Rroxscaffold_1G00052660

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000207
Physical Location & Seq
Forward (+)
73419863 .. 73423441
3579 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00052660.1

Sequence Viewer

Length: 2370 bp
ATGCAAGCCTTTAGAACCCTTTTTTTGTGCTACTTCTTTTTCTCCTCCGTAAAAACCCCATTTACAACTGCACTAGACGCTATAACTCCAAGTCAATATATTAGAGTTGGTGAAACTCTAGTTTCTGCTGATGGAAACTTTGAACTGGGATTCTTTGGTAAGTCGAAAGGCCAATACTTGGGAATATGGTACACTTTTTCTACTGACATAGTTGTGTGGGTAGCCAACAGAGAAACACCGGTTAATGATTCTTCAGGAGTCTTAAAGCTCACTGACCAGGGAGTTTTAGTCCTTCTCAATAGCTCAAATGGCACTGTATGGTCAACCAATTCATCAAGAACTGCAGGGAATCCAGTCTTGCAACTCTTGGATTCGGGAAATCTTGTTGTGAAAGATGGAAATGATACTAACCCTGATTACTTTCTTTGGCAGAGTTTTGATTATCCTTGTGACACACAACTACCAGGAATGAAACTTGGCCGGAACTTAGTTAATGGTTTAGACAGGTATCTCTCGTCTTGGAGGAGCACAGATGATCCTGCTCAAGGAGAGCTTTCACTACGGATGGATCCTCGTGGTTTACCTCAATTTTTTGTAGTTCAGGGAGCCAAGATACTGACTAGAGCAGGATCATGGAACGGCCTTCAATTGACTGGATATTATCAAAGCAGGCCAAATCCAATATCTGAGTTTGTATTTGTGTCAAATGAGACAGAAATCTATTACGAGTACACACTCCTCAACCGGTCTACGTTCTCTAGATATGTAATGAATCCGTATGGCACTACACAGTGGTTAACATGGATAGACTACACAAATAGTTGGGAGCCTTTCTTTGCAAACCGAGCAGATCAGTGTGAAATTTACGCTTTTTGTGGGGCTAATGCTAATTGTAACGTCAATGAAGCCCCCATGTGTGCATGCTTGAAAGGATTTGTACCTAAATCTCCACAAAAGTGGAACTCTTCACGTTGGTCTGATGGATGTGTTCGTAGGACTCCATTAGTTTGCAGTGATACAGATGACTTCTTAATATATAGTAGATTTAAATTGCCAGACACATCTTCTTCCTGGTATCACAAGAGCATGAACCTCAAGGAATGCAAGGAATTGTGTTTGAGAAACTGCTCGTGTACGGCTTATGCAAATTTAGATGTCAGGGAAGGGGAAGTGGCTGCTTGCTTTGATGCTATTGGGAAGAAGAGCAAGTCCAACAAGAGAAGGCAAGCTGGAATTGTAATCAGCTCTGCTCTTCTTGGAGTGGGAATGCTAATACTTGGATTCCTCTTTTATAAACGGAACAAGAAACTCAGAAATCAAGATGTTAGAAGATTGCTGGATTGCCGAATGGATGACTGTGAAGAAGGAAGGGAAGACATGGAGTTACCGCTATTTGACTTTACCACTATAGCTGATGCCACTGATAACTTTTCAAGCAACAACAAACAGGGACAAGGCGGTTTTGGACCTGTGTACAAGGGTACATTGATAGGAGGGGAAGAAATAGCTGTAAAGAGGGGATCCAAGGAATCTGGTCAAGGAATGAGGGAGTTCAAAAATGAAGTTCTACTGATAGCCAAACTTCAGCACCGTAATCTTGTAAAGCTTCTGGGCTGTTGCATTCAAAATGATGAAAAAATATTAATATATGAATACATGTCCAACAGGAGCTTGGACTTCTTTATATTTGACCAGGAAAGACAGAAATCGCTCGACTGGCCTACCTGCTACAATATTATTGATGGAATTGCACGAGGGCTTCTCTATCTTCACCAAGACTCGAGATTAAGAATTGTTCACAGAGATCTGAAACCTGGTAATATTTTGCTAGATGAAAACATGAACCCAAAGATTTCAGATTTTGGCCTAGCTAAAACATTTAGCTTTGATCAAAGTCAAGCCAATACGAATAAAGTGGCTGGAACATATGGTTATATGGCGCCTGAATATGCAGTAGATGGAATCTTCTCGGCGAAATCAGATGTATTTAGCTTTGGAGTTGTGCTGATTGAGTTATTGAGTAGGGAGAAGAACAGGGGATTTTGTCATCCAGATCACGATTTTAATCTTCTTGGACATGCATGGACGCTATGGACTCAAAATGTGCCACTGGAACTCATCGATAAGACATTATGTGATCCGGACACCACATCCGAAGTTTTAAGGTGTCTTCATGTAGGTCTGTTATGTGTGCAACAAGTACCTGAAGATAGACCAAGCATGTCATCTGTGGTTCTAATGTTGGGCAGTCATGATGTTGTGCTGCCTTTGCCAAAGCAGCCTGGATTTTACACTGAACGGACTCTTCCCGAATCACGTGACCTGTGTTCACCCAGTAACTTCAGCACTACATTGTTAGAGGGTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

789

Amino Acids

89.1

Weight (kDa)

5.88

Isoelectric Point (pI)

41.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 71 - 175 1.1e-37 D-mannose binding lectin
S_locus_glycop PF00954 208 - 316 6.2e-24 S-locus glycoprotein domain
PAN_2 PF08276 337 - 394 5.4e-15 PAN-like domain
Pkinase PF00069 479 - 744 1.5e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 481 - 745 2.1e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27300
fragaria_vesca FvH4_3g15930 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g21350 FvH4_3g21400 FvH4_3g21400 FvH4_6g12332
malus_domestica MD02G1167100.v1.1 MD02G1167200.v1.1 MD03G1185600.v1.1 MD05G1213900.v1.1 MD05G1214100.v1.1 MD05G1214200.v1.1 MD05G1214700.v1.1 MD05G1216300.v1.1 MD05G1216800.v1.1 MD05G1217300.v1.1 MD05G1218000.v1.1 MD11G1231100.v1.1 MD11G1231200.v1.1 MD11G1231400.v1.1 MD11G1231500.v1.1 MD11G1232400.v1.1 MD11G1232500.v1.1
prunus_persica Prupe.4G142300_v2.0.a1 Prupe.4G142400_v2.0.a1 Prupe.4G142500_v2.0.a1 Prupe.4G142600_v2.0.a1 Prupe.4G142800_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195100_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195500_v2.0.a1 Prupe.4G195700_v2.0.a1 Prupe.4G195900_v2.0.a1
pyrus_communis pycom03g16540 pycom05g19920 pycom11g20460 pycom11g20480 pycom11g20490 pycom11g20500 pycom11g20530
rosa_chinensis RchiOBHm_Chr2g0139311 RchiOBHm_Chr2g0139351 RchiOBHm_Chr5g0026801 RchiOBHm_Chr5g0026851 RchiOBHm_Chr5g0026891 RchiOBHm_Chr5g0026911 RchiOBHm_Chr5g0036571 RchiOBHm_Chr5g0036591 RchiOBHm_Chr5g0036721 RchiOBHm_Chr5g0036741 RchiOBHm_Chr7g0222981
rosa_laevigata RLG00000002006 RLG00000019807 RLG00000032964 RLG00000032968 RLG00000032969 RLG00000033706 RLG00000033708 RLG00000033712 RLG00000033716
rosa_multiflora Rmu_co8256651.1_g000001 Rmu_co8279377.1_g000001 Rmu_co8429919.1_g000001 Rmu_co8490103.1_g000001 Rmu_sc0000774.1_g000002 Rmu_sc0002525.1_g000012 Rmu_sc0004223.1_g000008 Rmu_sc0004390.1_g000002 Rmu_sc0006059.1_g000014 Rmu_sc0006059.1_g000024 Rmu_sc0006514.1_g000020 Rmu_sc0014333.1_g000002 Rmu_ssc0000172.1_g000018 Rmu_ssc0000172.1_g000029
