Rh5DG327000

Ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000157
Physical Location & Seq
Reverse (-)
42944255 .. 42945493
1239 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG327000.1

Sequence Viewer

Length: 750 bp
ATGGCTACTGGTAATGGAGATCCAATGGCAAGTGCACATACTGCCATTAACATTCACTCGATACATTCGCATCGTTTTCTAGAAAACAACAATAGAGAGCTATACCTTAATACATGTGTGCCACTCTCTAAGTATGCACTAAAAGGCAATTGGGAAGCTGCTGAACGAATCTTGAAGGAGGATCCGAGTCTTTTGACTTCTAGCATAACAAGAGGGGGGGACACTGTCCTTCACATTGCAGCAGGAGCAAGACATGTTCGCTTTGTGGAGAAGCTGGTCGAAATGATGGAAGAACTGAATGGAAACGAGACATGTTCACAAAACTTGGCACTGCAAGACATGAATGGAAACACTGCCTTCATCATTGCTGCTGCAGCTGGAAGTATTGAAATTGCAGAGATTTTGATCAAGAAGAATAAGGATTTGCAAATAATTCGAGGTGGTGAAGGAATGACACCACTCTATATGGCTGCCTTGTTGGGACAGTCTGTAATGGCAGATAACCTGTACTCTAGAACCAAGGAAATGTTGGAGGAAGTCGACCGCCAAGCGTTGTTTTTTACTTGTATCGATAATGGTCTGTACGATCTAGCTTTGAAGATTCTAAGAAGTGACAGAGCATTAGCTAAGGCTTGTAATGCAAAAGAGGAAACAGCTTTGCATATCTTGGCTAGAACGCCTTCAGACTTCACCAGCCAAAGTCCAGGAACGTGGAGTAGACTCATCAACTCATGTGAGTCGGTTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

249

Amino Acids

27.35

Weight (kDa)

5.67

Isoelectric Point (pI)

