Rh5DG188700

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000207
Physical Location & Seq
Reverse (-)
20929736 .. 20931207
1472 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG188700.1

Sequence Viewer

Length: 816 bp
ATGAAGGGAGCTAAGATACAGACTAGAGCAGGGACATGGAACGGCGCTGGATTAACTGGATATCAAGGAAAGCCAAATCCAGTAGCAAATTTTGAATTTGTGTTGACTAAGAATGAAGTCTATTATGAGTACACACTCATCAACAGGTCAATGTTTGCAAGATATGTGTTGAACCCGTATGGCCTTGCACAGTGGTTCACCTGGACAGATTACTCACACAGTTGGGAACCTTTCGTGTCATCCCAAGCAGATCAGTGTGATAATTATGCCTTTTGTGGTGCTAATGCTAGATGTAATGTCAGTAATACTCCTGTATGTGCATGCTTGAAAGGGTTTGTACCAAAATCTCCGAAAGATTGGAACTCAACAAATTGGTCTGAGGGATGTGTTCGGAAGACTCCATTAGCTTGCAGCTCTGCAGATGGCTTCTCAAAGTACAGTAACTTTAAACTGCCAGACACATCTTCTTCCTGGTATGACAAGAGCATGGGCCTTGAGGAATGCCAGGGATTATGTTTGCGAAACTGTTCATGTACTGCATGTGCAAATTTAGATGTCAGGGAAGGTGGAAGTGGCTGCTTGCTTTGGTTTGGGAACCTCAACGACATTAGAGAATTCACCTCTGATTATCAAGACCTCTATATACGGCTGGCTGCTTCAGACCTAGATCCTATTGTGAAAAAGAGCAAGTCCAGCAAGAAAAAGCTAGCAGGAATCCTGATCGGCTCATCTGTACTTCTTGTGGGAATGCTAATAGTTGGGTTTGTTTTGTATAAACGGAAGAGGAAACTCAGAAAGCAAGGTGAATTTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

271

Amino Acids

30.28

Weight (kDa)

8.96

Isoelectric Point (pI)

