RchiOBHm_Chr5g0041431

Ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000157
Physical Location & Seq
Reverse (-)
36284516 .. 36286633
2118 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31987

Sequence Viewer

Length: 1836 bp
ATGTGCAACTTCATTACTCTCCATATGATTAAACCCCAAACAGGAGAAGAATACCTTAAGACATGTGTTGCCCTCTATAAGTATGCACTAAAAGGTGATTGGGAAGCCGCTGAACTAATCTTGAAAAAGGATAGAAGCAAACTTCCACGAGCTAGCATATCTAGAAGATATGAAACAGTCCTTCACGTTGCAGCAAGAACAAGCCATATTCACTTTGTGGAGAAACTGGTCAGCATGATGGAAGTAGAAGACTTGGCACTGCAGGACATAGATGGAAACACTGCCTTATGCATTGCCGCTGCGGCTGGATCTCTAGACATTGTCCAGATTTTAATACAAAAGAACAAGTCTTTGCTAACAATTAGAGGCGGTAAAGATATGGCACCGGCCTATATGGCTGCTGTGTCAGGACAGTCTGAAATGGCATGTTACCTGTACTACGAAAGTAAAAAGATGTTGGAGAAAACCGACCGAGAAAACTTTTTTTTTTCTTGCATCAACAATTGTCTATACGGTAGGTATACTAAAGTTCATCCTTCTCTATACATGCATTTGAACATTTATGGGTACAGAAAGGAATCGAAATTTTCAAAATGTCAAAATTGCATTCTTAATTTCTGTGTAAAAATTTCAGATTTGGCCTTTCAGTTGCTAGATGCTGATAAAGCATTAGCGAAGGCACGTACTAGTTCGCAGAAAAATAAAACAGCCTTGCATATCCTGGCTCGAAGACCTTCAGCATTTCGTGGCAGCCAAATTCCACCAATGTGGCCTAGACTCATTAACTCATTCCTCCCAGGTTTGAAGTTCGCATGCAAAAGAAAGTTGAGAGAAGCTAATGGAGCCCTTCAACTAGTCCACCGCCTCTGGGAAGAAATCTTGAGAGATGAGTATGATGACGTGATGAGGCTGATCAAATATCCTTCAAATTTATTCTTTGACGCAGCAGAATTAGGGAATTATGAGTTTCTGGCAGCGCTTATGAGCTCTTATCCTGATTTAGTATGGGAAACTGATGAAAAAAATCGAACCATAATCCACATCGCAGTTCTGCATCGTCACGCAAGTATCTTCAATCTAGTGCATGAAATAGGCTCAATCAAAGATGTCATCGTGACATACAAAGATGTTCAGGGTAACAACATTGTGCATATGGCTGCAAAATTAGCACCTCAAAATCAATTGAATCTCGTGTCAGGCATAGCTCTTCAAATGCATCGAGAGTTGGTATGGTTTGAGGAAGTCAAAAAGATCGTCCAACCTCAATACATCGAGATGGAAAACGACAAAGGAAAAACACCTCAAGAATTATTTACAGAAGAGCATACGGTGTTAATGCGTCAAGGAGAAAAATGGATGAAGGGCACAGCAACTTCATGCATGCTCGTTGCGACTATTATCGCAACCGTTGTGTTCTCAGTTGCATTTAGCATACCGAGTGGCACAGATGATCATATTGGAAAACCAAACCTTTTGAAACAGAAAGCCTTTCTAGACTTTACCATAGCTGACGAAGTAGCACTCTTTTCCTCTTCAACTGCAATGTTGATGTTCTTGTTCATCCTTACCTCGCGGTATGCAGAAAATGATTTCCGAAAGTCCTTACCCTTGAAGTTGATGGCAGGACTCACTAGCCTCGTCATATCTATAGCATCAATGATGATGGCTTTCAGCACTACCTTCTATCTATCTTGTCAATACGAATCAAAATGGGTTTCAAATTTTACATTTTTATTTGCATTTCTCCCAATTGCTTTGTTTGTGCTTATGAAATTTCCTCTTGTGTCCGACATGTCCTCTTCTATATTTTGCTCAAGTCTACTATTTCAGCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

611

Amino Acids

69.5

Weight (kDa)

8.74

Isoelectric Point (pI)

