FvH4_5g26253

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000176
Physical Location & Seq
Reverse (-)
17755838 .. 17756799
962 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g26253.t1

Sequence Viewer

Length: 789 bp
ATGGATAGTGCCTTCTCAGAGGAGGCACGCGAGGCTGGCGAGTCCTTGTGCGAAAGACTACTTTCGTATTGGGCAGAACGCGAAGCCTACGACAAGGTCGCGACTATGGAATATGTCGACGAAGTGGTGGATTATACCGATACCAAGGAGGAAGTTATTCAGTTAGCTCCCGCTGCCTTGGACGATACACCACCTCAAGTCCGCGACCCCATTTCACAAGTTGACGTAGGGACTGCAGACAAGCCCTTACTTATTTCCATCAGTGCTATGCTGGCGGAGGACGAAAAGGAAGACCTTCTTGCCCTATTATGTGAGTACAGGGATTGCTTTGCCGACAAGTACGAGGATATGCCTGGCTTATCCCCGAATTTGGTCTGTCACCGGTTGCCAACTTATCCACACCGACTTTTAGTGAGGCAAGACGGCCGATTCATGCGAACTGAGACTCAGATCGTAGTCAAAGAAGAAATCAAAAACATGCACCAATCGGGTATCATTCGTGTAGCAAAGTATAACGAGTGGCTATCCAACGTTGTCCCCGTTCGCAAGAAAAATGGCAAGATGCGTGTCTGTGTTGATTACTGTGACCTCAACAACGCAACGCCCAAGGATATCTACCCCATGCCTGTAGCTGACCTCCTCATTGACGCAGCCGCGGGGCATGAAGTGTTGTCTTTCATGGACGGAACGGCCGGGTACCACCAGATTCCCGTCGCTGAAGAAGATCGACACAAGACGGCTTTCCGCTGTCCCGGTTTTGCCGGTGTTTACACAGATGCGCCTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

263

Amino Acids

29.51

Weight (kDa)

4.67

Isoelectric Point (pI)

