Serine threonine-protein kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 269 |
| Acc36I | ACCTGC | 1 cut(s) | 702 |
| AccB1I | GGYRCC | 1 cut(s) | 1240 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 509 |
| AciI | CCGC | 1 cut(s) | 1041 |
| AclI | AACGTT | 1 cut(s) | 1024 |
| AclWI | GGATC | 1 cut(s) | 525 |
| AcoI | YGGCCR | 2 cut(s) | 829, 1016 |
| AcsI | RAATTY | 3 cut(s) | 238, 317, 1149 |
| AcuI | CTGAAG | 1 cut(s) | 863 |
| AfaI | GTAC | 4 cut(s) | 5, 61, 120, 454 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 509, 771, 1086 |
| AflIII | ACRYGT | 1 cut(s) | 1378 |
| AjnI | CCWGG | 3 cut(s) | 288, 966, 1079 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 741, 773 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 395, 427, 900, 932 |
| Alw21I | GWGCWC | 1 cut(s) | 681 |
| Alw26I | GTCTC | 3 cut(s) | 120, 228, 258 |
| AlwI | GGATC | 1 cut(s) | 525 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 509, 520 |
| AoxI | GGCC | 5 cut(s) | 105, 145, 829, 1016, 1420 |
| ApeKI | GCWGC | 4 cut(s) | 285, 459, 506, 912 |
| ApoI | RAATTY | 3 cut(s) | 238, 317, 1149 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 845 |
| AspLEI | GCGC | 2 cut(s) | 270, 464 |
| AspS9I | GGNCC | 3 cut(s) | 105, 761, 1420 |
| AsuC2I | CCSGG | 1 cut(s) | 683 |
| AsuHPI | GGTGA | 4 cut(s) | 191, 370, 593, 644 |
| AvaII | GGWCC | 1 cut(s) | 761 |
| BalI | TGGCCA | 2 cut(s) | 831, 1018 |
| BanI | GGYRCC | 1 cut(s) | 1240 |
| BanII | GRGCYC | 1 cut(s) | 681 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1387, 1419 |
| BauI | CACGAG | 1 cut(s) | 63 |
| BbsI | GAAGAC | 1 cut(s) | 1049 |
| Bbv12I | GWGCWC | 1 cut(s) | 681 |
| BbvI | GCAGC | 4 cut(s) | 272, 446, 493, 899 |
| BccI | CCATC | 7 cut(s) | 238, 299, 473, 782, 1077, 1253, 1356 |
| BciT130I | CCWGG | 3 cut(s) | 290, 968, 1081 |
| BcnI | CCSGG | 1 cut(s) | 683 |
| BcoDI | GTCTC | 3 cut(s) | 120, 228, 258 |
| BfaI | CTAG | 3 cut(s) | 257, 708, 1170 |
| BfmI | CTRYAG | 1 cut(s) | 573 |
| BfuAI | ACCTGC | 1 cut(s) | 702 |
| BglII | AGATCT | 1 cut(s) | 1102 |
| BisI | GCNGC | 4 cut(s) | 286, 460, 507, 913 |
| BlsI | GCNGC | 4 cut(s) | 287, 461, 508, 914 |
| BmcAI | AGTACT | 1 cut(s) | 61 |
| Bme1390I | CCNGG | 4 cut(s) | 290, 683, 968, 1081 |
| Bme18I | GGWCC | 1 cut(s) | 761 |
| BmgT120I | GGNCC | 3 cut(s) | 105, 761, 1420 |
| BmiI | GGNNCC | 4 cut(s) | 157, 660, 763, 1242 |
| BmrFI | CCNGG | 4 cut(s) | 290, 683, 968, 1081 |
| BmsI | GCATC | 2 cut(s) | 892, 899 |
| BpiI | GAAGAC | 1 cut(s) | 1049 |
| BpmI | CTGGAG | 2 cut(s) | 695, 1331 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1085 |
