Rroxscaffold_1G00035750

Ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000157
Physical Location & Seq
Forward (+)
52025432 .. 52039733
14302 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00035750.1

Sequence Viewer

Length: 2034 bp
ATGATAGCGAGCTCCATATACTTAAATTGTAAATTTCCTGCAGTGCTCTGTACATTGATCGACGACTACTTTCAGACCCCTATATATATCGCTTCAGACAGATTTTTACTGACTAAGCAAAAGGCTAGGAGAGTATTACTTGCTGCTTTAGCACCATTTCATATCGATCTGTGCCCACGCTGGTATATATGTTTATACAGTGTGTTCAATTTTCAATCTAAAACCATGTCTATCTCTCAAGAACAACATGTACCACCTGCTGGGATCCAATTAACCACCACCATCGATATTTCGGCAGATTTACCTAGACCTAAGACACCTTCACTTCATCTACTAGAACACAAAAATAGAGAGCTATACCTTAAGATATGTGTGCCTCTCTATAAGTATGCACTAAAAGGTGACTGGGAAGCTGCTGAACAAATCTTGGAAAAGGATTCGACACTTTTGAGTGCTAGCATATCAAGGGGATGGGACACTGTCCTTCATATTGCAGCAGGAGCAAGACATATTCACTTTGTGGAGAAACTGGTAGAAATTATGGACAAAGAACACTTGGCACTCCAAGACATAAATGGAAACACTGCACTATGCATTGCTGCAGCAGCAGGATCTGTAGAGATTGTCCAAATTTTGATAAATCATAATGATTCTTTGCTGACAGTTCGAGGAGGTCAAAAAATGGCACCACCGTATATGGCTGCTGTGTTAGGACAGTCACAAATGGCGTGGTACCTATACCCTAAAAGTAAAAAGATGTTGGAGAAAACCGACCTCGAAAATTTGTTCTTTTCGTGCATCAACAATGGTTTGTATGATTTAGCCTTACAGTTGCTGGATACTGATACAACATTAGCCAAGGCACGTACTGATAGGCCGAAAAATAAAACAGCCTTGCATATCTTGGCTAGAAGGCCTTCAGTATTTGGAACCCATAGTCCAGGAATGTGGGGTAGACTCATTAAATCAACCCTCCCAGGTTTTAAGTTTGCATTGAAAAGAAATTTAAATCATGTTGATGAAGCACTTCAGCTAGTCCAACGCCTTTGGGAAGAAATTTTGAAAGATGAGCATGATGACGTGATGAGGTTGATCAAATATCCCTCAAATTTATTATTTGATGCAACAGAATTAGGGAATTATGAGTTCCTAGCAGCGCTTATAAACTCATATCCTGAGTTAGTATGGGAAACTGATAAAAAAAATCGAACCATAATCCATGTCGCAGTTCTACATCGTCATGCAAGTATTTTCAATCTAGTGCATGAGATAGGGTCAATCAAGGATGTCATAGTGACTTATGAGGATGATCAGGGTAATAATATAGTGCATATGGCTGCAAAAGCAGCACCTCAAAACCAACTGAATCTAGTGTCGGGTGTAGCTCTTCAAATGCAGCGAGAAATAGTATGGTTTGAGGAAGTAAAGAAGATTGTGCAACCTCAATATATAGAGATGGAAAACAAGAATGGAAAAACACCTCAAGAAATATTCACTAAAGAGCATAAGACGTTAATGCATCAAGGAGAAAAATGGATGAAGGACACTGCAACTTCATGCTTGCTTGTTGCGACTATTATTGCAACTGTTGTGTTTTCAGCTGCATTTAGTATACCAGGTGGCACAGATGATCATACTGGAAAGCCAAACTTTTTAAAAGAAAAAGCGTTTTTATACTTTACCATAGCTGACGGAGTGGCACTTTTTTCCTCTTCAACTGCAATGCTGATGTTCTTGTTCATCCTTACCTCACGGTATGCAGAAAATGATTTCCTCATGTCCTTGCCCTTGAAGTTGATGGTTGGACTCACTTGCCTCTTCATCTCAATAGCATCTATGATGATGGCTTTTAGCACTGCCTTCTATTTGTCTTGTCAATATGGATCAAGATGGGTTCCAGATCTTACATTTTCTTTTGCAATTGTCCCTGTTGCTTTATATGCATTTCTGCAATTCCCTCTTGTGTCTGATATGTCGTCTTCAACATTTTGTTCAAGTCTTTTATTTCAGCCATGGAAACGTATAATATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

