Prupe.4G142400_v2.0.a1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000207
Physical Location & Seq
Reverse (-)
8166460 .. 8175813
9354 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G142400.1

Sequence Viewer

Length: 2472 bp
ATGCAAGCCTTGAGGAACCTTTTTGTGTGCTTCTTGTTTTTCTCCTTCTTAAGAATTTCAACTACAACCACACTAGACACAATCACTCCAAGTAGATATATTAGAGATGGTGAAACTCTAGTTTCTGCAGGTGGAAGCTTTGAACTTGGATTCTTTAGCCCCGGTGCATCACAAGGCCGATACTTGGGAATATGGTACACTTTTGATACTGAGGCAGTTGTATGGGTAGCCAACAGAGAAATACCACTTGGCAATTCTTCAGGAGTTTTGAAGGTCACCGAGCAAGGAGTTCTAGTCCTTCTCAATAGCTCAAACAGGATTGCATGGTCATCAAATTCATCAACAACTGCAGGAAATCCAGTGTCACAACTCTTGGATTCTGGAAATCTTGTTGTGAAAGATGGAAATGAAACTAACCCTGATAAATTCTTGTGGCAGAGTTTTGATTATCCTTGTGACACATTCCTACCAGAAATGAAGCTTGGTTGGGACTTAGTTACTGGTTTAGATAGGTACGTCTCGTCTTGGAGGAGCACAGAAGATCCTGCTCCAGGAGAGTTTTCATTACGGATGGATCGTCGTGGTTTCCCACAAGTTGATACTATGAAAGGAGCTAAGATAATGGCTACAGCGGGGTCATGGAACGGCATTTATTTTACAGCATATCCATATCCTCCACAAACACAGGAAAACCAAATACTTGGGCATGAATTTGTGTTGGACAAGGATGAGATCTATTACGAGTACAGACTCCTAAACAGGTCCACGTTCTCAAGATACGTACTGAATCCATCAGGCATTGCACAGAGATTCACATGGGTGCACCAAACACATAGTTGGGAACTTTCCTCTACATTCCAAGCAGATCAGTGTCAAAATTATGCCTTGTGTGGCGCATATACTAGTTGCAATATGAATGTCTCTCCCTCATGTGAATGCTTGAAGGGATTTGTTCCAAAATCTCCACAAGATTGGAATTCGGGATATTGGTCTGATGGGTGTGTTCGAAAGATTCCATTGGCTTGCAGCTATGGAGATGGCTTCCTAAAGTACACTGGTGTTAAATTGCCAGACACATCTTCCTCATGGTTTGATGAGAGCATGAGCCTCAAGGAATGTAAGGGATTATGTTTGAAAAACTGCTCATGTACGGCATATGCAAATTTAGATGTCAGGGAGGGAGGAACTGGCTGTTTGCTTTGGTTTGGTAACCTCACTGACATAAGACAATTCACTCCCGGTGGTGGTCAAGACCTCTATATACGGATGGCAGCTTCAGAACTTGATGGAATTGAGAAAAAAAGCAAGTTCAACAAGGAAAAGCTACCTGGAATTCTTATCAGCTCTGCAGTTTTTCTTGTCGGAACGTTAATAATTGGATTGATATTGTATATACAGAAGAAGAAACTCAGAAATCAAGGAGTCAGAAGCATAGATTGCAGAAAAGATTACCTTGGAGAAGACAGGGAAAACATGGAGTTACCACTATTTGATTTGAGCACCATAGCTAAAGCCACCAATGACTTTTCAAGCAGCAACAAGCTGGGAGAAGGTGGTTTTGGACCTGTGTATAAGGGTACATTGATAGGAGGGAAAGAAATTGCAGTAAAGAGGCTTTCAAAGGATTCTGGACAAGGAATGAGGGAGTTCAAAAATGAAGTTATACTGATAGCCAAACTTCAGCACCGCAATCTTGTAAAGCTTCTTGGTTGTTGCACTCAAGATGACGAAAAAATCTTAATATATGAATTCATGGCCAACAGAAGTTTGGATTTCTATATTTTTGACCAGGGAAGAGCGAAATTGCTCGACTGGCCTAAGTGCTTCCACATTATTGATGGAATTGCTCGAGGACTTCTTTATCTTCACCAAGACTCTAGATTAAGGATTATCCATAGAGATCTGAAAGCTAGCAATATTTTGCTTGATAATGATATGAACCCAAAGATTTCTGACTTTGGCCTGGCCAGAATATTTGGTGCCGATCAAAGTCGGGGTAATACAAAAAGAGTGGTTGGAACATATGGCTATATGCCCCCTGAATATGCAGTTGATGGAATTTTCTCGACGAAATCAGATGTATTTAGCTTTGGTGTTATACTGCTAGAGATATTGAGTAGGCAGAAGAACAGGGGATTTTGGCATCCAGATCACCATCTTAACCTTCTTGGACATGCATGGACACTATGGATCCAAAATATTCCACTGGAACTGATTGATAAGTCGCTAAGCGATTCGTGCACCATATCCGAAGTATTACGGTGTCTTCATGTGGCTCTGTTATGCGTGCAACAAGTACCTGAAGATAGACCAAGCATGTCATCTGTGGTTCTAATGCTGAGCAGTGAGGTTGCATTGCCCCCACCAAAGCAACCTGGTTTTTACACCGAACGAACTCTACCTGATGATCCGTCAAGGGTGCGTGATTTGTCGGAAAATAATTTCAGCACTACATTGTTAGAGGCTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

824

Amino Acids

92.43

Weight (kDa)

6.36

Isoelectric Point (pI)

42.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27300
fragaria_vesca FvH4_3g15930 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g21350 FvH4_3g21400 FvH4_3g21400 FvH4_6g12332
malus_domestica MD02G1167100.v1.1 MD02G1167200.v1.1 MD03G1185600.v1.1 MD05G1213900.v1.1 MD05G1214100.v1.1 MD05G1214200.v1.1 MD05G1214700.v1.1 MD05G1216300.v1.1 MD05G1216800.v1.1 MD05G1217300.v1.1 MD05G1218000.v1.1 MD11G1231100.v1.1 MD11G1231200.v1.1 MD11G1231400.v1.1 MD11G1231500.v1.1 MD11G1232400.v1.1 MD11G1232500.v1.1
prunus_persica Prupe.4G142300_v2.0.a1 Prupe.4G142400_v2.0.a1 Prupe.4G142500_v2.0.a1 Prupe.4G142600_v2.0.a1 Prupe.4G142800_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195100_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195500_v2.0.a1 Prupe.4G195700_v2.0.a1 Prupe.4G195900_v2.0.a1
pyrus_communis pycom03g16540 pycom05g19920 pycom11g20460 pycom11g20480 pycom11g20490 pycom11g20500 pycom11g20530
rosa_chinensis RchiOBHm_Chr2g0139311 RchiOBHm_Chr2g0139351 RchiOBHm_Chr5g0026801 RchiOBHm_Chr5g0026851 RchiOBHm_Chr5g0026891 RchiOBHm_Chr5g0026911 RchiOBHm_Chr5g0036571 RchiOBHm_Chr5g0036591 RchiOBHm_Chr5g0036721 RchiOBHm_Chr5g0036741 RchiOBHm_Chr7g0222981
rosa_laevigata RLG00000002006 RLG00000019807 RLG00000032964 RLG00000032968 RLG00000032969 RLG00000033706 RLG00000033708 RLG00000033712 RLG00000033716
rosa_multiflora Rmu_co8256651.1_g000001 Rmu_co8279377.1_g000001 Rmu_co8429919.1_g000001 Rmu_co8490103.1_g000001 Rmu_sc0000774.1_g000002 Rmu_sc0002525.1_g000012 Rmu_sc0004223.1_g000008 Rmu_sc0004390.1_g000002 Rmu_sc0006059.1_g000014 Rmu_sc0006059.1_g000024 Rmu_sc0006514.1_g000020 Rmu_sc0014333.1_g000002 Rmu_ssc0000172.1_g000018 Rmu_ssc0000172.1_g000029