rosa_roxburghii Rroxscaffold_1G00044230 Rroxscaffold_1G00044260 Rroxscaffold_1G00044320 Rroxscaffold_1G00044340 Rroxscaffold_1G00052560 Rroxscaffold_1G00052570 Rroxscaffold_1G00052660 Rroxscaffold_1G00052720 Rroxscaffold_2G00105450 Rroxscaffold_2G00105460
rosa_rugosa Rorug02G0356900 Rorug02G0357000 Rorug02G0357100 Rorug02G0357200 Rorug02G0357300 Rorug02G0357400 Rorug02G0357500 Rorug02G0357600 Rorug05G0095400 Rorug05G0095500 Rorug05G0095500 Rorug05G0095600 Rorug05G0095700 Rorug05G0095800 Rorug05G0157700 Rorug05G0157800
rosa_samantha Rh2AG406800 Rh2AG406900 Rh2BG417300 Rh2CG392900 Rh2DG426200 Rh5AG189300 Rh5AG249400 Rh5AG250100 Rh5BG186300 Rh5BG186900 Rh5BG187000 Rh5BG249500 Rh5BG250600 Rh5BG250900 Rh5BG251500 Rh5BG251800 Rh5CG205500 Rh5CG206300 Rh5CG206700 Rh5CG283000 Rh5CG283300 Rh5CG283500 Rh5DG188500 Rh5DG188700 Rh5DG258700 Rh5DG258900 Rh5DG259600 Rh5DG259900 Rh7CG375800 Rh7DG352500
rosa_wichuraiana Rw2G032960 Rw2G032970 Rw2G033220 Rw2G033230 Rw5G017130 Rw5G017230 Rw5G022940 Rw5G022960 Rw5G022980 Rw7G030420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1293
Acc36I ACCTGC 1 cut(s) 1733
AccB1I GGYRCC 1 cut(s) 1939
AccB7I CCANNNNNTGG 1 cut(s) 178
AccI GTMKAC 1 cut(s) 749
AccIII TCCGGA 1 cut(s) 2140
AciI CCGC 2 cut(s) 1388, 1458
AclWI GGATC 7 cut(s) 530, 563, 576, 637, 1515, 1528, 2132
AcoI YGGCCR 1 cut(s) 478
AcsI RAATTY 2 cut(s) 861, 1147
AcuI CTGAAG 4 cut(s) 237, 1568, 2226, 2326
AcvI CACGTG 1 cut(s) 2318
AcyI GRCGYC 1 cut(s) 1940
AfaI GTAC 7 cut(s) 191, 731, 939, 1135, 1475, 1483, 2202
AfiI CCNNNNNNNGG 2 cut(s) 178, 545
AflIII ACRYGT 1 cut(s) 1656
AgeI ACCGGT 2 cut(s) 238, 744
AgsI TTSAA 6 cut(s) 143, 647, 928, 1434, 1555, 1625
AjnI CCWGG 6 cut(s) 276, 463, 1070, 1692, 1813, 2281
AleI CACNNNNGTG 1 cut(s) 955
Alw21I GWGCWC 1 cut(s) 530
Alw26I GTCTC 1 cut(s) 704
AlwI GGATC 7 cut(s) 530, 563, 576, 637, 1515, 1528, 2132
Ama87I CYCGRG 1 cut(s) 1780
Aor13HI TCCGGA 1 cut(s) 2140
AoxI GGCC 6 cut(s) 169, 478, 640, 671, 1718, 1864
ApeKI GCWGC 3 cut(s) 1175, 2263, 2278
ApoI RAATTY 2 cut(s) 861, 1147
AseI ATTAAT 1 cut(s) 1643
AsiGI ACCGGT 2 cut(s) 238, 744
AspLEI GCGC 1 cut(s) 1942
AspS9I GGNCC 1 cut(s) 1466
