Rroxscaffold_1G00039150

Ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000157
Physical Location & Seq
Forward (+)
56700768 .. 56703913
3146 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00039150.1

Sequence Viewer

Length: 1785 bp
ATGTCTATCCCACAAGCACAACATGTATCACCTTCCGGGATTCAATTAACCACAGCTAGCGGCGATTCAGCAACTGGGATAGGCAACCAATCAACAGACATCGCCATTGACATTTCAGCTGATTCGAATCAACCTAACAGACCTTTGTCTTATTTACTAGAACATACAATGAGAGAAGAATACCTTAAGACATGTGTTGCCCTCTATAAGTATACACTAAAAGGCGAATGGGAAGCCGCTGAACTAATCTTGAAAAAGGATTGGACCAGACTTCCACGAGCTAGCATATCTAGAAGATATGAAACAGTCCTTCATGTTGCAGCAAGAACAAGCCATATTCACTTTGTGGAGAAACTGGTCAGCATGATGGAAGTAGAAGATTTGGCACTGCAGGACGTAAATGGAAACACTGCCTTGTGCATTGCCGCTGCGGCTGGATCTCTAGACATTGTCCAAATTTTGATACAAAAGAACAAGTCTTTGCTAACAATTAGGGGCAGTAAAGATATGGCACCAGCCTATATGGCTGCTATGTCAGGACAGTCCGAAATGGCGTGTTACCTGTACTACGAAAATTTGGCCTTTCGGTTGCTAAATGCTGATAAAGCATTAGCGAAGGCACGTACTAGTTCACAAAAAAATAAAACGGCCTTGCATATCCTGGCTCGAAGACCTTCAGCATTTGGTGGCAGCCAAAGTCCACCAATGTGGCCTAGACTCATTAACTCATGTTTGAAGTTCGCATGCAAAAGAAAGTTGAGAGAAGCTAATGGAGCCCTTCAACTAGTCCACCGCCTCTGGGAAGAAATCTTGAGAGATGAGTATGATGACATGATGAGGCTGATCAAATATCCTTCAAATTTATTCTTTGACGCAGCGGAATTAGGGAATTATGAGTTTCTAGCAGCGCTTATGAGCTCTTATCCTGATTTAGTATGGGAAACTGATGAAAAAAATCGAACCATAATCCACATCGCAGTTCTGCATCGTCACGCAAGTATCTTCAATCTAGTGCATGAAATAGGCTCAATCAAAGATGTCATCGTGACATACAAAGATGTTCAGGGTAACAACATTGTGCATATGGCTGCAAAATTAGCACCTCAAAATCAATTGAATCTCGTGTCAGGCGTAGCTCTTCAAATGCATCGAGAGTTGGTATGGTTTGAGGAAGTCAAAAAGATCGTCCAACCTCAATACATCGAGATGGAAAACGACAAAGGAAAAACACCTCAAGAATTATTTACGGAAGAGCATACGGTGTTAATGCGTCAAGGAGAAAAATGGATGAAGGACACTGCAACTTCATGCATGCTCGTTGCGACTATTATCGCAACCGTTGTGTTCTCAGCTGCATTTAGCATACCGGGTGGCACAGATGATTATACTGGAAAACCAAACCTTTTGAAACAGAAAGCCTTTCTAGACTTTACCATAGCTGACGAAGTAGCACTCTTTTCCTCTTCAACTGCAATGTTGATGTTCTTATTCATCCTTACCTCGCGGTATGCAGAAAATGATTTCCGAAAGTCCTTACCCTTGAAGTTGATGGCAGGACTCACTAGCCTGTTCATATCTATAGCATCGATGATGATGGCTTTCAGCACTGCCTTCTATCTATCTTGTCAATACGAATCAAAATGGGTTTCAAATTTTACATTTTTATTTGCATTTCTCCCAATTGCTTTGTTTGCGCTTATGAAATTTCCTCTCGTGTCTGACATGTCCTCTTCTATATTTTGCTCAAGTCTACTATTTCAGCCGTGGAAACACATGCTCTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

594

Amino Acids

66.93

Weight (kDa)

7.23

Isoelectric Point (pI)

43.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 99 - 158 1.7e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 104 - 189 7.8e-11 Ankyrin repeats (3 copies)
