Rorug05G0095700 Rorug05G0095800 Rorug05G0157700 Rorug05G0157800
rosa_samantha Rh2AG406800 Rh2AG406900 Rh2BG417300 Rh2CG392900 Rh2DG426200 Rh5AG189300 Rh5AG249400 Rh5AG250100 Rh5BG186300 Rh5BG186900 Rh5BG187000 Rh5BG249500 Rh5BG250600 Rh5BG250900 Rh5BG251500 Rh5BG251800 Rh5CG205500 Rh5CG206300 Rh5CG206700 Rh5CG283000 Rh5CG283300 Rh5CG283500 Rh5DG188500 Rh5DG188700 Rh5DG258700 Rh5DG258900 Rh5DG259600 Rh5DG259900 Rh7CG375800 Rh7DG352500
rosa_wichuraiana Rw2G032960 Rw2G032970 Rw2G033220 Rw2G033230 Rw5G017130 Rw5G017230 Rw5G022940 Rw5G022960 Rw5G022980 Rw7G030420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 373
AccI GTMKAC 1 cut(s) 131
AclWI GGATC 1 cut(s) 662
AcsI RAATTY 3 cut(s) 547, 614, 714
AcuI CTGAAG 1 cut(s) 642
AfaI GTAC 4 cut(s) 319, 339, 437, 535
AfiI CCNNNNNNNGG 2 cut(s) 835, 909
AgsI TTSAA 2 cut(s) 50, 328
AjnI CCWGG 1 cut(s) 470
AluBI AGCT 7 cut(s) 11, 407, 414, 706, 803, 841, 993
AluI AGCT 7 cut(s) 11, 407, 414, 706, 803, 841, 993
Alw26I GTCTC 2 cut(s) 389, 870
AlwI GGATC 1 cut(s) 662
ApeKI GCWGC 3 cut(s) 411, 576, 653
ApoI RAATTY 3 cut(s) 547, 614, 714
AseI ATTAAT 1 cut(s) 924
Asp700I GAANNNNTTC 1 cut(s) 526
AsuHPI GGTGA 3 cut(s) 610, 836, 873
AsuNHI GCTAGC 1 cut(s) 706
BbsI GAAGAC 1 cut(s) 788
BbvI GCAGC 3 cut(s) 423, 563, 640
BccI CCATC 1 cut(s) 416
BceAI ACGGC 2 cut(s) 58, 662
BciT130I CCWGG 1 cut(s) 472
BcoDI GTCTC 2 cut(s) 389, 870
BfaI CTAG 4 cut(s) 24, 288, 665, 707
BfmI CTRYAG 1 cut(s) 417
BisI GCNGC 3 cut(s) 412, 577, 654
BlsI GCNGC 3 cut(s) 413, 578, 655
Bme1390I CCNGG 1 cut(s) 472
BmiI GGNNCC 2 cut(s) 228, 596
BmrFI CCNGG 1 cut(s) 472
BmtI GCTAGC 1 cut(s) 710
BpiI GAAGAC 1 cut(s) 788
BpuEI CTTGAG 2 cut(s) 479, 615
BsaAI YACGTR 1 cut(s) 218
BsaJI CCNNGG 1 cut(s) 505
Bsc4I CCNNNNNNNGG 2 cut(s) 835, 909
Bse1I ACTGG 2 cut(s) 61, 80
Bse3DI GCAATG 1 cut(s) 183
BseBI CCWGG 1 cut(s) 472
BseDI CCNNGG 1 cut(s) 505
BseGI GGATG 2 cut(s) 118, 389
BseLI CCNNNNNNNGG 2 cut(s) 835, 909
BseMI GCAATG 1 cut(s) 183
BseMII CTCAG 3 cut(s) 257, 805, 921
BseNI ACTGG 2 cut(s) 61, 80
BseXI GCAGC 3 cut(s) 423, 563, 640
BsgI GTGCAG 1 cut(s) 876
BslFI GGGAC 1 cut(s) 46
BslI CCNNNNNNNGG 2 cut(s) 835, 909
BsmAI GTCTC 2 cut(s) 389, 870
BsmFI GGGAC 1 cut(s) 46
BsmI GAATGC 1 cut(s) 753
Bsp1407I TGTACA 1 cut(s) 317
Bsp143I GATC 3 cut(s) 250, 667, 720
BspCNI CTCAG 3 cut(s) 256, 804, 922
BspLI GGNNCC 2 cut(s) 228, 596
BspOI GCTAGC 1 cut(s) 710
BspPI GGATC 1 cut(s) 662
BsrDI GCAATG 1 cut(s) 183
