MD05G1218000.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000207
Physical Location & Seq
Forward (+)
34869842 .. 34873359
3518 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1218000.v1.1.491

Sequence Viewer

Length: 2601 bp
ATGTACAGTTTCAGAAATATGACAATGCAGTCCTTGAGAAGCATTTTTGTGTGTTTGTTCTTATTATCCTTCCTAAGGATCTCATCTGCAGCTACACTAGGCACTATCACTCGTAGTCAATATATTAGAGATGGCGAAACTCTGGTTTCAGCAGGTGGAGGCTTTGAACTGGGATTTTTCAGCCCTGGTAACTCGAAGGGCCGGTACTTGGGGATATGGTACACAGTTTCTGCTGATACAGTTGTTTGGGTAGCCAACAGAGAAACACCACTCGGTGATTCGTCCGGGTTTTTGAAGCTCAGCGAGCAAGGAGTACTAGTCCTCCTTAATAGCTCAGACAACATTGTATGGTCATCGAATTCATCAAGAACCGTGGGGAATCCAGTATCACAACTCTTGGATACTGGAAATCTTATTGTGAAAGACGGAAATGAAACGAACTCGGATAACTTCTCGTGGCAGAGTTTTGATTACCCTTGTGACACATTCCTACCGGAAATGAAGCTTGGTTGGGACTTAGCTAGCGATTTAGAGAGGTATGTCTCGTCTTGGAAGAGCACAGAAAATCCTGCTCCAGGAGATTTTTCACTACGGATAAGTCGTTGGGGCTTACCACAACTTGTTACTATGAAAGGAGCTAAGATACAGGCTAGAGGAGGGTCATGGAATGGTGTTCATTTTACAGGATATGGATCTAGTGGAATAAGAAACTCCAATCCATTACCTGGGGTTGAATTTCGGTTGAACAAGAATGAGGTCTACTATGAGTACAGGCTCCTAAACAGGTCGGAGTTCTCGAGATATGTATTGAACCCGTTAGGCATCACACAACGGTTCACATGGGTGTACCAAACACAGAGTTGGGAACTTTCCTTTACATTCCAAGCAGATCAGTGTGAAAATTATGCCTTGTGTGGTGCATATGCTACTTGCAATATCAACAACGCTCCAATATGTGCATGCTTGAAGGGATTTGCACCAAAATCTCCAAAAGATTGGAACTCGGGATACTGGTCCGGTGGGTGCGTTCGAAAGACTCCGTTGGCCTGCAGCCATGGAGACGGCTTCCTAAAGTATAATGGGATTAAATTGCCAGACACATCTACCTCATGGTTTAATAAAAGCATGAGCCTCAAGGAATGCAAGTGGTTATGTTTGGAAAACTGCTCATGTACGGCGTATGCAAATCTTGATATCAGGGTGGGAGGAAGTGGCTGCTTGCTTTGGTTTGGCAACCTCATTGACATAAGAGAATTCGCTATAAGTGACGTTCAGGACCTCTATATACGGATGGCAGCTTCAGAACTAGATCACTTTGTGAAAGAGAGCAAGTTCAACAAGAAAAAACTACCAATTCTCATCAGCTTCGCAGTTTTACTCCTGGGAACGATTATAGTTGGATTGATATTGTACATACGGAAGAAGAAACTCAGAAATGAAGGAGTCAGAAGAAATGATTTCAGAAAAGAGTATCTTGGAGAAGACAGGGAGTTACCACTATTTGACTTGACCACCATAGCCAAAGCAACTGATAACTTTTCAAGCAGCAACAAGTTGGGAGAAGGTGGTTTTGGACCTGTGTACAAGGGCACATTGATGGGAGGGAAAGAAGTTGCAGTTAAGAGACTATCGAAGGATTCTGGACAAGGAATGAGGGAGTTCAAAAATGAAGTAAAAATGATAGCCAGACTTCAGCATCGGAATCTAGTAAAACTTCTGGGCTGTTGCACTCAAGAAGATGAAAAGATTCTAATATATGAATTCATGCCTAATAGAAGCTTAGACTTCTTTGTTTTTGGTATGATTCCCTCCAAAACCAAACATGTGGCTTTTAGATCATACCATCCTTCACCTTTTAAACTTATTTTACCAGGATTCAATGTGACACTAATTAACATGCTTAAAGTTTCTTCAGATGAAGAGGGACGAAAATTGCTCAACTGGACTAAGTGCTTCCACATTATTGGTGGAATTGCTCGGGGACTTGTTTATCTTCACCATGACTCTAGATTAAGGGTTATCCATCGAGATCTGAAAGCTAGCAATATTCTGCTTGATAATAATTTGAACCCAAAGATTGCAGACTTTGGCTTGGCTAAAATATTCGACAGTGATCAAAGTCAGGCCAGTACAAATAGAGTGGTGGGAACCTATGGTTATATGTCTCCCGAATATGCTGTAGATGGAATTTTCTCAATGAAATCGGATGTCTTCAGCTTTGGAGTTATACTGCTAGAGATGTTGTGTAGGAAGAAGAACAGGGGTTTTTGTCATCCGGATCACCACCTTAACCTTCTTGGACATGCATGGATGCTATGGATGCAAGATAAACAACTGGAATTGATCGATAAGACTCTTTCTGATTCGTGCAATATATATGAAGTGGTAAGGTGTCTTCATGTGGGGCTGTTATGTGTGCAAAGAGTACCTGAGGATAGACCAAGCATGTCATCTGTGGTTCTGATGTTGAGCAGTGATGTTGCCTTGCCTTCTCCAAAGCAGCCTGGTTTTTACACTGAACGAAGTGTCTCTGAATCACCATCAAGCAAGCGTCAATGTTCAGAGAATGATGTCAGCATTTCATTGATAGAACCTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

867

Amino Acids

97.46

Weight (kDa)

8.81

Isoelectric Point (pI)

43.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 80 - 185 3.4e-37 D-mannose binding lectin
S_locus_glycop PF00954 218 - 329 2.4e-25 S-locus glycoprotein domain
PAN_2 PF08276 350 - 416 1.8e-22 PAN-like domain
Pkinase PF00069 514 - 597 3.2e-13 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 516 - 820 1.7e-45 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 645 - 754 7.2e-27 Protein kinase domain
DUF3403 PF11883 824 - 866 3.6e-12 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27300
fragaria_vesca FvH4_3g15930 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g21350 FvH4_3g21400 FvH4_3g21400 FvH4_6g12332
malus_domestica MD02G1167100.v1.1 MD02G1167200.v1.1 MD03G1185600.v1.1 MD05G1213900.v1.1 MD05G1214100.v1.1 MD05G1214200.v1.1 MD05G1214700.v1.1 MD05G1216300.v1.1 MD05G1216800.v1.1 MD05G1217300.v1.1 MD05G1218000.v1.1 MD11G1231100.v1.1 MD11G1231200.v1.1 MD11G1231400.v1.1 MD11G1231500.v1.1 MD11G1232400.v1.1 MD11G1232500.v1.1
prunus_persica Prupe.4G142300_v2.0.a1 Prupe.4G142400_v2.0.a1 Prupe.4G142500_v2.0.a1 Prupe.4G142600_v2.0.a1 Prupe.4G142800_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195100_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195500_v2.0.a1 Prupe.4G195700_v2.0.a1 Prupe.4G195900_v2.0.a1
pyrus_communis pycom03g16540 pycom05g19920 pycom11g20460 pycom11g20480 pycom11g20490 pycom11g20500 pycom11g20530
rosa_chinensis RchiOBHm_Chr2g0139311 RchiOBHm_Chr2g0139351 RchiOBHm_Chr5g0026801 RchiOBHm_Chr5g0026851 RchiOBHm_Chr5g0026891 RchiOBHm_Chr5g0026911 RchiOBHm_Chr5g0036571 RchiOBHm_Chr5g0036591 RchiOBHm_Chr5g0036721 RchiOBHm_Chr5g0036741 RchiOBHm_Chr7g0222981
rosa_laevigata RLG00000002006 RLG00000019807 RLG00000032964 RLG00000032968 RLG00000032969 RLG00000033706 RLG00000033708 RLG00000033712 RLG00000033716
