Rroxscaffold_1G00035770

Ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000157
Physical Location & Seq
Forward (+)
52065375 .. 52069615
4241 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00035770.1

Sequence Viewer

Length: 1968 bp
ATGCAAGTGCTCCATATTGATGGTGACGACTCCTATACATATCCTCTTCCAGTTTCCACACTAGACATATCCTCTTCCAGTTTCCACACTAGGTGCATGCATGCTTGTATCTTTACACAATTTGTTCAATCTGGAACCATGGCTACCCCACAAGCACAACATATATCACCTGCCGGGATTCAATTAACCACGGCTAGCGGGAATTCAGCAACTGGGATAGATAGTCAATCAGCGGACATTGGCATCGACATTTCGGCTGATTCGGTTCAACCTACCAGACCTTTGCCTTATCTGCTAGAACATATAAATAGAGAAGAATACCTTAAGACATGTGTTGCCCTCTATAAGTATGCACTAAAGGGCGATTGGGAAGCTGCGGAACGAATCTTGAAAAAGGATACGAAACTTCTGAGAGCAAGCATATCTAGAGGATATGAAACAGTCCTTCACGTTGCAGCAGGAGCAAGACGTATTCATTTTGTGGAGAAACTGATCAGCATGATGAACGTGGAGGATTTGGCACTGCAAGACGTAAATGGAAACACTGCCTTGTGTATTGCCGCTGCGGCTGGATCTCTAGACATTGTCCAGATTTTGATACAAAAGAATAAGTCTTTACTATCGATTCGAGGCGGTAAAGATATGACACCAGCCTACATGGCTGTTGTGTTGGGACAGTCAGAAATGGCATGGCACCTGTACGCTGAAAATTTGGCCTTACCGTTGCTAAATGATGATAGAACATTAGCGACTGCACGTACTAAATCGCAGAAATATAAAACAGCCTTGCATATATTGGCTAGAAGGCCTTCAGCATTTGGTGGCAGCCAAAGTCCAGGAATGTGGAGTAGACTTACTAACTCATTCCTCCCAAGTTTGAAGTTTGCTTGCAAAAGAAAGTTAAAACAAGCTAATGAAGCCCTTCAACTAGTCCAACGTCTTTGGGAAGAAATTTTAAAAGATGAGCACGAAGACGTGATGAGGTTGATCAAATATCCTTCAAATTTATTATTTGACGCAGCAGAATTAGGGAATTATGAGTTTCTGGCGGCACTTATGAGCTCTTATCCTGAGTTGGTATGGGAAACTGATGAAAAAAATCGAACCATTATCCATGTCGCAGTTCTGCATCGTCATGCAAGTATCTTTAATCTAGTGCATGAAATAGGCTCGATCAAAGATGTCATAGTGACATATGAGGATGATCAGGGTAACAACATTGTGCATATGGCTGCAAAATTAGCACCTCAAAATCAGTTGAATCTAGTGTCAGGCGTAGCTCTTCAAATGCATCGAGAGTTGGTATGGTTTGAGGAAGTCAAAAAAATTGTTCAACCTCAGTATATAGAGATGGAAAACCAACAAGGAAAAACACCTCAAGAATTATTCACTAAGGAGCATAGGGCGCTAATGCGTCAAGGAGAATCATGGATGAAAGACACTGCAACTTCATGCTTGCTCGTTGCAACTATTATTGCAACTGTTGTGTTTTCAGCTGCATTTAGCATACCAGGTGGCACAGATGATCATACCGGAAAGCCCAACTTCTTAAAAGAGAAAATGTTTTTATACTTTACCATAGCCGATGGACTAGCACTCTTTTCCTCTTCAACTGCAATGCTAATGTTCTTGTTCATCCTAACCTCGCGGTACGCAGAAAACGATTTTCTCAAATCCTTACCATTGAAGTTGATGATAGGACTCACTTCCCTGTTCATCTCTATAGCATCCATGATGATGGCTTTCAGCACCGCCTTCTATTTGTCTTGTCAATATGAATCAAGATGGGTTTCAGATTTTACATTTTTATTTGCACTTGTCCCAGTTGCTTTGTTTGCGTTTATGCAATTCCCTCTCGTGTCCGACATGTCCTCTTCTATATTTTGCTCAAGTTTGTTATTTCAGCCGTGGGAAACACATGCTCTACTAGAAGGCAAACTGAATGTTGTACATATTGTACTTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

655

Amino Acids

73.27

Weight (kDa)

6.29

Isoelectric Point (pI)

