MD03G1147100.v1.1

Domain of unknown function

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000157
Physical Location & Seq
Reverse (-)
16171092 .. 16181145
10054 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1147100.v1.1.491

Sequence Viewer

Length: 1797 bp
ATGACGGCAGTAAATGGCGTTCCGGCTGCTGGAAATGTAATCATCGGCATTGCGGCTTCTACGCCGATTCAACCGAGGCATCCTTCTCGTCATCTACTAGAAAATACCAATAGAGAGGCATACTTGAACTTGTGTATGCCCCTCTACAAAGCTGCAATGGAAGGCAATTGGGAAACCGCCAAAGGCATCTTAAATATCAATCCGGCACTCTTAACTACTAGCATAACAAACGGATGGGAAACTGTTCTTCACGTTGCAGCTGGAGCAAAGAACAATATTCAGATTGTGGCGGAACTAGTCAAGATGATGGATGAAGAAGACTTGGCACTTCAAGATATTAAAGGAAATACGGCACTGAGCTTTGCCGCTGCAACTGGAACGGTGGAAATTGCCAAGATTTTGATAGGAAAAAATAAGTTATTGCCGACAATCCCCGGTGGTCAAGGAATGACACCACTCTATATGGCTGCTTTGTTTGGACAGTCTGAAATGGCGTGGTATTTATACCCTCAAACATTTGAAATGCTGGATGAAATGGGCCGCATTGTTCTATTTTTCTGTTCTATCGATAATGGTCTTTATGATTTAGCCATCAAGTTGCTACAAGATGATAGAACATTAGCAATGGCTCGCAAAGGCGACAATGAAACAGCCTTGCATGTCTTAGCTCGAAAGTCTTCAGAATTTGGCGACCAAAGTACTCCAAGAATGTGCAGTAGACTTATCAAAGCATTTAAAGTACTCCCATGCATGAGGGTTTCTTACAAAAGAAACTTGAAGCAAACCCAAGCCTTGAAGCTAGTCGAATGCCTTTGGAAAGAAATGTTGAAACATGACGACGACGAAGTCATGCATTTGATAAGAGAGCCTTCAGAATTAGTATTCGACGCAGCGAAATTAGGAAATTATGAGTTCTTATCTGTGCTTATTGACTCTTATCCGGAATTACTATTGGAATATGATGACAACAATCGGACCATATTCCATATTGCAGTCTTGCATCGTCATGCAAGTATCTTCAATCTAGTGCATGAGACAGGCTCCATCAAAGATATCATAGCAACATTTACTGATGACGAGAATAACAACATTCTACACCTAGCTGCAAAATTGGCACCTCAAGACCAACTCAATCTTGTGTCCGGAGCAGCTCTTCAGATGCAGCGAGAATTAGTATGGTTTGAGGAAGTTGAGAAGATTGTGCAACCTCCCTTTATAGATATGAAAAATACGCAAGGTAAAACACCTCGAGAATTATTCACTAGCGAGCATGAGGATCTATTGCGCAAGGGAGAATCATGGATGAAGGACACTACAAATTCATGCATGCTAGTTTCAACTCTTATCGCCACTGTTGTGTTTTCAGCCGCATTTAGCATTCCGGGAGGTATAGATGATAATAAAGGAACACCGAATTTCATAAAAGAGGAGGCATTTTTAATATTCGCCATATCTGATGGAGTAGCACTCTTTTCCTCCTCAACTGCAATCCTCATGTTCTTGTACATCCTCACGTCGCGGTATGCTGAAAACGATTTTCTCAAATCTTTACCGTTGAAGTTGATGGTCGGACTCACATCACTCTTCGTGTCAATAACATCCATGATGGTAACTTTCAGCACAGCCTTCTATTTATCTTGCCATTATGGATTAAGATGGGTTCCAGATCTTATATTCATATTTGCATTTGTCCCAGTTGCTTTGTTCGCTTTCTTGCAATATCCTCTCTTGTTTGATATGTTCTTTTCGACATATTGTTCAAGTCTTCTGTTTCAGCCAAGGAGGGATATGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

599

Amino Acids

67.06

Weight (kDa)

5.41

