MD05G1216300.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000207
Physical Location & Seq
Forward (+)
34729549 .. 34733202
3654 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1216300.v1.1.491

Sequence Viewer

Length: 2475 bp
ATGCAAGCCTTGAGAAGCTTTTATATGTGCTTGTTCTTCTTATTCTCCCTCCTACGAATCTCAGCTGCAACTGCACTGGGCACAACAATCACTCCGAGTGGATATATAAGAGATGGCGAAACTCTGGTTTCAGTAGGTGGAAGCTATGAACTGGGATTCTTCAGCCCTGGTAAATCGAAAAGCCGGTACGTGGGAATATGGTACACTGATTCTACTGATGCAGTTGTGTGGGTAGCCAACAGAGAAACACCACTTGGTGATTCTTCAGGACTTTTGATGCTCACCGAGCAAGGAGTTCTAGTCCTCCTTGTCAATAGCTCAAACAAGATTGTGTGGTCATCGAATTCATCAAGAATCGCCGGGAATCCAGTATCGCAACTCTTGGATTCTGGAAATCTTGTGGTGCAAGATGGGAACGAAACTAACCCGGATAACTTCTTGTGGCAGAGTTTTGATTATCCTTGTGACACATACCTACCGGAAATGAAGCTTGGTTGGGACTTGGTTACCGGTTTAGAGAGGTATCTCTCGTCTTGGAAGAGCACAGAAGATCCTGCTCCAGGAGAGTTTTCGGCACGAATTGATTATCGTGGCTTACCACAACTTTTTGTTATGAAAAGAGCTAAGAAAGTTGGTAGAGGAGGGTCATGGAATGGTCTTCATTTAACAGGATTTTCATCTGGACAACCAAACCCAATATCTAGGATTGAATTTGTGTGGAACAAGGATGAAGTCTACTGTCAGTTTAGGCTCCTAAACAGGTCGGTGTTCCAGAGATTAGTATTGAGCCCATTAGGCGTTTCGCTGCTGTTCACATGGGAGTATCAAACACATAGTTGGGAATTTGCCTCTACACTCCAAGAAGATCGGTGTGAAAGTTACGCCTTGTGTGGTGCATATACTATTTGCAAAATCAATAACTCTCCCATATGTGTATGCCTGAAGGGATTTGTACCAAAATCTCCACAAGATTGGAATTCGGGAAATTGGTCTGATGGGTGTGTTCGTATGACTCCATTGGCCTGCAGCCATGGTGATGGCTTCCTAACGTACAGTGGGGTTAAATTGCCAGACACATCTTCGTCTTGGTATGATAAAAGCATGAGCCTCGAGCAATGCAAGGGATTATGTCTGGGAAACTGCTCATGTACGGCGTATACAAATCTAGATGTCAGGGAGGGAGGAACTGGCTGCTTGCTTTGGTTTGATAACGTCATTGACATAAGAGAATTCACTCTCGGTCAGGATCTCTATATACGGATGGCAGCATCAGAACTAGATATTTCAGATCATGTTAAGAAAAAGAGCAATTTCAACAAGAGACAGCTACTTGCAATTCTCCTCAGCTCTGCAGTTTTTCTCATGGGAATGCTAATAGTTGGAATAATATTGTACATCCGGAAGAAGAAACTCAAAAATGAAGGACTCAGAACAAAGGATTGCGGAGAAGAGTACCCTGGAGAAGACGGGGAAGACATGGAGTTACCACTGTTTGACTTGACCACCATAGCTAAAGCCACCAACGGCTTTTCAAACTGCAACAAGCTGGGAGAAGGTGGCTTTGGACCTGTGTACAAGGGTACACTGATAGGAGGAAAAGAAATTGCAGTAAAGAGGCTTTCAAAGAATTCCGGACAAGGGACGAGAGAGTTCAAAACTGAAGTTATACTTATAGCCAGACTTCAGCATCGCAATCTTGTTAAGCTTCTCGGTTGTTGCATTCAAAAAGACGAAAAGATTCTAATATATGAATTCATGCCCAACAAAAGCTTGGACTTCTATATTTTTGATCAGGAGGGACAAAAATTGCTCAACTGGCCTAAGTGCTTCCACATTATCGTTGGAGTTGCCCGAGGGCTTCTTTATCTTCACCAAGACTCTAGATTAAGGATTATTCATCGAGATCTGAAACCTAGCAATATTCTGCTAGATAATAATATGAACCCGAAGATTTCAGACTTTGGCCTGGCTAAAACATTCGGCGGCGATCAAAGTCAGGCCAACACAAACAGAGTGGTTGGAACCTATGGTTATATGTCTTCGGAATATGCAGCAGATGGAATCTTCTCGATGAAATCTGATGTGTTTAGCTTCGGAGTCATATTGCTCGAGATGTTGAGTAGGAAGAAGAACAGGGGATTTCGTCATCCGGATCACGACCACAACCTTCTTGGACATGCATGGATACGATGGATTCAAGATAAACCACTAGAACTGATCGACAAAACGTTTTGTGATTCATGCAACATATCTGAAGTGGTAAGGTGTCTTCATGTGGGGCTGTTATGTGTGCAAAGAGTACCTGAGAATAGACCAAGCATGTCATCTGTGGTTCTGATGTTGAGCAGTGATGTTGCCTTGCCACCTCCAAAGCAGCCTGGTTTCTACACTGAACGAAGCGTCAATGAATCACCATCAAAGACACTTCTATGTTCAGAAAATACTTTCAGCACTACATGGATAGAACCTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

825

Amino Acids

92.37

Weight (kDa)

7.67

Isoelectric Point (pI)

42.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 75 - 180 1.6e-36 D-mannose binding lectin
S_locus_glycop PF00954 213 - 321 3.2e-23 S-locus glycoprotein domain
PAN_2 PF08276 342 - 408 2.1e-20 PAN-like domain
Pkinase PF00069 512 - 777 5.6e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 514 - 778 1.1e-43 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 782 - 824 1.4e-07 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27300
fragaria_vesca FvH4_3g15930 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g21350 FvH4_3g21400 FvH4_3g21400 FvH4_6g12332
malus_domestica MD02G1167100.v1.1 MD02G1167200.v1.1 MD03G1185600.v1.1 MD05G1213900.v1.1 MD05G1214100.v1.1 MD05G1214200.v1.1 MD05G1214700.v1.1 MD05G1216300.v1.1 MD05G1216800.v1.1 MD05G1217300.v1.1 MD05G1218000.v1.1 MD11G1231100.v1.1 MD11G1231200.v1.1 MD11G1231400.v1.1 MD11G1231500.v1.1 MD11G1232400.v1.1 MD11G1232500.v1.1
prunus_persica Prupe.4G142300_v2.0.a1 Prupe.4G142400_v2.0.a1 Prupe.4G142500_v2.0.a1 Prupe.4G142600_v2.0.a1 Prupe.4G142800_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195100_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195500_v2.0.a1 Prupe.4G195700_v2.0.a1 Prupe.4G195900_v2.0.a1
pyrus_communis pycom03g16540 pycom05g19920 pycom11g20460 pycom11g20480 pycom11g20490 pycom11g20500 pycom11g20530
rosa_chinensis RchiOBHm_Chr2g0139311 RchiOBHm_Chr2g0139351 RchiOBHm_Chr5g0026801 RchiOBHm_Chr5g0026851 RchiOBHm_Chr5g0026891 RchiOBHm_Chr5g0026911 RchiOBHm_Chr5g0036571 RchiOBHm_Chr5g0036591 RchiOBHm_Chr5g0036721 RchiOBHm_Chr5g0036741 RchiOBHm_Chr7g0222981
rosa_laevigata RLG00000002006 RLG00000019807 RLG00000032964 RLG00000032968 RLG00000032969 RLG00000033706 RLG00000033708 RLG00000033712 RLG00000033716
