Serine threonine-protein kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 119 |
| Acc36I | ACCTGC | 1 cut(s) | 119 |
| AccI | GTMKAC | 2 cut(s) | 822, 2392 |
| AciI | CCGC | 2 cut(s) | 1681, 1974 |
| AclWI | GGATC | 3 cut(s) | 536, 582, 1983 |
| AcsI | RAATTY | 7 cut(s) | 594, 976, 1045, 1226, 1618, 1742, 2052 |
| AcuI | CTGAAG | 5 cut(s) | 243, 1257, 1658, 2316, 2412 |
| AfaI | GTAC | 4 cut(s) | 567, 954, 1147, 2094 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 51, 551, 1241 |
| AflIII | ACRYGT | 1 cut(s) | 2310 |
| AgeI | ACCGGT | 1 cut(s) | 500 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 90 |
| AjnI | CCWGG | 4 cut(s) | 160, 550, 1234, 2368 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 465, 497, 1536, 1568 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 46, 78 |
| Alw21I | GWGCWC | 2 cut(s) | 32, 536 |
| Alw26I | GTCTC | 2 cut(s) | 523, 2296 |
| AlwI | GGATC | 3 cut(s) | 536, 582, 1983 |
| Ama87I | CYCGRG | 1 cut(s) | 1842 |
| AoxI | GGCC | 1 cut(s) | 1808 |
| ApeKI | GCWGC | 5 cut(s) | 1188, 1268, 1341, 1527, 2365 |
| ApoI | RAATTY | 7 cut(s) | 594, 976, 1045, 1226, 1618, 1742, 2052 |
| AseI | ATTAAT | 3 cut(s) | 1367, 1577, 2207 |
| AsiGI | ACCGGT | 1 cut(s) | 500 |
| AspA2I | CCTAGG | 1 cut(s) | 49 |
| AspLEI | GCGC | 1 cut(s) | 896 |
| AspS9I | GGNCC | 3 cut(s) | 54, 1556, 1809 |
| AsuHPI | GGTGA | 8 cut(s) | 122, 176, 687, 1853, 1988, 2138, 2394, 2434 |
| AsuNHI | GCTAGC | 2 cut(s) | 1321, 1904 |
| AvaI | CYCGRG | 1 cut(s) | 1842 |
| AvaII | GGWCC | 2 cut(s) | 54, 1556 |
| AvrII | CCTAGG | 1 cut(s) | 49 |
| AxyI | CCTNAGG | 1 cut(s) | 793 |
| BaeGI | GKGCMC | 1 cut(s) | 1574 |
| BanII | GRGCYC | 1 cut(s) | 1851 |
| BbsI | GAAGAC | 4 cut(s) | 1461, 2252, 2296, 2418 |
| Bbv12I | GWGCWC | 2 cut(s) | 32, 536 |
| BbvI | GCAGC | 5 cut(s) | 1175, 1255, 1328, 1539, 2377 |
| BceAI | ACGGC | 2 cut(s) | 661, 1164 |
| BciT130I | CCWGG | 4 cut(s) | 162, 552, 1236, 2370 |
| BcoDI | GTCTC | 2 cut(s) | 523, 2296 |
| BfmI | CTRYAG | 2 cut(s) | 348, 852 |
| BfuAI | ACCTGC | 1 cut(s) | 119 |
| BglII | AGATCT | 1 cut(s) | 289 |
| BisI | GCNGC | 5 cut(s) | 1189, 1269, 1342, 1528, 2366 |
| BlnI | CCTAGG | 1 cut(s) | 49 |
| BlsI | GCNGC | 5 cut(s) | 1190, 1270, 1343, 1529, 2367 |
| Bme1390I | CCNGG | 4 cut(s) | 162, 552, 1236, 2370 |
| Bme18I | GGWCC | 2 cut(s) | 54, 1556 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 90 |
| BmeT110I | CYCGRG | 1 cut(s) | 1842 |
| BmgT120I | GGNCC | 3 cut(s) | 54, 1556, 1809 |
| BmiI | GGNNCC | 3 cut(s) | 55, 1850, 2031 |