Isoelectric Point (pI)

39.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 48 - 137 7.4e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 83 - 170 4.3e-11 Ankyrin repeats (3 copies)
PGG PF13962 433 - 545 6.9e-28 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54070 AT5G35830
fragaria_vesca FvH4_3g25070 FvH4_3g25080 FvH4_3g25081 FvH4_3g25081
malus_domestica MD03G1027500.v1.1 MD03G1146300.v1.1 MD03G1146800.v1.1 MD03G1146900.v1.1 MD03G1147100.v1.1 MD11G1165600.v1.1
prunus_persica Prupe.2G015800_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G016300_v2.0.a1 Prupe.2G016500_v2.0.a1 Prupe.6G131100_v2.0.a1 Prupe.6G131400_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1
pyrus_communis pycom03g10090 pycom03g10110 pycom11g13740 pycom11g13770
rosa_chinensis RchiOBHm_Chr1g0316371 RchiOBHm_Chr1g0316381 RchiOBHm_Chr1g0316401 RchiOBHm_Chr1g0316411 RchiOBHm_Chr1g0316571 RchiOBHm_Chr1g0316601 RchiOBHm_Chr5g0039341 RchiOBHm_Chr5g0041371 RchiOBHm_Chr5g0041401 RchiOBHm_Chr5g0041431 RchiOBHm_Chr5g0041471 RchiOBHm_Chr5g0045281 RchiOBHm_Chr5g0045291 RchiOBHm_Chr5g0045311 RchiOBHm_Chr5g0045371 RchiOBHm_Chr5g0045421 RchiOBHm_Chr5g0045431 RchiOBHm_Chr5g0045501 RchiOBHm_Chr5g0045551 RchiOBHm_Chr5g0045771 RchiOBHm_Chr5g0045811
rosa_laevigata RLG00000034057 RLG00000034058
rosa_multiflora Rmu_co8352751.1_g000001 Rmu_co8397575.1_g000001 Rmu_sc0000415.1_g000006 Rmu_sc0000493.1_g000057 Rmu_sc0000504.1_g000003 Rmu_sc0000594.1_g000019 Rmu_sc0000594.1_g000032 Rmu_sc0000594.1_g000066 Rmu_sc0001047.1_g000003 Rmu_sc0001047.1_g000011 Rmu_sc0001556.1_g000023 Rmu_sc0002584.1_g000003 Rmu_sc0002584.1_g000039 Rmu_sc0002584.1_g000070 Rmu_sc0002667.1_g000015 Rmu_sc0003677.1_g000003 Rmu_sc0003743.1_g000017 Rmu_sc0004520.1_g000004 Rmu_sc0004520.1_g000013 Rmu_sc0004520.1_g000027 Rmu_sc0005539.1_g000014 Rmu_sc0005539.1_g000032 Rmu_sc0006018.1_g000006 Rmu_sc0006737.1_g000020 Rmu_sc0007485.1_g000002 Rmu_sc0008729.1_g000002 Rmu_sc0008729.1_g000003 Rmu_sc0038822.1_g000001 Rmu_ssc0000398.1_g000025
