Rh5DG322200

Ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000157
Physical Location & Seq
Reverse (-)
41876085 .. 41878176
2092 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG322200.1

Sequence Viewer

Length: 804 bp
ATGGCTACCCCACAAGCACAACATATATCACCTGCCGGGATTCAATTAACCACGGCTAGCGGGAATTCAGCAACTGGGATAGATAGTCAATCAGCGGACATTGGCACCGACATTTCGGCTGATTCGGCTCAACCTACCAGACCTTTGCCTTATCTACTAGAACATATAAATAGAGAAGAATACCTTAAGACATGTGTTGCCCTCTATAAGTATGCACTAAAGGGCGATTGGGAAGCTGCGGAACGAATCTTGAAAAAGGATACGAAACTTCTGAGAGCAAGCATATCTAGAGGATATGAAACAGTCCTTCACGTTGCAGCAGGGGCAAGACGTATTCATTTTGTGGAGAAACTGATCAGCATGATGAACGTAGAGGATTTGGCACTGCAGGACGTAAATGGAAACACTGCCTTGTGTATTGCCGCTGCGGCTGGATCTCTAGACATTGTCCAGATTTTGATACAAAAGAATAAGTCTTTGCTATCGATTCGAGGCGGTAAAGATATGACACCAGCCTACATGGCTGTTGTGTTGGGACAGTCAGAAATGGCATGGCACCTGTACGCTGAAAGTAACACGATGTTGGAGAAAACAGACCGCGAAAACTTGTTTTTTTCTTGCATCAACAATTGTTTGTATGATTTGGCCTTACCGTTGCTAAATGATGATAGAACATTAGCGACTGCACGTACTAAATCGCAGAAATATAAAACAGCCTTGCATATATTGGCTAGAAGGCCTTCAGCATTTGGTGGCAGCCAAAGTCCAGGAATGTGGAGTAGACTTACTAACTCATGTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

267

Amino Acids

29.25

Weight (kDa)

7.65

Isoelectric Point (pI)

