MD03G1146300.v1.1

Ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000157
Physical Location & Seq
Reverse (-)
16061493 .. 16067732
6240 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1146300.v1.1.491

Sequence Viewer

Length: 1662 bp
ATGCCCCTTTACAAAGCTGCAATGGAAGGCAACTGGGAAACCACCAAAGGCATCTTAAATATCAATCCGGCACTCTTAACTGCTAGCATAACAAAAGGATGGCAAACTGTTCTGCACGTTGCAGCAGGAGCAAAGAACAATATTCAGATTGTGGCAGAACTAGTCAAAATGATAGATGAAGACGACTTGGCACATCAAGACATTAAAGGAAATACGGCACTGAGTTTTGCCGCTGCAGCTGGAACCGTAGAAATTGCCGATATTTTGATACGAAAAAATAGGTTCTTGCCAACAATTCCAGGTGGCGAAGGAATGACACCACTTTATATGGCTGCTTTGTTTGGACAGTCTGAAATGGCAACGTATTTATACCCAAAAACGTATAAAATGTTGGATGAAATGGGCCGTAACGTTCTTTTTTTCTGTTGTATCGATCATGGTCTTTATGATTTAGCCATGAAGTTGCTACAAGATGATAGAACATTAGCAACGGCCCGTAAAAGCGACAAAGAAACGGCCTTACATGTCTTAGCTCGAAAGTCTTCAGAATTTGGTGACCAAAGTACTCCAGGAATGTGCAGAAGACTTATCAAAACACTTAAAGTACTGCCAGGGATGAATGTTTCTTACAAAAGAAACTTGAAGCAAACTCAAGCCCTGAATCTAGTCGAATGCCTTTGGAAAGAAATGTTGAAACATGACGACGACGAAGTGATGCTTCTGATAAGAGAACCTTCAGAATTAGTATTTGACGCTGCAAAATTAGGAAATTTTGAGTTCTTATCTGTGCTTATTGGCTTTTATCCGGAATTGCTATTAGAATATGATGACAACAATCGAACTATATTCCATATTGCAGTCTTGCATCGTCATGCAAGTATCTTTAATCTCGTGCATGTGACAGGCTCCATCAAAGATATCATAGCAACATTTACTGATGATGAGAATAACAACATTCTACATCTAGCTGCAAAACTGGCACCTCAAGACCAACTCAATCTTGTGTCCGGAGCAGCTCTTCAGATGCAACGAGAATTAGTATGGTTTGAGGAAGTCGAGAAGATTGTGCAACCTCCCTTTATAGATATGAAAAATAAGCAAGGCAAGACACCTCGAGAATTATTCACTAGCGAGCATGAGGATCTATTGCGCAAGGGAGAATCATGGATGAAGGACACTGCAAATTCATGCATGCTTGTTTCAACTCTTATCGCCACTGTCGTGTTTTCAGCCGCATTTAGCATTCCGGGAGGTATAAATGATCATAAAGGAACACCAAATTCCATAAAAGAGCCAGCCTTTTTAATATTCGCCATATCTGATGGAGTAGCACTCTTTTCCTCCTCAACTGCAATCCTCATGTTCTTGTACATCCTCACGTCACGGTATGCTGAAAACGATTTTCTCAAATCTTTACCGTTGAAGTTGATGATCGGGCTTACATCTCTCTTCGTGTCAATAACATCCATGATGGTAACTTTCAGCACAACCTTCTATTTATCTTGCCATGATGGATTAATATGGGTTCCAGATATTATATTCATATTTGCATTTGTTCCGGTTGCTTTGTTCGCTTTCTTACAGTATCCTCTCTTGTTTGATATGTTCTTTTCGACATATTATTCAAGTCTTTTGTTTCAGCCAAGGAGGGTTATGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

554

Amino Acids

62.15

Weight (kDa)

5.76

Isoelectric Point (pI)

39.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 3 - 92 1e-06 Ankyrin repeats (3 copies)