| BpuEI | CTTGAG | 3 cut(s) | 16, 210, 362 |
| BpuMI | CCSGG | 1 cut(s) | 683 |
| BsaAI | YACGTR | 1 cut(s) | 78 |
| BsaI | GGTCTC | 1 cut(s) | 258 |
| BsaJI | CCNNGG | 1 cut(s) | 832 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 509, 771, 1086 |
| Bse1I | ACTGG | 4 cut(s) | 57, 188, 254, 460 |
| BseBI | CCWGG | 3 cut(s) | 290, 968, 1081 |
| BseDI | CCNNGG | 1 cut(s) | 832 |
| BseGI | GGATG | 5 cut(s) | 266, 673, 914, 1288, 1348 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 509, 771, 1086 |
| BseMII | CTCAG | 1 cut(s) | 555 |
| BseNI | ACTGG | 4 cut(s) | 57, 188, 254, 460 |
| BseRI | GAGGAG | 5 cut(s) | 459, 462, 941, 1011, 1200 |
| BseXI | GCAGC | 4 cut(s) | 272, 446, 493, 899 |
| BseYI | CCCAGC | 1 cut(s) | 509 |
| BshFI | GGCC | 5 cut(s) | 107, 147, 831, 1018, 1422 |
| BshNI | GGYRCC | 1 cut(s) | 1240 |
| BsiHKAI | GWGCWC | 1 cut(s) | 681 |
| BsiSI | CCGG | 1 cut(s) | 683 |
| BslFI | GGGAC | 1 cut(s) | 790 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 509, 771, 1086 |
| BsmAI | GTCTC | 3 cut(s) | 120, 228, 258 |
| BsmFI | GGGAC | 1 cut(s) | 790 |
| BsmI | GAATGC | 1 cut(s) | 389 |
| BsnI | GGCC | 5 cut(s) | 107, 147, 831, 1018, 1422 |
| Bso31I | GGTCTC | 1 cut(s) | 258 |
| Bsp1286I | GDGCHC | 1 cut(s) | 681 |
| Bsp1407I | TGTACA | 1 cut(s) | 3 |
| Bsp143I | GATC | 6 cut(s) | 91, 478, 517, 670, 1102, 1189 |
| BspACI | CCGC | 1 cut(s) | 1041 |
| BspANI | GGCC | 5 cut(s) | 107, 147, 831, 1018, 1422 |
| BspCNI | CTCAG | 1 cut(s) | 554 |
| BspLI | GGNNCC | 4 cut(s) | 157, 660, 763, 1242 |
| BspMI | ACCTGC | 1 cut(s) | 702 |
| BspPI | GGATC | 1 cut(s) | 525 |
| BspT107I | GGYRCC | 1 cut(s) | 1240 |
| BspTNI | GGTCTC | 1 cut(s) | 258 |
| BsrGI | TGTACA | 1 cut(s) | 3 |
| BsrI | ACTGG | 4 cut(s) | 57, 188, 254, 460 |
| BssECI | CCNNGG | 1 cut(s) | 832 |
| BssMI | GATC | 6 cut(s) | 91, 478, 517, 670, 1102, 1189 |
| BssSI | CACGAG | 1 cut(s) | 63 |
| BssT1I | CCWWGG | 1 cut(s) | 832 |
| Bst2BI | CACGAG | 1 cut(s) | 63 |
| Bst2UI | CCWGG | 3 cut(s) | 290, 968, 1081 |
| Bst4CI | ACNGT | 5 cut(s) | 279, 703, 1008, 1232, 1361 |
| Bst6I | CTCTTC | 1 cut(s) | 803 |
| BstAUI | TGTACA | 1 cut(s) | 3 |
| BstBAI | YACGTR | 1 cut(s) | 78 |
| BstC8I | GCNNGC | 1 cut(s) | 537 |
| BstDEI | CTNAG | 2 cut(s) | 541, 1085 |
| BstF5I | GGATG | 5 cut(s) | 266, 673, 914, 1288, 1348 |
| BstHHI | GCGC | 2 cut(s) | 270, 464 |
| BstKTI | GATC | 6 cut(s) | 94, 481, 520, 673, 1105, 1192 |
| BstMAI | GTCTC | 3 cut(s) | 120, 228, 258 |
| BstMBI | GATC | 6 cut(s) | 91, 478, 517, 670, 1102, 1189 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 1419 |