rosa_multiflora Rmu_co8256651.1_g000001 Rmu_co8279377.1_g000001 Rmu_co8429919.1_g000001 Rmu_co8490103.1_g000001 Rmu_sc0000774.1_g000002 Rmu_sc0002525.1_g000012 Rmu_sc0004223.1_g000008 Rmu_sc0004390.1_g000002 Rmu_sc0006059.1_g000014 Rmu_sc0006059.1_g000024 Rmu_sc0006514.1_g000020 Rmu_sc0014333.1_g000002 Rmu_ssc0000172.1_g000018 Rmu_ssc0000172.1_g000029
rosa_roxburghii Rroxscaffold_1G00044230 Rroxscaffold_1G00044260 Rroxscaffold_1G00044320 Rroxscaffold_1G00044340 Rroxscaffold_1G00052560 Rroxscaffold_1G00052570 Rroxscaffold_1G00052660 Rroxscaffold_1G00052720 Rroxscaffold_2G00105450 Rroxscaffold_2G00105460
rosa_rugosa Rorug02G0356900 Rorug02G0357000 Rorug02G0357100 Rorug02G0357200 Rorug02G0357300 Rorug02G0357400 Rorug02G0357500 Rorug02G0357600 Rorug05G0095400 Rorug05G0095500 Rorug05G0095500 Rorug05G0095600 Rorug05G0095700 Rorug05G0095800 Rorug05G0157700 Rorug05G0157800
rosa_samantha Rh2AG406800 Rh2AG406900 Rh2BG417300 Rh2CG392900 Rh2DG426200 Rh5AG189300 Rh5AG249400 Rh5AG250100 Rh5BG186300 Rh5BG186900 Rh5BG187000 Rh5BG249500 Rh5BG250600 Rh5BG250900 Rh5BG251500 Rh5BG251800 Rh5CG205500 Rh5CG206300 Rh5CG206700 Rh5CG283000 Rh5CG283300 Rh5CG283500 Rh5DG188500 Rh5DG188700 Rh5DG258700 Rh5DG258900 Rh5DG259600 Rh5DG259900 Rh7CG375800 Rh7DG352500
rosa_wichuraiana Rw2G032960 Rw2G032970 Rw2G033220 Rw2G033230 Rw5G017130 Rw5G017230 Rw5G022940 Rw5G022960 Rw5G022980 Rw7G030420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 735, 1157
AccIII TCCGGA 3 cut(s) 1398, 1631, 2149
AciI CCGC 2 cut(s) 1443, 1983
AclI AACGTT 1 cut(s) 2228
AclWI GGATC 3 cut(s) 545, 1254, 2160
AcsI RAATTY 7 cut(s) 343, 710, 842, 976, 1229, 1627, 1751
AcuI CTGAAG 6 cut(s) 145, 249, 962, 1667, 1680, 2274
AdeI CACNNNGTG 1 cut(s) 257
AfiI CCNNNNNNNGG 3 cut(s) 190, 560, 1638
AgeI ACCGGT 1 cut(s) 509
AgsI TTSAA 7 cut(s) 710, 1315, 1533, 1623, 1654, 1724, 2198
AjnI CCWGG 5 cut(s) 166, 559, 1456, 1965, 2377
AjuI GAANNNNNNNTTGG 6 cut(s) 237, 269, 474, 506, 1545, 1577
Alw21I GWGCWC 1 cut(s) 545
Alw26I GTCTC 1 cut(s) 1315
AlwI GGATC 3 cut(s) 545, 1254, 2160
Ama87I CYCGRG 3 cut(s) 1109, 1851, 2108
Aor13HI TCCGGA 3 cut(s) 1398, 1631, 2149
AoxI GGCC 4 cut(s) 1020, 1817, 1963, 1998
ApeKI GCWGC 7 cut(s) 65, 805, 1026, 1191, 1265, 2051, 2374
ApoI RAATTY 7 cut(s) 343, 710, 842, 976, 1229, 1627, 1751
AsiGI ACCGGT 1 cut(s) 509
Asp700I GAANNNNTTC 1 cut(s) 1737