41.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 29 - 115 1.3e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 61 - 148 2.2e-10 Ankyrin repeats (3 copies)
PGG PF13962 450 - 563 6.1e-23 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54070 AT5G35830
fragaria_vesca FvH4_3g25070 FvH4_3g25080 FvH4_3g25081 FvH4_3g25081
malus_domestica MD03G1027500.v1.1 MD03G1146300.v1.1 MD03G1146800.v1.1 MD03G1146900.v1.1 MD03G1147100.v1.1 MD11G1165600.v1.1
prunus_persica Prupe.2G015800_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G016300_v2.0.a1 Prupe.2G016500_v2.0.a1 Prupe.6G131100_v2.0.a1 Prupe.6G131400_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1
pyrus_communis pycom03g10090 pycom03g10110 pycom11g13740 pycom11g13770
rosa_chinensis RchiOBHm_Chr1g0316371 RchiOBHm_Chr1g0316381 RchiOBHm_Chr1g0316401 RchiOBHm_Chr1g0316411 RchiOBHm_Chr1g0316571 RchiOBHm_Chr1g0316601 RchiOBHm_Chr5g0039341 RchiOBHm_Chr5g0041371 RchiOBHm_Chr5g0041401 RchiOBHm_Chr5g0041431 RchiOBHm_Chr5g0041471 RchiOBHm_Chr5g0045281 RchiOBHm_Chr5g0045291 RchiOBHm_Chr5g0045311 RchiOBHm_Chr5g0045371 RchiOBHm_Chr5g0045421 RchiOBHm_Chr5g0045431 RchiOBHm_Chr5g0045501 RchiOBHm_Chr5g0045551 RchiOBHm_Chr5g0045771 RchiOBHm_Chr5g0045811
rosa_laevigata RLG00000034057 RLG00000034058
rosa_multiflora Rmu_co8352751.1_g000001 Rmu_co8397575.1_g000001 Rmu_sc0000415.1_g000006 Rmu_sc0000493.1_g000057 Rmu_sc0000504.1_g000003 Rmu_sc0000594.1_g000019 Rmu_sc0000594.1_g000032 Rmu_sc0000594.1_g000066 Rmu_sc0001047.1_g000003 Rmu_sc0001047.1_g000011 Rmu_sc0001556.1_g000023 Rmu_sc0002584.1_g000003 Rmu_sc0002584.1_g000039 Rmu_sc0002584.1_g000070 Rmu_sc0002667.1_g000015 Rmu_sc0003677.1_g000003 Rmu_sc0003743.1_g000017 Rmu_sc0004520.1_g000004 Rmu_sc0004520.1_g000013 Rmu_sc0004520.1_g000027 Rmu_sc0005539.1_g000014 Rmu_sc0005539.1_g000032 Rmu_sc0006018.1_g000006 Rmu_sc0006737.1_g000020 Rmu_sc0007485.1_g000002 Rmu_sc0008729.1_g000002 Rmu_sc0008729.1_g000003 Rmu_sc0038822.1_g000001 Rmu_ssc0000398.1_g000025
rosa_roxburghii Rroxscaffold_1G00035090 Rroxscaffold_1G00035630 Rroxscaffold_1G00035640 Rroxscaffold_1G00035660 Rroxscaffold_1G00035680 Rroxscaffold_1G00035730 Rroxscaffold_1G00035750 Rroxscaffold_1G00035770 Rroxscaffold_1G00039150 Rroxscaffold_1G00039190 Rroxscaffold_1G00039220 Rroxscaffold_1G00039260 Rroxscaffold_4G00328910 Rroxscaffold_4G00328950 Rroxscaffold_4G00328960 Rroxscaffold_4G00330890
