Rroxscaffold_1G00035640

Ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000157
Physical Location & Seq
Forward (+)
51777769 .. 51781890
4122 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00035640.1

Sequence Viewer

Length: 1797 bp
ATGTGTACACAATTTGTTCAATTTGAAACCATGTCTATCCCACAAGCACAACATGTACCACCCCCTGGGATTCAATTAGCCACCGCAAAGGGTGATCCAGGAACTGGTGGGCTAGAAAACCAATCTTCAAATTTCACTACCATCGACATTTTGGCAGATCTACCTAAATCTAAGACACCTTCACTTCATCTACTGGAACACAAAAATAATCGAACACTTCTGAGTGCTAGCATATCAAGAGGGTGGGACACTGTTCTTCATATTGCAGCAGGAGCAAGACATGTTCACTTTGTGGAGAAACTGGTTGAAATCATGGACAAAGAACACTTGGCACTCCAAGACATAAATGGAAACACTGCATTATGTATTGCTGCCGCGGCAGGATCAGTAGACATTGTCCAAATTTTGATAAAACATAATGATTCCTTGCTGACAATTCGAGGAGGTCAGAAAATGGCACCACCCTATATGGCTGCAATGTTAGGACAGTCAGAAATGGCATGGTACCTGTACCCTAAAAGTAAAAAGATGATGGAGAAGACAGACCTTGAGAACTTGTTTTTTTCGTGCATCAACAATGGTTTATATGATTTAGCTTTACAGTTGCTAGATACTGATAGAGCATTAGCCAAGGCACGTACTGATCGGCCGAAAAATAAAACCGCCCTGCATATCTTGGCTCGAAGGCCTTCAGTATTTGGAAGTCAAAGTCTAGGAATGTGGGGTAGACTCATGAAATCATGTTTTAAGTTTGCATTCAAAAGAAATTTAAATCAAGCTGATAAAGCACTTCACCTAGTCCAACGCCTTTGGGAAGAAATTTTGAAATATGAGCATGATGACGTGATGAGGTTGATCAAATATCCCTCTAATTTAATATTTGATGCAACAGAATTAGGGAATTATGAGTTCCTGGCAGCGCTTATGAGCTCTTATCCTGAGTTAGTATGGGAAACTGATGAAAAAAATCGAACTATAATCCATGTTGCAGTTTTGCATCGTCATGCAAGTATCTTCAATCTAGTGCATGAGATAGGCTCAATCAAGGATGTCATAGTGACTTACGAGGATGATCAGGGTAATAATATAGTGCATATGGTTGCAAAAGCAGCACCTCAAAACCAACTGAATTTGGTGTCAGGCGTAGCTCTTCAAATGCAGCGAGAGTTAGTATGGTTTGAGGAAATAAAGAAGATTGTGCAACCTCAATATATAGAGATGGAAAACAAGAAAGGAAAAACACCTCAAGAATTATTCACTGAAGAGCATAAGAAGTTAATGCGTCAAGGAGAAAAATGGATGAAGGACACTGCAACTTCATGCTTGCTTGTTGCGACTATTATTGCAACTGTTGTGTTTTCAGCTGCATTTAGTATACCAGGTGGCGCAGATGATCATACTGGAAAACCAAACTTTTTGAAAGAGAAAGCTTTTTTATACTTTATCGTAGCCGACGGAGTGGCACTCTTTTCTTCTTCAACTGCAATGCTGATGTTCTTGTTCATCCTTACCTCTCGGTATGCAGAAAATGATTTCCTCAAGTCCTTGCCCTTGAAGTTGATGGTAGGACTCACTTGCCTCTTCATCTCAATAGCATCTATGATGATGGCTTTCAGCACCGCCTTTTATTTGTCTTGCCAATATGGATCAAGATGGGTTCCAAATCTTACATTTTTCTTTGCAATTGTCCCTGTTGCTTTATATGCATTTCTACAATTCCCTCTTGTGTCTGATATGTCCTCCTCCACATTTTGTTCAAGTCTTTTATTTCAGCCATGGAAACGTATGATATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 PGG

Protein Analysis

598

Amino Acids

67.38

Weight (kDa)

8.0

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 80 - 140 7.2e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 86 - 174 1.2e-09 Ankyrin repeats (3 copies)
PGG PF13962 431 - 544 8.8e-27 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54070 AT5G35830
fragaria_vesca FvH4_3g25070 FvH4_3g25080 FvH4_3g25081 FvH4_3g25081
malus_domestica MD03G1027500.v1.1 MD03G1146300.v1.1 MD03G1146800.v1.1 MD03G1146900.v1.1 MD03G1147100.v1.1 MD11G1165600.v1.1
prunus_persica Prupe.2G015800_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G015900_v2.0.a1 Prupe.2G016300_v2.0.a1 Prupe.2G016500_v2.0.a1 Prupe.6G131100_v2.0.a1 Prupe.6G131400_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131500_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131600_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1 Prupe.6G131800_v2.0.a1
pyrus_communis pycom03g10090 pycom03g10110 pycom11g13740 pycom11g13770
rosa_chinensis RchiOBHm_Chr1g0316371 RchiOBHm_Chr1g0316381 RchiOBHm_Chr1g0316401 RchiOBHm_Chr1g0316411 RchiOBHm_Chr1g0316571 RchiOBHm_Chr1g0316601 RchiOBHm_Chr5g0039341 RchiOBHm_Chr5g0041371 RchiOBHm_Chr5g0041401 RchiOBHm_Chr5g0041431 RchiOBHm_Chr5g0041471 RchiOBHm_Chr5g0045281 RchiOBHm_Chr5g0045291 RchiOBHm_Chr5g0045311 RchiOBHm_Chr5g0045371 RchiOBHm_Chr5g0045421 RchiOBHm_Chr5g0045431 RchiOBHm_Chr5g0045501 RchiOBHm_Chr5g0045551 RchiOBHm_Chr5g0045771 RchiOBHm_Chr5g0045811
rosa_laevigata RLG00000034057 RLG00000034058
rosa_multiflora Rmu_co8352751.1_g000001 Rmu_co8397575.1_g000001 Rmu_sc0000415.1_g000006 Rmu_sc0000493.1_g000057 Rmu_sc0000504.1_g000003 Rmu_sc0000594.1_g000019 Rmu_sc0000594.1_g000032 Rmu_sc0000594.1_g000066 Rmu_sc0001047.1_g000003 Rmu_sc0001047.1_g000011 Rmu_sc0001556.1_g000023 Rmu_sc0002584.1_g000003 Rmu_sc0002584.1_g000039 Rmu_sc0002584.1_g000070 Rmu_sc0002667.1_g000015 Rmu_sc0003677.1_g000003 Rmu_sc0003743.1_g000017 Rmu_sc0004520.1_g000004 Rmu_sc0004520.1_g000013 Rmu_sc0004520.1_g000027 Rmu_sc0005539.1_g000014 Rmu_sc0005539.1_g000032 Rmu_sc0006018.1_g000006 Rmu_sc0006737.1_g000020 Rmu_sc0007485.1_g000002 Rmu_sc0008729.1_g000002 Rmu_sc0008729.1_g000003 Rmu_sc0038822.1_g000001 Rmu_ssc0000398.1_g000025
rosa_roxburghii Rroxscaffold_1G00035090 Rroxscaffold_1G00035630 Rroxscaffold_1G00035640 Rroxscaffold_1G00035660 Rroxscaffold_1G00035680 Rroxscaffold_1G00035730 Rroxscaffold_1G00035750 Rroxscaffold_1G00035770 Rroxscaffold_1G00039150 Rroxscaffold_1G00039190 Rroxscaffold_1G00039220 Rroxscaffold_1G00039260 Rroxscaffold_4G00328910 Rroxscaffold_4G00328950 Rroxscaffold_4G00328960 Rroxscaffold_4G00330890