48.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 72 - 157 9.8e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 103 - 190 2.7e-10 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54070 AT5G35830
fragaria_vesca FvH4_3g25070 FvH4_3g25080 FvH4_3g25081 FvH4_3g25081
malus_domestica MD03G1027500.v1.1 MD03G1146300.v1.1 MD03G1146800.v1.1 MD03G1146900.v1.1 MD03G1147100.v1.1 MD11G1165600.v1.1
prunus_persica Prupe.2G015800_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G016300_v2.0.a1 Prupe.2G016500_v2.0.a1 Prupe.6G131100_v2.0.a1 Prupe.6G131400_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1
pyrus_communis pycom03g10090 pycom03g10110 pycom11g13740 pycom11g13770
rosa_chinensis RchiOBHm_Chr1g0316371 RchiOBHm_Chr1g0316381 RchiOBHm_Chr1g0316401 RchiOBHm_Chr1g0316411 RchiOBHm_Chr1g0316571 RchiOBHm_Chr1g0316601 RchiOBHm_Chr5g0039341 RchiOBHm_Chr5g0041371 RchiOBHm_Chr5g0041401 RchiOBHm_Chr5g0041431 RchiOBHm_Chr5g0041471 RchiOBHm_Chr5g0045281 RchiOBHm_Chr5g0045291 RchiOBHm_Chr5g0045311 RchiOBHm_Chr5g0045371 RchiOBHm_Chr5g0045421 RchiOBHm_Chr5g0045431 RchiOBHm_Chr5g0045501 RchiOBHm_Chr5g0045551 RchiOBHm_Chr5g0045771 RchiOBHm_Chr5g0045811
rosa_laevigata RLG00000034057 RLG00000034058
rosa_multiflora Rmu_co8352751.1_g000001 Rmu_co8397575.1_g000001 Rmu_sc0000415.1_g000006 Rmu_sc0000493.1_g000057 Rmu_sc0000504.1_g000003 Rmu_sc0000594.1_g000019 Rmu_sc0000594.1_g000032 Rmu_sc0000594.1_g000066 Rmu_sc0001047.1_g000003 Rmu_sc0001047.1_g000011 Rmu_sc0001556.1_g000023 Rmu_sc0002584.1_g000003 Rmu_sc0002584.1_g000039 Rmu_sc0002584.1_g000070 Rmu_sc0002667.1_g000015 Rmu_sc0003677.1_g000003 Rmu_sc0003743.1_g000017 Rmu_sc0004520.1_g000004 Rmu_sc0004520.1_g000013 Rmu_sc0004520.1_g000027 Rmu_sc0005539.1_g000014 Rmu_sc0005539.1_g000032 Rmu_sc0006018.1_g000006 Rmu_sc0006737.1_g000020 Rmu_sc0007485.1_g000002 Rmu_sc0008729.1_g000002 Rmu_sc0008729.1_g000003 Rmu_sc0038822.1_g000001 Rmu_ssc0000398.1_g000025
rosa_roxburghii Rroxscaffold_1G00035090 Rroxscaffold_1G00035630 Rroxscaffold_1G00035640 Rroxscaffold_1G00035660 Rroxscaffold_1G00035680 Rroxscaffold_1G00035730 Rroxscaffold_1G00035750 Rroxscaffold_1G00035770 Rroxscaffold_1G00039150 Rroxscaffold_1G00039190 Rroxscaffold_1G00039220 Rroxscaffold_1G00039260 Rroxscaffold_4G00328910 Rroxscaffold_4G00328950 Rroxscaffold_4G00328960 Rroxscaffold_4G00330890
rosa_rugosa Rorug01G0009800 Rorug01G0010000.1 Rorug01G0010200 Rorug01G0012100 Rorug01G0023500 Rorug01G0023600 Rorug01G0023700 Rorug01G0024700 Rorug05G0192000 Rorug05G0192200 Rorug05G0222400 Rorug05G0222500 Rorug05G0222600.1 Rorug05G0222700.1 Rorug05G0222800.1 Rorug05G0222900.1 Rorug05G0223000.1 Rorug05G0223100.1 Rorug05G0223200 Rorug05G0223600 Rorug05G0226800 Rorug05G0226900