AspS9I GGNCC 1 cut(s) 1565
AsuC2I CCSGG 2 cut(s) 361, 428
AsuHPI GGTGA 5 cut(s) 269, 274, 1046, 1862, 2403
AvaI CYCGRG 3 cut(s) 1109, 1851, 2108
AvaII GGWCC 1 cut(s) 1565
BaeGI GKGCMC 1 cut(s) 83
BanII GRGCYC 1 cut(s) 791
BarI GAAGNNNNNNTAC 2 cut(s) 2253, 2285
BbsI GAAGAC 5 cut(s) 650, 1470, 1479, 2031, 2261
Bbv12I GWGCWC 1 cut(s) 545
BbvCI CCTCAGC 1 cut(s) 1343
BbvI GCAGC 7 cut(s) 52, 792, 1038, 1178, 1277, 2063, 2386
BccI CCATC 8 cut(s) 107, 404, 989, 1031, 1255, 2051, 2184, 2422
BceAI ACGGC 2 cut(s) 1167, 1540
BcgI CGANNNNNNTGC 2 cut(s) 1090, 1124
BciT130I CCWGG 5 cut(s) 168, 561, 1458, 1967, 2379
BciVI GTATCC 1 cut(s) 2178
BclI TGATCA 1 cut(s) 1789
BcnI CCSGG 2 cut(s) 361, 428
BcoDI GTCTC 1 cut(s) 1315
BfaI CTAG 8 cut(s) 299, 702, 1166, 1277, 1881, 1914, 1928, 2210
BfmI CTRYAG 2 cut(s) 1024, 1350
BfuI GTATCC 1 cut(s) 2178
BglI GCCNNNNNGGC 1 cut(s) 795
BglII AGATCT 1 cut(s) 1903
BisI GCNGC 8 cut(s) 66, 806, 1027, 1192, 1266, 1984, 2052, 2375
BlsI GCNGC 8 cut(s) 67, 807, 1028, 1193, 1267, 1985, 2053, 2376
Bme1390I CCNGG 7 cut(s) 168, 361, 428, 561, 1458, 1967, 2379
Bme18I GGWCC 1 cut(s) 1565
BmeT110I CYCGRG 3 cut(s) 1109, 1851, 2108
BmgT120I GGNCC 1 cut(s) 1565
BmiI GGNNCC 2 cut(s) 752, 2023
BmrFI CCNGG 7 cut(s) 168, 361, 428, 561, 1458, 1967, 2379
BmrI ACTGGG 2 cut(s) 86, 161
BmsI GCATC 4 cut(s) 208, 267, 1277, 1696
BmuI ACTGGG 2 cut(s) 86, 161
BpiI GAAGAC 5 cut(s) 650, 1470, 1479, 2031, 2261
BpmI CTGGAG 2 cut(s) 543, 1479
Bpu10I CCTNAGC 1 cut(s) 1343
BpuEI CTTGAG 1 cut(s) 31
BpuMI CCSGG 2 cut(s) 361, 428
BsaAI YACGTR 1 cut(s) 190
BsaBI GATNNNNATC 1 cut(s) 676
BsaJI CCNNGG 5 cut(s) 166, 1030, 1456, 1852, 2468
BsaWI WCCGGW 5 cut(s) 478, 509, 1398, 1631, 2149
BsaXI ACNNNNNCTCC 4 cut(s) 76, 106, 285, 315
Bsc4I CCNNNNNNNGG 3 cut(s) 190, 560, 1638
Bse118I RCCGGY 2 cut(s) 183, 509
Bse1I ACTGG 5 cut(s) 81, 156, 368, 1192, 1820
Bse3DI GCAATG 1 cut(s) 1121
Bse8I GATNNNNATC 1 cut(s) 676
BseAI TCCGGA 3 cut(s) 1398, 1631, 2149
BseBI CCWGG 5 cut(s) 168, 561, 1458, 1967, 2379
BseDI CCNNGG 5 cut(s) 166, 1030, 1456, 1852, 2468
BseGI GGATG 4 cut(s) 733, 1266, 1395, 2146
BseJI GATNNNNATC 1 cut(s) 676
BseLI CCNNNNNNNGG 3 cut(s) 190, 560, 1638
BseMI GCAATG 1 cut(s) 1121
BseMII CTCAG 4 cut(s) 75, 1357, 1441, 2295
BseNI ACTGG 5 cut(s) 81, 156, 368, 1192, 1820