30.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S_locus_glycop PF00954 9 - 116 2.1e-24 S-locus glycoprotein domain
PAN_2 PF08276 137 - 203 6.5e-20 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27300
fragaria_vesca FvH4_3g15930 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g21350 FvH4_3g21400 FvH4_3g21400 FvH4_6g12332
malus_domestica MD02G1167100.v1.1 MD02G1167200.v1.1 MD03G1185600.v1.1 MD05G1213900.v1.1 MD05G1214100.v1.1 MD05G1214200.v1.1 MD05G1214700.v1.1 MD05G1216300.v1.1 MD05G1216800.v1.1 MD05G1217300.v1.1 MD05G1218000.v1.1 MD11G1231100.v1.1 MD11G1231200.v1.1 MD11G1231400.v1.1 MD11G1231500.v1.1 MD11G1232400.v1.1 MD11G1232500.v1.1
prunus_persica Prupe.4G142300_v2.0.a1 Prupe.4G142400_v2.0.a1 Prupe.4G142500_v2.0.a1 Prupe.4G142600_v2.0.a1 Prupe.4G142800_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195100_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195500_v2.0.a1 Prupe.4G195700_v2.0.a1 Prupe.4G195900_v2.0.a1
pyrus_communis pycom03g16540 pycom05g19920 pycom11g20460 pycom11g20480 pycom11g20490 pycom11g20500 pycom11g20530
rosa_chinensis RchiOBHm_Chr2g0139311 RchiOBHm_Chr2g0139351 RchiOBHm_Chr5g0026801 RchiOBHm_Chr5g0026851 RchiOBHm_Chr5g0026891 RchiOBHm_Chr5g0026911 RchiOBHm_Chr5g0036571 RchiOBHm_Chr5g0036591 RchiOBHm_Chr5g0036721 RchiOBHm_Chr5g0036741 RchiOBHm_Chr7g0222981
rosa_laevigata RLG00000002006 RLG00000019807 RLG00000032964 RLG00000032968 RLG00000032969 RLG00000033706 RLG00000033708 RLG00000033712 RLG00000033716
rosa_multiflora Rmu_co8256651.1_g000001 Rmu_co8279377.1_g000001 Rmu_co8429919.1_g000001 Rmu_co8490103.1_g000001 Rmu_sc0000774.1_g000002 Rmu_sc0002525.1_g000012 Rmu_sc0004223.1_g000008 Rmu_sc0004390.1_g000002 Rmu_sc0006059.1_g000014 Rmu_sc0006059.1_g000024 Rmu_sc0006514.1_g000020 Rmu_sc0014333.1_g000002 Rmu_ssc0000172.1_g000018 Rmu_ssc0000172.1_g000029
rosa_roxburghii Rroxscaffold_1G00044230 Rroxscaffold_1G00044260 Rroxscaffold_1G00044320 Rroxscaffold_1G00044340 Rroxscaffold_1G00052560 Rroxscaffold_1G00052570 Rroxscaffold_1G00052660 Rroxscaffold_1G00052720 Rroxscaffold_2G00105450 Rroxscaffold_2G00105460
rosa_rugosa Rorug02G0356900 Rorug02G0357000 Rorug02G0357100 Rorug02G0357200 Rorug02G0357300 Rorug02G0357400 Rorug02G0357500 Rorug02G0357600 Rorug05G0095400 Rorug05G0095500 Rorug05G0095500 Rorug05G0095600 Rorug05G0095700 Rorug05G0095800 Rorug05G0157700 Rorug05G0157800
rosa_samantha Rh2AG406800 Rh2AG406900 Rh2BG417300 Rh2CG392900 Rh2DG426200 Rh5AG189300 Rh5AG249400 Rh5AG250100 Rh5BG186300 Rh5BG186900 Rh5BG187000 Rh5BG249500 Rh5BG250600 Rh5BG250900 Rh5BG251500 Rh5BG251800 Rh5CG205500 Rh5CG206300 Rh5CG206700 Rh5CG283000 Rh5CG283300 Rh5CG283500 Rh5DG188500 Rh5DG188700 Rh5DG258700 Rh5DG258900 Rh5DG259600 Rh5DG259900 Rh7CG375800 Rh7DG352500
rosa_wichuraiana Rw2G032960 Rw2G032970 Rw2G033220 Rw2G033230 Rw5G017130 Rw5G017230 Rw5G022940 Rw5G022960 Rw5G022980 Rw7G030420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 662
AcsI RAATTY 5 cut(s) 88, 95, 547, 614, 806
AcuI CTGAAG 1 cut(s) 642
AfaI GTAC 5 cut(s) 131, 339, 437, 535, 735
