Rw5G026230

Ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000157
Physical Location & Seq
Reverse (-)
37919611 .. 37930462
10852 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G026230.1

Sequence Viewer

Length: 1848 bp
ATGTCTATCCCAAAAACACAACATGTATCACCTGCAGCTACCGGCGATTCAGCAACTGGAATAGGCAACCAATCAGCAGACATCACCATCGACATGTCAGCTGATTCGGCTCAACCTAACAGACCTTTGTCTTATTTACTAGTACATACAATGAGAGAAGAATACCTTAAGACATGTGTTTCCCTCTATAAGCATGCACTAAAAGGGGATTGGGAAGCGGCGGAACGAATCTTGAGAAAGGATACTATACTTCTGAGAGCAAGCATATCTAGAGGATATGAAACAGTCCTTCACGTTGCAGCCGGAACAAGCCATATTCACTTTGTGGAGAAACTGGTCAGCATGATGGAAGTAGAAGACTTGGCACTGCAGGACATAAATGGAAACACTGCCCTGTGCATTGCTGCTGCGGCTGGATCTCTAGACATTGTCAAGATTTTGATACAAAAGAACAAGTCTTTGCTAACAATTAGAGGCGGTAAAAATATGGCACCAGCTTATATGGCTGCTGTGTTAGGACAGTCCGAAATGGCATGTTACCTGTTGGAGAAAACCGAAAGTAAAATGATGTTGGAGAGAACCGACCGAGAAAATTTGTTCTTTTCTTGTATCAACAATTGTTTGTACGATTTGGCCTTTCAGTTGCTAGATGCTGATAAATCATTAGCGAAGGCACGTACTAGTTCACAGAAAAATAAAACGGCCTTGCATATCCTGGCTCGAAGACCTTCAGCATTTGGCGGCAGCCAAAGTCCACCAATGTGGACTAGACTCATTAACTCATTCCTCCCAGGTTTGAAGTTCGAATGCAAAAAAAAGTTGAGAGAAGCTAATGGAGCCATTCATCTAGTCCACCACCTCTGGGAAGAAATCTTGAGAGATGAGCATGATGACGTGATGAGGCTTATCAAATATCCTTCAAATTTATTATTTGATGCAGCGGAATTAGGGAATTATGAGTTTCTGGCAATGCTTATGAGCTCTTACCCCGAGTTAGTATGGGAAACTGATGAAAAAAATCGGACTATAATCCATGTCGCAGTTCTGCATCGTCACGCAAGTATCTTCAATCTAGTGCATGAAATAGGCTCAATCAAAGATGTCATCGTGACATATGAAGATGATCAGGGTAACAACATTGTGCATATGGCTGCAAAATTAGCACCTCAAAATCAACTGAATCTCGTGTCAGGCGTAGCATTTCAAATGCATCGAGAGTTGGTATGGTTTGAGGAAGTCAAAAAGATTGTCCAACCTCAATACATCGAGATGGAAAATGATGAAGGAAAAACACCTCAAGAATTATTTACTGAAGAGCATACAGTGTTAATGCGTGAAGGAGAAAAATGGATGAAGGACACTGCAACTTCATGCATGCTTGTTGCGACTATTATCGCAACCGTTGTGTTCTCAGCTGCATTTAGCATACCGGGTGGCACAGATGATCATACTGGAAAACCAAACCTTTTGAAACAGAAAACCTTTCTAGACTTTTCCATAGCTGACGAATTAGCACTCTTTTCCTCTTCAACTGCAATGTTGATGTTTTTGTTCATCCTTACCTCGCGGTATGCAGAAAATGATTTCCTCGTGTCCTTACCCTTGAAGTTGATGGTAGGACTCACTTGCCTCTTCATATCTATAGCATCGATGATGATGGCTTTCAGCACCGCCTTCTATCTATCTTGTCAATACGAATCAAAATGGGTTTCAAATTTTACATTTTTATTTGCGTTTGTCCCAATTGCTTTGTTTGCGTTTATGAAATTCCCTCTTGTGTCCGACATGTCCTCTTCTATATTTTGCTCAAGTCTACTATTTCAGCCGTGGAAACACATGCTCTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

615

Amino Acids

69.17

Weight (kDa)

6.0

Isoelectric Point (pI)