| BmrFI | CCNGG | 4 cut(s) | 162, 552, 1236, 2370 |
| BmrI | ACTGGG | 1 cut(s) | 155 |
| BmsI | GCATC | 2 cut(s) | 2175, 2403 |
| BmtI | GCTAGC | 2 cut(s) | 1325, 1908 |
| BmuI | ACTGGG | 1 cut(s) | 155 |
| BpiI | GAAGAC | 4 cut(s) | 1461, 2252, 2296, 2418 |
| BpmI | CTGGAG | 2 cut(s) | 534, 1444 |
| BpuEI | CTTGAG | 3 cut(s) | 43, 757, 1127 |
| Bsa29I | ATCGAT | 1 cut(s) | 2408 |
| BsaAI | YACGTR | 1 cut(s) | 2225 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2148 |
| BsaI | GGTCTC | 1 cut(s) | 2296 |
| BsaJI | CCNNGG | 3 cut(s) | 49, 160, 1447 |
| BsaWI | WCCGGW | 1 cut(s) | 500 |
| BsaXI | ACNNNNNCTCC | 7 cut(s) | 70, 100, 279, 309, 2338, 2368, 2450 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 51, 551, 1241 |
| Bse118I | RCCGGY | 1 cut(s) | 500 |
| Bse1I | ACTGG | 4 cut(s) | 150, 1559, 1811, 2205 |
| Bse21I | CCTNAGG | 1 cut(s) | 793 |
| Bse3DI | GCAATG | 1 cut(s) | 2344 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2148 |
| BseBI | CCWGG | 4 cut(s) | 162, 552, 1236, 2370 |
| BseCI | ATCGAT | 1 cut(s) | 2408 |
| BseDI | CCNNGG | 3 cut(s) | 49, 160, 1447 |
| BseGI | GGATG | 6 cut(s) | 329, 576, 733, 1180, 1269, 2190 |
| BseJI | GATNNNNATC | 1 cut(s) | 2148 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 51, 551, 1241 |
| BseMI | GCAATG | 1 cut(s) | 2344 |
| BseMII | CTCAG | 5 cut(s) | 1281, 1351, 1420, 1704, 2394 |
| BseNI | ACTGG | 4 cut(s) | 150, 1559, 1811, 2205 |
| BseRI | GAGGAG | 3 cut(s) | 300, 645, 1124 |
| BseSI | GKGCMC | 1 cut(s) | 1574 |
| BseXI | GCAGC | 5 cut(s) | 1175, 1255, 1328, 1539, 2377 |
| BseYI | CCCAGC | 2 cut(s) | 804, 1537 |
| BsgI | GTGCAG | 1 cut(s) | 1327 |
| BshFI | GGCC | 1 cut(s) | 1810 |
| BshTI | ACCGGT | 1 cut(s) | 500 |
| BshVI | ATCGAT | 1 cut(s) | 2408 |
| BsiHKAI | GWGCWC | 2 cut(s) | 32, 536 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1842 |
| BsiSI | CCGG | 1 cut(s) | 501 |
| BslFI | GGGAC | 3 cut(s) | 67, 774, 1374 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 51, 551, 1241 |
| BsmAI | GTCTC | 2 cut(s) | 523, 2296 |
| BsmFI | GGGAC | 3 cut(s) | 67, 774, 1374 |
| BsnI | GGCC | 1 cut(s) | 1810 |
| Bso31I | GGTCTC | 1 cut(s) | 2296 |
| BsoBI | CYCGRG | 1 cut(s) | 1842 |
| Bsp1286I | GDGCHC | 4 cut(s) | 32, 536, 1574, 1851 |
| Bsp143I | GATC | 9 cut(s) | 280, 289, 541, 574, 761, 865, 1411, 1988, 2143 |
| BspACI | CCGC | 2 cut(s) | 1681, 1974 |
| BspANI | GGCC | 1 cut(s) | 1810 |
| BspCNI | CTCAG | 5 cut(s) | 1282, 1350, 1419, 1705, 2393 |
| BspDI | ATCGAT | 1 cut(s) | 2408 |
| BspHI | TCATGA | 1 cut(s) | 250 |
