Rroxscaffold_1G00039220

Ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000157
Physical Location & Seq
Forward (+)
56794981 .. 56801349
6369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00039220.1

Sequence Viewer

Length: 1869 bp
ATGTCTATCCCACAAGAACAAAATGTATCACCTTCCCGGATTCAATTAACCACAGCTACCCGCGATTCAGCGACTGGGATAGACAACCAATCCGCAGACATTGCCATCGACATTTCAGCAGATCATTCGGCTCAACTTGCTAACAGACCTTCGTCTTATTTACTAGAACATCCAAACAGAGAAGAATCCCGTAAGACACGTGTTGCCCTCTATAAGTATGCACTAAAAGGCGATTGGGAAGCCGCTGAACCAATCTTGAAAGAGAATAGCAAACTTCTGCGAGCAAGCATATCTAGAGGATATGAAACAGTCCTTCACGTTGCAGCAGGAACAAACCATATTCACTTTGTGGAGAAACTGGTCGACATGATGAAAGTAGAAGACTTGGCACTGCAGGACGAAAATGAAAACACTGCCTTATGCATTGCCGCTGCGGCTGGATCTCTAGAAATTGTCCATATTTTGATACAAAAGAACGAGTCTTTGCTAACAATTAGAGGAGGTAAAGATATGGCACCAGCCTACATGGCTGCTCTGTTAGGACAGTCCGAAATGGCATGGTACCTGTACCGCGAAAGTAAAAAGATGTTTGAGGAAGCCGACCAAGAAAACTTGTTCTTTTCTTGCATCAACAATTGTTTGTACGATTTGGCCTTTCAGTTGCTAGATGCTAATAAAGCATTAGCGAAGGCACGTACTAGTTCGCAGAACAATAAAACGGCCTTGCATATCCTGGCTCGGAGACCTTCAGCATTTGGTGGCAGCCAAAGTCCACCAATGTGGCCTAGACTCATTAACTCATTCCTCCCAGGTTTGAAGTTCGCACGCAAAAGAAAGTTGAGAAAAGCAAATGGAGCCCTTCAACTAGTCCACCGCCTCTGGGAAGAAGTCTTGAGAAATGATGACCATGATGACGTGATGGGGCTGATCAAATATCCTTCAAATTTATTATTTGACGCAGCAGAATTAGGAAATTATGAGTTTTTGGCAGCACTTCTGAGCTCTTATCCTGAGTTAGTATGGGAAACTAATGAAGAAAATCGAACCATAATCCATGTCGCAGTTCTGCATCGTCACGCAAGTATCTTCAACCTAGTGCATGAAATAGGCTCAATCAAAGATGTCATCGTGACATATGAAGATGATCAGGGTAACAACATTGTGCATATGGCTGCAAAATTAGCACCTCAAAATCAATTGAATCTCGTGTCAGGCGTGGCTCTTCAAATGCATCGAGAGTTGGTATGGTTTGAGGAAGTCAAAAAGATTGTCCAACCTCAATACATCGAGATGGAAAACGACAAAGGAAAAACACCTCAAGAATTATTTACTGAAGAGCATACGGTGTTAATGCGTGAAGGAGAAAAATGGATGAAGGACACTGCAAGTTCATGCATGCTCGTTGCAACTATTATTGCAACCGTTGTGTTCTCAGCTACATTTAGCATACCGGGTGGCTCCGATGATCATACTGGAAAGCCAAATCTTTTGAAACAGAAAGCCTTTCTACACTTTACCATAGCTGACGAAGTAGCACTCTTTTCCTCTTCAACTGCAATGTTGATGTTCTTGTTCATCCTTACCTCGCGCTATGCAGAAAATGATTTCCGCAAGTCCTTACCCTTGAAGTTGATGGTAGGACTCACATGCCTCTTCATATCTATAGCATCGATGATGATGGCTTTCAGCACCGCCTTCTATCTATCTTGTCAATACGAATCAAGATGGATTTCAAATTTTACATTTTTATTTGCATTTGTCCCAGTTGCTTTGTTTGCGTTTATGCAATTCCCTCTTGTGTCCGACATGTCCTCATCTATATTTTGCTCAAGTCTACTATTTCAGCCATGGAAACACATGCTCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

622

Amino Acids

70.18

Weight (kDa)

5.98

Isoelectric Point (pI)