AsuHPI GGTGA 3 cut(s) 122, 1763, 2322
AvaI CYCGRG 1 cut(s) 1780
AvaII GGWCC 1 cut(s) 1466
BamHI GGATCC 2 cut(s) 568, 1520
BanI GGYRCC 1 cut(s) 1939
BauI CACGAG 3 cut(s) 573, 1129, 1752
BbrPI CACGTG 1 cut(s) 2318
BbsI GAAGAC 2 cut(s) 1380, 2162
Bbv12I GWGCWC 1 cut(s) 530
BbvI GCAGC 3 cut(s) 1162, 2250, 2290
BccI CCATC 6 cut(s) 125, 389, 559, 974, 1736, 1952
BceAI ACGGC 2 cut(s) 655, 1152
BciT130I CCWGG 6 cut(s) 278, 465, 1072, 1694, 1815, 2283
BclI TGATCA 1 cut(s) 1888
BcoDI GTCTC 1 cut(s) 704
BfaI CTAG 6 cut(s) 74, 119, 621, 759, 1829, 1868
BfmI CTRYAG 2 cut(s) 342, 1407
BfoI RGCGCY 1 cut(s) 1943
BfuAI ACCTGC 1 cut(s) 1733
BglII AGATCT 1 cut(s) 1804
BisI GCNGC 3 cut(s) 1176, 2264, 2279
BlsI GCNGC 3 cut(s) 1177, 2265, 2280
Bme1390I CCNGG 6 cut(s) 278, 465, 1072, 1694, 1815, 2283
Bme18I GGWCC 1 cut(s) 1466
BmeT110I CYCGRG 1 cut(s) 1780
BmgT120I GGNCC 1 cut(s) 1466
BmiI GGNNCC 5 cut(s) 570, 607, 828, 1522, 1941
BmrFI CCNGG 6 cut(s) 278, 465, 1072, 1694, 1815, 2283
BmrI ACTGGG 2 cut(s) 155, 2328
BmsI GCATC 2 cut(s) 1177, 1405
BmuI ACTGGG 2 cut(s) 155, 2328
BpiI GAAGAC 2 cut(s) 1380, 2162
BplI GAGNNNNNCTC 2 cut(s) 1746, 1778
BpuEI CTTGAG 2 cut(s) 528, 1079
Bsa29I ATCGAT 1 cut(s) 2121
BsaAI YACGTR 1 cut(s) 2318
BsaBI GATNNNNATC 1 cut(s) 2064
BsaHI GRCGYC 1 cut(s) 1940
BsaJI CCNNGG 2 cut(s) 277, 1524
BsaWI WCCGGW 3 cut(s) 238, 744, 2140
BsaXI ACNNNNNCTCC 2 cut(s) 273, 303
Bsc4I CCNNNNNNNGG 2 cut(s) 178, 545
Bse118I RCCGGY 2 cut(s) 238, 744
Bse1I ACTGG 6 cut(s) 150, 353, 658, 1721, 2115, 2334
Bse8I GATNNNNATC 1 cut(s) 2064
BseAI TCCGGA 1 cut(s) 2140
BseBI CCWGG 6 cut(s) 278, 465, 1072, 1694, 1815, 2283
BseCI ATCGAT 1 cut(s) 2121
BseDI CCNNGG 2 cut(s) 277, 1524
BseGI GGATG 5 cut(s) 570, 989, 1357, 2047, 2150
BseJI GATNNNNATC 1 cut(s) 2064
BseLI CCNNNNNNNGG 2 cut(s) 178, 545
BseMII CTCAG 2 cut(s) 678, 1324
BseNI ACTGG 6 cut(s) 150, 353, 658, 1721, 2115, 2334
BseRI GAGGAG 3 cut(s) 34, 538, 728
BseXI GCAGC 3 cut(s) 1162, 2250, 2290
BsgI GTGCAG 1 cut(s) 54
BshFI GGCC 6 cut(s) 171, 480, 642, 673, 1720, 1866
BshNI GGYRCC 1 cut(s) 1939
BshTI ACCGGT 2 cut(s) 238, 744
BshVI ATCGAT 1 cut(s) 2121
BsiHKAI GWGCWC 1 cut(s) 530
BsiHKCI CYCGRG 1 cut(s) 1780
BsiSI CCGG 4 cut(s) 239, 481, 745, 2141
BslFI GGGAC 1 cut(s) 1464
BslI CCNNNNNNNGG 2 cut(s) 178, 545