PGG PF13962 388 - 500 1.8e-27 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54070 AT5G35830
fragaria_vesca FvH4_3g25070 FvH4_3g25080 FvH4_3g25081 FvH4_3g25081
malus_domestica MD03G1027500.v1.1 MD03G1146300.v1.1 MD03G1146800.v1.1 MD03G1146900.v1.1 MD03G1147100.v1.1 MD11G1165600.v1.1
prunus_persica Prupe.2G015800_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G016300_v2.0.a1 Prupe.2G016500_v2.0.a1 Prupe.6G131100_v2.0.a1 Prupe.6G131400_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1
pyrus_communis pycom03g10090 pycom03g10110 pycom11g13740 pycom11g13770
rosa_chinensis RchiOBHm_Chr1g0316371 RchiOBHm_Chr1g0316381 RchiOBHm_Chr1g0316401 RchiOBHm_Chr1g0316411 RchiOBHm_Chr1g0316571 RchiOBHm_Chr1g0316601 RchiOBHm_Chr5g0039341 RchiOBHm_Chr5g0041371 RchiOBHm_Chr5g0041401 RchiOBHm_Chr5g0041431 RchiOBHm_Chr5g0041471 RchiOBHm_Chr5g0045281 RchiOBHm_Chr5g0045291 RchiOBHm_Chr5g0045311 RchiOBHm_Chr5g0045371 RchiOBHm_Chr5g0045421 RchiOBHm_Chr5g0045431 RchiOBHm_Chr5g0045501 RchiOBHm_Chr5g0045551 RchiOBHm_Chr5g0045771 RchiOBHm_Chr5g0045811
rosa_laevigata RLG00000034057 RLG00000034058
rosa_multiflora Rmu_co8352751.1_g000001 Rmu_co8397575.1_g000001 Rmu_sc0000415.1_g000006 Rmu_sc0000493.1_g000057 Rmu_sc0000504.1_g000003 Rmu_sc0000594.1_g000019 Rmu_sc0000594.1_g000032 Rmu_sc0000594.1_g000066 Rmu_sc0001047.1_g000003 Rmu_sc0001047.1_g000011 Rmu_sc0001556.1_g000023 Rmu_sc0002584.1_g000003 Rmu_sc0002584.1_g000039 Rmu_sc0002584.1_g000070 Rmu_sc0002667.1_g000015 Rmu_sc0003677.1_g000003 Rmu_sc0003743.1_g000017 Rmu_sc0004520.1_g000004 Rmu_sc0004520.1_g000013 Rmu_sc0004520.1_g000027 Rmu_sc0005539.1_g000014 Rmu_sc0005539.1_g000032 Rmu_sc0006018.1_g000006 Rmu_sc0006737.1_g000020 Rmu_sc0007485.1_g000002 Rmu_sc0008729.1_g000002 Rmu_sc0008729.1_g000003 Rmu_sc0038822.1_g000001 Rmu_ssc0000398.1_g000025
rosa_roxburghii Rroxscaffold_1G00035090 Rroxscaffold_1G00035630 Rroxscaffold_1G00035640 Rroxscaffold_1G00035660 Rroxscaffold_1G00035680 Rroxscaffold_1G00035730 Rroxscaffold_1G00035750 Rroxscaffold_1G00035770 Rroxscaffold_1G00039150 Rroxscaffold_1G00039190 Rroxscaffold_1G00039220 Rroxscaffold_1G00039260 Rroxscaffold_4G00328910 Rroxscaffold_4G00328950 Rroxscaffold_4G00328960 Rroxscaffold_4G00330890