45.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 118 - 203 4.6e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 149 - 234 2.2e-09 Ankyrin repeats (3 copies)
PGG PF13962 476 - 588 2.2e-25 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54070 AT5G35830
fragaria_vesca FvH4_3g25070 FvH4_3g25080 FvH4_3g25081 FvH4_3g25081
malus_domestica MD03G1027500.v1.1 MD03G1146300.v1.1 MD03G1146800.v1.1 MD03G1146900.v1.1 MD03G1147100.v1.1 MD11G1165600.v1.1
prunus_persica Prupe.2G015800_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G016300_v2.0.a1 Prupe.2G016500_v2.0.a1 Prupe.6G131100_v2.0.a1 Prupe.6G131400_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1
pyrus_communis pycom03g10090 pycom03g10110 pycom11g13740 pycom11g13770
rosa_chinensis RchiOBHm_Chr1g0316371 RchiOBHm_Chr1g0316381 RchiOBHm_Chr1g0316401 RchiOBHm_Chr1g0316411 RchiOBHm_Chr1g0316571 RchiOBHm_Chr1g0316601 RchiOBHm_Chr5g0039341 RchiOBHm_Chr5g0041371 RchiOBHm_Chr5g0041401 RchiOBHm_Chr5g0041431 RchiOBHm_Chr5g0041471 RchiOBHm_Chr5g0045281 RchiOBHm_Chr5g0045291 RchiOBHm_Chr5g0045311 RchiOBHm_Chr5g0045371 RchiOBHm_Chr5g0045421 RchiOBHm_Chr5g0045431 RchiOBHm_Chr5g0045501 RchiOBHm_Chr5g0045551 RchiOBHm_Chr5g0045771 RchiOBHm_Chr5g0045811
rosa_laevigata RLG00000034057 RLG00000034058
rosa_multiflora Rmu_co8352751.1_g000001 Rmu_co8397575.1_g000001 Rmu_sc0000415.1_g000006 Rmu_sc0000493.1_g000057 Rmu_sc0000504.1_g000003 Rmu_sc0000594.1_g000019 Rmu_sc0000594.1_g000032 Rmu_sc0000594.1_g000066 Rmu_sc0001047.1_g000003 Rmu_sc0001047.1_g000011 Rmu_sc0001556.1_g000023 Rmu_sc0002584.1_g000003 Rmu_sc0002584.1_g000039 Rmu_sc0002584.1_g000070 Rmu_sc0002667.1_g000015 Rmu_sc0003677.1_g000003 Rmu_sc0003743.1_g000017 Rmu_sc0004520.1_g000004 Rmu_sc0004520.1_g000013 Rmu_sc0004520.1_g000027 Rmu_sc0005539.1_g000014 Rmu_sc0005539.1_g000032 Rmu_sc0006018.1_g000006 Rmu_sc0006737.1_g000020 Rmu_sc0007485.1_g000002 Rmu_sc0008729.1_g000002 Rmu_sc0008729.1_g000003 Rmu_sc0038822.1_g000001 Rmu_ssc0000398.1_g000025
rosa_roxburghii Rroxscaffold_1G00035090 Rroxscaffold_1G00035630 Rroxscaffold_1G00035640 Rroxscaffold_1G00035660 Rroxscaffold_1G00035680 Rroxscaffold_1G00035730 Rroxscaffold_1G00035750 Rroxscaffold_1G00035770 Rroxscaffold_1G00039150 Rroxscaffold_1G00039190 Rroxscaffold_1G00039220 Rroxscaffold_1G00039260 Rroxscaffold_4G00328910 Rroxscaffold_4G00328950 Rroxscaffold_4G00328960 Rroxscaffold_4G00330890