677

Amino Acids

76.58

Weight (kDa)

7.3

Isoelectric Point (pI)

45.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 131 - 216 3.6e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 162 - 249 2.8e-09 Ankyrin repeats (3 copies)
PGG PF13962 510 - 623 6.3e-26 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54070 AT5G35830
fragaria_vesca FvH4_3g25070 FvH4_3g25080 FvH4_3g25081 FvH4_3g25081
malus_domestica MD03G1027500.v1.1 MD03G1146300.v1.1 MD03G1146800.v1.1 MD03G1146900.v1.1 MD03G1147100.v1.1 MD11G1165600.v1.1
prunus_persica Prupe.2G015800_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G016300_v2.0.a1 Prupe.2G016500_v2.0.a1 Prupe.6G131100_v2.0.a1 Prupe.6G131400_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1
pyrus_communis pycom03g10090 pycom03g10110 pycom11g13740 pycom11g13770
rosa_chinensis RchiOBHm_Chr1g0316371 RchiOBHm_Chr1g0316381 RchiOBHm_Chr1g0316401 RchiOBHm_Chr1g0316411 RchiOBHm_Chr1g0316571 RchiOBHm_Chr1g0316601 RchiOBHm_Chr5g0039341 RchiOBHm_Chr5g0041371 RchiOBHm_Chr5g0041401 RchiOBHm_Chr5g0041431 RchiOBHm_Chr5g0041471 RchiOBHm_Chr5g0045281 RchiOBHm_Chr5g0045291 RchiOBHm_Chr5g0045311 RchiOBHm_Chr5g0045371 RchiOBHm_Chr5g0045421 RchiOBHm_Chr5g0045431 RchiOBHm_Chr5g0045501 RchiOBHm_Chr5g0045551 RchiOBHm_Chr5g0045771 RchiOBHm_Chr5g0045811
rosa_laevigata RLG00000034057 RLG00000034058
rosa_multiflora Rmu_co8352751.1_g000001 Rmu_co8397575.1_g000001 Rmu_sc0000415.1_g000006 Rmu_sc0000493.1_g000057 Rmu_sc0000504.1_g000003 Rmu_sc0000594.1_g000019 Rmu_sc0000594.1_g000032 Rmu_sc0000594.1_g000066 Rmu_sc0001047.1_g000003 Rmu_sc0001047.1_g000011 Rmu_sc0001556.1_g000023 Rmu_sc0002584.1_g000003 Rmu_sc0002584.1_g000039 Rmu_sc0002584.1_g000070 Rmu_sc0002667.1_g000015 Rmu_sc0003677.1_g000003 Rmu_sc0003743.1_g000017 Rmu_sc0004520.1_g000004 Rmu_sc0004520.1_g000013 Rmu_sc0004520.1_g000027 Rmu_sc0005539.1_g000014 Rmu_sc0005539.1_g000032 Rmu_sc0006018.1_g000006 Rmu_sc0006737.1_g000020 Rmu_sc0007485.1_g000002 Rmu_sc0008729.1_g000002 Rmu_sc0008729.1_g000003 Rmu_sc0038822.1_g000001 Rmu_ssc0000398.1_g000025