rosa_rugosa Rorug01G0009800 Rorug01G0010000.1 Rorug01G0010200 Rorug01G0012100 Rorug01G0023500 Rorug01G0023600 Rorug01G0023700 Rorug01G0024700 Rorug05G0192000 Rorug05G0192200 Rorug05G0222400 Rorug05G0222500 Rorug05G0222600.1 Rorug05G0222700.1 Rorug05G0222800.1 Rorug05G0222900.1 Rorug05G0223000.1 Rorug05G0223100.1 Rorug05G0223200 Rorug05G0223600 Rorug05G0226800 Rorug05G0226900
rosa_samantha Rh1CG019600 Rh1DG015000 Rh1DG015300 Rh1DG016900 Rh5CG315200 Rh5CG338700 Rh5DG277100 Rh5DG291500 Rh5DG292100 Rh5DG292200 Rh5DG292400 Rh5DG292600 Rh5DG322100 Rh5DG322200 Rh5DG323100 Rh5DG323300 Rh5DG326700 Rh5DG326900 Rh5DG327000
rosa_wichuraiana Rw0G021580 Rw1G001320 Rw1G001420 Rw1G002820 Rw1G002840 Rw1G002870 Rw5G024800 Rw5G026160 Rw5G026170 Rw5G026180 Rw5G026210 Rw5G026230 Rw5G028170 Rw5G028190 Rw5G028210 Rw5G028230 Rw5G028240 Rw5G028260 Rw5G028280 Rw5G028370 Rw5G028380 Rw5G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1151
AccB1I GGYRCC 1 cut(s) 979
AccIII TCCGGA 2 cut(s) 805, 1007
AciI CCGC 2 cut(s) 231, 1233
AclI AACGTT 1 cut(s) 411
AclWI GGATC 1 cut(s) 1149
AcsI RAATTY 4 cut(s) 548, 769, 1183, 1279
AcuI CTGAAG 3 cut(s) 528, 720, 1004
AfaI GTAC 3 cut(s) 565, 606, 1370
AflIII ACRYGT 1 cut(s) 523
AgsI TTSAA 5 cut(s) 643, 694, 1203, 1423, 1626
AhlI ACTAGT 1 cut(s) 160
AjiI CACGTC 1 cut(s) 1380
AjnI CCWGG 3 cut(s) 298, 568, 610
AleI CACNNNNGTG 1 cut(s) 1220
AluBI AGCT 5 cut(s) 17, 239, 533, 968, 1016
AluI AGCT 5 cut(s) 17, 239, 533, 968, 1016
AlwI GGATC 1 cut(s) 1149
Ama87I CYCGRG 1 cut(s) 1113
Aor13HI TCCGGA 2 cut(s) 805, 1007
AoxI GGCC 3 cut(s) 403, 492, 516
ApeKI GCWGC 8 cut(s) 17, 122, 233, 236, 332, 755, 968, 1013
ApoI RAATTY 4 cut(s) 548, 769, 1183, 1279
AseI ATTAAT 1 cut(s) 1517
Asp700I GAANNNNTTC 1 cut(s) 541
AspLEI GCGC 1 cut(s) 1152
AspS9I GGNCC 2 cut(s) 403, 493
AsuC2I CCSGG 1 cut(s) 1248
AsuHPI GGTGA 1 cut(s) 566
AsuNHI GCTAGC 1 cut(s) 83
AvaI CYCGRG 1 cut(s) 1113
BanI GGYRCC 1 cut(s) 979
BauI CACGAG 1 cut(s) 890
BbsI GAAGAC 3 cut(s) 186, 534, 589
BbvI GCAGC 8 cut(s) 4, 134, 220, 248, 319, 742, 955, 1025
BccI CCATC 5 cut(s) 93, 917, 1316, 1465, 1505
BceAI ACGGC 4 cut(s) 231, 390, 507, 531