PGG PF13962 427 - 540 4.7e-26 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54070 AT5G35830
fragaria_vesca FvH4_3g25070 FvH4_3g25080 FvH4_3g25081 FvH4_3g25081
malus_domestica MD03G1027500.v1.1 MD03G1146300.v1.1 MD03G1146800.v1.1 MD03G1146900.v1.1 MD03G1147100.v1.1 MD11G1165600.v1.1
prunus_persica Prupe.2G015800_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G016300_v2.0.a1 Prupe.2G016500_v2.0.a1 Prupe.6G131100_v2.0.a1 Prupe.6G131400_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1
pyrus_communis pycom03g10090 pycom03g10110 pycom11g13740 pycom11g13770
rosa_chinensis RchiOBHm_Chr1g0316371 RchiOBHm_Chr1g0316381 RchiOBHm_Chr1g0316401 RchiOBHm_Chr1g0316411 RchiOBHm_Chr1g0316571 RchiOBHm_Chr1g0316601 RchiOBHm_Chr5g0039341 RchiOBHm_Chr5g0041371 RchiOBHm_Chr5g0041401 RchiOBHm_Chr5g0041431 RchiOBHm_Chr5g0041471 RchiOBHm_Chr5g0045281 RchiOBHm_Chr5g0045291 RchiOBHm_Chr5g0045311 RchiOBHm_Chr5g0045371 RchiOBHm_Chr5g0045421 RchiOBHm_Chr5g0045431 RchiOBHm_Chr5g0045501 RchiOBHm_Chr5g0045551 RchiOBHm_Chr5g0045771 RchiOBHm_Chr5g0045811
rosa_laevigata RLG00000034057 RLG00000034058
rosa_multiflora Rmu_co8352751.1_g000001 Rmu_co8397575.1_g000001 Rmu_sc0000415.1_g000006 Rmu_sc0000493.1_g000057 Rmu_sc0000504.1_g000003 Rmu_sc0000594.1_g000019 Rmu_sc0000594.1_g000032 Rmu_sc0000594.1_g000066 Rmu_sc0001047.1_g000003 Rmu_sc0001047.1_g000011 Rmu_sc0001556.1_g000023 Rmu_sc0002584.1_g000003 Rmu_sc0002584.1_g000039 Rmu_sc0002584.1_g000070 Rmu_sc0002667.1_g000015 Rmu_sc0003677.1_g000003 Rmu_sc0003743.1_g000017 Rmu_sc0004520.1_g000004 Rmu_sc0004520.1_g000013 Rmu_sc0004520.1_g000027 Rmu_sc0005539.1_g000014 Rmu_sc0005539.1_g000032 Rmu_sc0006018.1_g000006 Rmu_sc0006737.1_g000020 Rmu_sc0007485.1_g000002 Rmu_sc0008729.1_g000002 Rmu_sc0008729.1_g000003 Rmu_sc0038822.1_g000001 Rmu_ssc0000398.1_g000025
rosa_roxburghii Rroxscaffold_1G00035090 Rroxscaffold_1G00035630 Rroxscaffold_1G00035640 Rroxscaffold_1G00035660 Rroxscaffold_1G00035680 Rroxscaffold_1G00035730 Rroxscaffold_1G00035750 Rroxscaffold_1G00035770 Rroxscaffold_1G00039150 Rroxscaffold_1G00039190 Rroxscaffold_1G00039220 Rroxscaffold_1G00039260 Rroxscaffold_4G00328910 Rroxscaffold_4G00328950 Rroxscaffold_4G00328960 Rroxscaffold_4G00330890