rosa_rugosa Rorug01G0009800 Rorug01G0010000.1 Rorug01G0010200 Rorug01G0012100 Rorug01G0023500 Rorug01G0023600 Rorug01G0023700 Rorug01G0024700 Rorug05G0192000 Rorug05G0192200 Rorug05G0222400 Rorug05G0222500 Rorug05G0222600.1 Rorug05G0222700.1 Rorug05G0222800.1 Rorug05G0222900.1 Rorug05G0223000.1 Rorug05G0223100.1 Rorug05G0223200 Rorug05G0223600 Rorug05G0226800 Rorug05G0226900
rosa_samantha Rh1CG019600 Rh1DG015000 Rh1DG015300 Rh1DG016900 Rh5CG315200 Rh5CG338700 Rh5DG277100 Rh5DG291500 Rh5DG292100 Rh5DG292200 Rh5DG292400 Rh5DG292600 Rh5DG322100 Rh5DG322200 Rh5DG323100 Rh5DG323300 Rh5DG326700 Rh5DG326900 Rh5DG327000
rosa_wichuraiana Rw0G021580 Rw1G001320 Rw1G001420 Rw1G002820 Rw1G002840 Rw1G002870 Rw5G024800 Rw5G026160 Rw5G026170 Rw5G026180 Rw5G026210 Rw5G026230 Rw5G028170 Rw5G028190 Rw5G028210 Rw5G028230 Rw5G028240 Rw5G028260 Rw5G028280 Rw5G028370 Rw5G028380 Rw5G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 178
Acc36I ACCTGC 1 cut(s) 178
AccB1I GGYRCC 1 cut(s) 693
AccI GTMKAC 1 cut(s) 850
AccII CGCG 1 cut(s) 1648
AciI CCGC 9 cut(s) 198, 233, 377, 561, 566, 633, 1049, 1648, 1752
AclWI GGATC 1 cut(s) 580
AcsI RAATTY 4 cut(s) 202, 709, 951, 1003
AcuI CTGAAG 1 cut(s) 795
AdeI CACNNNGTG 1 cut(s) 93
AfaI GTAC 5 cut(s) 701, 760, 1652, 1950, 1959
AflII CTTAAG 2 cut(s) 323, 1961
AflIII ACRYGT 2 cut(s) 329, 1866
AhlI ACTAGT 1 cut(s) 928
AjiI CACGTC 1 cut(s) 976
AjnI CCWGG 2 cut(s) 835, 1510
AluBI AGCT 5 cut(s) 374, 911, 1062, 1280, 1496
AluI AGCT 5 cut(s) 374, 911, 1062, 1280, 1496
Alw21I GWGCWC 3 cut(s) 12, 969, 1064
AlwI GGATC 1 cut(s) 580
AlwNI CAGNNNCTG 1 cut(s) 212
AoxI GGCC 2 cut(s) 714, 806
ApeKI GCWGC 7 cut(s) 374, 455, 563, 825, 1019, 1232, 1496
ApoI RAATTY 4 cut(s) 202, 709, 951, 1003
Asp700I GAANNNNTTC 2 cut(s) 808, 921
AspLEI GCGC 1 cut(s) 1408
AsuC2I CCSGG 1 cut(s) 175
AsuHPI GGTGA 2 cut(s) 35, 159
AsuNHI GCTAGC 1 cut(s) 194
BanI GGYRCC 1 cut(s) 693
BanII GRGCYC 1 cut(s) 1064
BauI CACGAG 1 cut(s) 1856
BbsI GAAGAC 1 cut(s) 978
Bbv12I GWGCWC 3 cut(s) 12, 969, 1064
BbvI GCAGC 7 cut(s) 361, 467, 550, 837, 1031, 1219, 1483
BccI CCATC 5 cut(s) 14, 1345, 1580, 1732, 1779
BceAI ACGGC 2 cut(s) 207, 1891
BciT130I CCWGG 2 cut(s) 837, 1512
BciVI GTATCC 1 cut(s) 391
BclI TGATCA 4 cut(s) 492, 987, 1204, 1525
BcnI CCSGG 1 cut(s) 175
BcuI ACTAGT 1 cut(s) 928