rosa_rugosa Rorug01G0009800 Rorug01G0010000.1 Rorug01G0010200 Rorug01G0012100 Rorug01G0023500 Rorug01G0023600 Rorug01G0023700 Rorug01G0024700 Rorug05G0192000 Rorug05G0192200 Rorug05G0222400 Rorug05G0222500 Rorug05G0222600.1 Rorug05G0222700.1 Rorug05G0222800.1 Rorug05G0222900.1 Rorug05G0223000.1 Rorug05G0223100.1 Rorug05G0223200 Rorug05G0223600 Rorug05G0226800 Rorug05G0226900
rosa_samantha Rh1CG019600 Rh1DG015000 Rh1DG015300 Rh1DG016900 Rh5CG315200 Rh5CG338700 Rh5DG277100 Rh5DG291500 Rh5DG292100 Rh5DG292200 Rh5DG292400 Rh5DG292600 Rh5DG322100 Rh5DG322200 Rh5DG323100 Rh5DG323300 Rh5DG326700 Rh5DG326900 Rh5DG327000
rosa_wichuraiana Rw0G021580 Rw1G001320 Rw1G001420 Rw1G002820 Rw1G002840 Rw1G002870 Rw5G024800 Rw5G026160 Rw5G026170 Rw5G026180 Rw5G026210 Rw5G026230 Rw5G028170 Rw5G028190 Rw5G028210 Rw5G028230 Rw5G028240 Rw5G028260 Rw5G028280 Rw5G028370 Rw5G028380 Rw5G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 382
AccI GTMKAC 2 cut(s) 521, 1819
AccII CGCG 1 cut(s) 1573
AciI CCGC 6 cut(s) 108, 297, 302, 369, 862, 1573
AclWI GGATC 1 cut(s) 316
AcsI RAATTY 6 cut(s) 584, 627, 756, 928, 1720, 1772
AcuI CTGAAG 1 cut(s) 720
AdeI CACNNNGTG 1 cut(s) 217
AfaI GTAC 3 cut(s) 437, 569, 685
AfeI AGCGCT 1 cut(s) 978
AfiI CCNNNNNNNGG 2 cut(s) 41, 868
AflII CTTAAG 1 cut(s) 56
AflIII ACRYGT 2 cut(s) 62, 1791
AhlI ACTAGT 2 cut(s) 686, 853
AjiI CACGTC 1 cut(s) 901
AjnI CCWGG 2 cut(s) 720, 796
AleI CACNNNNGTG 1 cut(s) 766
AluBI AGCT 5 cut(s) 152, 836, 987, 1205, 1508
AluI AGCT 5 cut(s) 152, 836, 987, 1205, 1508
Alw21I GWGCWC 1 cut(s) 989
AlwI GGATC 1 cut(s) 316
Aor51HI AGCGCT 1 cut(s) 978
AoxI GGCC 3 cut(s) 387, 639, 770
ApeKI GCWGC 7 cut(s) 191, 299, 398, 750, 944, 974, 1157
ApoI RAATTY 6 cut(s) 584, 627, 756, 928, 1720, 1772
ArsI GACNNNNNNTTYG 2 cut(s) 1239, 1271
Asp700I GAANNNNTTC 1 cut(s) 733
AspLEI GCGC 1 cut(s) 979
AsuHPI GGTGA 1 cut(s) 107
AsuNHI GCTAGC 1 cut(s) 152
BaeGI GKGCMC 1 cut(s) 1367
BaeI ACNNNNGTAYC 2 cut(s) 1051, 1084
BanI GGYRCC 1 cut(s) 382
BanII GRGCYC 2 cut(s) 847, 989