BseRI GAGGAG 2 cut(s) 654, 1331
BseSI GKGCMC 1 cut(s) 83
BseXI GCAGC 7 cut(s) 52, 792, 1038, 1178, 1277, 2063, 2386
BseYI CCCAGC 1 cut(s) 1546
BsgI GTGCAG 1 cut(s) 57
BshFI GGCC 4 cut(s) 1022, 1819, 1965, 2000
BshTI ACCGGT 1 cut(s) 509
BsiHKAI GWGCWC 1 cut(s) 545
BsiHKCI CYCGRG 3 cut(s) 1109, 1851, 2108
BsiSI CCGG 8 cut(s) 184, 360, 428, 479, 510, 1399, 1632, 2150
BslFI GGGAC 3 cut(s) 512, 1654, 1812
BslI CCNNNNNNNGG 3 cut(s) 190, 560, 1638
BsmAI GTCTC 1 cut(s) 1315
BsmFI GGGAC 3 cut(s) 512, 1654, 1812
BsmI GAATGC 2 cut(s) 1374, 1719
BsnI GGCC 4 cut(s) 1022, 1819, 1965, 2000
BsoBI CYCGRG 3 cut(s) 1109, 1851, 2108
Bsp1286I GDGCHC 3 cut(s) 83, 545, 791
Bsp13I TCCGGA 3 cut(s) 1398, 1631, 2149
Bsp1407I TGTACA 2 cut(s) 1392, 1572
Bsp143I GATC 9 cut(s) 550, 865, 1246, 1288, 1789, 1903, 1987, 2152, 2217
Bsp19I CCATGG 1 cut(s) 1030
BspACI CCGC 2 cut(s) 1443, 1983
BspANI GGCC 4 cut(s) 1022, 1819, 1965, 2000
BspCNI CTCAG 4 cut(s) 74, 1356, 1440, 2296
BspEI TCCGGA 3 cut(s) 1398, 1631, 2149
BspLI GGNNCC 2 cut(s) 752, 2023
BspMAI CTGCAG 2 cut(s) 1028, 1354
BspPI GGATC 3 cut(s) 545, 1254, 2160
BspQI GCTCTTC 1 cut(s) 533
BsrDI GCAATG 1 cut(s) 1121
BsrFI RCCGGY 2 cut(s) 183, 509
BsrGI TGTACA 2 cut(s) 1392, 1572
BsrI ACTGG 5 cut(s) 81, 156, 368, 1192, 1820
BssAI RCCGGY 2 cut(s) 183, 509
BssECI CCNNGG 5 cut(s) 166, 1030, 1456, 1852, 2468
BssMI GATC 9 cut(s) 550, 865, 1246, 1288, 1789, 1903, 1987, 2152, 2217
BssNAI GTATAC 1 cut(s) 1158
BssT1I CCWWGG 1 cut(s) 1030
Bst1107I GTATAC 1 cut(s) 1158
Bst2UI CCWGG 5 cut(s) 168, 561, 1458, 1967, 2379
Bst4CI ACNGT 3 cut(s) 740, 1055, 1491
Bst6I CTCTTC 2 cut(s) 533, 1443
BstAUI TGTACA 2 cut(s) 1392, 1572
BstBAI YACGTR 1 cut(s) 190
BstC8I GCNNGC 3 cut(s) 6, 1024, 1196
BstDEI CTNAG 6 cut(s) 61, 624, 1343, 1427, 1821, 2304
BstDSI CCRYGG 1 cut(s) 1030
BstEII GGTNACC 1 cut(s) 505
BstENI CCTNNNNNAGG 1 cut(s) 558
BstF5I GGATG 4 cut(s) 733, 1266, 1395, 2146
BstKTI GATC 9 cut(s) 553, 868, 1249, 1291, 1792, 1906, 1990, 2155, 2220
BstMAI GTCTC 1 cut(s) 1315
BstMBI GATC 9 cut(s) 550, 865, 1246, 1288, 1789, 1903, 1987, 2152, 2217
BstMWI GCNNNNNNNGC 4 cut(s) 71, 286, 795, 1816
BstNI CCWGG 5 cut(s) 168, 561, 1458, 1967, 2379
BstNSI RCATGY 2 cut(s) 2180, 2323
BstPI GGTNACC 1 cut(s) 505
BstSCI CCNGG 7 cut(s) 166, 359, 426, 559, 1456, 1965, 2377