43.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 46 - 143 2.6e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 108 - 170 3.3e-08 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54070 AT5G35830
fragaria_vesca FvH4_3g25070 FvH4_3g25080 FvH4_3g25081 FvH4_3g25081
malus_domestica MD03G1027500.v1.1 MD03G1146300.v1.1 MD03G1146800.v1.1 MD03G1146900.v1.1 MD03G1147100.v1.1 MD11G1165600.v1.1
prunus_persica Prupe.2G015800_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G016300_v2.0.a1 Prupe.2G016500_v2.0.a1 Prupe.6G131100_v2.0.a1 Prupe.6G131400_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1
pyrus_communis pycom03g10090 pycom03g10110 pycom11g13740 pycom11g13770
rosa_chinensis RchiOBHm_Chr1g0316371 RchiOBHm_Chr1g0316381 RchiOBHm_Chr1g0316401 RchiOBHm_Chr1g0316411 RchiOBHm_Chr1g0316571 RchiOBHm_Chr1g0316601 RchiOBHm_Chr5g0039341 RchiOBHm_Chr5g0041371 RchiOBHm_Chr5g0041401 RchiOBHm_Chr5g0041431 RchiOBHm_Chr5g0041471 RchiOBHm_Chr5g0045281 RchiOBHm_Chr5g0045291 RchiOBHm_Chr5g0045311 RchiOBHm_Chr5g0045371 RchiOBHm_Chr5g0045421 RchiOBHm_Chr5g0045431 RchiOBHm_Chr5g0045501 RchiOBHm_Chr5g0045551 RchiOBHm_Chr5g0045771 RchiOBHm_Chr5g0045811
rosa_laevigata RLG00000034057 RLG00000034058
rosa_multiflora Rmu_co8352751.1_g000001 Rmu_co8397575.1_g000001 Rmu_sc0000415.1_g000006 Rmu_sc0000493.1_g000057 Rmu_sc0000504.1_g000003 Rmu_sc0000594.1_g000019 Rmu_sc0000594.1_g000032 Rmu_sc0000594.1_g000066 Rmu_sc0001047.1_g000003 Rmu_sc0001047.1_g000011 Rmu_sc0001556.1_g000023 Rmu_sc0002584.1_g000003 Rmu_sc0002584.1_g000039 Rmu_sc0002584.1_g000070 Rmu_sc0002667.1_g000015 Rmu_sc0003677.1_g000003 Rmu_sc0003743.1_g000017 Rmu_sc0004520.1_g000004 Rmu_sc0004520.1_g000013 Rmu_sc0004520.1_g000027 Rmu_sc0005539.1_g000014 Rmu_sc0005539.1_g000032 Rmu_sc0006018.1_g000006 Rmu_sc0006737.1_g000020 Rmu_sc0007485.1_g000002 Rmu_sc0008729.1_g000002 Rmu_sc0008729.1_g000003 Rmu_sc0038822.1_g000001 Rmu_ssc0000398.1_g000025
rosa_roxburghii Rroxscaffold_1G00035090 Rroxscaffold_1G00035630 Rroxscaffold_1G00035640 Rroxscaffold_1G00035660 Rroxscaffold_1G00035680 Rroxscaffold_1G00035730 Rroxscaffold_1G00035750 Rroxscaffold_1G00035770 Rroxscaffold_1G00039150 Rroxscaffold_1G00039190 Rroxscaffold_1G00039220 Rroxscaffold_1G00039260 Rroxscaffold_4G00328910 Rroxscaffold_4G00328950 Rroxscaffold_4G00328960 Rroxscaffold_4G00330890
rosa_rugosa Rorug01G0009800 Rorug01G0010000.1 Rorug01G0010200 Rorug01G0012100 Rorug01G0023500 Rorug01G0023600 Rorug01G0023700 Rorug01G0024700 Rorug05G0192000 Rorug05G0192200 Rorug05G0222400 Rorug05G0222500 Rorug05G0222600.1 Rorug05G0222700.1 Rorug05G0222800.1 Rorug05G0222900.1 Rorug05G0223000.1 Rorug05G0223100.1 Rorug05G0223200 Rorug05G0223600 Rorug05G0226800 Rorug05G0226900