BfmI CTRYAG 1 cut(s) 1722
BfoI RGCGCY 1 cut(s) 1409
BfrI CTTAAG 2 cut(s) 323, 1961
BfuAI ACCTGC 1 cut(s) 178
BfuI GTATCC 1 cut(s) 391
BglI GCCNNNNNGGC 2 cut(s) 566, 659
Bme1390I CCNGG 3 cut(s) 175, 837, 1512
BmgBI CACGTC 1 cut(s) 976
BmiI GGNNCC 2 cut(s) 136, 695
BmrFI CCNGG 3 cut(s) 175, 837, 1512
BmrI ACTGGG 2 cut(s) 222, 1817
BmsI GCATC 4 cut(s) 252, 1138, 1300, 1736
BmtI GCTAGC 1 cut(s) 198
BmuI ACTGGG 2 cut(s) 222, 1817
BpiI GAAGAC 1 cut(s) 978
BpuEI CTTGAG 2 cut(s) 1362, 1873
BpuMI CCSGG 1 cut(s) 175
Bsa29I ATCGAT 1 cut(s) 623
BsaAI YACGTR 1 cut(s) 758
BsaJI CCNNGG 3 cut(s) 138, 189, 1907
BsaWI WCCGGW 1 cut(s) 1532
Bse1I ACTGG 4 cut(s) 50, 78, 217, 1823
Bse3DI GCAATG 1 cut(s) 1623
BseBI CCWGG 2 cut(s) 837, 1512
BseCI ATCGAT 1 cut(s) 623
BseDI CCNNGG 3 cut(s) 138, 189, 1907
BseGI GGATG 4 cut(s) 1207, 1437, 1635, 1727
BseMI GCAATG 1 cut(s) 1623
BseMII CTCAG 3 cut(s) 401, 1062, 1352
BseNI ACTGG 4 cut(s) 50, 78, 217, 1823
BseXI GCAGC 7 cut(s) 361, 467, 550, 837, 1031, 1219, 1483
BsgI GTGCAG 1 cut(s) 738
Bsh1236I CGCG 1 cut(s) 1648
BshFI GGCC 2 cut(s) 716, 808
BshNI GGYRCC 1 cut(s) 693
BshVI ATCGAT 1 cut(s) 623
BsiHKAI GWGCWC 3 cut(s) 12, 969, 1064
BsiSI CCGG 2 cut(s) 174, 1533
BslFI GGGAC 2 cut(s) 687, 1805
BsmFI GGGAC 2 cut(s) 687, 1805
BsnI GGCC 2 cut(s) 716, 808
Bsp1286I GDGCHC 3 cut(s) 12, 969, 1064
Bsp1407I TGTACA 1 cut(s) 1948
Bsp143I GATC 6 cut(s) 492, 572, 987, 1173, 1204, 1525
Bsp19I CCATGG 1 cut(s) 138
BspACI CCGC 9 cut(s) 198, 233, 377, 561, 566, 633, 1049, 1648, 1752
BspANI GGCC 2 cut(s) 716, 808
BspCNI CTCAG 3 cut(s) 402, 1063, 1351
BspDI ATCGAT 1 cut(s) 623
BspFNI CGCG 1 cut(s) 1648
BspLI GGNNCC 2 cut(s) 136, 695
BspMI ACCTGC 1 cut(s) 178
BspOI GCTAGC 1 cut(s) 198
BspPI GGATC 1 cut(s) 580
BspQI GCTCTTC 1 cut(s) 1287
BspT107I GGYRCC 1 cut(s) 693
BspTI CTTAAG 2 cut(s) 323, 1961
BsrDI GCAATG 1 cut(s) 1623
BsrGI TGTACA 1 cut(s) 1948
BsrI ACTGG 4 cut(s) 50, 78, 217, 1823
BssECI CCNNGG 3 cut(s) 138, 189, 1907
BssMI GATC 6 cut(s) 492, 572, 987, 1173, 1204, 1525
BssSI CACGAG 1 cut(s) 1856
BssT1I CCWWGG 1 cut(s) 138
Bst2BI CACGAG 1 cut(s) 1856
Bst2UI CCWGG 2 cut(s) 837, 1512
Bst4CI ACNGT 4 cut(s) 442, 678, 723, 1483
Bst6I CTCTTC 5 cut(s) 51, 79, 1287, 1612, 1879
BstAFI CTTAAG 2 cut(s) 323, 1961
BstAUI TGTACA 1 cut(s) 1948
BstBAI YACGTR 1 cut(s) 758
BstC8I GCNNGC 6 cut(s) 98, 102, 196, 418, 889, 1457
