RLG00000034057

Ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000157
Physical Location & Seq
Reverse (-)
35964080 .. 35967396
3317 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034057

Sequence Viewer

Length: 1878 bp
ATGTCTATCCCACAAGCACAACATGCACCACCTTCCCAGATTCAATTAACCACAGCTAGCCGCGATTCAGGGACTAGGATAGACAACCAATCCGCAGACATCGCCATTGACATTTCAGCTGATCATTCGGCTCAACCTAACAGACCGTCGTCTTATTTACTAGAACGTACAAACAGAGAAGAATACCTTAAGACAGGTGTTGCCCTCTATAAATATGCACTAAAAGGCGACTGGGAGCCCGTTGAACTAATCTTGACAAAAAAAAAGGGACTTCTGCGAGCAAGCATATCTAGAGGATATGAAACAGTCCTTCACGTTGCAGCAGGAACAAGCCATATTCACTTTGTGGAGAAACTGGTCAACATAATGGAAGTAGAAGACTTGGCACTGCAGGACATAAATGGAAACACTGCCTTGTGCATTGCTGCTTCGGCTGGATCTCTAGAAATTGTCAAGATTTTGATAGAAAAGAACGAGTCTTTGCTAACAATTAGAGGCGGTAAAGGTGTGGCACCAGCCTATATGGCTGCTGTGTTAGGACAGTCTGAAGTGGCATGCTACCTGCACTGCAAAAGTAGTAAAGAGATGTTGAAGAAAACCGACCGAGAAAACTTGTTTTTTTCTTGCATCAACAATTGTCTATACGATTTGGCCTTTCAGTTGCTAGATGCTGATAAAGGATTAGCGAAGGCACGTACTAATTCGCAGAAAAATAAAACGGCCTTGCATATCCTGGCTCGAAGACCTTCAGCATTTGGTGGCAGCCAAAGTCCACGGATGTGGACTAGACTCATTAACTCATTACTCCCAGGTTTGAAGTTCGCATGCAAAAGAAAGTCGAGAGAAGCTAATGGAGCCCTTCAGCTAGTCAACTGCCTCTGGGACGAAATCTTGAGAGATGAGCATGATGACGTTATGAGCCTGATCAAATATCCTTCAAATTTATTATTTGACGCTGTCGAATCAGGGAATTATGAGTTTTTGGCAACGCTTATGAGCTCTTATCCTGAGTTAGTATGGGAAACTGATGAAAAAAATCGAACCATAATCCATGTCGCAGTTCTGCATCGTCACGCAAGTATCTTCAATCTAGTGCATGAAATAGGCTCAATTAAAGATGTCATTGTGACATATAAAGATGATGAGGGTAACAACATTGTGCATATGGCTGCAAAATTAGCACATCAAAATCAATTGAATCTCGTGTCAGGCGTAGCTCTTCAAATGCATCGAGAGTTGGTATGGTTTGAGGAAGTCAAAAAGACTGTCCAACCCCAGTACATCGAGATGGAAAACGACAACGGAAAAACACCTCAAGAATTATTTACTGAAGAGCATACGGTATTAATGCGTGAAGGAGAAAAATGGATGAAGGACACTGCAACTTCATGCATGCTCGTTGCGACTATTATCGCAACCGTTGTATTCTCAGCTACATTTAGCTTACCGGGTGGCACAGATGATCATACTGGAAAACCAAACCTTTTGAAACAGAAAGCCTTTCTAGACTTTACCATAGCTGACGAAATAGCACTCTTTTCCTCTTCAACTGCAATGCTGATGTTCTTGTTCATCCTTACCTCACGGTATGCAGAAAATGATTTCTGCAAGTCCTTACCCTTGAAGTTGATGGTAGGACTCACTTGCCTTTTCATATCTATAGCATCGATGATGATGGCTTTCAGCACCGCCTTCTATCTATCTTGTCAATACGAATCAAAATGGGTTTCAAATTTTACATTTTTATTTGCATTTGTCCCAGTTGCTTTGTTTGCGTTTATGCAATTCCCTCTCGTGTCCGACATGTCCTCTTCTATATTTTTCTCAAGTCTACTATTTCAGCCATGGAAATACATGCTCTACCAGAAGTCGGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

626

Amino Acids

70.05

Weight (kDa)

6.3

Isoelectric Point (pI)

