Rh5DG291500

Ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000157
Physical Location & Seq
Reverse (-)
36408000 .. 36410549
2550 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG291500.1

Sequence Viewer

Length: 1410 bp
ATGTCTATCCCACAAGCACAACATGTATCACCTTCCCAGATTCAATTAACCACAGCTAGCAGCGATTCAGTGACTGGGATAGACAACCAATCTGCAGACATCGCCATCCACATTTCAGCTGATCATTCGGCTCAACCTAACAGACCTTCGTCTTATTTACTAGAACATACAAACAGAGAAGAATCCCTTAAGACACGTGTTGCCCTCTATAAGTCTGCACTAAAAGGCGATTGGGAAGCTGCTGAACTAATCTTGAAAAAGGATAGGAGCAGACTTCTGCGAGCTAGCATATCTAGAGGATATGATACAGTCCTTCACGTTGCAGCAGGAACAAGCCATATTCACTTTGTGGAGAAACTGGTCAGCATGATGGAAGTAGAAGACTTAGCACTGCAGGACGTAAATGGAAACACAGCCTTGTGCATTGCTGCTTCGGCTGGATCTCTAGACATTGTCCAGATTTTGATACAAAAGAACAGGTCATTGCTAAAAATTAGAGGCGGTAAAGGTATGGCACCGGCCTATATGGCTGCTGTGTTAGGACAGTCCCAAATGGCATGTTACCTGTACCGGTACCGTGAAAGTAAAGACATGCTGGAGAAAACCGACCGAGAAAACTTGTTTTTTTCTTGCATCAACAATTGTCTTTACGATTTGGCCTTTCAGTTGCTAGATACTGATAAAGCATTAGCGAAGGCACGTACTAGTTCGCAGAAAAATAAAACGGCCTTGCATATCCTGGCTCGAAGACCTTCAGCATTTGGTGGCAGCCAAAGTCCACCAATGTGGACTAGACTCATCAACTCATTCCTCCCAGGTTTGAAGTTCGAATGCAAAAGAAAGTTGAGAGAAGCTAATGGAGCCTTTAAACTAGTCCACAGCCTCTGGGAAGAAATCTTGAGAGATGAGCATGATAACGTGATGAGGCTGATCAAATATCCTTCAAATTTATTATTTGATGCAGCGGAATTAGGGAATTATGAGTTTCTGGCAATGCTTATGAGCTCTTATCCTGAGTTAGTATGGGAAACTAATGAAAAAAATCGAACCATAATCCATGTCGCAGTTCTGCATCGTCACGCAAGTATCTTCAACCTAGTGCATGAAATAGGCTCAATCAAAGATGTCATCGTGACATATGAAGATGATCAGGGTAACAACATTGTGCATATGGCTGCAAAATTAGCACCTCAAAATCAATTGAATCTCGAGTCAGGCGTGGCTCTTCAAATGCATCGAGAGTTGGTATGGTTTGAGGAAGTCAAAAAGATCGTCCAACCTCAATACATCGAGATGGAAAATAACGAAGGAAAAACACCTCAAGAATTATTTACTGAAGAGCATATGTCTACTATTTCAGCCGTGGAAACACATGCTCTACTAGAAGTGGAGCTGAATGCTGTACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

469

Amino Acids

52.77

Weight (kDa)

6.15

Isoelectric Point (pI)

46.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 105 - 192 4.8e-10 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54070 AT5G35830
fragaria_vesca FvH4_3g25070 FvH4_3g25080 FvH4_3g25081 FvH4_3g25081
malus_domestica MD03G1027500.v1.1 MD03G1146300.v1.1 MD03G1146800.v1.1 MD03G1146900.v1.1 MD03G1147100.v1.1 MD11G1165600.v1.1
prunus_persica Prupe.2G015800_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G016300_v2.0.a1 Prupe.2G016500_v2.0.a1 Prupe.6G131100_v2.0.a1 Prupe.6G131400_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1
