Rroxscaffold_1G00044230

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000207
Physical Location & Seq
Reverse (-)
63002979 .. 63006243
3265 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00044230.1

Sequence Viewer

Length: 2262 bp
ATGAAGAACCTCTGCTTCATTCTTCTCTGCATCTGTTTCAGCAAACTTTCATCTGCAGCTGATACCCTTTATTCATCTCAGAACATCACTGATGGCAACTCCTTGGTTTCCCCAGGTGGAGTATTTCAACTGGGTTTCTTCAGCCCAGGCAGTTCCAAATCCCGCTACTTGGGGATTTGGTACAAGAACATCTCAGTCCAAACCGTCGTTTGGGTTGCGAATCGAGGCACCCCGATCAACGACTCCTCAGGCCATTTGATGATCAACATCACCACAGGGGCTCTAGTTCTTCTCAACAACAACAAGACAGTTTTTTGGTCGTCAAATTCGACGAAACAAACCCAGAATATTAGTCCTGTCGTTCAGTTATTAGACAATGGCAATCTTATTCTCAGAGACGAGAAAGAAGGAGCAAACTCCGAAAGCTATCTGTGGCAAAGCTTCGACTATCCGTCGGATACTTTGTTACCGGGGATGAAGCTGGGATGGGACTTGAGAACCGGGCTGAACCGGAGACTGACGGCGTGGAAGAGTCCAGATGACCCTTCTCCTGGAGACTTCACATGGGAGATGCAGCTGCATAACTATCCTGAGCCAGCTATGTTCAGAGCGGGCAAAGAGTTTTTGAGGAGTGGTCCGTGGAATGGTGTTTTATTCAGTGGAAAACCGGCAAAGGCGCTACCGGCGTTAAACTTCAGCTTCTTCATGGATGAGAATGAGGTTTACACAACAATTGGAATGGTGGACAAGTCAGCATTAGGGAGGATGATGCTGAATTTAACTGCAGATTACTACCGCCAGTCGTGGATTTGGTCTGAAGCAGACCAGAATTGGACACTCTATGCAGCATTTCCTAGGGATCCTTGTGACAGCTATGCCAAGTGTGGAGGAAATGGAAACTGTATGCTTAGTGCATCCCCGATGTGTCAATGTTTAGACAGGTTCAGGCCTAGATCAGTGGAGAAGTGGAGCTTGAACAATTTTTCGCTCGGGTGTGAGCGAAAGACGCCATTGAGGTGCAAGAATGATGGATTTGTGACTTATGCTGGGTTAAAATTGCCCGATACAACGCATACTTGGATCGATAAAGCTATGAATCTCAAGGAGTGCAGGGCCAAATGCTTGTCTAATTGTTCTTGTTCCGCGTACACTAACTTGGATGTCAGAGGAGGAGGTAGTGGTTGTGCAATCTGGTTTGGTGATCTTGTAGACTTGAAGCAGATTCCGGGTGGTGAACAGGATATTTACATTAAGATCTCTGCTGCAGAACTAGGAGGGAAAGATAAGAAGTGGAAGATAGGAGTGATAGTTGCATCTGCCGTTGCTGTAATTCTTGCGATGTTCTTATTTGGCTATTGTTACATTCTCAGACTCAGACACAGGAAAAGTTTCAAAGGTACTCAAAACAACATTGAAGAGCAGAAGGATGAAGACCTGGAGCTGCCTTTGTTCGACTTGTCAACAATAGAGACTGCTACCAATAAATTCTCAATCAATAATAAACTTGGAGAAGGTGGTTTTGGACCTGTGTACAAGGGTACACTAATAGATGGAAAAGAAATTGCAGTGAAGCGGCTCTCAAGAAGTTCTGGACAAGGAATGAAAGAGTTCAAAAATGAAGTAATACTGATTGCAAAACTTCAGCACCGCAATCTGGACTCCAGGCTGAGGATTATACACAGAGATCTAAAAGCAAGCAATGTCTTGCTTGATCGTGAGATGAACCCCAAAATCTCAGATTTTGGTCTTGCTAGAACTTTTGGTGGTGACCAAACTGAAGGAAACACAAACAGAGTAGTTGGAACATATGGTTACATGGCACCTGAATATGCTATTGAAGGGCAGTTCTCAGTAAAATCCGATGTCTTTAGCTTTGGTATTTTAATGCTGGAGCTAATGAGTGGAAAGAAAAGTAAAGGGTTCTATCACTTGAACCACGACCTTAATCTTATTGGAAATGCATGGAGGTTATGGAACGGAGGGCGGCCATTAGAACTGATAGATGAAGGGTTGGGGGAGTCCTGCAACTTAACGGAGGTGTTGCGATGCATCCATGTCAGTCTCTTGTGTGTACAACAACAGCCTGACGACAGGCCGACCATGTCATCTGTAGTTCAGATGTTATGTAGTGGAAGTGCCTTGCCTCAGCCTAAAGAACCAGGATTCTTTTCAGAGAAAGATTTGGTTGGAGGAGGCTTTCCCTTTCTTAACAACGAAGCGTCTTCTAATGGATTAACCATTTCCCTACTCGAGGCTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

