Rw5G017230

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000207
Physical Location & Seq
Reverse (-)
21978046 .. 21979772
1727 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G017230.1

Sequence Viewer

Length: 1092 bp
ATGCTAAAGAGATCTACTCTTTCAGAAGCCCGAAGAAAGCTGGATTCCAGAAAGGGGGATTACCGTGGAGAAGACCAGGAAGACTTGGAGTTACCATTATTTGACTTGACCACTATTGCTAATGCCACTGATGACTTTTCAAGCAGCAACAAGCTGGGTGAAGGTGGTTTTGGGCCTGTGTACAAGGGAACTTTGGTTGGAGGAGAGGAAATTGCAGTAAAGAGGCTCTCAAATAACTCTGGACAGGGATTGAGAGAGTTTAAAAACGAAGTTCTACTGATAGCCAAACTTCAGCATAGGAATCTTGTAAAGCTTCTTGGTTGTTGCATTCAAGAAGATGAAGAAATTTTAGTATATGAATTCATGCCTAACAGAAGCTTGGACTTATTTATCTTTGATCAAGAAAGACAGAAGTTTCTCGACTGGCCCACGTGCTTCCACATTATTGACGGAATTGCTCGAGGGCTTCTTTATCTCCATCAAGACTCTAGATTAAGAATTATCCATAGGGATATGAAGGCTAGCAATATCTTGCTAGACAATGATATGAACCCAAAGATTTCGGACTTCGGACTGGCTAAAACATTTGGGGGTGATCAAAGTGAAGACAGTACAAATAGAGTAGTTGGAACACATGGTTATATGGCTCCTGAATATGCAGTAGATGGAACTTTCTCAATGAAATCTGATGTATTTAGCTTTGGAGTTATTATGCTAGAGATATTGAGCAGGAAGAAGAACAGGGGATTTTGTCATCCAGATCACCGTCTCAACCTTCTTGGACATGCATGGACACTATGGAATCAAAATAATCCTCTGGAAATGGTTGATGTGGCGTTATGTGATTCTTGCAACATAACCGAAGTGTTAAGGTGTCTTCATGTGGGCCTGTTATGTGTGCAACAAGTACCTGAAGATAGACCAAGCATGTCATCTGTGGTTCTAATGTTGGGCAGTGAAGTTAAATTGCCTTCACCAAAGCAACCTGGTTTTTACACTGGACGGACTGTGACAGTCCCCGAATCAACATCATCAACGACCGACTTGTTTTCAACAAATATTTGCAGCACAATGTTTGTAGAGGCTCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

363

Amino Acids

40.82

Weight (kDa)

5.55

Isoelectric Point (pI)