rosa_roxburghii Rroxscaffold_1G00035090 Rroxscaffold_1G00035630 Rroxscaffold_1G00035640 Rroxscaffold_1G00035660 Rroxscaffold_1G00035680 Rroxscaffold_1G00035730 Rroxscaffold_1G00035750 Rroxscaffold_1G00035770 Rroxscaffold_1G00039150 Rroxscaffold_1G00039190 Rroxscaffold_1G00039220 Rroxscaffold_1G00039260 Rroxscaffold_4G00328910 Rroxscaffold_4G00328950 Rroxscaffold_4G00328960 Rroxscaffold_4G00330890
rosa_rugosa Rorug01G0009800 Rorug01G0010000.1 Rorug01G0010200 Rorug01G0012100 Rorug01G0023500 Rorug01G0023600 Rorug01G0023700 Rorug01G0024700 Rorug05G0192000 Rorug05G0192200 Rorug05G0222400 Rorug05G0222500 Rorug05G0222600.1 Rorug05G0222700.1 Rorug05G0222800.1 Rorug05G0222900.1 Rorug05G0223000.1 Rorug05G0223100.1 Rorug05G0223200 Rorug05G0223600 Rorug05G0226800 Rorug05G0226900
rosa_samantha Rh1CG019600 Rh1DG015000 Rh1DG015300 Rh1DG016900 Rh5CG315200 Rh5CG338700 Rh5DG277100 Rh5DG291500 Rh5DG292100 Rh5DG292200 Rh5DG292400 Rh5DG292600 Rh5DG322100 Rh5DG322200 Rh5DG323100 Rh5DG323300 Rh5DG326700 Rh5DG326900 Rh5DG327000
rosa_wichuraiana Rw0G021580 Rw1G001320 Rw1G001420 Rw1G002820 Rw1G002840 Rw1G002870 Rw5G024800 Rw5G026160 Rw5G026170 Rw5G026180 Rw5G026210 Rw5G026230 Rw5G028170 Rw5G028190 Rw5G028210 Rw5G028230 Rw5G028240 Rw5G028260 Rw5G028280 Rw5G028370 Rw5G028380 Rw5G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 845
Acc16I TGCGCA 1 cut(s) 1286
AccB1I GGYRCC 1 cut(s) 1114
AccI GTMKAC 1 cut(s) 718
AccII CGCG 1 cut(s) 1519
AccIII TCCGGA 2 cut(s) 940, 1142
AciI CCGC 7 cut(s) 53, 177, 290, 366, 541, 1368, 1519
AclWI GGATC 1 cut(s) 1284
AcsI RAATTY 3 cut(s) 683, 1318, 1414
AcuI CTGAAG 3 cut(s) 663, 855, 1139
AfaI GTAC 3 cut(s) 700, 741, 1505
AfiI CCNNNNNNNGG 1 cut(s) 29
AhlI ACTAGT 1 cut(s) 295
AjiI CACGTC 1 cut(s) 1515
AleI CACNNNNGTG 1 cut(s) 1355
AluBI AGCT 7 cut(s) 152, 260, 360, 668, 799, 1103, 1151
AluI AGCT 7 cut(s) 152, 260, 360, 668, 799, 1103, 1151
Alw26I GTCTC 1 cut(s) 1028
AlwI GGATC 1 cut(s) 1284
Ama87I CYCGRG 1 cut(s) 1248
Aor13HI TCCGGA 2 cut(s) 940, 1142
AoxI GGCC 1 cut(s) 538
ApeKI GCWGC 9 cut(s) 26, 152, 257, 368, 467, 890, 1103, 1148, 1162
ApoI RAATTY 3 cut(s) 683, 1318, 1414
Asp700I GAANNNNTTC 2 cut(s) 243, 676
AspLEI GCGC 1 cut(s) 1287
AspS9I GGNCC 2 cut(s) 538, 975
AsuC2I CCSGG 2 cut(s) 435, 1383
AvaI CYCGRG 1 cut(s) 1248
AvaII GGWCC 1 cut(s) 975
BanI GGYRCC 1 cut(s) 1114