rosa_multiflora Rmu_co8256651.1_g000001 Rmu_co8279377.1_g000001 Rmu_co8429919.1_g000001 Rmu_co8490103.1_g000001 Rmu_sc0000774.1_g000002 Rmu_sc0002525.1_g000012 Rmu_sc0004223.1_g000008 Rmu_sc0004390.1_g000002 Rmu_sc0006059.1_g000014 Rmu_sc0006059.1_g000024 Rmu_sc0006514.1_g000020 Rmu_sc0014333.1_g000002 Rmu_ssc0000172.1_g000018 Rmu_ssc0000172.1_g000029
rosa_roxburghii Rroxscaffold_1G00044230 Rroxscaffold_1G00044260 Rroxscaffold_1G00044320 Rroxscaffold_1G00044340 Rroxscaffold_1G00052560 Rroxscaffold_1G00052570 Rroxscaffold_1G00052660 Rroxscaffold_1G00052720 Rroxscaffold_2G00105450 Rroxscaffold_2G00105460
rosa_rugosa Rorug02G0356900 Rorug02G0357000 Rorug02G0357100 Rorug02G0357200 Rorug02G0357300 Rorug02G0357400 Rorug02G0357500 Rorug02G0357600 Rorug05G0095400 Rorug05G0095500 Rorug05G0095500 Rorug05G0095600 Rorug05G0095700 Rorug05G0095800 Rorug05G0157700 Rorug05G0157800
rosa_samantha Rh2AG406800 Rh2AG406900 Rh2BG417300 Rh2CG392900 Rh2DG426200 Rh5AG189300 Rh5AG249400 Rh5AG250100 Rh5BG186300 Rh5BG186900 Rh5BG187000 Rh5BG249500 Rh5BG250600 Rh5BG250900 Rh5BG251500 Rh5BG251800 Rh5CG205500 Rh5CG206300 Rh5CG206700 Rh5CG283000 Rh5CG283300 Rh5CG283500 Rh5DG188500 Rh5DG188700 Rh5DG258700 Rh5DG258900 Rh5DG259600 Rh5DG259900 Rh7CG375800 Rh7DG352500
rosa_wichuraiana Rw2G032960 Rw2G032970 Rw2G033220 Rw2G033230 Rw5G017130 Rw5G017230 Rw5G022940 Rw5G022960 Rw5G022980 Rw7G030420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 143
AasI GACNNNNNNGTC 1 cut(s) 28
Acc36I ACCTGC 1 cut(s) 143
AccB7I CCANNNNNTGG 1 cut(s) 725
AccI GTMKAC 1 cut(s) 759
AccIII TCCGGA 1 cut(s) 2275
AclWI GGATC 3 cut(s) 86, 700, 2286
AcsI RAATTY 5 cut(s) 358, 734, 1253, 1760, 2187
AcuI CTGAAG 4 cut(s) 1284, 1676, 1896, 2197
AdeI CACNNNGTG 2 cut(s) 275, 1318
AfiI CCNNNNNNNGG 4 cut(s) 75, 208, 575, 725
AflIII ACRYGT 1 cut(s) 1822
AhlI ACTAGT 1 cut(s) 316
AjnI CCWGG 6 cut(s) 184, 574, 724, 1380, 1870, 2503
AjuI GAANNNNNNNTTGG 4 cut(s) 489, 521, 1554, 1586
AleI CACNNNNGTG 1 cut(s) 842
Alw21I GWGCWC 1 cut(s) 560
Alw26I GTCTC 5 cut(s) 547, 1053, 1618, 2169, 2533
AlwI GGATC 3 cut(s) 86, 700, 2286
AlwNI CAGNNNCTG 1 cut(s) 230
Ama87I CYCGRG 3 cut(s) 796, 1003, 1977
Aor13HI TCCGGA 1 cut(s) 2275
AoxI GGCC 3 cut(s) 199, 1044, 2124
ApeKI GCWGC 6 cut(s) 89, 1050, 1215, 1295, 1545, 2500
ApoI RAATTY 5 cut(s) 358, 734, 1253, 1760, 2187
Asp700I GAANNNNTTC 1 cut(s) 1746
AspS9I GGNCC 4 cut(s) 199, 1014, 1276, 1574
AsuC2I CCSGG 1 cut(s) 286
AsuHPI GGTGA 5 cut(s) 287, 1842, 1988, 2273, 2529