53.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 47 - 246 3.9e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 49 - 314 7.2e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27300
fragaria_vesca FvH4_3g15930 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g15980 FvH4_3g21350 FvH4_3g21400 FvH4_3g21400 FvH4_6g12332
malus_domestica MD02G1167100.v1.1 MD02G1167200.v1.1 MD03G1185600.v1.1 MD05G1213900.v1.1 MD05G1214100.v1.1 MD05G1214200.v1.1 MD05G1214700.v1.1 MD05G1216300.v1.1 MD05G1216800.v1.1 MD05G1217300.v1.1 MD05G1218000.v1.1 MD11G1231100.v1.1 MD11G1231200.v1.1 MD11G1231400.v1.1 MD11G1231500.v1.1 MD11G1232400.v1.1 MD11G1232500.v1.1
prunus_persica Prupe.4G142300_v2.0.a1 Prupe.4G142400_v2.0.a1 Prupe.4G142500_v2.0.a1 Prupe.4G142600_v2.0.a1 Prupe.4G142800_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195000_v2.0.a1 Prupe.4G195100_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195300_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195400_v2.0.a1 Prupe.4G195500_v2.0.a1 Prupe.4G195700_v2.0.a1 Prupe.4G195900_v2.0.a1
pyrus_communis pycom03g16540 pycom05g19920 pycom11g20460 pycom11g20480 pycom11g20490 pycom11g20500 pycom11g20530
rosa_chinensis RchiOBHm_Chr2g0139311 RchiOBHm_Chr2g0139351 RchiOBHm_Chr5g0026801 RchiOBHm_Chr5g0026851 RchiOBHm_Chr5g0026891 RchiOBHm_Chr5g0026911 RchiOBHm_Chr5g0036571 RchiOBHm_Chr5g0036591 RchiOBHm_Chr5g0036721 RchiOBHm_Chr5g0036741 RchiOBHm_Chr7g0222981
rosa_laevigata RLG00000002006 RLG00000019807 RLG00000032964 RLG00000032968 RLG00000032969 RLG00000033706 RLG00000033708 RLG00000033712 RLG00000033716
rosa_multiflora Rmu_co8256651.1_g000001 Rmu_co8279377.1_g000001 Rmu_co8429919.1_g000001 Rmu_co8490103.1_g000001 Rmu_sc0000774.1_g000002 Rmu_sc0002525.1_g000012 Rmu_sc0004223.1_g000008 Rmu_sc0004390.1_g000002 Rmu_sc0006059.1_g000014 Rmu_sc0006059.1_g000024 Rmu_sc0006514.1_g000020 Rmu_sc0014333.1_g000002 Rmu_ssc0000172.1_g000018 Rmu_ssc0000172.1_g000029
rosa_roxburghii Rroxscaffold_1G00044230 Rroxscaffold_1G00044260 Rroxscaffold_1G00044320 Rroxscaffold_1G00044340 Rroxscaffold_1G00052560 Rroxscaffold_1G00052570 Rroxscaffold_1G00052660 Rroxscaffold_1G00052720 Rroxscaffold_2G00105450 Rroxscaffold_2G00105460
rosa_rugosa Rorug02G0356900 Rorug02G0357000 Rorug02G0357100 Rorug02G0357200 Rorug02G0357300 Rorug02G0357400 Rorug02G0357500 Rorug02G0357600 Rorug05G0095400 Rorug05G0095500 Rorug05G0095500 Rorug05G0095600 Rorug05G0095700 Rorug05G0095800 Rorug05G0157700 Rorug05G0157800