pyrus_communis pycom03g10090 pycom03g10110 pycom11g13740 pycom11g13770
rosa_chinensis RchiOBHm_Chr1g0316371 RchiOBHm_Chr1g0316381 RchiOBHm_Chr1g0316401 RchiOBHm_Chr1g0316411 RchiOBHm_Chr1g0316571 RchiOBHm_Chr1g0316601 RchiOBHm_Chr5g0039341 RchiOBHm_Chr5g0041371 RchiOBHm_Chr5g0041401 RchiOBHm_Chr5g0041431 RchiOBHm_Chr5g0041471 RchiOBHm_Chr5g0045281 RchiOBHm_Chr5g0045291 RchiOBHm_Chr5g0045311 RchiOBHm_Chr5g0045371 RchiOBHm_Chr5g0045421 RchiOBHm_Chr5g0045431 RchiOBHm_Chr5g0045501 RchiOBHm_Chr5g0045551 RchiOBHm_Chr5g0045771 RchiOBHm_Chr5g0045811
rosa_laevigata RLG00000034057 RLG00000034058
rosa_multiflora Rmu_co8352751.1_g000001 Rmu_co8397575.1_g000001 Rmu_sc0000415.1_g000006 Rmu_sc0000493.1_g000057 Rmu_sc0000504.1_g000003 Rmu_sc0000594.1_g000019 Rmu_sc0000594.1_g000032 Rmu_sc0000594.1_g000066 Rmu_sc0001047.1_g000003 Rmu_sc0001047.1_g000011 Rmu_sc0001556.1_g000023 Rmu_sc0002584.1_g000003 Rmu_sc0002584.1_g000039 Rmu_sc0002584.1_g000070 Rmu_sc0002667.1_g000015 Rmu_sc0003677.1_g000003 Rmu_sc0003743.1_g000017 Rmu_sc0004520.1_g000004 Rmu_sc0004520.1_g000013 Rmu_sc0004520.1_g000027 Rmu_sc0005539.1_g000014 Rmu_sc0005539.1_g000032 Rmu_sc0006018.1_g000006 Rmu_sc0006737.1_g000020 Rmu_sc0007485.1_g000002 Rmu_sc0008729.1_g000002 Rmu_sc0008729.1_g000003 Rmu_sc0038822.1_g000001 Rmu_ssc0000398.1_g000025
rosa_roxburghii Rroxscaffold_1G00035090 Rroxscaffold_1G00035630 Rroxscaffold_1G00035640 Rroxscaffold_1G00035660 Rroxscaffold_1G00035680 Rroxscaffold_1G00035730 Rroxscaffold_1G00035750 Rroxscaffold_1G00035770 Rroxscaffold_1G00039150 Rroxscaffold_1G00039190 Rroxscaffold_1G00039220 Rroxscaffold_1G00039260 Rroxscaffold_4G00328910 Rroxscaffold_4G00328950 Rroxscaffold_4G00328960 Rroxscaffold_4G00330890
rosa_rugosa Rorug01G0009800 Rorug01G0010000.1 Rorug01G0010200 Rorug01G0012100 Rorug01G0023500 Rorug01G0023600 Rorug01G0023700 Rorug01G0024700 Rorug05G0192000 Rorug05G0192200 Rorug05G0222400 Rorug05G0222500 Rorug05G0222600.1 Rorug05G0222700.1 Rorug05G0222800.1 Rorug05G0222900.1 Rorug05G0223000.1 Rorug05G0223100.1 Rorug05G0223200 Rorug05G0223600 Rorug05G0226800 Rorug05G0226900