AsuII TTCGAA 1 cut(s) 1030
AsuNHI GCTAGC 2 cut(s) 521, 2039
AvaI CYCGRG 3 cut(s) 796, 1003, 1977
AvaII GGWCC 3 cut(s) 1014, 1276, 1574
AxyI CCTNAGG 2 cut(s) 74, 2430
BaeGI GKGCMC 1 cut(s) 1592
BaeI ACNNNNGTAYC 2 cut(s) 228, 261
BarI GAAGNNNNNNTAC 4 cut(s) 188, 220, 2379, 2411
BauI CACGAG 1 cut(s) 454
BbsI GAAGAC 3 cut(s) 1488, 2203, 2387
Bbv12I GWGCWC 1 cut(s) 560
BbvI GCAGC 6 cut(s) 101, 1062, 1202, 1307, 1557, 2512
BccI CCATC 7 cut(s) 125, 1285, 1591, 1851, 2031, 2177, 2548
BceAI ACGGC 2 cut(s) 1078, 1191
BciT130I CCWGG 6 cut(s) 186, 576, 726, 1382, 1872, 2505
BciVI GTATCC 2 cut(s) 394, 1001
BclI TGATCA 1 cut(s) 2113
BcnI CCSGG 1 cut(s) 286
BcoDI GTCTC 5 cut(s) 547, 1053, 1618, 2169, 2533
BcuI ACTAGT 1 cut(s) 316
BfmI CTRYAG 3 cut(s) 87, 1048, 2178
BfuAI ACCTGC 1 cut(s) 143
BfuI GTATCC 2 cut(s) 394, 1001
BglII AGATCT 1 cut(s) 2029
BisI GCNGC 6 cut(s) 90, 1051, 1216, 1296, 1546, 2501
BlpI GCTNAGC 1 cut(s) 299
BlsI GCNGC 6 cut(s) 91, 1052, 1217, 1297, 1547, 2502
BmcAI AGTACT 1 cut(s) 315
Bme1390I CCNGG 7 cut(s) 186, 286, 576, 726, 1382, 1872, 2505
Bme18I GGWCC 3 cut(s) 1014, 1276, 1574
BmeT110I CYCGRG 3 cut(s) 796, 1003, 1977
BmgT120I GGNCC 4 cut(s) 199, 1014, 1276, 1574
BmiI GGNNCC 2 cut(s) 776, 2149
BmrFI CCNGG 7 cut(s) 186, 286, 576, 726, 1382, 1872, 2505
BmrI ACTGGG 1 cut(s) 179
BmsI GCATC 4 cut(s) 831, 1705, 2301, 2310
BmtI GCTAGC 2 cut(s) 525, 2043
BmuI ACTGGG 1 cut(s) 179
BpiI GAAGAC 3 cut(s) 1488, 2203, 2387
BpmI CTGGAG 1 cut(s) 558
Bpu1102I GCTNAGC 1 cut(s) 299
Bpu14I TTCGAA 1 cut(s) 1030
BpuEI CTTGAG 3 cut(s) 55, 1118, 1716
BpuMI CCSGG 1 cut(s) 286
Bsa29I ATCGAT 1 cut(s) 2346
BsaBI GATNNNNATC 1 cut(s) 691
BsaJI CCNNGG 6 cut(s) 184, 372, 725, 1054, 1381, 2594
BsaWI WCCGGW 3 cut(s) 493, 1016, 2275
BsaXI ACNNNNNCTCC 4 cut(s) 303, 306, 333, 336
Bsc4I CCNNNNNNNGG 4 cut(s) 75, 208, 575, 725
Bse118I RCCGGY 1 cut(s) 201
Bse1I ACTGG 7 cut(s) 174, 383, 409, 1016, 1946, 2127, 2340
Bse21I CCTNAGG 2 cut(s) 74, 2430
Bse8I GATNNNNATC 1 cut(s) 691
BseAI TCCGGA 1 cut(s) 2275
BseBI CCWGG 6 cut(s) 186, 576, 726, 1382, 1872, 2505
BseCI ATCGAT 1 cut(s) 2346
BseDI CCNNGG 6 cut(s) 184, 372, 725, 1054, 1381, 2594
BseGI GGATG 6 cut(s) 1296, 1843, 2212, 2272, 2316, 2325
BseJI GATNNNNATC 1 cut(s) 691
BseLI CCNNNNNNNGG 4 cut(s) 75, 208, 575, 725
BseMII CTCAG 4 cut(s) 313, 348, 1444, 2421
BseNI ACTGG 7 cut(s) 174, 383, 409, 1016, 1946, 2127, 2340