rosa_roxburghii Rroxscaffold_1G00044230 Rroxscaffold_1G00044260 Rroxscaffold_1G00044320 Rroxscaffold_1G00044340 Rroxscaffold_1G00052560 Rroxscaffold_1G00052570 Rroxscaffold_1G00052660 Rroxscaffold_1G00052720 Rroxscaffold_2G00105450 Rroxscaffold_2G00105460
rosa_rugosa Rorug02G0356900 Rorug02G0357000 Rorug02G0357100 Rorug02G0357200 Rorug02G0357300 Rorug02G0357400 Rorug02G0357500 Rorug02G0357600 Rorug05G0095400 Rorug05G0095500 Rorug05G0095500 Rorug05G0095600 Rorug05G0095700 Rorug05G0095800 Rorug05G0157700 Rorug05G0157800
rosa_samantha Rh2AG406800 Rh2AG406900 Rh2BG417300 Rh2CG392900 Rh2DG426200 Rh5AG189300 Rh5AG249400 Rh5AG250100 Rh5BG186300 Rh5BG186900 Rh5BG187000 Rh5BG249500 Rh5BG250600 Rh5BG250900 Rh5BG251500 Rh5BG251800 Rh5CG205500 Rh5CG206300 Rh5CG206700 Rh5CG283000 Rh5CG283300 Rh5CG283500 Rh5DG188500 Rh5DG188700 Rh5DG258700 Rh5DG258900 Rh5DG259600 Rh5DG259900 Rh7CG375800 Rh7DG352500
rosa_wichuraiana Rw2G032960 Rw2G032970 Rw2G033220 Rw2G033230 Rw5G017130 Rw5G017230 Rw5G022940 Rw5G022960 Rw5G022980 Rw7G030420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 119
Acc36I ACCTGC 1 cut(s) 119
AccB1I GGYRCC 1 cut(s) 1979
AciI CCGC 2 cut(s) 632, 1687
AclI AACGTT 1 cut(s) 1367
AclWI GGATC 5 cut(s) 536, 582, 2185, 2198, 2402
AcoI YGGCCR 2 cut(s) 1755, 1965
AcsI RAATTY 9 cut(s) 54, 334, 425, 710, 976, 1162, 1332, 1748, 2058
AcuI CTGAAG 4 cut(s) 243, 1260, 1664, 2322
AfaI GTAC 8 cut(s) 197, 515, 746, 783, 1052, 1150, 1579, 2298
AfiI CCNNNNNNNGG 3 cut(s) 184, 551, 1244
AflII CTTAAG 1 cut(s) 49
AgsI TTSAA 9 cut(s) 60, 143, 271, 943, 1135, 1312, 1530, 1620, 1651
AhlI ACTAGT 1 cut(s) 902
AjnI CCWGG 5 cut(s) 550, 1327, 1788, 1962, 2374
AjuI GAANNNNNNNTTGG 6 cut(s) 231, 263, 465, 497, 1542, 1574
AleI CACNNNNGTG 1 cut(s) 818
Alw21I GWGCWC 4 cut(s) 536, 825, 1502, 2243
Alw26I GTCTC 2 cut(s) 523, 925
Alw44I GTGCAC 2 cut(s) 821, 2239
AlwI GGATC 5 cut(s) 536, 582, 2185, 2198, 2402
Ama87I CYCGRG 1 cut(s) 1848
AoxI GGCC 5 cut(s) 175, 1755, 1814, 1960, 1965
ApaLI GTGCAC 2 cut(s) 821, 2239
ApeKI GCWGC 3 cut(s) 1026, 1271, 1533
ApoI RAATTY 9 cut(s) 54, 334, 425, 710, 976, 1162, 1332, 1748, 2058
AspLEI GCGC 1 cut(s) 896
AspS9I GGNCC 2 cut(s) 762, 1562
AsuC2I CCSGG 2 cut(s) 162, 1239
AsuHPI GGTGA 4 cut(s) 122, 268, 1859, 2144