BauI CACGAG 2 cut(s) 147, 1190
BbsI GAAGAC 2 cut(s) 255, 736
Bbv12I GWGCWC 1 cut(s) 989
BbvI GCAGC 7 cut(s) 203, 286, 385, 762, 956, 986, 1144
BccI CCATC 5 cut(s) 232, 266, 1270, 1612, 1657
BceAI ACGGC 1 cut(s) 1816
BciT130I CCWGG 2 cut(s) 722, 798
BclI TGATCA 2 cut(s) 912, 1450
BcuI ACTAGT 2 cut(s) 686, 853
BfmI CTRYAG 2 cut(s) 260, 1647
BfoI RGCGCY 1 cut(s) 980
BfrI CTTAAG 1 cut(s) 56
BglI GCCNNNNNGGC 2 cut(s) 302, 395
Bme1390I CCNGG 2 cut(s) 722, 798
BmgBI CACGTC 1 cut(s) 901
BmiI GGNNCC 2 cut(s) 384, 844
BmrFI CCNGG 2 cut(s) 722, 798
BmsI GCATC 5 cut(s) 504, 646, 1063, 1225, 1661
BmtI GCTAGC 1 cut(s) 156
BpiI GAAGAC 2 cut(s) 255, 736
BpuEI CTTGAG 3 cut(s) 901, 1287, 1798
BsaAI YACGTR 1 cut(s) 683
BsaJI CCNNGG 1 cut(s) 796
Bsc4I CCNNNNNNNGG 2 cut(s) 41, 868
Bse118I RCCGGY 1 cut(s) 385
Bse1I ACTGG 1 cut(s) 231
Bse3DI GCAATG 2 cut(s) 291, 1548
BseBI CCWGG 2 cut(s) 722, 798
BseDI CCNNGG 1 cut(s) 796
BseGI GGATG 3 cut(s) 532, 1362, 1560
BseLI CCNNNNNNNGG 2 cut(s) 41, 868
BseMI GCAATG 2 cut(s) 291, 1548
BseMII CTCAG 1 cut(s) 1431
BseNI ACTGG 1 cut(s) 231
BseSI GKGCMC 1 cut(s) 1367
BseXI GCAGC 7 cut(s) 203, 286, 385, 762, 956, 986, 1144
Bsh1236I CGCG 1 cut(s) 1573
Bsh1285I CGRYCG 1 cut(s) 472
BshFI GGCC 3 cut(s) 389, 641, 772
BshNI GGYRCC 1 cut(s) 382
BsiEI CGRYCG 1 cut(s) 472
BsiHKAI GWGCWC 1 cut(s) 989
BsiSI CCGG 1 cut(s) 386
BslI CCNNNNNNNGG 2 cut(s) 41, 868
BsmI GAATGC 1 cut(s) 606
BsnI GGCC 3 cut(s) 389, 641, 772
Bsp1286I GDGCHC 3 cut(s) 847, 989, 1367
Bsp143I GATC 4 cut(s) 308, 912, 1251, 1450
BspACI CCGC 6 cut(s) 108, 297, 302, 369, 862, 1573
BspANI GGCC 3 cut(s) 389, 641, 772
BspCNI CTCAG 1 cut(s) 1430
BspFNI CGCG 1 cut(s) 1573
BspLI GGNNCC 2 cut(s) 384, 844
BspMAI CTGCAG 1 cut(s) 264
BspOI GCTAGC 1 cut(s) 156
BspPI GGATC 1 cut(s) 316
BspQI GCTCTTC 2 cut(s) 1212, 1314
BspT107I GGYRCC 1 cut(s) 382
BspTI CTTAAG 1 cut(s) 56
BsrDI GCAATG 2 cut(s) 291, 1548
BsrFI RCCGGY 1 cut(s) 385
BsrI ACTGG 1 cut(s) 231
BssAI RCCGGY 1 cut(s) 385
BssECI CCNNGG 1 cut(s) 796
BssMI GATC 4 cut(s) 308, 912, 1251, 1450
BssNAI GTATAC 1 cut(s) 522
BssSI CACGAG 2 cut(s) 147, 1190
Bst1107I GTATAC 1 cut(s) 522
Bst2BI CACGAG 2 cut(s) 147, 1190
Bst2UI CCWGG 2 cut(s) 722, 798