44.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 76 - 158 8.4e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 104 - 191 4.8e-10 Ankyrin repeats (3 copies)
PGG PF13962 454 - 567 2.6e-27 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54070 AT5G35830
fragaria_vesca FvH4_3g25070 FvH4_3g25080 FvH4_3g25081 FvH4_3g25081
malus_domestica MD03G1027500.v1.1 MD03G1146300.v1.1 MD03G1146800.v1.1 MD03G1146900.v1.1 MD03G1147100.v1.1 MD11G1165600.v1.1
prunus_persica Prupe.2G015800_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G016300_v2.0.a1 Prupe.2G016500_v2.0.a1 Prupe.6G131100_v2.0.a1 Prupe.6G131400_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1
pyrus_communis pycom03g10090 pycom03g10110 pycom11g13740 pycom11g13770
rosa_chinensis RchiOBHm_Chr1g0316371 RchiOBHm_Chr1g0316381 RchiOBHm_Chr1g0316401 RchiOBHm_Chr1g0316411 RchiOBHm_Chr1g0316571 RchiOBHm_Chr1g0316601 RchiOBHm_Chr5g0039341 RchiOBHm_Chr5g0041371 RchiOBHm_Chr5g0041401 RchiOBHm_Chr5g0041431 RchiOBHm_Chr5g0041471 RchiOBHm_Chr5g0045281 RchiOBHm_Chr5g0045291 RchiOBHm_Chr5g0045311 RchiOBHm_Chr5g0045371 RchiOBHm_Chr5g0045421 RchiOBHm_Chr5g0045431 RchiOBHm_Chr5g0045501 RchiOBHm_Chr5g0045551 RchiOBHm_Chr5g0045771 RchiOBHm_Chr5g0045811
rosa_laevigata RLG00000034057 RLG00000034058
rosa_multiflora Rmu_co8352751.1_g000001 Rmu_co8397575.1_g000001 Rmu_sc0000415.1_g000006 Rmu_sc0000493.1_g000057 Rmu_sc0000504.1_g000003 Rmu_sc0000594.1_g000019 Rmu_sc0000594.1_g000032 Rmu_sc0000594.1_g000066 Rmu_sc0001047.1_g000003 Rmu_sc0001047.1_g000011 Rmu_sc0001556.1_g000023 Rmu_sc0002584.1_g000003 Rmu_sc0002584.1_g000039 Rmu_sc0002584.1_g000070 Rmu_sc0002667.1_g000015 Rmu_sc0003677.1_g000003 Rmu_sc0003743.1_g000017 Rmu_sc0004520.1_g000004 Rmu_sc0004520.1_g000013 Rmu_sc0004520.1_g000027 Rmu_sc0005539.1_g000014 Rmu_sc0005539.1_g000032 Rmu_sc0006018.1_g000006 Rmu_sc0006737.1_g000020 Rmu_sc0007485.1_g000002 Rmu_sc0008729.1_g000002 Rmu_sc0008729.1_g000003 Rmu_sc0038822.1_g000001 Rmu_ssc0000398.1_g000025
rosa_roxburghii Rroxscaffold_1G00035090 Rroxscaffold_1G00035630 Rroxscaffold_1G00035640 Rroxscaffold_1G00035660 Rroxscaffold_1G00035680 Rroxscaffold_1G00035730 Rroxscaffold_1G00035750 Rroxscaffold_1G00035770 Rroxscaffold_1G00039150 Rroxscaffold_1G00039190 Rroxscaffold_1G00039220 Rroxscaffold_1G00039260 Rroxscaffold_4G00328910 Rroxscaffold_4G00328950 Rroxscaffold_4G00328960 Rroxscaffold_4G00330890