AsuII TTCGAA 1 cut(s) 1006
AsuNHI GCTAGC 1 cut(s) 1910
AvaI CYCGRG 1 cut(s) 1848
AvaII GGWCC 2 cut(s) 762, 1562
BaeGI GKGCMC 2 cut(s) 825, 2243
BalI TGGCCA 2 cut(s) 1757, 1967
BamHI GGATCC 1 cut(s) 2190
BanI GGYRCC 1 cut(s) 1979
BbsI GAAGAC 2 cut(s) 1467, 2258
Bbv12I GWGCWC 4 cut(s) 536, 825, 1502, 2243
BbvI GCAGC 3 cut(s) 1038, 1283, 1545
BceAI ACGGC 2 cut(s) 661, 1167
BciT130I CCWGG 5 cut(s) 552, 1329, 1790, 1964, 2376
BcnI CCSGG 2 cut(s) 162, 1239
BcoDI GTCTC 2 cut(s) 523, 925
BcuI ACTAGT 1 cut(s) 902
BfaI CTAG 7 cut(s) 74, 119, 293, 903, 1878, 1911, 2105
BfmI CTRYAG 4 cut(s) 126, 348, 627, 1347
BfrI CTTAAG 1 cut(s) 49
BfuAI ACCTGC 1 cut(s) 119
BglII AGATCT 2 cut(s) 732, 1900
BisI GCNGC 3 cut(s) 1027, 1272, 1534
BlpI GCTNAGC 2 cut(s) 2228, 2339
BlsI GCNGC 3 cut(s) 1028, 1273, 1535
Bme1390I CCNGG 7 cut(s) 162, 552, 1239, 1329, 1790, 1964, 2376
Bme18I GGWCC 2 cut(s) 762, 1562
BmeT110I CYCGRG 1 cut(s) 1848
BmgT120I GGNCC 2 cut(s) 762, 1562
BmiI GGNNCC 3 cut(s) 17, 1981, 2192
BmrFI CCNGG 7 cut(s) 162, 552, 1239, 1329, 1790, 1964, 2376
BmsI GCATC 2 cut(s) 176, 2152
BmtI GCTAGC 1 cut(s) 1914
BpiI GAAGAC 2 cut(s) 1467, 2258
BpmI CTGGAG 1 cut(s) 534
Bpu1102I GCTNAGC 2 cut(s) 2228, 2339
Bpu14I TTCGAA 1 cut(s) 1006
BpuEI CTTGAG 4 cut(s) 31, 757, 1094, 1704
BpuMI CCSGG 2 cut(s) 162, 1239
BsaAI YACGTR 1 cut(s) 781
BsaBI GATNNNNATC 1 cut(s) 2154
BsaJI CCNNGG 3 cut(s) 160, 1453, 1789
BsaXI ACNNNNNCTCC 4 cut(s) 70, 100, 279, 309
Bsc4I CCNNNNNNNGG 3 cut(s) 184, 551, 1244
Bse1I ACTGG 6 cut(s) 359, 505, 1060, 1192, 1817, 2211
Bse3DI GCAATG 2 cut(s) 798, 2354
Bse8I GATNNNNATC 1 cut(s) 2154
BseBI CCWGG 5 cut(s) 552, 1329, 1790, 1964, 2376
BseDI CCNNGG 3 cut(s) 160, 1453, 1789
BseGI GGATG 4 cut(s) 576, 733, 1272, 2143
BseJI GATNNNNATC 1 cut(s) 2154
BseLI CCNNNNNNNGG 3 cut(s) 184, 551, 1244
BseMI GCAATG 2 cut(s) 798, 2354
BseMII CTCAG 3 cut(s) 201, 1423, 2330
BseNI ACTGG 6 cut(s) 359, 505, 1060, 1192, 1817, 2211
BseRI GAGGAG 1 cut(s) 544
BseSI GKGCMC 2 cut(s) 825, 2243
BseXI GCAGC 3 cut(s) 1038, 1283, 1545
BseYI CCCAGC 1 cut(s) 1543
BshFI GGCC 5 cut(s) 177, 1757, 1816, 1962, 1967
BshNI GGYRCC 1 cut(s) 1979
BsiHKAI GWGCWC 4 cut(s) 536, 825, 1502, 2243
BsiHKCI CYCGRG 1 cut(s) 1848
BsiSI CCGG 2 cut(s) 162, 1239
