Rw5G024800

Ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000157
Physical Location & Seq
Reverse (-)
35188406 .. 35193104
4699 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G024800.1

Sequence Viewer

Length: 1977 bp
ATGCTACATGAGAGCATAGTACGTAAGGTTGATAAGAACAATTCAGAGGACTCAAAAGAAAATACAAAGAGCTACCAGTTTGGCTCTTTTGCCCCAGTTACTATTGTCAGAGCTGGGAATTTCAACATGAAGAAGGTTGACTGGGACAATTTGTCCTCTACGTACTTACTATCCCTAGTATCAGAATCAAGGTGGAGGTTCAATTTGGAACCAATGTCTACCACACAAGCACACAATTCTATACCGCCTGGAATTGCATTGAGAGCTATGAGAGATCCAATGACATTTGCACCTTACACGCCTGGCAATAGACAGTATTACCTTGATGTATGTGTGCCGCTCAATAAGTATGCACTAAAAGGCAATTGGGAAGCTGTTGAACTAATCTTGAGGAAGGACCCGAGGCTTTTGACTGCTAGCATAACAAGCGGAGGTGACACAGTCCTTCACATAGCAGCAGGAGCAAGACAAGTTCAGTTTGCGGAGGAGCTGGTTGAAATGATGGAATTATGGATTGGAATGGAGAAATGTTCACAAGCCTTGGCAAAGCAAGGCACGAACGGAAACACTGCCTTCAGCATTGCCGCTGCAGCTGGAAGTATTGAAATTGCACAGATTATGATGAAGAAGAATGAGCATTTGCCAAAAATTCGAGGTGGTGAAGGAATGACACCACTCTATATGGCTGCCTTGTCGGGACAATCTCTAATGGCAGATTACTTGTACTCTCTAACCAAGGACATGTTGGAGGAAGCCGACCGGCAACTGTTGTTTTTAACTTGTATCGATAATGGATTGTATGATCTAGCTAGGAAGATGCTAGAAAGTGACAGAGCATTAGCTAAGGTTCGTAATATAAAAGAGGAAACAGCTTTGCATATCTTGGCTAGAATGCCTTTAGAATTCACCAGCCAAAGTCCAGGAATGTGGAGTAGACTCATCACCTCATTTATCCCAGGTTGGAAGTTCTCATACAAGAGAAACTTGAAACAAGTCAATGAAGGCCTTCAACTAGTCCAATGCCTCTGGACAGAAATCTTGAGAAATGATCATGTCGATATGATGAGATTAATTGAACATCCTACAAAATTACTGTTCGACGCTACAAAATTAGGAAATTATGAGTTCCTAGCAGTGCTTATTAACTCTTATCCTGATTTAATATGGCAACTTGATGACAAAAATCGGAGTATATTCCATGTTGCTGTTTTGCATCGTCATGCAAGTATCTTTAATTTAGTGCATGAGATAGGCTCCATCAAGGATTTCATAGTGACATTTACTGATGATGATGAGAGTAATAACATTTTACATATGGCTGCAAAATTAGCACCTCCAAATCAACTCAACCTTGTATCAGGCGCAGCTCTTCAAATGCAGCGAGAGTTGGTATGGTTTGAGGAAGTAAAAAAGATTGTACAACCTTGCTCTATAGAGATGAAAAACAAGAAAGGACAAACACCACGAGAATTGTTCACAAGTGAGCATAAAGGACTGTTGCACCAAGGAGAAGCATGGATGAAGCAAACTGCAAAGTCATGTATGATTGTTGCGGCTCTTATCGCAACTGTTGTGTTTTCAGCTGCATTTAGCGTACCTGGTGGTACATCTGATAATACAGGGCAGCCAAACTTTCTAAAAGAGACTGCCTTCCAATTCTTTGCCATAGCAGATGGAGTAGCACTCATATCCTCTTTCACTTCAATACTGATGTTCTTGCTCATCCTCACATCGCGTTATGCTGAAGGAGATTTCCTCAAATCATTACCGTTGAAGTTGATGATAGGACTCGCATCACTTTTCATCTCTATAGCATCCATGATGGTAGCTTTTAGCACAACCTTCTATTTAGACTGTCACTATGGCTTAGAATGGGTCCCCAATCTTATATTTGTATTTGTATTTGCATTTGTACCGGTTGCTTTGTTTGCTTTTCAGCCTTTTCTGCAGTTTCCTCTCAGGACCTCTCTCGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

658

Amino Acids

74.06

Weight (kDa)

6.75

Isoelectric Point (pI)

