FvH4_3g25080

Ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000157
Physical Location & Seq
Reverse (-)
18119732 .. 18122551
2820 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g25080.t1

Sequence Viewer

Length: 1848 bp
ATGTTTACACAGTTTGTTCAATCTGGAACCATGTCGTCTAACCCACAAGCACAACAGGTGCCACCTCTTGCAGGGATTCAACTAGCAACCACCACCATCGACATTCCGGCAGATTTACCTGTACCCAGGACACCTTCGCTTCATCTACTAGAACACAAAAACAGAGAGCTATACCTTGAGATATGCGTTCCCCTCTACAAGTATGCACTAAAAGGTGATTGGGAAGCTGCGGAACAGATCTTGGAAAAGGATCGGACACTTTTGAGTGCTGGCATATCAAGAGGATGGGACACTGTTCTTCATATTGCAGCAGGAGCAAGACATGTTCACTTTGTGGAGAAACTTGCCGAAATCATGGACAAAGAACACTTGGAACTCCAAGACAAAAATGGAAACACTGCGTTATGTATCGCTGCCGCGGCAGGAGCTGTAGAGATTGTTGAGATTTTGATAAAACATAACGATTCTTTGCTGACACGTCGAGGAAGTCATAAAATGGCACCACCCTATATGGCTGCTGTTTTAGGACAATCCGAAATGGCATGGTACCTGTACCCTAAAAGTAAAAAGATGCTGGAGAAAACCGACCTTGAGAACTTGTTCTTTTCGTGCATCAACAATGGTCTGTATGATTTAGCCTTACAGTTGCTAGATACTGATACATCATTAGCCAAGGCACGGACTGATCGGCCGAAAAATAAAACAGCCTTGCATATCTTGGCTCGAAGGCCTTCAGTATTTGGATCCCAAAGTCCAGGAATCTGGGGCAGACTCATTAAATCATTCCTCCCAGGTTTTAAGTTTGCATTCACAAGGAATTTAAATCAAGCTGATCAAGCACTTCAGCTGGTCGAACGCCTTTGGGAAGAAATTTTGAAAGATGAGCATGATGACGTGATGAGGCTGATCAAATATCCTTCAAATTTAATATTTGACGCAGCAGAACTAGGGAATTATGAGTTCCTGGCTGTGCTTATGAGCTCATATCCTGAGCTAGTATGGGAAACTGATAACAAAAATCGAACTATCATCCATGTCGCAGTTTTGCATCGCCATGCTAGTATCTTCAATCTTGTTCATGAGATAGGATCTATCAAGGACGTCATAGTGACGTATGAGGATAAAGAGGGTAACAATATAGTGCATATGGCTGCAAAAGTAGCACCTCAAAACCAACTGAACCTGGTATCGGGGGTAGCTCTTCAAATGCAACGAGAGTTGGTATGGTTTGAGGAAGTAAAAAAGATTGTTCAACCTCAATATATAGAGATGGAAAACAAGAAAGGAAAAACACCACAAGAATTATTCACGAAAGAGCATAGGACGTTAATGCGTCAAGGAGAGAAATGGATGAAGGAAACTGCAACTTCATGCTTGCTTGTTGCGACTATTATTGCAACTGTTGTGTTTTCAGCTGCGTTTAGCATACCGGGAGGCACAGATGATAAAACTGGAAAGCCAAACTTTTTGACAGAGAAAGCTTTTCTATACTTCACCATAGCCGACGGAGTGGCACTGTTTTCTTCTTCAACTGCAATGCTGATGTTCTTGTTCATCCTTACCTCGCGGTATGCGGAAAACGATTTCCTCATGTCCTTGCCCTTGAAGTTGATGGTTGGACTATCTTGCCTCTTCATCTCAATAGCATCTATGATGATGGCTTTCAGCACTGCCTTCTATTTGTCTTGTCAATATGGATCAAAATGGGTTCCGGATCTTACATTTTTCTTTGCAATTGTCCCTGTTGCTTTATATGCATTTCTGCAGTTCCCTCTTGTGTCTGATATGTCATCTTCAACGTTTTGTTCAAGTCTTCTATTTCAGCCATGGAAACATATGATATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