rosa_rugosa Rorug01G0009800 Rorug01G0010000.1 Rorug01G0010200 Rorug01G0012100 Rorug01G0023500 Rorug01G0023600 Rorug01G0023700 Rorug01G0024700 Rorug05G0192000 Rorug05G0192200 Rorug05G0222400 Rorug05G0222500 Rorug05G0222600.1 Rorug05G0222700.1 Rorug05G0222800.1 Rorug05G0222900.1 Rorug05G0223000.1 Rorug05G0223100.1 Rorug05G0223200 Rorug05G0223600 Rorug05G0226800 Rorug05G0226900
rosa_samantha Rh1CG019600 Rh1DG015000 Rh1DG015300 Rh1DG016900 Rh5CG315200 Rh5CG338700 Rh5DG277100 Rh5DG291500 Rh5DG292100 Rh5DG292200 Rh5DG292400 Rh5DG292600 Rh5DG322100 Rh5DG322200 Rh5DG323100 Rh5DG323300 Rh5DG326700 Rh5DG326900 Rh5DG327000
rosa_wichuraiana Rw0G021580 Rw1G001320 Rw1G001420 Rw1G002820 Rw1G002840 Rw1G002870 Rw5G024800 Rw5G026160 Rw5G026170 Rw5G026180 Rw5G026210 Rw5G026230 Rw5G028170 Rw5G028190 Rw5G028210 Rw5G028230 Rw5G028240 Rw5G028260 Rw5G028280 Rw5G028370 Rw5G028380 Rw5G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 504
AccB1I GGYRCC 2 cut(s) 457, 504
AccB7I CCANNNNNTGG 2 cut(s) 65, 104
AccI GTMKAC 3 cut(s) 390, 727, 1375
AccII CGCG 1 cut(s) 377
AciI CCGC 5 cut(s) 84, 375, 377, 663, 1620
AclWI GGATC 3 cut(s) 89, 391, 1654
AcoI YGGCCR 1 cut(s) 647
AcsI RAATTY 5 cut(s) 130, 402, 766, 819, 1129
AcuI CTGAAG 2 cut(s) 675, 1281
AdeI CACNNNGTG 1 cut(s) 292
AfaI GTAC 5 cut(s) 7, 57, 506, 512, 640
AfeI AGCGCT 1 cut(s) 921
AfiI CCNNNNNNNGG 2 cut(s) 65, 104
AflIII ACRYGT 2 cut(s) 52, 280
AjiI CACGTC 1 cut(s) 844
AjnI CCWGG 4 cut(s) 64, 97, 912, 1378
AluBI AGCT 6 cut(s) 596, 779, 930, 1148, 1364, 1430
AluI AGCT 6 cut(s) 596, 779, 930, 1148, 1364, 1430
Alw21I GWGCWC 1 cut(s) 932
AlwI GGATC 3 cut(s) 89, 391, 1654
AlwNI CAGNNNCTG 1 cut(s) 104
Aor51HI AGCGCT 1 cut(s) 921
AoxI GGCC 2 cut(s) 647, 686
ApeKI GCWGC 7 cut(s) 266, 371, 473, 917, 1109, 1159, 1364
ApoI RAATTY 5 cut(s) 130, 402, 766, 819, 1129
Asp700I GAANNNNTTC 1 cut(s) 688
Asp718I GGTACC 1 cut(s) 504
AspLEI GCGC 2 cut(s) 922, 1388
AsuHPI GGTGA 2 cut(s) 104, 785
AsuNHI GCTAGC 1 cut(s) 227
BanI GGYRCC 2 cut(s) 457, 504
BanII GRGCYC 1 cut(s) 932
BbsI GAAGAC 1 cut(s) 545
Bbv12I GWGCWC 1 cut(s) 932
BbvI GCAGC 7 cut(s) 278, 358, 460, 929, 1121, 1171, 1351
BccI CCATC 6 cut(s) 149, 526, 1213, 1555, 1600, 1647
BciT130I CCWGG 4 cut(s) 66, 99, 914, 1380
BclI TGATCA 3 cut(s) 855, 1072, 1393
BfaI CTAG 7 cut(s) 113, 228, 608, 713, 797, 1022, 1795
BfoI RGCGCY 1 cut(s) 923
BglII AGATCT 1 cut(s) 157
BisI GCNGC 9 cut(s) 267, 372, 375, 378, 474, 918, 1110, 1160, 1365