37.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 183 - 245 1.1e-06 Ankyrin repeats (3 copies)
PGG PF13962 505 - 617 1.3e-26 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54070 AT5G35830
fragaria_vesca FvH4_3g25070 FvH4_3g25080 FvH4_3g25081 FvH4_3g25081
malus_domestica MD03G1027500.v1.1 MD03G1146300.v1.1 MD03G1146800.v1.1 MD03G1146900.v1.1 MD03G1147100.v1.1 MD11G1165600.v1.1
prunus_persica Prupe.2G015800_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G016300_v2.0.a1 Prupe.2G016500_v2.0.a1 Prupe.6G131100_v2.0.a1 Prupe.6G131400_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1
pyrus_communis pycom03g10090 pycom03g10110 pycom11g13740 pycom11g13770
rosa_chinensis RchiOBHm_Chr1g0316371 RchiOBHm_Chr1g0316381 RchiOBHm_Chr1g0316401 RchiOBHm_Chr1g0316411 RchiOBHm_Chr1g0316571 RchiOBHm_Chr1g0316601 RchiOBHm_Chr5g0039341 RchiOBHm_Chr5g0041371 RchiOBHm_Chr5g0041401 RchiOBHm_Chr5g0041431 RchiOBHm_Chr5g0041471 RchiOBHm_Chr5g0045281 RchiOBHm_Chr5g0045291 RchiOBHm_Chr5g0045311 RchiOBHm_Chr5g0045371 RchiOBHm_Chr5g0045421 RchiOBHm_Chr5g0045431 RchiOBHm_Chr5g0045501 RchiOBHm_Chr5g0045551 RchiOBHm_Chr5g0045771 RchiOBHm_Chr5g0045811
rosa_laevigata RLG00000034057 RLG00000034058
rosa_multiflora Rmu_co8352751.1_g000001 Rmu_co8397575.1_g000001 Rmu_sc0000415.1_g000006 Rmu_sc0000493.1_g000057 Rmu_sc0000504.1_g000003 Rmu_sc0000594.1_g000019 Rmu_sc0000594.1_g000032 Rmu_sc0000594.1_g000066 Rmu_sc0001047.1_g000003 Rmu_sc0001047.1_g000011 Rmu_sc0001556.1_g000023 Rmu_sc0002584.1_g000003 Rmu_sc0002584.1_g000039 Rmu_sc0002584.1_g000070 Rmu_sc0002667.1_g000015 Rmu_sc0003677.1_g000003 Rmu_sc0003743.1_g000017 Rmu_sc0004520.1_g000004 Rmu_sc0004520.1_g000013 Rmu_sc0004520.1_g000027 Rmu_sc0005539.1_g000014 Rmu_sc0005539.1_g000032 Rmu_sc0006018.1_g000006 Rmu_sc0006737.1_g000020 Rmu_sc0007485.1_g000002 Rmu_sc0008729.1_g000002 Rmu_sc0008729.1_g000003 Rmu_sc0038822.1_g000001 Rmu_ssc0000398.1_g000025
rosa_roxburghii Rroxscaffold_1G00035090 Rroxscaffold_1G00035630 Rroxscaffold_1G00035640 Rroxscaffold_1G00035660 Rroxscaffold_1G00035680 Rroxscaffold_1G00035730 Rroxscaffold_1G00035750 Rroxscaffold_1G00035770 Rroxscaffold_1G00039150 Rroxscaffold_1G00039190 Rroxscaffold_1G00039220 Rroxscaffold_1G00039260 Rroxscaffold_4G00328910 Rroxscaffold_4G00328950 Rroxscaffold_4G00328960 Rroxscaffold_4G00330890