BsmAI GTCTC 1 cut(s) 704
BsmFI GGGAC 1 cut(s) 1464
BsmI GAATGC 3 cut(s) 1106, 1272, 1620
BsnI GGCC 6 cut(s) 171, 480, 642, 673, 1720, 1866
BsoBI CYCGRG 1 cut(s) 1780
Bsp1286I GDGCHC 1 cut(s) 530
Bsp13I TCCGGA 1 cut(s) 2140
Bsp1407I TGTACA 1 cut(s) 1473
Bsp143I GATC 9 cut(s) 535, 568, 629, 850, 1520, 1804, 1888, 2053, 2137
BspACI CCGC 2 cut(s) 1388, 1458
BspANI GGCC 6 cut(s) 171, 480, 642, 673, 1720, 1866
BspCNI CTCAG 2 cut(s) 679, 1323
BspDI ATCGAT 1 cut(s) 2121
BspEI TCCGGA 1 cut(s) 2140
BspHI TCATGA 1 cut(s) 2251
BspLI GGNNCC 5 cut(s) 570, 607, 828, 1522, 1941
BspMAI CTGCAG 1 cut(s) 346
BspMI ACCTGC 1 cut(s) 1733
BspPI GGATC 7 cut(s) 530, 563, 576, 637, 1515, 1528, 2132
BspQI GCTCTTC 2 cut(s) 1196, 1257
BspT107I GGYRCC 1 cut(s) 1939
BsrFI RCCGGY 2 cut(s) 238, 744
BsrGI TGTACA 1 cut(s) 1473
BsrI ACTGG 6 cut(s) 150, 353, 658, 1721, 2115, 2334
BssAI RCCGGY 2 cut(s) 238, 744
BssECI CCNNGG 2 cut(s) 277, 1524
BssMI GATC 9 cut(s) 535, 568, 629, 850, 1520, 1804, 1888, 2053, 2137
BssNI GRCGYC 1 cut(s) 1940
BssSI CACGAG 3 cut(s) 573, 1129, 1752
BssT1I CCWWGG 1 cut(s) 1524
Bst2BI CACGAG 3 cut(s) 573, 1129, 1752
Bst2UI CCWGG 6 cut(s) 278, 465, 1072, 1694, 1815, 2283
Bst4CI ACNGT 4 cut(s) 316, 792, 1358, 1592
Bst6I CTCTTC 4 cut(s) 970, 1196, 1257, 2310
BstACI GRCGYC 1 cut(s) 1940
BstAUI TGTACA 1 cut(s) 1473
BstBAI YACGTR 1 cut(s) 2318
BstC8I GCNNGC 5 cut(s) 6, 671, 922, 1180, 1227
BstDEI CTNAG 3 cut(s) 487, 687, 1310
BstENI CCTNNNNNAGG 1 cut(s) 543
BstF5I GGATG 5 cut(s) 570, 989, 1357, 2047, 2150
BstH2I RGCGCY 1 cut(s) 1943
BstHHI GCGC 1 cut(s) 1942
BstKTI GATC 9 cut(s) 538, 571, 632, 853, 1523, 1807, 1891, 2056, 2140
BstMAI GTCTC 1 cut(s) 704
BstMBI GATC 9 cut(s) 535, 568, 629, 850, 1520, 1804, 1888, 2053, 2137
BstMWI GCNNNNNNNGC 6 cut(s) 77, 309, 845, 1717, 2269, 2278
BstNI CCWGG 6 cut(s) 278, 465, 1072, 1694, 1815, 2283
BstNSI RCATGY 4 cut(s) 924, 1660, 2081, 2224
BstSCI CCNGG 6 cut(s) 276, 463, 1070, 1692, 1813, 2281
BstSFI CTRYAG 2 cut(s) 342, 1407
BstV1I GCAGC 3 cut(s) 1162, 2250, 2290
BstV2I GAAGAC 2 cut(s) 1380, 2162
BstX2I RGATCY 3 cut(s) 568, 1520, 1804
BstXI CCANNNNNNTGG 1 cut(s) 957
BstYI RGATCY 3 cut(s) 568, 1520, 1804
Bsu15I ATCGAT 1 cut(s) 2121
BsuRI GGCC 6 cut(s) 171, 480, 642, 673, 1720, 1866
BsuTUI ATCGAT 1 cut(s) 2121