BlsI GCNGC 9 cut(s) 268, 373, 376, 379, 475, 919, 1111, 1161, 1366
Bme1390I CCNGG 4 cut(s) 66, 99, 914, 1380
BmgBI CACGTC 1 cut(s) 844
BmiI GGNNCC 3 cut(s) 459, 506, 1659
BmrFI CCNGG 4 cut(s) 66, 99, 914, 1380
BmsI GCATC 4 cut(s) 579, 874, 1006, 1604
BmtI GCTAGC 1 cut(s) 231
BpiI GAAGAC 1 cut(s) 545
BplI GAGNNNNNCTC 4 cut(s) 1022, 1054, 1449, 1481
BpuEI CTTGAG 3 cut(s) 569, 1230, 1523
BsaAI YACGTR 1 cut(s) 638
BsaJI CCNNGG 5 cut(s) 64, 65, 375, 630, 1775
Bsc4I CCNNNNNNNGG 2 cut(s) 65, 104
Bse1I ACTGG 4 cut(s) 109, 198, 306, 1405
Bse3DI GCAATG 2 cut(s) 483, 1491
BseBI CCWGG 4 cut(s) 66, 99, 914, 1380
BseDI CCNNGG 5 cut(s) 64, 65, 375, 630, 1775
BseGI GGATG 4 cut(s) 1054, 1075, 1305, 1503
BseLI CCNNNNNNNGG 2 cut(s) 65, 104
BseMI GCAATG 2 cut(s) 483, 1491
BseMII CTCAG 2 cut(s) 212, 930
BseNI ACTGG 4 cut(s) 109, 198, 306, 1405
BseRI GAGGAG 2 cut(s) 456, 1732
BseX3I CGGCCG 1 cut(s) 647
BseXI GCAGC 7 cut(s) 278, 358, 460, 929, 1121, 1171, 1351
Bsh1236I CGCG 1 cut(s) 377
Bsh1285I CGRYCG 1 cut(s) 650
BshFI GGCC 2 cut(s) 649, 688
BshNI GGYRCC 2 cut(s) 457, 504
BsiEI CGRYCG 1 cut(s) 650
BsiHKAI GWGCWC 1 cut(s) 932
BslFI GGGAC 2 cut(s) 260, 1673
BslI CCNNNNNNNGG 2 cut(s) 65, 104
BsmFI GGGAC 2 cut(s) 260, 1673
BsmI GAATGC 1 cut(s) 755
BsnI GGCC 2 cut(s) 649, 688
Bsp1286I GDGCHC 1 cut(s) 932
Bsp1407I TGTACA 1 cut(s) 5
Bsp143I GATC 8 cut(s) 94, 157, 383, 643, 855, 1072, 1393, 1646
Bsp19I CCATGG 1 cut(s) 1775
BspACI CCGC 5 cut(s) 84, 375, 377, 663, 1620
BspANI GGCC 2 cut(s) 649, 688
BspCNI CTCAG 2 cut(s) 213, 931
BspFNI CGCG 1 cut(s) 377
BspHI TCATGA 1 cut(s) 732
BspLI GGNNCC 3 cut(s) 459, 506, 1659
BspOI GCTAGC 1 cut(s) 231
BspPI GGATC 3 cut(s) 89, 391, 1654
BspQI GCTCTTC 2 cut(s) 1155, 1257
BspT107I GGYRCC 2 cut(s) 457, 504
BsrDI GCAATG 2 cut(s) 483, 1491
BsrGI TGTACA 1 cut(s) 5
BsrI ACTGG 4 cut(s) 109, 198, 306, 1405
BssECI CCNNGG 5 cut(s) 64, 65, 375, 630, 1775
BssMI GATC 8 cut(s) 94, 157, 383, 643, 855, 1072, 1393, 1646
BssNAI GTATAC 1 cut(s) 1376
BssT1I CCWWGG 2 cut(s) 630, 1775
Bst1107I GTATAC 1 cut(s) 1376
Bst2UI CCWGG 4 cut(s) 66, 99, 914, 1380
Bst4CI ACNGT 4 cut(s) 253, 489, 603, 1351
Bst6I CTCTTC 3 cut(s) 1155, 1257, 1586
BstAUI TGTACA 1 cut(s) 5
BstBAI YACGTR 1 cut(s) 638
BstC8I GCNNGC 2 cut(s) 229, 1325
BstDEI CTNAG 3 cut(s) 171, 221, 939
BstDSI CCRYGG 2 cut(s) 375, 1775
BstF5I GGATG 4 cut(s) 1054, 1075, 1305, 1503
BstFNI CGCG 1 cut(s) 377
BstH2I RGCGCY 1 cut(s) 923