BstDEI CTNAG 4 cut(s) 410, 1071, 1338, 1392
BstDSI CCRYGG 3 cut(s) 138, 189, 1907
BstF5I GGATG 4 cut(s) 1207, 1437, 1635, 1727
BstFNI CGCG 1 cut(s) 1648
BstH2I RGCGCY 1 cut(s) 1409
BstHHI GCGC 1 cut(s) 1408
BstKTI GATC 6 cut(s) 495, 575, 990, 1176, 1207, 1528
BstMBI GATC 6 cut(s) 492, 572, 987, 1173, 1204, 1525
BstMWI GCNNNNNNNGC 8 cut(s) 292, 461, 566, 659, 917, 1241, 1405, 1835
BstNI CCWGG 2 cut(s) 837, 1512
BstNSI RCATGY 5 cut(s) 100, 104, 333, 1870, 1922
BstSCI CCNGG 3 cut(s) 173, 835, 1510
BstSFI CTRYAG 1 cut(s) 1722
BstUI CGCG 1 cut(s) 1648
BstV1I GCAGC 7 cut(s) 361, 467, 550, 837, 1031, 1219, 1483
BstV2I GAAGAC 1 cut(s) 978
BstX2I RGATCY 1 cut(s) 572
BstXI CCANNNNNNTGG 3 cut(s) 20, 843, 1738
BstYI RGATCY 1 cut(s) 572
Bsu15I ATCGAT 1 cut(s) 623
BsuI GTATCC 1 cut(s) 391
BsuRI GGCC 2 cut(s) 716, 808
BsuTUI ATCGAT 1 cut(s) 623
BtgI CCRYGG 3 cut(s) 138, 189, 1907
BtrI CACGTC 1 cut(s) 976
BtsCI GGATG 4 cut(s) 1207, 1437, 1635, 1727
BtsI GCAGTG 3 cut(s) 521, 543, 1440
BtsIMutI CAGTG 3 cut(s) 521, 543, 1440
BveI ACCTGC 1 cut(s) 178
Cac8I GCNNGC 6 cut(s) 98, 102, 196, 418, 889, 1457
CaiI CAGNNNCTG 1 cut(s) 212
CfoI GCGC 1 cut(s) 1408
ClaI ATCGAT 1 cut(s) 623
CseI GACGC 2 cut(s) 1025, 1403
CsiI ACCWGGT 1 cut(s) 1510
Csp6I GTAC 5 cut(s) 700, 759, 1651, 1949, 1958
CviQI GTAC 5 cut(s) 700, 759, 1651, 1949, 1958
DdeI CTNAG 4 cut(s) 410, 1071, 1338, 1392
DpnI GATC 6 cut(s) 494, 574, 989, 1175, 1206, 1527
DpnII GATC 6 cut(s) 492, 572, 987, 1173, 1204, 1525
DraI TTTAAA 1 cut(s) 957
DraIII CACNNNGTG 1 cut(s) 93
Eam1104I CTCTTC 5 cut(s) 51, 79, 1287, 1612, 1879
EarI CTCTTC 5 cut(s) 51, 79, 1287, 1612, 1879
Ecl136II GAGCTC 1 cut(s) 1062
Eco130I CCWWGG 1 cut(s) 138
Eco147I AGGCCT 1 cut(s) 808
Eco24I GRGCYC 1 cut(s) 1064
Eco53kI GAGCTC 1 cut(s) 1062
Eco57I CTGAAG 1 cut(s) 795
EcoICRI GAGCTC 1 cut(s) 1062
EcoRI GAATTC 1 cut(s) 202
EcoRII CCWGG 2 cut(s) 835, 1510
EcoT14I CCWWGG 1 cut(s) 138
EcoT22I ATGCAT 2 cut(s) 102, 1293
EcoT38I GRGCYC 1 cut(s) 1064
ErhI CCWWGG 1 cut(s) 138
FalI AAGNNNNNCTT 4 cut(s) 306, 338, 1529, 1561
FaqI GGGAC 2 cut(s) 687, 1805
FauI CCCGC 1 cut(s) 191
FauNDI CATATG 2 cut(s) 1195, 1227
FbaI TGATCA 4 cut(s) 492, 987, 1204, 1525
FblI GTMKAC 1 cut(s) 850
FokI GGATG 4 cut(s) 1214, 1444, 1622, 1714
FriOI GRGCYC 1 cut(s) 1064
GlaI GCGC 1 cut(s) 1407
HaeII RGCGCY 1 cut(s) 1409
HaeIII GGCC 2 cut(s) 716, 808