| BspLI | GGNNCC | 3 cut(s) | 55, 1850, 2031 |
| BspMAI | CTGCAG | 1 cut(s) | 352 |
| BspMI | ACCTGC | 1 cut(s) | 119 |
| BspOI | GCTAGC | 2 cut(s) | 1325, 1908 |
| BspPI | GGATC | 3 cut(s) | 536, 582, 1983 |
| BspQI | GCTCTTC | 1 cut(s) | 524 |
| BspTNI | GGTCTC | 1 cut(s) | 2296 |
| BsrDI | GCAATG | 1 cut(s) | 2344 |
| BsrFI | RCCGGY | 1 cut(s) | 500 |
| BsrI | ACTGG | 4 cut(s) | 150, 1559, 1811, 2205 |
| BssAI | RCCGGY | 1 cut(s) | 500 |
| BssECI | CCNNGG | 3 cut(s) | 49, 160, 1447 |
| BssMI | GATC | 9 cut(s) | 280, 289, 541, 574, 761, 865, 1411, 1988, 2143 |
| BssT1I | CCWWGG | 2 cut(s) | 49, 1447 |
| Bst2UI | CCWGG | 4 cut(s) | 162, 552, 1236, 2370 |
| Bst4CI | ACNGT | 3 cut(s) | 317, 856, 1348 |
| Bst6I | CTCTTC | 3 cut(s) | 252, 524, 2292 |
| BstBAI | YACGTR | 1 cut(s) | 2225 |
| BstC8I | GCNNGC | 5 cut(s) | 937, 941, 1193, 1323, 1906 |
| BstDEI | CTNAG | 9 cut(s) | 493, 615, 793, 1290, 1337, 1406, 1614, 1713, 2380 |
| BstEII | GGTNACC | 2 cut(s) | 1442, 2344 |
| BstF5I | GGATG | 6 cut(s) | 329, 576, 733, 1180, 1269, 2190 |
| BstHHI | GCGC | 1 cut(s) | 896 |
| BstKTI | GATC | 9 cut(s) | 283, 292, 544, 577, 764, 868, 1414, 1991, 2146 |
| BstMAI | GTCTC | 2 cut(s) | 523, 2296 |
| BstMBI | GATC | 9 cut(s) | 280, 289, 541, 574, 761, 865, 1411, 1988, 2143 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 1807 |
| BstNI | CCWGG | 4 cut(s) | 162, 552, 1236, 2370 |
| BstNSI | RCATGY | 3 cut(s) | 939, 2171, 2314 |
| BstPI | GGTNACC | 2 cut(s) | 1442, 2344 |
| BstSCI | CCNGG | 4 cut(s) | 160, 550, 1234, 2368 |
| BstSFI | CTRYAG | 2 cut(s) | 348, 852 |
| BstSLI | GKGCMC | 1 cut(s) | 1574 |
| BstV1I | GCAGC | 5 cut(s) | 1175, 1255, 1328, 1539, 2377 |
| BstV2I | GAAGAC | 4 cut(s) | 1461, 2252, 2296, 2418 |
| BstX2I | RGATCY | 3 cut(s) | 289, 541, 574 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 972 |
| BstYI | RGATCY | 3 cut(s) | 289, 541, 574 |
| Bsu15I | ATCGAT | 1 cut(s) | 2408 |
| Bsu36I | CCTNAGG | 1 cut(s) | 793 |
| BsuRI | GGCC | 1 cut(s) | 1810 |
| BsuTUI | ATCGAT | 1 cut(s) | 2408 |
| BtsCI | GGATG | 6 cut(s) | 329, 576, 733, 1180, 1269, 2190 |
| BtsI | GCAGTG | 1 cut(s) | 1033 |
| BtsIMutI | CAGTG | 4 cut(s) | 875, 1033, 1566, 1710 |
| BveI | ACCTGC | 1 cut(s) | 119 |
| Cac8I | GCNNGC | 5 cut(s) | 937, 941, 1193, 1323, 1906 |
| CciI | TCATGA | 1 cut(s) | 250 |
| CfoI | GCGC | 1 cut(s) | 896 |
| Cfr10I | RCCGGY | 1 cut(s) | 500 |
| Cfr13I | GGNCC | 3 cut(s) | 54, 1556, 1809 |
| ClaI | ATCGAT | 1 cut(s) | 2408 |
| Csp6I | GTAC | 4 cut(s) | 566, 953, 1146, 2093 |