753

Amino Acids

83.73

Weight (kDa)

8.11

Isoelectric Point (pI)

40.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 68 - 177 2.5e-37 D-mannose binding lectin
S_locus_glycop PF00954 210 - 318 1.8e-21 S-locus glycoprotein domain
PAN_2 PF08276 340 - 405 1.3e-22 PAN-like domain
PK_Tyr_Ser-Thr PF07714 557 - 708 3.2e-23 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 557 - 639 2.7e-22 Protein kinase domain
DUF3403 PF11883 712 - 753 8.5e-06 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27300
fragaria_vesca FvH4_3g15930 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g21350 FvH4_3g21400 FvH4_3g21400 FvH4_6g12332
malus_domestica MD02G1167100.v1.1 MD02G1167200.v1.1 MD03G1185600.v1.1 MD05G1213900.v1.1 MD05G1214100.v1.1 MD05G1214200.v1.1 MD05G1214700.v1.1 MD05G1216300.v1.1 MD05G1216800.v1.1 MD05G1217300.v1.1 MD05G1218000.v1.1 MD11G1231100.v1.1 MD11G1231200.v1.1 MD11G1231400.v1.1 MD11G1231500.v1.1 MD11G1232400.v1.1 MD11G1232500.v1.1
prunus_persica Prupe.4G142300_v2.0.a1 Prupe.4G142400_v2.0.a1 Prupe.4G142500_v2.0.a1 Prupe.4G142600_v2.0.a1 Prupe.4G142800_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195100_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195500_v2.0.a1 Prupe.4G195700_v2.0.a1 Prupe.4G195900_v2.0.a1
pyrus_communis pycom03g16540 pycom05g19920 pycom11g20460 pycom11g20480 pycom11g20490 pycom11g20500 pycom11g20530
rosa_chinensis RchiOBHm_Chr2g0139311 RchiOBHm_Chr2g0139351 RchiOBHm_Chr5g0026801 RchiOBHm_Chr5g0026851 RchiOBHm_Chr5g0026891 RchiOBHm_Chr5g0026911 RchiOBHm_Chr5g0036571 RchiOBHm_Chr5g0036591 RchiOBHm_Chr5g0036721 RchiOBHm_Chr5g0036741 RchiOBHm_Chr7g0222981
rosa_laevigata RLG00000002006 RLG00000019807 RLG00000032964 RLG00000032968 RLG00000032969 RLG00000033706 RLG00000033708 RLG00000033712 RLG00000033716
rosa_multiflora Rmu_co8256651.1_g000001 Rmu_co8279377.1_g000001 Rmu_co8429919.1_g000001 Rmu_co8490103.1_g000001 Rmu_sc0000774.1_g000002 Rmu_sc0002525.1_g000012 Rmu_sc0004223.1_g000008 Rmu_sc0004390.1_g000002 Rmu_sc0006059.1_g000014 Rmu_sc0006059.1_g000024 Rmu_sc0006514.1_g000020 Rmu_sc0014333.1_g000002 Rmu_ssc0000172.1_g000018 Rmu_ssc0000172.1_g000029
