FvH4_4g05274

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000176
Physical Location & Seq
Reverse (-)
4552945 .. 4555009
2065 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g05274.t1

Sequence Viewer

Length: 1983 bp
ATGGGCCCAGTTCCGGCACAAGCTGATGACGCCCCACAGATGGAAATTACGAGCAGCATCAAACATAAGCAAACTCTAACCCTCTCCACACCAACTTTGGACATGGCCACTCACATGCGGCCACTATATATCACGGCCGAAGTCGAAGGAAAAATGATCAACAAGGTCATGGTCGACACCGGTGCCGCGGTCACTGTTGTCACAACCCGTACCATGGGACTCCTAGACATCCCGCGCTCGATGATCCAAACCACGTCCTTAACAGTCAAGAACTTCACCGGGCAAGTGTCCAGAACCCTAGGCTTGCTATTCCTACGGGTCAAGGTCGGGCCGGCATCACGGGTTTACACCTTTTTCGTCGCCGAATGCACTTCCTCTTACAGCATGATTCTTGGCCGAGATTGGATCCACAGAAGTTTTTGCGTGCCTTCCTCCATGCACCAAGAACTCATGATTTGGGACGCCGAGAACCGTGAAGCGCTCATCATCAAAGCCGACCCGCGGCCATTCTCTATCTCCGCCAACTTCAACAATGCCCGATATTATACTACAGACTTGGGTCCGTTGGATGTTCAAGACGTCGATAACAAGGGCCGCCCATATGGAATAACGGCCTCCAATCTAACGCGATGGGGACTTACCCAGGCGAAGGACGATTTGCAACGTCCTCACACCATCGCGAGTCCCACACCCCAACGCGAAGCCTACGACAAGGTCGCGACTATGGAATATGTAGATGAAGTGGTGGATTATTCCGATACCAAGGAGGAAGTTATTCACTTAGCTCCCGCCGCCTTGGACGACACACCACCTCAAGTCCGCGACCCCATTTCACAAGTTGACGTAGGGACTGCAGAAAAGCCCTTACTTATTTCTATCAGTGCTAAGTTGGCGGAAGACGAAAAGGAAGCCCTTCTTGCCCTGTTATGCGAGTACAGGGATTGCTTCGCCGACAAATACAAAGATATGCCCGACTTGTCCCCGGACTTGGTCTGCCACCGGTTACCAACTTATCCCGACCGATGTCCAGTACGGCAGGACGGCCGGTTCATGCGAACTGAGACTCAGATCGTAGTCAAGGAAGAGATCGAAAGCATGCATCGATCGGGCATCATTCGCGTGGCAAAGTATAACGAGTGGTTGTCCAATGTTGTCCCCGTTCGCAAGAAAAATGGCAAGATGCGCGTCTGTGTTGATTACCGCGACCTAAACAACGCAACACCGAAGGATATATACCCCATGCTTGTGGCTGACCTACTCATTGATGCCGTCGCGGGGCACGAGGTGTTATCCTTTATGGACGGAATGGCCAGATACCATCAGATTCCCGTCGCTGAGGCAGATCGACACAAGACCGTTTTCCGCTGCCCTGGTTTTGCCGGCGCGTTCGAATATGTTGTCATGCCTTTCGGACTTAAAAACGCAGGGGCAACGTACCAGCGCGCCATGAACCTCATCTTTCATGACATCTTGGGGAAGTTGATCGAAGTATACATCAACGATGTGGTAGTCAAAACCAAAACACGGGCAACCCACGTTGAAGACCTGCGGCAGGTGTTTACACGGATGCGCCGACATAACCTGAAGATGAACCCCTCCAAGTGTGTTTTCTTCGCGGAGGCCGGCGATTTCCTTGGTTTCCTAGTGCATCAAAGAGGCATCGAAATCCCAAAGGACAAGGCACAAGCGGTAATCACCGCTTCGCCCCCCACCACCAAAAAAGAGCTCCAACAGCTGCTCGGGCGAATCAACTTTCTCCGCCGCTTCATATTAAACGCGGCAGGGAAGGTCCAGCCTTTTTCACCCCTCCTCAAGCTCCAGGGGGCAACTGAGTTCGTTTGGGAACCACAACATCAACAGGCCTTCGATCAAATCAAGGACTACCTTACGAACCCACCAGTCCTGATCCCACCTCGCGACGGTGCCCTGCTTAAACTTTATATCGCCGCTGCCGACTTATCCATCGGCGGACTCCTGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

661

Amino Acids

74.13

Weight (kDa)

7.25

Isoelectric Point (pI)