BstSFI CTRYAG 2 cut(s) 1024, 1350
BstSLI GKGCMC 1 cut(s) 83
BstV1I GCAGC 7 cut(s) 52, 792, 1038, 1178, 1277, 2063, 2386
BstV2I GAAGAC 5 cut(s) 650, 1470, 1479, 2031, 2261
BstX2I RGATCY 3 cut(s) 550, 1246, 1903
BstXI CCANNNNNNTGG 2 cut(s) 972, 1037
BstYI RGATCY 3 cut(s) 550, 1246, 1903
BstZ17I GTATAC 1 cut(s) 1158
BsuI GTATCC 1 cut(s) 2178
BsuRI GGCC 4 cut(s) 1022, 1819, 1965, 2000
BtgI CCRYGG 1 cut(s) 1030
BtgZI GCGATG 1 cut(s) 1673
BtsCI GGATG 4 cut(s) 733, 1266, 1395, 2146
BtsI GCAGTG 1 cut(s) 2353
BtsIMutI CAGTG 7 cut(s) 74, 204, 1060, 1487, 1583, 2353, 2388
Cac8I GCNNGC 3 cut(s) 6, 1024, 1196
Cfr10I RCCGGY 2 cut(s) 183, 509
Cfr13I GGNCC 1 cut(s) 1565
CseI GACGC 1 cut(s) 2389
CspAI ACCGGT 1 cut(s) 509
CspCI CAANNNNNGTGG 2 cut(s) 1995, 2030
DdeI CTNAG 6 cut(s) 61, 624, 1343, 1427, 1821, 2304
DpnI GATC 9 cut(s) 552, 867, 1248, 1290, 1791, 1905, 1989, 2154, 2219
DpnII GATC 9 cut(s) 550, 865, 1246, 1288, 1789, 1903, 1987, 2152, 2217
DraIII CACNNNGTG 1 cut(s) 257
Eam1104I CTCTTC 2 cut(s) 533, 1443
EarI CTCTTC 2 cut(s) 533, 1443
Eco130I CCWWGG 1 cut(s) 1030
Eco24I GRGCYC 1 cut(s) 791
Eco47I GGWCC 1 cut(s) 1565
Eco57I CTGAAG 6 cut(s) 145, 249, 962, 1667, 1680, 2274
Eco88I CYCGRG 3 cut(s) 1109, 1851, 2108
Eco91I GGTNACC 1 cut(s) 505
EcoNI CCTNNNNNAGG 1 cut(s) 558
EcoO65I GGTNACC 1 cut(s) 505
EcoRI GAATTC 5 cut(s) 343, 976, 1229, 1627, 1751
EcoRII CCWGG 5 cut(s) 166, 559, 1456, 1965, 2377
EcoT14I CCWWGG 1 cut(s) 1030
EcoT22I ATGCAT 1 cut(s) 2182
EcoT38I GRGCYC 1 cut(s) 791
ErhI CCWWGG 1 cut(s) 1030
FalI AAGNNNNNCTT 2 cut(s) 1653, 1685
FaqI GGGAC 3 cut(s) 512, 1654, 1812
FauNDI CATATG 1 cut(s) 929
FbaI TGATCA 1 cut(s) 1789
FblI GTMKAC 2 cut(s) 735, 1157
Fnu4HI GCNGC 8 cut(s) 66, 806, 1027, 1192, 1266, 1984, 2052, 2375
FokI GGATG 4 cut(s) 740, 1273, 1382, 2133
FriOI GRGCYC 1 cut(s) 791
Fsp4HI GCNGC 8 cut(s) 66, 806, 1027, 1192, 1266, 1984, 2052, 2375
FspBI CTAG 8 cut(s) 299, 702, 1166, 1277, 1881, 1914, 1928, 2210
GluI GCNGC 8 cut(s) 66, 806, 1027, 1192, 1266, 1984, 2052, 2375
GsaI CCCAGC 1 cut(s) 1550
GsuI CTGGAG 2 cut(s) 543, 1479
HaeIII GGCC 4 cut(s) 1022, 1819, 1965, 2000
HapII CCGG 8 cut(s) 184, 360, 428, 479, 510, 1399, 1632, 2150
HgaI GACGC 1 cut(s) 2389
HindIII AAGCTT 4 cut(s) 16, 488, 1703, 1768
HpaII CCGG 8 cut(s) 184, 360, 428, 479, 510, 1399, 1632, 2150