rosa_rugosa Rorug01G0009800 Rorug01G0010000.1 Rorug01G0010200 Rorug01G0012100 Rorug01G0023500 Rorug01G0023600 Rorug01G0023700 Rorug01G0024700 Rorug05G0192000 Rorug05G0192200 Rorug05G0222400 Rorug05G0222500 Rorug05G0222600.1 Rorug05G0222700.1 Rorug05G0222800.1 Rorug05G0222900.1 Rorug05G0223000.1 Rorug05G0223100.1 Rorug05G0223200 Rorug05G0223600 Rorug05G0226800 Rorug05G0226900
rosa_samantha Rh1CG019600 Rh1DG015000 Rh1DG015300 Rh1DG016900 Rh5CG315200 Rh5CG338700 Rh5DG277100 Rh5DG291500 Rh5DG292100 Rh5DG292200 Rh5DG292400 Rh5DG292600 Rh5DG322100 Rh5DG322200 Rh5DG323100 Rh5DG323300 Rh5DG326700 Rh5DG326900 Rh5DG327000
rosa_wichuraiana Rw0G021580 Rw1G001320 Rw1G001420 Rw1G002820 Rw1G002840 Rw1G002870 Rw5G024800 Rw5G026160 Rw5G026170 Rw5G026180 Rw5G026210 Rw5G026230 Rw5G028170 Rw5G028190 Rw5G028210 Rw5G028230 Rw5G028240 Rw5G028260 Rw5G028280 Rw5G028370 Rw5G028380 Rw5G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 570
AccB1I GGYRCC 1 cut(s) 511
AccI GTMKAC 1 cut(s) 1831
AccII CGCG 1 cut(s) 63
AciI CCGC 4 cut(s) 61, 93, 498, 1689
AclWI GGATC 1 cut(s) 445
AcsI RAATTY 2 cut(s) 940, 1732
AcuI CTGAAG 4 cut(s) 567, 732, 845, 1350
AdeI CACNNNGTG 1 cut(s) 346
AfaI GTAC 3 cut(s) 169, 697, 1280
AfiI CCNNNNNNNGG 1 cut(s) 1870
AflII CTTAAG 1 cut(s) 188
AflIII ACRYGT 1 cut(s) 1803
AjnI CCWGG 2 cut(s) 732, 808
AleI CACNNNNGTG 1 cut(s) 778
AluBI AGCT 9 cut(s) 56, 119, 848, 865, 999, 1217, 1433, 1443, 1520
AluI AGCT 9 cut(s) 56, 119, 848, 865, 999, 1217, 1433, 1443, 1520
Alw21I GWGCWC 1 cut(s) 1001
AlwI GGATC 1 cut(s) 445
AoxI GGCC 2 cut(s) 651, 720
ApeKI GCWGC 5 cut(s) 320, 425, 527, 762, 1169
ApoI RAATTY 2 cut(s) 940, 1732
ArsI GACNNNNNNTTYG 2 cut(s) 1251, 1283
AseI ATTAAT 1 cut(s) 1346
Asp700I GAANNNNTTC 1 cut(s) 745
AsuC2I CCSGG 1 cut(s) 1449
AsuNHI GCTAGC 1 cut(s) 56
BaeI ACNNNNGTAYC 2 cut(s) 1063, 1096
BanI GGYRCC 1 cut(s) 511
BanII GRGCYC 3 cut(s) 240, 859, 1001
BauI CACGAG 2 cut(s) 1202, 1793
BbsI GAAGAC 2 cut(s) 384, 748
Bbv12I GWGCWC 1 cut(s) 1001
BbvI GCAGC 5 cut(s) 332, 412, 514, 774, 1156
BccI CCATC 3 cut(s) 1282, 1624, 1669
BceAI ACGGC 1 cut(s) 735
BciT130I CCWGG 2 cut(s) 734, 810
BclI TGATCA 3 cut(s) 121, 924, 1462
BcnI CCSGG 1 cut(s) 1449
BfmI CTRYAG 2 cut(s) 389, 1659