rosa_roxburghii Rroxscaffold_1G00044230 Rroxscaffold_1G00044260 Rroxscaffold_1G00044320 Rroxscaffold_1G00044340 Rroxscaffold_1G00052560 Rroxscaffold_1G00052570 Rroxscaffold_1G00052660 Rroxscaffold_1G00052720 Rroxscaffold_2G00105450 Rroxscaffold_2G00105460
rosa_rugosa Rorug02G0356900 Rorug02G0357000 Rorug02G0357100 Rorug02G0357200 Rorug02G0357300 Rorug02G0357400 Rorug02G0357500 Rorug02G0357600 Rorug05G0095400 Rorug05G0095500 Rorug05G0095500 Rorug05G0095600 Rorug05G0095700 Rorug05G0095800 Rorug05G0157700 Rorug05G0157800
rosa_samantha Rh2AG406800 Rh2AG406900 Rh2BG417300 Rh2CG392900 Rh2DG426200 Rh5AG189300 Rh5AG249400 Rh5AG250100 Rh5BG186300 Rh5BG186900 Rh5BG187000 Rh5BG249500 Rh5BG250600 Rh5BG250900 Rh5BG251500 Rh5BG251800 Rh5CG205500 Rh5CG206300 Rh5CG206700 Rh5CG283000 Rh5CG283300 Rh5CG283500 Rh5DG188500 Rh5DG188700 Rh5DG258700 Rh5DG258900 Rh5DG259600 Rh5DG259900 Rh7CG375800 Rh7DG352500
rosa_wichuraiana Rw2G032960 Rw2G032970 Rw2G033220 Rw2G033230 Rw5G017130 Rw5G017230 Rw5G022940 Rw5G022960 Rw5G022980 Rw7G030420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 227, 1819
AccBSI CCGCTC 1 cut(s) 611
AccI GTMKAC 1 cut(s) 1209
AccII CGCG 1 cut(s) 1145
AciI CCGC 7 cut(s) 163, 611, 796, 1143, 1573, 1648, 1984
AclWI GGATC 3 cut(s) 854, 867, 1088
AcoI YGGCCR 1 cut(s) 1985
AcsI RAATTY 3 cut(s) 325, 775, 1484
AcuI CTGAAG 5 cut(s) 124, 679, 837, 1625, 1797
AcyI GRCGYC 1 cut(s) 1007
AfaI GTAC 6 cut(s) 182, 1148, 1399, 1532, 1540, 2073
AfiI CCNNNNNNNGG 7 cut(s) 169, 210, 551, 644, 1654, 1668, 2251
AgsI TTSAA 8 cut(s) 128, 976, 1216, 1393, 1415, 1612, 1838, 1933
AhdI GACNNNNNGTC 1 cut(s) 451
AjnI CCWGG 6 cut(s) 112, 145, 550, 1434, 1661, 2158
AjuI GAANNNNNNNTTGG 2 cut(s) 1503, 1535
Alw26I GTCTC 5 cut(s) 390, 508, 549, 1463, 2066
AlwI GGATC 3 cut(s) 854, 867, 1088
Ama87I CYCGRG 2 cut(s) 989, 2249
AoxI GGCC 5 cut(s) 250, 947, 1113, 1985, 2093
ApeKI GCWGC 6 cut(s) 56, 574, 577, 845, 1262, 1441
ApoI RAATTY 3 cut(s) 325, 775, 1484
Asp700I GAANNNNTTC 2 cut(s) 1388, 1607
AspA2I CCTAGG 1 cut(s) 854
AspLEI GCGC 1 cut(s) 679
AspS9I GGNCC 3 cut(s) 635, 1113, 1523
AsuC2I CCSGG 3 cut(s) 471, 502, 1227
AsuHPI GGTGA 4 cut(s) 262, 1211, 1244, 1778
AvaI CYCGRG 2 cut(s) 989, 2249
AvaII GGWCC 2 cut(s) 635, 1523
AvrII CCTAGG 1 cut(s) 854
AxyI CCTNAGG 1 cut(s) 247
BamHI GGATCC 1 cut(s) 859
BanI GGYRCC 2 cut(s) 227, 1819
BanII GRGCYC 1 cut(s) 283
BbsI GAAGAC 2 cut(s) 1437, 2214
BbvCI CCTCAGC 2 cut(s) 1667, 2145
BbvI GCAGC 6 cut(s) 68, 564, 586, 857, 1249, 1428
BccI CCATC 4 cut(s) 86, 480, 1022, 1544