BciT130I CCWGG 3 cut(s) 300, 570, 612
BciVI GTATCC 1 cut(s) 1596
BclI TGATCA 1 cut(s) 1261
BcnI CCSGG 1 cut(s) 1248
BcuI ACTAGT 1 cut(s) 160
BfaI CTAG 5 cut(s) 84, 161, 665, 965, 1128
BfmI CTRYAG 1 cut(s) 234
BfuI GTATCC 1 cut(s) 1596
BmcAI AGTACT 2 cut(s) 565, 606
Bme1390I CCNGG 4 cut(s) 300, 570, 612, 1248
BmeT110I CYCGRG 1 cut(s) 1113
BmgBI CACGTC 1 cut(s) 1380
BmgT120I GGNCC 2 cut(s) 403, 493
BmiI GGNNCC 4 cut(s) 244, 907, 981, 1527
BmrFI CCNGG 4 cut(s) 300, 570, 612, 1248
BmrI ACTGGG 1 cut(s) 43
BmsI GCATC 4 cut(s) 60, 705, 874, 1014
BmtI GCTAGC 1 cut(s) 87
BmuI ACTGGG 1 cut(s) 43
BpiI GAAGAC 3 cut(s) 186, 534, 589
BplI GAGNNNNNCTC 2 cut(s) 1317, 1349
BpmI CTGGAG 1 cut(s) 552
BpuEI CTTGAG 2 cut(s) 636, 969
BpuMI CCSGG 1 cut(s) 1248
Bsa29I ATCGAT 1 cut(s) 432
BsaJI CCNNGG 2 cut(s) 611, 1643
BsaWI WCCGGW 3 cut(s) 805, 1007, 1558
Bse1I ACTGG 2 cut(s) 38, 981
Bse3DI GCAATG 1 cut(s) 27
BseAI TCCGGA 2 cut(s) 805, 1007
BseBI CCWGG 3 cut(s) 300, 570, 612
BseCI ATCGAT 1 cut(s) 432
BseDI CCNNGG 2 cut(s) 611, 1643
BseGI GGATG 6 cut(s) 104, 400, 621, 1173, 1371, 1463
BseMI GCAATG 1 cut(s) 27
BseMII CTCAG 1 cut(s) 212
BseNI ACTGG 2 cut(s) 38, 981
BseRI GAGGAG 1 cut(s) 1333
BseXI GCAGC 8 cut(s) 4, 134, 220, 248, 319, 742, 955, 1025
BsgI GTGCAG 2 cut(s) 98, 598
BshFI GGCC 3 cut(s) 405, 494, 518
BshNI GGYRCC 1 cut(s) 979
BshVI ATCGAT 1 cut(s) 432
BsiHKCI CYCGRG 1 cut(s) 1113
BsiSI CCGG 5 cut(s) 68, 806, 1008, 1247, 1559
BsmI GAATGC 2 cut(s) 677, 1242
BsnI GGCC 3 cut(s) 405, 494, 518
BsoBI CYCGRG 1 cut(s) 1113
Bsp13I TCCGGA 2 cut(s) 805, 1007
Bsp1407I TGTACA 1 cut(s) 1368
Bsp143I GATC 4 cut(s) 433, 1141, 1261, 1431
BspACI CCGC 2 cut(s) 231, 1233
BspANI GGCC 3 cut(s) 405, 494, 518
BspCNI CTCAG 1 cut(s) 213
BspDI ATCGAT 1 cut(s) 432
BspEI TCCGGA 2 cut(s) 805, 1007
BspLI GGNNCC 4 cut(s) 244, 907, 981, 1527
BspMAI CTGCAG 1 cut(s) 238
BspOI GCTAGC 1 cut(s) 87
BspPI GGATC 1 cut(s) 1149
BspQI GCTCTTC 1 cut(s) 1023
BspT107I GGYRCC 1 cut(s) 979
BsrDI GCAATG 1 cut(s) 27
BsrGI TGTACA 1 cut(s) 1368
BsrI ACTGG 2 cut(s) 38, 981
BssECI CCNNGG 2 cut(s) 611, 1643
BssMI GATC 4 cut(s) 433, 1141, 1261, 1431
BssSI CACGAG 1 cut(s) 890