BtsCI GGATG 5 cut(s) 570, 989, 1357, 2047, 2150
BtsI GCAGTG 1 cut(s) 1018
BtsIMutI CAGTG 8 cut(s) 270, 312, 797, 860, 1018, 1419, 2108, 2292
BveI ACCTGC 1 cut(s) 1733
Cac8I GCNNGC 5 cut(s) 6, 671, 922, 1180, 1227
CciI TCATGA 1 cut(s) 2251
CfoI GCGC 1 cut(s) 1942
Cfr10I RCCGGY 2 cut(s) 238, 744
Cfr13I GGNCC 1 cut(s) 1466
ClaI ATCGAT 1 cut(s) 2121
CseI GACGC 2 cut(s) 86, 2095
CsiI ACCWGGT 1 cut(s) 1813
Csp6I GTAC 7 cut(s) 190, 730, 938, 1134, 1474, 1482, 2201
CspAI ACCGGT 2 cut(s) 238, 744
CviQI GTAC 7 cut(s) 190, 730, 938, 1134, 1474, 1482, 2201
DdeI CTNAG 3 cut(s) 487, 687, 1310
DinI GGCGCC 1 cut(s) 1941
DpnI GATC 9 cut(s) 537, 570, 631, 852, 1522, 1806, 1890, 2055, 2139
DpnII GATC 9 cut(s) 535, 568, 629, 850, 1520, 1804, 1888, 2053, 2137
DraI TTTAAA 1 cut(s) 1048
EaeI YGGCCR 1 cut(s) 478
Eam1104I CTCTTC 4 cut(s) 970, 1196, 1257, 2310
EarI CTCTTC 4 cut(s) 970, 1196, 1257, 2310
Eco130I CCWWGG 1 cut(s) 1524
Eco47I GGWCC 1 cut(s) 1466
Eco57I CTGAAG 4 cut(s) 237, 1568, 2226, 2326
Eco72I CACGTG 1 cut(s) 2318
Eco88I CYCGRG 1 cut(s) 1780
EcoNI CCTNNNNNAGG 1 cut(s) 543
EcoRII CCWGG 6 cut(s) 276, 463, 1070, 1692, 1813, 2281
EcoT14I CCWWGG 1 cut(s) 1524
EcoT22I ATGCAT 1 cut(s) 2083
EgeI GGCGCC 1 cut(s) 1941
EheI GGCGCC 1 cut(s) 1941
ErhI CCWWGG 1 cut(s) 1524
FalI AAGNNNNNCTT 2 cut(s) 537, 569
FaqI GGGAC 1 cut(s) 1464
FauNDI CATATG 1 cut(s) 1927
FbaI TGATCA 1 cut(s) 1888
FblI GTMKAC 1 cut(s) 749
Fnu4HI GCNGC 3 cut(s) 1176, 2264, 2279
FokI GGATG 5 cut(s) 577, 996, 1364, 2034, 2137
Fsp4HI GCNGC 3 cut(s) 1176, 2264, 2279
FspBI CTAG 6 cut(s) 74, 119, 621, 759, 1829, 1868
GlaI GCGC 1 cut(s) 1941
GluI GCNGC 3 cut(s) 1176, 2264, 2279
HaeII RGCGCY 1 cut(s) 1943
HaeIII GGCC 6 cut(s) 171, 480, 642, 673, 1720, 1866
HapII CCGG 4 cut(s) 239, 481, 745, 2141
HgaI GACGC 2 cut(s) 86, 2095
HhaI GCGC 1 cut(s) 1942
Hin1I GRCGYC 1 cut(s) 1940
Hin6I GCGC 1 cut(s) 1940
HinP1I GCGC 1 cut(s) 1940
HincII GTYRAC 2 cut(s) 324, 798
HindII GTYRAC 2 cut(s) 324, 798
HindIII AAGCTT 1 cut(s) 1604
HpaI GTTAAC 1 cut(s) 798
HpaII CCGG 4 cut(s) 239, 481, 745, 2141
HphI GGTGA 3 cut(s) 122, 1763, 2322
Hpy188I TCNGA 7 cut(s) 688, 979, 1313, 1809, 1858, 1981, 2155
HpyAV CCTTC 6 cut(s) 302, 653, 1157, 1215, 1358, 1362
HpyCH4III ACNGT 4 cut(s) 316, 792, 1358, 1592
HpyCH4IV ACGT 4 cut(s) 752, 897, 970, 2317