BslFI GGGAC 1 cut(s) 503
BslI CCNNNNNNNGG 3 cut(s) 184, 551, 1244
BsmAI GTCTC 2 cut(s) 523, 925
BsmBI CGTCTC 1 cut(s) 523
BsmFI GGGAC 1 cut(s) 503
BsmI GAATGC 1 cut(s) 941
BsnI GGCC 5 cut(s) 177, 1757, 1816, 1962, 1967
BsoBI CYCGRG 1 cut(s) 1848
Bsp119I TTCGAA 1 cut(s) 1006
Bsp1286I GDGCHC 4 cut(s) 536, 825, 1502, 2243
Bsp143I GATC 9 cut(s) 541, 574, 732, 865, 1900, 1984, 2149, 2190, 2407
Bsp1720I GCTNAGC 2 cut(s) 2228, 2339
BspACI CCGC 2 cut(s) 632, 1687
BspANI GGCC 5 cut(s) 177, 1757, 1816, 1962, 1967
BspCNI CTCAG 3 cut(s) 202, 1422, 2331
BspLI GGNNCC 3 cut(s) 17, 1981, 2192
BspMAI CTGCAG 3 cut(s) 130, 352, 1351
BspMI ACCTGC 1 cut(s) 119
BspOI GCTAGC 1 cut(s) 1914
BspPI GGATC 5 cut(s) 536, 582, 2185, 2198, 2402
BspQI GCTCTTC 1 cut(s) 1789
BspT104I TTCGAA 1 cut(s) 1006
BspT107I GGYRCC 1 cut(s) 1979
BspTI CTTAAG 1 cut(s) 49
BsrDI GCAATG 2 cut(s) 798, 2354
BsrI ACTGG 6 cut(s) 359, 505, 1060, 1192, 1817, 2211
BssECI CCNNGG 3 cut(s) 160, 1453, 1789
BssMI GATC 9 cut(s) 541, 574, 732, 865, 1900, 1984, 2149, 2190, 2407
BssT1I CCWWGG 1 cut(s) 1453
Bst2UI CCWGG 5 cut(s) 552, 1329, 1790, 1964, 2376
Bst4CI ACNGT 1 cut(s) 2262
Bst6I CTCTTC 1 cut(s) 1789
BstAFI CTTAAG 1 cut(s) 49
BstAPI GCANNNNNTGC 1 cut(s) 1437
BstBAI YACGTR 1 cut(s) 781
BstBI TTCGAA 1 cut(s) 1006
BstC8I GCNNGC 4 cut(s) 6, 1024, 1912, 2288
BstDEI CTNAG 7 cut(s) 210, 493, 615, 1409, 1818, 2228, 2339
BstEII GGTNACC 2 cut(s) 274, 1208
BstENI CCTNNNNNAGG 1 cut(s) 549
BstF5I GGATG 4 cut(s) 576, 733, 1272, 2143
BstHHI GCGC 1 cut(s) 896
BstKTI GATC 9 cut(s) 544, 577, 735, 868, 1903, 1987, 2152, 2193, 2410
BstMAI GTCTC 2 cut(s) 523, 925
BstMBI GATC 9 cut(s) 541, 574, 732, 865, 1900, 1984, 2149, 2190, 2407
BstMWI GCNNNNNNNGC 3 cut(s) 1437, 1813, 2238
BstNI CCWGG 5 cut(s) 552, 1329, 1790, 1964, 2376
BstNSI RCATGY 2 cut(s) 2177, 2320
BstPI GGTNACC 2 cut(s) 274, 1208
BstSCI CCNGG 7 cut(s) 160, 550, 1237, 1327, 1788, 1962, 2374
BstSFI CTRYAG 4 cut(s) 126, 348, 627, 1347
BstSLI GKGCMC 2 cut(s) 825, 2243
BstSNI TACGTA 1 cut(s) 781
BstV1I GCAGC 3 cut(s) 1038, 1283, 1545
BstV2I GAAGAC 2 cut(s) 1467, 2258
BstX2I RGATCY 4 cut(s) 541, 732, 1900, 2190
BstXI CCANNNNNNTGG 2 cut(s) 701, 972
BstYI RGATCY 4 cut(s) 541, 732, 1900, 2190
BsuRI GGCC 5 cut(s) 177, 1757, 1816, 1962, 1967