BfrI CTTAAG 1 cut(s) 188
BfuAI ACCTGC 1 cut(s) 570
BglI GCCNNNNNGGC 1 cut(s) 524
BisI GCNGC 6 cut(s) 61, 321, 426, 528, 763, 1170
BlsI GCNGC 6 cut(s) 62, 322, 427, 529, 764, 1171
Bme1390I CCNGG 3 cut(s) 734, 810, 1449
BmiI GGNNCC 3 cut(s) 237, 513, 856
BmrFI CCNGG 3 cut(s) 734, 810, 1449
BmrI ACTGGG 3 cut(s) 241, 1270, 1754
BmsI GCATC 5 cut(s) 636, 658, 1075, 1237, 1673
BmtI GCTAGC 1 cut(s) 60
BmuI ACTGGG 3 cut(s) 241, 1270, 1754
BoxI GACNNNNGTC 1 cut(s) 148
BpiI GAAGAC 2 cut(s) 384, 748
BpuEI CTTGAG 3 cut(s) 913, 1299, 1810
BpuMI CCSGG 1 cut(s) 1449
Bsa29I ATCGAT 1 cut(s) 1667
BsaAI YACGTR 1 cut(s) 695
BsaJI CCNNGG 3 cut(s) 773, 808, 1844
Bsc4I CCNNNNNNNGG 1 cut(s) 1870
Bse1I ACTGG 5 cut(s) 236, 360, 1276, 1474, 1760
Bse3DI GCAATG 2 cut(s) 420, 1560
BseBI CCWGG 2 cut(s) 734, 810
BseCI ATCGAT 1 cut(s) 1667
BseDI CCNNGG 3 cut(s) 773, 808, 1844
BseGI GGATG 3 cut(s) 783, 1374, 1572
BseLI CCNNNNNNNGG 1 cut(s) 1870
BseMI GCAATG 2 cut(s) 420, 1560
BseMII CTCAG 2 cut(s) 999, 1443
BseNI ACTGG 5 cut(s) 236, 360, 1276, 1474, 1760
BseXI GCAGC 5 cut(s) 332, 412, 514, 774, 1156
BsgI GTGCAG 1 cut(s) 548
Bsh1236I CGCG 1 cut(s) 63
Bsh1285I CGRYCG 1 cut(s) 604
BshFI GGCC 2 cut(s) 653, 722
BshNI GGYRCC 1 cut(s) 511
BshVI ATCGAT 1 cut(s) 1667
BsiEI CGRYCG 1 cut(s) 604
BsiHKAI GWGCWC 1 cut(s) 1001
BsiSI CCGG 1 cut(s) 1448
BslFI GGGAC 4 cut(s) 85, 282, 896, 1742
BslI CCNNNNNNNGG 1 cut(s) 1870
BsmFI GGGAC 4 cut(s) 85, 282, 896, 1742
BsnI GGCC 2 cut(s) 653, 722
Bsp1286I GDGCHC 3 cut(s) 240, 859, 1001
Bsp143I GATC 4 cut(s) 121, 437, 924, 1462
Bsp19I CCATGG 1 cut(s) 1844
BspACI CCGC 4 cut(s) 61, 93, 498, 1689
BspANI GGCC 2 cut(s) 653, 722
BspCNI CTCAG 2 cut(s) 1000, 1442
BspDI ATCGAT 1 cut(s) 1667
BspFNI CGCG 1 cut(s) 63
BspLI GGNNCC 3 cut(s) 237, 513, 856
BspMAI CTGCAG 1 cut(s) 393
BspMI ACCTGC 1 cut(s) 570
BspOI GCTAGC 1 cut(s) 60
BspPI GGATC 1 cut(s) 445
BspQI GCTCTTC 2 cut(s) 1224, 1326
BspT107I GGYRCC 1 cut(s) 511
BspTI CTTAAG 1 cut(s) 188
BsrDI GCAATG 2 cut(s) 420, 1560
BsrI ACTGG 5 cut(s) 236, 360, 1276, 1474, 1760
BssECI CCNNGG 3 cut(s) 773, 808, 1844
BssMI GATC 4 cut(s) 121, 437, 924, 1462
BssSI CACGAG 2 cut(s) 1202, 1793
BssT1I CCWWGG 1 cut(s) 1844
Bst2BI CACGAG 2 cut(s) 1202, 1793
Bst2UI CCWGG 2 cut(s) 734, 810
Bst4CI ACNGT 7 cut(s) 147, 307, 543, 1267, 1342, 1420, 1587