rosa_rugosa Rorug01G0009800 Rorug01G0010000.1 Rorug01G0010200 Rorug01G0012100 Rorug01G0023500 Rorug01G0023600 Rorug01G0023700 Rorug01G0024700 Rorug05G0192000 Rorug05G0192200 Rorug05G0222400 Rorug05G0222500 Rorug05G0222600.1 Rorug05G0222700.1 Rorug05G0222800.1 Rorug05G0222900.1 Rorug05G0223000.1 Rorug05G0223100.1 Rorug05G0223200 Rorug05G0223600 Rorug05G0226800 Rorug05G0226900
rosa_samantha Rh1CG019600 Rh1DG015000 Rh1DG015300 Rh1DG016900 Rh5CG315200 Rh5CG338700 Rh5DG277100 Rh5DG291500 Rh5DG292100 Rh5DG292200 Rh5DG292400 Rh5DG292600 Rh5DG322100 Rh5DG322200 Rh5DG323100 Rh5DG323300 Rh5DG326700 Rh5DG326900 Rh5DG327000
rosa_wichuraiana Rw0G021580 Rw1G001320 Rw1G001420 Rw1G002820 Rw1G002840 Rw1G002870 Rw5G024800 Rw5G026160 Rw5G026170 Rw5G026180 Rw5G026210 Rw5G026230 Rw5G028170 Rw5G028190 Rw5G028210 Rw5G028230 Rw5G028240 Rw5G028260 Rw5G028280 Rw5G028370 Rw5G028380 Rw5G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 340
AccI GTMKAC 2 cut(s) 218, 934
AccII CGCG 1 cut(s) 1735
AciI CCGC 6 cut(s) 245, 338, 429, 482, 585, 1553
AclWI GGATC 1 cut(s) 269
AcsI RAATTY 3 cut(s) 118, 648, 902
AcuI CTGAAG 2 cut(s) 559, 1764
AfaI GTAC 7 cut(s) 21, 164, 725, 1419, 1596, 1606, 1914
AflIII ACRYGT 1 cut(s) 741
AgeI ACCGGT 1 cut(s) 1915
AhdI GACNNNNNGTC 1 cut(s) 151
AhlI ACTAGT 1 cut(s) 1012
AjnI CCWGG 5 cut(s) 247, 301, 919, 955, 1597
AjuI GAANNNNNNNTTGG 2 cut(s) 498, 530
Alw26I GTCTC 1 cut(s) 1637
AlwI GGATC 1 cut(s) 269
Ama87I CYCGRG 1 cut(s) 400
AoxI GGCC 1 cut(s) 1003
ApeKI GCWGC 9 cut(s) 455, 587, 590, 686, 1319, 1364, 1378, 1583, 1624
ApoI RAATTY 3 cut(s) 118, 648, 902
AseI ATTAAT 1 cut(s) 1070
AsiGI ACCGGT 1 cut(s) 1915
Asp700I GAANNNNTTC 1 cut(s) 1005
AspLEI GCGC 1 cut(s) 1364
AspS9I GGNCC 3 cut(s) 397, 1876, 1962
AsuHPI GGTGA 4 cut(s) 446, 671, 898, 934
AsuNHI GCTAGC 1 cut(s) 416
AvaI CYCGRG 1 cut(s) 400
AvaII GGWCC 3 cut(s) 397, 1876, 1962
BarI GAAGNNNNNNTAC 2 cut(s) 956, 988
BauI CACGAG 1 cut(s) 1464
BbvI GCAGC 9 cut(s) 467, 574, 602, 673, 1306, 1376, 1390, 1570, 1636
BccI CCATC 4 cut(s) 496, 1265, 1667, 1816
BciT130I CCWGG 5 cut(s) 249, 303, 921, 957, 1599
BclI TGATCA 1 cut(s) 1048
BcoDI GTCTC 1 cut(s) 1637
BcuI ACTAGT 1 cut(s) 1012
BfaI CTAG 8 cut(s) 176, 417, 806, 810, 821, 888, 1013, 1130
BfmI CTRYAG 4 cut(s) 588, 1431, 1809, 1946
Bme1390I CCNGG 5 cut(s) 249, 303, 921, 957, 1599
Bme18I GGWCC 3 cut(s) 397, 1876, 1962
BmeRI GACNNNNNGTC 1 cut(s) 151
BmeT110I CYCGRG 1 cut(s) 400