rosa_samantha Rh1CG019600 Rh1DG015000 Rh1DG015300 Rh1DG016900 Rh5CG315200 Rh5CG338700 Rh5DG277100 Rh5DG291500 Rh5DG292100 Rh5DG292200 Rh5DG292400 Rh5DG292600 Rh5DG322100 Rh5DG322200 Rh5DG323100 Rh5DG323300 Rh5DG326700 Rh5DG326900 Rh5DG327000
rosa_wichuraiana Rw0G021580 Rw1G001320 Rw1G001420 Rw1G002820 Rw1G002840 Rw1G002870 Rw5G024800 Rw5G026160 Rw5G026170 Rw5G026180 Rw5G026210 Rw5G026230 Rw5G028170 Rw5G028190 Rw5G028210 Rw5G028230 Rw5G028240 Rw5G028260 Rw5G028280 Rw5G028370 Rw5G028380 Rw5G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 573
AccB1I GGYRCC 2 cut(s) 514, 573
AccI GTMKAC 1 cut(s) 1349
AciI CCGC 2 cut(s) 501, 965
AclWI GGATC 1 cut(s) 448
AcsI RAATTY 1 cut(s) 946
AcuI CTGAAG 2 cut(s) 738, 1356
AcvI CACGTG 1 cut(s) 197
AdeI CACNNNGTG 1 cut(s) 349
AfaI GTAC 4 cut(s) 569, 575, 703, 1404
AflII CTTAAG 1 cut(s) 188
AflIII ACRYGT 3 cut(s) 22, 194, 196
AgeI ACCGGT 1 cut(s) 570
AgsI TTSAA 7 cut(s) 44, 256, 823, 945, 1093, 1204, 1229
AhlI ACTAGT 2 cut(s) 704, 871
AjnI CCWGG 2 cut(s) 738, 814
AleI CACNNNNGTG 1 cut(s) 784
AluBI AGCT 7 cut(s) 56, 119, 239, 284, 854, 1005, 1393
AluI AGCT 7 cut(s) 56, 119, 239, 284, 854, 1005, 1393
Alw21I GWGCWC 1 cut(s) 1007
AlwI GGATC 1 cut(s) 448
AlwNI CAGNNNCTG 2 cut(s) 74, 885
Ama87I CYCGRG 1 cut(s) 1208
AoxI GGCC 3 cut(s) 519, 657, 726
ApeKI GCWGC 8 cut(s) 60, 239, 323, 428, 530, 768, 962, 1175
ApoI RAATTY 1 cut(s) 946
ArsI GACNNNNNNTTYG 2 cut(s) 1257, 1289
AsiGI ACCGGT 1 cut(s) 570
Asp700I GAANNNNTTC 1 cut(s) 751
Asp718I GGTACC 1 cut(s) 573
AsuHPI GGTGA 1 cut(s) 21
AsuII TTCGAA 1 cut(s) 828
AsuNHI GCTAGC 2 cut(s) 56, 284
AvaI CYCGRG 1 cut(s) 1208
BaeI ACNNNNGTAYC 2 cut(s) 1069, 1102
BanI GGYRCC 2 cut(s) 514, 573
BanII GRGCYC 1 cut(s) 1007
BbrPI CACGTG 1 cut(s) 197
BbsI GAAGAC 2 cut(s) 387, 754
Bbv12I GWGCWC 1 cut(s) 1007
BbvI GCAGC 8 cut(s) 72, 226, 335, 415, 517, 780, 974, 1162
BccI CCATC 3 cut(s) 113, 364, 1288
BceAI ACGGC 2 cut(s) 741, 1346
BciT130I CCWGG 2 cut(s) 740, 816
BclI TGATCA 3 cut(s) 121, 930, 1147
BcuI ACTAGT 2 cut(s) 704, 871
BfmI CTRYAG 2 cut(s) 93, 392
BfrI CTTAAG 1 cut(s) 188
BglI GCCNNNNNGGC 1 cut(s) 527
BisI GCNGC 8 cut(s) 61, 240, 324, 429, 531, 769, 963, 1176
BlsI GCNGC 8 cut(s) 62, 241, 325, 430, 532, 770, 964, 1177
Bme1390I CCNGG 2 cut(s) 740, 816
BmeT110I CYCGRG 1 cut(s) 1208
