Rw5G026160

Ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000157
Physical Location & Seq
Reverse (-)
37584879 .. 37587402
2524 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G026160.1

Sequence Viewer

Length: 1872 bp
ATGTCTATCCCACAAGCACAACATGTATCACCTTCCCAGATTCAATTAACCACAGCTAGCAGCGATTCAGTGACTGGGATAGACAACCAATCTGCAGACATCGCCATCCACATTTCAGCTGATCATTCGGCTCAACCTAACAGACCTTCGTCTTATTTACTAGAACATACAAACAGAGAAGAATCCCTTAAGACACGTGTTGCCCTCTATAAGTCTGCACTAAAAGGCGATTGGGAAGCTGCTGAACTAATCTTGAAAAAGGATAGGAGCAGACTTCTGCGAGCTAGCATATCTAGAGGATATGATACAGTCCTTCACGTTGCAGCAGGAACAAGCCATATTCACTTTGTGGAGAAACTGGTCAGCATGATGGAAGTAGAAGACTTAGCACTGCAGGACGTAAATGGAAACACAGCCTTGTGCATTGCTGCTTCGGCTGGATCTCTAGACATTGTCCAGATTTTGATACAAAAGAACAGGTCATTGCTAAAAATTAGAGGCGGTAAAGGTATGGCACCGGCCTATATGGCTGCTGTGTTAGGACAGTCCCAAATGGCATGTTACCTGTACCGGTACCGTGAAAGTAAAGACATGCTGGAGAAAACCGACCGAGAAAACTTGTTTTTTTCTTGCATCAACAATTGTCTTTACGATTTGGCCTTTCAGTTGCTAGATACTGATAAAGCATTAGCGAAGGCACGTACTAGTTCGCAGAAAAATAAAACGGCCTTGCATATCCTGGCTCGAAGACCTTCAGCATTTGGTGGCAGCCAAAGTCCACCAATGTGGACTAGACTCATCAACTCATTCCTCCCAGGTTTGAAGTTCGAATGCAAAAGAAAGTTGAGAGAAGCTAATGGAGCCTTTAAACTAGTCCACAGCCTCTGGGAAGAAATATTGAGAGATGAGCATGATAACGTGATGAGGCTGATCAAATATCCTTCAAATTTATTATTTGATGCAGCGGAATTAGGGAATTATGAGTTTCTGGCAATGCTTATGAGCTCTTATCCTGAGTTAGTATGGGAAACTAATGAAAAAAATCGAACCATAATCCATGTCGCAGTTCTGCATCGTCACGCAAGTATCTTCAACCTAGTGCATGAAATAGGCTCAATCAAAGATGTCATCGTGACATATGAAGATGATCAGGGTAACAACATTGTGCATATGGCTGCAAAATTAGCACCTCAAAATCAATTGAATCTCGAGTCAGGCGTGGCTCTTCAAATGCATCGAGAGTTGGTATGGTTTGAGGAAGTCAAAAAGATCGTCCAACCTCAATACATCGAGATGGAAAATAACGAAGGAAAAACACCTCAAGAATTATTTACTGAAGAGCATATGGTGTTAATGGGTGAAGGAGAAAAATGGATGAAGGACACTGCAACTTCATGCATGCTCGTTGCAACCATTATCGCAACCGTAGTGTTCTCAGCTGCATTTAGCATACCAGGTGGCACAGATGATCATACTGGAAAACCAAAACTTTTGAAACAGAAAGCCTTTCTAGACTTTACCATAGCTGACGAAGTAGCACTCTTTTCCTCTTCAACTGCAATGCTGATGTTCTTGTTCATCCTTACCTCGCGGTATGCAGAAAATGATTTCCGAAAGTCCTTACCCTTGAAGTTGATGGCAGGACTCACTAGCCTCTTCGTATCTATAGCATCGATGATGATGGCTTTCAGCACTGCCTTCTATCTATCTTGTCAATACGAATCAAAATGGGTTTCAAATTTTACATTTTTATTTGCATTTGTCCCGATTGCTTTGTTTGCACTTATGAAATTTCCTCTTGTGTCCGACATGTCCTCTTCTATATTTTGCTCAAGCCTACTATTTCAGCCGTGGAAACACATGCTCTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

623

Amino Acids

70.08

Weight (kDa)

6.56

Isoelectric Point (pI)

42.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 73 - 159 2.2e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 105 - 192 7.5e-10 Ankyrin repeats (3 copies)