BssT1I CCWWGG 1 cut(s) 1643
Bst2BI CACGAG 1 cut(s) 890
Bst2UI CCWGG 3 cut(s) 300, 570, 612
Bst4CI ACNGT 7 cut(s) 109, 247, 348, 1219, 1386, 1419, 1584
Bst6I CTCTTC 2 cut(s) 1023, 1454
BstAUI TGTACA 1 cut(s) 1368
BstC8I GCNNGC 4 cut(s) 85, 1133, 1193, 1296
BstDEI CTNAG 2 cut(s) 221, 529
BstEII GGTNACC 1 cut(s) 554
BstF5I GGATG 6 cut(s) 104, 400, 621, 1173, 1371, 1463
BstHHI GCGC 1 cut(s) 1152
BstKTI GATC 4 cut(s) 436, 1144, 1264, 1434
BstMBI GATC 4 cut(s) 433, 1141, 1261, 1431
BstMWI GCNNNNNNNGC 4 cut(s) 128, 236, 977, 1571
BstNI CCWGG 3 cut(s) 300, 570, 612
BstNSI RCATGY 3 cut(s) 527, 899, 1195
BstPI GGTNACC 1 cut(s) 554
BstSCI CCNGG 4 cut(s) 298, 568, 610, 1246
BstSFI CTRYAG 1 cut(s) 234
BstV1I GCAGC 8 cut(s) 4, 134, 220, 248, 319, 742, 955, 1025
BstV2I GAAGAC 3 cut(s) 186, 534, 589
BstX2I RGATCY 1 cut(s) 1141
BstYI RGATCY 1 cut(s) 1141
Bsu15I ATCGAT 1 cut(s) 432
BsuI GTATCC 1 cut(s) 1596
BsuRI GGCC 3 cut(s) 405, 494, 518
BsuTUI ATCGAT 1 cut(s) 432
BtrI CACGTC 1 cut(s) 1380
BtsCI GGATG 6 cut(s) 104, 400, 621, 1173, 1371, 1463
BtsI GCAGTG 1 cut(s) 1176
BtsIMutI CAGTG 3 cut(s) 218, 1176, 1215
Cac8I GCNNGC 4 cut(s) 85, 1133, 1193, 1296
CfoI GCGC 1 cut(s) 1152
Cfr13I GGNCC 2 cut(s) 403, 493
ClaI ATCGAT 1 cut(s) 432
CseI GACGC 1 cut(s) 761
Csp6I GTAC 3 cut(s) 564, 605, 1369
CviQI GTAC 3 cut(s) 564, 605, 1369
DdeI CTNAG 2 cut(s) 221, 529
DpnI GATC 4 cut(s) 435, 1143, 1263, 1433
DpnII GATC 4 cut(s) 433, 1141, 1261, 1431
Eam1104I CTCTTC 2 cut(s) 1023, 1454
EarI CTCTTC 2 cut(s) 1023, 1454
Eco130I CCWWGG 1 cut(s) 1643
Eco32I GATATC 1 cut(s) 919
Eco57I CTGAAG 3 cut(s) 528, 720, 1004
Eco88I CYCGRG 1 cut(s) 1113
Eco91I GGTNACC 1 cut(s) 554
EcoO65I GGTNACC 1 cut(s) 554
EcoRII CCWGG 3 cut(s) 298, 568, 610
EcoRV GATATC 1 cut(s) 919
EcoT14I CCWWGG 1 cut(s) 1643
EcoT22I ATGCAT 1 cut(s) 1193
ErhI CCWWGG 1 cut(s) 1643
FalI AAGNNNNNCTT 4 cut(s) 702, 734, 718, 750
FbaI TGATCA 1 cut(s) 1261
FokI GGATG 6 cut(s) 111, 407, 628, 1180, 1358, 1450
FspBI CTAG 5 cut(s) 84, 161, 665, 965, 1128
FspI TGCGCA 1 cut(s) 1151
GlaI GCGC 1 cut(s) 1151
GsuI CTGGAG 1 cut(s) 552
HaeIII GGCC 3 cut(s) 405, 494, 518
HapII CCGG 5 cut(s) 68, 806, 1008, 1247, 1559