BbsI GAAGAC 3 cut(s) 324, 669, 1757
BbvI GCAGC 9 cut(s) 13, 139, 269, 355, 454, 902, 1090, 1160, 1174
BccI CCATC 8 cut(s) 228, 301, 599, 1052, 1451, 1558, 1600, 1650
BceAI ACGGC 2 cut(s) 21, 366
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BcnI CCSGG 2 cut(s) 435, 1383
BcoDI GTCTC 1 cut(s) 1028
BcuI ACTAGT 1 cut(s) 295
BfaI CTAG 8 cut(s) 98, 219, 296, 800, 1025, 1100, 1263, 1331
BglII AGATCT 1 cut(s) 1666
BmcAI AGTACT 2 cut(s) 700, 741
Bme1390I CCNGG 2 cut(s) 435, 1383
Bme18I GGWCC 1 cut(s) 975
BmeT110I CYCGRG 1 cut(s) 1248
BmgBI CACGTC 1 cut(s) 1515
BmgT120I GGNCC 2 cut(s) 538, 975
BmiI GGNNCC 3 cut(s) 1042, 1116, 1662
BmrFI CCNGG 2 cut(s) 435, 1383
BmrI ACTGGG 1 cut(s) 1688
BmsI GCATC 4 cut(s) 88, 195, 1009, 1149
BmuI ACTGGG 1 cut(s) 1688
BpiI GAAGAC 3 cut(s) 324, 669, 1757
BplI GAGNNNNNCTC 4 cut(s) 1025, 1057, 1452, 1484
BpmI CTGGAG 1 cut(s) 282
BpuEI CTTGAG 1 cut(s) 1104
BpuMI CCSGG 2 cut(s) 435, 1383
Bsa29I ATCGAT 1 cut(s) 567
BsaJI CCNNGG 3 cut(s) 74, 433, 1778
BsaWI WCCGGW 2 cut(s) 940, 1142
Bsc4I CCNNNNNNNGG 1 cut(s) 29
Bse1I ACTGG 2 cut(s) 379, 1694
Bse3DI GCAATG 3 cut(s) 48, 162, 630
BseAI TCCGGA 2 cut(s) 940, 1142
BseCI ATCGAT 1 cut(s) 567
BseDI CCNNGG 3 cut(s) 74, 433, 1778
BseGI GGATG 7 cut(s) 79, 239, 316, 535, 1308, 1506, 1598
BseLI CCNNNNNNNGG 1 cut(s) 29
BseMI GCAATG 3 cut(s) 48, 162, 630
BseMII CTCAG 1 cut(s) 347
BseNI ACTGG 2 cut(s) 379, 1694
BseRI GAGGAG 2 cut(s) 1442, 1468
BseXI GCAGC 9 cut(s) 13, 139, 269, 355, 454, 902, 1090, 1160, 1174
BsgI GTGCAG 1 cut(s) 733
Bsh1236I CGCG 1 cut(s) 1519
BshFI GGCC 1 cut(s) 540
BshNI GGYRCC 1 cut(s) 1114
BshVI ATCGAT 1 cut(s) 567
BsiHKCI CYCGRG 1 cut(s) 1248
BsiSI CCGG 6 cut(s) 23, 203, 435, 941, 1143, 1382
BslFI GGGAC 1 cut(s) 1676
BslI CCNNNNNNNGG 1 cut(s) 29
BsmAI GTCTC 1 cut(s) 1028
BsmFI GGGAC 1 cut(s) 1676
BsmI GAATGC 2 cut(s) 812, 1377
BsnI GGCC 1 cut(s) 540
BsoBI CYCGRG 1 cut(s) 1248
Bsp13I TCCGGA 2 cut(s) 940, 1142
Bsp1407I TGTACA 1 cut(s) 1503
Bsp143I GATC 2 cut(s) 1276, 1666
BspACI CCGC 7 cut(s) 53, 177, 290, 366, 541, 1368, 1519
BspANI GGCC 1 cut(s) 540
BspCNI CTCAG 1 cut(s) 348
BspDI ATCGAT 1 cut(s) 567
BspEI TCCGGA 2 cut(s) 940, 1142
BspFNI CGCG 1 cut(s) 1519
