Rroxscaffold_4G00328960

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000157
Physical Location & Seq
Forward (+)
62086201 .. 62086779
579 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00328960.1

Sequence Viewer

Length: 579 bp
ATGGTCTCCCGCGAACATCTCAAGAAGGAAGCGCCGCACAAGTGGGCAGGACACCTCCACCCGGGTGGCGATTCACATGGTGAAGCCACTTCAGCTGATCAACAACGCAGTACATCTCAACATGGAAATGCAACAGAATCTCATGAAGACAAAACGCCTCAGGAGTTATTCACTATTCAGCATAAATTACTGCTGAAAGAAGGACGAAAGTGGATGAATGGCACAGCGCAATCATGTACAGTTGTATCAACTCTTATTGCTGGTGCTTTATTTGCTGCAGGGATCACTGTCACTCTTGGAGGAAATAAAGGAACTTCGGATCACAACTTGCAAAAAGTGGTTTATCGGATTTTCATCGTATCAGATGCAATGGCCTTTTTGTTTGCCTTGGCTGCAATACTAACCTTCTTGTCTATCCTTACTTCACGTTATGCTGAAAGAGATTTTCTTGAATCCTTACCTTCAAAGTTGAGAGTGGGACTTGGATTACTTTTCTGTTCTATTATAACGATGATGGTGGCATTCTGCTCGGCTTTCATTATTTCTTCCAGTGTTGAAGACTTACTCCCCCCTATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

192

Amino Acids

20.9

Weight (kDa)

7.13

Isoelectric Point (pI)