rosa_samantha Rh1CG019600 Rh1DG015000 Rh1DG015300 Rh1DG016900 Rh5CG315200 Rh5CG338700 Rh5DG277100 Rh5DG291500 Rh5DG292100 Rh5DG292200 Rh5DG292400 Rh5DG292600 Rh5DG322100 Rh5DG322200 Rh5DG323100 Rh5DG323300 Rh5DG326700 Rh5DG326900 Rh5DG327000
rosa_wichuraiana Rw0G021580 Rw1G001320 Rw1G001420 Rw1G002820 Rw1G002840 Rw1G002870 Rw5G024800 Rw5G026160 Rw5G026170 Rw5G026180 Rw5G026210 Rw5G026230 Rw5G028170 Rw5G028190 Rw5G028210 Rw5G028230 Rw5G028240 Rw5G028260 Rw5G028280 Rw5G028370 Rw5G028380 Rw5G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 540, 718
AciI CCGC 1 cut(s) 544
AclWI GGATC 3 cut(s) 14, 176, 189
AcuI CTGAAG 1 cut(s) 666
AfaI GTAC 2 cut(s) 509, 584
AflIII ACRYGT 3 cut(s) 113, 253, 311
AgsI TTSAA 3 cut(s) 175, 389, 598
AjnI CCWGG 1 cut(s) 703
AluBI AGCT 7 cut(s) 100, 158, 274, 377, 593, 626, 656
AluI AGCT 7 cut(s) 100, 158, 274, 377, 593, 626, 656
Alw21I GWGCWC 1 cut(s) 37
Alw26I GTCTC 1 cut(s) 302
Alw44I GTGCAC 1 cut(s) 33
AlwI GGATC 3 cut(s) 14, 176, 189
ApaLI GTGCAC 1 cut(s) 33
ApeKI GCWGC 6 cut(s) 158, 239, 368, 371, 374, 470
Asp700I GAANNNNTTC 1 cut(s) 679
AsuHPI GGTGA 2 cut(s) 455, 682
BaeGI GKGCMC 1 cut(s) 37
BamHI GGATCC 1 cut(s) 181
Bbv12I GWGCWC 1 cut(s) 37
BbvI GCAGC 6 cut(s) 145, 251, 355, 358, 386, 457
BccI CCATC 1 cut(s) 280
BcgI CGANNNNNNTGC 2 cut(s) 416, 450
BciT130I CCWGG 1 cut(s) 705
BclI TGATCA 1 cut(s) 405
BcoDI GTCTC 1 cut(s) 302
BfaI CTAG 5 cut(s) 80, 201, 513, 590, 672
BfmI CTRYAG 1 cut(s) 372
BisI GCNGC 6 cut(s) 159, 240, 369, 372, 375, 471
BlsI GCNGC 6 cut(s) 160, 241, 370, 373, 376, 472
Bme1390I CCNGG 1 cut(s) 705
BmiI GGNNCC 1 cut(s) 183
BmrFI CCNGG 1 cut(s) 705
BmsI GCATC 1 cut(s) 79
Bpu10I CCTNAGC 1 cut(s) 627
Bsa29I ATCGAT 1 cut(s) 570
BsaBI GATNNNNATC 1 cut(s) 404
BsaJI CCNNGG 1 cut(s) 519
Bse1I ACTGG 1 cut(s) 13
Bse3DI GCAATG 2 cut(s) 234, 363
Bse8I GATNNNNATC 1 cut(s) 404
BseBI CCWGG 1 cut(s) 705
BseCI ATCGAT 1 cut(s) 570
BseDI CCNNGG 1 cut(s) 519
BseJI GATNNNNATC 1 cut(s) 404
BseMI GCAATG 2 cut(s) 234, 363
BseNI ACTGG 1 cut(s) 13
BseSI GKGCMC 1 cut(s) 37
BseXI GCAGC 6 cut(s) 145, 251, 355, 358, 386, 457
Bsh1285I CGRYCG 1 cut(s) 544
BshVI ATCGAT 1 cut(s) 570
BsiEI CGRYCG 1 cut(s) 544
BsiHKAI GWGCWC 1 cut(s) 37
BslFI GGGAC 2 cut(s) 233, 495
BsmAI GTCTC 1 cut(s) 302
BsmFI GGGAC 2 cut(s) 233, 495
Bsp1286I GDGCHC 1 cut(s) 37
Bsp143I GATC 4 cut(s) 19, 181, 405, 586
BspACI CCGC 1 cut(s) 544
BspDI ATCGAT 1 cut(s) 570