BmgT120I GGNCC 3 cut(s) 397, 1876, 1962
BmiI GGNNCC 5 cut(s) 210, 399, 1255, 1877, 1878
BmrFI CCNGG 5 cut(s) 249, 303, 921, 957, 1599
BmrI ACTGGG 2 cut(s) 89, 151
BmsI GCATC 4 cut(s) 807, 1222, 1802, 1823
BmtI GCTAGC 1 cut(s) 420
BmuI ACTGGG 2 cut(s) 89, 151
BplI GAGNNNNNCTC 6 cut(s) 1238, 1270, 1668, 1700, 1740, 1772
Bpu10I CCTNAGC 1 cut(s) 843
BpuEI CTTGAG 2 cut(s) 409, 1060
Bsa29I ATCGAT 1 cut(s) 786
BsaAI YACGTR 2 cut(s) 23, 162
BsaJI CCNNGG 5 cut(s) 401, 540, 735, 955, 1504
BsaWI WCCGGW 1 cut(s) 1915
Bse118I RCCGGY 2 cut(s) 759, 1915
Bse1I ACTGG 3 cut(s) 76, 95, 146
Bse3DI GCAATG 1 cut(s) 579
BseBI CCWGG 5 cut(s) 249, 303, 921, 957, 1599
BseCI ATCGAT 1 cut(s) 786
BseDI CCNNGG 5 cut(s) 401, 540, 735, 955, 1504
BseGI GGATG 4 cut(s) 1078, 1524, 1722, 1814
BseMI GCAATG 1 cut(s) 579
BseMII CTCAG 1 cut(s) 1972
BseNI ACTGG 3 cut(s) 76, 95, 146
BseRI GAGGAG 1 cut(s) 500
BseXI GCAGC 9 cut(s) 467, 574, 602, 673, 1306, 1376, 1390, 1570, 1636
BseYI CCCAGC 1 cut(s) 113
Bsh1236I CGCG 1 cut(s) 1735
Bsh1285I CGRYCG 1 cut(s) 760
BshFI GGCC 1 cut(s) 1005
BshTI ACCGGT 1 cut(s) 1915
BshVI ATCGAT 1 cut(s) 786
BsiEI CGRYCG 1 cut(s) 760
BsiHKCI CYCGRG 1 cut(s) 400
BsiSI CCGG 2 cut(s) 760, 1916
BslFI GGGAC 3 cut(s) 158, 711, 1862
BsmAI GTCTC 1 cut(s) 1637
BsmFI GGGAC 3 cut(s) 158, 711, 1862
BsmI GAATGC 1 cut(s) 897
BsnI GGCC 1 cut(s) 1005
BsoBI CYCGRG 1 cut(s) 400
Bsp1407I TGTACA 1 cut(s) 1417
Bsp143I GATC 3 cut(s) 274, 802, 1048
BspACI CCGC 6 cut(s) 245, 338, 429, 482, 585, 1553
BspANI GGCC 1 cut(s) 1005
BspCNI CTCAG 1 cut(s) 1971
BspDI ATCGAT 1 cut(s) 786
BspFNI CGCG 1 cut(s) 1735
BspLI GGNNCC 5 cut(s) 210, 399, 1255, 1877, 1878
BspMAI CTGCAG 2 cut(s) 592, 1950
BspOI GCTAGC 1 cut(s) 420
BspPI GGATC 1 cut(s) 269
BspQI GCTCTTC 1 cut(s) 1374
BsrBI CCGCTC 1 cut(s) 340
BsrDI GCAATG 1 cut(s) 579
BsrFI RCCGGY 2 cut(s) 759, 1915
BsrGI TGTACA 1 cut(s) 1417
BsrI ACTGG 3 cut(s) 76, 95, 146
BssAI RCCGGY 2 cut(s) 759, 1915
BssECI CCNNGG 5 cut(s) 401, 540, 735, 955, 1504
BssMI GATC 3 cut(s) 274, 802, 1048
BssSI CACGAG 1 cut(s) 1464
BssT1I CCWWGG 3 cut(s) 540, 735, 1504
Bst2BI CACGAG 1 cut(s) 1464
Bst2UI CCWGG 5 cut(s) 249, 303, 921, 957, 1599
Bst4CI ACNGT 8 cut(s) 315, 442, 768, 1095, 1497, 1570, 1770, 1856
Bst6I CTCTTC 1 cut(s) 1374
BstAUI TGTACA 1 cut(s) 1417
BstBAI YACGTR 2 cut(s) 23, 162
BstC8I GCNNGC 1 cut(s) 418
BstDEI CTNAG 3 cut(s) 843, 1867, 1958