41.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 69 - 151 4e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 97 - 185 9.1e-11 Ankyrin repeats (3 copies)
PGG PF13962 448 - 561 5.7e-26 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54070 AT5G35830
fragaria_vesca FvH4_3g25070 FvH4_3g25080 FvH4_3g25081 FvH4_3g25081
malus_domestica MD03G1027500.v1.1 MD03G1146300.v1.1 MD03G1146800.v1.1 MD03G1146900.v1.1 MD03G1147100.v1.1 MD11G1165600.v1.1
prunus_persica Prupe.2G015800_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G016300_v2.0.a1 Prupe.2G016500_v2.0.a1 Prupe.6G131100_v2.0.a1 Prupe.6G131400_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1
pyrus_communis pycom03g10090 pycom03g10110 pycom11g13740 pycom11g13770
rosa_chinensis RchiOBHm_Chr1g0316371 RchiOBHm_Chr1g0316381 RchiOBHm_Chr1g0316401 RchiOBHm_Chr1g0316411 RchiOBHm_Chr1g0316571 RchiOBHm_Chr1g0316601 RchiOBHm_Chr5g0039341 RchiOBHm_Chr5g0041371 RchiOBHm_Chr5g0041401 RchiOBHm_Chr5g0041431 RchiOBHm_Chr5g0041471 RchiOBHm_Chr5g0045281 RchiOBHm_Chr5g0045291 RchiOBHm_Chr5g0045311 RchiOBHm_Chr5g0045371 RchiOBHm_Chr5g0045421 RchiOBHm_Chr5g0045431 RchiOBHm_Chr5g0045501 RchiOBHm_Chr5g0045551 RchiOBHm_Chr5g0045771 RchiOBHm_Chr5g0045811
rosa_laevigata RLG00000034057 RLG00000034058
rosa_multiflora Rmu_co8352751.1_g000001 Rmu_co8397575.1_g000001 Rmu_sc0000415.1_g000006 Rmu_sc0000493.1_g000057 Rmu_sc0000504.1_g000003 Rmu_sc0000594.1_g000019 Rmu_sc0000594.1_g000032 Rmu_sc0000594.1_g000066 Rmu_sc0001047.1_g000003 Rmu_sc0001047.1_g000011 Rmu_sc0001556.1_g000023 Rmu_sc0002584.1_g000003 Rmu_sc0002584.1_g000039 Rmu_sc0002584.1_g000070 Rmu_sc0002667.1_g000015 Rmu_sc0003677.1_g000003 Rmu_sc0003743.1_g000017 Rmu_sc0004520.1_g000004 Rmu_sc0004520.1_g000013 Rmu_sc0004520.1_g000027 Rmu_sc0005539.1_g000014 Rmu_sc0005539.1_g000032 Rmu_sc0006018.1_g000006 Rmu_sc0006737.1_g000020 Rmu_sc0007485.1_g000002 Rmu_sc0008729.1_g000002 Rmu_sc0008729.1_g000003 Rmu_sc0038822.1_g000001 Rmu_ssc0000398.1_g000025
rosa_roxburghii Rroxscaffold_1G00035090 Rroxscaffold_1G00035630 Rroxscaffold_1G00035640 Rroxscaffold_1G00035660 Rroxscaffold_1G00035680 Rroxscaffold_1G00035730 Rroxscaffold_1G00035750 Rroxscaffold_1G00035770 Rroxscaffold_1G00039150 Rroxscaffold_1G00039190 Rroxscaffold_1G00039220 Rroxscaffold_1G00039260 Rroxscaffold_4G00328910 Rroxscaffold_4G00328950 Rroxscaffold_4G00328960 Rroxscaffold_4G00330890