37.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 387 - 547 3.4e-28 Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH_2 PF17919 614 - 659 2.2e-06 RNase H-like domain found in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000176)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21383 FvH4_2g06271 FvH4_2g23662 FvH4_2g35580 FvH4_3g27863 FvH4_4g05274 FvH4_4g18553 FvH4_4g21611 FvH4_5g26253 FvH4_5g34301 FvH4_6g31922 FvH4_7g12391
pyrus_communis pycom03g12260 pycom10g07780 pycom12g20020 pycom15g29760 pycom16g11740 pycom976g00240
rosa_chinensis RchiOBHm_Chr2g0121061 RchiOBHm_Chr5g0033131 RchiOBHm_Chr6g0263211
rosa_multiflora Rmu_co7975030.1_g000001 Rmu_co8030416.1_g000001 Rmu_co8076880.1_g000001 Rmu_co8153002.1_g000001 Rmu_co8164780.1_g000001 Rmu_co8199530.1_g000001 Rmu_co8226177.1_g000002 Rmu_co8305941.1_g000001 Rmu_co8331357.1_g000001 Rmu_co8346103.1_g000001 Rmu_co8386779.1_g000001 Rmu_co8415559.1_g000002 Rmu_co8420415.1_g000002 Rmu_co8517321.1_g000001 Rmu_co8522293.1_g000007 Rmu_sc0000029.1_g000020 Rmu_sc0000147.1_g000010 Rmu_sc0000240.1_g000068 Rmu_sc0000276.1_g000040 Rmu_sc0000322.1_g000020 Rmu_sc0000338.1_g000010 Rmu_sc0000347.1_g000012 Rmu_sc0000461.1_g000002 Rmu_sc0000559.1_g000028 Rmu_sc0000594.1_g000033 Rmu_sc0000613.1_g000009 Rmu_sc0000704.1_g000027 Rmu_sc0000740.1_g000054 Rmu_sc0001051.1_g000021 Rmu_sc0001069.1_g000038 Rmu_sc0001113.1_g000021 Rmu_sc0001250.1_g000030 Rmu_sc0001395.1_g000017 Rmu_sc0001396.1_g000005 Rmu_sc0001738.1_g000043 Rmu_sc0001887.1_g000006 Rmu_sc0001898.1_g000006 Rmu_sc0002014.1_g000019 Rmu_sc0002270.1_g000036 Rmu_sc0002532.1_g000023 Rmu_sc0002638.1_g000047 Rmu_sc0002690.1_g000020 Rmu_sc0002784.1_g000048 Rmu_sc0002882.1_g000024 Rmu_sc0002934.1_g000006 Rmu_sc0002934.1_g000007 Rmu_sc0002938.1_g000028 Rmu_sc0003044.1_g000017 Rmu_sc0003252.1_g000007 Rmu_sc0003737.1_g000023 Rmu_sc0003809.1_g000028 Rmu_sc0003809.1_g000029 Rmu_sc0003936.1_g000019 Rmu_sc0004001.1_g000011 Rmu_sc0004059.1_g000014 Rmu_sc0004101.1_g000022 Rmu_sc0004174.1_g000017 Rmu_sc0004206.1_g000017 Rmu_sc0004206.1_g000018 Rmu_sc0004243.1_g000008 Rmu_sc0004283.1_g000024 Rmu_sc0004549.1_g000015 Rmu_sc0004718.1_g000011 Rmu_sc0004941.1_g000039 Rmu_sc0005112.1_g000017 Rmu_sc0005125.1_g000028 Rmu_sc0005143.1_g000007 Rmu_sc0005147.1_g000009 Rmu_sc0005260.1_g000001 Rmu_sc0005322.1_g000010 Rmu_sc0005454.1_g000008 Rmu_sc0005652.1_g000020 Rmu_sc0005659.1_g000006 Rmu_sc0006059.1_g000057 Rmu_sc0006129.1_g000021 Rmu_sc0006300.1_g000007 Rmu_sc0006561.1_g000011 Rmu_sc0007612.1_g000036 Rmu_sc0007856.1_g000004 Rmu_sc0009191.1_g000019 Rmu_sc0009191.1_g000020 Rmu_sc0009481.1_g000010 Rmu_sc0009901.1_g000002 Rmu_sc0009968.1_g000001 Rmu_sc0010072.1_g000006 Rmu_sc0010090.1_g000011 Rmu_sc0010674.1_g000003 