rosa_samantha Rh1CG019600 Rh1DG015000 Rh1DG015300 Rh1DG016900 Rh5CG315200 Rh5CG338700 Rh5DG277100 Rh5DG291500 Rh5DG292100 Rh5DG292200 Rh5DG292400 Rh5DG292600 Rh5DG322100 Rh5DG322200 Rh5DG323100 Rh5DG323300 Rh5DG326700 Rh5DG326900 Rh5DG327000
rosa_wichuraiana Rw0G021580 Rw1G001320 Rw1G001420 Rw1G002820 Rw1G002840 Rw1G002870 Rw5G024800 Rw5G026160 Rw5G026170 Rw5G026180 Rw5G026210 Rw5G026230 Rw5G028170 Rw5G028190 Rw5G028210 Rw5G028230 Rw5G028240 Rw5G028260 Rw5G028280 Rw5G028370 Rw5G028380 Rw5G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 40
Acc36I ACCTGC 1 cut(s) 40
AccB1I GGYRCC 2 cut(s) 104, 555
AccI GTMKAC 1 cut(s) 781
AccII CGCG 1 cut(s) 600
AciI CCGC 7 cut(s) 60, 95, 239, 423, 428, 495, 598
AclWI GGATC 1 cut(s) 442
AcsI RAATTY 1 cut(s) 64
AcuI CTGAAG 1 cut(s) 726
AfaI GTAC 2 cut(s) 563, 691
AflII CTTAAG 1 cut(s) 185
AflIII ACRYGT 1 cut(s) 191
AgsI TTSAA 2 cut(s) 44, 253
AjnI CCWGG 1 cut(s) 766
AluBI AGCT 1 cut(s) 236
AluI AGCT 1 cut(s) 236
AlwI GGATC 1 cut(s) 442
AlwNI CAGNNNCTG 1 cut(s) 74
AoxI GGCC 2 cut(s) 645, 737
ApeKI GCWGC 4 cut(s) 236, 317, 425, 756
ApoI RAATTY 1 cut(s) 64
Asp700I GAANNNNTTC 1 cut(s) 739
AsuC2I CCSGG 1 cut(s) 37
AsuHPI GGTGA 1 cut(s) 21
AsuNHI GCTAGC 1 cut(s) 56
BanI GGYRCC 2 cut(s) 104, 555
BbvI GCAGC 4 cut(s) 223, 329, 412, 768
BceAI ACGGC 1 cut(s) 69
BciT130I CCWGG 1 cut(s) 768
BciVI GTATCC 1 cut(s) 253
BclI TGATCA 1 cut(s) 354
BcnI CCSGG 1 cut(s) 37
BfaI CTAG 5 cut(s) 57, 158, 288, 440, 732
BfmI CTRYAG 1 cut(s) 386
BfrI CTTAAG 1 cut(s) 185
BfuAI ACCTGC 1 cut(s) 40
BfuI GTATCC 1 cut(s) 253
BglI GCCNNNNNGGC 2 cut(s) 428, 521
BisI GCNGC 6 cut(s) 237, 318, 423, 426, 429, 757
BlsI GCNGC 6 cut(s) 238, 319, 424, 427, 430, 758
Bme1390I CCNGG 2 cut(s) 37, 768
BmiI GGNNCC 2 cut(s) 106, 557
BmrFI CCNGG 2 cut(s) 37, 768
BmrI ACTGGG 1 cut(s) 84
BmsI GCATC 1 cut(s) 630
BmtI GCTAGC 1 cut(s) 60
BmuI ACTGGG 1 cut(s) 84
BpuMI CCSGG 1 cut(s) 37
Bsa29I ATCGAT 1 cut(s) 485
BsaAI YACGTR 1 cut(s) 689
BsaJI CCNNGG 1 cut(s) 51
Bse1I ACTGG 1 cut(s) 79
BseBI CCWGG 1 cut(s) 768
BseCI ATCGAT 1 cut(s) 485
BseDI CCNNGG 1 cut(s) 51
BseMII CTCAG 1 cut(s) 263
BseNI ACTGG 1 cut(s) 79
BseXI GCAGC 4 cut(s) 223, 329, 412, 768
BsgI GTGCAG 1 cut(s) 669
Bsh1236I CGCG 1 cut(s) 600
BshFI GGCC 2 cut(s) 647, 739
BshNI GGYRCC 2 cut(s) 104, 555
BshVI ATCGAT 1 cut(s) 485
BsiSI CCGG 1 cut(s) 36
BslFI GGGAC 1 cut(s) 549
BsmFI GGGAC 1 cut(s) 549
BsnI GGCC 2 cut(s) 647, 739
Bsp143I GATC 2 cut(s) 354, 434
BspACI CCGC 7 cut(s) 60, 95, 239, 423, 428, 495, 598
BspANI GGCC 2 cut(s) 647, 739
BspCNI CTCAG 1 cut(s) 264
BspDI ATCGAT 1 cut(s) 485