rosa_rugosa Rorug01G0009800 Rorug01G0010000.1 Rorug01G0010200 Rorug01G0012100 Rorug01G0023500 Rorug01G0023600 Rorug01G0023700 Rorug01G0024700 Rorug05G0192000 Rorug05G0192200 Rorug05G0222400 Rorug05G0222500 Rorug05G0222600.1 Rorug05G0222700.1 Rorug05G0222800.1 Rorug05G0222900.1 Rorug05G0223000.1 Rorug05G0223100.1 Rorug05G0223200 Rorug05G0223600 Rorug05G0226800 Rorug05G0226900
rosa_samantha Rh1CG019600 Rh1DG015000 Rh1DG015300 Rh1DG016900 Rh5CG315200 Rh5CG338700 Rh5DG277100 Rh5DG291500 Rh5DG292100 Rh5DG292200 Rh5DG292400 Rh5DG292600 Rh5DG322100 Rh5DG322200 Rh5DG323100 Rh5DG323300 Rh5DG326700 Rh5DG326900 Rh5DG327000
rosa_wichuraiana Rw0G021580 Rw1G001320 Rw1G001420 Rw1G002820 Rw1G002840 Rw1G002870 Rw5G024800 Rw5G026160 Rw5G026170 Rw5G026180 Rw5G026210 Rw5G026230 Rw5G028170 Rw5G028190 Rw5G028210 Rw5G028230 Rw5G028240 Rw5G028260 Rw5G028280 Rw5G028370 Rw5G028380 Rw5G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 511
AccI GTMKAC 2 cut(s) 212, 1750
AccII CGCG 1 cut(s) 1504
AciI CCGC 7 cut(s) 60, 237, 426, 431, 793, 878, 1504
AclWI GGATC 1 cut(s) 445
AcsI RAATTY 5 cut(s) 456, 574, 859, 1651, 1703
AcuI CTGAAG 1 cut(s) 660
AdeI CACNNNGTG 1 cut(s) 346
AfaI GTAC 2 cut(s) 566, 625
AfeI AGCGCT 1 cut(s) 909
AfiI CCNNNNNNNGG 1 cut(s) 799
AflII CTTAAG 1 cut(s) 185
AflIII ACRYGT 3 cut(s) 22, 191, 1722
AhlI ACTAGT 2 cut(s) 626, 784
AjnI CCWGG 1 cut(s) 660
AleI CACNNNNGTG 1 cut(s) 706
AluBI AGCT 8 cut(s) 56, 119, 281, 767, 918, 1136, 1352, 1439
AluI AGCT 8 cut(s) 56, 119, 281, 767, 918, 1136, 1352, 1439
Alw21I GWGCWC 1 cut(s) 920
AlwI GGATC 1 cut(s) 445
AlwNI CAGNNNCTG 1 cut(s) 74
Aor51HI AGCGCT 1 cut(s) 909
AoxI GGCC 3 cut(s) 579, 648, 710
ApeKI GCWGC 8 cut(s) 320, 428, 527, 690, 875, 905, 1088, 1352
ApoI RAATTY 5 cut(s) 456, 574, 859, 1651, 1703
ArsI GACNNNNNNTTYG 2 cut(s) 1170, 1202
Asp700I GAANNNNTTC 1 cut(s) 673
AspLEI GCGC 2 cut(s) 910, 1696
AspS9I GGNCC 1 cut(s) 264
AsuC2I CCSGG 2 cut(s) 37, 1368
AsuHPI GGTGA 1 cut(s) 21
AsuII TTCGAA 1 cut(s) 125
AsuNHI GCTAGC 2 cut(s) 56, 281
AvaII GGWCC 1 cut(s) 264
BaeI ACNNNNGTAYC 2 cut(s) 982, 1015
BanI GGYRCC 1 cut(s) 511
BanII GRGCYC 2 cut(s) 778, 920
BauI CACGAG 3 cut(s) 276, 1121, 1712
BbsI GAAGAC 1 cut(s) 676