BmiI GGNNCC 3 cut(s) 516, 575, 862
BmrFI CCNGG 2 cut(s) 740, 816
BmrI ACTGGG 1 cut(s) 84
BmsI GCATC 4 cut(s) 642, 949, 1081, 1243
BmtI GCTAGC 2 cut(s) 60, 288
BmuI ACTGGG 1 cut(s) 84
BoxI GACNNNNGTC 1 cut(s) 148
BpiI GAAGAC 2 cut(s) 387, 754
BpmI CTGGAG 1 cut(s) 617
Bpu14I TTCGAA 1 cut(s) 828
BpuEI CTTGAG 2 cut(s) 919, 1305
BsaAI YACGTR 2 cut(s) 197, 701
BsaJI CCNNGG 2 cut(s) 814, 1362
BsaWI WCCGGW 1 cut(s) 570
Bse118I RCCGGY 2 cut(s) 517, 570
Bse1I ACTGG 2 cut(s) 79, 363
Bse3DI GCAATG 3 cut(s) 423, 482, 999
BseBI CCWGG 2 cut(s) 740, 816
BseDI CCNNGG 2 cut(s) 814, 1362
BseGI GGATG 1 cut(s) 105
BseMI GCAATG 3 cut(s) 423, 482, 999
BseMII CTCAG 1 cut(s) 1005
BseNI ACTGG 2 cut(s) 79, 363
BseXI GCAGC 8 cut(s) 72, 226, 335, 415, 517, 780, 974, 1162
BsgI GTGCAG 1 cut(s) 201
Bsh1285I CGRYCG 1 cut(s) 610
BshFI GGCC 3 cut(s) 521, 659, 728
BshNI GGYRCC 2 cut(s) 514, 573
BshTI ACCGGT 1 cut(s) 570
BsiEI CGRYCG 1 cut(s) 610
BsiHKAI GWGCWC 1 cut(s) 1007
BsiHKCI CYCGRG 1 cut(s) 1208
BsiSI CCGG 2 cut(s) 518, 571
BslFI GGGAC 1 cut(s) 532
BsmFI GGGAC 1 cut(s) 532
BsmI GAATGC 2 cut(s) 836, 1402
BsnI GGCC 3 cut(s) 521, 659, 728
BsoBI CYCGRG 1 cut(s) 1208
Bsp119I TTCGAA 1 cut(s) 828
Bsp1286I GDGCHC 1 cut(s) 1007
Bsp1407I TGTACA 1 cut(s) 1402
Bsp143I GATC 5 cut(s) 121, 440, 930, 1147, 1269
BspACI CCGC 2 cut(s) 501, 965
BspANI GGCC 3 cut(s) 521, 659, 728
BspCNI CTCAG 1 cut(s) 1006
BspLI GGNNCC 3 cut(s) 516, 575, 862
BspMAI CTGCAG 2 cut(s) 97, 396
BspOI GCTAGC 2 cut(s) 60, 288
BspPI GGATC 1 cut(s) 448
BspQI GCTCTTC 2 cut(s) 1230, 1332
BspT104I TTCGAA 1 cut(s) 828
BspT107I GGYRCC 2 cut(s) 514, 573
BspTI CTTAAG 1 cut(s) 188
BsrDI GCAATG 3 cut(s) 423, 482, 999
BsrFI RCCGGY 2 cut(s) 517, 570
BsrGI TGTACA 1 cut(s) 1402
BsrI ACTGG 2 cut(s) 79, 363
BssAI RCCGGY 2 cut(s) 517, 570
BssECI CCNNGG 2 cut(s) 814, 1362
BssMI GATC 5 cut(s) 121, 440, 930, 1147, 1269
Bst2UI CCWGG 2 cut(s) 740, 816
Bst4CI ACNGT 3 cut(s) 310, 546, 578
Bst6I CTCTTC 2 cut(s) 1230, 1332
BstAFI CTTAAG 1 cut(s) 188
BstAUI TGTACA 1 cut(s) 1402
BstBAI YACGTR 2 cut(s) 197, 701
BstBI TTCGAA 1 cut(s) 828
BstC8I GCNNGC 3 cut(s) 58, 282, 286
BstDEI CTNAG 2 cut(s) 385, 1014
BstDSI CCRYGG 1 cut(s) 1362
BstF5I GGATG 1 cut(s) 105
BstKTI GATC 5 cut(s) 124, 443, 933, 1150, 1272