rosa_rugosa Rorug01G0009800 Rorug01G0010000.1 Rorug01G0010200 Rorug01G0012100 Rorug01G0023500 Rorug01G0023600 Rorug01G0023700 Rorug01G0024700 Rorug05G0192000 Rorug05G0192200 Rorug05G0222400 Rorug05G0222500 Rorug05G0222600.1 Rorug05G0222700.1 Rorug05G0222800.1 Rorug05G0222900.1 Rorug05G0223000.1 Rorug05G0223100.1 Rorug05G0223200 Rorug05G0223600 Rorug05G0226800 Rorug05G0226900
rosa_samantha Rh1CG019600 Rh1DG015000 Rh1DG015300 Rh1DG016900 Rh5CG315200 Rh5CG338700 Rh5DG277100 Rh5DG291500 Rh5DG292100 Rh5DG292200 Rh5DG292400 Rh5DG292600 Rh5DG322100 Rh5DG322200 Rh5DG323100 Rh5DG323300 Rh5DG326700 Rh5DG326900 Rh5DG327000
rosa_wichuraiana Rw0G021580 Rw1G001320 Rw1G001420 Rw1G002820 Rw1G002840 Rw1G002870 Rw5G024800 Rw5G026160 Rw5G026170 Rw5G026180 Rw5G026210 Rw5G026230 Rw5G028170 Rw5G028190 Rw5G028210 Rw5G028230 Rw5G028240 Rw5G028260 Rw5G028280 Rw5G028370 Rw5G028380 Rw5G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 40
Acc36I ACCTGC 1 cut(s) 40
AccB1I GGYRCC 1 cut(s) 490
AccI GTMKAC 1 cut(s) 1813
AccII CGCG 1 cut(s) 1567
AciI CCGC 8 cut(s) 218, 221, 410, 477, 741, 941, 1567, 1671
AclWI GGATC 1 cut(s) 424
AcsI RAATTY 4 cut(s) 592, 922, 1714, 1766
AcuI CTGAAG 2 cut(s) 714, 1332
AdeI CACNNNGTG 1 cut(s) 325
AfaI GTAC 3 cut(s) 144, 626, 679
AfiI CCNNNNNNNGG 1 cut(s) 862
AflII CTTAAG 1 cut(s) 167
AflIII ACRYGT 4 cut(s) 22, 93, 173, 1785
AgsI TTSAA 8 cut(s) 799, 921, 1069, 1205, 1471, 1530, 1606, 1713
AhlI ACTAGT 2 cut(s) 139, 680
AjiI CACGTC 1 cut(s) 895
AjnI CCWGG 2 cut(s) 714, 790
AleI CACNNNNGTG 1 cut(s) 760
AluBI AGCT 7 cut(s) 38, 101, 497, 830, 981, 1415, 1502
AluI AGCT 7 cut(s) 38, 101, 497, 830, 981, 1415, 1502
Alw21I GWGCWC 1 cut(s) 983
AlwI GGATC 1 cut(s) 424
AlwNI CAGNNNCTG 1 cut(s) 56
Ama87I CYCGRG 1 cut(s) 989
AoxI GGCC 2 cut(s) 633, 702
ApeKI GCWGC 9 cut(s) 35, 299, 404, 407, 506, 744, 938, 1151, 1415
ApoI RAATTY 4 cut(s) 592, 922, 1714, 1766
ArsI GACNNNNNNTTYG 2 cut(s) 1233, 1265
Asp700I GAANNNNTTC 1 cut(s) 727
AsuC2I CCSGG 1 cut(s) 1431
AsuHPI GGTGA 2 cut(s) 21, 76
AsuII TTCGAA 1 cut(s) 804
AvaI CYCGRG 1 cut(s) 989
BaeI ACNNNNGTAYC 2 cut(s) 1045, 1078
BanI GGYRCC 1 cut(s) 490