45.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 95 - 159 1.4e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 105 - 191 5.5e-09 Ankyrin repeats (3 copies)
PGG PF13962 455 - 568 1.9e-27 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54070 AT5G35830
fragaria_vesca FvH4_3g25070 FvH4_3g25080 FvH4_3g25081 FvH4_3g25081
malus_domestica MD03G1027500.v1.1 MD03G1146300.v1.1 MD03G1146800.v1.1 MD03G1146900.v1.1 MD03G1147100.v1.1 MD11G1165600.v1.1
prunus_persica Prupe.2G015800_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G016300_v2.0.a1 Prupe.2G016500_v2.0.a1 Prupe.6G131100_v2.0.a1 Prupe.6G131400_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1
pyrus_communis pycom03g10090 pycom03g10110 pycom11g13740 pycom11g13770
rosa_chinensis RchiOBHm_Chr1g0316371 RchiOBHm_Chr1g0316381 RchiOBHm_Chr1g0316401 RchiOBHm_Chr1g0316411 RchiOBHm_Chr1g0316571 RchiOBHm_Chr1g0316601 RchiOBHm_Chr5g0039341 RchiOBHm_Chr5g0041371 RchiOBHm_Chr5g0041401 RchiOBHm_Chr5g0041431 RchiOBHm_Chr5g0041471 RchiOBHm_Chr5g0045281 RchiOBHm_Chr5g0045291 RchiOBHm_Chr5g0045311 RchiOBHm_Chr5g0045371 RchiOBHm_Chr5g0045421 RchiOBHm_Chr5g0045431 RchiOBHm_Chr5g0045501 RchiOBHm_Chr5g0045551 RchiOBHm_Chr5g0045771 RchiOBHm_Chr5g0045811
rosa_laevigata RLG00000034057 RLG00000034058
rosa_multiflora Rmu_co8352751.1_g000001 Rmu_co8397575.1_g000001 Rmu_sc0000415.1_g000006 Rmu_sc0000493.1_g000057 Rmu_sc0000504.1_g000003 Rmu_sc0000594.1_g000019 Rmu_sc0000594.1_g000032 Rmu_sc0000594.1_g000066 Rmu_sc0001047.1_g000003 Rmu_sc0001047.1_g000011 Rmu_sc0001556.1_g000023 Rmu_sc0002584.1_g000003 Rmu_sc0002584.1_g000039 Rmu_sc0002584.1_g000070 Rmu_sc0002667.1_g000015 Rmu_sc0003677.1_g000003 Rmu_sc0003743.1_g000017 Rmu_sc0004520.1_g000004 Rmu_sc0004520.1_g000013 Rmu_sc0004520.1_g000027 Rmu_sc0005539.1_g000014 Rmu_sc0005539.1_g000032 Rmu_sc0006018.1_g000006 Rmu_sc0006737.1_g000020 Rmu_sc0007485.1_g000002 Rmu_sc0008729.1_g000002 Rmu_sc0008729.1_g000003 Rmu_sc0038822.1_g000001 Rmu_ssc0000398.1_g000025
rosa_roxburghii Rroxscaffold_1G00035090 Rroxscaffold_1G00035630 Rroxscaffold_1G00035640 Rroxscaffold_1G00035660 Rroxscaffold_1G00035680 Rroxscaffold_1G00035730 Rroxscaffold_1G00035750 Rroxscaffold_1G00035770 Rroxscaffold_1G00039150 Rroxscaffold_1G00039190 Rroxscaffold_1G00039220 Rroxscaffold_1G00039260 Rroxscaffold_4G00328910 Rroxscaffold_4G00328950 Rroxscaffold_4G00328960 Rroxscaffold_4G00330890