HphI GGTGA 5 cut(s) 269, 274, 1046, 1862, 2403
Hpy166II GTNNAC 6 cut(s) 204, 736, 813, 1158, 1573, 1583
Hpy8I GTNNAC 6 cut(s) 204, 736, 813, 1158, 1573, 1583
HpyAV CCTTC 4 cut(s) 937, 1415, 1547, 2177
HpyCH4III ACNGT 3 cut(s) 740, 1055, 1491
HpyCH4IV ACGT 4 cut(s) 189, 1049, 1212, 2228
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 286, 795, 1816
HpyF3I CTNAG 6 cut(s) 61, 624, 1343, 1427, 1821, 2304
HpySE526I ACGT 4 cut(s) 189, 1049, 1212, 2228
Kpn2I TCCGGA 3 cut(s) 1398, 1631, 2149
Ksp22I TGATCA 1 cut(s) 1789
Kzo9I GATC 9 cut(s) 550, 865, 1246, 1288, 1789, 1903, 1987, 2152, 2217
LguI GCTCTTC 1 cut(s) 533
LmnI GCTCC 2 cut(s) 562, 756
Lsp1109I GCAGC 7 cut(s) 52, 792, 1038, 1178, 1277, 2063, 2386
LweI GCATC 4 cut(s) 208, 267, 1277, 1696
MaeI CTAG 8 cut(s) 299, 702, 1166, 1277, 1881, 1914, 1928, 2210
MaeII ACGT 4 cut(s) 189, 1049, 1212, 2228
MaeIII GTNAC 4 cut(s) 464, 505, 878, 1482
MalI GATC 9 cut(s) 552, 867, 1248, 1290, 1791, 1905, 1989, 2154, 2219
MboI GATC 9 cut(s) 550, 865, 1246, 1288, 1789, 1903, 1987, 2152, 2217
MflI RGATCY 3 cut(s) 550, 1246, 1903
MhlI GDGCHC 3 cut(s) 83, 545, 791
MlyI GAGTC 4 cut(s) 1006, 1419, 1871, 2106
MmeI TCCRAC 3 cut(s) 1360, 1822, 1999
Mph1103I ATGCAT 1 cut(s) 2182
MroI TCCGGA 3 cut(s) 1398, 1631, 2149
MroXI GAANNNNTTC 1 cut(s) 1737
MseI TTAA 5 cut(s) 665, 1062, 1296, 1701, 1886
MslI CAYNNNNRTG 3 cut(s) 1035, 1367, 2428
MspA1I CMGCKG 1 cut(s) 65
MspI CCGG 8 cut(s) 184, 360, 428, 479, 510, 1399, 1632, 2150
MspR9I CCNGG 7 cut(s) 168, 361, 428, 561, 1458, 1967, 2379
Mva1269I GAATGC 2 cut(s) 1374, 1719
MvaI CCWGG 5 cut(s) 168, 561, 1458, 1967, 2379
MwoI GCNNNNNNNGC 4 cut(s) 71, 286, 795, 1816
NciI CCSGG 2 cut(s) 361, 428
NcoI CCATGG 1 cut(s) 1030
NdeI CATATG 1 cut(s) 929
NdeII GATC 9 cut(s) 550, 865, 1246, 1288, 1789, 1903, 1987, 2152, 2217
NlaIV GGNNCC 2 cut(s) 752, 2023
NmuCI GTSAC 1 cut(s) 464
NsiI ATGCAT 1 cut(s) 2182
NspI RCATGY 2 cut(s) 2180, 2323
PaeR7I CTCGAG 2 cut(s) 1109, 2108
PciSI GCTCTTC 1 cut(s) 533
PctI GAATGC 2 cut(s) 1374, 1719
PdmI GAANNNNTTC 1 cut(s) 1737
PfoI TCCNGGA 1 cut(s) 559
PinAI ACCGGT 1 cut(s) 509
PkrI GCNGC 8 cut(s) 67, 807, 1028, 1193, 1267, 1985, 2053, 2376
PleI GAGTC 4 cut(s) 1006, 1419, 1871, 2105
PpsI GAGTC 4 cut(s) 1006, 1419, 1871, 2105
Ppu21I YACGTR 1 cut(s) 190