616

Amino Acids

69.38

Weight (kDa)

6.4

Isoelectric Point (pI)

43.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 69 - 154 1.4e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 100 - 187 7.5e-10 Ankyrin repeats (3 copies)
PGG PF13962 448 - 561 2.7e-25 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54070 AT5G35830
fragaria_vesca FvH4_3g25070 FvH4_3g25080 FvH4_3g25081 FvH4_3g25081
malus_domestica MD03G1027500.v1.1 MD03G1146300.v1.1 MD03G1146800.v1.1 MD03G1146900.v1.1 MD03G1147100.v1.1 MD11G1165600.v1.1
prunus_persica Prupe.2G015800_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G016300_v2.0.a1 Prupe.2G016500_v2.0.a1 Prupe.6G131100_v2.0.a1 Prupe.6G131400_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1
pyrus_communis pycom03g10090 pycom03g10110 pycom11g13740 pycom11g13770
rosa_chinensis RchiOBHm_Chr1g0316371 RchiOBHm_Chr1g0316381 RchiOBHm_Chr1g0316401 RchiOBHm_Chr1g0316411 RchiOBHm_Chr1g0316571 RchiOBHm_Chr1g0316601 RchiOBHm_Chr5g0039341 RchiOBHm_Chr5g0041371 RchiOBHm_Chr5g0041401 RchiOBHm_Chr5g0041431 RchiOBHm_Chr5g0041471 RchiOBHm_Chr5g0045281 RchiOBHm_Chr5g0045291 RchiOBHm_Chr5g0045311 RchiOBHm_Chr5g0045371 RchiOBHm_Chr5g0045421 RchiOBHm_Chr5g0045431 RchiOBHm_Chr5g0045501 RchiOBHm_Chr5g0045551 RchiOBHm_Chr5g0045771 RchiOBHm_Chr5g0045811
rosa_laevigata RLG00000034057 RLG00000034058
rosa_multiflora Rmu_co8352751.1_g000001 Rmu_co8397575.1_g000001 Rmu_sc0000415.1_g000006 Rmu_sc0000493.1_g000057 Rmu_sc0000504.1_g000003 Rmu_sc0000594.1_g000019 Rmu_sc0000594.1_g000032 Rmu_sc0000594.1_g000066 Rmu_sc0001047.1_g000003 Rmu_sc0001047.1_g000011 Rmu_sc0001556.1_g000023 Rmu_sc0002584.1_g000003 Rmu_sc0002584.1_g000039 Rmu_sc0002584.1_g000070 Rmu_sc0002667.1_g000015 Rmu_sc0003677.1_g000003 Rmu_sc0003743.1_g000017 Rmu_sc0004520.1_g000004 Rmu_sc0004520.1_g000013 Rmu_sc0004520.1_g000027 Rmu_sc0005539.1_g000014 Rmu_sc0005539.1_g000032 Rmu_sc0006018.1_g000006 Rmu_sc0006737.1_g000020 Rmu_sc0007485.1_g000002 Rmu_sc0008729.1_g000002 Rmu_sc0008729.1_g000003 Rmu_sc0038822.1_g000001 Rmu_ssc0000398.1_g000025