BstHHI GCGC 2 cut(s) 922, 1388
BstKTI GATC 8 cut(s) 97, 160, 386, 646, 858, 1075, 1396, 1649
BstMBI GATC 8 cut(s) 94, 157, 383, 643, 855, 1072, 1393, 1646
BstMCI CGRYCG 1 cut(s) 650
BstMWI GCNNNNNNNGC 5 cut(s) 272, 377, 785, 1109, 1703
BstNI CCWGG 4 cut(s) 66, 99, 914, 1380
BstNSI RCATGY 2 cut(s) 56, 284
BstSCI CCNGG 4 cut(s) 64, 97, 912, 1378
BstUI CGCG 1 cut(s) 377
BstV1I GCAGC 7 cut(s) 278, 358, 460, 929, 1121, 1171, 1351
BstV2I GAAGAC 1 cut(s) 545
BstX2I RGATCY 1 cut(s) 157
BstYI RGATCY 1 cut(s) 157
BstZ17I GTATAC 1 cut(s) 1376
BstZI CGGCCG 1 cut(s) 647
BsuRI GGCC 2 cut(s) 649, 688
BtgI CCRYGG 2 cut(s) 375, 1775
BtrI CACGTC 1 cut(s) 844
BtsCI GGATG 4 cut(s) 1054, 1075, 1305, 1503
BtsI GCAGTG 2 cut(s) 354, 1308
BtsIMutI CAGTG 4 cut(s) 249, 354, 1257, 1308
Cac8I GCNNGC 2 cut(s) 229, 1325
CaiI CAGNNNCTG 1 cut(s) 104
CciI TCATGA 1 cut(s) 732
CfoI GCGC 2 cut(s) 922, 1388
Cfr42I CCGCGG 1 cut(s) 378
CseI GACGC 1 cut(s) 1271
CsiI ACCWGGT 1 cut(s) 1378
Csp6I GTAC 5 cut(s) 6, 56, 505, 511, 639
CviQI GTAC 5 cut(s) 6, 56, 505, 511, 639
DdeI CTNAG 3 cut(s) 171, 221, 939
DpnI GATC 8 cut(s) 96, 159, 385, 645, 857, 1074, 1395, 1648
DpnII GATC 8 cut(s) 94, 157, 383, 643, 855, 1072, 1393, 1646
DraI TTTAAA 1 cut(s) 771
DraIII CACNNNGTG 1 cut(s) 292
EaeI YGGCCR 1 cut(s) 647
EagI CGGCCG 1 cut(s) 647
Eam1104I CTCTTC 3 cut(s) 1155, 1257, 1586
EarI CTCTTC 3 cut(s) 1155, 1257, 1586
Ecl136II GAGCTC 1 cut(s) 930
EclXI CGGCCG 1 cut(s) 647
Eco130I CCWWGG 2 cut(s) 630, 1775
Eco147I AGGCCT 1 cut(s) 688
Eco24I GRGCYC 1 cut(s) 932
Eco47III AGCGCT 1 cut(s) 921
Eco52I CGGCCG 1 cut(s) 647
Eco53kI GAGCTC 1 cut(s) 930
Eco57I CTGAAG 2 cut(s) 675, 1281
EcoICRI GAGCTC 1 cut(s) 930
EcoRII CCWGG 4 cut(s) 64, 97, 912, 1378
EcoT14I CCWWGG 2 cut(s) 630, 1775
EcoT22I ATGCAT 1 cut(s) 1708
EcoT38I GRGCYC 1 cut(s) 932
ErhI CCWWGG 2 cut(s) 630, 1775
FaqI GGGAC 2 cut(s) 260, 1673
FauNDI CATATG 1 cut(s) 1095
FbaI TGATCA 3 cut(s) 855, 1072, 1393
FblI GTMKAC 3 cut(s) 390, 727, 1375
Fnu4HI GCNGC 9 cut(s) 267, 372, 375, 378, 474, 918, 1110, 1160, 1365
FokI GGATG 4 cut(s) 1061, 1082, 1312, 1490
FriOI GRGCYC 1 cut(s) 932
Fsp4HI GCNGC 9 cut(s) 267, 372, 375, 378, 474, 918, 1110, 1160, 1365
FspBI CTAG 7 cut(s) 113, 228, 608, 713, 797, 1022, 1795
GlaI GCGC 2 cut(s) 921, 1387
GluI GCNGC 9 cut(s) 267, 372, 375, 378, 474, 918, 1110, 1160, 1365
HaeII RGCGCY 1 cut(s) 923
HaeIII GGCC 2 cut(s) 649, 688