| BstNI | CCWGG | 3 cut(s) | 290, 968, 1081 |
| BstNSI | RCATGY | 2 cut(s) | 955, 1382 |
| BstSCI | CCNGG | 4 cut(s) | 288, 681, 966, 1079 |
| BstSFI | CTRYAG | 1 cut(s) | 573 |
| BstV1I | GCAGC | 4 cut(s) | 272, 446, 493, 899 |
| BstV2I | GAAGAC | 1 cut(s) | 1049 |
| BstX2I | RGATCY | 2 cut(s) | 517, 1102 |
| BstYI | RGATCY | 2 cut(s) | 517, 1102 |
| BsuRI | GGCC | 5 cut(s) | 107, 147, 831, 1018, 1422 |
| BtsCI | GGATG | 5 cut(s) | 266, 673, 914, 1288, 1348 |
| BveI | ACCTGC | 1 cut(s) | 702 |
| Cac8I | GCNNGC | 1 cut(s) | 537 |
| CaiI | CAGNNNCTG | 2 cut(s) | 509, 520 |
| CfoI | GCGC | 2 cut(s) | 270, 464 |
| Cfr13I | GGNCC | 3 cut(s) | 105, 761, 1420 |
| Csp6I | GTAC | 4 cut(s) | 4, 60, 119, 453 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1019, 1054 |
| CviAII | CATG | 2 cut(s) | 952, 1379 |
| CviQI | GTAC | 4 cut(s) | 4, 60, 119, 453 |
| DdeI | CTNAG | 2 cut(s) | 541, 1085 |
| DpnI | GATC | 6 cut(s) | 93, 480, 519, 672, 1104, 1191 |
| DpnII | GATC | 6 cut(s) | 91, 478, 517, 670, 1102, 1189 |
| EaeI | YGGCCR | 2 cut(s) | 829, 1016 |
| Eam1104I | CTCTTC | 1 cut(s) | 803 |
| EarI | CTCTTC | 1 cut(s) | 803 |
| Ecl136II | GAGCTC | 1 cut(s) | 679 |
| Eco130I | CCWWGG | 1 cut(s) | 832 |
| Eco24I | GRGCYC | 1 cut(s) | 681 |
| Eco31I | GGTCTC | 1 cut(s) | 258 |
| Eco32I | GATATC | 2 cut(s) | 439, 490 |
| Eco47I | GGWCC | 1 cut(s) | 761 |
| Eco53kI | GAGCTC | 1 cut(s) | 679 |
| Eco57I | CTGAAG | 1 cut(s) | 863 |
| EcoICRI | GAGCTC | 1 cut(s) | 679 |
| EcoO109I | RGGNCCY | 1 cut(s) | 105 |
| EcoRII | CCWGG | 3 cut(s) | 288, 966, 1079 |
| EcoRV | GATATC | 2 cut(s) | 439, 490 |
| EcoT14I | CCWWGG | 1 cut(s) | 832 |
| EcoT22I | ATGCAT | 1 cut(s) | 1415 |
| EcoT38I | GRGCYC | 1 cut(s) | 681 |
| ErhI | CCWWGG | 1 cut(s) | 832 |
| FaeI | CATG | 2 cut(s) | 955, 1382 |
| FaqI | GGGAC | 1 cut(s) | 790 |
| FatI | CATG | 2 cut(s) | 951, 1378 |
| Fnu4HI | GCNGC | 4 cut(s) | 286, 460, 507, 913 |
| FokI | GGATG | 5 cut(s) | 273, 680, 921, 1295, 1335 |
| FriOI | GRGCYC | 1 cut(s) | 681 |
| Fsp4HI | GCNGC | 4 cut(s) | 286, 460, 507, 913 |
| FspBI | CTAG | 3 cut(s) | 257, 708, 1170 |
| FspI | TGCGCA | 1 cut(s) | 269 |
| GlaI | GCGC | 2 cut(s) | 269, 463 |
| GluI | GCNGC | 4 cut(s) | 286, 460, 507, 913 |
| GsaI | CCCAGC | 1 cut(s) | 513 |
| GsuI | CTGGAG | 2 cut(s) | 695, 1331 |
| HaeIII | GGCC | 5 cut(s) | 107, 147, 831, 1018, 1422 |
| HapII | CCGG | 1 cut(s) | 683 |
| HhaI | GCGC | 2 cut(s) | 270, 464 |
| Hin1II | CATG | 2 cut(s) | 955, 1382 |
| Hin6I | GCGC | 2 cut(s) | 268, 462 |
| HinP1I | GCGC | 2 cut(s) | 268, 462 |