BseRI GAGGAG 1 cut(s) 669
BseSI GKGCMC 1 cut(s) 1592
BseXI GCAGC 6 cut(s) 101, 1062, 1202, 1307, 1557, 2512
BshFI GGCC 3 cut(s) 201, 1046, 2126
BshVI ATCGAT 1 cut(s) 2346
BsiHKAI GWGCWC 1 cut(s) 560
BsiHKCI CYCGRG 3 cut(s) 796, 1003, 1977
BsiSI CCGG 5 cut(s) 202, 285, 494, 1017, 2276
BslFI GGGAC 3 cut(s) 527, 1938, 1995
BslI CCNNNNNNNGG 4 cut(s) 75, 208, 575, 725
BsmAI GTCTC 5 cut(s) 547, 1053, 1618, 2169, 2533
BsmBI CGTCTC 1 cut(s) 1053
BsmFI GGGAC 3 cut(s) 527, 1938, 1995
BsmI GAATGC 1 cut(s) 1145
BsnI GGCC 3 cut(s) 201, 1046, 2126
BsoBI CYCGRG 3 cut(s) 796, 1003, 1977
Bsp119I TTCGAA 1 cut(s) 1030
Bsp1286I GDGCHC 2 cut(s) 560, 1592
Bsp13I TCCGGA 1 cut(s) 2275
Bsp1407I TGTACA 3 cut(s) 3, 1410, 1581
Bsp143I GATC 9 cut(s) 78, 692, 889, 1309, 1835, 2029, 2113, 2278, 2343
Bsp1720I GCTNAGC 1 cut(s) 299
Bsp19I CCATGG 1 cut(s) 1054
BspANI GGCC 3 cut(s) 201, 1046, 2126
BspCNI CTCAG 4 cut(s) 312, 347, 1443, 2422
BspDI ATCGAT 1 cut(s) 2346
BspEI TCCGGA 1 cut(s) 2275
BspLI GGNNCC 2 cut(s) 776, 2149
BspMAI CTGCAG 2 cut(s) 91, 1052
BspMI ACCTGC 1 cut(s) 143
BspOI GCTAGC 2 cut(s) 525, 2043
BspPI GGATC 3 cut(s) 86, 700, 2286
BspQI GCTCTTC 1 cut(s) 548
BspT104I TTCGAA 1 cut(s) 1030
BsrFI RCCGGY 1 cut(s) 201
BsrGI TGTACA 3 cut(s) 3, 1410, 1581
BsrI ACTGG 7 cut(s) 174, 383, 409, 1016, 1946, 2127, 2340
BssAI RCCGGY 1 cut(s) 201
BssECI CCNNGG 6 cut(s) 184, 372, 725, 1054, 1381, 2594
BssMI GATC 9 cut(s) 78, 692, 889, 1309, 1835, 2029, 2113, 2278, 2343
BssSI CACGAG 1 cut(s) 454
BssT1I CCWWGG 1 cut(s) 1054
Bst2BI CACGAG 1 cut(s) 454
Bst2UI CCWGG 6 cut(s) 186, 576, 726, 1382, 1872, 2505
Bst4CI ACNGT 6 cut(s) 8, 226, 241, 373, 834, 2111
Bst6I CTCTTC 2 cut(s) 548, 1914
BstAUI TGTACA 3 cut(s) 3, 1410, 1581
BstBI TTCGAA 1 cut(s) 1030
BstC8I GCNNGC 7 cut(s) 305, 523, 961, 1048, 1220, 2041, 2549
BstDEI CTNAG 9 cut(s) 74, 299, 334, 517, 639, 1430, 1780, 1947, 2430
BstDSI CCRYGG 2 cut(s) 372, 1054
BstENI CCTNNNNNAGG 2 cut(s) 73, 573
BstF5I GGATG 6 cut(s) 1296, 1843, 2212, 2272, 2316, 2325
BstKTI GATC 9 cut(s) 81, 695, 892, 1312, 1838, 2032, 2116, 2281, 2346
BstMAI GTCTC 5 cut(s) 547, 1053, 1618, 2169, 2533
BstMBI GATC 9 cut(s) 78, 692, 889, 1309, 1835, 2029, 2113, 2278, 2343
BstMWI GCNNNNNNNGC 2 cut(s) 304, 2320
BstNI CCWGG 6 cut(s) 186, 576, 726, 1382, 1872, 2505
BstNSI RCATGY 5 cut(s) 963, 1826, 1900, 2306, 2449
BstSCI CCNGG 7 cut(s) 184, 284, 574, 724, 1380, 1870, 2503