rosa_roxburghii Rroxscaffold_1G00035090 Rroxscaffold_1G00035630 Rroxscaffold_1G00035640 Rroxscaffold_1G00035660 Rroxscaffold_1G00035680 Rroxscaffold_1G00035730 Rroxscaffold_1G00035750 Rroxscaffold_1G00035770 Rroxscaffold_1G00039150 Rroxscaffold_1G00039190 Rroxscaffold_1G00039220 Rroxscaffold_1G00039260 Rroxscaffold_4G00328910 Rroxscaffold_4G00328950 Rroxscaffold_4G00328960 Rroxscaffold_4G00330890
rosa_rugosa Rorug01G0009800 Rorug01G0010000.1 Rorug01G0010200 Rorug01G0012100 Rorug01G0023500 Rorug01G0023600 Rorug01G0023700 Rorug01G0024700 Rorug05G0192000 Rorug05G0192200 Rorug05G0222400 Rorug05G0222500 Rorug05G0222600.1 Rorug05G0222700.1 Rorug05G0222800.1 Rorug05G0222900.1 Rorug05G0223000.1 Rorug05G0223100.1 Rorug05G0223200 Rorug05G0223600 Rorug05G0226800 Rorug05G0226900
rosa_samantha Rh1CG019600 Rh1DG015000 Rh1DG015300 Rh1DG016900 Rh5CG315200 Rh5CG338700 Rh5DG277100 Rh5DG291500 Rh5DG292100 Rh5DG292200 Rh5DG292400 Rh5DG292600 Rh5DG322100 Rh5DG322200 Rh5DG323100 Rh5DG323300 Rh5DG326700 Rh5DG326900 Rh5DG327000
rosa_wichuraiana Rw0G021580 Rw1G001320 Rw1G001420 Rw1G002820 Rw1G002840 Rw1G002870 Rw5G024800 Rw5G026160 Rw5G026170 Rw5G026180 Rw5G026210 Rw5G026230 Rw5G028170 Rw5G028190 Rw5G028210 Rw5G028230 Rw5G028240 Rw5G028260 Rw5G028280 Rw5G028370 Rw5G028380 Rw5G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1104
Acc65I GGTACC 1 cut(s) 546
AccB1I GGYRCC 3 cut(s) 58, 499, 546
AccII CGCG 2 cut(s) 419, 1567
AccIII TCCGGA 1 cut(s) 1711
AciI CCGC 5 cut(s) 230, 417, 419, 1567, 1574
AclI AACGTT 1 cut(s) 1799
AclWI GGATC 6 cut(s) 258, 738, 751, 1096, 1705, 1722
AcoI YGGCCR 1 cut(s) 689
AcsI RAATTY 3 cut(s) 817, 870, 922
AcuI CTGAAG 2 cut(s) 717, 827
AcyI GRCGYC 1 cut(s) 1101
AdeI CACNNNGTG 1 cut(s) 334
AfaI GTAC 3 cut(s) 123, 548, 554
AfiI CCNNNNNNNGG 3 cut(s) 71, 678, 1189
AflIII ACRYGT 2 cut(s) 322, 476
AjiI CACGTC 2 cut(s) 479, 895
AjnI CCWGG 5 cut(s) 125, 754, 790, 963, 1182
Alw21I GWGCWC 1 cut(s) 983
AlwI GGATC 6 cut(s) 258, 738, 751, 1096, 1705, 1722
AlwNI CAGNNNCTG 1 cut(s) 428
Aor13HI TCCGGA 1 cut(s) 1711
AoxI GGCC 2 cut(s) 689, 728
ApeKI GCWGC 7 cut(s) 227, 308, 413, 515, 938, 1151, 1415
ApoI RAATTY 3 cut(s) 817, 870, 922
Asp700I GAANNNNTTC 2 cut(s) 599, 730