| HindIII | AAGCTT | 2 cut(s) | 875, 899 |
| HinfI | GANTC | 8 cut(s) | 184, 210, 373, 971, 1076, 1141, 1341, 1386 |
| HpaII | CCGG | 1 cut(s) | 683 |
| HphI | GGTGA | 4 cut(s) | 191, 370, 593, 644 |
| Hpy166II | GTNNAC | 1 cut(s) | 769 |
| Hpy188I | TCNGA | 6 cut(s) | 436, 443, 544, 746, 1156, 1282 |
| Hpy188III | TCNNGA | 8 cut(s) | 33, 65, 227, 674, 819, 850, 1073, 1100 |
| Hpy8I | GTNNAC | 1 cut(s) | 769 |
| HpyAV | CCTTC | 3 cut(s) | 20, 608, 743 |
| HpyCH4III | ACNGT | 5 cut(s) | 279, 703, 1008, 1232, 1361 |
| HpyCH4IV | ACGT | 2 cut(s) | 77, 1024 |
| HpyCH4V | TGCA | 3 cut(s) | 387, 872, 1413 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 1419 |
| HpyF3I | CTNAG | 2 cut(s) | 541, 1085 |
| HpySE526I | ACGT | 2 cut(s) | 77, 1024 |
| Hsp92II | CATG | 2 cut(s) | 955, 1382 |
| HspAI | GCGC | 2 cut(s) | 268, 462 |
| Kzo9I | GATC | 6 cut(s) | 91, 478, 517, 670, 1102, 1189 |
| LmnI | GCTCC | 3 cut(s) | 155, 676, 684 |
| Lsp1109I | GCAGC | 4 cut(s) | 272, 446, 493, 899 |
| LweI | GCATC | 2 cut(s) | 892, 899 |
| MaeI | CTAG | 3 cut(s) | 257, 708, 1170 |
| MaeII | ACGT | 2 cut(s) | 77, 1024 |
| MalI | GATC | 6 cut(s) | 93, 480, 519, 672, 1104, 1191 |
| MboI | GATC | 6 cut(s) | 91, 478, 517, 670, 1102, 1189 |
| MboII | GAAGA | 4 cut(s) | 820, 1049, 1236, 1395 |
| MfeI | CAATTG | 1 cut(s) | 406 |
| MflI | RGATCY | 2 cut(s) | 517, 1102 |
| MhlI | GDGCHC | 1 cut(s) | 681 |
| MlsI | TGGCCA | 2 cut(s) | 831, 1018 |
| MluCI | AATT | 9 cut(s) | 139, 238, 317, 406, 617, 798, 1149, 1179, 1398 |
| MluNI | TGGCCA | 2 cut(s) | 831, 1018 |
| MlyI | GAGTC | 1 cut(s) | 965 |
| MmeI | TCCRAC | 2 cut(s) | 568, 1201 |
| Mox20I | TGGCCA | 2 cut(s) | 831, 1018 |
| Mph1103I | ATGCAT | 1 cut(s) | 1415 |
| MroXI | GAANNNNTTC | 1 cut(s) | 845 |
| MscI | TGGCCA | 2 cut(s) | 831, 1018 |
| MseI | TTAA | 3 cut(s) | 281, 620, 1178 |
| Msp20I | TGGCCA | 2 cut(s) | 831, 1018 |
| MspA1I | CMGCKG | 1 cut(s) | 506 |
| MspI | CCGG | 1 cut(s) | 683 |
| MspR9I | CCNGG | 4 cut(s) | 290, 683, 968, 1081 |
| MunI | CAATTG | 1 cut(s) | 406 |
| Mva1269I | GAATGC | 1 cut(s) | 389 |
| MvaI | CCWGG | 3 cut(s) | 290, 968, 1081 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 1419 |
| NciI | CCSGG | 1 cut(s) | 683 |
| NdeII | GATC | 6 cut(s) | 91, 478, 517, 670, 1102, 1189 |
| NlaIII | CATG | 2 cut(s) | 955, 1382 |
| NlaIV | GGNNCC | 4 cut(s) | 157, 660, 763, 1242 |
| NsbI | TGCGCA | 1 cut(s) | 269 |
| NsiI | ATGCAT | 1 cut(s) | 1415 |
| NspI | RCATGY | 2 cut(s) | 955, 1382 |
| PciI | ACATGT | 1 cut(s) | 1378 |
| PctI | GAATGC | 1 cut(s) | 389 |
| PdmI | GAANNNNTTC | 1 cut(s) | 845 |