BceAI ACGGC 2 cut(s) 537, 1304
BciT130I CCWGG 6 cut(s) 114, 147, 552, 1436, 1663, 2160
BciVI GTATCC 1 cut(s) 451
BclI TGATCA 1 cut(s) 261
BcnI CCSGG 3 cut(s) 471, 502, 1227
BcoDI GTCTC 5 cut(s) 390, 508, 549, 1463, 2066
BfaI CTAG 5 cut(s) 284, 855, 951, 1271, 1752
BfmI CTRYAG 4 cut(s) 54, 783, 1263, 2109
BfoI RGCGCY 1 cut(s) 680
BfuI GTATCC 1 cut(s) 451
BglII AGATCT 2 cut(s) 1254, 1684
BisI GCNGC 8 cut(s) 57, 575, 578, 846, 1263, 1442, 1574, 1985
BlnI CCTAGG 1 cut(s) 854
BlsI GCNGC 8 cut(s) 58, 576, 579, 847, 1264, 1443, 1575, 1986
Bme1390I CCNGG 9 cut(s) 114, 147, 471, 502, 552, 1227, 1436, 1663, 2160
Bme18I GGWCC 2 cut(s) 635, 1523
BmeRI GACNNNNNGTC 1 cut(s) 451
BmeT110I CYCGRG 2 cut(s) 989, 2249
BmgT120I GGNCC 3 cut(s) 635, 1113, 1523
BmiI GGNNCC 3 cut(s) 229, 861, 1821
BmrFI CCNGG 9 cut(s) 114, 147, 471, 502, 552, 1227, 1436, 1663, 2160
BmrI ACTGGG 1 cut(s) 140
BmsI GCATC 7 cut(s) 39, 561, 759, 923, 1322, 2036, 2058
BmuI ACTGGG 1 cut(s) 140
BpiI GAAGAC 2 cut(s) 1437, 2214
BpmI CTGGAG 4 cut(s) 573, 1457, 1645, 1910
Bpu10I CCTNAGC 3 cut(s) 591, 1667, 2145
BpuEI CTTGAG 3 cut(s) 514, 1085, 1564
BpuMI CCSGG 3 cut(s) 471, 502, 1227
Bsa29I ATCGAT 1 cut(s) 1083
BsaBI GATNNNNATC 1 cut(s) 266
BsaHI GRCGYC 1 cut(s) 1007
BsaJI CCNNGG 6 cut(s) 102, 112, 145, 470, 638, 854
BsaWI WCCGGW 1 cut(s) 510
BsaXI ACNNNNNCTCC 2 cut(s) 1293, 1323
Bsc4I CCNNNNNNNGG 7 cut(s) 169, 210, 551, 644, 1654, 1668, 2251
Bse118I RCCGGY 2 cut(s) 667, 682
Bse1I ACTGG 2 cut(s) 135, 799
Bse21I CCTNAGG 1 cut(s) 247
Bse3DI GCAATG 1 cut(s) 1705
Bse8I GATNNNNATC 1 cut(s) 266
BseBI CCWGG 6 cut(s) 114, 147, 552, 1436, 1663, 2160
BseCI ATCGAT 1 cut(s) 1083
BseDI CCNNGG 6 cut(s) 102, 112, 145, 470, 638, 854
BseGI GGATG 8 cut(s) 480, 491, 715, 771, 914, 1165, 1432, 2049
BseJI GATNNNNATC 1 cut(s) 266
BseLI CCNNNNNNNGG 7 cut(s) 169, 210, 551, 644, 1654, 1668, 2251
BseMI GCAATG 1 cut(s) 1705
BseNI ACTGG 2 cut(s) 135, 799
BseRI GAGGAG 5 cut(s) 235, 643, 1182, 1185, 2205
BseXI GCAGC 6 cut(s) 68, 564, 586, 857, 1249, 1428
BseYI CCCAGC 2 cut(s) 481, 1046
BsgI GTGCAG 1 cut(s) 1129
Bsh1236I CGCG 1 cut(s) 1145
BshFI GGCC 5 cut(s) 252, 949, 1115, 1987, 2095
BshNI GGYRCC 2 cut(s) 227, 1819
BshVI ATCGAT 1 cut(s) 1083
BsiHKCI CYCGRG 2 cut(s) 989, 2249
BsiSI CCGG 6 cut(s) 470, 501, 511, 668, 683, 1226
BslFI GGGAC 1 cut(s) 503
BslI CCNNNNNNNGG 7 cut(s) 169, 210, 551, 644, 1654, 1668, 2251
BsmAI GTCTC 5 cut(s) 390, 508, 549, 1463, 2066