BspLI GGNNCC 1 cut(s) 183
BspMAI CTGCAG 1 cut(s) 376
BspPI GGATC 3 cut(s) 14, 176, 189
BsrDI GCAATG 2 cut(s) 234, 363
BsrI ACTGG 1 cut(s) 13
BssECI CCNNGG 1 cut(s) 519
BssMI GATC 4 cut(s) 19, 181, 405, 586
BssT1I CCWWGG 1 cut(s) 519
Bst2UI CCWGG 1 cut(s) 705
Bst4CI ACNGT 2 cut(s) 226, 486
BstAPI GCANNNNNTGC 1 cut(s) 41
BstDEI CTNAG 3 cut(s) 129, 605, 627
BstKTI GATC 4 cut(s) 22, 184, 408, 589
BstMAI GTCTC 1 cut(s) 302
BstMBI GATC 4 cut(s) 19, 181, 405, 586
BstMCI CGRYCG 1 cut(s) 544
BstMWI GCNNNNNNNGC 4 cut(s) 41, 245, 374, 638
BstNI CCWGG 1 cut(s) 705
BstNSI RCATGY 3 cut(s) 117, 257, 315
BstSCI CCNGG 1 cut(s) 703
BstSFI CTRYAG 1 cut(s) 372
BstSLI GKGCMC 1 cut(s) 37
BstV1I GCAGC 6 cut(s) 145, 251, 355, 358, 386, 457
BstX2I RGATCY 2 cut(s) 19, 181
BstXI CCANNNNNNTGG 1 cut(s) 711
BstYI RGATCY 2 cut(s) 19, 181
Bsu15I ATCGAT 1 cut(s) 570
BsuTUI ATCGAT 1 cut(s) 570
BtsI GCAGTG 2 cut(s) 329, 351
BtsIMutI CAGTG 3 cut(s) 222, 329, 351
ClaI ATCGAT 1 cut(s) 570
Csp6I GTAC 2 cut(s) 508, 583
CviAII CATG 5 cut(s) 114, 254, 312, 340, 732
CviQI GTAC 2 cut(s) 508, 583
DdeI CTNAG 3 cut(s) 129, 605, 627
DpnI GATC 4 cut(s) 21, 183, 407, 588
DpnII GATC 4 cut(s) 19, 181, 405, 586
Eco130I CCWWGG 1 cut(s) 519
Eco57I CTGAAG 1 cut(s) 666
EcoRII CCWGG 1 cut(s) 703
EcoT14I CCWWGG 1 cut(s) 519
ErhI CCWWGG 1 cut(s) 519
FaeI CATG 5 cut(s) 117, 257, 315, 343, 735
FaqI GGGAC 2 cut(s) 233, 495
FatI CATG 5 cut(s) 113, 253, 311, 339, 731
FbaI TGATCA 1 cut(s) 405
FblI GTMKAC 2 cut(s) 540, 718
Fnu4HI GCNGC 6 cut(s) 159, 240, 369, 372, 375, 471
Fsp4HI GCNGC 6 cut(s) 159, 240, 369, 372, 375, 471
FspBI CTAG 5 cut(s) 80, 201, 513, 590, 672
GluI GCNGC 6 cut(s) 159, 240, 369, 372, 375, 471
Hin1II CATG 5 cut(s) 117, 257, 315, 343, 735
HincII GTYRAC 1 cut(s) 541
HindII GTYRAC 1 cut(s) 541
HinfI GANTC 5 cut(s) 168, 187, 601, 720, 737
HphI GGTGA 2 cut(s) 455, 682
Hpy166II GTNNAC 4 cut(s) 35, 317, 541, 719
Hpy188I TCNGA 2 cut(s) 186, 685
Hpy188III TCNNGA 4 cut(s) 80, 172, 409, 513
Hpy8I GTNNAC 4 cut(s) 35, 317, 541, 719
HpyAV CCTTC 5 cut(s) 169, 239, 367, 440, 690
HpyCH4III ACNGT 2 cut(s) 226, 486
HpyCH4IV ACGT 1 cut(s) 710
HpyCH4V TGCA 9 cut(s) 35, 137, 239, 334, 374, 395, 427, 641, 661
HpyF10VI GCNNNNNNNGC 4 cut(s) 41, 245, 374, 638
HpyF3I CTNAG 3 cut(s) 129, 605, 627
HpySE526I ACGT 1 cut(s) 710
Hsp92II CATG 5 cut(s) 117, 257, 315, 343, 735
Ksp22I TGATCA 1 cut(s) 405
Kzo9I GATC 4 cut(s) 19, 181, 405, 586
LmnI GCTCC 1 cut(s) 245
LpnPI CCDG 7 cut(s) 228, 260, 363, 518, 690, 706, 717
Lsp1109I GCAGC 6 cut(s) 145, 251, 355, 358, 386, 457