Psp1406I AACGTT 1 cut(s) 2228
Psp6I CCWGG 5 cut(s) 166, 559, 1456, 1965, 2377
PspEI GGTNACC 1 cut(s) 505
PspFI CCCAGC 1 cut(s) 1546
PspGI CCWGG 5 cut(s) 166, 559, 1456, 1965, 2377
PspN4I GGNNCC 2 cut(s) 752, 2023
PspPI GGNCC 1 cut(s) 1565
PspXI VCTCGAGB 1 cut(s) 1109
PstI CTGCAG 2 cut(s) 1028, 1354
PsuI RGATCY 3 cut(s) 550, 1246, 1903
PvuII CAGCTG 1 cut(s) 65
RseI CAYNNNNRTG 3 cut(s) 1035, 1367, 2428
SapI GCTCTTC 1 cut(s) 533
SaqAI TTAA 5 cut(s) 665, 1062, 1296, 1701, 1886
SatI GCNGC 8 cut(s) 66, 806, 1027, 1192, 1266, 1984, 2052, 2375
Sau3AI GATC 9 cut(s) 550, 865, 1246, 1288, 1789, 1903, 1987, 2152, 2217
Sau96I GGNCC 1 cut(s) 1565
SchI GAGTC 4 cut(s) 1006, 1419, 1871, 2106
ScrFI CCNGG 7 cut(s) 168, 361, 428, 561, 1458, 1967, 2379
SduI GDGCHC 3 cut(s) 83, 545, 791
SfaNI GCATC 4 cut(s) 208, 267, 1277, 1696
SfcI CTRYAG 2 cut(s) 1024, 1350
Sfr274I CTCGAG 2 cut(s) 1109, 2108
SinI GGWCC 1 cut(s) 1565
SlaI CTCGAG 2 cut(s) 1109, 2108
SmiMI CAYNNNNRTG 3 cut(s) 1035, 1367, 2428
SmlI CTYRAG 3 cut(s) 10, 1109, 2108
SmoI CTYRAG 3 cut(s) 10, 1109, 2108
SsiI CCGC 2 cut(s) 1443, 1983
SspI AATATT 2 cut(s) 1389, 1921
SspMI CTAG 8 cut(s) 299, 702, 1166, 1277, 1881, 1914, 1928, 2210
StyD4I CCNGG 7 cut(s) 166, 359, 426, 559, 1456, 1965, 2377
StyI CCWWGG 1 cut(s) 1030
TaaI ACNGT 3 cut(s) 740, 1055, 1491
TaiI ACGT 4 cut(s) 192, 1052, 1215, 2231
TaqI TCGA 7 cut(s) 176, 341, 1110, 1900, 2069, 2109, 2220
TaqII GACCGA 1 cut(s) 1229
TatI WGTACW 2 cut(s) 1392, 1572
TauI GCSGC 1 cut(s) 1986
Tru1I TTAA 5 cut(s) 665, 1062, 1296, 1701, 1886
Tru9I TTAA 5 cut(s) 665, 1062, 1296, 1701, 1886
TscAI CASTG 7 cut(s) 81, 211, 1060, 1494, 1590, 2353, 2395
TseFI GTSAC 1 cut(s) 464
TseI GCWGC 7 cut(s) 65, 805, 1026, 1191, 1265, 2051, 2374
Tsp45I GTSAC 1 cut(s) 464
TspGWI ACGGA 1 cut(s) 1273
TspRI CASTG 7 cut(s) 81, 211, 1060, 1494, 1590, 2353, 2395
VpaK11BI GGWCC 1 cut(s) 1565
XagI CCTNNNNNAGG 1 cut(s) 558
XapI RAATTY 7 cut(s) 343, 710, 842, 976, 1229, 1627, 1751
XbaI TCTAGA 2 cut(s) 1165, 1880
XceI RCATGY 2 cut(s) 2180, 2323
XcmI CCANNNNNNNNNTGG 3 cut(s) 1768, 1838, 2168
XhoI CTCGAG 2 cut(s) 1109, 2108
XmiI GTMKAC 2 cut(s) 735, 1157
XmnI GAANNNNTTC 1 cut(s) 1737
XspI CTAG 8 cut(s) 299, 702, 1166, 1277, 1881, 1914, 1928, 2210
Zsp2I ATGCAT 1 cut(s) 2182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.