rosa_rugosa Rorug01G0009800 Rorug01G0010000.1 Rorug01G0010200 Rorug01G0012100 Rorug01G0023500 Rorug01G0023600 Rorug01G0023700 Rorug01G0024700 Rorug05G0192000 Rorug05G0192200 Rorug05G0222400 Rorug05G0222500 Rorug05G0222600.1 Rorug05G0222700.1 Rorug05G0222800.1 Rorug05G0222900.1 Rorug05G0223000.1 Rorug05G0223100.1 Rorug05G0223200 Rorug05G0223600 Rorug05G0226800 Rorug05G0226900
rosa_samantha Rh1CG019600 Rh1DG015000 Rh1DG015300 Rh1DG016900 Rh5CG315200 Rh5CG338700 Rh5DG277100 Rh5DG291500 Rh5DG292100 Rh5DG292200 Rh5DG292400 Rh5DG292600 Rh5DG322100 Rh5DG322200 Rh5DG323100 Rh5DG323300 Rh5DG326700 Rh5DG326900 Rh5DG327000
rosa_wichuraiana Rw0G021580 Rw1G001320 Rw1G001420 Rw1G002820 Rw1G002840 Rw1G002870 Rw5G024800 Rw5G026160 Rw5G026170 Rw5G026180 Rw5G026210 Rw5G026230 Rw5G028170 Rw5G028190 Rw5G028210 Rw5G028230 Rw5G028240 Rw5G028260 Rw5G028280 Rw5G028370 Rw5G028380 Rw5G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 561
AccB1I GGYRCC 2 cut(s) 514, 561
AccI GTMKAC 2 cut(s) 363, 1834
AccII CGCG 3 cut(s) 63, 573, 1588
AciI CCGC 9 cut(s) 61, 93, 243, 429, 434, 571, 874, 1609, 1692
AclWI GGATC 1 cut(s) 448
AcsI RAATTY 2 cut(s) 943, 1735
AcuI CTGAAG 2 cut(s) 732, 1353
AcvI CACGTG 1 cut(s) 200
AdeI CACNNNGTG 1 cut(s) 349
AfaI GTAC 4 cut(s) 563, 569, 644, 697
AfiI CCNNNNNNNGG 1 cut(s) 880
AflIII ACRYGT 3 cut(s) 197, 199, 1806
AhlI ACTAGT 2 cut(s) 698, 865
AjiI CACGTC 1 cut(s) 916
AjnI CCWGG 2 cut(s) 732, 808
AleI CACNNNNGTG 1 cut(s) 778
AluBI AGCT 4 cut(s) 56, 1002, 1436, 1523
AluI AGCT 4 cut(s) 56, 1002, 1436, 1523
Alw21I GWGCWC 1 cut(s) 1004
Alw26I GTCTC 1 cut(s) 736
AlwI GGATC 1 cut(s) 448
AlwNI CAGNNNCTG 1 cut(s) 74
AoxI GGCC 3 cut(s) 651, 720, 782
ApeKI GCWGC 7 cut(s) 323, 431, 530, 762, 959, 989, 1172
ApoI RAATTY 2 cut(s) 943, 1735
ArsI GACNNNNNNTTYG 2 cut(s) 1254, 1286
Asp718I GGTACC 1 cut(s) 561
AspLEI GCGC 1 cut(s) 1590
AsuC2I CCSGG 2 cut(s) 37, 1452
AsuHPI GGTGA 1 cut(s) 21
BaeI ACNNNNGTAYC 2 cut(s) 1066, 1099
BanI GGYRCC 2 cut(s) 514, 561
BanII GRGCYC 2 cut(s) 859, 1004
BauI CACGAG 1 cut(s) 1205
BbrPI CACGTG 1 cut(s) 200
BbsI GAAGAC 1 cut(s) 387
Bbv12I GWGCWC 1 cut(s) 1004
BbvI GCAGC 7 cut(s) 335, 418, 517, 774, 971, 1001, 1159
BccI CCATC 6 cut(s) 113, 913, 1285, 1627, 1672, 1719