Bbv12I GWGCWC 1 cut(s) 920
BbvI GCAGC 8 cut(s) 332, 415, 514, 702, 887, 917, 1075, 1339
BccI CCATC 4 cut(s) 361, 1201, 1543, 1588
BceAI ACGGC 2 cut(s) 663, 1747
BciT130I CCWGG 1 cut(s) 662
BclI TGATCA 1 cut(s) 843
BcnI CCSGG 2 cut(s) 37, 1368
BcuI ACTAGT 2 cut(s) 626, 784
BfmI CTRYAG 2 cut(s) 389, 1578
BfoI RGCGCY 1 cut(s) 911
BfrI CTTAAG 1 cut(s) 185
BglI GCCNNNNNGGC 2 cut(s) 431, 524
Bme1390I CCNGG 3 cut(s) 37, 662, 1368
Bme18I GGWCC 1 cut(s) 264
BmgT120I GGNCC 1 cut(s) 264
BmiI GGNNCC 2 cut(s) 513, 775
BmrFI CCNGG 3 cut(s) 37, 662, 1368
BmrI ACTGGG 1 cut(s) 84
BmsI GCATC 3 cut(s) 994, 1156, 1592
BmtI GCTAGC 2 cut(s) 60, 285
BmuI ACTGGG 1 cut(s) 84
BoxI GACNNNNGTC 1 cut(s) 145
BpiI GAAGAC 1 cut(s) 676
Bpu14I TTCGAA 1 cut(s) 125
BpuEI CTTGAG 3 cut(s) 832, 1218, 1729
BpuMI CCSGG 2 cut(s) 37, 1368
Bsa29I ATCGAT 1 cut(s) 1586
BsaAI YACGTR 1 cut(s) 623
BsaBI GATNNNNATC 1 cut(s) 126
BsaJI CCNNGG 1 cut(s) 1763
Bsc4I CCNNNNNNNGG 1 cut(s) 799
Bse1I ACTGG 3 cut(s) 79, 360, 1393
Bse3DI GCAATG 2 cut(s) 420, 1479
Bse8I GATNNNNATC 1 cut(s) 126
BseBI CCWGG 1 cut(s) 662
BseCI ATCGAT 1 cut(s) 1586
BseDI CCNNGG 1 cut(s) 1763
BseGI GGATG 2 cut(s) 1293, 1491
BseJI GATNNNNATC 1 cut(s) 126
BseLI CCNNNNNNNGG 1 cut(s) 799
BseMI GCAATG 2 cut(s) 420, 1479
BseMII CTCAG 1 cut(s) 1362
BseNI ACTGG 3 cut(s) 79, 360, 1393
BseXI GCAGC 8 cut(s) 332, 415, 514, 702, 887, 917, 1075, 1339
Bsh1236I CGCG 1 cut(s) 1504
BshFI GGCC 3 cut(s) 581, 650, 712
BshNI GGYRCC 1 cut(s) 511
BshVI ATCGAT 1 cut(s) 1586
BsiHKAI GWGCWC 1 cut(s) 920
BsiSI CCGG 2 cut(s) 36, 1367
BslI CCNNNNNNNGG 1 cut(s) 799
BsnI GGCC 3 cut(s) 581, 650, 712
Bsp119I TTCGAA 1 cut(s) 125
Bsp1286I GDGCHC 2 cut(s) 778, 920
Bsp143I GATC 3 cut(s) 437, 843, 1182
BspACI CCGC 7 cut(s) 60, 237, 426, 431, 793, 878, 1504
BspANI GGCC 3 cut(s) 581, 650, 712
BspCNI CTCAG 1 cut(s) 1361
BspDI ATCGAT 1 cut(s) 1586
BspFNI CGCG 1 cut(s) 1504
BspLI GGNNCC 2 cut(s) 513, 775
BspMAI CTGCAG 1 cut(s) 393
BspOI GCTAGC 2 cut(s) 60, 285
BspPI GGATC 1 cut(s) 445
BspQI GCTCTTC 2 cut(s) 1143, 1245
BspT104I TTCGAA 1 cut(s) 125
BspT107I GGYRCC 1 cut(s) 511
BspTI CTTAAG 1 cut(s) 185
BsrDI GCAATG 2 cut(s) 420, 1479
BsrI ACTGG 3 cut(s) 79, 360, 1393
BssECI CCNNGG 1 cut(s) 1763
BssMI GATC 3 cut(s) 437, 843, 1182