BstSFI CTRYAG 3 cut(s) 87, 1048, 2178
BstSLI GKGCMC 1 cut(s) 1592
BstV1I GCAGC 6 cut(s) 101, 1062, 1202, 1307, 1557, 2512
BstV2I GAAGAC 3 cut(s) 1488, 2203, 2387
BstX2I RGATCY 3 cut(s) 78, 692, 2029
BstXI CCANNNNNNTGG 3 cut(s) 996, 1825, 1964
BstYI RGATCY 3 cut(s) 78, 692, 2029
Bsu15I ATCGAT 1 cut(s) 2346
Bsu36I CCTNAGG 2 cut(s) 74, 2430
BsuI GTATCC 2 cut(s) 394, 1001
BsuRI GGCC 3 cut(s) 201, 1046, 2126
BsuTUI ATCGAT 1 cut(s) 2346
BtgI CCRYGG 2 cut(s) 372, 1054
BtsCI GGATG 6 cut(s) 1296, 1843, 2212, 2272, 2316, 2325
BtsI GCAGTG 1 cut(s) 2479
BtsIMutI CAGTG 4 cut(s) 899, 2116, 2479, 2514
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 7 cut(s) 305, 523, 961, 1048, 1220, 2041, 2549
CaiI CAGNNNCTG 1 cut(s) 230
Cfr10I RCCGGY 1 cut(s) 201
Cfr13I GGNCC 4 cut(s) 199, 1014, 1276, 1574
ClaI ATCGAT 1 cut(s) 2346
CseI GACGC 1 cut(s) 2540
CspCI CAANNNNNGTGG 4 cut(s) 354, 389, 2121, 2156
DdeI CTNAG 9 cut(s) 74, 299, 334, 517, 639, 1430, 1780, 1947, 2430
DpnI GATC 9 cut(s) 80, 694, 891, 1311, 1837, 2031, 2115, 2280, 2345
DpnII GATC 9 cut(s) 78, 692, 889, 1309, 1835, 2029, 2113, 2278, 2343
DraI TTTAAA 1 cut(s) 1858
DraIII CACNNNGTG 2 cut(s) 275, 1318
DrdI GACNNNNNNGTC 1 cut(s) 28
DseDI GACNNNNNNGTC 1 cut(s) 28
Eam1104I CTCTTC 2 cut(s) 548, 1914
EarI CTCTTC 2 cut(s) 548, 1914
Eco130I CCWWGG 1 cut(s) 1054
Eco32I GATATC 1 cut(s) 1195
Eco47I GGWCC 3 cut(s) 1014, 1276, 1574
Eco57I CTGAAG 4 cut(s) 1284, 1676, 1896, 2197
Eco81I CCTNAGG 2 cut(s) 74, 2430
Eco88I CYCGRG 3 cut(s) 796, 1003, 1977
EcoNI CCTNNNNNAGG 2 cut(s) 73, 573
EcoO109I RGGNCCY 1 cut(s) 1276
EcoRI GAATTC 3 cut(s) 358, 1253, 1760
EcoRII CCWGG 6 cut(s) 184, 574, 724, 1380, 1870, 2503
EcoRV GATATC 1 cut(s) 1195
EcoT14I CCWWGG 1 cut(s) 1054
EcoT22I ATGCAT 1 cut(s) 2308
ErhI CCWWGG 1 cut(s) 1054
Esp3I CGTCTC 1 cut(s) 1053
FalI AAGNNNNNCTT 2 cut(s) 1458, 1490
FaqI GGGAC 3 cut(s) 527, 1938, 1995
FbaI TGATCA 1 cut(s) 2113
FblI GTMKAC 1 cut(s) 759
Fnu4HI GCNGC 6 cut(s) 90, 1051, 1216, 1296, 1546, 2501
FokI GGATG 6 cut(s) 1303, 1830, 2219, 2259, 2323, 2332
Fsp4HI GCNGC 6 cut(s) 90, 1051, 1216, 1296, 1546, 2501
GluI GCNGC 6 cut(s) 90, 1051, 1216, 1296, 1546, 2501
GsuI CTGGAG 1 cut(s) 558
HaeIII GGCC 3 cut(s) 201, 1046, 2126
HapII CCGG 5 cut(s) 202, 285, 494, 1017, 2276
HgaI GACGC 1 cut(s) 2540
HindIII AAGCTT 2 cut(s) 503, 1777
HpaII CCGG 5 cut(s) 202, 285, 494, 1017, 2276
HphI GGTGA 5 cut(s) 287, 1842, 1988, 2273, 2529