38.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGG PF13962 70 - 180 2.2e-19 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54070 AT5G35830
fragaria_vesca FvH4_3g25070 FvH4_3g25080 FvH4_3g25081 FvH4_3g25081
malus_domestica MD03G1027500.v1.1 MD03G1146300.v1.1 MD03G1146800.v1.1 MD03G1146900.v1.1 MD03G1147100.v1.1 MD11G1165600.v1.1
prunus_persica Prupe.2G015800_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G016300_v2.0.a1 Prupe.2G016500_v2.0.a1 Prupe.6G131100_v2.0.a1 Prupe.6G131400_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1
pyrus_communis pycom03g10090 pycom03g10110 pycom11g13740 pycom11g13770
rosa_chinensis RchiOBHm_Chr1g0316371 RchiOBHm_Chr1g0316381 RchiOBHm_Chr1g0316401 RchiOBHm_Chr1g0316411 RchiOBHm_Chr1g0316571 RchiOBHm_Chr1g0316601 RchiOBHm_Chr5g0039341 RchiOBHm_Chr5g0041371 RchiOBHm_Chr5g0041401 RchiOBHm_Chr5g0041431 RchiOBHm_Chr5g0041471 RchiOBHm_Chr5g0045281 RchiOBHm_Chr5g0045291 RchiOBHm_Chr5g0045311 RchiOBHm_Chr5g0045371 RchiOBHm_Chr5g0045421 RchiOBHm_Chr5g0045431 RchiOBHm_Chr5g0045501 RchiOBHm_Chr5g0045551 RchiOBHm_Chr5g0045771 RchiOBHm_Chr5g0045811
rosa_laevigata RLG00000034057 RLG00000034058
rosa_multiflora Rmu_co8352751.1_g000001 Rmu_co8397575.1_g000001 Rmu_sc0000415.1_g000006 Rmu_sc0000493.1_g000057 Rmu_sc0000504.1_g000003 Rmu_sc0000594.1_g000019 Rmu_sc0000594.1_g000032 Rmu_sc0000594.1_g000066 Rmu_sc0001047.1_g000003 Rmu_sc0001047.1_g000011 Rmu_sc0001556.1_g000023 Rmu_sc0002584.1_g000003 Rmu_sc0002584.1_g000039 Rmu_sc0002584.1_g000070 Rmu_sc0002667.1_g000015 Rmu_sc0003677.1_g000003 Rmu_sc0003743.1_g000017 Rmu_sc0004520.1_g000004 Rmu_sc0004520.1_g000013 Rmu_sc0004520.1_g000027 Rmu_sc0005539.1_g000014 Rmu_sc0005539.1_g000032 Rmu_sc0006018.1_g000006 Rmu_sc0006737.1_g000020 Rmu_sc0007485.1_g000002 Rmu_sc0008729.1_g000002 Rmu_sc0008729.1_g000003 Rmu_sc0038822.1_g000001 Rmu_ssc0000398.1_g000025
rosa_roxburghii Rroxscaffold_1G00035090 Rroxscaffold_1G00035630 Rroxscaffold_1G00035640 Rroxscaffold_1G00035660 Rroxscaffold_1G00035680 Rroxscaffold_1G00035730 Rroxscaffold_1G00035750 Rroxscaffold_1G00035770 Rroxscaffold_1G00039150 Rroxscaffold_1G00039190 Rroxscaffold_1G00039220 Rroxscaffold_1G00039260 Rroxscaffold_4G00328910 Rroxscaffold_4G00328950 Rroxscaffold_4G00328960 Rroxscaffold_4G00330890
rosa_rugosa Rorug01G0009800 Rorug01G0010000.1 Rorug01G0010200 Rorug01G0012100 Rorug01G0023500 Rorug01G0023600 Rorug01G0023700 Rorug01G0024700 Rorug05G0192000 Rorug05G0192200 Rorug05G0222400 Rorug05G0222500 Rorug05G0222600.1 Rorug05G0222700.1 Rorug05G0222800.1 Rorug05G0222900.1 Rorug05G0223000.1 Rorug05G0223100.1 Rorug05G0223200 Rorug05G0223600 Rorug05G0226800 Rorug05G0226900
rosa_samantha Rh1CG019600 Rh1DG015000 Rh1DG015300 Rh1DG016900 Rh5CG315200 Rh5CG338700 Rh5DG277100 Rh5DG291500 Rh5DG292100 Rh5DG292200 Rh5DG292400 Rh5DG292600 Rh5DG322100 Rh5DG322200 Rh5DG323100 Rh5DG323300 Rh5DG326700 Rh5DG326900 Rh5DG327000
rosa_wichuraiana Rw0G021580 Rw1G001320 Rw1G001420 Rw1G002820 Rw1G002840 Rw1G002870 Rw5G024800 Rw5G026160 Rw5G026170 Rw5G026180 Rw5G026210 Rw5G026230 Rw5G028170 Rw5G028190 Rw5G028210 Rw5G028230 Rw5G028240 Rw5G028260 Rw5G028280 Rw5G028370 Rw5G028380 Rw5G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 506