PGG PF13962 456 - 569 1e-26 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54070 AT5G35830
fragaria_vesca FvH4_3g25070 FvH4_3g25080 FvH4_3g25081 FvH4_3g25081
malus_domestica MD03G1027500.v1.1 MD03G1146300.v1.1 MD03G1146800.v1.1 MD03G1146900.v1.1 MD03G1147100.v1.1 MD11G1165600.v1.1
prunus_persica Prupe.2G015800_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G016300_v2.0.a1 Prupe.2G016500_v2.0.a1 Prupe.6G131100_v2.0.a1 Prupe.6G131400_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1
pyrus_communis pycom03g10090 pycom03g10110 pycom11g13740 pycom11g13770
rosa_chinensis RchiOBHm_Chr1g0316371 RchiOBHm_Chr1g0316381 RchiOBHm_Chr1g0316401 RchiOBHm_Chr1g0316411 RchiOBHm_Chr1g0316571 RchiOBHm_Chr1g0316601 RchiOBHm_Chr5g0039341 RchiOBHm_Chr5g0041371 RchiOBHm_Chr5g0041401 RchiOBHm_Chr5g0041431 RchiOBHm_Chr5g0041471 RchiOBHm_Chr5g0045281 RchiOBHm_Chr5g0045291 RchiOBHm_Chr5g0045311 RchiOBHm_Chr5g0045371 RchiOBHm_Chr5g0045421 RchiOBHm_Chr5g0045431 RchiOBHm_Chr5g0045501 RchiOBHm_Chr5g0045551 RchiOBHm_Chr5g0045771 RchiOBHm_Chr5g0045811
rosa_laevigata RLG00000034057 RLG00000034058
rosa_multiflora Rmu_co8352751.1_g000001 Rmu_co8397575.1_g000001 Rmu_sc0000415.1_g000006 Rmu_sc0000493.1_g000057 Rmu_sc0000504.1_g000003 Rmu_sc0000594.1_g000019 Rmu_sc0000594.1_g000032 Rmu_sc0000594.1_g000066 Rmu_sc0001047.1_g000003 Rmu_sc0001047.1_g000011 Rmu_sc0001556.1_g000023 Rmu_sc0002584.1_g000003 Rmu_sc0002584.1_g000039 Rmu_sc0002584.1_g000070 Rmu_sc0002667.1_g000015 Rmu_sc0003677.1_g000003 Rmu_sc0003743.1_g000017 Rmu_sc0004520.1_g000004 Rmu_sc0004520.1_g000013 Rmu_sc0004520.1_g000027 Rmu_sc0005539.1_g000014 Rmu_sc0005539.1_g000032 Rmu_sc0006018.1_g000006 Rmu_sc0006737.1_g000020 Rmu_sc0007485.1_g000002 Rmu_sc0008729.1_g000002 Rmu_sc0008729.1_g000003 Rmu_sc0038822.1_g000001 Rmu_ssc0000398.1_g000025
rosa_roxburghii Rroxscaffold_1G00035090 Rroxscaffold_1G00035630 Rroxscaffold_1G00035640 Rroxscaffold_1G00035660 Rroxscaffold_1G00035680 Rroxscaffold_1G00035730 Rroxscaffold_1G00035750 Rroxscaffold_1G00035770 Rroxscaffold_1G00039150 Rroxscaffold_1G00039190 Rroxscaffold_1G00039220 Rroxscaffold_1G00039260 Rroxscaffold_4G00328910 Rroxscaffold_4G00328950 Rroxscaffold_4G00328960 Rroxscaffold_4G00330890