BstMBI GATC 5 cut(s) 121, 440, 930, 1147, 1269
BstMCI CGRYCG 1 cut(s) 610
BstMWI GCNNNNNNNGC 5 cut(s) 101, 434, 527, 860, 1184
BstNI CCWGG 2 cut(s) 740, 816
BstNSI RCATGY 4 cut(s) 26, 561, 595, 1376
BstPAI GACNNNNGTC 1 cut(s) 148
BstSCI CCNGG 2 cut(s) 738, 814
BstSFI CTRYAG 2 cut(s) 93, 392
BstV1I GCAGC 8 cut(s) 72, 226, 335, 415, 517, 780, 974, 1162
BstV2I GAAGAC 2 cut(s) 387, 754
BstX2I RGATCY 1 cut(s) 440
BstXI CCANNNNNNTGG 1 cut(s) 786
BstYI RGATCY 1 cut(s) 440
BsuRI GGCC 3 cut(s) 521, 659, 728
BtgI CCRYGG 1 cut(s) 1362
BtgZI GCGATG 1 cut(s) 85
BtsCI GGATG 1 cut(s) 105
BtsI GCAGTG 1 cut(s) 389
BtsIMutI CAGTG 2 cut(s) 75, 389
Cac8I GCNNGC 3 cut(s) 58, 282, 286
CaiI CAGNNNCTG 2 cut(s) 74, 885
Cfr10I RCCGGY 2 cut(s) 517, 570
Csp6I GTAC 4 cut(s) 568, 574, 702, 1403
CspAI ACCGGT 1 cut(s) 570
CviAII CATG 8 cut(s) 23, 367, 558, 592, 911, 1058, 1103, 1373
CviQI GTAC 4 cut(s) 568, 574, 702, 1403
DdeI CTNAG 2 cut(s) 385, 1014
DpnI GATC 5 cut(s) 123, 442, 932, 1149, 1271
DpnII GATC 5 cut(s) 121, 440, 930, 1147, 1269
DraI TTTAAA 1 cut(s) 868
DraIII CACNNNGTG 1 cut(s) 349
Eam1104I CTCTTC 2 cut(s) 1230, 1332
EarI CTCTTC 2 cut(s) 1230, 1332
Ecl136II GAGCTC 1 cut(s) 1005
Eco24I GRGCYC 1 cut(s) 1007
Eco53kI GAGCTC 1 cut(s) 1005
Eco57I CTGAAG 2 cut(s) 738, 1356
Eco72I CACGTG 1 cut(s) 197
Eco88I CYCGRG 1 cut(s) 1208
EcoICRI GAGCTC 1 cut(s) 1005
EcoRII CCWGG 2 cut(s) 738, 814
EcoT22I ATGCAT 1 cut(s) 1236
EcoT38I GRGCYC 1 cut(s) 1007
FaeI CATG 8 cut(s) 26, 370, 561, 595, 914, 1061, 1106, 1376
FalI AAGNNNNNCTT 2 cut(s) 171, 203
FaqI GGGAC 1 cut(s) 532
FatI CATG 8 cut(s) 22, 366, 557, 591, 910, 1057, 1102, 1372
FauNDI CATATG 3 cut(s) 1138, 1170, 1344
FbaI TGATCA 3 cut(s) 121, 930, 1147
FblI GTMKAC 1 cut(s) 1349
Fnu4HI GCNGC 8 cut(s) 61, 240, 324, 429, 531, 769, 963, 1176
FokI GGATG 1 cut(s) 92
FriOI GRGCYC 1 cut(s) 1007
Fsp4HI GCNGC 8 cut(s) 61, 240, 324, 429, 531, 769, 963, 1176
GluI GCNGC 8 cut(s) 61, 240, 324, 429, 531, 769, 963, 1176
GsuI CTGGAG 1 cut(s) 617
HaeIII GGCC 3 cut(s) 521, 659, 728
HapII CCGG 2 cut(s) 518, 571
Hin1II CATG 8 cut(s) 26, 370, 561, 595, 914, 1061, 1106, 1376
HinfI GANTC 6 cut(s) 40, 65, 182, 795, 1204, 1211
HpaII CCGG 2 cut(s) 518, 571
HphI GGTGA 1 cut(s) 21