BsrGI TGTACA 1 cut(s) 317
BsrI ACTGG 2 cut(s) 61, 80
BssECI CCNNGG 1 cut(s) 505
BssMI GATC 3 cut(s) 250, 667, 720
BssNAI GTATAC 1 cut(s) 132
Bst1107I GTATAC 1 cut(s) 132
Bst2UI CCWGG 1 cut(s) 472
Bst4CI ACNGT 5 cut(s) 192, 440, 527, 830, 855
BstAUI TGTACA 1 cut(s) 317
BstBAI YACGTR 1 cut(s) 218
BstC8I GCNNGC 7 cut(s) 409, 581, 651, 708, 801, 843, 847
BstDEI CTNAG 6 cut(s) 12, 108, 243, 791, 930, 1005
BstDSI CCRYGG 1 cut(s) 505
BstEII GGTNACC 1 cut(s) 824
BstF5I GGATG 2 cut(s) 118, 389
BstKTI GATC 3 cut(s) 253, 670, 723
BstMAI GTCTC 2 cut(s) 389, 870
BstMBI GATC 3 cut(s) 250, 667, 720
BstMWI GCNNNNNNNGC 1 cut(s) 693
BstNI CCWGG 1 cut(s) 472
BstNSI RCATGY 1 cut(s) 324
BstPI GGTNACC 1 cut(s) 824
BstSCI CCNGG 1 cut(s) 470
BstSFI CTRYAG 1 cut(s) 417
BstV1I GCAGC 3 cut(s) 423, 563, 640
BstV2I GAAGAC 1 cut(s) 788
BstX2I RGATCY 1 cut(s) 667
BstYI RGATCY 1 cut(s) 667
BstZ17I GTATAC 1 cut(s) 132
BtgI CCRYGG 1 cut(s) 505
BtsCI GGATG 2 cut(s) 118, 389
BtsIMutI CAGTG 2 cut(s) 197, 260
Cac8I GCNNGC 7 cut(s) 409, 581, 651, 708, 801, 843, 847
Csp6I GTAC 4 cut(s) 318, 338, 436, 534
CviAII CATG 4 cut(s) 36, 321, 487, 531
CviQI GTAC 4 cut(s) 318, 338, 436, 534
DdeI CTNAG 6 cut(s) 12, 108, 243, 791, 930, 1005
DpnI GATC 3 cut(s) 252, 669, 722
DpnII GATC 3 cut(s) 250, 667, 720
DraI TTTAAA 1 cut(s) 448
DrdI GACNNNNNNGTC 1 cut(s) 373
DseDI GACNNNNNNGTC 1 cut(s) 373
Eco32I GATATC 1 cut(s) 62
Eco57I CTGAAG 1 cut(s) 642
Eco91I GGTNACC 1 cut(s) 824
EcoO65I GGTNACC 1 cut(s) 824
EcoRI GAATTC 2 cut(s) 614, 714
EcoRII CCWGG 1 cut(s) 470
EcoRV GATATC 1 cut(s) 62
FaeI CATG 4 cut(s) 39, 324, 490, 534
FaqI GGGAC 1 cut(s) 46
FatI CATG 4 cut(s) 35, 320, 486, 530
FauNDI CATATG 2 cut(s) 178, 541
FblI GTMKAC 1 cut(s) 131
Fnu4HI GCNGC 3 cut(s) 412, 577, 654
FokI GGATG 2 cut(s) 125, 396
Fsp4HI GCNGC 3 cut(s) 412, 577, 654
FspBI CTAG 4 cut(s) 24, 288, 665, 707
GluI GCNGC 3 cut(s) 412, 577, 654
Hin1II CATG 4 cut(s) 39, 324, 490, 534
HincII GTYRAC 2 cut(s) 105, 142
HindII GTYRAC 2 cut(s) 105, 142
HinfI GANTC 4 cut(s) 397, 626, 878, 977
HphI GGTGA 3 cut(s) 610, 836, 873
Hpy166II GTNNAC 3 cut(s) 105, 132, 142
Hpy188I TCNGA 6 cut(s) 351, 625, 661, 720, 794, 931
Hpy188III TCNNGA 3 cut(s) 392, 632, 981
Hpy8I GTNNAC 3 cut(s) 105, 132, 142
HpyAV CCTTC 1 cut(s) 557
HpyCH4III ACNGT 5 cut(s) 192, 440, 527, 830, 855
HpyCH4IV ACGT 1 cut(s) 217
HpyCH4V TGCA 6 cut(s) 158, 188, 411, 539, 845, 893
HpyF10VI GCNNNNNNNGC 1 cut(s) 693