BssNAI GTATAC 1 cut(s) 213
BssSI CACGAG 3 cut(s) 276, 1121, 1712
Bst1107I GTATAC 1 cut(s) 213
Bst2BI CACGAG 3 cut(s) 276, 1121, 1712
Bst2UI CCWGG 1 cut(s) 662
Bst4CI ACNGT 4 cut(s) 307, 543, 1261, 1339
Bst6I CTCTTC 4 cut(s) 1143, 1245, 1468, 1735
BstAFI CTTAAG 1 cut(s) 185
BstBAI YACGTR 1 cut(s) 623
BstBI TTCGAA 1 cut(s) 125
BstC8I GCNNGC 4 cut(s) 58, 283, 745, 1313
BstDEI CTNAG 1 cut(s) 1348
BstDSI CCRYGG 1 cut(s) 1763
BstF5I GGATG 2 cut(s) 1293, 1491
BstFNI CGCG 1 cut(s) 1504
BstH2I RGCGCY 1 cut(s) 911
BstHHI GCGC 2 cut(s) 910, 1696
BstKTI GATC 3 cut(s) 440, 846, 1185
BstMBI GATC 3 cut(s) 437, 843, 1182
BstMWI GCNNNNNNNGC 6 cut(s) 431, 524, 605, 773, 1097, 1691
BstNI CCWGG 1 cut(s) 662
BstNSI RCATGY 6 cut(s) 26, 195, 747, 1315, 1726, 1777
BstPAI GACNNNNGTC 1 cut(s) 145
BstSCI CCNGG 3 cut(s) 35, 660, 1366
BstSFI CTRYAG 2 cut(s) 389, 1578
BstUI CGCG 1 cut(s) 1504
BstV1I GCAGC 8 cut(s) 332, 415, 514, 702, 887, 917, 1075, 1339
BstV2I GAAGAC 1 cut(s) 676
BstX2I RGATCY 1 cut(s) 437
BstXI CCANNNNNNTGG 1 cut(s) 708
BstYI RGATCY 1 cut(s) 437
BstZ17I GTATAC 1 cut(s) 213
Bsu15I ATCGAT 1 cut(s) 1586
BsuRI GGCC 3 cut(s) 581, 650, 712
BsuTUI ATCGAT 1 cut(s) 1586
BtgI CCRYGG 1 cut(s) 1763
BtgZI GCGATG 2 cut(s) 85, 958
BtsCI GGATG 2 cut(s) 1293, 1491
BtsI GCAGTG 4 cut(s) 386, 408, 1296, 1605
BtsIMutI CAGTG 4 cut(s) 386, 408, 1296, 1605
Cac8I GCNNGC 4 cut(s) 58, 283, 745, 1313
CaiI CAGNNNCTG 1 cut(s) 74
CfoI GCGC 2 cut(s) 910, 1696
Cfr13I GGNCC 1 cut(s) 264
ClaI ATCGAT 1 cut(s) 1586
CseI GACGC 2 cut(s) 881, 1259
Csp6I GTAC 2 cut(s) 565, 624
CviQI GTAC 2 cut(s) 565, 624
DdeI CTNAG 1 cut(s) 1348
DpnI GATC 3 cut(s) 439, 845, 1184
DpnII GATC 3 cut(s) 437, 843, 1182
DraIII CACNNNGTG 1 cut(s) 346
Eam1104I CTCTTC 4 cut(s) 1143, 1245, 1468, 1735
EarI CTCTTC 4 cut(s) 1143, 1245, 1468, 1735
Ecl136II GAGCTC 1 cut(s) 918
Eco24I GRGCYC 2 cut(s) 778, 920
Eco47I GGWCC 1 cut(s) 264
Eco47III AGCGCT 1 cut(s) 909
Eco53kI GAGCTC 1 cut(s) 918
Eco57I CTGAAG 1 cut(s) 660
EcoICRI GAGCTC 1 cut(s) 918
EcoRII CCWGG 1 cut(s) 660
EcoT22I ATGCAT 2 cut(s) 1149, 1313
EcoT38I GRGCYC 2 cut(s) 778, 920
FalI AAGNNNNNCTT 2 cut(s) 168, 200
FauNDI CATATG 1 cut(s) 1083
FbaI TGATCA 1 cut(s) 843
FblI GTMKAC 2 cut(s) 212, 1750
FokI GGATG 2 cut(s) 1300, 1478