Hpy166II GTNNAC 4 cut(s) 779, 789, 877, 1350
Hpy8I GTNNAC 4 cut(s) 779, 789, 877, 1350
HpyAV CCTTC 7 cut(s) 42, 156, 323, 688, 762, 951, 1301
HpyCH4III ACNGT 3 cut(s) 310, 546, 578
HpyCH4IV ACGT 5 cut(s) 196, 318, 399, 700, 918
HpyF10VI GCNNNNNNNGC 5 cut(s) 101, 434, 527, 860, 1184
HpyF3I CTNAG 2 cut(s) 385, 1014
HpySE526I ACGT 5 cut(s) 196, 318, 399, 700, 918
Hsp92II CATG 8 cut(s) 26, 370, 561, 595, 914, 1061, 1106, 1376
KpnI GGTACC 1 cut(s) 577
Ksp22I TGATCA 3 cut(s) 121, 930, 1147
Kzo9I GATC 5 cut(s) 121, 440, 930, 1147, 1269
LguI GCTCTTC 2 cut(s) 1230, 1332
LmnI GCTCC 3 cut(s) 267, 860, 1390
Lsp1109I GCAGC 8 cut(s) 72, 226, 335, 415, 517, 780, 974, 1162
LweI GCATC 4 cut(s) 642, 949, 1081, 1243
MaeII ACGT 5 cut(s) 196, 318, 399, 700, 918
MaeIII GTNAC 5 cut(s) 70, 560, 1076, 1132, 1154
MalI GATC 5 cut(s) 123, 442, 932, 1149, 1271
MboI GATC 5 cut(s) 121, 440, 930, 1147, 1269
MboII GAAGA 8 cut(s) 191, 392, 759, 902, 1081, 1154, 1217, 1349
MfeI CAATTG 2 cut(s) 640, 1199
MflI RGATCY 1 cut(s) 440
MhlI GDGCHC 1 cut(s) 1007
MluCI AATT 9 cut(s) 44, 492, 640, 946, 968, 976, 1181, 1199, 1325
MlyI GAGTC 2 cut(s) 789, 1220
MmeI TCCRAC 1 cut(s) 1300
Mph1103I ATGCAT 1 cut(s) 1236
MroXI GAANNNNTTC 1 cut(s) 751
MseI TTAA 3 cut(s) 47, 189, 867
MslI CAYNNNNRTG 2 cut(s) 784, 1292
MspA1I CMGCKG 2 cut(s) 119, 965
MspCI CTTAAG 1 cut(s) 188
MspI CCGG 2 cut(s) 518, 571
MspR9I CCNGG 2 cut(s) 740, 816
MunI CAATTG 2 cut(s) 640, 1199
Mva1269I GAATGC 2 cut(s) 836, 1402
MvaI CCWGG 2 cut(s) 740, 816
MwoI GCNNNNNNNGC 5 cut(s) 101, 434, 527, 860, 1184
NdeI CATATG 3 cut(s) 1138, 1170, 1344
NdeII GATC 5 cut(s) 121, 440, 930, 1147, 1269
NheI GCTAGC 2 cut(s) 56, 284
NlaIII CATG 8 cut(s) 26, 370, 561, 595, 914, 1061, 1106, 1376
NlaIV GGNNCC 3 cut(s) 516, 575, 862
NmuCI GTSAC 3 cut(s) 70, 1076, 1132
NsiI ATGCAT 1 cut(s) 1236
NspI RCATGY 4 cut(s) 26, 561, 595, 1376
NspV TTCGAA 1 cut(s) 828
OliI CACNNNNGTG 1 cut(s) 784
PaeR7I CTCGAG 1 cut(s) 1208
PciI ACATGT 1 cut(s) 22
PciSI GCTCTTC 2 cut(s) 1230, 1332
PcsI WCGNNNNNNNCGW 1 cut(s) 1215
PctI GAATGC 2 cut(s) 836, 1402
PdmI GAANNNNTTC 1 cut(s) 751
PfeI GAWTC 4 cut(s) 40, 65, 182, 1204
PflFI GACNNNGTC 1 cut(s) 452
PinAI ACCGGT 1 cut(s) 570
PkrI GCNGC 8 cut(s) 62, 241, 325, 430, 532, 770, 964, 1177