HgaI GACGC 1 cut(s) 761
HhaI GCGC 1 cut(s) 1152
Hin6I GCGC 1 cut(s) 1150
HinP1I GCGC 1 cut(s) 1150
HinfI GANTC 2 cut(s) 661, 1160
HpaII CCGG 5 cut(s) 68, 806, 1008, 1247, 1559
HphI GGTGA 1 cut(s) 566
Hpy188I TCNGA 7 cut(s) 147, 352, 547, 723, 739, 1023, 1321
Hpy188III TCNNGA 7 cut(s) 197, 806, 986, 1008, 1057, 1115, 1529
Hpy99I CGWCG 2 cut(s) 707, 710
HpyAV CCTTC 5 cut(s) 20, 302, 744, 1165, 1501
HpyCH4III ACNGT 7 cut(s) 109, 247, 348, 1219, 1386, 1419, 1584
HpyCH4IV ACGT 5 cut(s) 117, 362, 380, 411, 1379
HpyF10VI GCNNNNNNNGC 4 cut(s) 128, 236, 977, 1571
HpyF3I CTNAG 2 cut(s) 221, 529
HpySE526I ACGT 5 cut(s) 117, 362, 380, 411, 1379
HspAI GCGC 1 cut(s) 1150
Kpn2I TCCGGA 2 cut(s) 805, 1007
Ksp22I TGATCA 1 cut(s) 1261
Kzo9I GATC 4 cut(s) 433, 1141, 1261, 1431
LguI GCTCTTC 1 cut(s) 1023
LmnI GCTCC 3 cut(s) 128, 911, 1010
Lsp1109I GCAGC 8 cut(s) 4, 134, 220, 248, 319, 742, 955, 1025
LweI GCATC 4 cut(s) 60, 705, 874, 1014
MaeI CTAG 5 cut(s) 84, 161, 665, 965, 1128
MaeII ACGT 5 cut(s) 117, 362, 380, 411, 1379
MaeIII GTNAC 5 cut(s) 407, 554, 898, 1380, 1474
MalI GATC 4 cut(s) 435, 1143, 1263, 1433
MboI GATC 4 cut(s) 433, 1141, 1261, 1431
MboII GAAGA 6 cut(s) 191, 534, 594, 1010, 1072, 1441
MflI RGATCY 1 cut(s) 1141
MmeI TCCRAC 1 cut(s) 372
Mph1103I ATGCAT 1 cut(s) 1193
MroI TCCGGA 2 cut(s) 805, 1007
MroXI GAANNNNTTC 1 cut(s) 541
MseI TTAA 8 cut(s) 56, 77, 204, 600, 885, 1304, 1517, 1660
MslI CAYNNNNRTG 2 cut(s) 870, 1220
MspA1I CMGCKG 2 cut(s) 233, 239
MspI CCGG 5 cut(s) 68, 806, 1008, 1247, 1559
MspR9I CCNGG 4 cut(s) 300, 570, 612, 1248
Mva1269I GAATGC 2 cut(s) 677, 1242
MvaI CCWGG 3 cut(s) 300, 570, 612
MwoI GCNNNNNNNGC 4 cut(s) 128, 236, 977, 1571
NciI CCSGG 1 cut(s) 1248
NdeII GATC 4 cut(s) 433, 1141, 1261, 1431
NheI GCTAGC 1 cut(s) 83
NlaIV GGNNCC 4 cut(s) 244, 907, 981, 1527
NmuCI GTSAC 3 cut(s) 554, 898, 1380
NsbI TGCGCA 1 cut(s) 1151
NsiI ATGCAT 1 cut(s) 1193
NspI RCATGY 3 cut(s) 527, 899, 1195
OliI CACNNNNGTG 1 cut(s) 1220
PaeI GCATGC 1 cut(s) 1195
PaeR7I CTCGAG 1 cut(s) 1113
PciI ACATGT 1 cut(s) 523
PciSI GCTCTTC 1 cut(s) 1023
PcsI WCGNNNNNNNCGW 1 cut(s) 1218
PctI GAATGC 2 cut(s) 677, 1242