| CspAI | ACCGGT | 1 cut(s) | 500 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1986, 2021 |
| CviQI | GTAC | 4 cut(s) | 566, 953, 1146, 2093 |
| DdeI | CTNAG | 9 cut(s) | 493, 615, 793, 1290, 1337, 1406, 1614, 1713, 2380 |
| DpnI | GATC | 9 cut(s) | 282, 291, 543, 576, 763, 867, 1413, 1990, 2145 |
| DpnII | GATC | 9 cut(s) | 280, 289, 541, 574, 761, 865, 1411, 1988, 2143 |
| DraI | TTTAAA | 1 cut(s) | 270 |
| DriI | GACNNNNNGTC | 1 cut(s) | 90 |
| Eam1104I | CTCTTC | 3 cut(s) | 252, 524, 2292 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 90 |
| EarI | CTCTTC | 3 cut(s) | 252, 524, 2292 |
| Eco130I | CCWWGG | 2 cut(s) | 49, 1447 |
| Eco24I | GRGCYC | 1 cut(s) | 1851 |
| Eco31I | GGTCTC | 1 cut(s) | 2296 |
| Eco32I | GATATC | 2 cut(s) | 671, 1168 |
| Eco47I | GGWCC | 2 cut(s) | 54, 1556 |
| Eco57I | CTGAAG | 5 cut(s) | 243, 1257, 1658, 2316, 2412 |
| Eco81I | CCTNAGG | 1 cut(s) | 793 |
| Eco88I | CYCGRG | 1 cut(s) | 1842 |
| Eco91I | GGTNACC | 2 cut(s) | 1442, 2344 |
| EcoO109I | RGGNCCY | 1 cut(s) | 54 |
| EcoO65I | GGTNACC | 2 cut(s) | 1442, 2344 |
| EcoRI | GAATTC | 4 cut(s) | 976, 1226, 1618, 1742 |
| EcoRII | CCWGG | 4 cut(s) | 160, 550, 1234, 2368 |
| EcoRV | GATATC | 2 cut(s) | 671, 1168 |
| EcoT14I | CCWWGG | 2 cut(s) | 49, 1447 |
| EcoT22I | ATGCAT | 2 cut(s) | 2173, 2418 |
| EcoT38I | GRGCYC | 1 cut(s) | 1851 |
| ErhI | CCWWGG | 2 cut(s) | 49, 1447 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1431, 1463 |
| FaqI | GGGAC | 3 cut(s) | 67, 774, 1374 |
| FauNDI | CATATG | 2 cut(s) | 1153, 2017 |
| FblI | GTMKAC | 2 cut(s) | 822, 2392 |
| Fnu4HI | GCNGC | 5 cut(s) | 1189, 1269, 1342, 1528, 2366 |
| FokI | GGATG | 6 cut(s) | 316, 583, 740, 1187, 1276, 2197 |
| FriOI | GRGCYC | 1 cut(s) | 1851 |
| Fsp4HI | GCNGC | 5 cut(s) | 1189, 1269, 1342, 1528, 2366 |
| GlaI | GCGC | 1 cut(s) | 895 |
| GluI | GCNGC | 5 cut(s) | 1189, 1269, 1342, 1528, 2366 |
| GsaI | CCCAGC | 2 cut(s) | 808, 1541 |
| GsuI | CTGGAG | 2 cut(s) | 534, 1444 |
| HaeIII | GGCC | 1 cut(s) | 1810 |
| HapII | CCGG | 1 cut(s) | 501 |
| HhaI | GCGC | 1 cut(s) | 896 |
| Hin6I | GCGC | 1 cut(s) | 894 |
| HinP1I | GCGC | 1 cut(s) | 894 |
| HincII | GTYRAC | 1 cut(s) | 823 |
| HindII | GTYRAC | 1 cut(s) | 823 |
| HindIII | AAGCTT | 3 cut(s) | 479, 1694, 1759 |
| HpaII | CCGG | 1 cut(s) | 501 |
| HphI | GGTGA | 8 cut(s) | 122, 176, 687, 1853, 1988, 2138, 2394, 2434 |
| Hpy166II | GTNNAC | 4 cut(s) | 680, 823, 1006, 2393 |
| Hpy188I | TCNGA | 9 cut(s) | 84, 457, 742, 1276, 1948, 2071, 2245, 2383, 2431 |