BceAI ACGGC 1 cut(s) 735
BcgI CGANNNNNNTGC 2 cut(s) 108, 142
BciT130I CCWGG 2 cut(s) 734, 810
BclI TGATCA 3 cut(s) 927, 1144, 1465
BcnI CCSGG 2 cut(s) 37, 1452
BcoDI GTCTC 1 cut(s) 736
BcuI ACTAGT 2 cut(s) 698, 865
BfaI CTAG 8 cut(s) 164, 294, 446, 665, 699, 786, 866, 1094
BfmI CTRYAG 2 cut(s) 392, 1662
BglI GCCNNNNNGGC 2 cut(s) 434, 527
Bme1390I CCNGG 4 cut(s) 37, 734, 810, 1452
BmgBI CACGTC 1 cut(s) 916
BmiI GGNNCC 4 cut(s) 516, 563, 856, 1459
BmrFI CCNGG 4 cut(s) 37, 734, 810, 1452
BmrI ACTGGG 2 cut(s) 84, 1757
BmsI GCATC 5 cut(s) 636, 658, 1078, 1240, 1676
BmuI ACTGGG 2 cut(s) 84, 1757
BoxI GACNNNNGTC 1 cut(s) 151
BpiI GAAGAC 1 cut(s) 387
BpuEI CTTGAG 3 cut(s) 913, 1302, 1813
BpuMI CCSGG 2 cut(s) 37, 1452
Bsa29I ATCGAT 1 cut(s) 1670
BsaAI YACGTR 2 cut(s) 200, 695
BsaI GGTCTC 1 cut(s) 736
BsaJI CCNNGG 2 cut(s) 808, 1847
Bsc4I CCNNNNNNNGG 1 cut(s) 880
Bse1I ACTGG 4 cut(s) 79, 363, 1477, 1763
Bse3DI GCAATG 3 cut(s) 99, 423, 1563
BseBI CCWGG 2 cut(s) 734, 810
BseCI ATCGAT 1 cut(s) 1670
BseDI CCNNGG 2 cut(s) 808, 1847
BseGI GGATG 3 cut(s) 169, 1377, 1575
BseLI CCNNNNNNNGG 1 cut(s) 880
BseMI GCAATG 3 cut(s) 99, 423, 1563
BseMII CTCAG 3 cut(s) 989, 1002, 1446
BseNI ACTGG 4 cut(s) 79, 363, 1477, 1763
BseRI GAGGAG 1 cut(s) 513
BseXI GCAGC 7 cut(s) 335, 418, 517, 774, 971, 1001, 1159
Bsh1236I CGCG 3 cut(s) 63, 573, 1588
BshFI GGCC 3 cut(s) 653, 722, 784
BshNI GGYRCC 2 cut(s) 514, 561
BshVI ATCGAT 1 cut(s) 1670
BsiHKAI GWGCWC 1 cut(s) 1004
BsiSI CCGG 2 cut(s) 37, 1451
BslFI GGGAC 1 cut(s) 1745
BslI CCNNNNNNNGG 1 cut(s) 880
BsmAI GTCTC 1 cut(s) 736
BsmFI GGGAC 1 cut(s) 1745
BsnI GGCC 3 cut(s) 653, 722, 784
Bso31I GGTCTC 1 cut(s) 736
Bsp1286I GDGCHC 2 cut(s) 859, 1004
Bsp143I GATC 5 cut(s) 121, 440, 927, 1144, 1465
Bsp19I CCATGG 1 cut(s) 1847
BspACI CCGC 9 cut(s) 61, 93, 243, 429, 434, 571, 874, 1609, 1692
BspANI GGCC 3 cut(s) 653, 722, 784
BspCNI CTCAG 3 cut(s) 990, 1003, 1445
BspDI ATCGAT 1 cut(s) 1670
BspFNI CGCG 3 cut(s) 63, 573, 1588
BspLI GGNNCC 4 cut(s) 516, 563, 856, 1459
BspMAI CTGCAG 1 cut(s) 396
BspPI GGATC 1 cut(s) 448
BspQI GCTCTTC 2 cut(s) 1227, 1329
BspT107I GGYRCC 2 cut(s) 514, 561
BspTNI GGTCTC 1 cut(s) 736
BsrDI GCAATG 3 cut(s) 99, 423, 1563
BsrI ACTGG 4 cut(s) 79, 363, 1477, 1763
BssECI CCNNGG 2 cut(s) 808, 1847