HgaI GACGC 1 cut(s) 1271
HhaI GCGC 2 cut(s) 922, 1388
Hin6I GCGC 2 cut(s) 920, 1386
HinP1I GCGC 2 cut(s) 920, 1386
HindIII AAGCTT 1 cut(s) 1428
HinfI GANTC 4 cut(s) 70, 422, 729, 1569
HphI GGTGA 2 cut(s) 104, 785
Hpy166II GTNNAC 6 cut(s) 6, 8, 286, 391, 728, 1376
Hpy188I TCNGA 4 cut(s) 222, 450, 493, 1732
Hpy188III TCNNGA 5 cut(s) 237, 733, 938, 1247, 1650
Hpy8I GTNNAC 6 cut(s) 6, 8, 286, 391, 728, 1376
Hpy99I CGWCG 1 cut(s) 1457
HpyAV CCTTC 4 cut(s) 189, 678, 699, 1297
HpyCH4III ACNGT 4 cut(s) 253, 489, 603, 1351
HpyCH4IV ACGT 3 cut(s) 637, 843, 1783
HpyF10VI GCNNNNNNNGC 5 cut(s) 272, 377, 785, 1109, 1703
HpyF3I CTNAG 3 cut(s) 171, 221, 939
HpySE526I ACGT 3 cut(s) 637, 843, 1783
HspAI GCGC 2 cut(s) 920, 1386
KpnI GGTACC 1 cut(s) 508
Ksp22I TGATCA 3 cut(s) 855, 1072, 1393
KspI CCGCGG 1 cut(s) 378
Kzo9I GATC 8 cut(s) 94, 157, 383, 643, 855, 1072, 1393, 1646
LguI GCTCTTC 2 cut(s) 1155, 1257
LmnI GCTCC 1 cut(s) 272
Lsp1109I GCAGC 7 cut(s) 278, 358, 460, 929, 1121, 1171, 1351
LweI GCATC 4 cut(s) 579, 874, 1006, 1604
MabI ACCWGGT 1 cut(s) 1378
MaeI CTAG 7 cut(s) 113, 228, 608, 713, 797, 1022, 1795
MaeII ACGT 3 cut(s) 637, 843, 1783
MaeIII GTNAC 1 cut(s) 1057
MalI GATC 8 cut(s) 96, 159, 385, 645, 857, 1074, 1395, 1648
MboI GATC 8 cut(s) 94, 157, 383, 643, 855, 1072, 1393, 1646
MfeI CAATTG 1 cut(s) 1683
MflI RGATCY 1 cut(s) 157
MhlI GDGCHC 1 cut(s) 932
MlyI GAGTC 2 cut(s) 723, 1563
MmeI TCCRAC 1 cut(s) 826
Mph1103I ATGCAT 1 cut(s) 1708
MroXI GAANNNNTTC 1 cut(s) 688
MseI TTAA 4 cut(s) 747, 770, 875, 1277
MslI CAYNNNNRTG 1 cut(s) 1002
MspA1I CMGCKG 2 cut(s) 377, 1364
MspR9I CCNGG 4 cut(s) 66, 99, 914, 1380
MunI CAATTG 1 cut(s) 1683
Mva1269I GAATGC 1 cut(s) 755
MvaI CCWGG 4 cut(s) 66, 99, 914, 1380
MvnI CGCG 1 cut(s) 377
MwoI GCNNNNNNNGC 5 cut(s) 272, 377, 785, 1109, 1703
NcoI CCATGG 1 cut(s) 1775
NdeI CATATG 1 cut(s) 1095
NdeII GATC 8 cut(s) 94, 157, 383, 643, 855, 1072, 1393, 1646
NheI GCTAGC 1 cut(s) 227
NlaIV GGNNCC 3 cut(s) 459, 506, 1659
NmuCI GTSAC 1 cut(s) 1057
NsiI ATGCAT 1 cut(s) 1708
NspI RCATGY 2 cut(s) 56, 284
PagI TCATGA 1 cut(s) 732
PasI CCCWGGG 1 cut(s) 65
PceI AGGCCT 1 cut(s) 688
PciI ACATGT 2 cut(s) 52, 280
PciSI GCTCTTC 2 cut(s) 1155, 1257
PctI GAATGC 1 cut(s) 755
PdmI GAANNNNTTC 1 cut(s) 688
PfeI GAWTC 2 cut(s) 70, 422
PflFI GACNNNGTC 1 cut(s) 395
PflMI CCANNNNNTGG 2 cut(s) 65, 104
PfoI TCCNGGA 1 cut(s) 97