Bst6I CTCTTC 4 cut(s) 1224, 1326, 1549, 1816
BstAFI CTTAAG 1 cut(s) 188
BstAPI GCANNNNNTGC 1 cut(s) 23
BstBAI YACGTR 1 cut(s) 695
BstC8I GCNNGC 6 cut(s) 58, 279, 283, 556, 826, 1394
BstDEI CTNAG 2 cut(s) 1008, 1429
BstDSI CCRYGG 2 cut(s) 773, 1844
BstF5I GGATG 3 cut(s) 783, 1374, 1572
BstFNI CGCG 1 cut(s) 63
BstKTI GATC 4 cut(s) 124, 440, 927, 1465
BstMBI GATC 4 cut(s) 121, 437, 924, 1462
BstMCI CGRYCG 1 cut(s) 604
BstMWI GCNNNNNNNGC 7 cut(s) 23, 101, 431, 524, 854, 1178, 1772
BstNI CCWGG 2 cut(s) 734, 810
BstNSI RCATGY 6 cut(s) 26, 558, 828, 1396, 1807, 1858
BstPAI GACNNNNGTC 1 cut(s) 148
BstSCI CCNGG 3 cut(s) 732, 808, 1447
BstSFI CTRYAG 2 cut(s) 389, 1659
BstUI CGCG 1 cut(s) 63
BstV1I GCAGC 5 cut(s) 332, 412, 514, 774, 1156
BstV2I GAAGAC 2 cut(s) 384, 748
BstX2I RGATCY 1 cut(s) 437
BstXI CCANNNNNNTGG 1 cut(s) 780
BstYI RGATCY 1 cut(s) 437
Bsu15I ATCGAT 1 cut(s) 1667
BsuRI GGCC 2 cut(s) 653, 722
BsuTUI ATCGAT 1 cut(s) 1667
BtgI CCRYGG 2 cut(s) 773, 1844
BtgZI GCGATG 1 cut(s) 85
BtsCI GGATG 3 cut(s) 783, 1374, 1572
BtsI GCAGTG 4 cut(s) 386, 408, 565, 1377
BtsIMutI CAGTG 4 cut(s) 386, 408, 565, 1377
BveI ACCTGC 1 cut(s) 570
Cac8I GCNNGC 6 cut(s) 58, 279, 283, 556, 826, 1394
ClaI ATCGAT 1 cut(s) 1667
CseI GACGC 1 cut(s) 962
Csp6I GTAC 3 cut(s) 168, 696, 1279
CviQI GTAC 3 cut(s) 168, 696, 1279
DdeI CTNAG 2 cut(s) 1008, 1429
DpnI GATC 4 cut(s) 123, 439, 926, 1464
DpnII GATC 4 cut(s) 121, 437, 924, 1462
DraIII CACNNNGTG 1 cut(s) 346
Eam1104I CTCTTC 4 cut(s) 1224, 1326, 1549, 1816
EarI CTCTTC 4 cut(s) 1224, 1326, 1549, 1816
Ecl136II GAGCTC 1 cut(s) 999
Eco130I CCWWGG 1 cut(s) 1844
Eco24I GRGCYC 3 cut(s) 240, 859, 1001
Eco53kI GAGCTC 1 cut(s) 999
Eco57I CTGAAG 4 cut(s) 567, 732, 845, 1350
EcoICRI GAGCTC 1 cut(s) 999
EcoRII CCWGG 2 cut(s) 732, 808
EcoT14I CCWWGG 1 cut(s) 1844
EcoT22I ATGCAT 2 cut(s) 1230, 1394
EcoT38I GRGCYC 3 cut(s) 240, 859, 1001
ErhI CCWWGG 1 cut(s) 1844
FalI AAGNNNNNCTT 2 cut(s) 171, 203
FaqI GGGAC 4 cut(s) 85, 282, 896, 1742
FauNDI CATATG 1 cut(s) 1164
FbaI TGATCA 3 cut(s) 121, 924, 1462
FblI GTMKAC 1 cut(s) 1831
Fnu4HI GCNGC 6 cut(s) 61, 321, 426, 528, 763, 1170
FokI GGATG 3 cut(s) 790, 1381, 1559
FriOI GRGCYC 3 cut(s) 240, 859, 1001
Fsp4HI GCNGC 6 cut(s) 61, 321, 426, 528, 763, 1170