PdmI GAANNNNTTC 1 cut(s) 541
PfeI GAWTC 2 cut(s) 661, 1160
PfoI TCCNGGA 2 cut(s) 568, 1246
PscI ACATGT 1 cut(s) 523
PshBI ATTAAT 1 cut(s) 1517
Psp1406I AACGTT 1 cut(s) 411
Psp6I CCWGG 3 cut(s) 298, 568, 610
PspEI GGTNACC 1 cut(s) 554
PspGI CCWGG 3 cut(s) 298, 568, 610
PspN4I GGNNCC 4 cut(s) 244, 907, 981, 1527
PspPI GGNCC 2 cut(s) 403, 493
PstI CTGCAG 1 cut(s) 238
PsuI RGATCY 1 cut(s) 1141
PvuII CAGCTG 1 cut(s) 239
RsaI GTAC 3 cut(s) 565, 606, 1370
RsaNI GTAC 3 cut(s) 564, 605, 1369
RseI CAYNNNNRTG 2 cut(s) 870, 1220
SapI GCTCTTC 1 cut(s) 1023
SaqAI TTAA 8 cut(s) 56, 77, 204, 600, 885, 1304, 1517, 1660
Sau3AI GATC 4 cut(s) 433, 1141, 1261, 1431
Sau96I GGNCC 2 cut(s) 403, 493
ScaI AGTACT 2 cut(s) 565, 606
ScrFI CCNGG 4 cut(s) 300, 570, 612, 1248
SfaNI GCATC 4 cut(s) 60, 705, 874, 1014
SfcI CTRYAG 1 cut(s) 234
Sfr274I CTCGAG 1 cut(s) 1113
SlaI CTCGAG 1 cut(s) 1113
SmiMI CAYNNNNRTG 2 cut(s) 870, 1220
SmlI CTYRAG 3 cut(s) 651, 984, 1113
SmoI CTYRAG 3 cut(s) 651, 984, 1113
SpeI ACTAGT 1 cut(s) 160
SphI GCATGC 1 cut(s) 1195
SsiI CCGC 2 cut(s) 231, 1233
SspI AATATT 2 cut(s) 142, 1308
SspMI CTAG 5 cut(s) 84, 161, 665, 965, 1128
StyD4I CCNGG 4 cut(s) 298, 568, 610, 1246
StyI CCWWGG 1 cut(s) 1643
TaaI ACNGT 7 cut(s) 109, 247, 348, 1219, 1386, 1419, 1584
TaiI ACGT 5 cut(s) 120, 365, 383, 414, 1382
TaqI TCGA 7 cut(s) 432, 535, 669, 838, 1056, 1114, 1613
TatI WGTACW 3 cut(s) 563, 604, 1368
TauI GCSGC 2 cut(s) 233, 1235
TfiI GAWTC 2 cut(s) 661, 1160
Tru1I TTAA 8 cut(s) 56, 77, 204, 600, 885, 1304, 1517, 1660
Tru9I TTAA 8 cut(s) 56, 77, 204, 600, 885, 1304, 1517, 1660
TscAI CASTG 3 cut(s) 225, 1183, 1222
TseFI GTSAC 3 cut(s) 554, 898, 1380
TseI GCWGC 8 cut(s) 17, 122, 233, 236, 332, 755, 968, 1013
Tsp45I GTSAC 3 cut(s) 554, 898, 1380
TspDTI ATGAA 8 cut(s) 192, 411, 473, 632, 1103, 1176, 1184, 1531
TspRI CASTG 3 cut(s) 225, 1183, 1222
VspI ATTAAT 1 cut(s) 1517
XapI RAATTY 4 cut(s) 548, 769, 1183, 1279
XceI RCATGY 3 cut(s) 527, 899, 1195
XhoI CTCGAG 1 cut(s) 1113
XmnI GAANNNNTTC 1 cut(s) 541
XspI CTAG 5 cut(s) 84, 161, 665, 965, 1128
ZrmI AGTACT 2 cut(s) 565, 606
Zsp2I ATGCAT 1 cut(s) 1193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.