HpyF3I CTNAG 6 cut(s) 12, 108, 243, 791, 930, 1005
HpySE526I ACGT 1 cut(s) 217
Hsp92II CATG 4 cut(s) 39, 324, 490, 534
Kzo9I GATC 3 cut(s) 250, 667, 720
LmnI GCTCC 2 cut(s) 8, 838
Lsp1109I GCAGC 3 cut(s) 423, 563, 640
MaeI CTAG 4 cut(s) 24, 288, 665, 707
MaeII ACGT 1 cut(s) 217
MaeIII GTNAC 3 cut(s) 302, 440, 824
MalI GATC 3 cut(s) 252, 669, 722
MboI GATC 3 cut(s) 250, 667, 720
MboII GAAGA 6 cut(s) 456, 459, 793, 946, 953, 993
MflI RGATCY 1 cut(s) 667
MlyI GAGTC 2 cut(s) 391, 872
MnlI CCTC 5 cut(s) 503, 608, 631, 647, 879
MroXI GAANNNNTTC 1 cut(s) 526
MseI TTAA 4 cut(s) 53, 170, 447, 924
MslI CAYNNNNRTG 1 cut(s) 969
MspR9I CCNGG 1 cut(s) 472
Mva1269I GAATGC 1 cut(s) 753
MvaI CCWGG 1 cut(s) 472
MwoI GCNNNNNNNGC 1 cut(s) 693
NdeI CATATG 2 cut(s) 178, 541
NdeII GATC 3 cut(s) 250, 667, 720
NheI GCTAGC 1 cut(s) 706
NlaIII CATG 4 cut(s) 39, 324, 490, 534
NlaIV GGNNCC 2 cut(s) 228, 596
NmuCI GTSAC 1 cut(s) 824
NspI RCATGY 1 cut(s) 324
PctI GAATGC 1 cut(s) 753
PdmI GAANNNNTTC 1 cut(s) 526
PfeI GAWTC 2 cut(s) 626, 977
PkrI GCNGC 3 cut(s) 413, 578, 655
PleI GAGTC 2 cut(s) 391, 872
PpsI GAGTC 2 cut(s) 391, 872
Ppu21I YACGTR 1 cut(s) 218
PshBI ATTAAT 1 cut(s) 924
Psp6I CCWGG 1 cut(s) 470
PspEI GGTNACC 1 cut(s) 824
PspGI CCWGG 1 cut(s) 470
PspN4I GGNNCC 2 cut(s) 228, 596
PsuI RGATCY 1 cut(s) 667
RsaI GTAC 4 cut(s) 319, 339, 437, 535
RsaNI GTAC 4 cut(s) 318, 338, 436, 534
RseI CAYNNNNRTG 1 cut(s) 969
SaqAI TTAA 4 cut(s) 53, 170, 447, 924
SatI GCNGC 3 cut(s) 412, 577, 654
Sau3AI GATC 3 cut(s) 250, 667, 720
SchI GAGTC 2 cut(s) 391, 872
ScrFI CCNGG 1 cut(s) 472
SfcI CTRYAG 1 cut(s) 417
SmiMI CAYNNNNRTG 1 cut(s) 969
SmlI CTYRAG 2 cut(s) 494, 630
SmoI CTYRAG 2 cut(s) 494, 630
SspMI CTAG 4 cut(s) 24, 288, 665, 707
StyD4I CCNGG 1 cut(s) 470
TaaI ACNGT 5 cut(s) 192, 440, 527, 830, 855
TaiI ACGT 1 cut(s) 220
TaqI TCGA 2 cut(s) 365, 858
TatI WGTACW 3 cut(s) 317, 435, 533
TfiI GAWTC 2 cut(s) 626, 977
Tru1I TTAA 4 cut(s) 53, 170, 447, 924
Tru9I TTAA 4 cut(s) 53, 170, 447, 924
TscAI CASTG 2 cut(s) 197, 260
TseFI GTSAC 1 cut(s) 824
TseI GCWGC 3 cut(s) 411, 576, 653
Tsp45I GTSAC 1 cut(s) 824
TspDTI ATGAA 6 cut(s) 17, 129, 223, 519, 754, 953
TspGWI ACGGA 2 cut(s) 522, 793
TspRI CASTG 2 cut(s) 197, 260
VspI ATTAAT 1 cut(s) 924
XapI RAATTY 3 cut(s) 547, 614, 714
XceI RCATGY 1 cut(s) 324
XmiI GTMKAC 1 cut(s) 131
XmnI GAANNNNTTC 1 cut(s) 526
XspI CTAG 4 cut(s) 24, 288, 665, 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.