BsmBI CGTCTC 1 cut(s) 390
BsmFI GGGAC 1 cut(s) 503
BsnI GGCC 5 cut(s) 252, 949, 1115, 1987, 2095
BsoBI CYCGRG 2 cut(s) 989, 2249
Bsp1286I GDGCHC 1 cut(s) 283
Bsp1407I TGTACA 2 cut(s) 1530, 2071
Bsp143I GATC 9 cut(s) 234, 261, 859, 953, 1080, 1201, 1254, 1684, 1711
BspACI CCGC 7 cut(s) 163, 611, 796, 1143, 1573, 1648, 1984
BspANI GGCC 5 cut(s) 252, 949, 1115, 1987, 2095
BspDI ATCGAT 1 cut(s) 1083
BspFNI CGCG 1 cut(s) 1145
BspLI GGNNCC 3 cut(s) 229, 861, 1821
BspMAI CTGCAG 3 cut(s) 58, 787, 1267
BspPI GGATC 3 cut(s) 854, 867, 1088
BspQI GCTCTTC 1 cut(s) 1410
BspT107I GGYRCC 2 cut(s) 227, 1819
BsrBI CCGCTC 1 cut(s) 611
BsrDI GCAATG 1 cut(s) 1705
BsrFI RCCGGY 2 cut(s) 667, 682
BsrGI TGTACA 2 cut(s) 1530, 2071
BsrI ACTGG 2 cut(s) 135, 799
BssAI RCCGGY 2 cut(s) 667, 682
BssECI CCNNGG 6 cut(s) 102, 112, 145, 470, 638, 854
BssMI GATC 9 cut(s) 234, 261, 859, 953, 1080, 1201, 1254, 1684, 1711
BssNI GRCGYC 1 cut(s) 1007
BssT1I CCWWGG 2 cut(s) 102, 854
Bst2UI CCWGG 6 cut(s) 114, 147, 552, 1436, 1663, 2160
Bst4CI ACNGT 3 cut(s) 205, 310, 902
Bst6I CTCTTC 2 cut(s) 524, 1410
BstACI GRCGYC 1 cut(s) 1007
BstAUI TGTACA 2 cut(s) 1530, 2071
BstC8I GCNNGC 3 cut(s) 597, 613, 1696
BstDSI CCRYGG 1 cut(s) 638
BstEII GGTNACC 1 cut(s) 1766
BstENI CCTNNNNNAGG 1 cut(s) 2249
BstF5I GGATG 8 cut(s) 480, 491, 715, 771, 914, 1165, 1432, 2049
BstFNI CGCG 1 cut(s) 1145
BstH2I RGCGCY 1 cut(s) 680
BstHHI GCGC 1 cut(s) 679
BstKTI GATC 9 cut(s) 237, 264, 862, 956, 1083, 1204, 1257, 1687, 1714
BstMAI GTCTC 5 cut(s) 390, 508, 549, 1463, 2066
BstMBI GATC 9 cut(s) 234, 261, 859, 953, 1080, 1201, 1254, 1684, 1711
BstMWI GCNNNNNNNGC 2 cut(s) 683, 1006
BstNI CCWGG 6 cut(s) 114, 147, 552, 1436, 1663, 2160
BstPI GGTNACC 1 cut(s) 1766
BstSCI CCNGG 9 cut(s) 112, 145, 469, 500, 550, 1225, 1434, 1661, 2158
BstSFI CTRYAG 4 cut(s) 54, 783, 1263, 2109
BstUI CGCG 1 cut(s) 1145
BstV1I GCAGC 6 cut(s) 68, 564, 586, 857, 1249, 1428
BstV2I GAAGAC 2 cut(s) 1437, 2214
BstX2I RGATCY 3 cut(s) 859, 1254, 1684
BstYI RGATCY 3 cut(s) 859, 1254, 1684
Bsu15I ATCGAT 1 cut(s) 1083
Bsu36I CCTNAGG 1 cut(s) 247
BsuI GTATCC 1 cut(s) 451
BsuRI GGCC 5 cut(s) 252, 949, 1115, 1987, 2095
BsuTUI ATCGAT 1 cut(s) 1083
BtgI CCRYGG 1 cut(s) 638
BtgZI GCGATG 2 cut(s) 1352, 2059
BtsCI GGATG 8 cut(s) 480, 491, 715, 771, 914, 1165, 1432, 2049
BtsI GCAGTG 1 cut(s) 1572
BtsIMutI CAGTG 4 cut(s) 87, 664, 963, 1572
Cac8I GCNNGC 3 cut(s) 597, 613, 1696