Asp718I GGTACC 1 cut(s) 546
AsuC2I CCSGG 1 cut(s) 1431
AsuHPI GGTGA 2 cut(s) 227, 1486
BamHI GGATCC 1 cut(s) 743
BanI GGYRCC 3 cut(s) 58, 499, 546
BanII GRGCYC 1 cut(s) 983
BbsI GAAGAC 1 cut(s) 1805
Bbv12I GWGCWC 1 cut(s) 983
BbvI GCAGC 7 cut(s) 214, 320, 400, 502, 950, 1138, 1402
BccI CCATC 5 cut(s) 104, 279, 1264, 1606, 1651
BciT130I CCWGG 5 cut(s) 127, 756, 792, 965, 1184
BclI TGATCA 2 cut(s) 832, 906
BcnI CCSGG 1 cut(s) 1431
BfaI CTAG 7 cut(s) 83, 149, 650, 947, 995, 1059, 1846
BfmI CTRYAG 2 cut(s) 429, 1763
BglII AGATCT 1 cut(s) 237
BisI GCNGC 9 cut(s) 228, 309, 414, 417, 420, 516, 939, 1152, 1416
BlsI GCNGC 9 cut(s) 229, 310, 415, 418, 421, 517, 940, 1153, 1417
Bme1390I CCNGG 6 cut(s) 127, 756, 792, 965, 1184, 1431
BmgBI CACGTC 2 cut(s) 479, 895
BmiI GGNNCC 6 cut(s) 28, 60, 501, 548, 745, 1710
BmrFI CCNGG 6 cut(s) 127, 756, 792, 965, 1184, 1431
BmsI GCATC 4 cut(s) 561, 621, 1057, 1655
BpiI GAAGAC 1 cut(s) 1805
BpmI CTGGAG 1 cut(s) 596
Bpu10I CCTNAGC 1 cut(s) 990
BpuEI CTTGAG 2 cut(s) 197, 611
BpuMI CCSGG 1 cut(s) 1431
BsaHI GRCGYC 1 cut(s) 1101
BsaJI CCNNGG 5 cut(s) 125, 417, 672, 790, 1826
BsaWI WCCGGW 1 cut(s) 1711
Bsc4I CCNNNNNNNGG 3 cut(s) 71, 678, 1189
Bse1I ACTGG 1 cut(s) 1456
Bse3DI GCAATG 1 cut(s) 1542
BseAI TCCGGA 1 cut(s) 1711
BseBI CCWGG 5 cut(s) 127, 756, 792, 965, 1184
BseDI CCNNGG 5 cut(s) 125, 417, 672, 790, 1826
BseGI GGATG 4 cut(s) 290, 1029, 1356, 1554
BseLI CCNNNNNNNGG 3 cut(s) 71, 678, 1189
BseMI GCAATG 1 cut(s) 1542
BseMII CTCAG 1 cut(s) 981
BseNI ACTGG 1 cut(s) 1456
BseX3I CGGCCG 1 cut(s) 689
BseXI GCAGC 7 cut(s) 214, 320, 400, 502, 950, 1138, 1402
Bsh1236I CGCG 2 cut(s) 419, 1567
Bsh1285I CGRYCG 1 cut(s) 692
BshFI GGCC 2 cut(s) 691, 730
BshNI GGYRCC 3 cut(s) 58, 499, 546
BsiEI CGRYCG 1 cut(s) 692
BsiHKAI GWGCWC 1 cut(s) 983
BsiSI CCGG 3 cut(s) 107, 1430, 1712
BslFI GGGAC 2 cut(s) 302, 1724
BslI CCNNNNNNNGG 3 cut(s) 71, 678, 1189
BsmFI GGGAC 2 cut(s) 302, 1724
BsmI GAATGC 1 cut(s) 806
BsnI GGCC 2 cut(s) 691, 730
Bsp1286I GDGCHC 1 cut(s) 983
Bsp13I TCCGGA 1 cut(s) 1711
Bsp143I GATC 9 cut(s) 237, 250, 685, 743, 832, 906, 1088, 1697, 1714
Bsp19I CCATGG 1 cut(s) 1826