rosa_samantha Rh2AG406800 Rh2AG406900 Rh2BG417300 Rh2CG392900 Rh2DG426200 Rh5AG189300 Rh5AG249400 Rh5AG250100 Rh5BG186300 Rh5BG186900 Rh5BG187000 Rh5BG249500 Rh5BG250600 Rh5BG250900 Rh5BG251500 Rh5BG251800 Rh5CG205500 Rh5CG206300 Rh5CG206700 Rh5CG283000 Rh5CG283300 Rh5CG283500 Rh5DG188500 Rh5DG188700 Rh5DG258700 Rh5DG258900 Rh5DG259600 Rh5DG259900 Rh7CG375800 Rh7DG352500
rosa_wichuraiana Rw2G032960 Rw2G032970 Rw2G033220 Rw2G033230 Rw5G017130 Rw5G017230 Rw5G022940 Rw5G022960 Rw5G022980 Rw7G030420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 345, 359
AcuI CTGAAG 2 cut(s) 275, 933
AcvI CACGTG 1 cut(s) 432
AfaI GTAC 3 cut(s) 182, 613, 909
AfiI CCNNNNNNNGG 1 cut(s) 54
AgsI TTSAA 3 cut(s) 141, 332, 1053
AjnI CCWGG 2 cut(s) 75, 985
AjuI GAANNNNNNNTTGG 2 cut(s) 153, 185
AluBI AGCT 5 cut(s) 40, 154, 313, 378, 699
AluI AGCT 5 cut(s) 40, 154, 313, 378, 699
Alw26I GTCTC 1 cut(s) 773
Ama87I CYCGRG 1 cut(s) 459
AoxI GGCC 3 cut(s) 173, 425, 886
ApeKI GCWGC 2 cut(s) 144, 1065
ApoI RAATTY 2 cut(s) 345, 359
AspS9I GGNCC 3 cut(s) 173, 426, 886
AsuHPI GGTGA 4 cut(s) 170, 605, 755, 966
AsuNHI GCTAGC 1 cut(s) 521
AvaI CYCGRG 1 cut(s) 459
BbrPI CACGTG 1 cut(s) 432
BbsI GAAGAC 4 cut(s) 78, 87, 612, 869
BbvI GCAGC 2 cut(s) 156, 1077
BccI CCATC 2 cut(s) 486, 659
BciT130I CCWGG 2 cut(s) 77, 987
BclI TGATCA 2 cut(s) 397, 595
BcoDI GTCTC 1 cut(s) 773
BfaI CTAG 5 cut(s) 489, 522, 536, 716, 1090
BglII AGATCT 1 cut(s) 11
BisI GCNGC 2 cut(s) 145, 1066
BlsI GCNGC 2 cut(s) 146, 1067
Bme1390I CCNGG 2 cut(s) 77, 987
BmeT110I CYCGRG 1 cut(s) 459
BmgT120I GGNCC 3 cut(s) 173, 426, 886
BmiI GGNNCC 1 cut(s) 648
BmrFI CCNGG 2 cut(s) 77, 987
BmtI GCTAGC 1 cut(s) 525
BpiI GAAGAC 4 cut(s) 78, 87, 612, 869
BplI GAGNNNNNCTC 1 cut(s) 33
BsaAI YACGTR 1 cut(s) 432
BsaJI CCNNGG 1 cut(s) 64
Bsc4I CCNNNNNNNGG 1 cut(s) 54
Bse1I ACTGG 3 cut(s) 428, 579, 1003
BseBI CCWGG 2 cut(s) 77, 987
BseDI CCNNGG 1 cut(s) 64
BseGI GGATG 1 cut(s) 754
BseLI CCNNNNNNNGG 1 cut(s) 54
BseNI ACTGG 3 cut(s) 428, 579, 1003
BseRI GAGGAG 1 cut(s) 216
BseXI GCAGC 2 cut(s) 156, 1077
BseYI CCCAGC 1 cut(s) 154
Bsh1285I CGRYCG 1 cut(s) 1041
BshFI GGCC 3 cut(s) 175, 427, 888
BsiEI CGRYCG 1 cut(s) 1041
BsiHKCI CYCGRG 1 cut(s) 459
BslFI GGGAC 1 cut(s) 1001
BslI CCNNNNNNNGG 1 cut(s) 54
BsmAI GTCTC 1 cut(s) 773
BsmBI CGTCTC 1 cut(s) 773
BsmFI GGGAC 1 cut(s) 1001
BsmI GAATGC 1 cut(s) 327
BsnI GGCC 3 cut(s) 175, 427, 888
BsoBI CYCGRG 1 cut(s) 459
Bsp1407I TGTACA 1 cut(s) 180
Bsp143I GATC 4 cut(s) 11, 397, 595, 760
BspANI GGCC 3 cut(s) 175, 427, 888