GluI GCNGC 6 cut(s) 61, 321, 426, 528, 763, 1170
HaeIII GGCC 2 cut(s) 653, 722
HapII CCGG 1 cut(s) 1448
HgaI GACGC 1 cut(s) 962
HincII GTYRAC 2 cut(s) 361, 871
HindII GTYRAC 2 cut(s) 361, 871
HinfI GANTC 8 cut(s) 40, 65, 476, 789, 962, 1198, 1638, 1715
HpaII CCGG 1 cut(s) 1448
Hpy166II GTNNAC 5 cut(s) 361, 773, 783, 871, 1832
Hpy188I TCNGA 2 cut(s) 547, 1801
Hpy8I GTNNAC 5 cut(s) 361, 773, 783, 871, 1832
Hpy99I CGWCG 1 cut(s) 151
HpyAV CCTTC 9 cut(s) 42, 320, 682, 756, 869, 945, 1349, 1366, 1702
HpyCH4III ACNGT 7 cut(s) 147, 307, 543, 1267, 1342, 1420, 1587
HpyCH4IV ACGT 4 cut(s) 166, 315, 694, 912
HpyF10VI GCNNNNNNNGC 7 cut(s) 23, 101, 431, 524, 854, 1178, 1772
HpyF3I CTNAG 2 cut(s) 1008, 1429
HpySE526I ACGT 4 cut(s) 166, 315, 694, 912
Ksp22I TGATCA 3 cut(s) 121, 924, 1462
Kzo9I GATC 4 cut(s) 121, 437, 924, 1462
LguI GCTCTTC 2 cut(s) 1224, 1326
LmnI GCTCC 2 cut(s) 235, 854
Lsp1109I GCAGC 5 cut(s) 332, 412, 514, 774, 1156
LweI GCATC 5 cut(s) 636, 658, 1075, 1237, 1673
MaeII ACGT 4 cut(s) 166, 315, 694, 912
MaeIII GTNAC 3 cut(s) 1070, 1126, 1148
MalI GATC 4 cut(s) 123, 439, 926, 1464
MboI GATC 4 cut(s) 121, 437, 924, 1462
MboII GAAGA 9 cut(s) 191, 389, 604, 753, 1075, 1211, 1343, 1536, 1803
MfeI CAATTG 2 cut(s) 634, 1193
MflI RGATCY 1 cut(s) 437
MhlI GDGCHC 3 cut(s) 240, 859, 1001
MlyI GAGTC 3 cut(s) 485, 783, 1632
MmeI TCCRAC 2 cut(s) 1294, 1824
Mph1103I ATGCAT 2 cut(s) 1230, 1394
MroXI GAANNNNTTC 1 cut(s) 745
MseI TTAA 5 cut(s) 47, 189, 795, 1113, 1346
MslI CAYNNNNRTG 2 cut(s) 778, 1286
MspA1I CMGCKG 1 cut(s) 119
MspCI CTTAAG 1 cut(s) 188
MspI CCGG 1 cut(s) 1448
MspR9I CCNGG 3 cut(s) 734, 810, 1449
MunI CAATTG 2 cut(s) 634, 1193
MvaI CCWGG 2 cut(s) 734, 810
MvnI CGCG 1 cut(s) 63
MwoI GCNNNNNNNGC 7 cut(s) 23, 101, 431, 524, 854, 1178, 1772
NciI CCSGG 1 cut(s) 1449
NcoI CCATGG 1 cut(s) 1844
NdeI CATATG 1 cut(s) 1164
NdeII GATC 4 cut(s) 121, 437, 924, 1462
NheI GCTAGC 1 cut(s) 56
NlaIV GGNNCC 3 cut(s) 237, 513, 856
NmuCI GTSAC 2 cut(s) 1070, 1126
NsiI ATGCAT 2 cut(s) 1230, 1394
NspI RCATGY 6 cut(s) 26, 558, 828, 1396, 1807, 1858
OliI CACNNNNGTG 1 cut(s) 778
PaeI GCATGC 3 cut(s) 558, 828, 1396
PciI ACATGT 1 cut(s) 1803
PciSI GCTCTTC 2 cut(s) 1224, 1326
PcsI WCGNNNNNNNCGW 1 cut(s) 1209
PdmI GAANNNNTTC 1 cut(s) 745