HapII CCGG 2 cut(s) 174, 1533
HgaI GACGC 2 cut(s) 1025, 1403
HhaI GCGC 1 cut(s) 1408
Hin6I GCGC 1 cut(s) 1406
HinP1I GCGC 1 cut(s) 1406
HinfI GANTC 9 cut(s) 29, 178, 260, 384, 625, 1261, 1424, 1701, 1778
HpaII CCGG 2 cut(s) 174, 1533
HphI GGTGA 2 cut(s) 35, 159
Hpy166II GTNNAC 1 cut(s) 851
Hpy188I TCNGA 4 cut(s) 411, 682, 1795, 1864
Hpy188III TCNNGA 9 cut(s) 132, 388, 426, 578, 589, 1070, 1295, 1379, 1782
Hpy8I GTNNAC 1 cut(s) 851
HpyAV CCTTC 7 cut(s) 455, 798, 819, 932, 1008, 1765, 1925
HpyCH4III ACNGT 4 cut(s) 442, 678, 723, 1483
HpyCH4IV ACGT 7 cut(s) 450, 469, 507, 531, 757, 937, 975
HpyF10VI GCNNNNNNNGC 8 cut(s) 292, 461, 566, 659, 917, 1241, 1405, 1835
HpyF3I CTNAG 4 cut(s) 410, 1071, 1338, 1392
HpySE526I ACGT 7 cut(s) 450, 469, 507, 531, 757, 937, 975
HspAI GCGC 1 cut(s) 1406
Ksp22I TGATCA 4 cut(s) 492, 987, 1204, 1525
Kzo9I GATC 6 cut(s) 492, 572, 987, 1173, 1204, 1525
LguI GCTCTTC 1 cut(s) 1287
LmnI GCTCC 3 cut(s) 15, 461, 1396
Lsp1109I GCAGC 7 cut(s) 361, 467, 550, 837, 1031, 1219, 1483
LweI GCATC 4 cut(s) 252, 1138, 1300, 1736
MabI ACCWGGT 1 cut(s) 1510
MaeII ACGT 7 cut(s) 450, 469, 507, 531, 757, 937, 975
MaeIII GTNAC 3 cut(s) 23, 1189, 1211
MalI GATC 6 cut(s) 494, 574, 989, 1175, 1206, 1527
MboI GATC 6 cut(s) 492, 572, 987, 1173, 1204, 1525
MboII GAAGA 8 cut(s) 38, 66, 326, 959, 983, 1274, 1599, 1866
MflI RGATCY 1 cut(s) 572
MhlI GDGCHC 3 cut(s) 12, 969, 1064
MlyI GAGTC 2 cut(s) 23, 1695
MmeI TCCRAC 2 cut(s) 958, 1887
Mph1103I ATGCAT 2 cut(s) 102, 1293
MroXI GAANNNNTTC 2 cut(s) 808, 921
MseI TTAA 7 cut(s) 185, 324, 902, 956, 1149, 1550, 1962
MslI CAYNNNNRTG 3 cut(s) 18, 1134, 1736
MspA1I CMGCKG 3 cut(s) 233, 563, 1496
MspCI CTTAAG 2 cut(s) 323, 1961
MspI CCGG 2 cut(s) 174, 1533
MspR9I CCNGG 3 cut(s) 175, 837, 1512
MvaI CCWGG 2 cut(s) 837, 1512
MvnI CGCG 1 cut(s) 1648
MwoI GCNNNNNNNGC 8 cut(s) 292, 461, 566, 659, 917, 1241, 1405, 1835
NciI CCSGG 1 cut(s) 175
NcoI CCATGG 1 cut(s) 138
NdeI CATATG 2 cut(s) 1195, 1227
NdeII GATC 6 cut(s) 492, 572, 987, 1173, 1204, 1525
NheI GCTAGC 1 cut(s) 194
NlaIV GGNNCC 2 cut(s) 136, 695
NmuCI GTSAC 2 cut(s) 23, 1189
NsiI ATGCAT 2 cut(s) 102, 1293
NspI RCATGY 5 cut(s) 100, 104, 333, 1870, 1922
PaeI GCATGC 2 cut(s) 100, 104
PaqCI CACCTGC 1 cut(s) 178
PceI AGGCCT 1 cut(s) 808
PciI ACATGT 2 cut(s) 329, 1866
PciSI GCTCTTC 1 cut(s) 1287