Hpy166II GTNNAC 5 cut(s) 222, 760, 837, 847, 1582
Hpy8I GTNNAC 5 cut(s) 222, 760, 837, 847, 1582
HpyAV CCTTC 9 cut(s) 79, 190, 961, 1433, 1556, 1627, 1857, 2303, 2499
HpyCH4III ACNGT 6 cut(s) 8, 226, 241, 373, 834, 2111
HpyCH4IV ACGT 1 cut(s) 1269
HpyF10VI GCNNNNNNNGC 2 cut(s) 304, 2320
HpyF3I CTNAG 9 cut(s) 74, 299, 334, 517, 639, 1430, 1780, 1947, 2430
HpySE526I ACGT 1 cut(s) 1269
Kpn2I TCCGGA 1 cut(s) 2275
Ksp22I TGATCA 1 cut(s) 2113
Kzo9I GATC 9 cut(s) 78, 692, 889, 1309, 1835, 2029, 2113, 2278, 2343
LguI GCTCTTC 1 cut(s) 548
LmnI GCTCC 4 cut(s) 577, 635, 780, 952
Lsp1109I GCAGC 6 cut(s) 101, 1062, 1202, 1307, 1557, 2512
LweI GCATC 4 cut(s) 831, 1705, 2301, 2310
MaeII ACGT 1 cut(s) 1269
MaeIII GTNAC 6 cut(s) 188, 479, 622, 1265, 1491, 1882
MalI GATC 9 cut(s) 80, 694, 891, 1311, 1837, 2031, 2115, 2280, 2345
MboI GATC 9 cut(s) 78, 692, 889, 1309, 1835, 2029, 2113, 2278, 2343
MflI RGATCY 3 cut(s) 78, 692, 2029
MhlI GDGCHC 2 cut(s) 560, 1592
MlyI GAGTC 4 cut(s) 1030, 1452, 1997, 2347
MmeI TCCRAC 2 cut(s) 768, 1378
Mph1103I ATGCAT 1 cut(s) 2308
MroI TCCGGA 1 cut(s) 2275
MroXI GAANNNNTTC 1 cut(s) 1746
MseI TTAA 9 cut(s) 327, 1086, 1116, 1620, 1857, 1893, 1902, 2012, 2289
MslI CAYNNNNRTG 3 cut(s) 47, 842, 1595
MspI CCGG 5 cut(s) 202, 285, 494, 1017, 2276
MspR9I CCNGG 7 cut(s) 186, 286, 576, 726, 1382, 1872, 2505
Mva1269I GAATGC 1 cut(s) 1145
MvaI CCWGG 6 cut(s) 186, 576, 726, 1382, 1872, 2505
MwoI GCNNNNNNNGC 2 cut(s) 304, 2320
NciI CCSGG 1 cut(s) 286
NcoI CCATGG 1 cut(s) 1054
NdeI CATATG 1 cut(s) 922
NdeII GATC 9 cut(s) 78, 692, 889, 1309, 1835, 2029, 2113, 2278, 2343
NheI GCTAGC 2 cut(s) 521, 2039
NlaIV GGNNCC 2 cut(s) 776, 2149
NmuCI GTSAC 3 cut(s) 479, 1265, 1882
NsiI ATGCAT 1 cut(s) 2308
NspI RCATGY 5 cut(s) 963, 1826, 1900, 2306, 2449
NspV TTCGAA 1 cut(s) 1030
OliI CACNNNNGTG 1 cut(s) 842
PaeI GCATGC 1 cut(s) 963
PaeR7I CTCGAG 1 cut(s) 796
PaqCI CACCTGC 1 cut(s) 143
PciI ACATGT 1 cut(s) 1822
PciSI GCTCTTC 1 cut(s) 548
PcsI WCGNNNNNNNCGW 2 cut(s) 598, 794
PctI GAATGC 1 cut(s) 1145
PdmI GAANNNNTTC 1 cut(s) 1746
PfeI GAWTC 9 cut(s) 278, 379, 1637, 1702, 1747, 1804, 1875, 2363, 2534
PflMI CCANNNNNTGG 1 cut(s) 725
PfoI TCCNGGA 1 cut(s) 574
PkrI GCNGC 6 cut(s) 91, 1052, 1217, 1297, 1547, 2502
PleI GAGTC 4 cut(s) 1030, 1451, 1997, 2347
PpsI GAGTC 4 cut(s) 1030, 1451, 1997, 2347
PpuMI RGGWCCY 1 cut(s) 1276
PscI ACATGT 1 cut(s) 1822
Psp5II RGGWCCY 1 cut(s) 1276