Bst4CI ACNGT 5 cut(s) 178, 414, 515, 1330, 1408
Bst6I CTCTTC 4 cut(s) 1212, 1314, 1537, 1804
BstAFI CTTAAG 1 cut(s) 56
BstBAI YACGTR 1 cut(s) 683
BstC8I GCNNGC 3 cut(s) 154, 814, 1382
BstDEI CTNAG 1 cut(s) 1417
BstF5I GGATG 3 cut(s) 532, 1362, 1560
BstFNI CGCG 1 cut(s) 1573
BstH2I RGCGCY 1 cut(s) 980
BstHHI GCGC 1 cut(s) 979
BstKTI GATC 4 cut(s) 311, 915, 1254, 1453
BstMBI GATC 4 cut(s) 308, 912, 1251, 1450
BstMCI CGRYCG 1 cut(s) 472
BstMWI GCNNNNNNNGC 5 cut(s) 302, 395, 665, 842, 1166
BstNI CCWGG 2 cut(s) 722, 798
BstNSI RCATGY 6 cut(s) 66, 429, 550, 816, 1384, 1795
BstSCI CCNGG 2 cut(s) 720, 796
BstSFI CTRYAG 2 cut(s) 260, 1647
BstSLI GKGCMC 1 cut(s) 1367
BstUI CGCG 1 cut(s) 1573
BstV1I GCAGC 7 cut(s) 203, 286, 385, 762, 956, 986, 1144
BstV2I GAAGAC 2 cut(s) 255, 736
BstX2I RGATCY 1 cut(s) 308
BstXI CCANNNNNNTGG 1 cut(s) 768
BstYI RGATCY 1 cut(s) 308
BstZ17I GTATAC 1 cut(s) 522
BsuRI GGCC 3 cut(s) 389, 641, 772
BtgZI GCGATG 1 cut(s) 1027
BtrI CACGTC 1 cut(s) 901
BtsCI GGATG 3 cut(s) 532, 1362, 1560
BtsI GCAGTG 2 cut(s) 257, 279
BtsIMutI CAGTG 2 cut(s) 257, 279
Cac8I GCNNGC 3 cut(s) 154, 814, 1382
CfoI GCGC 1 cut(s) 979
Cfr10I RCCGGY 1 cut(s) 385
CseI GACGC 2 cut(s) 950, 1328
Csp6I GTAC 3 cut(s) 436, 568, 684
CviAII CATG 9 cut(s) 63, 235, 426, 547, 813, 1085, 1377, 1381, 1792
CviQI GTAC 3 cut(s) 436, 568, 684
DdeI CTNAG 1 cut(s) 1417
DpnI GATC 4 cut(s) 310, 914, 1253, 1452
DpnII GATC 4 cut(s) 308, 912, 1251, 1450
DraIII CACNNNGTG 1 cut(s) 217
Eam1104I CTCTTC 4 cut(s) 1212, 1314, 1537, 1804
EarI CTCTTC 4 cut(s) 1212, 1314, 1537, 1804
Ecl136II GAGCTC 1 cut(s) 987
Eco24I GRGCYC 2 cut(s) 847, 989
Eco47III AGCGCT 1 cut(s) 978
Eco53kI GAGCTC 1 cut(s) 987
Eco57I CTGAAG 1 cut(s) 720
EcoICRI GAGCTC 1 cut(s) 987
EcoRII CCWGG 2 cut(s) 720, 796
EcoT22I ATGCAT 4 cut(s) 293, 552, 1218, 1382
EcoT38I GRGCYC 2 cut(s) 847, 989
FaeI CATG 9 cut(s) 66, 238, 429, 550, 816, 1088, 1380, 1384, 1795
FalI AAGNNNNNCTT 2 cut(s) 39, 71
FatI CATG 9 cut(s) 62, 234, 425, 546, 812, 1084, 1376, 1380, 1791
FauNDI CATATG 2 cut(s) 24, 1152
FbaI TGATCA 2 cut(s) 912, 1450
FblI GTMKAC 2 cut(s) 521, 1819
FokI GGATG 3 cut(s) 519, 1369, 1547
FriOI GRGCYC 2 cut(s) 847, 989