BanII GRGCYC 1 cut(s) 983
BauI CACGAG 2 cut(s) 1184, 1589
BbsI GAAGAC 2 cut(s) 363, 730
Bbv12I GWGCWC 1 cut(s) 983
BbvI GCAGC 9 cut(s) 47, 311, 391, 394, 493, 756, 950, 1138, 1402
BccI CCATC 5 cut(s) 95, 340, 1264, 1606, 1651
BceAI ACGGC 2 cut(s) 717, 1810
BciT130I CCWGG 2 cut(s) 716, 792
BciVI GTATCC 1 cut(s) 235
BclI TGATCA 2 cut(s) 1123, 1444
BcnI CCSGG 1 cut(s) 1431
BcuI ACTAGT 2 cut(s) 139, 680
BfmI CTRYAG 3 cut(s) 33, 368, 1641
BfrI CTTAAG 1 cut(s) 167
BfuAI ACCTGC 1 cut(s) 40
BfuI GTATCC 1 cut(s) 235
Bme1390I CCNGG 3 cut(s) 716, 792, 1431
BmeT110I CYCGRG 1 cut(s) 989
BmgBI CACGTC 1 cut(s) 895
BmiI GGNNCC 2 cut(s) 492, 838
BmrFI CCNGG 3 cut(s) 716, 792, 1431
BmsI GCATC 5 cut(s) 640, 925, 1057, 1219, 1655
BoxI GACNNNNGTC 1 cut(s) 127
BpiI GAAGAC 2 cut(s) 363, 730
Bpu14I TTCGAA 1 cut(s) 804
BpuEI CTTGAG 4 cut(s) 253, 895, 1281, 1792
BpuMI CCSGG 1 cut(s) 1431
Bsa29I ATCGAT 1 cut(s) 1649
BsaAI YACGTR 1 cut(s) 677
BsaJI CCNNGG 2 cut(s) 790, 1826
Bsc4I CCNNNNNNNGG 1 cut(s) 862
Bse118I RCCGGY 1 cut(s) 41
Bse1I ACTGG 3 cut(s) 61, 339, 1456
Bse3DI GCAATG 3 cut(s) 399, 975, 1542
BseBI CCWGG 2 cut(s) 716, 792
BseCI ATCGAT 1 cut(s) 1649
BseDI CCNNGG 2 cut(s) 790, 1826
BseGI GGATG 2 cut(s) 1356, 1554
BseLI CCNNNNNNNGG 1 cut(s) 862
BseMI GCAATG 3 cut(s) 399, 975, 1542
BseMII CTCAG 2 cut(s) 245, 1425
BseNI ACTGG 3 cut(s) 61, 339, 1456
BseXI GCAGC 9 cut(s) 47, 311, 391, 394, 493, 756, 950, 1138, 1402
Bsh1236I CGCG 1 cut(s) 1567
Bsh1285I CGRYCG 1 cut(s) 586
BshFI GGCC 2 cut(s) 635, 704
BshNI GGYRCC 1 cut(s) 490
BshVI ATCGAT 1 cut(s) 1649
BsiEI CGRYCG 1 cut(s) 586
BsiHKAI GWGCWC 1 cut(s) 983
BsiHKCI CYCGRG 1 cut(s) 989
BsiSI CCGG 3 cut(s) 42, 303, 1430
BslFI GGGAC 1 cut(s) 1724
BslI CCNNNNNNNGG 1 cut(s) 862
BsmFI GGGAC 1 cut(s) 1724
BsmI GAATGC 1 cut(s) 812
BsnI GGCC 2 cut(s) 635, 704
BsoBI CYCGRG 1 cut(s) 989
Bsp119I TTCGAA 1 cut(s) 804
Bsp1286I GDGCHC 1 cut(s) 983
Bsp143I GATC 3 cut(s) 416, 1123, 1444
BspACI CCGC 8 cut(s) 218, 221, 410, 477, 741, 941, 1567, 1671
BspANI GGCC 2 cut(s) 635, 704
BspCNI CTCAG 2 cut(s) 246, 1424
BspDI ATCGAT 1 cut(s) 1649
BspFNI CGCG 1 cut(s) 1567
BspLI GGNNCC 2 cut(s) 492, 838
BspMAI CTGCAG 2 cut(s) 37, 372
BspMI ACCTGC 1 cut(s) 40
BspPI GGATC 1 cut(s) 424
BspQI GCTCTTC 1 cut(s) 1308