BspFNI CGCG 1 cut(s) 600
BspLI GGNNCC 2 cut(s) 106, 557
BspMAI CTGCAG 1 cut(s) 390
BspMI ACCTGC 1 cut(s) 40
BspOI GCTAGC 1 cut(s) 60
BspPI GGATC 1 cut(s) 442
BspT107I GGYRCC 2 cut(s) 104, 555
BspTI CTTAAG 1 cut(s) 185
BsrI ACTGG 1 cut(s) 79
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 2 cut(s) 354, 434
Bst2UI CCWGG 1 cut(s) 768
Bst4CI ACNGT 3 cut(s) 304, 540, 654
BstAFI CTTAAG 1 cut(s) 185
BstBAI YACGTR 1 cut(s) 689
BstC8I GCNNGC 2 cut(s) 58, 280
BstDEI CTNAG 1 cut(s) 272
BstDSI CCRYGG 1 cut(s) 51
BstFNI CGCG 1 cut(s) 600
BstKTI GATC 2 cut(s) 357, 437
BstMBI GATC 2 cut(s) 354, 434
BstMWI GCNNNNNNNGC 4 cut(s) 125, 323, 428, 521
BstNI CCWGG 1 cut(s) 768
BstNSI RCATGY 1 cut(s) 195
BstSCI CCNGG 2 cut(s) 35, 766
BstSFI CTRYAG 1 cut(s) 386
BstUI CGCG 1 cut(s) 600
BstV1I GCAGC 4 cut(s) 223, 329, 412, 768
BstX2I RGATCY 1 cut(s) 434
BstXI CCANNNNNNTGG 1 cut(s) 774
BstYI RGATCY 1 cut(s) 434
Bsu15I ATCGAT 1 cut(s) 485
BsuI GTATCC 1 cut(s) 253
BsuRI GGCC 2 cut(s) 647, 739
BsuTUI ATCGAT 1 cut(s) 485
BtgI CCRYGG 1 cut(s) 51
BtsI GCAGTG 2 cut(s) 383, 405
BtsIMutI CAGTG 2 cut(s) 383, 405
BveI ACCTGC 1 cut(s) 40
Cac8I GCNNGC 2 cut(s) 58, 280
CaiI CAGNNNCTG 1 cut(s) 74
ClaI ATCGAT 1 cut(s) 485
Csp6I GTAC 2 cut(s) 562, 690
CviAII CATG 5 cut(s) 192, 361, 520, 552, 795
CviQI GTAC 2 cut(s) 562, 690
DdeI CTNAG 1 cut(s) 272
DpnI GATC 2 cut(s) 356, 436
DpnII GATC 2 cut(s) 354, 434
Eco147I AGGCCT 1 cut(s) 739
Eco57I CTGAAG 1 cut(s) 726
EcoRI GAATTC 1 cut(s) 64
EcoRII CCWGG 1 cut(s) 766
FaeI CATG 5 cut(s) 195, 364, 523, 555, 798
FalI AAGNNNNNCTT 2 cut(s) 168, 200
FaqI GGGAC 1 cut(s) 549
FatI CATG 5 cut(s) 191, 360, 519, 551, 794
FauI CCCGC 1 cut(s) 53
FbaI TGATCA 1 cut(s) 354
FblI GTMKAC 1 cut(s) 781
Fnu4HI GCNGC 6 cut(s) 237, 318, 423, 426, 429, 757
Fsp4HI GCNGC 6 cut(s) 237, 318, 423, 426, 429, 757
FspBI CTAG 5 cut(s) 57, 158, 288, 440, 732
GluI GCNGC 6 cut(s) 237, 318, 423, 426, 429, 757
HaeIII GGCC 2 cut(s) 647, 739
HapII CCGG 1 cut(s) 36
Hin1II CATG 5 cut(s) 195, 364, 523, 555, 798
HinfI GANTC 4 cut(s) 40, 122, 246, 487
HpaII CCGG 1 cut(s) 36
HphI GGTGA 1 cut(s) 21
Hpy166II GTNNAC 1 cut(s) 782
Hpy188I TCNGA 2 cut(s) 273, 544
Hpy188III TCNNGA 4 cut(s) 250, 288, 440, 451
Hpy8I GTNNAC 1 cut(s) 782
HpyAV CCTTC 3 cut(s) 317, 729, 750
HpyCH4III ACNGT 3 cut(s) 304, 540, 654
HpyCH4IV ACGT 5 cut(s) 312, 331, 369, 393, 688
HpyCH4V TGCA 6 cut(s) 215, 317, 388, 621, 686, 721
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 323, 428, 521
HpyF3I CTNAG 1 cut(s) 272
HpySE526I ACGT 5 cut(s) 312, 331, 369, 393, 688
Hsp92II CATG 5 cut(s) 195, 364, 523, 555, 798
Ksp22I TGATCA 1 cut(s) 354
Kzo9I GATC 2 cut(s) 354, 434