rosa_rugosa Rorug01G0009800 Rorug01G0010000.1 Rorug01G0010200 Rorug01G0012100 Rorug01G0023500 Rorug01G0023600 Rorug01G0023700 Rorug01G0024700 Rorug05G0192000 Rorug05G0192200 Rorug05G0222400 Rorug05G0222500 Rorug05G0222600.1 Rorug05G0222700.1 Rorug05G0222800.1 Rorug05G0222900.1 Rorug05G0223000.1 Rorug05G0223100.1 Rorug05G0223200 Rorug05G0223600 Rorug05G0226800 Rorug05G0226900
rosa_samantha Rh1CG019600 Rh1DG015000 Rh1DG015300 Rh1DG016900 Rh5CG315200 Rh5CG338700 Rh5DG277100 Rh5DG291500 Rh5DG292100 Rh5DG292200 Rh5DG292400 Rh5DG292600 Rh5DG322100 Rh5DG322200 Rh5DG323100 Rh5DG323300 Rh5DG326700 Rh5DG326900 Rh5DG327000
rosa_wichuraiana Rw0G021580 Rw1G001320 Rw1G001420 Rw1G002820 Rw1G002840 Rw1G002870 Rw5G024800 Rw5G026160 Rw5G026170 Rw5G026180 Rw5G026210 Rw5G026230 Rw5G028170 Rw5G028190 Rw5G028210 Rw5G028230 Rw5G028240 Rw5G028260 Rw5G028280 Rw5G028370 Rw5G028380 Rw5G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 573
AccB1I GGYRCC 2 cut(s) 514, 573
AccII CGCG 1 cut(s) 1591
AciI CCGC 3 cut(s) 501, 965, 1591
AclWI GGATC 1 cut(s) 448
AcsI RAATTY 3 cut(s) 946, 1738, 1790
AcuI CTGAAG 2 cut(s) 738, 1356
AcvI CACGTG 1 cut(s) 197
AdeI CACNNNGTG 1 cut(s) 349
AfaI GTAC 3 cut(s) 569, 575, 703
AflII CTTAAG 1 cut(s) 188
AflIII ACRYGT 4 cut(s) 22, 194, 196, 1809
AgeI ACCGGT 1 cut(s) 570
AhlI ACTAGT 2 cut(s) 704, 871
AjnI CCWGG 3 cut(s) 738, 814, 1453
AleI CACNNNNGTG 1 cut(s) 784
AluBI AGCT 8 cut(s) 56, 119, 239, 284, 854, 1005, 1439, 1526
AluI AGCT 8 cut(s) 56, 119, 239, 284, 854, 1005, 1439, 1526
Alw21I GWGCWC 1 cut(s) 1007
AlwI GGATC 1 cut(s) 448
AlwNI CAGNNNCTG 2 cut(s) 74, 885
Ama87I CYCGRG 1 cut(s) 1208
AoxI GGCC 3 cut(s) 519, 657, 726
ApeKI GCWGC 9 cut(s) 60, 239, 323, 428, 530, 768, 962, 1175, 1439
ApoI RAATTY 3 cut(s) 946, 1738, 1790
ArsI GACNNNNNNTTYG 2 cut(s) 1257, 1289
AsiGI ACCGGT 1 cut(s) 570
Asp700I GAANNNNTTC 1 cut(s) 751
Asp718I GGTACC 1 cut(s) 573
AsuHPI GGTGA 2 cut(s) 21, 1370
AsuII TTCGAA 1 cut(s) 828
AsuNHI GCTAGC 2 cut(s) 56, 284
AvaI CYCGRG 1 cut(s) 1208
BaeI ACNNNNGTAYC 2 cut(s) 1069, 1102
BanI GGYRCC 2 cut(s) 514, 573
BanII GRGCYC 1 cut(s) 1007
BbrPI CACGTG 1 cut(s) 197
BbsI GAAGAC 2 cut(s) 387, 754
Bbv12I GWGCWC 1 cut(s) 1007
BbvI GCAGC 9 cut(s) 72, 226, 335, 415, 517, 780, 974, 1162, 1426
BccI CCATC 5 cut(s) 113, 364, 1288, 1630, 1675
BceAI ACGGC 2 cut(s) 741, 1834
BciT130I CCWGG 3 cut(s) 740, 816, 1455
BclI TGATCA 4 cut(s) 121, 930, 1147, 1468
BcuI ACTAGT 2 cut(s) 704, 871
BfmI CTRYAG 3 cut(s) 93, 392, 1665
BfrI CTTAAG 1 cut(s) 188
BglI GCCNNNNNGGC 1 cut(s) 527