HpyF10VI GCNNNNNNNGC 6 cut(s) 77, 309, 845, 1717, 2269, 2278
HpyF3I CTNAG 3 cut(s) 487, 687, 1310
HpySE526I ACGT 4 cut(s) 752, 897, 970, 2317
Hsp92I GRCGYC 1 cut(s) 1940
HspAI GCGC 1 cut(s) 1940
KasI GGCGCC 1 cut(s) 1939
Kpn2I TCCGGA 1 cut(s) 2140
Ksp22I TGATCA 1 cut(s) 1888
KspAI GTTAAC 1 cut(s) 798
Kzo9I GATC 9 cut(s) 535, 568, 629, 850, 1520, 1804, 1888, 2053, 2137
LguI GCTCTTC 2 cut(s) 1196, 1257
LmnI GCTCC 4 cut(s) 525, 605, 826, 1668
Lsp1109I GCAGC 3 cut(s) 1162, 2250, 2290
LweI GCATC 2 cut(s) 1177, 1405
MabI ACCWGGT 1 cut(s) 1813
MaeI CTAG 6 cut(s) 74, 119, 621, 759, 1829, 1868
MaeII ACGT 4 cut(s) 752, 897, 970, 2317
MaeIII GTNAC 5 cut(s) 449, 893, 1383, 2318, 2336
MalI GATC 9 cut(s) 537, 570, 631, 852, 1522, 1806, 1890, 2055, 2139
MboI GATC 9 cut(s) 535, 568, 629, 850, 1520, 1804, 1888, 2053, 2137
MfeI CAATTG 1 cut(s) 647
MflI RGATCY 3 cut(s) 568, 1520, 1804
MhlI GDGCHC 1 cut(s) 530
Mly113I GGCGCC 1 cut(s) 1940
MlyI GAGTC 5 cut(s) 267, 991, 1772, 2089, 2296
MmeI TCCRAC 2 cut(s) 1236, 1686
Mph1103I ATGCAT 1 cut(s) 2083
MroI TCCGGA 1 cut(s) 2140
MslI CAYNNNNRTG 2 cut(s) 212, 955
MspI CCGG 4 cut(s) 239, 481, 745, 2141
MspR9I CCNGG 6 cut(s) 278, 465, 1072, 1694, 1815, 2283
MunI CAATTG 1 cut(s) 647
Mva1269I GAATGC 3 cut(s) 1106, 1272, 1620
MvaI CCWGG 6 cut(s) 278, 465, 1072, 1694, 1815, 2283
MwoI GCNNNNNNNGC 6 cut(s) 77, 309, 845, 1717, 2269, 2278
NarI GGCGCC 1 cut(s) 1940
NdeI CATATG 1 cut(s) 1927
NdeII GATC 9 cut(s) 535, 568, 629, 850, 1520, 1804, 1888, 2053, 2137
NlaIV GGNNCC 5 cut(s) 570, 607, 828, 1522, 1941
NmeAIII GCCGAG 1 cut(s) 1949
NmuCI GTSAC 2 cut(s) 449, 2318
NsiI ATGCAT 1 cut(s) 2083
NspI RCATGY 4 cut(s) 924, 1660, 2081, 2224
OliI CACNNNNGTG 1 cut(s) 955
PaeI GCATGC 1 cut(s) 924
PaeR7I CTCGAG 1 cut(s) 1780
PagI TCATGA 1 cut(s) 2251
PciI ACATGT 1 cut(s) 1656
PciSI GCTCTTC 2 cut(s) 1196, 1257
PctI GAATGC 3 cut(s) 1106, 1272, 1620
PfeI GAWTC 9 cut(s) 150, 248, 349, 371, 772, 1281, 1529, 1962, 2312
PflMI CCANNNNNTGG 1 cut(s) 178
PinAI ACCGGT 2 cut(s) 238, 744
PkrI GCNGC 3 cut(s) 1177, 2265, 2280
PleI GAGTC 5 cut(s) 266, 991, 1772, 2089, 2296
PluTI GGCGCC 1 cut(s) 1943
PmaCI CACGTG 1 cut(s) 2318
PmlI CACGTG 1 cut(s) 2318
PpsI GAGTC 5 cut(s) 266, 991, 1772, 2089, 2296
Ppu21I YACGTR 1 cut(s) 2318
PscI ACATGT 1 cut(s) 1656
PshBI ATTAAT 1 cut(s) 1643