BspACI CCGC 5 cut(s) 230, 417, 419, 1567, 1574
BspANI GGCC 2 cut(s) 691, 730
BspCNI CTCAG 1 cut(s) 982
BspEI TCCGGA 1 cut(s) 1711
BspFNI CGCG 2 cut(s) 419, 1567
BspHI TCATGA 1 cut(s) 1078
BspLI GGNNCC 6 cut(s) 28, 60, 501, 548, 745, 1710
BspMAI CTGCAG 1 cut(s) 1767
BspPI GGATC 6 cut(s) 258, 738, 751, 1096, 1705, 1722
BspQI GCTCTTC 1 cut(s) 1206
BspT107I GGYRCC 3 cut(s) 58, 499, 546
BsrDI GCAATG 1 cut(s) 1542
BsrI ACTGG 1 cut(s) 1456
BssECI CCNNGG 5 cut(s) 125, 417, 672, 790, 1826
BssMI GATC 9 cut(s) 237, 250, 685, 743, 832, 906, 1088, 1697, 1714
BssNI GRCGYC 1 cut(s) 1101
BssT1I CCWWGG 2 cut(s) 672, 1826
Bst2UI CCWGG 5 cut(s) 127, 756, 792, 965, 1184
Bst4CI ACNGT 5 cut(s) 12, 295, 645, 1402, 1518
Bst6I CTCTTC 2 cut(s) 1206, 1637
BstACI GRCGYC 1 cut(s) 1101
BstC8I GCNNGC 2 cut(s) 271, 1376
BstDEI CTNAG 1 cut(s) 990
BstDSI CCRYGG 2 cut(s) 417, 1826
BstENI CCTNNNNNAGG 1 cut(s) 69
BstF5I GGATG 4 cut(s) 290, 1029, 1356, 1554
BstFNI CGCG 2 cut(s) 419, 1567
BstKTI GATC 9 cut(s) 240, 253, 688, 746, 835, 909, 1091, 1700, 1717
BstMBI GATC 9 cut(s) 237, 250, 685, 743, 832, 906, 1088, 1697, 1714
BstMCI CGRYCG 1 cut(s) 692
BstMWI GCNNNNNNNGC 6 cut(s) 314, 419, 425, 836, 1160, 1754
BstNI CCWGG 5 cut(s) 127, 756, 792, 965, 1184
BstNSI RCATGY 1 cut(s) 326
BstSCI CCNGG 6 cut(s) 125, 754, 790, 963, 1182, 1429
BstSFI CTRYAG 2 cut(s) 429, 1763
BstUI CGCG 2 cut(s) 419, 1567
BstV1I GCAGC 7 cut(s) 214, 320, 400, 502, 950, 1138, 1402
BstV2I GAAGAC 1 cut(s) 1805
BstX2I RGATCY 4 cut(s) 237, 743, 1088, 1714
BstXI CCANNNNNNTGG 1 cut(s) 762
BstYI RGATCY 4 cut(s) 237, 743, 1088, 1714
BstZI CGGCCG 1 cut(s) 689
BsuRI GGCC 2 cut(s) 691, 730
BtgI CCRYGG 2 cut(s) 417, 1826
BtgZI GCGATG 1 cut(s) 1034
BtrI CACGTC 2 cut(s) 479, 895
BtsCI GGATG 4 cut(s) 290, 1029, 1356, 1554
BtsI GCAGTG 2 cut(s) 396, 1668
BtsIMutI CAGTG 4 cut(s) 291, 396, 1514, 1668
Cac8I GCNNGC 2 cut(s) 271, 1376
CaiI CAGNNNCTG 1 cut(s) 428
CciI TCATGA 1 cut(s) 1078
Cfr42I CCGCGG 1 cut(s) 420
CseI GACGC 2 cut(s) 944, 1322
CsiI ACCWGGT 1 cut(s) 1182
Csp6I GTAC 3 cut(s) 122, 547, 553
CviQI GTAC 3 cut(s) 122, 547, 553
DdeI CTNAG 1 cut(s) 990