rosa_roxburghii Rroxscaffold_1G00035090 Rroxscaffold_1G00035630 Rroxscaffold_1G00035640 Rroxscaffold_1G00035660 Rroxscaffold_1G00035680 Rroxscaffold_1G00035730 Rroxscaffold_1G00035750 Rroxscaffold_1G00035770 Rroxscaffold_1G00039150 Rroxscaffold_1G00039190 Rroxscaffold_1G00039220 Rroxscaffold_1G00039260 Rroxscaffold_4G00328910 Rroxscaffold_4G00328950 Rroxscaffold_4G00328960 Rroxscaffold_4G00330890
rosa_rugosa Rorug01G0009800 Rorug01G0010000.1 Rorug01G0010200 Rorug01G0012100 Rorug01G0023500 Rorug01G0023600 Rorug01G0023700 Rorug01G0024700 Rorug05G0192000 Rorug05G0192200 Rorug05G0222400 Rorug05G0222500 Rorug05G0222600.1 Rorug05G0222700.1 Rorug05G0222800.1 Rorug05G0222900.1 Rorug05G0223000.1 Rorug05G0223100.1 Rorug05G0223200 Rorug05G0223600 Rorug05G0226800 Rorug05G0226900
rosa_samantha Rh1CG019600 Rh1DG015000 Rh1DG015300 Rh1DG016900 Rh5CG315200 Rh5CG338700 Rh5DG277100 Rh5DG291500 Rh5DG292100 Rh5DG292200 Rh5DG292400 Rh5DG292600 Rh5DG322100 Rh5DG322200 Rh5DG323100 Rh5DG323300 Rh5DG326700 Rh5DG326900 Rh5DG327000
rosa_wichuraiana Rw0G021580 Rw1G001320 Rw1G001420 Rw1G002820 Rw1G002840 Rw1G002870 Rw5G024800 Rw5G026160 Rw5G026170 Rw5G026180 Rw5G026210 Rw5G026230 Rw5G028170 Rw5G028190 Rw5G028210 Rw5G028230 Rw5G028240 Rw5G028260 Rw5G028280 Rw5G028370 Rw5G028380 Rw5G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1163
AarI CACCTGC 1 cut(s) 265
Acc36I ACCTGC 1 cut(s) 265
Acc65I GGTACC 1 cut(s) 732
AccB1I GGYRCC 2 cut(s) 685, 732
AccB7I CCANNNNNTGG 1 cut(s) 260
AccI GTMKAC 2 cut(s) 955, 1612
AclWI GGATC 4 cut(s) 259, 272, 619, 1891
AcsI RAATTY 6 cut(s) 32, 630, 781, 1003, 1056, 1108
AcuI CTGAAG 3 cut(s) 78, 903, 1013
AdeI CACNNNGTG 1 cut(s) 520
AfaI GTAC 4 cut(s) 52, 252, 734, 868
AfeI AGCGCT 1 cut(s) 1158
AfiI CCNNNNNNNGG 1 cut(s) 260
AflII CTTAAG 1 cut(s) 362
AflIII ACRYGT 1 cut(s) 247
AjiI CACGTC 1 cut(s) 1081
AjnI CCWGG 3 cut(s) 940, 976, 1615