Rmu_sc0011142.1_g000002 Rmu_sc0011514.1_g000004 Rmu_sc0011534.1_g000006 Rmu_sc0011803.1_g000007 Rmu_sc0013216.1_g000009 Rmu_sc0014482.1_g000002 Rmu_sc0015325.1_g000008 Rmu_sc0019673.1_g000001 Rmu_sc0021680.1_g000002 Rmu_sc0022642.1_g000001 Rmu_sc0023778.1_g000005 Rmu_sc0023942.1_g000001 Rmu_sc0024195.1_g000012 Rmu_sc0026069.1_g000002 Rmu_sc0026814.1_g000001 Rmu_sc0028519.1_g000001 Rmu_sc0031008.1_g000002 Rmu_sc0031197.1_g000001 Rmu_sc0031293.1_g000001 Rmu_sc0031294.1_g000001 Rmu_sc0031646.1_g000001 Rmu_sc0034742.1_g000002 Rmu_sc0036436.1_g000001 Rmu_sc0036437.1_g000001 Rmu_sc0042802.1_g000001 Rmu_ssc0000127.1_g000052 Rmu_ssc0000262.1_g000019 Rmu_ssc0000398.1_g000007 Rmu_ssc0000480.1_g000050
rosa_roxburghii Rroxscaffold_174G00435290 Rroxscaffold_177G00434320 Rroxscaffold_178G00437300 Rroxscaffold_180G00433410 Rroxscaffold_1G00010240 Rroxscaffold_1G00020730 Rroxscaffold_1G00059300 Rroxscaffold_2G00077380 Rroxscaffold_2G00077690 Rroxscaffold_3G00218240 Rroxscaffold_3G00223180 Rroxscaffold_3G00223190 Rroxscaffold_3G00255440 Rroxscaffold_3G00255450 Rroxscaffold_3G00259080 Rroxscaffold_3G00274700 Rroxscaffold_4G00306470 Rroxscaffold_4G00316750 Rroxscaffold_4G00323390 Rroxscaffold_4G00331230 Rroxscaffold_5G00339240 Rroxscaffold_5G00339550 Rroxscaffold_5G00378050 Rroxscaffold_6G00392800 Rroxscaffold_7G00163490 Rroxscaffold_7G00199370
rosa_wichuraiana Rw0G015150 Rw1G007650 Rw3G005560 Rw3G028190 Rw5G030760 Rw6G016740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1543
AatII GACGTC 1 cut(s) 582
Acc36I ACCTGC 2 cut(s) 1543, 1554
AccB1I GGYRCC 2 cut(s) 182, 1922
AccI GTMKAC 2 cut(s) 174, 1491
AclWI GGATC 4 cut(s) 238, 400, 413, 1900
AcoI YGGCCR 7 cut(s) 105, 119, 135, 394, 503, 1042, 1308
AcuI CTGAAG 1 cut(s) 1604
AcyI GRCGYC 3 cut(s) 30, 462, 579
AdeI CACNNNGTG 1 cut(s) 1285
AfaI GTAC 4 cut(s) 211, 935, 1032, 1436
AfeI AGCGCT 1 cut(s) 480
AgeI ACCGGT 2 cut(s) 179, 999
AgsI TTSAA 3 cut(s) 529, 575, 1541
AjiI CACGTC 1 cut(s) 255
AjnI CCWGG 4 cut(s) 642, 1369, 1818, 1974
AjuI GAANNNNNNNTTGG 2 cut(s) 1592, 1624
AloI GAACNNNNNNTCC 2 cut(s) 1031, 1063
AluBI AGCT 5 cut(s) 23, 785, 1726, 1735, 1816
AluI AGCT 5 cut(s) 23, 785, 1726, 1735, 1816
Alw21I GWGCWC 1 cut(s) 1728
Alw26I GTCTC 1 cut(s) 1055
AlwI GGATC 4 cut(s) 238, 400, 413, 1900
Ama87I CYCGRG 1 cut(s) 1739
Aor51HI AGCGCT 1 cut(s) 480
ApaI GGGCCC 1 cut(s) 8
ApeKI GCWGC 4 cut(s) 54, 1365, 1735, 1949
ArsI GACNNNNNNTTYG 2 cut(s) 1383, 1415
AsiGI ACCGGT 2 cut(s) 179, 999
Asp700I GAANNNNTTC 2 cut(s) 774, 912
AspA2I CCTAGG 1 cut(s) 298