BtsCI GGATG 4 cut(s) 576, 733, 1272, 2143
BtsI GCAGTG 1 cut(s) 2350
BtsIMutI CAGTG 6 cut(s) 366, 875, 1053, 1215, 2204, 2350
BveI ACCTGC 1 cut(s) 119
Cac8I GCNNGC 4 cut(s) 6, 1024, 1912, 2288
CfoI GCGC 1 cut(s) 896
Cfr13I GGNCC 2 cut(s) 762, 1562
CsiI ACCWGGT 1 cut(s) 2374
Csp6I GTAC 8 cut(s) 196, 514, 745, 782, 1051, 1149, 1578, 2297
CspCI CAANNNNNGTGG 2 cut(s) 1992, 2027
CviQI GTAC 8 cut(s) 196, 514, 745, 782, 1051, 1149, 1578, 2297
DdeI CTNAG 7 cut(s) 210, 493, 615, 1409, 1818, 2228, 2339
DpnI GATC 9 cut(s) 543, 576, 734, 867, 1902, 1986, 2151, 2192, 2409
DpnII GATC 9 cut(s) 541, 574, 732, 865, 1900, 1984, 2149, 2190, 2407
EaeI YGGCCR 2 cut(s) 1755, 1965
Eam1104I CTCTTC 1 cut(s) 1789
EarI CTCTTC 1 cut(s) 1789
Eco105I TACGTA 1 cut(s) 781
Eco130I CCWWGG 1 cut(s) 1453
Eco47I GGWCC 2 cut(s) 762, 1562
Eco57I CTGAAG 4 cut(s) 243, 1260, 1664, 2322
Eco88I CYCGRG 1 cut(s) 1848
Eco91I GGTNACC 2 cut(s) 274, 1208
EcoNI CCTNNNNNAGG 1 cut(s) 549
EcoO65I GGTNACC 2 cut(s) 274, 1208
EcoRI GAATTC 3 cut(s) 976, 1332, 1748
EcoRII CCWGG 5 cut(s) 550, 1327, 1788, 1962, 2374
EcoT14I CCWWGG 1 cut(s) 1453
EcoT22I ATGCAT 1 cut(s) 2179
ErhI CCWWGG 1 cut(s) 1453
Esp3I CGTCTC 1 cut(s) 523
FalI AAGNNNNNCTT 2 cut(s) 1437, 1469
FaqI GGGAC 1 cut(s) 503
FauI CCCGC 1 cut(s) 625
FauNDI CATATG 2 cut(s) 1156, 2023
Fnu4HI GCNGC 3 cut(s) 1027, 1272, 1534
FokI GGATG 4 cut(s) 583, 740, 1279, 2130
Fsp4HI GCNGC 3 cut(s) 1027, 1272, 1534
FspBI CTAG 7 cut(s) 74, 119, 293, 903, 1878, 1911, 2105
GlaI GCGC 1 cut(s) 895
GluI GCNGC 3 cut(s) 1027, 1272, 1534
GsaI CCCAGC 1 cut(s) 1547
GsuI CTGGAG 1 cut(s) 534
HaeIII GGCC 5 cut(s) 177, 1757, 1816, 1962, 1967
HapII CCGG 2 cut(s) 162, 1239
HhaI GCGC 1 cut(s) 896
Hin6I GCGC 1 cut(s) 894
HinP1I GCGC 1 cut(s) 894
HindIII AAGCTT 3 cut(s) 136, 479, 1700
HpaII CCGG 2 cut(s) 162, 1239
HphI GGTGA 4 cut(s) 122, 268, 1859, 2144
Hpy166II GTNNAC 5 cut(s) 198, 765, 823, 1053, 2241
Hpy8I GTNNAC 5 cut(s) 198, 765, 823, 1053, 2241
Hpy99I CGWCG 2 cut(s) 582, 2071
HpyAV CCTTC 6 cut(s) 55, 265, 308, 937, 1544, 2174
HpyCH4III ACNGT 1 cut(s) 2262
HpyCH4IV ACGT 4 cut(s) 516, 767, 780, 1367
HpyF10VI GCNNNNNNNGC 3 cut(s) 1437, 1813, 2238
HpyF3I CTNAG 7 cut(s) 210, 493, 615, 1409, 1818, 2228, 2339
HpySE526I ACGT 4 cut(s) 516, 767, 780, 1367