AloI GAACNNNNNNTCC 2 cut(s) 922, 954
AluBI AGCT 7 cut(s) 12, 355, 413, 1033, 1385, 1601, 1688
AluI AGCT 7 cut(s) 12, 355, 413, 1033, 1385, 1601, 1688
Alw21I GWGCWC 2 cut(s) 14, 48
AlwI GGATC 4 cut(s) 259, 272, 619, 1891
AlwNI CAGNNNCTG 2 cut(s) 614, 835
Aor51HI AGCGCT 1 cut(s) 1158
AoxI GGCC 2 cut(s) 875, 914
ApoI RAATTY 6 cut(s) 32, 630, 781, 1003, 1056, 1108
Asp700I GAANNNNTTC 2 cut(s) 916, 1026
Asp718I GGTACC 1 cut(s) 732
AspLEI GCGC 1 cut(s) 1159
AsuHPI GGTGA 1 cut(s) 413
AsuNHI GCTAGC 1 cut(s) 455
BaeGI GKGCMC 1 cut(s) 176
BamHI GGATCC 1 cut(s) 264
BanI GGYRCC 2 cut(s) 685, 732
BanII GRGCYC 1 cut(s) 14
BbsI GAAGAC 1 cut(s) 1971
Bbv12I GWGCWC 2 cut(s) 14, 48
BccI CCATC 6 cut(s) 290, 465, 1450, 1792, 1837, 1884
BciT130I CCWGG 3 cut(s) 942, 978, 1617
BciVI GTATCC 1 cut(s) 832
BclI TGATCA 3 cut(s) 1092, 1309, 1630
BfaI CTAG 9 cut(s) 126, 306, 335, 456, 909, 1034, 1151, 1259, 1370
BfmI CTRYAG 3 cut(s) 39, 600, 615
BfoI RGCGCY 1 cut(s) 1160
BfrI CTTAAG 1 cut(s) 362
BfuAI ACCTGC 1 cut(s) 265
BfuI GTATCC 1 cut(s) 832
BglII AGATCT 1 cut(s) 1900
Bme1390I CCNGG 3 cut(s) 942, 978, 1617
BmgBI CACGTC 1 cut(s) 1081
BmiI GGNNCC 5 cut(s) 266, 687, 734, 931, 1896
BmrFI CCNGG 3 cut(s) 942, 978, 1617
BmrI ACTGGG 1 cut(s) 415
BmsI GCATC 4 cut(s) 807, 1111, 1528, 1841
BmtI GCTAGC 1 cut(s) 459
BmuI ACTGGG 1 cut(s) 415
BpiI GAAGAC 1 cut(s) 1971
BpuEI CTTGAG 2 cut(s) 222, 1467
Bsa29I ATCGAT 2 cut(s) 165, 285
BsaAI YACGTR 1 cut(s) 866
BsaJI CCNNGG 3 cut(s) 858, 976, 2012
Bsc4I CCNNNNNNNGG 1 cut(s) 260
Bse1I ACTGG 3 cut(s) 410, 534, 1642
Bse3DI GCAATG 2 cut(s) 594, 1728
BseBI CCWGG 3 cut(s) 942, 978, 1617
BseCI ATCGAT 2 cut(s) 165, 285
BseDI CCNNGG 3 cut(s) 858, 976, 2012
BseGI GGATG 5 cut(s) 476, 1291, 1312, 1542, 1740
BseLI CCNNNNNNNGG 1 cut(s) 260
BseMI GCAATG 2 cut(s) 594, 1728
BseMII CTCAG 1 cut(s) 1167
BseNI ACTGG 3 cut(s) 410, 534, 1642
BseRI GAGGAG 1 cut(s) 684
BseSI GKGCMC 1 cut(s) 176
BseYI CCCAGC 1 cut(s) 260
BsgI GTGCAG 1 cut(s) 570
BshFI GGCC 2 cut(s) 877, 916
BshNI GGYRCC 2 cut(s) 685, 732
BshVI ATCGAT 2 cut(s) 165, 285
BsiHKAI GWGCWC 2 cut(s) 14, 48
BslFI GGGAC 2 cut(s) 488, 1910