AspLEI GCGC 7 cut(s) 237, 481, 1185, 1385, 1443, 1445, 1572
AspS9I GGNCC 6 cut(s) 4, 5, 329, 560, 592, 1789
AsuC2I CCSGG 2 cut(s) 280, 983
AsuHPI GGTGA 3 cut(s) 268, 1687, 1794
AsuII TTCGAA 1 cut(s) 1389
AvaI CYCGRG 1 cut(s) 1739
AvaII GGWCC 2 cut(s) 560, 1789
AvrII CCTAGG 1 cut(s) 298
BaeGI GKGCMC 3 cut(s) 8, 1281, 1927
BalI TGGCCA 2 cut(s) 107, 1310
BamHI GGATCC 1 cut(s) 405
BanI GGYRCC 2 cut(s) 182, 1922
BanII GRGCYC 2 cut(s) 8, 1728
BarI GAAGNNNNNNTAC 2 cut(s) 1217, 1249
BauI CACGAG 1 cut(s) 1280
BbsI GAAGAC 2 cut(s) 903, 1548
Bbv12I GWGCWC 1 cut(s) 1728
BbvCI CCTCAGC 1 cut(s) 1335
BbvI GCAGC 4 cut(s) 66, 1352, 1722, 1936
BccI CCATC 5 cut(s) 34, 624, 683, 1326, 1970
BceAI ACGGC 5 cut(s) 150, 627, 1049, 1057, 1253
BcgI CGANNNNNNTGC 2 cut(s) 164, 198
BciT130I CCWGG 4 cut(s) 644, 1371, 1820, 1976
BclI TGATCA 1 cut(s) 156
BcnI CCSGG 2 cut(s) 280, 983
BcoDI GTCTC 1 cut(s) 1055
BfaI CTAG 3 cut(s) 224, 299, 1643
BfmI CTRYAG 2 cut(s) 549, 852
BfoI RGCGCY 1 cut(s) 482
BfuAI ACCTGC 2 cut(s) 1543, 1554
BlnI CCTAGG 1 cut(s) 298
Bme1390I CCNGG 6 cut(s) 280, 644, 983, 1371, 1820, 1976
Bme18I GGWCC 2 cut(s) 560, 1789
BmeT110I CYCGRG 1 cut(s) 1739
BmgBI CACGTC 1 cut(s) 255
BmgT120I GGNCC 6 cut(s) 4, 5, 329, 560, 592, 1789
BmiI GGNNCC 6 cut(s) 6, 184, 407, 561, 1845, 1924
BmrFI CCNGG 6 cut(s) 280, 644, 983, 1371, 1820, 1976
BmsI GCATC 9 cut(s) 66, 344, 1108, 1119, 1170, 1255, 1557, 1657, 1668
BoxI GACNNNNGTC 2 cut(s) 558, 1023
BpiI GAAGAC 2 cut(s) 903, 1548
BpmI CTGGAG 1 cut(s) 1802
Bpu10I CCTNAGC 1 cut(s) 1335
Bpu14I TTCGAA 1 cut(s) 1389
BpuEI CTTGAG 2 cut(s) 798, 1796
BpuMI CCSGG 2 cut(s) 280, 983
Bsa29I ATCGAT 1 cut(s) 1102
BsaHI GRCGYC 3 cut(s) 30, 462, 579
BsaWI WCCGGW 2 cut(s) 179, 999
Bse118I RCCGGY 6 cut(s) 179, 331, 999, 1044, 1379, 1622
Bse1I ACTGG 3 cut(s) 8, 1028, 1898
BseBI CCWGG 4 cut(s) 644, 1371, 1820, 1976
BseCI ATCGAT 1 cut(s) 1102
BseGI GGATG 3 cut(s) 228, 574, 1572
BseMII CTCAG 4 cut(s) 1050, 1079, 1326, 1821
BseNI ACTGG 3 cut(s) 8, 1028, 1898
BsePI GCGCGC 1 cut(s) 1441
BseRI GAGGAG 1 cut(s) 1799
BseSI GKGCMC 3 cut(s) 8, 1281, 1927
BseX3I CGGCCG 2 cut(s) 135, 1042
BseXI GCAGC 4 cut(s) 66, 1352, 1722, 1936
Bsh1285I CGRYCG 4 cut(s) 138, 1021, 1045, 1106
BshNI GGYRCC 2 cut(s) 182, 1922
BshTI ACCGGT 2 cut(s) 179, 999
BshVI ATCGAT 1 cut(s) 1102
BsiEI CGRYCG 4 cut(s) 138, 1021, 1045, 1106
BsiHKAI GWGCWC 1 cut(s) 1728
BsiHKCI CYCGRG 1 cut(s) 1739
BsiSI CCGG 9 cut(s) 14, 180, 279, 332, 983, 1000, 1045, 1380, 1623
BslFI GGGAC 7 cut(s) 231, 473, 648, 669, 862, 964, 1139
BsmAI GTCTC 1 cut(s) 1055