BspLI GGNNCC 3 cut(s) 1042, 1116, 1662
BspPI GGATC 1 cut(s) 1284
BspQI GCTCTTC 1 cut(s) 1158
BspT107I GGYRCC 1 cut(s) 1114
BsrDI GCAATG 3 cut(s) 48, 162, 630
BsrGI TGTACA 1 cut(s) 1503
BsrI ACTGG 2 cut(s) 379, 1694
BssECI CCNNGG 3 cut(s) 74, 433, 1778
BssMI GATC 2 cut(s) 1276, 1666
BssT1I CCWWGG 1 cut(s) 1778
Bst4CI ACNGT 5 cut(s) 244, 382, 483, 1354, 1554
Bst6I CTCTTC 2 cut(s) 1158, 1589
BstAUI TGTACA 1 cut(s) 1503
BstC8I GCNNGC 3 cut(s) 631, 1268, 1328
BstDEI CTNAG 2 cut(s) 356, 664
BstF5I GGATG 7 cut(s) 79, 239, 316, 535, 1308, 1506, 1598
BstFNI CGCG 1 cut(s) 1519
BstHHI GCGC 1 cut(s) 1287
BstKTI GATC 2 cut(s) 1279, 1669
BstMAI GTCTC 1 cut(s) 1028
BstMBI GATC 2 cut(s) 1276, 1666
BstMWI GCNNNNNNNGC 3 cut(s) 263, 1112, 1706
BstNSI RCATGY 2 cut(s) 662, 1330
BstSCI CCNGG 2 cut(s) 433, 1381
BstUI CGCG 1 cut(s) 1519
BstV1I GCAGC 9 cut(s) 13, 139, 269, 355, 454, 902, 1090, 1160, 1174
BstV2I GAAGAC 3 cut(s) 324, 669, 1757
BstX2I RGATCY 2 cut(s) 1276, 1666
BstYI RGATCY 2 cut(s) 1276, 1666
Bsu15I ATCGAT 1 cut(s) 567
BsuRI GGCC 1 cut(s) 540
BsuTUI ATCGAT 1 cut(s) 567
BtrI CACGTC 1 cut(s) 1515
BtsCI GGATG 7 cut(s) 79, 239, 316, 535, 1308, 1506, 1598
BtsIMutI CAGTG 2 cut(s) 353, 1350
Cac8I GCNNGC 3 cut(s) 631, 1268, 1328
CfoI GCGC 1 cut(s) 1287
Cfr13I GGNCC 2 cut(s) 538, 975
ClaI ATCGAT 1 cut(s) 567
CseI GACGC 1 cut(s) 896
Csp6I GTAC 3 cut(s) 699, 740, 1504
CviQI GTAC 3 cut(s) 699, 740, 1504
DdeI CTNAG 2 cut(s) 356, 664
DpnI GATC 2 cut(s) 1278, 1668
DpnII GATC 2 cut(s) 1276, 1666
DraI TTTAAA 1 cut(s) 736
DrdI GACNNNNNNGTC 1 cut(s) 845
DseDI GACNNNNNNGTC 1 cut(s) 845
Eam1104I CTCTTC 2 cut(s) 1158, 1589
EarI CTCTTC 2 cut(s) 1158, 1589
EciI GGCGGA 1 cut(s) 305
Eco130I CCWWGG 1 cut(s) 1778
Eco32I GATATC 1 cut(s) 1054
Eco47I GGWCC 1 cut(s) 975
Eco57I CTGAAG 3 cut(s) 663, 855, 1139
Eco88I CYCGRG 1 cut(s) 1248
EcoRV GATATC 1 cut(s) 1054
EcoT14I CCWWGG 1 cut(s) 1778
EcoT22I ATGCAT 3 cut(s) 752, 855, 1328
ErhI CCWWGG 1 cut(s) 1778
FalI AAGNNNNNCTT 2 cut(s) 853, 885
FaqI GGGAC 1 cut(s) 1676
FblI GTMKAC 1 cut(s) 718
FokI GGATG 7 cut(s) 66, 246, 323, 542, 1315, 1493, 1585
FspBI CTAG 8 cut(s) 98, 219, 296, 800, 1025, 1100, 1263, 1331
FspI TGCGCA 1 cut(s) 1286