BisI GCNGC 9 cut(s) 61, 240, 324, 429, 531, 769, 963, 1176, 1440
BlsI GCNGC 9 cut(s) 62, 241, 325, 430, 532, 770, 964, 1177, 1441
Bme1390I CCNGG 3 cut(s) 740, 816, 1455
BmeT110I CYCGRG 1 cut(s) 1208
BmiI GGNNCC 3 cut(s) 516, 575, 862
BmrFI CCNGG 3 cut(s) 740, 816, 1455
BmrI ACTGGG 1 cut(s) 84
BmsI GCATC 5 cut(s) 642, 949, 1081, 1243, 1679
BmtI GCTAGC 2 cut(s) 60, 288
BmuI ACTGGG 1 cut(s) 84
BoxI GACNNNNGTC 1 cut(s) 148
BpiI GAAGAC 2 cut(s) 387, 754
BpmI CTGGAG 1 cut(s) 617
Bpu14I TTCGAA 1 cut(s) 828
BpuEI CTTGAG 2 cut(s) 1305, 1816
Bsa29I ATCGAT 1 cut(s) 1673
BsaAI YACGTR 2 cut(s) 197, 701
BsaJI CCNNGG 2 cut(s) 814, 1850
BsaWI WCCGGW 1 cut(s) 570
Bse118I RCCGGY 2 cut(s) 517, 570
Bse1I ACTGG 3 cut(s) 79, 363, 1480
Bse3DI GCAATG 4 cut(s) 423, 482, 999, 1566
BseBI CCWGG 3 cut(s) 740, 816, 1455
BseCI ATCGAT 1 cut(s) 1673
BseDI CCNNGG 2 cut(s) 814, 1850
BseGI GGATG 3 cut(s) 105, 1380, 1578
BseMI GCAATG 4 cut(s) 423, 482, 999, 1566
BseMII CTCAG 2 cut(s) 1005, 1449
BseNI ACTGG 3 cut(s) 79, 363, 1480
BseXI GCAGC 9 cut(s) 72, 226, 335, 415, 517, 780, 974, 1162, 1426
BsgI GTGCAG 1 cut(s) 201
Bsh1236I CGCG 1 cut(s) 1591
Bsh1285I CGRYCG 1 cut(s) 610
BshFI GGCC 3 cut(s) 521, 659, 728
BshNI GGYRCC 2 cut(s) 514, 573
BshTI ACCGGT 1 cut(s) 570
BshVI ATCGAT 1 cut(s) 1673
BsiEI CGRYCG 1 cut(s) 610
BsiHKAI GWGCWC 1 cut(s) 1007
BsiHKCI CYCGRG 1 cut(s) 1208
BsiSI CCGG 2 cut(s) 518, 571
BslFI GGGAC 2 cut(s) 532, 1748
BsmFI GGGAC 2 cut(s) 532, 1748
BsmI GAATGC 1 cut(s) 836
BsnI GGCC 3 cut(s) 521, 659, 728
BsoBI CYCGRG 1 cut(s) 1208
Bsp119I TTCGAA 1 cut(s) 828
Bsp1286I GDGCHC 1 cut(s) 1007
Bsp143I GATC 6 cut(s) 121, 440, 930, 1147, 1269, 1468
BspACI CCGC 3 cut(s) 501, 965, 1591
BspANI GGCC 3 cut(s) 521, 659, 728
BspCNI CTCAG 2 cut(s) 1006, 1448
BspDI ATCGAT 1 cut(s) 1673
BspFNI CGCG 1 cut(s) 1591
BspLI GGNNCC 3 cut(s) 516, 575, 862
BspMAI CTGCAG 2 cut(s) 97, 396
BspOI GCTAGC 2 cut(s) 60, 288
BspPI GGATC 1 cut(s) 448
BspQI GCTCTTC 2 cut(s) 1230, 1332
BspT104I TTCGAA 1 cut(s) 828
BspT107I GGYRCC 2 cut(s) 514, 573
BspTI CTTAAG 1 cut(s) 188
BsrDI GCAATG 4 cut(s) 423, 482, 999, 1566
BsrFI RCCGGY 2 cut(s) 517, 570
BsrI ACTGG 3 cut(s) 79, 363, 1480
BssAI RCCGGY 2 cut(s) 517, 570
BssECI CCNNGG 2 cut(s) 814, 1850
BssMI GATC 6 cut(s) 121, 440, 930, 1147, 1269, 1468
Bst2UI CCWGG 3 cut(s) 740, 816, 1455
Bst4CI ACNGT 4 cut(s) 310, 546, 578, 1426