BssMI GATC 5 cut(s) 121, 440, 927, 1144, 1465
BssSI CACGAG 1 cut(s) 1205
BssT1I CCWWGG 1 cut(s) 1847
Bst2BI CACGAG 1 cut(s) 1205
Bst2UI CCWGG 2 cut(s) 734, 810
Bst4CI ACNGT 4 cut(s) 310, 546, 1345, 1423
Bst6I CTCTTC 4 cut(s) 1227, 1329, 1552, 1658
BstAPI GCANNNNNTGC 1 cut(s) 101
BstBAI YACGTR 2 cut(s) 200, 695
BstC8I GCNNGC 4 cut(s) 282, 286, 826, 1397
BstDEI CTNAG 3 cut(s) 998, 1011, 1432
BstDSI CCRYGG 1 cut(s) 1847
BstF5I GGATG 3 cut(s) 169, 1377, 1575
BstFNI CGCG 3 cut(s) 63, 573, 1588
BstHHI GCGC 1 cut(s) 1590
BstKTI GATC 5 cut(s) 124, 443, 930, 1147, 1468
BstMAI GTCTC 1 cut(s) 736
BstMBI GATC 5 cut(s) 121, 440, 927, 1144, 1465
BstMWI GCNNNNNNNGC 8 cut(s) 101, 137, 434, 527, 677, 854, 1181, 1775
BstNI CCWGG 2 cut(s) 734, 810
BstNSI RCATGY 4 cut(s) 1399, 1650, 1810, 1861
BstPAI GACNNNNGTC 1 cut(s) 151
BstSCI CCNGG 4 cut(s) 35, 732, 808, 1450
BstSFI CTRYAG 2 cut(s) 392, 1662
BstUI CGCG 3 cut(s) 63, 573, 1588
BstV1I GCAGC 7 cut(s) 335, 418, 517, 774, 971, 1001, 1159
BstV2I GAAGAC 1 cut(s) 387
BstX2I RGATCY 1 cut(s) 440
BstXI CCANNNNNNTGG 1 cut(s) 780
BstYI RGATCY 1 cut(s) 440
Bsu15I ATCGAT 1 cut(s) 1670
BsuRI GGCC 3 cut(s) 653, 722, 784
BsuTUI ATCGAT 1 cut(s) 1670
BtgI CCRYGG 1 cut(s) 1847
BtrI CACGTC 1 cut(s) 916
BtsCI GGATG 3 cut(s) 169, 1377, 1575
BtsI GCAGTG 3 cut(s) 389, 411, 1380
BtsIMutI CAGTG 3 cut(s) 389, 411, 1380
Cac8I GCNNGC 4 cut(s) 282, 286, 826, 1397
CaiI CAGNNNCTG 1 cut(s) 74
CfoI GCGC 1 cut(s) 1590
ClaI ATCGAT 1 cut(s) 1670
CseI GACGC 1 cut(s) 965
Csp6I GTAC 4 cut(s) 562, 568, 643, 696
CviQI GTAC 4 cut(s) 562, 568, 643, 696
DdeI CTNAG 3 cut(s) 998, 1011, 1432
DpnI GATC 5 cut(s) 123, 442, 929, 1146, 1467
DpnII GATC 5 cut(s) 121, 440, 927, 1144, 1465
DraIII CACNNNGTG 1 cut(s) 349
Eam1104I CTCTTC 4 cut(s) 1227, 1329, 1552, 1658
EarI CTCTTC 4 cut(s) 1227, 1329, 1552, 1658
Ecl136II GAGCTC 1 cut(s) 1002
Eco130I CCWWGG 1 cut(s) 1847
Eco24I GRGCYC 2 cut(s) 859, 1004
Eco31I GGTCTC 1 cut(s) 736
Eco53kI GAGCTC 1 cut(s) 1002
Eco57I CTGAAG 2 cut(s) 732, 1353
Eco72I CACGTG 1 cut(s) 200
EcoICRI GAGCTC 1 cut(s) 1002
EcoRII CCWGG 2 cut(s) 732, 808
EcoT14I CCWWGG 1 cut(s) 1847
EcoT22I ATGCAT 3 cut(s) 425, 1233, 1397
EcoT38I GRGCYC 2 cut(s) 859, 1004
ErhI CCWWGG 1 cut(s) 1847
FaqI GGGAC 1 cut(s) 1745
FauI CCCGC 1 cut(s) 68