BstF5I GGATG 4 cut(s) 1078, 1524, 1722, 1814
BstFNI CGCG 1 cut(s) 1735
BstHHI GCGC 1 cut(s) 1364
BstKTI GATC 3 cut(s) 277, 805, 1051
BstMAI GTCTC 1 cut(s) 1637
BstMBI GATC 3 cut(s) 274, 802, 1048
BstMCI CGRYCG 1 cut(s) 760
BstMWI GCNNNNNNNGC 8 cut(s) 263, 426, 461, 590, 1328, 1562, 1928, 1945
BstNI CCWGG 5 cut(s) 249, 303, 921, 957, 1599
BstNSI RCATGY 1 cut(s) 745
BstSCI CCNGG 5 cut(s) 247, 301, 919, 955, 1597
BstSFI CTRYAG 4 cut(s) 588, 1431, 1809, 1946
BstSNI TACGTA 2 cut(s) 23, 162
BstUI CGCG 1 cut(s) 1735
BstV1I GCAGC 9 cut(s) 467, 574, 602, 673, 1306, 1376, 1390, 1570, 1636
BstX2I RGATCY 1 cut(s) 274
BstXI CCANNNNNNTGG 1 cut(s) 927
BstYI RGATCY 1 cut(s) 274
Bsu15I ATCGAT 1 cut(s) 786
BsuRI GGCC 1 cut(s) 1005
BsuTUI ATCGAT 1 cut(s) 786
BtgZI GCGATG 1 cut(s) 1716
BtsCI GGATG 4 cut(s) 1078, 1524, 1722, 1814
BtsI GCAGTG 2 cut(s) 567, 1140
BtsIMutI CAGTG 2 cut(s) 567, 1140
Cac8I GCNNGC 1 cut(s) 418
CfoI GCGC 1 cut(s) 1364
Cfr10I RCCGGY 2 cut(s) 759, 1915
Cfr13I GGNCC 3 cut(s) 397, 1876, 1962
ClaI ATCGAT 1 cut(s) 786
CseI GACGC 1 cut(s) 1109
CsiI ACCWGGT 1 cut(s) 1597
Csp6I GTAC 7 cut(s) 20, 163, 724, 1418, 1595, 1605, 1913
CspAI ACCGGT 1 cut(s) 1915
CspCI CAANNNNNGTGG 2 cut(s) 1452, 1487
CviQI GTAC 7 cut(s) 20, 163, 724, 1418, 1595, 1605, 1913
DdeI CTNAG 3 cut(s) 843, 1867, 1958
DpnI GATC 3 cut(s) 276, 804, 1050
DpnII GATC 3 cut(s) 274, 802, 1048
DriI GACNNNNNGTC 1 cut(s) 151
Eam1104I CTCTTC 1 cut(s) 1374
Eam1105I GACNNNNNGTC 1 cut(s) 151
EarI CTCTTC 1 cut(s) 1374
Eco105I TACGTA 2 cut(s) 23, 162
Eco130I CCWWGG 3 cut(s) 540, 735, 1504
Eco147I AGGCCT 1 cut(s) 1005
Eco47I GGWCC 3 cut(s) 397, 1876, 1962
Eco57I CTGAAG 2 cut(s) 559, 1764
Eco88I CYCGRG 1 cut(s) 400
EcoO109I RGGNCCY 3 cut(s) 397, 1876, 1962
EcoRI GAATTC 1 cut(s) 902
EcoRII CCWGG 5 cut(s) 247, 301, 919, 955, 1597
EcoT14I CCWWGG 3 cut(s) 540, 735, 1504
ErhI CCWWGG 3 cut(s) 540, 735, 1504
FalI AAGNNNNNCTT 2 cut(s) 968, 1000
FaqI GGGAC 3 cut(s) 158, 711, 1862
FauNDI CATATG 1 cut(s) 1314
FbaI TGATCA 1 cut(s) 1048
FblI GTMKAC 2 cut(s) 218, 934
FokI GGATG 4 cut(s) 1065, 1531, 1709, 1801
FspBI CTAG 8 cut(s) 176, 417, 806, 810, 821, 888, 1013, 1130
GlaI GCGC 1 cut(s) 1363
GsaI CCCAGC 1 cut(s) 117
HaeIII GGCC 1 cut(s) 1005
HapII CCGG 2 cut(s) 760, 1916
HgaI GACGC 1 cut(s) 1109
HhaI GCGC 1 cut(s) 1364
Hin6I GCGC 1 cut(s) 1362
HinP1I GCGC 1 cut(s) 1362