Bst6I CTCTTC 5 cut(s) 1230, 1332, 1555, 1661, 1822
BstAFI CTTAAG 1 cut(s) 188
BstBAI YACGTR 2 cut(s) 197, 701
BstBI TTCGAA 1 cut(s) 828
BstC8I GCNNGC 4 cut(s) 58, 282, 286, 1400
BstDEI CTNAG 3 cut(s) 385, 1014, 1435
BstDSI CCRYGG 1 cut(s) 1850
BstF5I GGATG 3 cut(s) 105, 1380, 1578
BstFNI CGCG 1 cut(s) 1591
BstKTI GATC 6 cut(s) 124, 443, 933, 1150, 1272, 1471
BstMBI GATC 6 cut(s) 121, 440, 930, 1147, 1269, 1468
BstMCI CGRYCG 1 cut(s) 610
BstMWI GCNNNNNNNGC 6 cut(s) 101, 434, 527, 860, 1184, 1778
BstNI CCWGG 3 cut(s) 740, 816, 1455
BstNSI RCATGY 6 cut(s) 26, 561, 595, 1402, 1813, 1864
BstPAI GACNNNNGTC 1 cut(s) 148
BstSCI CCNGG 3 cut(s) 738, 814, 1453
BstSFI CTRYAG 3 cut(s) 93, 392, 1665
BstUI CGCG 1 cut(s) 1591
BstV1I GCAGC 9 cut(s) 72, 226, 335, 415, 517, 780, 974, 1162, 1426
BstV2I GAAGAC 2 cut(s) 387, 754
BstX2I RGATCY 1 cut(s) 440
BstXI CCANNNNNNTGG 1 cut(s) 786
BstYI RGATCY 1 cut(s) 440
Bsu15I ATCGAT 1 cut(s) 1673
BsuRI GGCC 3 cut(s) 521, 659, 728
BsuTUI ATCGAT 1 cut(s) 1673
BtgI CCRYGG 1 cut(s) 1850
BtgZI GCGATG 1 cut(s) 85
BtsCI GGATG 3 cut(s) 105, 1380, 1578
BtsI GCAGTG 3 cut(s) 389, 1383, 1692
BtsIMutI CAGTG 4 cut(s) 75, 389, 1383, 1692
Cac8I GCNNGC 4 cut(s) 58, 282, 286, 1400
CaiI CAGNNNCTG 2 cut(s) 74, 885
Cfr10I RCCGGY 2 cut(s) 517, 570
ClaI ATCGAT 1 cut(s) 1673
CsiI ACCWGGT 1 cut(s) 1453
Csp6I GTAC 3 cut(s) 568, 574, 702
CspAI ACCGGT 1 cut(s) 570
CviQI GTAC 3 cut(s) 568, 574, 702
DdeI CTNAG 3 cut(s) 385, 1014, 1435
DpnI GATC 6 cut(s) 123, 442, 932, 1149, 1271, 1470
DpnII GATC 6 cut(s) 121, 440, 930, 1147, 1269, 1468
DraI TTTAAA 1 cut(s) 868
DraIII CACNNNGTG 1 cut(s) 349
Eam1104I CTCTTC 5 cut(s) 1230, 1332, 1555, 1661, 1822
EarI CTCTTC 5 cut(s) 1230, 1332, 1555, 1661, 1822
Ecl136II GAGCTC 1 cut(s) 1005
Eco24I GRGCYC 1 cut(s) 1007
Eco53kI GAGCTC 1 cut(s) 1005
Eco57I CTGAAG 2 cut(s) 738, 1356
Eco72I CACGTG 1 cut(s) 197
Eco88I CYCGRG 1 cut(s) 1208
EcoICRI GAGCTC 1 cut(s) 1005
EcoRII CCWGG 3 cut(s) 738, 814, 1453
EcoT22I ATGCAT 2 cut(s) 1236, 1400
EcoT38I GRGCYC 1 cut(s) 1007
FalI AAGNNNNNCTT 2 cut(s) 171, 203
FaqI GGGAC 2 cut(s) 532, 1748
FauNDI CATATG 3 cut(s) 1138, 1170, 1344
FbaI TGATCA 4 cut(s) 121, 930, 1147, 1468
Fnu4HI GCNGC 9 cut(s) 61, 240, 324, 429, 531, 769, 963, 1176, 1440
FokI GGATG 3 cut(s) 92, 1387, 1565
FriOI GRGCYC 1 cut(s) 1007
Fsp4HI GCNGC 9 cut(s) 61, 240, 324, 429, 531, 769, 963, 1176, 1440