HspAI GCGC 1 cut(s) 894
Kzo9I GATC 9 cut(s) 541, 574, 732, 865, 1900, 1984, 2149, 2190, 2407
LguI GCTCTTC 1 cut(s) 1789
LmnI GCTCC 3 cut(s) 531, 553, 611
Lsp1109I GCAGC 3 cut(s) 1038, 1283, 1545
LweI GCATC 2 cut(s) 176, 2152
MabI ACCWGGT 1 cut(s) 2374
MaeI CTAG 7 cut(s) 74, 119, 293, 903, 1878, 1911, 2105
MaeII ACGT 4 cut(s) 516, 767, 780, 1367
MaeIII GTNAC 6 cut(s) 274, 363, 455, 496, 1208, 1479
MalI GATC 9 cut(s) 543, 576, 734, 867, 1902, 1986, 2151, 2192, 2409
MboI GATC 9 cut(s) 541, 574, 732, 865, 1900, 1984, 2149, 2190, 2407
MflI RGATCY 4 cut(s) 541, 732, 1900, 2190
MhlI GDGCHC 4 cut(s) 536, 825, 1502, 2243
MlsI TGGCCA 2 cut(s) 1757, 1967
MluNI TGGCCA 2 cut(s) 1757, 1967
MlyI GAGTC 3 cut(s) 744, 1431, 1868
MmeI TCCRAC 4 cut(s) 699, 1342, 1996, 2412
Mox20I TGGCCA 2 cut(s) 1757, 1967
Mph1103I ATGCAT 1 cut(s) 2179
MscI TGGCCA 2 cut(s) 1757, 1967
MseI TTAA 6 cut(s) 50, 1062, 1370, 1739, 1883, 2160
MslI CAYNNNNRTG 2 cut(s) 818, 934
Msp20I TGGCCA 2 cut(s) 1757, 1967
MspA1I CMGCKG 1 cut(s) 632
MspCI CTTAAG 1 cut(s) 49
MspI CCGG 2 cut(s) 162, 1239
MspR9I CCNGG 7 cut(s) 162, 552, 1239, 1329, 1790, 1964, 2376
Mva1269I GAATGC 1 cut(s) 941
MvaI CCWGG 5 cut(s) 552, 1329, 1790, 1964, 2376
MwoI GCNNNNNNNGC 3 cut(s) 1437, 1813, 2238
NciI CCSGG 2 cut(s) 162, 1239
NdeI CATATG 2 cut(s) 1156, 2023
NdeII GATC 9 cut(s) 541, 574, 732, 865, 1900, 1984, 2149, 2190, 2407
NheI GCTAGC 1 cut(s) 1910
NlaIV GGNNCC 3 cut(s) 17, 1981, 2192
NmuCI GTSAC 3 cut(s) 274, 363, 455
NsiI ATGCAT 1 cut(s) 2179
NspI RCATGY 2 cut(s) 2177, 2320
NspV TTCGAA 1 cut(s) 1006
OliI CACNNNNGTG 1 cut(s) 818
PaeR7I CTCGAG 1 cut(s) 1848
PaqCI CACCTGC 1 cut(s) 119
PciSI GCTCTTC 1 cut(s) 1789
PcsI WCGNNNNNNNCGW 1 cut(s) 574
PctI GAATGC 1 cut(s) 941
PfeI GAWTC 7 cut(s) 150, 377, 787, 810, 1012, 1625, 2234
PfoI TCCNGGA 1 cut(s) 550
PkrI GCNGC 3 cut(s) 1028, 1273, 1535
PleI GAGTC 3 cut(s) 744, 1430, 1868
PpsI GAGTC 3 cut(s) 744, 1430, 1868
Ppu21I YACGTR 1 cut(s) 781
Psp1406I AACGTT 1 cut(s) 1367
Psp6I CCWGG 5 cut(s) 550, 1327, 1788, 1962, 2374
PspEI GGTNACC 2 cut(s) 274, 1208
PspFI CCCAGC 1 cut(s) 1543
PspGI CCWGG 5 cut(s) 550, 1327, 1788, 1962, 2374
PspN4I GGNNCC 3 cut(s) 17, 1981, 2192
PspPI GGNCC 2 cut(s) 762, 1562
PspXI VCTCGAGB 1 cut(s) 1848