BspLI GGNNCC 1 cut(s) 648
BspOI GCTAGC 1 cut(s) 525
BsrGI TGTACA 1 cut(s) 180
BsrI ACTGG 3 cut(s) 428, 579, 1003
BssECI CCNNGG 1 cut(s) 64
BssMI GATC 4 cut(s) 11, 397, 595, 760
Bst2UI CCWGG 2 cut(s) 77, 987
Bst4CI ACNGT 5 cut(s) 65, 611, 767, 1009, 1015
BstAUI TGTACA 1 cut(s) 180
BstBAI YACGTR 1 cut(s) 432
BstC8I GCNNGC 2 cut(s) 523, 1087
BstDSI CCRYGG 1 cut(s) 64
BstF5I GGATG 1 cut(s) 754
BstKTI GATC 4 cut(s) 14, 400, 598, 763
BstMAI GTCTC 1 cut(s) 773
BstMBI GATC 4 cut(s) 11, 397, 595, 760
BstMCI CGRYCG 1 cut(s) 1041
BstNI CCWGG 2 cut(s) 77, 987
BstNSI RCATGY 2 cut(s) 788, 931
BstSCI CCNGG 2 cut(s) 75, 985
BstV1I GCAGC 2 cut(s) 156, 1077
BstV2I GAAGAC 4 cut(s) 78, 87, 612, 869
BstX2I RGATCY 1 cut(s) 11
BstYI RGATCY 1 cut(s) 11
BsuRI GGCC 3 cut(s) 175, 427, 888
BtgI CCRYGG 1 cut(s) 64
BtsCI GGATG 1 cut(s) 754
BtsI GCAGTG 1 cut(s) 961
BtsIMutI CAGTG 3 cut(s) 126, 961, 996
Cac8I GCNNGC 2 cut(s) 523, 1087
Cfr13I GGNCC 3 cut(s) 173, 426, 886
CsiI ACCWGGT 1 cut(s) 985
Csp6I GTAC 3 cut(s) 181, 612, 908
CviAII CATG 6 cut(s) 364, 635, 785, 789, 881, 928
CviQI GTAC 3 cut(s) 181, 612, 908
DpnI GATC 4 cut(s) 13, 399, 597, 762
DpnII GATC 4 cut(s) 11, 397, 595, 760
DraI TTTAAA 1 cut(s) 262
Eco57I CTGAAG 2 cut(s) 275, 933
Eco72I CACGTG 1 cut(s) 432
Eco88I CYCGRG 1 cut(s) 459
EcoRI GAATTC 1 cut(s) 359
EcoRII CCWGG 2 cut(s) 75, 985
EcoT22I ATGCAT 1 cut(s) 790
Esp3I CGTCTC 1 cut(s) 773
FaeI CATG 6 cut(s) 367, 638, 788, 792, 884, 931
FaqI GGGAC 1 cut(s) 1001
FatI CATG 6 cut(s) 363, 634, 784, 788, 880, 927
FbaI TGATCA 2 cut(s) 397, 595
Fnu4HI GCNGC 2 cut(s) 145, 1066
FokI GGATG 1 cut(s) 741
Fsp4HI GCNGC 2 cut(s) 145, 1066
FspBI CTAG 5 cut(s) 489, 522, 536, 716, 1090
GluI GCNGC 2 cut(s) 145, 1066
GsaI CCCAGC 1 cut(s) 158
HaeIII GGCC 3 cut(s) 175, 427, 888
Hin1II CATG 6 cut(s) 367, 638, 788, 792, 884, 931
HindIII AAGCTT 2 cut(s) 311, 376
HinfI GANTC 6 cut(s) 44, 301, 485, 802, 845, 1022
HphI GGTGA 4 cut(s) 170, 605, 755, 966
Hpy166II GTNNAC 1 cut(s) 181
Hpy188I TCNGA 4 cut(s) 25, 565, 572, 688
Hpy8I GTNNAC 1 cut(s) 181
HpyAV CCTTC 4 cut(s) 155, 511, 785, 981
HpyCH4III ACNGT 5 cut(s) 65, 611, 767, 1009, 1015
HpyCH4IV ACGT 1 cut(s) 431
HpyCH4V TGCA 7 cut(s) 215, 327, 659, 788, 852, 901, 1065
HpySE526I ACGT 1 cut(s) 431
Hsp92II CATG 6 cut(s) 367, 638, 788, 792, 884, 931
Ksp22I TGATCA 2 cut(s) 397, 595
Kzo9I GATC 4 cut(s) 11, 397, 595, 760
LmnI GCTCC 1 cut(s) 652
Lsp1109I GCAGC 2 cut(s) 156, 1077
MabI ACCWGGT 1 cut(s) 985
MaeI CTAG 5 cut(s) 489, 522, 536, 716, 1090