AgsI TTSAA 3 cut(s) 95, 172, 328
AjnI CCWGG 3 cut(s) 200, 470, 504
AluBI AGCT 4 cut(s) 11, 407, 414, 706
AluI AGCT 4 cut(s) 11, 407, 414, 706
AlwI GGATC 1 cut(s) 662
AoxI GGCC 2 cut(s) 181, 490
ApeKI GCWGC 3 cut(s) 411, 576, 653
ApoI RAATTY 5 cut(s) 88, 95, 547, 614, 806
Asp700I GAANNNNTTC 1 cut(s) 526
AspLEI GCGC 1 cut(s) 47
AspS9I GGNCC 1 cut(s) 490
AsuHPI GGTGA 3 cut(s) 190, 610, 815
AsuNHI GCTAGC 1 cut(s) 706
BbsI GAAGAC 1 cut(s) 401
BbvI GCAGC 3 cut(s) 423, 563, 640
BccI CCATC 1 cut(s) 416
BceAI ACGGC 2 cut(s) 58, 662
BciT130I CCWGG 3 cut(s) 202, 472, 506
BfaI CTAG 4 cut(s) 24, 288, 665, 707
BfmI CTRYAG 1 cut(s) 417
BfoI RGCGCY 1 cut(s) 48
BisI GCNGC 3 cut(s) 412, 577, 654
BlsI GCNGC 3 cut(s) 413, 578, 655
Bme1390I CCNGG 3 cut(s) 202, 472, 506
BmgT120I GGNCC 1 cut(s) 490
BmiI GGNNCC 2 cut(s) 228, 596
BmrFI CCNGG 3 cut(s) 202, 472, 506
BmtI GCTAGC 1 cut(s) 710
BpiI GAAGAC 1 cut(s) 401
BpuEI CTTGAG 1 cut(s) 515
BsaJI CCNNGG 1 cut(s) 505
Bse1I ACTGG 2 cut(s) 61, 80
BseBI CCWGG 3 cut(s) 202, 472, 506
BseDI CCNNGG 1 cut(s) 505
BseGI GGATG 2 cut(s) 239, 389
BseMII CTCAG 2 cut(s) 369, 805
BseNI ACTGG 2 cut(s) 61, 80
BseXI GCAGC 3 cut(s) 423, 563, 640
BshFI GGCC 2 cut(s) 183, 492
BslFI GGGAC 1 cut(s) 46
BsmFI GGGAC 1 cut(s) 46
BsmI GAATGC 2 cut(s) 506, 753
BsnI GGCC 2 cut(s) 183, 492
Bsp143I GATC 3 cut(s) 250, 667, 720
BspANI GGCC 2 cut(s) 183, 492
BspCNI CTCAG 2 cut(s) 370, 804
BspLI GGNNCC 2 cut(s) 228, 596
BspMAI CTGCAG 1 cut(s) 421
BspOI GCTAGC 1 cut(s) 710
BspPI GGATC 1 cut(s) 662
BsrI ACTGG 2 cut(s) 61, 80
BssECI CCNNGG 1 cut(s) 505
BssMI GATC 3 cut(s) 250, 667, 720
Bst2UI CCWGG 3 cut(s) 202, 472, 506
Bst4CI ACNGT 4 cut(s) 192, 221, 440, 527
Bst6I CTCTTC 1 cut(s) 776
BstC8I GCNNGC 5 cut(s) 322, 409, 581, 651, 708
BstDEI CTNAG 4 cut(s) 12, 108, 378, 791
BstF5I GGATG 2 cut(s) 239, 389
BstH2I RGCGCY 1 cut(s) 48
BstHHI GCGC 1 cut(s) 47
BstKTI GATC 3 cut(s) 253, 670, 723
BstMBI GATC 3 cut(s) 250, 667, 720
BstMWI GCNNNNNNNGC 1 cut(s) 693
BstNI CCWGG 3 cut(s) 202, 472, 506
BstNSI RCATGY 2 cut(s) 324, 543
BstSCI CCNGG 3 cut(s) 200, 470, 504
BstSFI CTRYAG 1 cut(s) 417
BstV1I GCAGC 3 cut(s) 423, 563, 640
BstV2I GAAGAC 1 cut(s) 401
BstX2I RGATCY 1 cut(s) 667
BstYI RGATCY 1 cut(s) 667
BsuRI GGCC 2 cut(s) 183, 492
BtsCI GGATG 2 cut(s) 239, 389
BtsIMutI CAGTG 2 cut(s) 197, 260
Cac8I GCNNGC 5 cut(s) 322, 409, 581, 651, 708
CfoI GCGC 1 cut(s) 47
Cfr13I GGNCC 1 cut(s) 490
Csp6I GTAC 5 cut(s) 130, 338, 436, 534, 734
CviAII CATG 5 cut(s) 36, 321, 487, 531, 540
CviQI GTAC 5 cut(s) 130, 338, 436, 534, 734
DdeI CTNAG 4 cut(s) 12, 108, 378, 791
DpnI GATC 3 cut(s) 252, 669, 722
DpnII GATC 3 cut(s) 250, 667, 720
DraI TTTAAA 1 cut(s) 448
Eam1104I CTCTTC 1 cut(s) 776
EarI CTCTTC 1 cut(s) 776
Eco32I GATATC 1 cut(s) 62
Eco57I CTGAAG 1 cut(s) 642
EcoRI GAATTC 1 cut(s) 614
EcoRII CCWGG 3 cut(s) 200, 470, 504
EcoRV GATATC 1 cut(s) 62
FaeI CATG 5 cut(s) 39, 324, 490, 534, 543
FaqI GGGAC 1 cut(s) 46
FatI CATG 5 cut(s) 35, 320, 486, 530, 539
Fnu4HI GCNGC 3 cut(s) 412, 577, 654
FokI GGATG 2 cut(s) 226, 396