BsmFI GGGAC 7 cut(s) 231, 473, 648, 669, 862, 964, 1139
BsmI GAATGC 1 cut(s) 371
BsoBI CYCGRG 1 cut(s) 1739
Bsp119I TTCGAA 1 cut(s) 1389
Bsp120I GGGCCC 1 cut(s) 4
Bsp1286I GDGCHC 4 cut(s) 8, 1281, 1728, 1927
Bsp19I CCATGG 1 cut(s) 213
Bsp68I TCGCGA 3 cut(s) 680, 719, 1917
BspCNI CTCAG 4 cut(s) 1051, 1078, 1327, 1822
BspDI ATCGAT 1 cut(s) 1102
BspHI TCATGA 2 cut(s) 450, 1462
BspLI GGNNCC 6 cut(s) 6, 184, 407, 561, 1845, 1924
BspMAI CTGCAG 1 cut(s) 856
BspMI ACCTGC 2 cut(s) 1543, 1554
BspPI GGATC 4 cut(s) 238, 400, 413, 1900
BspT104I TTCGAA 1 cut(s) 1389
BspT107I GGYRCC 2 cut(s) 182, 1922
BsrFI RCCGGY 6 cut(s) 179, 331, 999, 1044, 1379, 1622
BsrI ACTGG 3 cut(s) 8, 1028, 1898
BssAI RCCGGY 6 cut(s) 179, 331, 999, 1044, 1379, 1622
BssHII GCGCGC 1 cut(s) 1441
BssNAI GTATAC 1 cut(s) 1492
BssNI GRCGYC 3 cut(s) 30, 462, 579
BssSI CACGAG 1 cut(s) 1280
BssT1I CCWWGG 5 cut(s) 213, 298, 762, 795, 1633
Bst1107I GTATAC 1 cut(s) 1492
Bst2BI CACGAG 1 cut(s) 1280
Bst2UI CCWGG 4 cut(s) 644, 1371, 1820, 1976
Bst4CI ACNGT 5 cut(s) 196, 265, 473, 1357, 1922
Bst6I CTCTTC 1 cut(s) 1077
BstACI GRCGYC 3 cut(s) 30, 462, 579
BstBI TTCGAA 1 cut(s) 1389
BstC8I GCNNGC 7 cut(s) 305, 333, 425, 1097, 1381, 1443, 1624
BstDEI CTNAG 6 cut(s) 781, 885, 1059, 1065, 1335, 1830
BstDSI CCRYGG 3 cut(s) 186, 213, 500
BstEII GGTNACC 1 cut(s) 1002
BstENI CCTNNNNNAGG 1 cut(s) 1550
BstF5I GGATG 3 cut(s) 228, 574, 1572
BstH2I RGCGCY 1 cut(s) 482
BstHHI GCGC 7 cut(s) 237, 481, 1185, 1385, 1443, 1445, 1572
BstMAI GTCTC 1 cut(s) 1055
BstMCI CGRYCG 4 cut(s) 138, 1021, 1045, 1106
BstMWI GCNNNNNNNGC 8 cut(s) 29, 791, 890, 917, 1116, 1182, 1732, 1741
BstNI CCWGG 4 cut(s) 644, 1371, 1820, 1976
BstNSI RCATGY 2 cut(s) 118, 1099
BstPAI GACNNNNGTC 2 cut(s) 558, 1023
BstPI GGTNACC 1 cut(s) 1002
BstSCI CCNGG 6 cut(s) 278, 642, 981, 1369, 1818, 1974
BstSFI CTRYAG 2 cut(s) 549, 852
BstSLI GKGCMC 3 cut(s) 8, 1281, 1927
BstV1I GCAGC 4 cut(s) 66, 1352, 1722, 1936
BstV2I GAAGAC 2 cut(s) 903, 1548
BstX2I RGATCY 1 cut(s) 405
BstXI CCANNNNNNTGG 1 cut(s) 1246
BstYI RGATCY 1 cut(s) 405
BstZ17I GTATAC 1 cut(s) 1492
BstZI CGGCCG 2 cut(s) 135, 1042
Bsu15I ATCGAT 1 cut(s) 1102
BsuTUI ATCGAT 1 cut(s) 1102
BtgI CCRYGG 3 cut(s) 186, 213, 500
BtgZI GCGATG 2 cut(s) 643, 661
BtrI CACGTC 1 cut(s) 255
BtsCI GGATG 3 cut(s) 228, 574, 1572
BtsIMutI CAGTG 2 cut(s) 192, 886
BtuMI TCGCGA 3 cut(s) 680, 719, 1917
BveI ACCTGC 2 cut(s) 1543, 1554
Cac8I GCNNGC 7 cut(s) 305, 333, 425, 1097, 1381, 1443, 1624
CciI TCATGA 2 cut(s) 450, 1462
CfoI GCGC 7 cut(s) 237, 481, 1185, 1385, 1443, 1445, 1572