GlaI GCGC 1 cut(s) 1286
GsuI CTGGAG 1 cut(s) 282
HaeIII GGCC 1 cut(s) 540
HapII CCGG 6 cut(s) 23, 203, 435, 941, 1143, 1382
HgaI GACGC 1 cut(s) 896
HhaI GCGC 1 cut(s) 1287
Hin6I GCGC 1 cut(s) 1285
HinP1I GCGC 1 cut(s) 1285
HinfI GANTC 4 cut(s) 67, 932, 1295, 1572
HpaII CCGG 6 cut(s) 23, 203, 435, 941, 1143, 1382
Hpy166II GTNNAC 1 cut(s) 719
Hpy188I TCNGA 8 cut(s) 282, 487, 682, 874, 975, 1158, 1456, 1571
Hpy188III TCNNGA 7 cut(s) 301, 332, 941, 1121, 1143, 1250, 1664
Hpy8I GTNNAC 1 cut(s) 719
Hpy99I CGWCG 4 cut(s) 842, 845, 890, 1519
HpyAV CCTTC 5 cut(s) 93, 155, 879, 1300, 1636
HpyCH4III ACNGT 5 cut(s) 244, 382, 483, 1354, 1554
HpyCH4IV ACGT 2 cut(s) 252, 1514
HpyF10VI GCNNNNNNNGC 3 cut(s) 263, 1112, 1706
HpyF3I CTNAG 2 cut(s) 356, 664
HpySE526I ACGT 2 cut(s) 252, 1514
HspAI GCGC 1 cut(s) 1285
Kpn2I TCCGGA 2 cut(s) 940, 1142
Kzo9I GATC 2 cut(s) 1276, 1666
LguI GCTCTTC 1 cut(s) 1158
LmnI GCTCC 3 cut(s) 263, 1046, 1145
Lsp1109I GCAGC 9 cut(s) 13, 139, 269, 355, 454, 902, 1090, 1160, 1174
LweI GCATC 4 cut(s) 88, 195, 1009, 1149
MaeI CTAG 8 cut(s) 98, 219, 296, 800, 1025, 1100, 1263, 1331
MaeII ACGT 2 cut(s) 252, 1514
MaeIII GTNAC 1 cut(s) 1609
MalI GATC 2 cut(s) 1278, 1668
MboI GATC 2 cut(s) 1276, 1666
MboII GAAGA 9 cut(s) 239, 326, 329, 669, 1009, 1145, 1207, 1576, 1757
MfeI CAATTG 1 cut(s) 166
MflI RGATCY 2 cut(s) 1276, 1666
MlyI GAGTC 2 cut(s) 926, 1566
MmeI TCCRAC 1 cut(s) 1549
Mph1103I ATGCAT 3 cut(s) 752, 855, 1328
MroI TCCGGA 2 cut(s) 940, 1142
MroXI GAANNNNTTC 2 cut(s) 243, 676
MseI TTAA 7 cut(s) 191, 212, 339, 735, 1439, 1652, 1795
MslI CAYNNNNRTG 2 cut(s) 1005, 1355
MspA1I CMGCKG 2 cut(s) 260, 368
MspI CCGG 6 cut(s) 23, 203, 435, 941, 1143, 1382
MspR9I CCNGG 2 cut(s) 435, 1383
MunI CAATTG 1 cut(s) 166
Mva1269I GAATGC 2 cut(s) 812, 1377
MvnI CGCG 1 cut(s) 1519
MwoI GCNNNNNNNGC 3 cut(s) 263, 1112, 1706
NciI CCSGG 2 cut(s) 435, 1383
NdeII GATC 2 cut(s) 1276, 1666
NlaIV GGNNCC 3 cut(s) 1042, 1116, 1662
NsbI TGCGCA 1 cut(s) 1286
NsiI ATGCAT 3 cut(s) 752, 855, 1328
NspI RCATGY 2 cut(s) 662, 1330
OliI CACNNNNGTG 1 cut(s) 1355
PaeI GCATGC 1 cut(s) 1330
PaeR7I CTCGAG 1 cut(s) 1248
PciSI GCTCTTC 1 cut(s) 1158