DpnI GATC 9 cut(s) 239, 252, 687, 745, 834, 908, 1090, 1699, 1716
DpnII GATC 9 cut(s) 237, 250, 685, 743, 832, 906, 1088, 1697, 1714
DraI TTTAAA 1 cut(s) 822
DraIII CACNNNGTG 1 cut(s) 334
EaeI YGGCCR 1 cut(s) 689
EagI CGGCCG 1 cut(s) 689
Eam1104I CTCTTC 2 cut(s) 1206, 1637
EarI CTCTTC 2 cut(s) 1206, 1637
Ecl136II GAGCTC 1 cut(s) 981
EclXI CGGCCG 1 cut(s) 689
Eco130I CCWWGG 2 cut(s) 672, 1826
Eco147I AGGCCT 1 cut(s) 730
Eco24I GRGCYC 1 cut(s) 983
Eco52I CGGCCG 1 cut(s) 689
Eco53kI GAGCTC 1 cut(s) 981
Eco57I CTGAAG 2 cut(s) 717, 827
EcoICRI GAGCTC 1 cut(s) 981
EcoNI CCTNNNNNAGG 1 cut(s) 69
EcoRII CCWGG 5 cut(s) 125, 754, 790, 963, 1182
EcoT14I CCWWGG 2 cut(s) 672, 1826
EcoT22I ATGCAT 1 cut(s) 1759
EcoT38I GRGCYC 1 cut(s) 983
ErhI CCWWGG 2 cut(s) 672, 1826
FalI AAGNNNNNCTT 2 cut(s) 1448, 1480
FaqI GGGAC 2 cut(s) 302, 1724
FauNDI CATATG 2 cut(s) 1146, 1836
FbaI TGATCA 2 cut(s) 832, 906
Fnu4HI GCNGC 9 cut(s) 228, 309, 414, 417, 420, 516, 939, 1152, 1416
FokI GGATG 4 cut(s) 297, 1016, 1363, 1541
FriOI GRGCYC 1 cut(s) 983
Fsp4HI GCNGC 9 cut(s) 228, 309, 414, 417, 420, 516, 939, 1152, 1416
FspBI CTAG 7 cut(s) 83, 149, 650, 947, 995, 1059, 1846
GluI GCNGC 9 cut(s) 228, 309, 414, 417, 420, 516, 939, 1152, 1416
GsuI CTGGAG 1 cut(s) 596
HaeIII GGCC 2 cut(s) 691, 730
HapII CCGG 3 cut(s) 107, 1430, 1712
HgaI GACGC 2 cut(s) 944, 1322
Hin1I GRCGYC 1 cut(s) 1101
HindIII AAGCTT 1 cut(s) 1479
HinfI GANTC 4 cut(s) 76, 464, 759, 771
HpaII CCGG 3 cut(s) 107, 1430, 1712
HphI GGTGA 2 cut(s) 227, 1486
Hpy166II GTNNAC 2 cut(s) 6, 328
Hpy188I TCNGA 3 cut(s) 255, 535, 1783
Hpy188III TCNNGA 6 cut(s) 24, 279, 989, 1079, 1309, 1712
Hpy8I GTNNAC 2 cut(s) 6, 328
Hpy99I CGWCG 2 cut(s) 483, 1508
HpyAV CCTTC 6 cut(s) 144, 720, 741, 927, 1348, 1684
HpyCH4III ACNGT 5 cut(s) 12, 295, 645, 1402, 1518
HpyCH4IV ACGT 6 cut(s) 478, 894, 1101, 1112, 1325, 1799
HpyF10VI GCNNNNNNNGC 6 cut(s) 314, 419, 425, 836, 1160, 1754
HpyF3I CTNAG 1 cut(s) 990
HpySE526I ACGT 6 cut(s) 478, 894, 1101, 1112, 1325, 1799
Hsp92I GRCGYC 1 cut(s) 1101
Kpn2I TCCGGA 1 cut(s) 1711
KpnI GGTACC 1 cut(s) 550
Ksp22I TGATCA 2 cut(s) 832, 906
KspI CCGCGG 1 cut(s) 420