CfoI GCGC 1 cut(s) 679
Cfr10I RCCGGY 2 cut(s) 667, 682
Cfr13I GGNCC 3 cut(s) 635, 1113, 1523
ClaI ATCGAT 1 cut(s) 1083
CseI GACGC 2 cut(s) 1015, 2208
Csp6I GTAC 6 cut(s) 181, 1147, 1398, 1531, 1539, 2072
CviAII CATG 6 cut(s) 564, 706, 1816, 1962, 2054, 2101
CviQI GTAC 6 cut(s) 181, 1147, 1398, 1531, 1539, 2072
DpnI GATC 9 cut(s) 236, 263, 861, 955, 1082, 1203, 1256, 1686, 1713
DpnII GATC 9 cut(s) 234, 261, 859, 953, 1080, 1201, 1254, 1684, 1711
DriI GACNNNNNGTC 1 cut(s) 451
EaeI YGGCCR 1 cut(s) 1985
Eam1104I CTCTTC 2 cut(s) 524, 1410
Eam1105I GACNNNNNGTC 1 cut(s) 451
EarI CTCTTC 2 cut(s) 524, 1410
Eco130I CCWWGG 2 cut(s) 102, 854
Eco147I AGGCCT 1 cut(s) 949
Eco24I GRGCYC 1 cut(s) 283
Eco47I GGWCC 2 cut(s) 635, 1523
Eco57I CTGAAG 5 cut(s) 124, 679, 837, 1625, 1797
Eco81I CCTNAGG 1 cut(s) 247
Eco88I CYCGRG 2 cut(s) 989, 2249
Eco91I GGTNACC 1 cut(s) 1766
EcoNI CCTNNNNNAGG 1 cut(s) 2249
EcoO65I GGTNACC 1 cut(s) 1766
EcoRII CCWGG 6 cut(s) 112, 145, 550, 1434, 1661, 2158
EcoT14I CCWWGG 2 cut(s) 102, 854
EcoT22I ATGCAT 2 cut(s) 1963, 2051
EcoT38I GRGCYC 1 cut(s) 283
ErhI CCWWGG 2 cut(s) 102, 854
Esp3I CGTCTC 1 cut(s) 390
FaeI CATG 6 cut(s) 567, 709, 1819, 1965, 2057, 2104
FalI AAGNNNNNCTT 2 cut(s) 956, 988
FaqI GGGAC 1 cut(s) 503
FatI CATG 6 cut(s) 563, 705, 1815, 1961, 2053, 2100
FauI CCCGC 2 cut(s) 170, 604
FauNDI CATATG 1 cut(s) 1807
FbaI TGATCA 1 cut(s) 261
FblI GTMKAC 1 cut(s) 1209
Fnu4HI GCNGC 8 cut(s) 57, 575, 578, 846, 1263, 1442, 1574, 1985
FokI GGATG 8 cut(s) 487, 498, 722, 778, 901, 1172, 1439, 2036
FriOI GRGCYC 1 cut(s) 283
Fsp4HI GCNGC 8 cut(s) 57, 575, 578, 846, 1263, 1442, 1574, 1985
FspBI CTAG 5 cut(s) 284, 855, 951, 1271, 1752
GlaI GCGC 1 cut(s) 678
GluI GCNGC 8 cut(s) 57, 575, 578, 846, 1263, 1442, 1574, 1985
GsaI CCCAGC 2 cut(s) 485, 1050
GsuI CTGGAG 4 cut(s) 573, 1457, 1645, 1910
HaeII RGCGCY 1 cut(s) 680
HaeIII GGCC 5 cut(s) 252, 949, 1115, 1987, 2095
HapII CCGG 6 cut(s) 470, 501, 511, 668, 683, 1226
HgaI GACGC 2 cut(s) 1015, 2208
HhaI GCGC 1 cut(s) 679
Hin1I GRCGYC 1 cut(s) 1007
Hin1II CATG 6 cut(s) 567, 709, 1819, 1965, 2057, 2104
Hin6I GCGC 1 cut(s) 677
HinP1I GCGC 1 cut(s) 677
HincII GTYRAC 1 cut(s) 1461
HindII GTYRAC 1 cut(s) 1461
HindIII AAGCTT 1 cut(s) 439
HinfI GANTC 9 cut(s) 220, 242, 532, 1096, 1222, 1371, 1658, 2018, 2163
HpaII CCGG 6 cut(s) 470, 501, 511, 668, 683, 1226
HphI GGTGA 4 cut(s) 262, 1211, 1244, 1778