PcsI WCGNNNNNNNCGW 1 cut(s) 1659
PdmI GAANNNNTTC 2 cut(s) 808, 921
PfeI GAWTC 7 cut(s) 178, 260, 384, 625, 1261, 1424, 1778
PflFI GACNNNGTC 1 cut(s) 584
PfoI TCCNGGA 1 cut(s) 835
PleI GAGTC 2 cut(s) 23, 1695
PpsI GAGTC 2 cut(s) 23, 1695
Ppu21I YACGTR 1 cut(s) 758
PscI ACATGT 2 cut(s) 329, 1866
Psp124BI GAGCTC 1 cut(s) 1064
Psp6I CCWGG 2 cut(s) 835, 1510
PspGI CCWGG 2 cut(s) 835, 1510
PspN4I GGNNCC 2 cut(s) 136, 695
PsrI GAACNNNNNNTAC 2 cut(s) 127, 159
PstNI CAGNNNCTG 1 cut(s) 212
PsuI RGATCY 1 cut(s) 572
PsyI GACNNNGTC 1 cut(s) 584
PvuII CAGCTG 1 cut(s) 1496
RsaI GTAC 5 cut(s) 701, 760, 1652, 1950, 1959
RsaNI GTAC 5 cut(s) 700, 759, 1651, 1949, 1958
RseI CAYNNNNRTG 3 cut(s) 18, 1134, 1736
SacI GAGCTC 1 cut(s) 1064
SapI GCTCTTC 1 cut(s) 1287
SaqAI TTAA 7 cut(s) 185, 324, 902, 956, 1149, 1550, 1962
Sau3AI GATC 6 cut(s) 492, 572, 987, 1173, 1204, 1525
SchI GAGTC 2 cut(s) 23, 1695
ScrFI CCNGG 3 cut(s) 175, 837, 1512
SduI GDGCHC 3 cut(s) 12, 969, 1064
SexAI ACCWGGT 1 cut(s) 1510
SfaNI GCATC 4 cut(s) 252, 1138, 1300, 1736
SfcI CTRYAG 1 cut(s) 1722
SmiMI CAYNNNNRTG 3 cut(s) 18, 1134, 1736
SmlI CTYRAG 4 cut(s) 323, 1377, 1888, 1961
SmoI CTYRAG 4 cut(s) 323, 1377, 1888, 1961
SpeI ACTAGT 1 cut(s) 928
SphI GCATGC 2 cut(s) 100, 104
SseBI AGGCCT 1 cut(s) 808
SsiI CCGC 9 cut(s) 198, 233, 377, 561, 566, 633, 1049, 1648, 1752
SstI GAGCTC 1 cut(s) 1064
StuI AGGCCT 1 cut(s) 808
StyD4I CCNGG 3 cut(s) 173, 835, 1510
StyI CCWWGG 1 cut(s) 138
TaaI ACNGT 4 cut(s) 442, 678, 723, 1483
TaiI ACGT 7 cut(s) 453, 472, 510, 534, 760, 940, 978
TaqI TCGA 6 cut(s) 246, 623, 628, 1102, 1172, 1294
TatI WGTACW 2 cut(s) 1948, 1957
TauI GCSGC 3 cut(s) 563, 569, 1052
TfiI GAWTC 7 cut(s) 178, 260, 384, 625, 1261, 1424, 1778
Tru1I TTAA 7 cut(s) 185, 324, 902, 956, 1149, 1550, 1962
Tru9I TTAA 7 cut(s) 185, 324, 902, 956, 1149, 1550, 1962
TscAI CASTG 3 cut(s) 528, 550, 1447
TseFI GTSAC 2 cut(s) 23, 1189
TseI GCWGC 7 cut(s) 374, 455, 563, 825, 1019, 1232, 1496
Tsp45I GTSAC 2 cut(s) 23, 1189
TspRI CASTG 3 cut(s) 528, 550, 1447
Tth111I GACNNNGTC 1 cut(s) 584
Vha464I CTTAAG 2 cut(s) 323, 1961
XapI RAATTY 4 cut(s) 202, 709, 951, 1003
XbaI TCTAGA 2 cut(s) 425, 577
XceI RCATGY 5 cut(s) 100, 104, 333, 1870, 1922
XmiI GTMKAC 1 cut(s) 850
XmnI GAANNNNTTC 2 cut(s) 808, 921
Zsp2I ATGCAT 2 cut(s) 102, 1293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.