Cfr10I RCCGGY 6 cut(s) 179, 331, 999, 1044, 1379, 1622
Cfr13I GGNCC 6 cut(s) 4, 5, 329, 560, 592, 1789
Cfr42I CCGCGG 2 cut(s) 189, 503
ClaI ATCGAT 1 cut(s) 1102
CseI GACGC 3 cut(s) 38, 470, 1174
Csp6I GTAC 4 cut(s) 210, 934, 1031, 1435
CspAI ACCGGT 2 cut(s) 179, 999
CviQI GTAC 4 cut(s) 210, 934, 1031, 1435
DdeI CTNAG 6 cut(s) 781, 885, 1059, 1065, 1335, 1830
DraIII CACNNNGTG 1 cut(s) 1285
EaeI YGGCCR 7 cut(s) 105, 119, 135, 394, 503, 1042, 1308
EagI CGGCCG 2 cut(s) 135, 1042
Eam1104I CTCTTC 1 cut(s) 1077
EarI CTCTTC 1 cut(s) 1077
EciI GGCGGA 4 cut(s) 508, 908, 1748, 1983
Ecl136II GAGCTC 1 cut(s) 1726
EclXI CGGCCG 2 cut(s) 135, 1042
Eco130I CCWWGG 5 cut(s) 213, 298, 762, 795, 1633
Eco147I AGGCCT 1 cut(s) 1862
Eco24I GRGCYC 2 cut(s) 8, 1728
Eco47I GGWCC 2 cut(s) 560, 1789
Eco47III AGCGCT 1 cut(s) 480
Eco52I CGGCCG 2 cut(s) 135, 1042
Eco53kI GAGCTC 1 cut(s) 1726
Eco57I CTGAAG 1 cut(s) 1604
Eco88I CYCGRG 1 cut(s) 1739
Eco91I GGTNACC 1 cut(s) 1002
EcoICRI GAGCTC 1 cut(s) 1726
EcoNI CCTNNNNNAGG 1 cut(s) 1550
EcoO65I GGTNACC 1 cut(s) 1002
EcoRII CCWGG 4 cut(s) 642, 1369, 1818, 1974
EcoT14I CCWWGG 5 cut(s) 213, 298, 762, 795, 1633
EcoT22I ATGCAT 1 cut(s) 1101
EcoT38I GRGCYC 2 cut(s) 8, 1728
ErhI CCWWGG 5 cut(s) 213, 298, 762, 795, 1633
FalI AAGNNNNNCTT 2 cut(s) 900, 932
FaqI GGGAC 7 cut(s) 231, 473, 648, 669, 862, 964, 1139
FauI CCCGC 4 cut(s) 240, 507, 796, 1267
FauNDI CATATG 1 cut(s) 601
FbaI TGATCA 1 cut(s) 156
FblI GTMKAC 2 cut(s) 174, 1491
FokI GGATG 3 cut(s) 215, 581, 1579
FriOI GRGCYC 2 cut(s) 8, 1728
FspBI CTAG 3 cut(s) 224, 299, 1643
GlaI GCGC 7 cut(s) 236, 480, 1184, 1384, 1442, 1444, 1571
GsuI CTGGAG 1 cut(s) 1802
HaeII RGCGCY 1 cut(s) 482
HapII CCGG 9 cut(s) 14, 180, 279, 332, 983, 1000, 1045, 1380, 1623
HgaI GACGC 3 cut(s) 38, 470, 1174
HhaI GCGC 7 cut(s) 237, 481, 1185, 1385, 1443, 1445, 1572
Hin1I GRCGYC 3 cut(s) 30, 462, 579
Hin6I GCGC 7 cut(s) 235, 479, 1183, 1383, 1441, 1443, 1570
HinP1I GCGC 7 cut(s) 235, 479, 1183, 1383, 1441, 1443, 1570
HincII GTYRAC 2 cut(s) 175, 841
HindII GTYRAC 2 cut(s) 175, 841
HinfI GANTC 7 cut(s) 219, 388, 682, 1063, 1324, 1746, 1971
HpaII CCGG 9 cut(s) 14, 180, 279, 332, 983, 1000, 1045, 1380, 1623
HphI GGTGA 3 cut(s) 268, 1687, 1794
Hpy166II GTNNAC 5 cut(s) 175, 346, 841, 1492, 1560
Hpy188I TCNGA 4 cut(s) 757, 1068, 1323, 1412
Hpy8I GTNNAC 5 cut(s) 175, 346, 841, 1492, 1560
Hpy99I CGWCG 5 cut(s) 362, 584, 1274, 1334, 1922
HpyAV CCTTC 7 cut(s) 140, 438, 643, 923, 1219, 1780, 1873
HpyCH4III ACNGT 5 cut(s) 196, 265, 473, 1357, 1922