PctI GAATGC 2 cut(s) 812, 1377
PdmI GAANNNNTTC 2 cut(s) 243, 676
PfeI GAWTC 2 cut(s) 67, 1295
PflFI GACNNNGTC 1 cut(s) 845
PfoI TCCNGGA 1 cut(s) 1381
PleI GAGTC 2 cut(s) 926, 1566
PpsI GAGTC 2 cut(s) 926, 1566
PspN4I GGNNCC 3 cut(s) 1042, 1116, 1662
PspPI GGNCC 2 cut(s) 538, 975
PsrI GAACNNNNNNTAC 1 cut(s) 35
PsuI RGATCY 2 cut(s) 1276, 1666
PsyI GACNNNGTC 1 cut(s) 845
PvuII CAGCTG 1 cut(s) 260
RsaI GTAC 3 cut(s) 700, 741, 1505
RsaNI GTAC 3 cut(s) 699, 740, 1504
RseI CAYNNNNRTG 2 cut(s) 1005, 1355
SapI GCTCTTC 1 cut(s) 1158
SaqAI TTAA 7 cut(s) 191, 212, 339, 735, 1439, 1652, 1795
Sau3AI GATC 2 cut(s) 1276, 1666
Sau96I GGNCC 2 cut(s) 538, 975
ScaI AGTACT 2 cut(s) 700, 741
SchI GAGTC 2 cut(s) 926, 1566
ScrFI CCNGG 2 cut(s) 435, 1383
SfaNI GCATC 4 cut(s) 88, 195, 1009, 1149
Sfr274I CTCGAG 1 cut(s) 1248
SinI GGWCC 1 cut(s) 975
SlaI CTCGAG 1 cut(s) 1248
SmiMI CAYNNNNRTG 2 cut(s) 1005, 1355
SmlI CTYRAG 2 cut(s) 1119, 1248
SmoI CTYRAG 2 cut(s) 1119, 1248
SpeI ACTAGT 1 cut(s) 295
SphI GCATGC 1 cut(s) 1330
SsiI CCGC 7 cut(s) 53, 177, 290, 366, 541, 1368, 1519
SspI AATATT 2 cut(s) 277, 1443
SspMI CTAG 8 cut(s) 98, 219, 296, 800, 1025, 1100, 1263, 1331
StyD4I CCNGG 2 cut(s) 433, 1381
StyI CCWWGG 1 cut(s) 1778
TaaI ACNGT 5 cut(s) 244, 382, 483, 1354, 1554
TaiI ACGT 2 cut(s) 255, 1517
TaqI TCGA 6 cut(s) 567, 670, 804, 885, 1249, 1748
TatI WGTACW 3 cut(s) 698, 739, 1503
TauI GCSGC 4 cut(s) 56, 368, 543, 1370
TfiI GAWTC 2 cut(s) 67, 1295
Tru1I TTAA 7 cut(s) 191, 212, 339, 735, 1439, 1652, 1795
Tru9I TTAA 7 cut(s) 191, 212, 339, 735, 1439, 1652, 1795
TscAI CASTG 2 cut(s) 360, 1357
TseI GCWGC 9 cut(s) 26, 152, 257, 368, 467, 890, 1103, 1148, 1162
TspDTI ATGAA 8 cut(s) 327, 546, 660, 1238, 1311, 1319, 1408, 1666
TspGWI ACGGA 1 cut(s) 246
TspRI CASTG 2 cut(s) 360, 1357
Tth111I GACNNNGTC 1 cut(s) 845
VpaK11BI GGWCC 1 cut(s) 975
XapI RAATTY 3 cut(s) 683, 1318, 1414
XceI RCATGY 2 cut(s) 662, 1330
XhoI CTCGAG 1 cut(s) 1248
XmiI GTMKAC 1 cut(s) 718
XmnI GAANNNNTTC 2 cut(s) 243, 676
XspI CTAG 8 cut(s) 98, 219, 296, 800, 1025, 1100, 1263, 1331
ZrmI AGTACT 2 cut(s) 700, 741
Zsp2I ATGCAT 3 cut(s) 752, 855, 1328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.