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HinfI GANTC 4 cut(s) 50, 185, 936, 1788
HpaII CCGG 2 cut(s) 760, 1916
HphI GGTGA 4 cut(s) 446, 671, 898, 934
Hpy166II GTNNAC 5 cut(s) 139, 219, 533, 935, 1476
Hpy188I TCNGA 6 cut(s) 46, 110, 184, 1188, 1612, 1976
Hpy188III TCNNGA 6 cut(s) 388, 696, 1027, 1039, 1154, 1960
Hpy8I GTNNAC 5 cut(s) 139, 219, 533, 935, 1476
Hpy99I CGWCG 1 cut(s) 1103
HpyCH4III ACNGT 8 cut(s) 315, 442, 768, 1095, 1497, 1570, 1770, 1856
HpyCH4IV ACGT 2 cut(s) 22, 161
HpyF10VI GCNNNNNNNGC 8 cut(s) 263, 426, 461, 590, 1328, 1562, 1928, 1945
HpyF3I CTNAG 3 cut(s) 843, 1867, 1958
HpySE526I ACGT 2 cut(s) 22, 161
HspAI GCGC 1 cut(s) 1362
KflI GGGWCCC 1 cut(s) 1876
Ksp22I TGATCA 1 cut(s) 1048
Kzo9I GATC 3 cut(s) 274, 802, 1048
LguI GCTCTTC 1 cut(s) 1374
LmnI GCTCC 3 cut(s) 461, 487, 1259
Lsp1109I GCAGC 9 cut(s) 467, 574, 602, 673, 1306, 1376, 1390, 1570, 1636
LweI GCATC 4 cut(s) 807, 1222, 1802, 1823
MabI ACCWGGT 1 cut(s) 1597
MaeI CTAG 8 cut(s) 176, 417, 806, 810, 821, 888, 1013, 1130
MaeII ACGT 2 cut(s) 22, 161
MaeIII GTNAC 5 cut(s) 97, 434, 827, 1273, 1856
MalI GATC 3 cut(s) 276, 804, 1050
MbiI CCGCTC 1 cut(s) 340
MboI GATC 3 cut(s) 274, 802, 1048
MboII GAAGA 5 cut(s) 142, 637, 640, 826, 1361
MfeI CAATTG 1 cut(s) 364
MflI RGATCY 1 cut(s) 274
MlyI GAGTC 3 cut(s) 44, 930, 1782
MmeI TCCRAC 2 cut(s) 726, 941
MroXI GAANNNNTTC 1 cut(s) 1005
MseI TTAA 5 cut(s) 776, 1070, 1143, 1160, 1233
MslI CAYNNNNRTG 1 cut(s) 1218
MspA1I CMGCKG 3 cut(s) 587, 593, 1583
MspI CCGG 2 cut(s) 760, 1916
MspR9I CCNGG 5 cut(s) 249, 303, 921, 957, 1599
MunI CAATTG 1 cut(s) 364
Mva1269I GAATGC 1 cut(s) 897
MvaI CCWGG 5 cut(s) 249, 303, 921, 957, 1599
MvnI CGCG 1 cut(s) 1735
MwoI GCNNNNNNNGC 8 cut(s) 263, 426, 461, 590, 1328, 1562, 1928, 1945
NdeI CATATG 1 cut(s) 1314
NdeII GATC 3 cut(s) 274, 802, 1048
NheI GCTAGC 1 cut(s) 416
NlaIV GGNNCC 5 cut(s) 210, 399, 1255, 1877, 1878
NmuCI GTSAC 4 cut(s) 434, 827, 1273, 1856
NspI RCATGY 1 cut(s) 745
PceI AGGCCT 1 cut(s) 1005
PciI ACATGT 1 cut(s) 741
PciSI GCTCTTC 1 cut(s) 1374
PctI GAATGC 1 cut(s) 897
PdmI GAANNNNTTC 1 cut(s) 1005
PfeI GAWTC 1 cut(s) 185
PflFI GACNNNGTC 1 cut(s) 440
PfoI TCCNGGA 1 cut(s) 919
PinAI ACCGGT 1 cut(s) 1915
PleI GAGTC 3 cut(s) 44, 930, 1782
PpsI GAGTC 3 cut(s) 44, 930, 1782
Ppu21I YACGTR 2 cut(s) 23, 162
PpuMI RGGWCCY 3 cut(s) 397, 1876, 1962