FauNDI CATATG 2 cut(s) 1135, 1167
FbaI TGATCA 3 cut(s) 927, 1144, 1465
FblI GTMKAC 2 cut(s) 363, 1834
FokI GGATG 3 cut(s) 156, 1384, 1562
FriOI GRGCYC 2 cut(s) 859, 1004
FspBI CTAG 8 cut(s) 164, 294, 446, 665, 699, 786, 866, 1094
GlaI GCGC 1 cut(s) 1589
HaeIII GGCC 3 cut(s) 653, 722, 784
HapII CCGG 2 cut(s) 37, 1451
HgaI GACGC 1 cut(s) 965
HhaI GCGC 1 cut(s) 1590
Hin6I GCGC 1 cut(s) 1588
HinP1I GCGC 1 cut(s) 1588
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HinfI GANTC 8 cut(s) 40, 65, 185, 479, 789, 1201, 1641, 1718
HpaII CCGG 2 cut(s) 37, 1451
HphI GGTGA 1 cut(s) 21
Hpy166II GTNNAC 4 cut(s) 364, 773, 871, 1835
Hpy188I TCNGA 5 cut(s) 550, 741, 999, 1462, 1804
Hpy8I GTNNAC 4 cut(s) 364, 773, 871, 1835
HpyCH4III ACNGT 4 cut(s) 310, 546, 1345, 1423
HpyCH4IV ACGT 4 cut(s) 199, 318, 694, 915
HpyF10VI GCNNNNNNNGC 8 cut(s) 101, 137, 434, 527, 677, 854, 1181, 1775
HpyF3I CTNAG 3 cut(s) 998, 1011, 1432
HpySE526I ACGT 4 cut(s) 199, 318, 694, 915
HspAI GCGC 1 cut(s) 1588
KpnI GGTACC 1 cut(s) 565
Ksp22I TGATCA 3 cut(s) 927, 1144, 1465
Kzo9I GATC 5 cut(s) 121, 440, 927, 1144, 1465
LguI GCTCTTC 2 cut(s) 1227, 1329
LmnI GCTCC 2 cut(s) 854, 1463
Lsp1109I GCAGC 7 cut(s) 335, 418, 517, 774, 971, 1001, 1159
LweI GCATC 5 cut(s) 636, 658, 1078, 1240, 1676
MaeI CTAG 8 cut(s) 164, 294, 446, 665, 699, 786, 866, 1094
MaeII ACGT 4 cut(s) 199, 318, 694, 915
MaeIII GTNAC 3 cut(s) 1073, 1129, 1151
MalI GATC 5 cut(s) 123, 442, 929, 1146, 1467
MboI GATC 5 cut(s) 121, 440, 927, 1144, 1465
MfeI CAATTG 2 cut(s) 634, 1196
MflI RGATCY 1 cut(s) 440
MhlI GDGCHC 2 cut(s) 859, 1004
MlyI GAGTC 3 cut(s) 488, 783, 1635
MmeI TCCRAC 2 cut(s) 1297, 1827
Mph1103I ATGCAT 3 cut(s) 425, 1233, 1397
MseI TTAA 3 cut(s) 47, 795, 1349
MslI CAYNNNNRTG 2 cut(s) 778, 1289
MspA1I CMGCKG 2 cut(s) 245, 431
MspI CCGG 2 cut(s) 37, 1451
MspR9I CCNGG 4 cut(s) 37, 734, 810, 1452
MunI CAATTG 2 cut(s) 634, 1196
MvaI CCWGG 2 cut(s) 734, 810
MvnI CGCG 3 cut(s) 63, 573, 1588
MwoI GCNNNNNNNGC 8 cut(s) 101, 137, 434, 527, 677, 854, 1181, 1775
NciI CCSGG 2 cut(s) 37, 1452
NcoI CCATGG 1 cut(s) 1847
NdeI CATATG 2 cut(s) 1135, 1167
NdeII GATC 5 cut(s) 121, 440, 927, 1144, 1465
NlaIV GGNNCC 4 cut(s) 516, 563, 856, 1459
NmuCI GTSAC 2 cut(s) 1073, 1129
NsiI ATGCAT 3 cut(s) 425, 1233, 1397
NspI RCATGY 4 cut(s) 1399, 1650, 1810, 1861