Psp6I CCWGG 6 cut(s) 184, 574, 724, 1380, 1870, 2503
PspGI CCWGG 6 cut(s) 184, 574, 724, 1380, 1870, 2503
PspN4I GGNNCC 2 cut(s) 776, 2149
PspPI GGNCC 4 cut(s) 199, 1014, 1276, 1574
PspPPI RGGWCCY 1 cut(s) 1276
PsrI GAACNNNNNNTAC 2 cut(s) 992, 1024
PstI CTGCAG 2 cut(s) 91, 1052
PstNI CAGNNNCTG 1 cut(s) 230
PsuI RGATCY 3 cut(s) 78, 692, 2029
RseI CAYNNNNRTG 3 cut(s) 47, 842, 1595
SapI GCTCTTC 1 cut(s) 548
SaqAI TTAA 9 cut(s) 327, 1086, 1116, 1620, 1857, 1893, 1902, 2012, 2289
SatI GCNGC 6 cut(s) 90, 1051, 1216, 1296, 1546, 2501
Sau3AI GATC 9 cut(s) 78, 692, 889, 1309, 1835, 2029, 2113, 2278, 2343
Sau96I GGNCC 4 cut(s) 199, 1014, 1276, 1574
ScaI AGTACT 1 cut(s) 315
SchI GAGTC 4 cut(s) 1030, 1452, 1997, 2347
ScrFI CCNGG 7 cut(s) 186, 286, 576, 726, 1382, 1872, 2505
SduI GDGCHC 2 cut(s) 560, 1592
SfaNI GCATC 4 cut(s) 831, 1705, 2301, 2310
SfcI CTRYAG 3 cut(s) 87, 1048, 2178
Sfr274I CTCGAG 1 cut(s) 796
SfuI TTCGAA 1 cut(s) 1030
SinI GGWCC 3 cut(s) 1014, 1276, 1574
SlaI CTCGAG 1 cut(s) 796
SmiMI CAYNNNNRTG 3 cut(s) 47, 842, 1595
SmlI CTYRAG 4 cut(s) 34, 796, 1133, 1731
SmoI CTYRAG 4 cut(s) 34, 796, 1133, 1731
SpeI ACTAGT 1 cut(s) 316
SphI GCATGC 1 cut(s) 963
SspI AATATT 2 cut(s) 2047, 2103
StyD4I CCNGG 7 cut(s) 184, 284, 574, 724, 1380, 1870, 2503
StyI CCWWGG 1 cut(s) 1054
TaaI ACNGT 6 cut(s) 8, 226, 241, 373, 834, 2111
TaiI ACGT 1 cut(s) 1272
TaqI TCGA 8 cut(s) 194, 356, 797, 1030, 1631, 2026, 2106, 2346
TatI WGTACW 6 cut(s) 3, 313, 768, 1410, 1581, 2129
TfiI GAWTC 9 cut(s) 278, 379, 1637, 1702, 1747, 1804, 1875, 2363, 2534
Tru1I TTAA 9 cut(s) 327, 1086, 1116, 1620, 1857, 1893, 1902, 2012, 2289
Tru9I TTAA 9 cut(s) 327, 1086, 1116, 1620, 1857, 1893, 1902, 2012, 2289
TscAI CASTG 4 cut(s) 899, 2116, 2479, 2521
TseFI GTSAC 3 cut(s) 479, 1265, 1882
TseI GCWGC 6 cut(s) 89, 1050, 1215, 1295, 1545, 2500
Tsp45I GTSAC 3 cut(s) 479, 1265, 1882
TspGWI ACGGA 5 cut(s) 441, 607, 1029, 1303, 1432
TspRI CASTG 4 cut(s) 899, 2116, 2479, 2521
Van91I CCANNNNNTGG 1 cut(s) 725
VpaK11BI GGWCC 3 cut(s) 1014, 1276, 1574
XagI CCTNNNNNAGG 2 cut(s) 73, 573
XapI RAATTY 5 cut(s) 358, 734, 1253, 1760, 2187
XbaI TCTAGA 1 cut(s) 2006
XceI RCATGY 5 cut(s) 963, 1826, 1900, 2306, 2449
XcmI CCANNNNNNNNNTGG 1 cut(s) 1964
XhoI CTCGAG 1 cut(s) 796
XmiI GTMKAC 1 cut(s) 759
XmnI GAANNNNTTC 1 cut(s) 1746
ZrmI AGTACT 1 cut(s) 315
Zsp2I ATGCAT 1 cut(s) 2308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.