BspT104I TTCGAA 1 cut(s) 804
BspT107I GGYRCC 1 cut(s) 490
BspTI CTTAAG 1 cut(s) 167
BsrDI GCAATG 3 cut(s) 399, 975, 1542
BsrFI RCCGGY 1 cut(s) 41
BsrI ACTGG 3 cut(s) 61, 339, 1456
BssAI RCCGGY 1 cut(s) 41
BssECI CCNNGG 2 cut(s) 790, 1826
BssMI GATC 3 cut(s) 416, 1123, 1444
BssSI CACGAG 2 cut(s) 1184, 1589
Bst2BI CACGAG 2 cut(s) 1184, 1589
Bst2UI CCWGG 2 cut(s) 716, 792
Bst4CI ACNGT 4 cut(s) 286, 522, 1324, 1402
Bst6I CTCTTC 4 cut(s) 1308, 1531, 1637, 1798
BstAFI CTTAAG 1 cut(s) 167
BstBAI YACGTR 1 cut(s) 677
BstBI TTCGAA 1 cut(s) 804
BstC8I GCNNGC 3 cut(s) 195, 262, 1376
BstDEI CTNAG 2 cut(s) 254, 1411
BstDSI CCRYGG 1 cut(s) 1826
BstF5I GGATG 2 cut(s) 1356, 1554
BstFNI CGCG 1 cut(s) 1567
BstKTI GATC 3 cut(s) 419, 1126, 1447
BstMBI GATC 3 cut(s) 416, 1123, 1444
BstMCI CGRYCG 1 cut(s) 586
BstMWI GCNNNNNNNGC 6 cut(s) 107, 410, 503, 836, 1160, 1754
BstNI CCWGG 2 cut(s) 716, 792
BstNSI RCATGY 8 cut(s) 26, 97, 177, 197, 537, 1378, 1789, 1840
BstPAI GACNNNNGTC 1 cut(s) 127
BstSCI CCNGG 3 cut(s) 714, 790, 1429
BstSFI CTRYAG 3 cut(s) 33, 368, 1641
BstUI CGCG 1 cut(s) 1567
BstV1I GCAGC 9 cut(s) 47, 311, 391, 394, 493, 756, 950, 1138, 1402
BstV2I GAAGAC 2 cut(s) 363, 730
BstX2I RGATCY 1 cut(s) 416
BstXI CCANNNNNNTGG 1 cut(s) 762
BstYI RGATCY 1 cut(s) 416
Bsu15I ATCGAT 1 cut(s) 1649
BsuI GTATCC 1 cut(s) 235
BsuRI GGCC 2 cut(s) 635, 704
BsuTUI ATCGAT 1 cut(s) 1649
BtgI CCRYGG 1 cut(s) 1826
BtrI CACGTC 1 cut(s) 895
BtsCI GGATG 2 cut(s) 1356, 1554
BtsI GCAGTG 3 cut(s) 365, 387, 1359
BtsIMutI CAGTG 4 cut(s) 365, 387, 1329, 1359
BveI ACCTGC 1 cut(s) 40
Cac8I GCNNGC 3 cut(s) 195, 262, 1376
CaiI CAGNNNCTG 1 cut(s) 56
Cfr10I RCCGGY 1 cut(s) 41
ClaI ATCGAT 1 cut(s) 1649
Csp6I GTAC 3 cut(s) 143, 625, 678
CviQI GTAC 3 cut(s) 143, 625, 678
DdeI CTNAG 2 cut(s) 254, 1411
DpnI GATC 3 cut(s) 418, 1125, 1446
DpnII GATC 3 cut(s) 416, 1123, 1444
DraIII CACNNNGTG 1 cut(s) 325
Eam1104I CTCTTC 4 cut(s) 1308, 1531, 1637, 1798
EarI CTCTTC 4 cut(s) 1308, 1531, 1637, 1798
EciI GGCGGA 1 cut(s) 236
Ecl136II GAGCTC 1 cut(s) 981
Eco24I GRGCYC 1 cut(s) 983
Eco53kI GAGCTC 1 cut(s) 981
Eco57I CTGAAG 2 cut(s) 714, 1332
Eco88I CYCGRG 1 cut(s) 989
EcoICRI GAGCTC 1 cut(s) 981
EcoRII CCWGG 2 cut(s) 714, 790
EcoT22I ATGCAT 2 cut(s) 1212, 1376