Hpy166II GTNNAC 9 cut(s) 724, 745, 1149, 1210, 1235, 1461, 1531, 1541, 2072
Hpy188III TCNNGA 6 cut(s) 536, 590, 1581, 1590, 1655, 1715
Hpy8I GTNNAC 9 cut(s) 724, 745, 1149, 1210, 1235, 1461, 1531, 1541, 2072
Hpy99I CGWCG 3 cut(s) 209, 334, 457
HpyAV CCTTC 7 cut(s) 401, 555, 1417, 1505, 1772, 1832, 2000
HpyCH4III ACNGT 3 cut(s) 205, 310, 902
HpyF10VI GCNNNNNNNGC 2 cut(s) 683, 1006
Hsp92I GRCGYC 1 cut(s) 1007
Hsp92II CATG 6 cut(s) 567, 709, 1819, 1965, 2057, 2104
HspAI GCGC 1 cut(s) 677
Ksp22I TGATCA 1 cut(s) 261
Kzo9I GATC 9 cut(s) 234, 261, 859, 953, 1080, 1201, 1254, 1684, 1711
LguI GCTCTTC 1 cut(s) 1410
LmnI GCTCC 4 cut(s) 410, 969, 1438, 1891
Lsp1109I GCAGC 6 cut(s) 68, 564, 586, 857, 1249, 1428
LweI GCATC 7 cut(s) 39, 561, 759, 923, 1322, 2036, 2058
MaeI CTAG 5 cut(s) 284, 855, 951, 1271, 1752
MaeIII GTNAC 6 cut(s) 465, 866, 1036, 1358, 1766, 1811
MalI GATC 9 cut(s) 236, 263, 861, 955, 1082, 1203, 1256, 1686, 1713
MbiI CCGCTC 1 cut(s) 611
MboI GATC 9 cut(s) 234, 261, 859, 953, 1080, 1201, 1254, 1684, 1711
MfeI CAATTG 1 cut(s) 732
MflI RGATCY 3 cut(s) 859, 1254, 1684
MhlI GDGCHC 1 cut(s) 283
MlyI GAGTC 5 cut(s) 236, 541, 1365, 1652, 2027
MmeI TCCRAC 3 cut(s) 435, 1780, 2167
Mph1103I ATGCAT 2 cut(s) 1963, 2051
MroXI GAANNNNTTC 2 cut(s) 1388, 1607
MseI TTAA 9 cut(s) 689, 779, 1052, 1251, 1883, 1944, 2030, 2208, 2234
MslI CAYNNNNRTG 1 cut(s) 1015
MspA1I CMGCKG 2 cut(s) 59, 577
MspI CCGG 6 cut(s) 470, 501, 511, 668, 683, 1226
MspR9I CCNGG 9 cut(s) 114, 147, 471, 502, 552, 1227, 1436, 1663, 2160
MunI CAATTG 1 cut(s) 732
MvaI CCWGG 6 cut(s) 114, 147, 552, 1436, 1663, 2160
MvnI CGCG 1 cut(s) 1145
MwoI GCNNNNNNNGC 2 cut(s) 683, 1006
NciI CCSGG 3 cut(s) 471, 502, 1227
NdeI CATATG 1 cut(s) 1807
NdeII GATC 9 cut(s) 234, 261, 859, 953, 1080, 1201, 1254, 1684, 1711
NlaIII CATG 6 cut(s) 567, 709, 1819, 1965, 2057, 2104
NlaIV GGNNCC 3 cut(s) 229, 861, 1821
NmuCI GTSAC 3 cut(s) 866, 1036, 1766
NsiI ATGCAT 2 cut(s) 1963, 2051
PaeR7I CTCGAG 1 cut(s) 2249
PceI AGGCCT 1 cut(s) 949
PciSI GCTCTTC 1 cut(s) 1410
PcsI WCGNNNNNNNCGW 1 cut(s) 326
PdmI GAANNNNTTC 2 cut(s) 1388, 1607
PfeI GAWTC 4 cut(s) 220, 1096, 1222, 2163
PflFI GACNNNGTC 1 cut(s) 2101
PfoI TCCNGGA 1 cut(s) 550
PkrI GCNGC 8 cut(s) 58, 576, 579, 847, 1264, 1443, 1575, 1986
PleI GAGTC 5 cut(s) 236, 540, 1365, 1652, 2026
PpsI GAGTC 5 cut(s) 236, 540, 1365, 1652, 2026
Psp6I CCWGG 6 cut(s) 112, 145, 550, 1434, 1661, 2158
PspEI GGTNACC 1 cut(s) 1766