FriOI GRGCYC 2 cut(s) 778, 920
GlaI GCGC 2 cut(s) 909, 1695
HaeII RGCGCY 1 cut(s) 911
HaeIII GGCC 3 cut(s) 581, 650, 712
HapII CCGG 2 cut(s) 36, 1367
HgaI GACGC 2 cut(s) 881, 1259
HhaI GCGC 2 cut(s) 910, 1696
Hin6I GCGC 2 cut(s) 908, 1694
HinP1I GCGC 2 cut(s) 908, 1694
HinfI GANTC 8 cut(s) 40, 65, 122, 127, 717, 1117, 1557, 1634
HpaII CCGG 2 cut(s) 36, 1367
HphI GGTGA 1 cut(s) 21
Hpy166II GTNNAC 5 cut(s) 213, 632, 701, 790, 1751
Hpy188I TCNGA 3 cut(s) 547, 1526, 1720
Hpy8I GTNNAC 5 cut(s) 213, 632, 701, 790, 1751
HpyAV CCTTC 8 cut(s) 42, 320, 610, 684, 788, 864, 1285, 1621
HpyCH4III ACNGT 4 cut(s) 307, 543, 1261, 1339
HpyCH4IV ACGT 2 cut(s) 396, 622
HpyF10VI GCNNNNNNNGC 6 cut(s) 431, 524, 605, 773, 1097, 1691
HpyF3I CTNAG 1 cut(s) 1348
HpySE526I ACGT 2 cut(s) 396, 622
HspAI GCGC 2 cut(s) 908, 1694
Ksp22I TGATCA 1 cut(s) 843
Kzo9I GATC 3 cut(s) 437, 843, 1182
LguI GCTCTTC 2 cut(s) 1143, 1245
LmnI GCTCC 1 cut(s) 773
Lsp1109I GCAGC 8 cut(s) 332, 415, 514, 702, 887, 917, 1075, 1339
LweI GCATC 3 cut(s) 994, 1156, 1592
MaeII ACGT 2 cut(s) 396, 622
MaeIII GTNAC 4 cut(s) 557, 989, 1045, 1067
MalI GATC 3 cut(s) 439, 845, 1184
MboI GATC 3 cut(s) 437, 843, 1182
MfeI CAATTG 2 cut(s) 1112, 1680
MflI RGATCY 1 cut(s) 437
MhlI GDGCHC 2 cut(s) 778, 920
MlyI GAGTC 2 cut(s) 711, 1551
MmeI TCCRAC 1 cut(s) 1213
Mph1103I ATGCAT 2 cut(s) 1149, 1313
MroXI GAANNNNTTC 1 cut(s) 673
MseI TTAA 4 cut(s) 47, 186, 723, 1265
MslI CAYNNNNRTG 2 cut(s) 706, 1205
MspA1I CMGCKG 5 cut(s) 119, 239, 428, 878, 1352
MspCI CTTAAG 1 cut(s) 185
MspI CCGG 2 cut(s) 36, 1367
MspR9I CCNGG 3 cut(s) 37, 662, 1368
MunI CAATTG 2 cut(s) 1112, 1680
MvaI CCWGG 1 cut(s) 662
MvnI CGCG 1 cut(s) 1504
MwoI GCNNNNNNNGC 6 cut(s) 431, 524, 605, 773, 1097, 1691
NciI CCSGG 2 cut(s) 37, 1368
NdeI CATATG 1 cut(s) 1083
NdeII GATC 3 cut(s) 437, 843, 1182
NheI GCTAGC 2 cut(s) 56, 281
NlaIV GGNNCC 2 cut(s) 513, 775
NmuCI GTSAC 2 cut(s) 989, 1045
NsiI ATGCAT 2 cut(s) 1149, 1313
NspI RCATGY 6 cut(s) 26, 195, 747, 1315, 1726, 1777
NspV TTCGAA 1 cut(s) 125
OliI CACNNNNGTG 1 cut(s) 706
PaeI GCATGC 2 cut(s) 747, 1315
PciI ACATGT 3 cut(s) 22, 191, 1722
PciSI GCTCTTC 2 cut(s) 1143, 1245
PcsI WCGNNNNNNNCGW 1 cut(s) 1128
PdmI GAANNNNTTC 1 cut(s) 673
PfeI GAWTC 6 cut(s) 40, 65, 122, 127, 1117, 1634