EcoT38I GRGCYC 1 cut(s) 983
FalI AAGNNNNNCTT 2 cut(s) 150, 182
FaqI GGGAC 1 cut(s) 1724
FauNDI CATATG 2 cut(s) 1114, 1146
FbaI TGATCA 2 cut(s) 1123, 1444
FblI GTMKAC 1 cut(s) 1813
FokI GGATG 2 cut(s) 1363, 1541
FriOI GRGCYC 1 cut(s) 983
HaeIII GGCC 2 cut(s) 635, 704
HapII CCGG 3 cut(s) 42, 303, 1430
HinfI GANTC 7 cut(s) 47, 104, 228, 771, 1180, 1620, 1697
HpaII CCGG 3 cut(s) 42, 303, 1430
HphI GGTGA 2 cut(s) 21, 76
Hpy166II GTNNAC 5 cut(s) 686, 755, 765, 853, 1814
Hpy188I TCNGA 4 cut(s) 255, 526, 1023, 1783
Hpy8I GTNNAC 5 cut(s) 686, 755, 765, 853, 1814
HpyAV CCTTC 8 cut(s) 299, 664, 738, 927, 1277, 1331, 1348, 1684
HpyCH4III ACNGT 4 cut(s) 286, 522, 1324, 1402
HpyCH4IV ACGT 3 cut(s) 294, 676, 894
HpyF10VI GCNNNNNNNGC 6 cut(s) 107, 410, 503, 836, 1160, 1754
HpyF3I CTNAG 2 cut(s) 254, 1411
HpySE526I ACGT 3 cut(s) 294, 676, 894
Ksp22I TGATCA 2 cut(s) 1123, 1444
Kzo9I GATC 3 cut(s) 416, 1123, 1444
LguI GCTCTTC 1 cut(s) 1308
LmnI GCTCC 1 cut(s) 836
Lsp1109I GCAGC 9 cut(s) 47, 311, 391, 394, 493, 756, 950, 1138, 1402
LweI GCATC 5 cut(s) 640, 925, 1057, 1219, 1655
MaeII ACGT 3 cut(s) 294, 676, 894
MaeIII GTNAC 4 cut(s) 536, 1052, 1108, 1130
MalI GATC 3 cut(s) 418, 1125, 1446
MboI GATC 3 cut(s) 416, 1123, 1444
MfeI CAATTG 2 cut(s) 616, 1743
MflI RGATCY 1 cut(s) 416
MhlI GDGCHC 1 cut(s) 983
MlyI GAGTC 2 cut(s) 765, 1614
MmeI TCCRAC 4 cut(s) 525, 552, 1276, 1806
Mph1103I ATGCAT 2 cut(s) 1212, 1376
MroXI GAANNNNTTC 1 cut(s) 727
MseI TTAA 3 cut(s) 168, 777, 1328
MslI CAYNNNNRTG 3 cut(s) 92, 760, 1268
MspA1I CMGCKG 3 cut(s) 101, 941, 1415
MspCI CTTAAG 1 cut(s) 167
MspI CCGG 3 cut(s) 42, 303, 1430
MspR9I CCNGG 3 cut(s) 716, 792, 1431
MunI CAATTG 2 cut(s) 616, 1743
Mva1269I GAATGC 1 cut(s) 812
MvaI CCWGG 2 cut(s) 716, 792
MvnI CGCG 1 cut(s) 1567
MwoI GCNNNNNNNGC 6 cut(s) 107, 410, 503, 836, 1160, 1754
NciI CCSGG 1 cut(s) 1431
NdeI CATATG 2 cut(s) 1114, 1146
NdeII GATC 3 cut(s) 416, 1123, 1444
NlaIV GGNNCC 2 cut(s) 492, 838
NmuCI GTSAC 2 cut(s) 1052, 1108
NsiI ATGCAT 2 cut(s) 1212, 1376
NspI RCATGY 8 cut(s) 26, 97, 177, 197, 537, 1378, 1789, 1840
NspV TTCGAA 1 cut(s) 804
OliI CACNNNNGTG 1 cut(s) 760
PaeI GCATGC 2 cut(s) 197, 1378
PaqCI CACCTGC 1 cut(s) 40
PciI ACATGT 4 cut(s) 22, 93, 173, 1785