GlaI GCGC 1 cut(s) 978
HaeII RGCGCY 1 cut(s) 980
HaeIII GGCC 3 cut(s) 389, 641, 772
HapII CCGG 1 cut(s) 386
HgaI GACGC 2 cut(s) 950, 1328
HhaI GCGC 1 cut(s) 979
Hin1II CATG 9 cut(s) 66, 238, 429, 550, 816, 1088, 1380, 1384, 1795
Hin6I GCGC 1 cut(s) 977
HinP1I GCGC 1 cut(s) 977
HinfI GANTC 5 cut(s) 578, 777, 1186, 1626, 1703
HpaII CCGG 1 cut(s) 386
HphI GGTGA 1 cut(s) 107
Hpy166II GTNNAC 3 cut(s) 522, 859, 1820
Hpy188I TCNGA 4 cut(s) 418, 634, 1595, 1789
Hpy8I GTNNAC 3 cut(s) 522, 859, 1820
HpyAV CCTTC 8 cut(s) 191, 546, 670, 744, 857, 933, 1354, 1690
HpyCH4III ACNGT 5 cut(s) 178, 414, 515, 1330, 1408
HpyCH4IV ACGT 3 cut(s) 186, 682, 900
HpyF10VI GCNNNNNNNGC 5 cut(s) 302, 395, 665, 842, 1166
HpyF3I CTNAG 1 cut(s) 1417
HpySE526I ACGT 3 cut(s) 186, 682, 900
Hsp92II CATG 9 cut(s) 66, 238, 429, 550, 816, 1088, 1380, 1384, 1795
HspAI GCGC 1 cut(s) 977
Ksp22I TGATCA 2 cut(s) 912, 1450
Kzo9I GATC 4 cut(s) 308, 912, 1251, 1450
LguI GCTCTTC 2 cut(s) 1212, 1314
LmnI GCTCC 1 cut(s) 842
Lsp1109I GCAGC 7 cut(s) 203, 286, 385, 762, 956, 986, 1144
LweI GCATC 5 cut(s) 504, 646, 1063, 1225, 1661
MaeII ACGT 3 cut(s) 186, 682, 900
MaeIII GTNAC 4 cut(s) 428, 1058, 1114, 1136
MalI GATC 4 cut(s) 310, 914, 1253, 1452
MboI GATC 4 cut(s) 308, 912, 1251, 1450
MfeI CAATTG 3 cut(s) 502, 1181, 1749
MflI RGATCY 1 cut(s) 308
MhlI GDGCHC 3 cut(s) 847, 989, 1367
MlyI GAGTC 2 cut(s) 771, 1620
MmeI TCCRAC 3 cut(s) 438, 1282, 1812
Mph1103I ATGCAT 4 cut(s) 293, 552, 1218, 1382
MroXI GAANNNNTTC 1 cut(s) 733
MseI TTAA 6 cut(s) 30, 57, 332, 612, 783, 1334
MslI CAYNNNNRTG 2 cut(s) 766, 1274
MspA1I CMGCKG 2 cut(s) 110, 299
MspCI CTTAAG 1 cut(s) 56
MspI CCGG 1 cut(s) 386
MspR9I CCNGG 2 cut(s) 722, 798
MunI CAATTG 3 cut(s) 502, 1181, 1749
Mva1269I GAATGC 1 cut(s) 606
MvaI CCWGG 2 cut(s) 722, 798
MvnI CGCG 1 cut(s) 1573
MwoI GCNNNNNNNGC 5 cut(s) 302, 395, 665, 842, 1166
NdeI CATATG 2 cut(s) 24, 1152
NdeII GATC 4 cut(s) 308, 912, 1251, 1450
NheI GCTAGC 1 cut(s) 152
NlaIII CATG 9 cut(s) 66, 238, 429, 550, 816, 1088, 1380, 1384, 1795
NlaIV GGNNCC 2 cut(s) 384, 844
NmuCI GTSAC 2 cut(s) 1058, 1114
NsiI ATGCAT 4 cut(s) 293, 552, 1218, 1382
NspI RCATGY 6 cut(s) 66, 429, 550, 816, 1384, 1795
OliI CACNNNNGTG 1 cut(s) 766