BslI CCNNNNNNNGG 1 cut(s) 260
BsmFI GGGAC 2 cut(s) 488, 1910
BsnI GGCC 2 cut(s) 877, 916
Bsp1286I GDGCHC 3 cut(s) 14, 48, 176
Bsp1407I TGTACA 1 cut(s) 50
Bsp143I GATC 9 cut(s) 57, 166, 264, 611, 1092, 1309, 1630, 1883, 1900
Bsp19I CCATGG 1 cut(s) 2012
BspANI GGCC 2 cut(s) 877, 916
BspCNI CTCAG 1 cut(s) 1168
BspDI ATCGAT 2 cut(s) 165, 285
BspLI GGNNCC 5 cut(s) 266, 687, 734, 931, 1896
BspMAI CTGCAG 2 cut(s) 43, 604
BspMI ACCTGC 1 cut(s) 265
BspOI GCTAGC 1 cut(s) 459
BspPI GGATC 4 cut(s) 259, 272, 619, 1891
BspQI GCTCTTC 1 cut(s) 1392
BspT107I GGYRCC 2 cut(s) 685, 732
BspTI CTTAAG 1 cut(s) 362
BsrDI GCAATG 2 cut(s) 594, 1728
BsrGI TGTACA 1 cut(s) 50
BsrI ACTGG 3 cut(s) 410, 534, 1642
BssECI CCNNGG 3 cut(s) 858, 976, 2012
BssMI GATC 9 cut(s) 57, 166, 264, 611, 1092, 1309, 1630, 1883, 1900
BssNAI GTATAC 1 cut(s) 1613
BssT1I CCWWGG 2 cut(s) 858, 2012
Bst1107I GTATAC 1 cut(s) 1613
Bst2UI CCWGG 3 cut(s) 942, 978, 1617
Bst4CI ACNGT 8 cut(s) 200, 481, 664, 693, 717, 831, 1588, 1755
Bst6I CTCTTC 3 cut(s) 1392, 1717, 1823
BstAFI CTTAAG 1 cut(s) 362
BstAUI TGTACA 1 cut(s) 50
BstBAI YACGTR 1 cut(s) 866
BstC8I GCNNGC 3 cut(s) 10, 457, 1562
BstDEI CTNAG 3 cut(s) 114, 312, 1176
BstDSI CCRYGG 1 cut(s) 2012
BstF5I GGATG 5 cut(s) 476, 1291, 1312, 1542, 1740
BstH2I RGCGCY 1 cut(s) 1160
BstHHI GCGC 1 cut(s) 1159
BstKTI GATC 9 cut(s) 60, 169, 267, 614, 1095, 1312, 1633, 1886, 1903
BstMBI GATC 9 cut(s) 57, 166, 264, 611, 1092, 1309, 1630, 1883, 1900
BstMWI GCNNNNNNNGC 6 cut(s) 149, 500, 605, 1343, 1346, 1940
BstNI CCWGG 3 cut(s) 942, 978, 1617
BstNSI RCATGY 1 cut(s) 251
BstSCI CCNGG 3 cut(s) 940, 976, 1615
BstSFI CTRYAG 3 cut(s) 39, 600, 615
BstSLI GKGCMC 1 cut(s) 176
BstV2I GAAGAC 1 cut(s) 1971
BstX2I RGATCY 3 cut(s) 264, 611, 1900
BstXI CCANNNNNNTGG 1 cut(s) 948
BstYI RGATCY 3 cut(s) 264, 611, 1900
BstZ17I GTATAC 1 cut(s) 1613
Bsu15I ATCGAT 2 cut(s) 165, 285
BsuI GTATCC 1 cut(s) 832
BsuRI GGCC 2 cut(s) 877, 916
BsuTUI ATCGAT 2 cut(s) 165, 285
BtgI CCRYGG 1 cut(s) 2012
BtrI CACGTC 1 cut(s) 1081
BtsCI GGATG 5 cut(s) 476, 1291, 1312, 1542, 1740
BtsI GCAGTG 4 cut(s) 48, 582, 1545, 1854