GluI GCNGC 9 cut(s) 61, 240, 324, 429, 531, 769, 963, 1176, 1440
GsuI CTGGAG 1 cut(s) 617
HaeIII GGCC 3 cut(s) 521, 659, 728
HapII CCGG 2 cut(s) 518, 571
HinfI GANTC 8 cut(s) 40, 65, 182, 795, 1204, 1211, 1644, 1721
HpaII CCGG 2 cut(s) 518, 571
HphI GGTGA 2 cut(s) 21, 1370
Hpy166II GTNNAC 3 cut(s) 779, 789, 877
Hpy188I TCNGA 2 cut(s) 1613, 1807
Hpy8I GTNNAC 3 cut(s) 779, 789, 877
HpyCH4III ACNGT 4 cut(s) 310, 546, 578, 1426
HpyCH4IV ACGT 5 cut(s) 196, 318, 399, 700, 918
HpyF10VI GCNNNNNNNGC 6 cut(s) 101, 434, 527, 860, 1184, 1778
HpyF3I CTNAG 3 cut(s) 385, 1014, 1435
HpySE526I ACGT 5 cut(s) 196, 318, 399, 700, 918
KpnI GGTACC 1 cut(s) 577
Ksp22I TGATCA 4 cut(s) 121, 930, 1147, 1468
Kzo9I GATC 6 cut(s) 121, 440, 930, 1147, 1269, 1468
LguI GCTCTTC 2 cut(s) 1230, 1332
LmnI GCTCC 2 cut(s) 267, 860
Lsp1109I GCAGC 9 cut(s) 72, 226, 335, 415, 517, 780, 974, 1162, 1426
LweI GCATC 5 cut(s) 642, 949, 1081, 1243, 1679
MabI ACCWGGT 1 cut(s) 1453
MaeII ACGT 5 cut(s) 196, 318, 399, 700, 918
MaeIII GTNAC 5 cut(s) 70, 560, 1076, 1132, 1154
MalI GATC 6 cut(s) 123, 442, 932, 1149, 1271, 1470
MboI GATC 6 cut(s) 121, 440, 930, 1147, 1269, 1468
MfeI CAATTG 2 cut(s) 640, 1199
MflI RGATCY 1 cut(s) 440
MhlI GDGCHC 1 cut(s) 1007
MlyI GAGTC 3 cut(s) 789, 1220, 1638
MmeI TCCRAC 2 cut(s) 1300, 1830
Mph1103I ATGCAT 2 cut(s) 1236, 1400
MroXI GAANNNNTTC 1 cut(s) 751
MseI TTAA 4 cut(s) 47, 189, 867, 1352
MslI CAYNNNNRTG 2 cut(s) 784, 1292
MspA1I CMGCKG 3 cut(s) 119, 965, 1439
MspCI CTTAAG 1 cut(s) 188
MspI CCGG 2 cut(s) 518, 571
MspR9I CCNGG 3 cut(s) 740, 816, 1455
MunI CAATTG 2 cut(s) 640, 1199
Mva1269I GAATGC 1 cut(s) 836
MvaI CCWGG 3 cut(s) 740, 816, 1455
MvnI CGCG 1 cut(s) 1591
MwoI GCNNNNNNNGC 6 cut(s) 101, 434, 527, 860, 1184, 1778
NdeI CATATG 3 cut(s) 1138, 1170, 1344
NdeII GATC 6 cut(s) 121, 440, 930, 1147, 1269, 1468
NheI GCTAGC 2 cut(s) 56, 284
NlaIV GGNNCC 3 cut(s) 516, 575, 862
NmuCI GTSAC 3 cut(s) 70, 1076, 1132
NsiI ATGCAT 2 cut(s) 1236, 1400
NspI RCATGY 6 cut(s) 26, 561, 595, 1402, 1813, 1864
NspV TTCGAA 1 cut(s) 828
OliI CACNNNNGTG 1 cut(s) 784
PaeI GCATGC 1 cut(s) 1402
PaeR7I CTCGAG 1 cut(s) 1208
PciI ACATGT 2 cut(s) 22, 1809
PciSI GCTCTTC 2 cut(s) 1230, 1332
PcsI WCGNNNNNNNCGW 1 cut(s) 1215
PctI GAATGC 1 cut(s) 836
PdmI GAANNNNTTC 1 cut(s) 751
PfeI GAWTC 5 cut(s) 40, 65, 182, 1204, 1721
PflFI GACNNNGTC 1 cut(s) 452
PinAI ACCGGT 1 cut(s) 570