PspFI CCCAGC 2 cut(s) 481, 1046
PspGI CCWGG 6 cut(s) 112, 145, 550, 1434, 1661, 2158
PspN4I GGNNCC 3 cut(s) 229, 861, 1821
PspPI GGNCC 3 cut(s) 635, 1113, 1523
PspXI VCTCGAGB 1 cut(s) 2249
PstI CTGCAG 3 cut(s) 58, 787, 1267
PsuI RGATCY 3 cut(s) 859, 1254, 1684
PsyI GACNNNGTC 1 cut(s) 2101
PvuII CAGCTG 2 cut(s) 59, 577
RsaI GTAC 6 cut(s) 182, 1148, 1399, 1532, 1540, 2073
RsaNI GTAC 6 cut(s) 181, 1147, 1398, 1531, 1539, 2072
RseI CAYNNNNRTG 1 cut(s) 1015
SapI GCTCTTC 1 cut(s) 1410
SaqAI TTAA 9 cut(s) 689, 779, 1052, 1251, 1883, 1944, 2030, 2208, 2234
SatI GCNGC 8 cut(s) 57, 575, 578, 846, 1263, 1442, 1574, 1985
Sau3AI GATC 9 cut(s) 234, 261, 859, 953, 1080, 1201, 1254, 1684, 1711
Sau96I GGNCC 3 cut(s) 635, 1113, 1523
SchI GAGTC 5 cut(s) 236, 541, 1365, 1652, 2027
ScrFI CCNGG 9 cut(s) 114, 147, 471, 502, 552, 1227, 1436, 1663, 2160
SduI GDGCHC 1 cut(s) 283
SfaNI GCATC 7 cut(s) 39, 561, 759, 923, 1322, 2036, 2058
SfcI CTRYAG 4 cut(s) 54, 783, 1263, 2109
Sfr274I CTCGAG 1 cut(s) 2249
SinI GGWCC 2 cut(s) 635, 1523
SlaI CTCGAG 1 cut(s) 2249
SmiMI CAYNNNNRTG 1 cut(s) 1015
SmlI CTYRAG 4 cut(s) 493, 1100, 1579, 2249
SmoI CTYRAG 4 cut(s) 493, 1100, 1579, 2249
SseBI AGGCCT 1 cut(s) 949
SsiI CCGC 7 cut(s) 163, 611, 796, 1143, 1573, 1648, 1984
SspI AATATT 1 cut(s) 349
SspMI CTAG 5 cut(s) 284, 855, 951, 1271, 1752
StuI AGGCCT 1 cut(s) 949
StyD4I CCNGG 9 cut(s) 112, 145, 469, 500, 550, 1225, 1434, 1661, 2158
StyI CCWWGG 2 cut(s) 102, 854
TaaI ACNGT 3 cut(s) 205, 310, 902
TaqI TCGA 6 cut(s) 223, 329, 444, 1083, 1452, 2250
TatI WGTACW 2 cut(s) 1530, 2071
TauI GCSGC 2 cut(s) 1576, 1987
TfiI GAWTC 4 cut(s) 220, 1096, 1222, 2163
Tru1I TTAA 9 cut(s) 689, 779, 1052, 1251, 1883, 1944, 2030, 2208, 2234
Tru9I TTAA 9 cut(s) 689, 779, 1052, 1251, 1883, 1944, 2030, 2208, 2234
TscAI CASTG 4 cut(s) 94, 664, 963, 1572
TseFI GTSAC 3 cut(s) 866, 1036, 1766
TseI GCWGC 6 cut(s) 56, 574, 577, 845, 1262, 1441
Tsp45I GTSAC 3 cut(s) 866, 1036, 1766
TspGWI ACGGA 4 cut(s) 441, 627, 1992, 2048
TspRI CASTG 4 cut(s) 94, 664, 963, 1572
Tth111I GACNNNGTC 1 cut(s) 2101
VpaK11BI GGWCC 2 cut(s) 635, 1523
XagI CCTNNNNNAGG 1 cut(s) 2249
XapI RAATTY 3 cut(s) 325, 775, 1484
XhoI CTCGAG 1 cut(s) 2249
XmaJI CCTAGG 1 cut(s) 854
XmiI GTMKAC 1 cut(s) 1209
XmnI GAANNNNTTC 2 cut(s) 1388, 1607
XspI CTAG 5 cut(s) 284, 855, 951, 1271, 1752
Zsp2I ATGCAT 2 cut(s) 1963, 2051
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.