BtsIMutI CAGTG 6 cut(s) 48, 205, 477, 582, 1545, 1854
BveI ACCTGC 1 cut(s) 265
Cac8I GCNNGC 3 cut(s) 10, 457, 1562
CaiI CAGNNNCTG 2 cut(s) 614, 835
CfoI GCGC 1 cut(s) 1159
ClaI ATCGAT 2 cut(s) 165, 285
CsiI ACCWGGT 1 cut(s) 1615
Csp6I GTAC 4 cut(s) 51, 251, 733, 867
CspCI CAANNNNNGTGG 2 cut(s) 710, 745
CviQI GTAC 4 cut(s) 51, 251, 733, 867
DdeI CTNAG 3 cut(s) 114, 312, 1176
DpnI GATC 9 cut(s) 59, 168, 266, 613, 1094, 1311, 1632, 1885, 1902
DpnII GATC 9 cut(s) 57, 166, 264, 611, 1092, 1309, 1630, 1883, 1900
DraI TTTAAA 2 cut(s) 1008, 1656
DraIII CACNNNGTG 1 cut(s) 520
Eam1104I CTCTTC 3 cut(s) 1392, 1717, 1823
EarI CTCTTC 3 cut(s) 1392, 1717, 1823
Ecl136II GAGCTC 1 cut(s) 12
Eco130I CCWWGG 2 cut(s) 858, 2012
Eco147I AGGCCT 1 cut(s) 916
Eco24I GRGCYC 1 cut(s) 14
Eco47III AGCGCT 1 cut(s) 1158
Eco53kI GAGCTC 1 cut(s) 12
Eco57I CTGAAG 3 cut(s) 78, 903, 1013
EcoICRI GAGCTC 1 cut(s) 12
EcoRII CCWGG 3 cut(s) 940, 976, 1615
EcoT14I CCWWGG 2 cut(s) 858, 2012
EcoT22I ATGCAT 3 cut(s) 596, 1521, 1945
EcoT38I GRGCYC 1 cut(s) 14
ErhI CCWWGG 2 cut(s) 858, 2012
FalI AAGNNNNNCTT 2 cut(s) 1634, 1666
FaqI GGGAC 2 cut(s) 488, 1910
FauNDI CATATG 1 cut(s) 1332
FbaI TGATCA 3 cut(s) 1092, 1309, 1630
FblI GTMKAC 2 cut(s) 955, 1612
FokI GGATG 5 cut(s) 483, 1298, 1319, 1549, 1727
FriOI GRGCYC 1 cut(s) 14
FspBI CTAG 9 cut(s) 126, 306, 335, 456, 909, 1034, 1151, 1259, 1370
GlaI GCGC 1 cut(s) 1158
GsaI CCCAGC 1 cut(s) 264
HaeII RGCGCY 1 cut(s) 1160
HaeIII GGCC 2 cut(s) 877, 916
HhaI GCGC 1 cut(s) 1159
Hin6I GCGC 1 cut(s) 1157
HinP1I GCGC 1 cut(s) 1157
HinfI GANTC 5 cut(s) 437, 650, 957, 1366, 1806
HphI GGTGA 1 cut(s) 413
Hpy166II GTNNAC 2 cut(s) 956, 1613
Hpy188I TCNGA 3 cut(s) 75, 97, 1969
Hpy188III TCNNGA 5 cut(s) 239, 1175, 1484, 1887, 1898
Hpy8I GTNNAC 2 cut(s) 956, 1613
Hpy99I CGWCG 1 cut(s) 65
HpyAV CCTTC 6 cut(s) 330, 494, 906, 927, 1534, 1870
HpyCH4III ACNGT 8 cut(s) 200, 481, 664, 693, 717, 831, 1588, 1755
HpyCH4IV ACGT 4 cut(s) 865, 1080, 1511, 2020
HpyF10VI GCNNNNNNNGC 6 cut(s) 149, 500, 605, 1343, 1346, 1940
HpyF3I CTNAG 3 cut(s) 114, 312, 1176