HpyCH4IV ACGT 6 cut(s) 254, 579, 664, 843, 1433, 1536
HpyCH4V TGCA 6 cut(s) 369, 439, 661, 854, 1099, 1648
HpyF10VI GCNNNNNNNGC 8 cut(s) 29, 791, 890, 917, 1116, 1182, 1732, 1741
HpyF3I CTNAG 6 cut(s) 781, 885, 1059, 1065, 1335, 1830
HpySE526I ACGT 6 cut(s) 254, 579, 664, 843, 1433, 1536
Hsp92I GRCGYC 3 cut(s) 30, 462, 579
HspAI GCGC 7 cut(s) 235, 479, 1183, 1383, 1441, 1443, 1570
KroI GCCGGC 3 cut(s) 331, 1379, 1622
KroNI GCCGGC 3 cut(s) 333, 1381, 1624
Ksp22I TGATCA 1 cut(s) 156
KspI CCGCGG 2 cut(s) 189, 503
LmnI GCTCC 3 cut(s) 790, 1731, 1821
Lsp1109I GCAGC 4 cut(s) 66, 1352, 1722, 1936
LweI GCATC 9 cut(s) 66, 344, 1108, 1119, 1170, 1255, 1557, 1657, 1668
MaeI CTAG 3 cut(s) 224, 299, 1643
MaeII ACGT 6 cut(s) 254, 579, 664, 843, 1433, 1536
MaeIII GTNAC 3 cut(s) 190, 199, 1002
MboII GAAGA 5 cut(s) 908, 1094, 1553, 1597, 1603
MflI RGATCY 1 cut(s) 405
MhlI GDGCHC 4 cut(s) 8, 1281, 1728, 1927
MlsI TGGCCA 2 cut(s) 107, 1310
MluCI AATT 1 cut(s) 45
MluNI TGGCCA 2 cut(s) 107, 1310
MlyI GAGTC 4 cut(s) 213, 691, 1057, 1965
MmeI TCCRAC 2 cut(s) 546, 1753
Mox20I TGGCCA 2 cut(s) 107, 1310
Mph1103I ATGCAT 1 cut(s) 1101
MroNI GCCGGC 3 cut(s) 331, 1379, 1622
MroXI GAANNNNTTC 2 cut(s) 774, 912
MscI TGGCCA 2 cut(s) 107, 1310
MseI TTAA 4 cut(s) 260, 1416, 1772, 1932
MslI CAYNNNNRTG 3 cut(s) 113, 1118, 1244
Msp20I TGGCCA 2 cut(s) 107, 1310
MspA1I CMGCKG 5 cut(s) 188, 502, 1365, 1735, 1949
MspI CCGG 9 cut(s) 14, 180, 279, 332, 983, 1000, 1045, 1380, 1623
MspR9I CCNGG 6 cut(s) 280, 644, 983, 1371, 1820, 1976
Mva1269I GAATGC 1 cut(s) 371
MvaI CCWGG 4 cut(s) 644, 1371, 1820, 1976
MwoI GCNNNNNNNGC 8 cut(s) 29, 791, 890, 917, 1116, 1182, 1732, 1741
NaeI GCCGGC 3 cut(s) 333, 1381, 1624
NciI CCSGG 2 cut(s) 280, 983
NcoI CCATGG 1 cut(s) 213
NdeI CATATG 1 cut(s) 601
NgoMIV GCCGGC 3 cut(s) 331, 1379, 1622
NlaIV GGNNCC 6 cut(s) 6, 184, 407, 561, 1845, 1924
NmeAIII GCCGAG 2 cut(s) 422, 490
NmuCI GTSAC 2 cut(s) 190, 199
NruI TCGCGA 3 cut(s) 680, 719, 1917
NsiI ATGCAT 1 cut(s) 1101
NspI RCATGY 2 cut(s) 118, 1099
NspV TTCGAA 1 cut(s) 1389
PaeI GCATGC 1 cut(s) 1099
PagI TCATGA 2 cut(s) 450, 1462
PaqCI CACCTGC 1 cut(s) 1543
PauI GCGCGC 1 cut(s) 1441
PceI AGGCCT 1 cut(s) 1862
PcsI WCGNNNNNNNCGW 3 cut(s) 1278, 1570, 1950
PctI GAATGC 1 cut(s) 371
PdiI GCCGGC 3 cut(s) 333, 1381, 1624
PdmI GAANNNNTTC 2 cut(s) 774, 912
PfeI GAWTC 3 cut(s) 388, 1324, 1746
PflFI GACNNNGTC 2 cut(s) 713, 989
PinAI ACCGGT 2 cut(s) 179, 999
Ple19I CGATCG 1 cut(s) 1106
PleI GAGTC 4 cut(s) 213, 690, 1057, 1965
PpsI GAGTC 4 cut(s) 213, 690, 1057, 1965
PshAI GACNNNNGTC 2 cut(s) 558, 1023