AccII CGCG 1 cut(s) 12
AciI CCGC 2 cut(s) 10, 35
AclWI GGATC 2 cut(s) 290, 327
AcuI CTGAAG 1 cut(s) 75
AdeI CACNNNGTG 1 cut(s) 80
AfaI GTAC 2 cut(s) 112, 238
AfiI CCNNNNNNNGG 1 cut(s) 61
AgsI TTSAA 3 cut(s) 452, 465, 557
AleI CACNNNNGTG 1 cut(s) 63
AluBI AGCT 1 cut(s) 95
AluI AGCT 1 cut(s) 95
Alw26I GTCTC 1 cut(s) 10
AlwI GGATC 2 cut(s) 290, 327
Ama87I CYCGRG 1 cut(s) 61
AoxI GGCC 1 cut(s) 372
ApeKI GCWGC 2 cut(s) 275, 392
AspLEI GCGC 2 cut(s) 34, 229
AsuC2I CCSGG 2 cut(s) 62, 63
AsuHPI GGTGA 1 cut(s) 92
AvaI CYCGRG 1 cut(s) 61
AxyI CCTNAGG 1 cut(s) 159
BaeI ACNNNNGTAYC 2 cut(s) 228, 261
BbsI GAAGAC 2 cut(s) 153, 564
BbvI GCAGC 2 cut(s) 262, 379
BccI CCATC 1 cut(s) 508
BcgI CGANNNNNNTGC 2 cut(s) 510, 544
BclI TGATCA 1 cut(s) 97
BcnI CCSGG 2 cut(s) 62, 63
BcoDI GTCTC 1 cut(s) 10
BfmI CTRYAG 1 cut(s) 276
BfoI RGCGCY 1 cut(s) 35
BisI GCNGC 3 cut(s) 35, 276, 393
BlsI GCNGC 3 cut(s) 36, 277, 394
Bme1390I CCNGG 2 cut(s) 62, 63
BmeT110I CYCGRG 1 cut(s) 61
BmrFI CCNGG 2 cut(s) 62, 63
BmsI GCATC 1 cut(s) 355
BpiI GAAGAC 2 cut(s) 153, 564
BpuEI CTTGAG 1 cut(s) 5
BpuMI CCSGG 2 cut(s) 62, 63
BsaBI GATNNNNATC 1 cut(s) 353
BsaI GGTCTC 1 cut(s) 10
BsaJI CCNNGG 2 cut(s) 61, 387
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse1I ACTGG 1 cut(s) 549
Bse21I CCTNAGG 1 cut(s) 159
Bse3DI GCAATG 1 cut(s) 375
Bse8I GATNNNNATC 1 cut(s) 353
BseDI CCNNGG 2 cut(s) 61, 387
BseGI GGATG 1 cut(s) 219
BseJI GATNNNNATC 1 cut(s) 353
BseLI CCNNNNNNNGG 1 cut(s) 61
BseMI GCAATG 1 cut(s) 375
BseMII CTCAG 1 cut(s) 173
BseNI ACTGG 1 cut(s) 549
BseXI GCAGC 2 cut(s) 262, 379
Bsh1236I CGCG 1 cut(s) 12
BshFI GGCC 1 cut(s) 374
BsiHKCI CYCGRG 1 cut(s) 61
BsiSI CCGG 1 cut(s) 62
BslFI GGGAC 1 cut(s) 492
BslI CCNNNNNNNGG 1 cut(s) 61
BsmAI GTCTC 1 cut(s) 10
BsmFI GGGAC 1 cut(s) 492
BsmI GAATGC 1 cut(s) 521
BsnI GGCC 1 cut(s) 374
Bso31I GGTCTC 1 cut(s) 10
BsoBI CYCGRG 1 cut(s) 61
Bsp1407I TGTACA 1 cut(s) 236
Bsp143I GATC 3 cut(s) 97, 282, 319
BspACI CCGC 2 cut(s) 10, 35
BspANI GGCC 1 cut(s) 374
BspCNI CTCAG 1 cut(s) 172
BspFNI CGCG 1 cut(s) 12
BspHI TCATGA 1 cut(s) 142
BspMAI CTGCAG 1 cut(s) 280
BspPI GGATC 2 cut(s) 290, 327
BspTNI GGTCTC 1 cut(s) 10
BsrDI GCAATG 1 cut(s) 375
BsrGI TGTACA 1 cut(s) 236
BsrI ACTGG 1 cut(s) 549
BssECI CCNNGG 2 cut(s) 61, 387
BssMI GATC 3 cut(s) 97, 282, 319
BssT1I CCWWGG 1 cut(s) 387
Bst4CI ACNGT 2 cut(s) 241, 289
BstAUI TGTACA 1 cut(s) 236
BstDEI CTNAG 1 cut(s) 159
BstF5I GGATG 1 cut(s) 219
BstFNI CGCG 1 cut(s) 12
BstH2I RGCGCY 1 cut(s) 35
BstHHI GCGC 2 cut(s) 34, 229
BstKTI GATC 3 cut(s) 100, 285, 322
BstMAI GTCTC 1 cut(s) 10
BstMBI GATC 3 cut(s) 97, 282, 319
BstMWI GCNNNNNNNGC 3 cut(s) 92, 272, 392
BstSCI CCNGG 2 cut(s) 60, 61
BstSFI CTRYAG 1 cut(s) 276
BstUI CGCG 1 cut(s) 12
BstV1I GCAGC 2 cut(s) 262, 379
BstV2I GAAGAC 2 cut(s) 153, 564
BstXI CCANNNNNNTGG 1 cut(s) 65
Bsu36I CCTNAGG 1 cut(s) 159
BsuRI GGCC 1 cut(s) 374
BtsCI GGATG 1 cut(s) 219
BtsIMutI CAGTG 2 cut(s) 285, 556
CciI TCATGA 1 cut(s) 142
CfoI GCGC 2 cut(s) 34, 229
Cfr9I CCCGGG 1 cut(s) 61
Csp6I GTAC 2 cut(s) 111, 237
CviAII CATG 4 cut(s) 77, 122, 143, 234
CviJI RGCY 5 cut(s) 86, 95, 374, 392, 533
CviKI_1 RGCY 5 cut(s) 86, 95, 374, 392, 533
CviQI GTAC 2 cut(s) 111, 237
DdeI CTNAG 1 cut(s) 159