| Hpy8I | GTNNAC | 4 cut(s) | 680, 823, 1006, 2393 |
| HpyAV | CCTTC | 7 cut(s) | 55, 308, 462, 601, 1538, 1868, 2168 |
| HpyCH4III | ACNGT | 3 cut(s) | 317, 856, 1348 |
| HpyCH4IV | ACGT | 2 cut(s) | 2219, 2224 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 1807 |
| HpyF3I | CTNAG | 9 cut(s) | 493, 615, 793, 1290, 1337, 1406, 1614, 1713, 2380 |
| HpySE526I | ACGT | 2 cut(s) | 2219, 2224 |
| HspAI | GCGC | 1 cut(s) | 894 |
| Kzo9I | GATC | 9 cut(s) | 280, 289, 541, 574, 761, 865, 1411, 1988, 2143 |
| LguI | GCTCTTC | 1 cut(s) | 524 |
| LmnI | GCTCC | 5 cut(s) | 135, 553, 611, 1854, 2035 |
| Lsp1109I | GCAGC | 5 cut(s) | 1175, 1255, 1328, 1539, 2377 |
| LweI | GCATC | 2 cut(s) | 2175, 2403 |
| MaeII | ACGT | 2 cut(s) | 2219, 2224 |
| MaeIII | GTNAC | 7 cut(s) | 211, 496, 655, 1442, 1502, 2220, 2344 |
| MalI | GATC | 9 cut(s) | 282, 291, 543, 576, 763, 867, 1413, 1990, 2145 |
| MboI | GATC | 9 cut(s) | 280, 289, 541, 574, 761, 865, 1411, 1988, 2143 |
| MflI | RGATCY | 3 cut(s) | 289, 541, 574 |
| MhlI | GDGCHC | 4 cut(s) | 32, 536, 1574, 1851 |
| MlyI | GAGTC | 6 cut(s) | 79, 796, 1006, 1862, 2398, 2468 |
| MmeI | TCCRAC | 2 cut(s) | 467, 2436 |
| Mph1103I | ATGCAT | 2 cut(s) | 2173, 2418 |
| MspA1I | CMGCKG | 1 cut(s) | 1341 |
| MspI | CCGG | 1 cut(s) | 501 |
| MspR9I | CCNGG | 4 cut(s) | 162, 552, 1236, 2370 |
| MvaI | CCWGG | 4 cut(s) | 162, 552, 1236, 2370 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 1807 |
| NdeI | CATATG | 2 cut(s) | 1153, 2017 |
| NdeII | GATC | 9 cut(s) | 280, 289, 541, 574, 761, 865, 1411, 1988, 2143 |
| NheI | GCTAGC | 2 cut(s) | 1321, 1904 |
| NlaIV | GGNNCC | 3 cut(s) | 55, 1850, 2031 |
| NmuCI | GTSAC | 1 cut(s) | 211 |
| NsiI | ATGCAT | 2 cut(s) | 2173, 2418 |
| NspI | RCATGY | 3 cut(s) | 939, 2171, 2314 |
| PaeI | GCATGC | 1 cut(s) | 939 |
| PaeR7I | CTCGAG | 1 cut(s) | 1842 |
| PagI | TCATGA | 1 cut(s) | 250 |
| PaqCI | CACCTGC | 1 cut(s) | 119 |
| PciI | ACATGT | 1 cut(s) | 2310 |
| PciSI | GCTCTTC | 1 cut(s) | 524 |
| PfeI | GAWTC | 7 cut(s) | 150, 254, 355, 377, 2130, 2228, 2399 |
| PfoI | TCCNGGA | 1 cut(s) | 550 |
| PinAI | ACCGGT | 1 cut(s) | 500 |
| PkrI | GCNGC | 5 cut(s) | 1190, 1270, 1343, 1529, 2367 |
| PleI | GAGTC | 6 cut(s) | 79, 795, 1006, 1862, 2397, 2467 |
| PpsI | GAGTC | 6 cut(s) | 79, 795, 1006, 1862, 2397, 2467 |
| Ppu21I | YACGTR | 1 cut(s) | 2225 |
| PpuMI | RGGWCCY | 1 cut(s) | 54 |
| PscI | ACATGT | 1 cut(s) | 2310 |
| PshBI | ATTAAT | 3 cut(s) | 1367, 1577, 2207 |
| Psp5II | RGGWCCY | 1 cut(s) | 54 |
| Psp6I | CCWGG | 4 cut(s) | 160, 550, 1234, 2368 |