PleI GAGTC 2 cut(s) 789, 1219
PmaCI CACGTG 1 cut(s) 197
PmlI CACGTG 1 cut(s) 197
PpsI GAGTC 2 cut(s) 789, 1219
Ppu21I YACGTR 2 cut(s) 197, 701
PscI ACATGT 1 cut(s) 22
PshAI GACNNNNGTC 1 cut(s) 148
Psp124BI GAGCTC 1 cut(s) 1007
Psp6I CCWGG 2 cut(s) 738, 814
PspCI CACGTG 1 cut(s) 197
PspGI CCWGG 2 cut(s) 738, 814
PspN4I GGNNCC 3 cut(s) 516, 575, 862
PstI CTGCAG 2 cut(s) 97, 396
PstNI CAGNNNCTG 2 cut(s) 74, 885
PsuI RGATCY 1 cut(s) 440
PsyI GACNNNGTC 1 cut(s) 452
PvuII CAGCTG 1 cut(s) 119
RsaI GTAC 4 cut(s) 569, 575, 703, 1404
RsaNI GTAC 4 cut(s) 568, 574, 702, 1403
RseI CAYNNNNRTG 2 cut(s) 784, 1292
SacI GAGCTC 1 cut(s) 1007
SapI GCTCTTC 2 cut(s) 1230, 1332
SaqAI TTAA 3 cut(s) 47, 189, 867
SatI GCNGC 8 cut(s) 61, 240, 324, 429, 531, 769, 963, 1176
Sau3AI GATC 5 cut(s) 121, 440, 930, 1147, 1269
SchI GAGTC 2 cut(s) 789, 1220
ScrFI CCNGG 2 cut(s) 740, 816
SduI GDGCHC 1 cut(s) 1007
SfaNI GCATC 4 cut(s) 642, 949, 1081, 1243
SfcI CTRYAG 2 cut(s) 93, 392
Sfr274I CTCGAG 1 cut(s) 1208
SfuI TTCGAA 1 cut(s) 828
SlaI CTCGAG 1 cut(s) 1208
SmiMI CAYNNNNRTG 2 cut(s) 784, 1292
SmlI CTYRAG 4 cut(s) 188, 898, 1208, 1320
SmoI CTYRAG 4 cut(s) 188, 898, 1208, 1320
SpeI ACTAGT 2 cut(s) 704, 871
Sse9I AATT 9 cut(s) 44, 492, 640, 946, 968, 976, 1181, 1199, 1325
SsiI CCGC 2 cut(s) 501, 965
SstI GAGCTC 1 cut(s) 1007
StyD4I CCNGG 2 cut(s) 738, 814
TaaI ACNGT 3 cut(s) 310, 546, 578
TaiI ACGT 5 cut(s) 199, 321, 402, 703, 921
TaqI TCGA 6 cut(s) 745, 828, 1045, 1209, 1237, 1290
TaqII GACCGA 1 cut(s) 624
TasI AATT 9 cut(s) 44, 492, 640, 946, 968, 976, 1181, 1199, 1325
TatI WGTACW 1 cut(s) 1402
TfiI GAWTC 4 cut(s) 40, 65, 182, 1204
Tru1I TTAA 3 cut(s) 47, 189, 867
Tru9I TTAA 3 cut(s) 47, 189, 867
TscAI CASTG 2 cut(s) 75, 396
TseFI GTSAC 3 cut(s) 70, 1076, 1132
TseI GCWGC 8 cut(s) 60, 239, 323, 428, 530, 768, 962, 1175
Tsp45I GTSAC 3 cut(s) 70, 1076, 1132
TspDTI ATGAA 3 cut(s) 1050, 1119, 1155
TspRI CASTG 2 cut(s) 75, 396
Tth111I GACNNNGTC 1 cut(s) 452
Vha464I CTTAAG 1 cut(s) 188
XapI RAATTY 1 cut(s) 946
XbaI TCTAGA 2 cut(s) 293, 445
XceI RCATGY 4 cut(s) 26, 561, 595, 1376
XhoI CTCGAG 1 cut(s) 1208
XmiI GTMKAC 1 cut(s) 1349
XmnI GAANNNNTTC 1 cut(s) 751
Zsp2I ATGCAT 1 cut(s) 1236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.