PkrI GCNGC 9 cut(s) 268, 373, 376, 379, 475, 919, 1111, 1161, 1366
PleI GAGTC 2 cut(s) 723, 1563
PpsI GAGTC 2 cut(s) 723, 1563
Ppu21I YACGTR 1 cut(s) 638
PscI ACATGT 2 cut(s) 52, 280
Psp124BI GAGCTC 1 cut(s) 932
Psp6I CCWGG 4 cut(s) 64, 97, 912, 1378
PspGI CCWGG 4 cut(s) 64, 97, 912, 1378
PspN4I GGNNCC 3 cut(s) 459, 506, 1659
PstNI CAGNNNCTG 1 cut(s) 104
PsuI RGATCY 1 cut(s) 157
PsyI GACNNNGTC 1 cut(s) 395
PvuII CAGCTG 1 cut(s) 1364
RsaI GTAC 5 cut(s) 7, 57, 506, 512, 640
RsaNI GTAC 5 cut(s) 6, 56, 505, 511, 639
RseI CAYNNNNRTG 1 cut(s) 1002
SacI GAGCTC 1 cut(s) 932
SacII CCGCGG 1 cut(s) 378
SapI GCTCTTC 2 cut(s) 1155, 1257
SaqAI TTAA 4 cut(s) 747, 770, 875, 1277
SatI GCNGC 9 cut(s) 267, 372, 375, 378, 474, 918, 1110, 1160, 1365
Sau3AI GATC 8 cut(s) 94, 157, 383, 643, 855, 1072, 1393, 1646
SchI GAGTC 2 cut(s) 723, 1563
ScrFI CCNGG 4 cut(s) 66, 99, 914, 1380
SduI GDGCHC 1 cut(s) 932
SexAI ACCWGGT 1 cut(s) 1378
SfaNI GCATC 4 cut(s) 579, 874, 1006, 1604
Sfr303I CCGCGG 1 cut(s) 378
SgrBI CCGCGG 1 cut(s) 378
SmiI ATTTAAAT 1 cut(s) 771
SmiMI CAYNNNNRTG 1 cut(s) 1002
SmlI CTYRAG 3 cut(s) 548, 1245, 1538
SmoI CTYRAG 3 cut(s) 548, 1245, 1538
SseBI AGGCCT 1 cut(s) 688
SsiI CCGC 5 cut(s) 84, 375, 377, 663, 1620
SspI AATATT 1 cut(s) 879
SspMI CTAG 7 cut(s) 113, 228, 608, 713, 797, 1022, 1795
SstI GAGCTC 1 cut(s) 932
StuI AGGCCT 1 cut(s) 688
StyD4I CCNGG 4 cut(s) 64, 97, 912, 1378
StyI CCWWGG 2 cut(s) 630, 1775
SwaI ATTTAAAT 1 cut(s) 771
TaaI ACNGT 4 cut(s) 253, 489, 603, 1351
TaiI ACGT 3 cut(s) 640, 846, 1786
TaqI TCGA 5 cut(s) 144, 211, 439, 682, 970
TatI WGTACW 1 cut(s) 5
TauI GCSGC 2 cut(s) 377, 380
TfiI GAWTC 2 cut(s) 70, 422
Tru1I TTAA 4 cut(s) 747, 770, 875, 1277
Tru9I TTAA 4 cut(s) 747, 770, 875, 1277
TscAI CASTG 4 cut(s) 256, 361, 1264, 1315
TseFI GTSAC 1 cut(s) 1057
TseI GCWGC 7 cut(s) 266, 371, 473, 917, 1109, 1159, 1364
Tsp45I GTSAC 1 cut(s) 1057
TspDTI ATGAA 8 cut(s) 176, 248, 749, 975, 1308, 1316, 1492, 1573
TspGWI ACGGA 1 cut(s) 1470
TspRI CASTG 4 cut(s) 256, 361, 1264, 1315
Tth111I GACNNNGTC 1 cut(s) 395
Van91I CCANNNNNTGG 2 cut(s) 65, 104
XapI RAATTY 5 cut(s) 130, 402, 766, 819, 1129
XceI RCATGY 2 cut(s) 56, 284
XcmI CCANNNNNNNNNTGG 2 cut(s) 148, 344
XmiI GTMKAC 3 cut(s) 390, 727, 1375
XmnI GAANNNNTTC 1 cut(s) 688
XspI CTAG 7 cut(s) 113, 228, 608, 713, 797, 1022, 1795
Zsp2I ATGCAT 1 cut(s) 1708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.