Lsp1109I GCAGC 4 cut(s) 223, 329, 412, 768
LweI GCATC 1 cut(s) 630
MaeI CTAG 5 cut(s) 57, 158, 288, 440, 732
MaeII ACGT 5 cut(s) 312, 331, 369, 393, 688
MaeIII GTNAC 1 cut(s) 572
MalI GATC 2 cut(s) 356, 436
MboI GATC 2 cut(s) 354, 434
MboII GAAGA 1 cut(s) 188
MfeI CAATTG 1 cut(s) 628
MflI RGATCY 1 cut(s) 434
MluCI AATT 3 cut(s) 44, 64, 628
MmeI TCCRAC 1 cut(s) 564
MnlI CCTC 4 cut(s) 212, 284, 367, 485
MroXI GAANNNNTTC 1 cut(s) 739
MseI TTAA 2 cut(s) 47, 186
MslI CAYNNNNRTG 1 cut(s) 799
MspA1I CMGCKG 2 cut(s) 95, 425
MspCI CTTAAG 1 cut(s) 185
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 2 cut(s) 37, 768
MunI CAATTG 1 cut(s) 628
MvaI CCWGG 1 cut(s) 768
MvnI CGCG 1 cut(s) 600
MwoI GCNNNNNNNGC 4 cut(s) 125, 323, 428, 521
NciI CCSGG 1 cut(s) 37
NdeII GATC 2 cut(s) 354, 434
NheI GCTAGC 1 cut(s) 56
NlaIII CATG 5 cut(s) 195, 364, 523, 555, 798
NlaIV GGNNCC 2 cut(s) 106, 557
NspI RCATGY 1 cut(s) 195
PaqCI CACCTGC 1 cut(s) 40
PceI AGGCCT 1 cut(s) 739
PciI ACATGT 1 cut(s) 191
PdmI GAANNNNTTC 1 cut(s) 739
PfeI GAWTC 4 cut(s) 40, 122, 246, 487
PflFI GACNNNGTC 1 cut(s) 446
PfoI TCCNGGA 1 cut(s) 766
PkrI GCNGC 6 cut(s) 238, 319, 424, 427, 430, 758
Ppu21I YACGTR 1 cut(s) 689
PscI ACATGT 1 cut(s) 191
Psp6I CCWGG 1 cut(s) 766
PspGI CCWGG 1 cut(s) 766
PspN4I GGNNCC 2 cut(s) 106, 557
PstI CTGCAG 1 cut(s) 390
PstNI CAGNNNCTG 1 cut(s) 74
PsuI RGATCY 1 cut(s) 434
PsyI GACNNNGTC 1 cut(s) 446
RsaI GTAC 2 cut(s) 563, 691
RsaNI GTAC 2 cut(s) 562, 690
RseI CAYNNNNRTG 1 cut(s) 799
SaqAI TTAA 2 cut(s) 47, 186
SatI GCNGC 6 cut(s) 237, 318, 423, 426, 429, 757
Sau3AI GATC 2 cut(s) 354, 434
ScrFI CCNGG 2 cut(s) 37, 768
SfaNI GCATC 1 cut(s) 630
SfcI CTRYAG 1 cut(s) 386
SmiMI CAYNNNNRTG 1 cut(s) 799
SmlI CTYRAG 1 cut(s) 185
SmoI CTYRAG 1 cut(s) 185
Sse9I AATT 3 cut(s) 44, 64, 628
SseBI AGGCCT 1 cut(s) 739
SsiI CCGC 7 cut(s) 60, 95, 239, 423, 428, 495, 598
SspMI CTAG 5 cut(s) 57, 158, 288, 440, 732
StuI AGGCCT 1 cut(s) 739
StyD4I CCNGG 2 cut(s) 35, 766
TaaI ACNGT 3 cut(s) 304, 540, 654
TaiI ACGT 5 cut(s) 315, 334, 372, 396, 691
TaqI TCGA 2 cut(s) 485, 490
TasI AATT 3 cut(s) 44, 64, 628
TauI GCSGC 2 cut(s) 425, 431
TfiI GAWTC 4 cut(s) 40, 122, 246, 487
Tru1I TTAA 2 cut(s) 47, 186
Tru9I TTAA 2 cut(s) 47, 186
TscAI CASTG 2 cut(s) 390, 412
TseI GCWGC 4 cut(s) 236, 317, 425, 756
TspDTI ATGAA 3 cut(s) 312, 326, 380
TspRI CASTG 2 cut(s) 390, 412
Tth111I GACNNNGTC 1 cut(s) 446
Vha464I CTTAAG 1 cut(s) 185
XapI RAATTY 1 cut(s) 64
XbaI TCTAGA 2 cut(s) 287, 439
XceI RCATGY 1 cut(s) 195
XmiI GTMKAC 1 cut(s) 781
XmnI GAANNNNTTC 1 cut(s) 739
XspI CTAG 5 cut(s) 57, 158, 288, 440, 732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.