PfeI GAWTC 5 cut(s) 40, 65, 962, 1198, 1715
PflFI GACNNNGTC 1 cut(s) 956
PkrI GCNGC 6 cut(s) 62, 322, 427, 529, 764, 1171
PleI GAGTC 3 cut(s) 484, 783, 1632
PpsI GAGTC 3 cut(s) 484, 783, 1632
Ppu21I YACGTR 1 cut(s) 695
PscI ACATGT 1 cut(s) 1803
PshAI GACNNNNGTC 1 cut(s) 148
PshBI ATTAAT 1 cut(s) 1346
Psp124BI GAGCTC 1 cut(s) 1001
Psp6I CCWGG 2 cut(s) 732, 808
PspGI CCWGG 2 cut(s) 732, 808
PspN4I GGNNCC 3 cut(s) 237, 513, 856
PstI CTGCAG 1 cut(s) 393
PsuI RGATCY 1 cut(s) 437
PsyI GACNNNGTC 1 cut(s) 956
PvuII CAGCTG 1 cut(s) 119
RsaI GTAC 3 cut(s) 169, 697, 1280
RsaNI GTAC 3 cut(s) 168, 696, 1279
RseI CAYNNNNRTG 2 cut(s) 778, 1286
SacI GAGCTC 1 cut(s) 1001
SapI GCTCTTC 2 cut(s) 1224, 1326
SaqAI TTAA 5 cut(s) 47, 189, 795, 1113, 1346
SatI GCNGC 6 cut(s) 61, 321, 426, 528, 763, 1170
Sau3AI GATC 4 cut(s) 121, 437, 924, 1462
SchI GAGTC 3 cut(s) 485, 783, 1632
ScrFI CCNGG 3 cut(s) 734, 810, 1449
SduI GDGCHC 3 cut(s) 240, 859, 1001
SfaNI GCATC 5 cut(s) 636, 658, 1075, 1237, 1673
SfcI CTRYAG 2 cut(s) 389, 1659
SmiMI CAYNNNNRTG 2 cut(s) 778, 1286
SmlI CTYRAG 4 cut(s) 188, 892, 1314, 1825
SmoI CTYRAG 4 cut(s) 188, 892, 1314, 1825
SphI GCATGC 3 cut(s) 558, 828, 1396
SsiI CCGC 4 cut(s) 61, 93, 498, 1689
SstI GAGCTC 1 cut(s) 1001
StyD4I CCNGG 3 cut(s) 732, 808, 1447
StyI CCWWGG 1 cut(s) 1844
TaaI ACNGT 7 cut(s) 147, 307, 543, 1267, 1342, 1420, 1587
TaiI ACGT 4 cut(s) 169, 318, 697, 915
TaqI TCGA 7 cut(s) 739, 839, 960, 1039, 1231, 1284, 1667
TaqII GACCGA 1 cut(s) 618
TatI WGTACW 1 cut(s) 1278
TauI GCSGC 1 cut(s) 63
TfiI GAWTC 5 cut(s) 40, 65, 962, 1198, 1715
Tru1I TTAA 5 cut(s) 47, 189, 795, 1113, 1346
Tru9I TTAA 5 cut(s) 47, 189, 795, 1113, 1346
TscAI CASTG 4 cut(s) 393, 415, 572, 1384
TseFI GTSAC 2 cut(s) 1070, 1126
TseI GCWGC 5 cut(s) 320, 425, 527, 762, 1169
Tsp45I GTSAC 2 cut(s) 1070, 1126
TspDTI ATGAA 7 cut(s) 315, 1044, 1113, 1377, 1385, 1561, 1642
TspGWI ACGGA 2 cut(s) 790, 1317
TspRI CASTG 4 cut(s) 393, 415, 572, 1384
Tth111I GACNNNGTC 1 cut(s) 956
Vha464I CTTAAG 1 cut(s) 188
VspI ATTAAT 1 cut(s) 1346
XapI RAATTY 2 cut(s) 940, 1732
XbaI TCTAGA 3 cut(s) 290, 442, 1504
XceI RCATGY 6 cut(s) 26, 558, 828, 1396, 1807, 1858
XmiI GTMKAC 1 cut(s) 1831
XmnI GAANNNNTTC 1 cut(s) 745
Zsp2I ATGCAT 2 cut(s) 1230, 1394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.