| PspEI | GGTNACC | 2 cut(s) | 1442, 2344 |
| PspFI | CCCAGC | 2 cut(s) | 804, 1537 |
| PspGI | CCWGG | 4 cut(s) | 160, 550, 1234, 2368 |
| PspN4I | GGNNCC | 3 cut(s) | 55, 1850, 2031 |
| PspPI | GGNCC | 3 cut(s) | 54, 1556, 1809 |
| PspPPI | RGGWCCY | 1 cut(s) | 54 |
| PspXI | VCTCGAGB | 1 cut(s) | 1842 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 2376, 2408 |
| PstI | CTGCAG | 1 cut(s) | 352 |
| PsuI | RGATCY | 3 cut(s) | 289, 541, 574 |
| PvuII | CAGCTG | 1 cut(s) | 1341 |
| RsaI | GTAC | 4 cut(s) | 567, 954, 1147, 2094 |
| RsaNI | GTAC | 4 cut(s) | 566, 953, 1146, 2093 |
| SalI | GTCGAC | 1 cut(s) | 821 |
| SapI | GCTCTTC | 1 cut(s) | 524 |
| SatI | GCNGC | 5 cut(s) | 1189, 1269, 1342, 1528, 2366 |
| Sau3AI | GATC | 9 cut(s) | 280, 289, 541, 574, 761, 865, 1411, 1988, 2143 |
| Sau96I | GGNCC | 3 cut(s) | 54, 1556, 1809 |
| SchI | GAGTC | 6 cut(s) | 79, 796, 1006, 1862, 2398, 2468 |
| ScrFI | CCNGG | 4 cut(s) | 162, 552, 1236, 2370 |
| SduI | GDGCHC | 4 cut(s) | 32, 536, 1574, 1851 |
| SfaNI | GCATC | 2 cut(s) | 2175, 2403 |
| SfcI | CTRYAG | 2 cut(s) | 348, 852 |
| Sfr274I | CTCGAG | 1 cut(s) | 1842 |
| SinI | GGWCC | 2 cut(s) | 54, 1556 |
| SlaI | CTCGAG | 1 cut(s) | 1842 |
| SmlI | CTYRAG | 4 cut(s) | 58, 772, 1106, 1842 |
| SmoI | CTYRAG | 4 cut(s) | 58, 772, 1106, 1842 |
| SphI | GCATGC | 1 cut(s) | 939 |
| SsiI | CCGC | 2 cut(s) | 1681, 1974 |
| SspI | AATATT | 1 cut(s) | 1912 |
| StyD4I | CCNGG | 4 cut(s) | 160, 550, 1234, 2368 |
| StyI | CCWWGG | 2 cut(s) | 49, 1447 |
| TaaI | ACNGT | 3 cut(s) | 317, 856, 1348 |
| TaiI | ACGT | 2 cut(s) | 2222, 2227 |
| TaqI | TCGA | 7 cut(s) | 283, 311, 822, 1803, 1843, 1920, 2408 |
| TatI | WGTACW | 1 cut(s) | 2092 |
| TfiI | GAWTC | 7 cut(s) | 150, 254, 355, 377, 2130, 2228, 2399 |
| TscAI | CASTG | 4 cut(s) | 875, 1033, 1566, 1717 |
| TseFI | GTSAC | 1 cut(s) | 211 |
| TseI | GCWGC | 5 cut(s) | 1188, 1268, 1341, 1527, 2365 |
| Tsp45I | GTSAC | 1 cut(s) | 211 |
| TspGWI | ACGGA | 3 cut(s) | 583, 1276, 2401 |
| TspRI | CASTG | 4 cut(s) | 875, 1033, 1566, 1717 |
| VpaK11BI | GGWCC | 2 cut(s) | 54, 1556 |
| VspI | ATTAAT | 3 cut(s) | 1367, 1577, 2207 |
| XapI | RAATTY | 7 cut(s) | 594, 976, 1045, 1226, 1618, 1742, 2052 |
| XbaI | TCTAGA | 1 cut(s) | 1871 |
| XceI | RCATGY | 3 cut(s) | 939, 2171, 2314 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 1759, 2452 |
| XhoI | CTCGAG | 1 cut(s) | 1842 |
| XmaJI | CCTAGG | 1 cut(s) | 49 |
| XmiI | GTMKAC | 2 cut(s) | 822, 2392 |
| Zsp2I | ATGCAT | 2 cut(s) | 2173, 2418 |