PaeI GCATGC 2 cut(s) 816, 1384
PciI ACATGT 2 cut(s) 62, 1791
PciSI GCTCTTC 2 cut(s) 1212, 1314
PctI GAATGC 1 cut(s) 606
PdmI GAANNNNTTC 1 cut(s) 733
PfeI GAWTC 3 cut(s) 578, 1186, 1703
PflFI GACNNNGTC 1 cut(s) 320
PleI GAGTC 2 cut(s) 771, 1620
PpsI GAGTC 2 cut(s) 771, 1620
Ppu21I YACGTR 1 cut(s) 683
PscI ACATGT 2 cut(s) 62, 1791
Psp124BI GAGCTC 1 cut(s) 989
Psp6I CCWGG 2 cut(s) 720, 796
PspGI CCWGG 2 cut(s) 720, 796
PspN4I GGNNCC 2 cut(s) 384, 844
PstI CTGCAG 1 cut(s) 264
PsuI RGATCY 1 cut(s) 308
PsyI GACNNNGTC 1 cut(s) 320
RsaI GTAC 3 cut(s) 437, 569, 685
RsaNI GTAC 3 cut(s) 436, 568, 684
RseI CAYNNNNRTG 2 cut(s) 766, 1274
SacI GAGCTC 1 cut(s) 989
SapI GCTCTTC 2 cut(s) 1212, 1314
SaqAI TTAA 6 cut(s) 30, 57, 332, 612, 783, 1334
Sau3AI GATC 4 cut(s) 308, 912, 1251, 1450
SchI GAGTC 2 cut(s) 771, 1620
ScrFI CCNGG 2 cut(s) 722, 798
SduI GDGCHC 3 cut(s) 847, 989, 1367
SfaNI GCATC 5 cut(s) 504, 646, 1063, 1225, 1661
SfcI CTRYAG 2 cut(s) 260, 1647
SmiMI CAYNNNNRTG 2 cut(s) 766, 1274
SmlI CTYRAG 4 cut(s) 56, 880, 1302, 1813
SmoI CTYRAG 4 cut(s) 56, 880, 1302, 1813
SpeI ACTAGT 2 cut(s) 686, 853
SphI GCATGC 2 cut(s) 816, 1384
SsiI CCGC 6 cut(s) 108, 297, 302, 369, 862, 1573
SstI GAGCTC 1 cut(s) 989
StyD4I CCNGG 2 cut(s) 720, 796
TaaI ACNGT 5 cut(s) 178, 414, 515, 1330, 1408
TaiI ACGT 3 cut(s) 189, 685, 903
TaqI TCGA 5 cut(s) 581, 727, 1027, 1219, 1272
TaqII GACCGA 1 cut(s) 486
TatI WGTACW 1 cut(s) 435
TauI GCSGC 3 cut(s) 110, 299, 305
TfiI GAWTC 3 cut(s) 578, 1186, 1703
Tru1I TTAA 6 cut(s) 30, 57, 332, 612, 783, 1334
Tru9I TTAA 6 cut(s) 30, 57, 332, 612, 783, 1334
TscAI CASTG 2 cut(s) 264, 286
TseFI GTSAC 2 cut(s) 1058, 1114
TseI GCWGC 7 cut(s) 191, 299, 398, 750, 944, 974, 1157
Tsp45I GTSAC 2 cut(s) 1058, 1114
TspDTI ATGAA 8 cut(s) 186, 521, 1032, 1101, 1365, 1373, 1549, 1784
TspRI CASTG 2 cut(s) 264, 286
Tth111I GACNNNGTC 1 cut(s) 320
Vha464I CTTAAG 1 cut(s) 56
XapI RAATTY 6 cut(s) 584, 627, 756, 928, 1720, 1772
XbaI TCTAGA 3 cut(s) 161, 313, 1492
XceI RCATGY 6 cut(s) 66, 429, 550, 816, 1384, 1795
XmiI GTMKAC 2 cut(s) 521, 1819
XmnI GAANNNNTTC 1 cut(s) 733
Zsp2I ATGCAT 4 cut(s) 293, 552, 1218, 1382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.