HpySE526I ACGT 4 cut(s) 865, 1080, 1511, 2020
HspAI GCGC 1 cut(s) 1157
KpnI GGTACC 1 cut(s) 736
Ksp22I TGATCA 3 cut(s) 1092, 1309, 1630
Kzo9I GATC 9 cut(s) 57, 166, 264, 611, 1092, 1309, 1630, 1883, 1900
LguI GCTCTTC 1 cut(s) 1392
LmnI GCTCC 2 cut(s) 17, 500
LweI GCATC 4 cut(s) 807, 1111, 1528, 1841
MabI ACCWGGT 1 cut(s) 1615
MaeI CTAG 9 cut(s) 126, 306, 335, 456, 909, 1034, 1151, 1259, 1370
MaeII ACGT 4 cut(s) 865, 1080, 1511, 2020
MaeIII GTNAC 3 cut(s) 401, 717, 1294
MalI GATC 9 cut(s) 59, 168, 266, 613, 1094, 1311, 1632, 1885, 1902
MboI GATC 9 cut(s) 57, 166, 264, 611, 1092, 1309, 1630, 1883, 1900
MboII GAAGA 6 cut(s) 1064, 1379, 1441, 1704, 1810, 1971
MfeI CAATTG 1 cut(s) 1920
MflI RGATCY 3 cut(s) 264, 611, 1900
MhlI GDGCHC 3 cut(s) 14, 48, 176
MlyI GAGTC 2 cut(s) 951, 1800
MmeI TCCRAC 3 cut(s) 741, 1063, 1783
Mph1103I ATGCAT 3 cut(s) 596, 1521, 1945
MroXI GAANNNNTTC 2 cut(s) 916, 1026
MseI TTAA 8 cut(s) 23, 272, 363, 963, 984, 1007, 1514, 1655
MslI CAYNNNNRTG 2 cut(s) 1017, 1239
MspA1I CMGCKG 1 cut(s) 1601
MspCI CTTAAG 1 cut(s) 362
MspR9I CCNGG 3 cut(s) 942, 978, 1617
MunI CAATTG 1 cut(s) 1920
MvaI CCWGG 3 cut(s) 942, 978, 1617
MwoI GCNNNNNNNGC 6 cut(s) 149, 500, 605, 1343, 1346, 1940
NcoI CCATGG 1 cut(s) 2012
NdeI CATATG 1 cut(s) 1332
NdeII GATC 9 cut(s) 57, 166, 264, 611, 1092, 1309, 1630, 1883, 1900
NheI GCTAGC 1 cut(s) 455
NlaIV GGNNCC 5 cut(s) 266, 687, 734, 931, 1896
NmuCI GTSAC 3 cut(s) 401, 717, 1294
NsiI ATGCAT 3 cut(s) 596, 1521, 1945
NspI RCATGY 1 cut(s) 251
PaqCI CACCTGC 1 cut(s) 265
PceI AGGCCT 1 cut(s) 916
PciI ACATGT 1 cut(s) 247
PciSI GCTCTTC 1 cut(s) 1392
PdmI GAANNNNTTC 2 cut(s) 916, 1026
PfeI GAWTC 3 cut(s) 437, 650, 1366
PflFI GACNNNGTC 1 cut(s) 479
PflMI CCANNNNNTGG 1 cut(s) 260
PfoI TCCNGGA 1 cut(s) 940
PleI GAGTC 2 cut(s) 951, 1800
PpsI GAGTC 2 cut(s) 951, 1800
Ppu21I YACGTR 1 cut(s) 866
PscI ACATGT 1 cut(s) 247
PsiI TTATAA 1 cut(s) 1163
Psp124BI GAGCTC 1 cut(s) 14
Psp6I CCWGG 3 cut(s) 940, 976, 1615
PspFI CCCAGC 1 cut(s) 260
PspGI CCWGG 3 cut(s) 940, 976, 1615
PspN4I GGNNCC 5 cut(s) 266, 687, 734, 931, 1896
PsrI GAACNNNNNNTAC 2 cut(s) 234, 266