Kzo9I GATC 9 cut(s) 237, 250, 685, 743, 832, 906, 1088, 1697, 1714
LguI GCTCTTC 1 cut(s) 1206
LmnI GCTCC 2 cut(s) 314, 425
Lsp1109I GCAGC 7 cut(s) 214, 320, 400, 502, 950, 1138, 1402
LweI GCATC 4 cut(s) 561, 621, 1057, 1655
MabI ACCWGGT 1 cut(s) 1182
MaeI CTAG 7 cut(s) 83, 149, 650, 947, 995, 1059, 1846
MaeII ACGT 6 cut(s) 478, 894, 1101, 1112, 1325, 1799
MaeIII GTNAC 2 cut(s) 1108, 1130
MalI GATC 9 cut(s) 239, 252, 687, 745, 834, 908, 1090, 1699, 1716
MboI GATC 9 cut(s) 237, 250, 685, 743, 832, 906, 1088, 1697, 1714
MboII GAAGA 9 cut(s) 290, 878, 1057, 1193, 1515, 1518, 1624, 1785, 1805
MfeI CAATTG 1 cut(s) 1734
MflI RGATCY 4 cut(s) 237, 743, 1088, 1714
MhlI GDGCHC 1 cut(s) 983
MluCI AATT 6 cut(s) 817, 870, 922, 952, 1301, 1734
MlyI GAGTC 1 cut(s) 765
MmeI TCCRAC 1 cut(s) 1597
Mph1103I ATGCAT 1 cut(s) 1759
MroI TCCGGA 1 cut(s) 1711
MroXI GAANNNNTTC 2 cut(s) 599, 730
MseI TTAA 5 cut(s) 777, 798, 821, 926, 1328
MslI CAYNNNNRTG 1 cut(s) 1053
MspA1I CMGCKG 3 cut(s) 419, 847, 1415
MspI CCGG 3 cut(s) 107, 1430, 1712
MspR9I CCNGG 6 cut(s) 127, 756, 792, 965, 1184, 1431
MunI CAATTG 1 cut(s) 1734
Mva1269I GAATGC 1 cut(s) 806
MvaI CCWGG 5 cut(s) 127, 756, 792, 965, 1184
MvnI CGCG 2 cut(s) 419, 1567
MwoI GCNNNNNNNGC 6 cut(s) 314, 419, 425, 836, 1160, 1754
NciI CCSGG 1 cut(s) 1431
NcoI CCATGG 1 cut(s) 1826
NdeI CATATG 2 cut(s) 1146, 1836
NdeII GATC 9 cut(s) 237, 250, 685, 743, 832, 906, 1088, 1697, 1714
NlaIV GGNNCC 6 cut(s) 28, 60, 501, 548, 745, 1710
NmuCI GTSAC 1 cut(s) 1108
NsiI ATGCAT 1 cut(s) 1759
NspI RCATGY 1 cut(s) 326
PagI TCATGA 1 cut(s) 1078
PceI AGGCCT 1 cut(s) 730
PciI ACATGT 1 cut(s) 322
PciSI GCTCTTC 1 cut(s) 1206
PctI GAATGC 1 cut(s) 806
PdmI GAANNNNTTC 2 cut(s) 599, 730
PfeI GAWTC 3 cut(s) 76, 464, 759
PfoI TCCNGGA 1 cut(s) 754
PkrI GCNGC 9 cut(s) 229, 310, 415, 418, 421, 517, 940, 1153, 1417
PleI GAGTC 1 cut(s) 765
PpsI GAGTC 1 cut(s) 765
PscI ACATGT 1 cut(s) 322
Psp124BI GAGCTC 1 cut(s) 983
Psp1406I AACGTT 1 cut(s) 1799
Psp6I CCWGG 5 cut(s) 125, 754, 790, 963, 1182
PspGI CCWGG 5 cut(s) 125, 754, 790, 963, 1182
PspN4I GGNNCC 6 cut(s) 28, 60, 501, 548, 745, 1710
PstI CTGCAG 1 cut(s) 1767