LweI GCATC 1 cut(s) 79
MaeI CTAG 5 cut(s) 80, 201, 513, 590, 672
MaeII ACGT 1 cut(s) 710
MaeIII GTNAC 1 cut(s) 611
MalI GATC 4 cut(s) 21, 183, 407, 588
MboI GATC 4 cut(s) 19, 181, 405, 586
MboII GAAGA 3 cut(s) 302, 424, 610
MfeI CAATTG 1 cut(s) 148
MflI RGATCY 2 cut(s) 19, 181
MhlI GDGCHC 1 cut(s) 37
MluCI AATT 3 cut(s) 148, 390, 432
MlyI GAGTC 3 cut(s) 196, 714, 746
MmeI TCCRAC 1 cut(s) 510
MnlI CCTC 5 cut(s) 172, 206, 431, 526, 640
MroXI GAANNNNTTC 1 cut(s) 679
MseI TTAA 3 cut(s) 48, 108, 748
MspA1I CMGCKG 1 cut(s) 377
MspR9I CCNGG 1 cut(s) 705
MunI CAATTG 1 cut(s) 148
MvaI CCWGG 1 cut(s) 705
MwoI GCNNNNNNNGC 4 cut(s) 41, 245, 374, 638
NdeII GATC 4 cut(s) 19, 181, 405, 586
NlaIII CATG 5 cut(s) 117, 257, 315, 343, 735
NlaIV GGNNCC 1 cut(s) 183
NmuCI GTSAC 1 cut(s) 611
NspI RCATGY 3 cut(s) 117, 257, 315
PciI ACATGT 3 cut(s) 113, 253, 311
PdmI GAANNNNTTC 1 cut(s) 679
PfeI GAWTC 2 cut(s) 168, 601
PflFI GACNNNGTC 1 cut(s) 224
PfoI TCCNGGA 1 cut(s) 703
PkrI GCNGC 6 cut(s) 160, 241, 370, 373, 376, 472
PleI GAGTC 3 cut(s) 195, 714, 745
PpsI GAGTC 3 cut(s) 195, 714, 745
PscI ACATGT 3 cut(s) 113, 253, 311
Psp6I CCWGG 1 cut(s) 703
PspGI CCWGG 1 cut(s) 703
PspN4I GGNNCC 1 cut(s) 183
PstI CTGCAG 1 cut(s) 376
PsuI RGATCY 2 cut(s) 19, 181
PsyI GACNNNGTC 1 cut(s) 224
PvuII CAGCTG 1 cut(s) 377
RsaI GTAC 2 cut(s) 509, 584
RsaNI GTAC 2 cut(s) 508, 583
SalI GTCGAC 1 cut(s) 539
SaqAI TTAA 3 cut(s) 48, 108, 748
SatI GCNGC 6 cut(s) 159, 240, 369, 372, 375, 471
Sau3AI GATC 4 cut(s) 19, 181, 405, 586
SchI GAGTC 3 cut(s) 196, 714, 746
ScrFI CCNGG 1 cut(s) 705
SduI GDGCHC 1 cut(s) 37
SfaNI GCATC 1 cut(s) 79
SfcI CTRYAG 1 cut(s) 372
Sse9I AATT 3 cut(s) 148, 390, 432
SsiI CCGC 1 cut(s) 544
SspMI CTAG 5 cut(s) 80, 201, 513, 590, 672
StyD4I CCNGG 1 cut(s) 703
StyI CCWWGG 1 cut(s) 519
TaaI ACNGT 2 cut(s) 226, 486
TaiI ACGT 1 cut(s) 713
TaqI TCGA 5 cut(s) 59, 279, 436, 540, 570
TasI AATT 3 cut(s) 148, 390, 432
TatI WGTACW 1 cut(s) 507
TfiI GAWTC 2 cut(s) 168, 601
Tru1I TTAA 3 cut(s) 48, 108, 748
Tru9I TTAA 3 cut(s) 48, 108, 748
TscAI CASTG 3 cut(s) 229, 336, 358
TseFI GTSAC 1 cut(s) 611
TseI GCWGC 6 cut(s) 158, 239, 368, 371, 374, 470
Tsp45I GTSAC 1 cut(s) 611
TspDTI ATGAA 2 cut(s) 349, 356
TspRI CASTG 3 cut(s) 229, 336, 358
Tth111I GACNNNGTC 1 cut(s) 224
VneI GTGCAC 1 cut(s) 33
XbaI TCTAGA 2 cut(s) 79, 512
XceI RCATGY 3 cut(s) 117, 257, 315
XcmI CCANNNNNNNNNTGG 1 cut(s) 526
XmiI GTMKAC 2 cut(s) 540, 718
XmnI GAANNNNTTC 1 cut(s) 679
XspI CTAG 5 cut(s) 80, 201, 513, 590, 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.