DpnI GATC 3 cut(s) 99, 284, 321
DpnII GATC 3 cut(s) 97, 282, 319
DraIII CACNNNGTG 1 cut(s) 80
Eco130I CCWWGG 1 cut(s) 387
Eco31I GGTCTC 1 cut(s) 10
Eco57I CTGAAG 1 cut(s) 75
Eco81I CCTNAGG 1 cut(s) 159
Eco88I CYCGRG 1 cut(s) 61
EcoT14I CCWWGG 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 387
FaeI CATG 4 cut(s) 80, 125, 146, 237
FaiI YATR 7 cut(s) 78, 123, 144, 183, 235, 432, 506
FaqI GGGAC 1 cut(s) 492
FatI CATG 4 cut(s) 76, 121, 142, 233
FauI CCCGC 1 cut(s) 17
FbaI TGATCA 1 cut(s) 97
Fnu4HI GCNGC 3 cut(s) 35, 276, 393
FokI GGATG 1 cut(s) 226
Fsp4HI GCNGC 3 cut(s) 35, 276, 393
GlaI GCGC 2 cut(s) 33, 228
GluI GCNGC 3 cut(s) 35, 276, 393
HaeII RGCGCY 1 cut(s) 35
HaeIII GGCC 1 cut(s) 374
HapII CCGG 1 cut(s) 62
HhaI GCGC 2 cut(s) 34, 229
Hin1II CATG 4 cut(s) 80, 125, 146, 237
Hin6I GCGC 2 cut(s) 32, 227
HinP1I GCGC 2 cut(s) 32, 227
HinfI GANTC 3 cut(s) 71, 137, 452
HpaII CCGG 1 cut(s) 62
HphI GGTGA 1 cut(s) 92
Hpy188I TCNGA 3 cut(s) 319, 348, 364
Hpy188III TCNNGA 4 cut(s) 22, 143, 161, 449
HpyAV CCTTC 4 cut(s) 19, 194, 415, 471
HpyCH4III ACNGT 2 cut(s) 241, 289
HpyCH4IV ACGT 1 cut(s) 427
HpyCH4V TGCA 5 cut(s) 131, 278, 331, 368, 395
HpyF10VI GCNNNNNNNGC 3 cut(s) 92, 272, 392
HpyF3I CTNAG 1 cut(s) 159
HpySE526I ACGT 1 cut(s) 427
Hsp92II CATG 4 cut(s) 80, 125, 146, 237
HspAI GCGC 2 cut(s) 32, 227
Ksp22I TGATCA 1 cut(s) 97
Kzo9I GATC 3 cut(s) 97, 282, 319
LpnPI CCDG 6 cut(s) 33, 75, 146, 246, 264, 562
Lsp1109I GCAGC 2 cut(s) 262, 379
LweI GCATC 1 cut(s) 355
MaeII ACGT 1 cut(s) 427
MaeIII GTNAC 1 cut(s) 289
MalI GATC 3 cut(s) 99, 284, 321
MboI GATC 3 cut(s) 97, 282, 319
MboII GAAGA 3 cut(s) 158, 537, 569
MluCI AATT 1 cut(s) 185
MnlI CCTC 3 cut(s) 65, 168, 293
MslI CAYNNNNRTG 2 cut(s) 63, 126
MspA1I CMGCKG 1 cut(s) 95
MspI CCGG 1 cut(s) 62
MspR9I CCNGG 2 cut(s) 62, 63
Mva1269I GAATGC 1 cut(s) 521
MvnI CGCG 1 cut(s) 12
MwoI GCNNNNNNNGC 3 cut(s) 92, 272, 392
NciI CCSGG 2 cut(s) 62, 63
NdeII GATC 3 cut(s) 97, 282, 319
NlaIII CATG 4 cut(s) 80, 125, 146, 237
NmeAIII GCCGAG 1 cut(s) 509
NmuCI GTSAC 1 cut(s) 289
OliI CACNNNNGTG 1 cut(s) 63
PagI TCATGA 1 cut(s) 142
PctI GAATGC 1 cut(s) 521
PfeI GAWTC 3 cut(s) 71, 137, 452
PkrI GCNGC 3 cut(s) 36, 277, 394
PsiI TTATAA 1 cut(s) 506
PstI CTGCAG 1 cut(s) 280
PvuII CAGCTG 1 cut(s) 95
RsaI GTAC 2 cut(s) 112, 238
RsaNI GTAC 2 cut(s) 111, 237
RseI CAYNNNNRTG 2 cut(s) 63, 126
SatI GCNGC 3 cut(s) 35, 276, 393
Sau3AI GATC 3 cut(s) 97, 282, 319
ScrFI CCNGG 2 cut(s) 62, 63
SetI ASST 5 cut(s) 57, 97, 407, 430, 463
SfaNI GCATC 1 cut(s) 355
SfcI CTRYAG 1 cut(s) 276
SmaI CCCGGG 1 cut(s) 63
SmiMI CAYNNNNRTG 2 cut(s) 63, 126
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
Sse9I AATT 1 cut(s) 185
SsiI CCGC 2 cut(s) 10, 35
StyD4I CCNGG 2 cut(s) 60, 61
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 2 cut(s) 241, 289
TaiI ACGT 1 cut(s) 430
TasI AATT 1 cut(s) 185
TatI WGTACW 2 cut(s) 110, 236
TauI GCSGC 1 cut(s) 37
TfiI GAWTC 3 cut(s) 71, 137, 452
TscAI CASTG 2 cut(s) 292, 556
TseFI GTSAC 1 cut(s) 289
TseI GCWGC 2 cut(s) 275, 392
Tsp45I GTSAC 1 cut(s) 289
TspDTI ATGAA 4 cut(s) 159, 230, 343, 526
TspMI CCCGGG 1 cut(s) 61
TspRI CASTG 2 cut(s) 292, 556
XmaI CCCGGG 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.