OliI CACNNNNGTG 1 cut(s) 778
PaeI GCATGC 1 cut(s) 1399
PciI ACATGT 1 cut(s) 1806
PciSI GCTCTTC 2 cut(s) 1227, 1329
PcsI WCGNNNNNNNCGW 1 cut(s) 1212
PfeI GAWTC 5 cut(s) 40, 65, 185, 1201, 1718
PfoI TCCNGGA 1 cut(s) 35
PleI GAGTC 3 cut(s) 487, 783, 1635
PmaCI CACGTG 1 cut(s) 200
PmlI CACGTG 1 cut(s) 200
PpsI GAGTC 3 cut(s) 487, 783, 1635
Ppu21I YACGTR 2 cut(s) 200, 695
PscI ACATGT 1 cut(s) 1806
PshAI GACNNNNGTC 1 cut(s) 151
Psp124BI GAGCTC 1 cut(s) 1004
Psp6I CCWGG 2 cut(s) 732, 808
PspCI CACGTG 1 cut(s) 200
PspGI CCWGG 2 cut(s) 732, 808
PspN4I GGNNCC 4 cut(s) 516, 563, 856, 1459
PstI CTGCAG 1 cut(s) 396
PstNI CAGNNNCTG 1 cut(s) 74
PsuI RGATCY 1 cut(s) 440
RsaI GTAC 4 cut(s) 563, 569, 644, 697
RsaNI GTAC 4 cut(s) 562, 568, 643, 696
RseI CAYNNNNRTG 2 cut(s) 778, 1289
SacI GAGCTC 1 cut(s) 1004
SalI GTCGAC 1 cut(s) 362
SapI GCTCTTC 2 cut(s) 1227, 1329
SaqAI TTAA 3 cut(s) 47, 795, 1349
Sau3AI GATC 5 cut(s) 121, 440, 927, 1144, 1465
SchI GAGTC 3 cut(s) 488, 783, 1635
ScrFI CCNGG 4 cut(s) 37, 734, 810, 1452
SduI GDGCHC 2 cut(s) 859, 1004
SfaNI GCATC 5 cut(s) 636, 658, 1078, 1240, 1676
SfcI CTRYAG 2 cut(s) 392, 1662
SmiMI CAYNNNNRTG 2 cut(s) 778, 1289
SmlI CTYRAG 3 cut(s) 892, 1317, 1828
SmoI CTYRAG 3 cut(s) 892, 1317, 1828
SpeI ACTAGT 2 cut(s) 698, 865
SphI GCATGC 1 cut(s) 1399
SsiI CCGC 9 cut(s) 61, 93, 243, 429, 434, 571, 874, 1609, 1692
SspMI CTAG 8 cut(s) 164, 294, 446, 665, 699, 786, 866, 1094
SstI GAGCTC 1 cut(s) 1004
StyD4I CCNGG 4 cut(s) 35, 732, 808, 1450
StyI CCWWGG 1 cut(s) 1847
TaaI ACNGT 4 cut(s) 310, 546, 1345, 1423
TaiI ACGT 4 cut(s) 202, 321, 697, 918
TaqI TCGA 6 cut(s) 108, 363, 1042, 1234, 1287, 1670
TauI GCSGC 3 cut(s) 245, 431, 437
TfiI GAWTC 5 cut(s) 40, 65, 185, 1201, 1718
Tru1I TTAA 3 cut(s) 47, 795, 1349
Tru9I TTAA 3 cut(s) 47, 795, 1349
TscAI CASTG 3 cut(s) 396, 418, 1387
TseFI GTSAC 2 cut(s) 1073, 1129
TseI GCWGC 7 cut(s) 323, 431, 530, 762, 959, 989, 1172
Tsp45I GTSAC 2 cut(s) 1073, 1129
TspRI CASTG 3 cut(s) 396, 418, 1387
XapI RAATTY 2 cut(s) 943, 1735
XbaI TCTAGA 2 cut(s) 293, 445
XceI RCATGY 4 cut(s) 1399, 1650, 1810, 1861
XmiI GTMKAC 2 cut(s) 363, 1834
XspI CTAG 8 cut(s) 164, 294, 446, 665, 699, 786, 866, 1094
Zsp2I ATGCAT 3 cut(s) 425, 1233, 1397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.