PkrI GCNGC 9 cut(s) 62, 241, 325, 430, 532, 770, 964, 1177, 1441
PleI GAGTC 3 cut(s) 789, 1219, 1638
PmaCI CACGTG 1 cut(s) 197
PmlI CACGTG 1 cut(s) 197
PpsI GAGTC 3 cut(s) 789, 1219, 1638
Ppu21I YACGTR 2 cut(s) 197, 701
PscI ACATGT 2 cut(s) 22, 1809
PshAI GACNNNNGTC 1 cut(s) 148
Psp124BI GAGCTC 1 cut(s) 1007
Psp6I CCWGG 3 cut(s) 738, 814, 1453
PspCI CACGTG 1 cut(s) 197
PspGI CCWGG 3 cut(s) 738, 814, 1453
PspN4I GGNNCC 3 cut(s) 516, 575, 862
PstI CTGCAG 2 cut(s) 97, 396
PstNI CAGNNNCTG 2 cut(s) 74, 885
PsuI RGATCY 1 cut(s) 440
PsyI GACNNNGTC 1 cut(s) 452
PvuII CAGCTG 2 cut(s) 119, 1439
RsaI GTAC 3 cut(s) 569, 575, 703
RsaNI GTAC 3 cut(s) 568, 574, 702
RseI CAYNNNNRTG 2 cut(s) 784, 1292
SacI GAGCTC 1 cut(s) 1007
SapI GCTCTTC 2 cut(s) 1230, 1332
SaqAI TTAA 4 cut(s) 47, 189, 867, 1352
SatI GCNGC 9 cut(s) 61, 240, 324, 429, 531, 769, 963, 1176, 1440
Sau3AI GATC 6 cut(s) 121, 440, 930, 1147, 1269, 1468
SchI GAGTC 3 cut(s) 789, 1220, 1638
ScrFI CCNGG 3 cut(s) 740, 816, 1455
SduI GDGCHC 1 cut(s) 1007
SexAI ACCWGGT 1 cut(s) 1453
SfaNI GCATC 5 cut(s) 642, 949, 1081, 1243, 1679
SfcI CTRYAG 3 cut(s) 93, 392, 1665
Sfr274I CTCGAG 1 cut(s) 1208
SfuI TTCGAA 1 cut(s) 828
SlaI CTCGAG 1 cut(s) 1208
SmiMI CAYNNNNRTG 2 cut(s) 784, 1292
SmlI CTYRAG 4 cut(s) 188, 1208, 1320, 1831
SmoI CTYRAG 4 cut(s) 188, 1208, 1320, 1831
SpeI ACTAGT 2 cut(s) 704, 871
SphI GCATGC 1 cut(s) 1402
SsiI CCGC 3 cut(s) 501, 965, 1591
SspI AATATT 1 cut(s) 897
SstI GAGCTC 1 cut(s) 1007
StyD4I CCNGG 3 cut(s) 738, 814, 1453
TaaI ACNGT 4 cut(s) 310, 546, 578, 1426
TaiI ACGT 5 cut(s) 199, 321, 402, 703, 921
TaqI TCGA 7 cut(s) 745, 828, 1045, 1209, 1237, 1290, 1673
TaqII GACCGA 1 cut(s) 624
TfiI GAWTC 5 cut(s) 40, 65, 182, 1204, 1721
Tru1I TTAA 4 cut(s) 47, 189, 867, 1352
Tru9I TTAA 4 cut(s) 47, 189, 867, 1352
TscAI CASTG 4 cut(s) 75, 396, 1390, 1699
TseFI GTSAC 3 cut(s) 70, 1076, 1132
TseI GCWGC 9 cut(s) 60, 239, 323, 428, 530, 768, 962, 1175, 1439
Tsp45I GTSAC 3 cut(s) 70, 1076, 1132
TspDTI ATGAA 7 cut(s) 1050, 1119, 1155, 1383, 1391, 1567, 1802
TspRI CASTG 4 cut(s) 75, 396, 1390, 1699
Tth111I GACNNNGTC 1 cut(s) 452
Vha464I CTTAAG 1 cut(s) 188
XapI RAATTY 3 cut(s) 946, 1738, 1790
XbaI TCTAGA 3 cut(s) 293, 445, 1510
XceI RCATGY 6 cut(s) 26, 561, 595, 1402, 1813, 1864
XhoI CTCGAG 1 cut(s) 1208
XmnI GAANNNNTTC 1 cut(s) 751
Zsp2I ATGCAT 2 cut(s) 1236, 1400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.