PflFI GACNNNGTC 1 cut(s) 449
PfoI TCCNGGA 1 cut(s) 35
PleI GAGTC 2 cut(s) 711, 1551
PpsI GAGTC 2 cut(s) 711, 1551
Ppu21I YACGTR 1 cut(s) 623
PscI ACATGT 3 cut(s) 22, 191, 1722
PshAI GACNNNNGTC 1 cut(s) 145
Psp124BI GAGCTC 1 cut(s) 920
Psp6I CCWGG 1 cut(s) 660
PspGI CCWGG 1 cut(s) 660
PspN4I GGNNCC 2 cut(s) 513, 775
PspPI GGNCC 1 cut(s) 264
PstI CTGCAG 1 cut(s) 393
PstNI CAGNNNCTG 1 cut(s) 74
PsuI RGATCY 1 cut(s) 437
PsyI GACNNNGTC 1 cut(s) 449
PvuII CAGCTG 2 cut(s) 119, 1352
RsaI GTAC 2 cut(s) 566, 625
RsaNI GTAC 2 cut(s) 565, 624
RseI CAYNNNNRTG 2 cut(s) 706, 1205
SacI GAGCTC 1 cut(s) 920
SapI GCTCTTC 2 cut(s) 1143, 1245
SaqAI TTAA 4 cut(s) 47, 186, 723, 1265
Sau3AI GATC 3 cut(s) 437, 843, 1182
Sau96I GGNCC 1 cut(s) 264
SchI GAGTC 2 cut(s) 711, 1551
ScrFI CCNGG 3 cut(s) 37, 662, 1368
SduI GDGCHC 2 cut(s) 778, 920
SfaNI GCATC 3 cut(s) 994, 1156, 1592
SfcI CTRYAG 2 cut(s) 389, 1578
SfuI TTCGAA 1 cut(s) 125
SinI GGWCC 1 cut(s) 264
SmiMI CAYNNNNRTG 2 cut(s) 706, 1205
SmlI CTYRAG 4 cut(s) 185, 811, 1233, 1744
SmoI CTYRAG 4 cut(s) 185, 811, 1233, 1744
SpeI ACTAGT 2 cut(s) 626, 784
SphI GCATGC 2 cut(s) 747, 1315
SsiI CCGC 7 cut(s) 60, 237, 426, 431, 793, 878, 1504
SstI GAGCTC 1 cut(s) 920
StyD4I CCNGG 3 cut(s) 35, 660, 1366
TaaI ACNGT 4 cut(s) 307, 543, 1261, 1339
TaiI ACGT 2 cut(s) 399, 625
TaqI TCGA 6 cut(s) 125, 667, 958, 1150, 1203, 1586
TatI WGTACW 1 cut(s) 564
TauI GCSGC 4 cut(s) 63, 239, 428, 434
TfiI GAWTC 6 cut(s) 40, 65, 122, 127, 1117, 1634
Tru1I TTAA 4 cut(s) 47, 186, 723, 1265
Tru9I TTAA 4 cut(s) 47, 186, 723, 1265
TscAI CASTG 4 cut(s) 393, 415, 1303, 1612
TseFI GTSAC 2 cut(s) 989, 1045
TseI GCWGC 8 cut(s) 320, 428, 527, 690, 875, 905, 1088, 1352
Tsp45I GTSAC 2 cut(s) 989, 1045
TspDTI ATGAA 9 cut(s) 302, 315, 963, 1032, 1296, 1304, 1480, 1561, 1715
TspGWI ACGGA 1 cut(s) 1262
TspRI CASTG 4 cut(s) 393, 415, 1303, 1612
Tth111I GACNNNGTC 1 cut(s) 449
Vha464I CTTAAG 1 cut(s) 185
VpaK11BI GGWCC 1 cut(s) 264
XapI RAATTY 5 cut(s) 456, 574, 859, 1651, 1703
XbaI TCTAGA 3 cut(s) 290, 442, 1423
XceI RCATGY 6 cut(s) 26, 195, 747, 1315, 1726, 1777
XmiI GTMKAC 2 cut(s) 212, 1750
XmnI GAANNNNTTC 1 cut(s) 673
Zsp2I ATGCAT 2 cut(s) 1149, 1313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.