Fsp4HI GCNGC 3 cut(s) 412, 577, 654
FspBI CTAG 4 cut(s) 24, 288, 665, 707
GlaI GCGC 1 cut(s) 46
GluI GCNGC 3 cut(s) 412, 577, 654
HaeII RGCGCY 1 cut(s) 48
HaeIII GGCC 2 cut(s) 183, 492
HhaI GCGC 1 cut(s) 47
Hin1II CATG 5 cut(s) 39, 324, 490, 534, 543
Hin6I GCGC 1 cut(s) 45
HinP1I GCGC 1 cut(s) 45
HincII GTYRAC 1 cut(s) 105
HindII GTYRAC 1 cut(s) 105
HinfI GANTC 2 cut(s) 397, 714
HphI GGTGA 3 cut(s) 190, 610, 815
Hpy166II GTNNAC 3 cut(s) 105, 132, 198
Hpy188I TCNGA 7 cut(s) 351, 379, 393, 625, 661, 794, 815
Hpy188III TCNNGA 2 cut(s) 632, 718
Hpy8I GTNNAC 3 cut(s) 105, 132, 198
HpyAV CCTTC 1 cut(s) 557
HpyCH4III ACNGT 4 cut(s) 192, 221, 440, 527
HpyCH4V TGCA 7 cut(s) 158, 188, 320, 411, 419, 539, 545
HpyF10VI GCNNNNNNNGC 1 cut(s) 693
HpyF3I CTNAG 4 cut(s) 12, 108, 378, 791
Hsp92II CATG 5 cut(s) 39, 324, 490, 534, 543
HspAI GCGC 1 cut(s) 45
Kzo9I GATC 3 cut(s) 250, 667, 720
LmnI GCTCC 1 cut(s) 8
Lsp1109I GCAGC 3 cut(s) 423, 563, 640
MaeI CTAG 4 cut(s) 24, 288, 665, 707
MaeIII GTNAC 1 cut(s) 440
MalI GATC 3 cut(s) 252, 669, 722
MboI GATC 3 cut(s) 250, 667, 720
MboII GAAGA 4 cut(s) 406, 456, 459, 793
MflI RGATCY 1 cut(s) 667
MluCI AATT 7 cut(s) 88, 95, 262, 370, 547, 614, 806
MlyI GAGTC 1 cut(s) 391
MnlI CCTC 6 cut(s) 373, 490, 608, 631, 647, 777
MroXI GAANNNNTTC 1 cut(s) 526
MseI TTAA 2 cut(s) 53, 447
MspR9I CCNGG 3 cut(s) 202, 472, 506
Mva1269I GAATGC 2 cut(s) 506, 753
MvaI CCWGG 3 cut(s) 202, 472, 506
MwoI GCNNNNNNNGC 1 cut(s) 693
NdeII GATC 3 cut(s) 250, 667, 720
NheI GCTAGC 1 cut(s) 706
NlaIII CATG 5 cut(s) 39, 324, 490, 534, 543
NlaIV GGNNCC 2 cut(s) 228, 596
NspI RCATGY 2 cut(s) 324, 543
PaeI GCATGC 1 cut(s) 324
PctI GAATGC 2 cut(s) 506, 753
PdmI GAANNNNTTC 1 cut(s) 526
PfeI GAWTC 1 cut(s) 714
PkrI GCNGC 3 cut(s) 413, 578, 655
PleI GAGTC 1 cut(s) 391
PpsI GAGTC 1 cut(s) 391
Psp6I CCWGG 3 cut(s) 200, 470, 504
PspGI CCWGG 3 cut(s) 200, 470, 504
PspN4I GGNNCC 2 cut(s) 228, 596
PspPI GGNCC 1 cut(s) 490
PstI CTGCAG 1 cut(s) 421
PsuI RGATCY 1 cut(s) 667
RsaI GTAC 5 cut(s) 131, 339, 437, 535, 735
RsaNI GTAC 5 cut(s) 130, 338, 436, 534, 734
SaqAI TTAA 2 cut(s) 53, 447
SatI GCNGC 3 cut(s) 412, 577, 654
Sau3AI GATC 3 cut(s) 250, 667, 720
Sau96I GGNCC 1 cut(s) 490
SchI GAGTC 1 cut(s) 391
ScrFI CCNGG 3 cut(s) 202, 472, 506
SfcI CTRYAG 1 cut(s) 417
SmlI CTYRAG 1 cut(s) 494
SmoI CTYRAG 1 cut(s) 494
SphI GCATGC 1 cut(s) 324
Sse9I AATT 7 cut(s) 88, 95, 262, 370, 547, 614, 806
SspMI CTAG 4 cut(s) 24, 288, 665, 707
StyD4I CCNGG 3 cut(s) 200, 470, 504
TaaI ACNGT 4 cut(s) 192, 221, 440, 527
TasI AATT 7 cut(s) 88, 95, 262, 370, 547, 614, 806
TatI WGTACW 4 cut(s) 129, 435, 533, 733
TfiI GAWTC 1 cut(s) 714
Tru1I TTAA 2 cut(s) 53, 447
Tru9I TTAA 2 cut(s) 53, 447
TscAI CASTG 2 cut(s) 197, 260
TseI GCWGC 3 cut(s) 411, 576, 653
TspDTI ATGAA 3 cut(s) 17, 129, 519
TspGWI ACGGA 1 cut(s) 793
TspRI CASTG 2 cut(s) 197, 260
XapI RAATTY 5 cut(s) 88, 95, 547, 614, 806
XceI RCATGY 2 cut(s) 324, 543
XmnI GAANNNNTTC 1 cut(s) 526
XspI CTAG 4 cut(s) 24, 288, 665, 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.