Psp124BI GAGCTC 1 cut(s) 1728
Psp6I CCWGG 4 cut(s) 642, 1369, 1818, 1974
PspEI GGTNACC 1 cut(s) 1002
PspGI CCWGG 4 cut(s) 642, 1369, 1818, 1974
PspN4I GGNNCC 6 cut(s) 6, 184, 407, 561, 1845, 1924
PspOMI GGGCCC 1 cut(s) 4
PspPI GGNCC 6 cut(s) 4, 5, 329, 560, 592, 1789
PstI CTGCAG 1 cut(s) 856
PsuI RGATCY 1 cut(s) 405
PsyI GACNNNGTC 2 cut(s) 713, 989
PteI GCGCGC 1 cut(s) 1441
PvuI CGATCG 1 cut(s) 1106
PvuII CAGCTG 1 cut(s) 1735
RruI TCGCGA 3 cut(s) 680, 719, 1917
RsaI GTAC 4 cut(s) 211, 935, 1032, 1436
RsaNI GTAC 4 cut(s) 210, 934, 1031, 1435
RseI CAYNNNNRTG 3 cut(s) 113, 1118, 1244
SacI GAGCTC 1 cut(s) 1728
SacII CCGCGG 2 cut(s) 189, 503
SalI GTCGAC 1 cut(s) 173
SaqAI TTAA 4 cut(s) 260, 1416, 1772, 1932
Sau96I GGNCC 6 cut(s) 4, 5, 329, 560, 592, 1789
SchI GAGTC 4 cut(s) 213, 691, 1057, 1965
ScrFI CCNGG 6 cut(s) 280, 644, 983, 1371, 1820, 1976
SduI GDGCHC 4 cut(s) 8, 1281, 1728, 1927
SfaNI GCATC 9 cut(s) 66, 344, 1108, 1119, 1170, 1255, 1557, 1657, 1668
SfcI CTRYAG 2 cut(s) 549, 852
Sfr303I CCGCGG 2 cut(s) 189, 503
SfuI TTCGAA 1 cut(s) 1389
SgrAI CRCCGGYG 1 cut(s) 179
SgrBI CCGCGG 2 cut(s) 189, 503
SinI GGWCC 2 cut(s) 560, 1789
SmiMI CAYNNNNRTG 3 cut(s) 113, 1118, 1244
SmlI CTYRAG 2 cut(s) 813, 1811
SmoI CTYRAG 2 cut(s) 813, 1811
SphI GCATGC 1 cut(s) 1099
Sse9I AATT 1 cut(s) 45
SseBI AGGCCT 1 cut(s) 1862
SspMI CTAG 3 cut(s) 224, 299, 1643
SstI GAGCTC 1 cut(s) 1728
StuI AGGCCT 1 cut(s) 1862
StyD4I CCNGG 6 cut(s) 278, 642, 981, 1369, 1818, 1974
StyI CCWWGG 5 cut(s) 213, 298, 762, 795, 1633
TaaI ACNGT 5 cut(s) 196, 265, 473, 1357, 1922
TaiI ACGT 6 cut(s) 257, 582, 667, 846, 1436, 1539
TaqII GACCGA 1 cut(s) 1035
TasI AATT 1 cut(s) 45
TatI WGTACW 1 cut(s) 933
TauI GCSGC 9 cut(s) 121, 188, 505, 597, 794, 1552, 1764, 1781, 1949
TfiI GAWTC 3 cut(s) 388, 1324, 1746
Tru1I TTAA 4 cut(s) 260, 1416, 1772, 1932
Tru9I TTAA 4 cut(s) 260, 1416, 1772, 1932
TscAI CASTG 2 cut(s) 199, 886
TseFI GTSAC 2 cut(s) 190, 199
TseI GCWGC 4 cut(s) 54, 1365, 1735, 1949
Tsp45I GTSAC 2 cut(s) 190, 199
TspDTI ATGAA 6 cut(s) 753, 1039, 1451, 1463, 1604, 1756
TspGWI ACGGA 3 cut(s) 552, 1317, 1579
TspRI CASTG 2 cut(s) 199, 886
Tth111I GACNNNGTC 2 cut(s) 713, 989
VpaK11BI GGWCC 2 cut(s) 560, 1789
XagI CCTNNNNNAGG 1 cut(s) 1550
XceI RCATGY 2 cut(s) 118, 1099
XcmI CCANNNNNNNNNTGG 1 cut(s) 94
XmaJI CCTAGG 1 cut(s) 298
XmiI GTMKAC 2 cut(s) 174, 1491
XmnI GAANNNNTTC 2 cut(s) 774, 912
XspI CTAG 3 cut(s) 224, 299, 1643
ZraI GACGTC 1 cut(s) 580
Zsp2I ATGCAT 1 cut(s) 1101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.