PstI CTGCAG 2 cut(s) 43, 604
PstNI CAGNNNCTG 2 cut(s) 614, 835
PsuI RGATCY 3 cut(s) 264, 611, 1900
PsyI GACNNNGTC 1 cut(s) 479
PvuII CAGCTG 1 cut(s) 1601
RsaI GTAC 4 cut(s) 52, 252, 734, 868
RsaNI GTAC 4 cut(s) 51, 251, 733, 867
RseI CAYNNNNRTG 2 cut(s) 1017, 1239
SacI GAGCTC 1 cut(s) 14
SapI GCTCTTC 1 cut(s) 1392
SaqAI TTAA 8 cut(s) 23, 272, 363, 963, 984, 1007, 1514, 1655
Sau3AI GATC 9 cut(s) 57, 166, 264, 611, 1092, 1309, 1630, 1883, 1900
SchI GAGTC 2 cut(s) 951, 1800
ScrFI CCNGG 3 cut(s) 942, 978, 1617
SduI GDGCHC 3 cut(s) 14, 48, 176
SexAI ACCWGGT 1 cut(s) 1615
SfaNI GCATC 4 cut(s) 807, 1111, 1528, 1841
SfcI CTRYAG 3 cut(s) 39, 600, 615
SmiI ATTTAAAT 1 cut(s) 1008
SmiMI CAYNNNNRTG 2 cut(s) 1017, 1239
SmlI CTYRAG 3 cut(s) 237, 362, 1482
SmoI CTYRAG 3 cut(s) 237, 362, 1482
SseBI AGGCCT 1 cut(s) 916
SspI AATATT 1 cut(s) 1491
SspMI CTAG 9 cut(s) 126, 306, 335, 456, 909, 1034, 1151, 1259, 1370
SstI GAGCTC 1 cut(s) 14
StuI AGGCCT 1 cut(s) 916
StyD4I CCNGG 3 cut(s) 940, 976, 1615
StyI CCWWGG 2 cut(s) 858, 2012
SwaI ATTTAAAT 1 cut(s) 1008
TaaI ACNGT 8 cut(s) 200, 481, 664, 693, 717, 831, 1588, 1755
TaiI ACGT 4 cut(s) 868, 1083, 1514, 2023
TaqI TCGA 7 cut(s) 60, 165, 285, 440, 667, 777, 1207
TatI WGTACW 1 cut(s) 50
TfiI GAWTC 3 cut(s) 437, 650, 1366
Tru1I TTAA 8 cut(s) 23, 272, 363, 963, 984, 1007, 1514, 1655
Tru9I TTAA 8 cut(s) 23, 272, 363, 963, 984, 1007, 1514, 1655
TscAI CASTG 6 cut(s) 48, 205, 484, 589, 1552, 1861
TseFI GTSAC 3 cut(s) 401, 717, 1294
Tsp45I GTSAC 3 cut(s) 401, 717, 1294
TspDTI ATGAA 8 cut(s) 149, 317, 476, 1035, 1545, 1553, 1729, 1810
TspGWI ACGGA 1 cut(s) 1707
TspRI CASTG 6 cut(s) 48, 205, 484, 589, 1552, 1861
Tth111I GACNNNGTC 1 cut(s) 479
Van91I CCANNNNNTGG 1 cut(s) 260
Vha464I CTTAAG 1 cut(s) 362
XapI RAATTY 6 cut(s) 32, 630, 781, 1003, 1056, 1108
XceI RCATGY 1 cut(s) 251
XcmI CCANNNNNNNNNTGG 1 cut(s) 572
XmiI GTMKAC 2 cut(s) 955, 1612
XmnI GAANNNNTTC 2 cut(s) 916, 1026
XspI CTAG 9 cut(s) 126, 306, 335, 456, 909, 1034, 1151, 1259, 1370
Zsp2I ATGCAT 3 cut(s) 596, 1521, 1945
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.