PsrI GAACNNNNNNTAC 2 cut(s) 2382, 2414
PstI CTGCAG 3 cut(s) 130, 352, 1351
PsuI RGATCY 4 cut(s) 541, 732, 1900, 2190
RsaI GTAC 8 cut(s) 197, 515, 746, 783, 1052, 1150, 1579, 2298
RsaNI GTAC 8 cut(s) 196, 514, 745, 782, 1051, 1149, 1578, 2297
RseI CAYNNNNRTG 2 cut(s) 818, 934
SapI GCTCTTC 1 cut(s) 1789
SaqAI TTAA 6 cut(s) 50, 1062, 1370, 1739, 1883, 2160
SatI GCNGC 3 cut(s) 1027, 1272, 1534
Sau3AI GATC 9 cut(s) 541, 574, 732, 865, 1900, 1984, 2149, 2190, 2407
Sau96I GGNCC 2 cut(s) 762, 1562
SchI GAGTC 3 cut(s) 744, 1431, 1868
ScrFI CCNGG 7 cut(s) 162, 552, 1239, 1329, 1790, 1964, 2376
SduI GDGCHC 4 cut(s) 536, 825, 1502, 2243
SexAI ACCWGGT 1 cut(s) 2374
SfaNI GCATC 2 cut(s) 176, 2152
SfcI CTRYAG 4 cut(s) 126, 348, 627, 1347
Sfr274I CTCGAG 1 cut(s) 1848
SfuI TTCGAA 1 cut(s) 1006
SinI GGWCC 2 cut(s) 762, 1562
SlaI CTCGAG 1 cut(s) 1848
SmiMI CAYNNNNRTG 2 cut(s) 818, 934
SmlI CTYRAG 6 cut(s) 10, 49, 772, 1109, 1719, 1848
SmoI CTYRAG 6 cut(s) 10, 49, 772, 1109, 1719, 1848
SnaBI TACGTA 1 cut(s) 781
SpeI ACTAGT 1 cut(s) 902
SsiI CCGC 2 cut(s) 632, 1687
SspI AATATT 3 cut(s) 1918, 1974, 2200
SspMI CTAG 7 cut(s) 74, 119, 293, 903, 1878, 1911, 2105
StyD4I CCNGG 7 cut(s) 160, 550, 1237, 1327, 1788, 1962, 2374
StyI CCWWGG 1 cut(s) 1453
TaaI ACNGT 1 cut(s) 2262
TaiI ACGT 4 cut(s) 519, 770, 783, 1370
TaqI TCGA 4 cut(s) 1006, 1809, 1849, 2066
TatI WGTACW 2 cut(s) 744, 1050
TfiI GAWTC 7 cut(s) 150, 377, 787, 810, 1012, 1625, 2234
Tru1I TTAA 6 cut(s) 50, 1062, 1370, 1739, 1883, 2160
Tru9I TTAA 6 cut(s) 50, 1062, 1370, 1739, 1883, 2160
TscAI CASTG 6 cut(s) 366, 875, 1060, 1222, 2211, 2350
TseFI GTSAC 3 cut(s) 274, 363, 455
TseI GCWGC 3 cut(s) 1026, 1271, 1533
Tsp45I GTSAC 3 cut(s) 274, 363, 455
TspGWI ACGGA 3 cut(s) 583, 1279, 2400
TspRI CASTG 6 cut(s) 366, 875, 1060, 1222, 2211, 2350
Vha464I CTTAAG 1 cut(s) 49
VneI GTGCAC 2 cut(s) 821, 2239
VpaK11BI GGWCC 2 cut(s) 762, 1562
XagI CCTNNNNNAGG 1 cut(s) 549
XapI RAATTY 9 cut(s) 54, 334, 425, 710, 976, 1162, 1332, 1748, 2058
XbaI TCTAGA 1 cut(s) 1877
XceI RCATGY 2 cut(s) 2177, 2320
XcmI CCANNNNNNNNNTGG 2 cut(s) 1765, 1835
XhoI CTCGAG 1 cut(s) 1848
XspI CTAG 7 cut(s) 74, 119, 293, 903, 1878, 1911, 2105
Zsp2I ATGCAT 1 cut(s) 2179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.