PsiI TTATAA 1 cut(s) 1293
Psp6I CCWGG 6 cut(s) 276, 463, 1070, 1692, 1813, 2281
PspCI CACGTG 1 cut(s) 2318
PspGI CCWGG 6 cut(s) 276, 463, 1070, 1692, 1813, 2281
PspN4I GGNNCC 5 cut(s) 570, 607, 828, 1522, 1941
PspPI GGNCC 1 cut(s) 1466
PstI CTGCAG 1 cut(s) 346
PsuI RGATCY 3 cut(s) 568, 1520, 1804
RsaI GTAC 7 cut(s) 191, 731, 939, 1135, 1475, 1483, 2202
RsaNI GTAC 7 cut(s) 190, 730, 938, 1134, 1474, 1482, 2201
RseI CAYNNNNRTG 2 cut(s) 212, 955
SapI GCTCTTC 2 cut(s) 1196, 1257
SatI GCNGC 3 cut(s) 1176, 2264, 2279
Sau3AI GATC 9 cut(s) 535, 568, 629, 850, 1520, 1804, 1888, 2053, 2137
Sau96I GGNCC 1 cut(s) 1466
SchI GAGTC 5 cut(s) 267, 991, 1772, 2089, 2296
ScrFI CCNGG 6 cut(s) 278, 465, 1072, 1694, 1815, 2283
SduI GDGCHC 1 cut(s) 530
SexAI ACCWGGT 1 cut(s) 1813
SfaNI GCATC 2 cut(s) 1177, 1405
SfcI CTRYAG 2 cut(s) 342, 1407
SfoI GGCGCC 1 cut(s) 1941
Sfr274I CTCGAG 1 cut(s) 1780
SinI GGWCC 1 cut(s) 1466
SlaI CTCGAG 1 cut(s) 1780
SmiI ATTTAAAT 1 cut(s) 1048
SmiMI CAYNNNNRTG 2 cut(s) 212, 955
SmlI CTYRAG 3 cut(s) 543, 1094, 1780
SmoI CTYRAG 3 cut(s) 543, 1094, 1780
SphI GCATGC 1 cut(s) 924
SsiI CCGC 2 cut(s) 1388, 1458
SspDI GGCGCC 1 cut(s) 1939
SspI AATATT 3 cut(s) 1641, 1735, 1822
SspMI CTAG 6 cut(s) 74, 119, 621, 759, 1829, 1868
StyD4I CCNGG 6 cut(s) 276, 463, 1070, 1692, 1813, 2281
StyI CCWWGG 1 cut(s) 1524
SwaI ATTTAAAT 1 cut(s) 1048
TaaI ACNGT 4 cut(s) 316, 792, 1358, 1592
TaiI ACGT 4 cut(s) 755, 900, 973, 2320
TaqI TCGA 4 cut(s) 164, 1713, 1781, 2121
TatI WGTACW 2 cut(s) 729, 1473
TfiI GAWTC 9 cut(s) 150, 248, 349, 371, 772, 1281, 1529, 1962, 2312
TscAI CASTG 8 cut(s) 277, 319, 797, 860, 1018, 1426, 2115, 2299
TseFI GTSAC 2 cut(s) 449, 2318
TseI GCWGC 3 cut(s) 1175, 2263, 2278
Tsp45I GTSAC 2 cut(s) 449, 2318
TspGWI ACGGA 5 cut(s) 37, 577, 765, 1312, 2314
TspRI CASTG 8 cut(s) 277, 319, 797, 860, 1018, 1426, 2115, 2299
Van91I CCANNNNNTGG 1 cut(s) 178
VpaK11BI GGWCC 1 cut(s) 1466
VspI ATTAAT 1 cut(s) 1643
XagI CCTNNNNNAGG 1 cut(s) 543
XapI RAATTY 2 cut(s) 861, 1147
XbaI TCTAGA 1 cut(s) 758
XceI RCATGY 4 cut(s) 924, 1660, 2081, 2224
XcmI CCANNNNNNNNNTGG 1 cut(s) 1669
XhoI CTCGAG 1 cut(s) 1780
XmiI GTMKAC 1 cut(s) 749
XspI CTAG 6 cut(s) 74, 119, 621, 759, 1829, 1868
Zsp2I ATGCAT 1 cut(s) 2083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.