PscI ACATGT 1 cut(s) 741
PshBI ATTAAT 1 cut(s) 1070
Psp5II RGGWCCY 3 cut(s) 397, 1876, 1962
Psp6I CCWGG 5 cut(s) 247, 301, 919, 955, 1597
PspFI CCCAGC 1 cut(s) 113
PspGI CCWGG 5 cut(s) 247, 301, 919, 955, 1597
PspN4I GGNNCC 5 cut(s) 210, 399, 1255, 1877, 1878
PspPI GGNCC 3 cut(s) 397, 1876, 1962
PspPPI RGGWCCY 3 cut(s) 397, 1876, 1962
PstI CTGCAG 2 cut(s) 592, 1950
PsuI RGATCY 1 cut(s) 274
PsyI GACNNNGTC 1 cut(s) 440
PvuII CAGCTG 2 cut(s) 593, 1583
RsaI GTAC 7 cut(s) 21, 164, 725, 1419, 1596, 1606, 1914
RsaNI GTAC 7 cut(s) 20, 163, 724, 1418, 1595, 1605, 1913
RseI CAYNNNNRTG 1 cut(s) 1218
SapI GCTCTTC 1 cut(s) 1374
SaqAI TTAA 5 cut(s) 776, 1070, 1143, 1160, 1233
Sau3AI GATC 3 cut(s) 274, 802, 1048
Sau96I GGNCC 3 cut(s) 397, 1876, 1962
SchI GAGTC 3 cut(s) 44, 930, 1782
ScrFI CCNGG 5 cut(s) 249, 303, 921, 957, 1599
SexAI ACCWGGT 1 cut(s) 1597
SfaNI GCATC 4 cut(s) 807, 1222, 1802, 1823
SfcI CTRYAG 4 cut(s) 588, 1431, 1809, 1946
SinI GGWCC 3 cut(s) 397, 1876, 1962
SmiMI CAYNNNNRTG 1 cut(s) 1218
SmlI CTYRAG 2 cut(s) 388, 1039
SmoI CTYRAG 2 cut(s) 388, 1039
SnaBI TACGTA 2 cut(s) 23, 162
SpeI ACTAGT 1 cut(s) 1012
SseBI AGGCCT 1 cut(s) 1005
SsiI CCGC 6 cut(s) 245, 338, 429, 482, 585, 1553
SspMI CTAG 8 cut(s) 176, 417, 806, 810, 821, 888, 1013, 1130
StuI AGGCCT 1 cut(s) 1005
StyD4I CCNGG 5 cut(s) 247, 301, 919, 955, 1597
StyI CCWWGG 3 cut(s) 540, 735, 1504
TaaI ACNGT 8 cut(s) 315, 442, 768, 1095, 1497, 1570, 1770, 1856
TaiI ACGT 2 cut(s) 25, 164
TaqI TCGA 4 cut(s) 652, 786, 1056, 1098
TatI WGTACW 2 cut(s) 723, 1417
TauI GCSGC 3 cut(s) 340, 587, 1556
TfiI GAWTC 1 cut(s) 185
Tru1I TTAA 5 cut(s) 776, 1070, 1143, 1160, 1233
Tru9I TTAA 5 cut(s) 776, 1070, 1143, 1160, 1233
TscAI CASTG 2 cut(s) 574, 1140
TseFI GTSAC 4 cut(s) 434, 827, 1273, 1856
TseI GCWGC 9 cut(s) 455, 587, 590, 686, 1319, 1364, 1378, 1583, 1624
Tsp45I GTSAC 4 cut(s) 434, 827, 1273, 1856
TspDTI ATGAA 7 cut(s) 143, 638, 1014, 1258, 1454, 1535, 1792
TspGWI ACGGA 1 cut(s) 576
TspRI CASTG 2 cut(s) 574, 1140
Tth111I GACNNNGTC 1 cut(s) 440
VpaK11BI GGWCC 3 cut(s) 397, 1876, 1962
VspI ATTAAT 1 cut(s) 1070
XapI RAATTY 3 cut(s) 118, 648, 902
XceI RCATGY 1 cut(s) 745
XcmI CCANNNNNNNNNTGG 1 cut(s) 742
XmiI GTMKAC 2 cut(s) 218, 934
XmnI GAANNNNTTC 1 cut(s) 1005
XspI CTAG 8 cut(s) 176, 417, 806, 810, 821, 888, 1013, 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.