MaeII ACGT 1 cut(s) 431
MaeIII GTNAC 2 cut(s) 90, 1009
MalI GATC 4 cut(s) 13, 399, 597, 762
MboI GATC 4 cut(s) 11, 397, 595, 760
MflI RGATCY 1 cut(s) 11
MluCI AATT 6 cut(s) 210, 345, 359, 453, 498, 965
MlyI GAGTC 1 cut(s) 479
MmeI TCCRAC 2 cut(s) 178, 607
MnlI CCTC 6 cut(s) 194, 199, 216, 455, 825, 1075
Mph1103I ATGCAT 1 cut(s) 790
MseI TTAA 4 cut(s) 261, 494, 869, 963
MspR9I CCNGG 2 cut(s) 77, 987
Mva1269I GAATGC 1 cut(s) 327
MvaI CCWGG 2 cut(s) 77, 987
NdeII GATC 4 cut(s) 11, 397, 595, 760
NheI GCTAGC 1 cut(s) 521
NlaIII CATG 6 cut(s) 367, 638, 788, 792, 884, 931
NlaIV GGNNCC 1 cut(s) 648
NmuCI GTSAC 1 cut(s) 1009
NsiI ATGCAT 1 cut(s) 790
NspI RCATGY 2 cut(s) 788, 931
PaeR7I CTCGAG 1 cut(s) 459
PctI GAATGC 1 cut(s) 327
PfeI GAWTC 5 cut(s) 44, 301, 802, 845, 1022
PkrI GCNGC 2 cut(s) 146, 1067
PleI GAGTC 1 cut(s) 479
PmaCI CACGTG 1 cut(s) 432
PmlI CACGTG 1 cut(s) 432
PpsI GAGTC 1 cut(s) 479
Ppu21I YACGTR 1 cut(s) 432
Psp6I CCWGG 2 cut(s) 75, 985
PspCI CACGTG 1 cut(s) 432
PspFI CCCAGC 1 cut(s) 154
PspGI CCWGG 2 cut(s) 75, 985
PspN4I GGNNCC 1 cut(s) 648
PspPI GGNCC 3 cut(s) 173, 426, 886
PspXI VCTCGAGB 1 cut(s) 459
PsuI RGATCY 1 cut(s) 11
RsaI GTAC 3 cut(s) 182, 613, 909
RsaNI GTAC 3 cut(s) 181, 612, 908
SaqAI TTAA 4 cut(s) 261, 494, 869, 963
SatI GCNGC 2 cut(s) 145, 1066
Sau3AI GATC 4 cut(s) 11, 397, 595, 760
Sau96I GGNCC 3 cut(s) 173, 426, 886
SchI GAGTC 1 cut(s) 479
ScrFI CCNGG 2 cut(s) 77, 987
SexAI ACCWGGT 1 cut(s) 985
Sfr274I CTCGAG 1 cut(s) 459
SlaI CTCGAG 1 cut(s) 459
SmlI CTYRAG 1 cut(s) 459
SmoI CTYRAG 1 cut(s) 459
Sse9I AATT 6 cut(s) 210, 345, 359, 453, 498, 965
SspI AATATT 1 cut(s) 1060
SspMI CTAG 5 cut(s) 489, 522, 536, 716, 1090
StyD4I CCNGG 2 cut(s) 75, 985
TaaI ACNGT 5 cut(s) 65, 611, 767, 1009, 1015
TaiI ACGT 1 cut(s) 434
TaqI TCGA 2 cut(s) 420, 460
TaqII GACCGA 1 cut(s) 1055
TasI AATT 6 cut(s) 210, 345, 359, 453, 498, 965
TatI WGTACW 2 cut(s) 180, 611
TfiI GAWTC 5 cut(s) 44, 301, 802, 845, 1022
Tru1I TTAA 4 cut(s) 261, 494, 869, 963
Tru9I TTAA 4 cut(s) 261, 494, 869, 963
TscAI CASTG 3 cut(s) 133, 961, 1003
TseFI GTSAC 1 cut(s) 1009
TseI GCWGC 2 cut(s) 144, 1065
Tsp45I GTSAC 1 cut(s) 1009
TspDTI ATGAA 7 cut(s) 352, 354, 372, 530, 563, 695, 869
TspGWI ACGGA 2 cut(s) 465, 1018
TspRI CASTG 3 cut(s) 133, 961, 1003
XapI RAATTY 2 cut(s) 345, 359
XbaI TCTAGA 1 cut(s) 488
XceI RCATGY 2 cut(s) 788, 931
XhoI CTCGAG 1 cut(s) 459
XspI CTAG 5 cut(s) 489, 522, 536, 716, 1090
Zsp2I ATGCAT 1 cut(s) 790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.