PciSI GCTCTTC 1 cut(s) 1308
PcsI WCGNNNNNNNCGW 1 cut(s) 1191
PctI GAATGC 1 cut(s) 812
PdmI GAANNNNTTC 1 cut(s) 727
PfeI GAWTC 5 cut(s) 47, 104, 228, 1180, 1697
PflFI GACNNNGTC 1 cut(s) 428
PleI GAGTC 2 cut(s) 765, 1614
PpsI GAGTC 2 cut(s) 765, 1614
Ppu21I YACGTR 1 cut(s) 677
PscI ACATGT 4 cut(s) 22, 93, 173, 1785
PshAI GACNNNNGTC 1 cut(s) 127
Psp124BI GAGCTC 1 cut(s) 983
Psp6I CCWGG 2 cut(s) 714, 790
PspGI CCWGG 2 cut(s) 714, 790
PspN4I GGNNCC 2 cut(s) 492, 838
PstI CTGCAG 2 cut(s) 37, 372
PstNI CAGNNNCTG 1 cut(s) 56
PsuI RGATCY 1 cut(s) 416
PsyI GACNNNGTC 1 cut(s) 428
PvuII CAGCTG 2 cut(s) 101, 1415
RsaI GTAC 3 cut(s) 144, 626, 679
RsaNI GTAC 3 cut(s) 143, 625, 678
RseI CAYNNNNRTG 3 cut(s) 92, 760, 1268
SacI GAGCTC 1 cut(s) 983
SapI GCTCTTC 1 cut(s) 1308
SaqAI TTAA 3 cut(s) 168, 777, 1328
Sau3AI GATC 3 cut(s) 416, 1123, 1444
SchI GAGTC 2 cut(s) 765, 1614
ScrFI CCNGG 3 cut(s) 716, 792, 1431
SduI GDGCHC 1 cut(s) 983
SfaNI GCATC 5 cut(s) 640, 925, 1057, 1219, 1655
SfcI CTRYAG 3 cut(s) 33, 368, 1641
SfuI TTCGAA 1 cut(s) 804
SmiMI CAYNNNNRTG 3 cut(s) 92, 760, 1268
SmlI CTYRAG 5 cut(s) 167, 232, 874, 1296, 1807
SmoI CTYRAG 5 cut(s) 167, 232, 874, 1296, 1807
SpeI ACTAGT 2 cut(s) 139, 680
SphI GCATGC 2 cut(s) 197, 1378
SsiI CCGC 8 cut(s) 218, 221, 410, 477, 741, 941, 1567, 1671
SstI GAGCTC 1 cut(s) 983
StyD4I CCNGG 3 cut(s) 714, 790, 1429
TaaI ACNGT 4 cut(s) 286, 522, 1324, 1402
TaiI ACGT 3 cut(s) 297, 679, 897
TaqI TCGA 6 cut(s) 90, 721, 804, 1213, 1266, 1649
TaqII GACCGA 1 cut(s) 600
TatI WGTACW 1 cut(s) 142
TauI GCSGC 3 cut(s) 221, 413, 744
TfiI GAWTC 5 cut(s) 47, 104, 228, 1180, 1697
Tru1I TTAA 3 cut(s) 168, 777, 1328
Tru9I TTAA 3 cut(s) 168, 777, 1328
TscAI CASTG 4 cut(s) 372, 394, 1329, 1366
TseFI GTSAC 2 cut(s) 1052, 1108
TseI GCWGC 9 cut(s) 35, 299, 404, 407, 506, 744, 938, 1151, 1415
Tsp45I GTSAC 2 cut(s) 1052, 1108
TspRI CASTG 4 cut(s) 372, 394, 1329, 1366
Tth111I GACNNNGTC 1 cut(s) 428
Vha464I CTTAAG 1 cut(s) 167
XapI RAATTY 4 cut(s) 592, 922, 1714, 1766
XbaI TCTAGA 3 cut(s) 269, 421, 1486
XceI RCATGY 8 cut(s) 26, 97, 177, 197, 537, 1378, 1789, 1840
XmiI GTMKAC 1 cut(s) 1813
XmnI GAANNNNTTC 1 cut(s) 727
Zsp2I ATGCAT 2 cut(s) 1212, 1376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.