| PfeI | GAWTC | 7 cut(s) | 184, 210, 373, 1076, 1141, 1341, 1386 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 509 |
| PkrI | GCNGC | 4 cut(s) | 287, 461, 508, 914 |
| PleI | GAGTC | 1 cut(s) | 965 |
| PpsI | GAGTC | 1 cut(s) | 965 |
| Ppu21I | YACGTR | 1 cut(s) | 78 |
| PscI | ACATGT | 1 cut(s) | 1378 |
| Psp124BI | GAGCTC | 1 cut(s) | 681 |
| Psp1406I | AACGTT | 1 cut(s) | 1024 |
| Psp6I | CCWGG | 3 cut(s) | 288, 966, 1079 |
| PspFI | CCCAGC | 1 cut(s) | 509 |
| PspGI | CCWGG | 3 cut(s) | 288, 966, 1079 |
| PspN4I | GGNNCC | 4 cut(s) | 157, 660, 763, 1242 |
| PspPI | GGNCC | 3 cut(s) | 105, 761, 1420 |
| PstNI | CAGNNNCTG | 2 cut(s) | 509, 520 |
| PsuI | RGATCY | 2 cut(s) | 517, 1102 |
| PvuII | CAGCTG | 1 cut(s) | 506 |
| RsaI | GTAC | 4 cut(s) | 5, 61, 120, 454 |
| RsaNI | GTAC | 4 cut(s) | 4, 60, 119, 453 |
| SacI | GAGCTC | 1 cut(s) | 681 |
| SaqAI | TTAA | 3 cut(s) | 281, 620, 1178 |
| SatI | GCNGC | 4 cut(s) | 286, 460, 507, 913 |
| Sau3AI | GATC | 6 cut(s) | 91, 478, 517, 670, 1102, 1189 |
| Sau96I | GGNCC | 3 cut(s) | 105, 761, 1420 |
| ScaI | AGTACT | 1 cut(s) | 61 |
| SchI | GAGTC | 1 cut(s) | 965 |
| ScrFI | CCNGG | 4 cut(s) | 290, 683, 968, 1081 |
| SduI | GDGCHC | 1 cut(s) | 681 |
| SfaNI | GCATC | 2 cut(s) | 892, 899 |
| SfcI | CTRYAG | 1 cut(s) | 573 |
| SinI | GGWCC | 1 cut(s) | 761 |
| SmlI | CTYRAG | 3 cut(s) | 31, 225, 377 |
| SmoI | CTYRAG | 3 cut(s) | 31, 225, 377 |
| Sse9I | AATT | 9 cut(s) | 139, 238, 317, 406, 617, 798, 1149, 1179, 1398 |
| SsiI | CCGC | 1 cut(s) | 1041 |
| SspMI | CTAG | 3 cut(s) | 257, 708, 1170 |
| SstI | GAGCTC | 1 cut(s) | 681 |
| StyD4I | CCNGG | 4 cut(s) | 288, 681, 966, 1079 |
| StyI | CCWWGG | 1 cut(s) | 832 |
| TaaI | ACNGT | 5 cut(s) | 279, 703, 1008, 1232, 1361 |
| TaiI | ACGT | 2 cut(s) | 80, 1027 |
| TaqI | TCGA | 2 cut(s) | 886, 1099 |
| TasI | AATT | 9 cut(s) | 139, 238, 317, 406, 617, 798, 1149, 1179, 1398 |
| TatI | WGTACW | 2 cut(s) | 3, 59 |
| TfiI | GAWTC | 7 cut(s) | 184, 210, 373, 1076, 1141, 1341, 1386 |
| Tru1I | TTAA | 3 cut(s) | 281, 620, 1178 |
| Tru9I | TTAA | 3 cut(s) | 281, 620, 1178 |
| TseI | GCWGC | 4 cut(s) | 285, 459, 506, 912 |
| TspDTI | ATGAA | 8 cut(s) | 102, 386, 659, 870, 960, 1085, 1149, 1154 |
| TspGWI | ACGGA | 2 cut(s) | 13, 544 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 509 |
| VpaK11BI | GGWCC | 1 cut(s) | 761 |
| XapI | RAATTY | 3 cut(s) | 238, 317, 1149 |
| XceI | RCATGY | 2 cut(s) | 955, 1382 |
| XmnI | GAANNNNTTC | 1 cut(s) | 845 |
| XspI | CTAG | 3 cut(s) | 257, 708, 1170 |
| ZrmI | AGTACT | 1 cut(s) | 61 |
| Zsp2I | ATGCAT | 1 cut(s) | 1415 |