PstNI CAGNNNCTG 1 cut(s) 428
PsuI RGATCY 4 cut(s) 237, 743, 1088, 1714
PvuII CAGCTG 2 cut(s) 847, 1415
RsaI GTAC 3 cut(s) 123, 548, 554
RsaNI GTAC 3 cut(s) 122, 547, 553
RseI CAYNNNNRTG 1 cut(s) 1053
SacI GAGCTC 1 cut(s) 983
SacII CCGCGG 1 cut(s) 420
SapI GCTCTTC 1 cut(s) 1206
SaqAI TTAA 5 cut(s) 777, 798, 821, 926, 1328
SatI GCNGC 9 cut(s) 228, 309, 414, 417, 420, 516, 939, 1152, 1416
Sau3AI GATC 9 cut(s) 237, 250, 685, 743, 832, 906, 1088, 1697, 1714
SchI GAGTC 1 cut(s) 765
ScrFI CCNGG 6 cut(s) 127, 756, 792, 965, 1184, 1431
SduI GDGCHC 1 cut(s) 983
SexAI ACCWGGT 1 cut(s) 1182
SfaNI GCATC 4 cut(s) 561, 621, 1057, 1655
SfcI CTRYAG 2 cut(s) 429, 1763
Sfr303I CCGCGG 1 cut(s) 420
SgrBI CCGCGG 1 cut(s) 420
SmiI ATTTAAAT 1 cut(s) 822
SmiMI CAYNNNNRTG 1 cut(s) 1053
SmlI CTYRAG 2 cut(s) 176, 590
SmoI CTYRAG 2 cut(s) 176, 590
Sse9I AATT 6 cut(s) 817, 870, 922, 952, 1301, 1734
SseBI AGGCCT 1 cut(s) 730
SsiI CCGC 5 cut(s) 230, 417, 419, 1567, 1574
SspI AATATT 1 cut(s) 930
SspMI CTAG 7 cut(s) 83, 149, 650, 947, 995, 1059, 1846
SstI GAGCTC 1 cut(s) 983
StuI AGGCCT 1 cut(s) 730
StyD4I CCNGG 6 cut(s) 125, 754, 790, 963, 1182, 1429
StyI CCWWGG 2 cut(s) 672, 1826
SwaI ATTTAAAT 1 cut(s) 822
TaaI ACNGT 5 cut(s) 12, 295, 645, 1402, 1518
TaiI ACGT 6 cut(s) 481, 897, 1104, 1115, 1328, 1802
TaqI TCGA 5 cut(s) 99, 481, 724, 852, 1021
TasI AATT 6 cut(s) 817, 870, 922, 952, 1301, 1734
TauI GCSGC 2 cut(s) 419, 422
TfiI GAWTC 3 cut(s) 76, 464, 759
Tru1I TTAA 5 cut(s) 777, 798, 821, 926, 1328
Tru9I TTAA 5 cut(s) 777, 798, 821, 926, 1328
TscAI CASTG 4 cut(s) 298, 403, 1521, 1675
TseFI GTSAC 1 cut(s) 1108
TseI GCWGC 7 cut(s) 227, 308, 413, 515, 938, 1151, 1415
Tsp45I GTSAC 1 cut(s) 1108
TspDTI ATGAA 7 cut(s) 131, 290, 1067, 1359, 1367, 1543, 1624
TspGWI ACGGA 2 cut(s) 694, 1521
TspRI CASTG 4 cut(s) 298, 403, 1521, 1675
XagI CCTNNNNNAGG 1 cut(s) 69
XapI RAATTY 3 cut(s) 817, 870, 922
XceI RCATGY 1 cut(s) 326
XcmI CCANNNNNNNNNTGG 1 cut(s) 386
XmnI GAANNNNTTC 2 cut(s) 599, 730
XspI CTAG 7 cut(s) 83, 149, 650, 947, 995, 1059, 1846
ZraI GACGTC 1 cut(s) 1102
Zsp2I ATGCAT 1 cut(s) 1759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.