33.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 181 - 251 6.2e-08 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000176)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21383 FvH4_2g06271 FvH4_2g23662 FvH4_2g35580 FvH4_3g27863 FvH4_4g05274 FvH4_4g18553 FvH4_4g21611 FvH4_5g26253 FvH4_5g34301 FvH4_6g31922 FvH4_7g12391
pyrus_communis pycom03g12260 pycom10g07780 pycom12g20020 pycom15g29760 pycom16g11740 pycom976g00240
rosa_chinensis RchiOBHm_Chr2g0121061 RchiOBHm_Chr5g0033131 RchiOBHm_Chr6g0263211
rosa_multiflora Rmu_co7975030.1_g000001 Rmu_co8030416.1_g000001 Rmu_co8076880.1_g000001 Rmu_co8153002.1_g000001 Rmu_co8164780.1_g000001 Rmu_co8199530.1_g000001 Rmu_co8226177.1_g000002 Rmu_co8305941.1_g000001 Rmu_co8331357.1_g000001 Rmu_co8346103.1_g000001 Rmu_co8386779.1_g000001 Rmu_co8415559.1_g000002 Rmu_co8420415.1_g000002 Rmu_co8517321.1_g000001 Rmu_co8522293.1_g000007 Rmu_sc0000029.1_g000020 Rmu_sc0000147.1_g000010 Rmu_sc0000240.1_g000068 Rmu_sc0000276.1_g000040 Rmu_sc0000322.1_g000020 Rmu_sc0000338.1_g000010 Rmu_sc0000347.1_g000012 Rmu_sc0000461.1_g000002 Rmu_sc0000559.1_g000028 Rmu_sc0000594.1_g000033 Rmu_sc0000613.1_g000009 Rmu_sc0000704.1_g000027 Rmu_sc0000740.1_g000054 Rmu_sc0001051.1_g000021 Rmu_sc0001069.1_g000038 Rmu_sc0001113.1_g000021 Rmu_sc0001250.1_g000030 Rmu_sc0001395.1_g000017 Rmu_sc0001396.1_g000005 Rmu_sc0001738.1_g000043 Rmu_sc0001887.1_g000006 Rmu_sc0001898.1_g000006 Rmu_sc0002014.1_g000019 Rmu_sc0002270.1_g000036 Rmu_sc0002532.1_g000023 Rmu_sc0002638.1_g000047 Rmu_sc0002690.1_g000020 Rmu_sc0002784.1_g000048 Rmu_sc0002882.1_g000024 Rmu_sc0002934.1_g000006 Rmu_sc0002934.1_g000007 Rmu_sc0002938.1_g000028 Rmu_sc0003044.1_g000017 Rmu_sc0003252.1_g000007 Rmu_sc0003737.1_g000023 Rmu_sc0003809.1_g000028 Rmu_sc0003809.1_g000029 Rmu_sc0003936.1_g000019 Rmu_sc0004001.1_g000011 Rmu_sc0004059.1_g000014 Rmu_sc0004101.1_g000022 Rmu_sc0004174.1_g000017 Rmu_sc0004206.1_g000017 Rmu_sc0004206.1_g000018 Rmu_sc0004243.1_g000008 Rmu_sc0004283.1_g000024 Rmu_sc0004549.1_g000015 Rmu_sc0004718.1_g000011 Rmu_sc0004941.1_g000039 Rmu_sc0005112.1_g000017 Rmu_sc0005125.1_g000028 Rmu_sc0005143.1_g000007 Rmu_sc0005147.1_g000009 Rmu_sc0005260.1_g000001 Rmu_sc0005322.1_g000010 Rmu_sc0005454.1_g000008 Rmu_sc0005652.1_g000020 Rmu_sc0005659.1_g000006 Rmu_sc0006059.1_g000057 Rmu_sc0006129.1_g000021 Rmu_sc0006300.1_g000007 Rmu_sc0006561.1_g000011 Rmu_sc0007612.1_g000036 Rmu_sc0007856.1_g000004 Rmu_sc0009191.1_g000019 Rmu_sc0009191.1_g000020 Rmu_sc0009481.1_g000010 Rmu_sc0009901.1_g000002 Rmu_sc0009968.1_g000001 Rmu_sc0010072.1_g000006 Rmu_sc0010090.1_g000011 Rmu_sc0010674.1_g000003 Rmu_sc0011142.1_g000002 Rmu_sc0011514.1_g000004 Rmu_sc0011534.1_g000006 Rmu_sc0011803.1_g000007 Rmu_sc0013216.1_g000009 Rmu_sc0014482.1_g000002 Rmu_sc0015325.1_g000008 Rmu_sc0019673.1_g000001 Rmu_sc0021680.1_g000002 Rmu_sc0022642.1_g000001 Rmu_sc0023778.1_g000005 Rmu_sc0023942.1_g000001 Rmu_sc0024195.1_g000012 Rmu_sc0026069.1_g000002 Rmu_sc0026814.1_g000001 Rmu_sc0028519.1_g000001 Rmu_sc0031008.1_g000002 Rmu_sc0031197.1_g000001 Rmu_sc0031293.1_g000001 Rmu_sc0031294.1_g000001 Rmu_sc0031646.1_g000001 Rmu_sc0034742.1_g000002 Rmu_sc0036436.1_g000001 Rmu_sc0036437.1_g000001 Rmu_sc0042802.1_g000001 Rmu_ssc0000127.1_g000052 Rmu_ssc0000262.1_g000019 Rmu_ssc0000398.1_g000007 Rmu_ssc0000480.1_g000050
rosa_roxburghii Rroxscaffold_174G00435290 Rroxscaffold_177G00434320 Rroxscaffold_178G00437300 Rroxscaffold_180G00433410 Rroxscaffold_1G00010240 Rroxscaffold_1G00020730 Rroxscaffold_1G00059300 Rroxscaffold_2G00077380 Rroxscaffold_2G00077690 Rroxscaffold_3G00218240 Rroxscaffold_3G00223180 Rroxscaffold_3G00223190 Rroxscaffold_3G00255440 Rroxscaffold_3G00255450 Rroxscaffold_3G00259080 Rroxscaffold_3G00274700 Rroxscaffold_4G00306470 Rroxscaffold_4G00316750 Rroxscaffold_4G00323390 Rroxscaffold_4G00331230 Rroxscaffold_5G00339240 Rroxscaffold_5G00339550 Rroxscaffold_5G00378050 Rroxscaffold_6G00392800 Rroxscaffold_7G00163490 Rroxscaffold_7G00199370
rosa_wichuraiana Rw0G015150 Rw1G007650 Rw3G005560 Rw3G028190 Rw5G030760 Rw6G016740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 696
AccB1I GGYRCC 1 cut(s) 696
AccI GTMKAC 1 cut(s) 117
AccII CGCG 5 cut(s) 30, 81, 101, 204, 656
AciI CCGC 6 cut(s) 171, 202, 275, 654, 656, 745
AclI AACGTT 1 cut(s) 531
AcoI YGGCCR 2 cut(s) 424, 690
AcsI RAATTY 1 cut(s) 367
AcuI CTGAAG 1 cut(s) 738
AfaI GTAC 3 cut(s) 317, 341, 698
AfiI CCNNNNNNNGG 1 cut(s) 370
AgeI ACCGGT 1 cut(s) 381
AjnI CCWGG 1 cut(s) 352
AluBI AGCT 2 cut(s) 167, 632
AluI AGCT 2 cut(s) 167, 632
Alw26I GTCTC 1 cut(s) 437
AoxI GGCC 2 cut(s) 424, 690
ApeKI GCWGC 2 cut(s) 173, 650
ApoI RAATTY 1 cut(s) 367
AsiGI ACCGGT 1 cut(s) 381
Asp700I GAANNNNTTC 2 cut(s) 156, 294
Asp718I GGTACC 1 cut(s) 696
AspLEI GCGC 1 cut(s) 781
AsuC2I CCSGG 2 cut(s) 694, 753
AsuHPI GGTGA 1 cut(s) 371
BanI GGYRCC 1 cut(s) 696
BbsI GAAGAC 1 cut(s) 297
BbvI GCAGC 2 cut(s) 160, 662
BccI CCATC 1 cut(s) 266
BceAI ACGGC 3 cut(s) 439, 705, 753
BciT130I CCWGG 1 cut(s) 354
BcnI CCSGG 2 cut(s) 694, 753
BcoDI GTCTC 1 cut(s) 437
BfmI CTRYAG 2 cut(s) 234, 627
BisI GCNGC 3 cut(s) 174, 651, 654
BlsI GCNGC 3 cut(s) 175, 652, 655
Bme1390I CCNGG 3 cut(s) 354, 694, 753
BmiI GGNNCC 1 cut(s) 698
BmrFI CCNGG 3 cut(s) 354, 694, 753
BmsI GCATC 2 cut(s) 552, 766
BpiI GAAGAC 1 cut(s) 297
BpuEI CTTGAG 1 cut(s) 180
BpuMI CCSGG 2 cut(s) 694, 753
BsaJI CCNNGG 4 cut(s) 144, 177, 606, 654
BsaWI WCCGGW 1 cut(s) 381
Bsc4I CCNNNNNNNGG 1 cut(s) 370
Bse118I RCCGGY 2 cut(s) 381, 761
BseBI CCWGG 1 cut(s) 354
BseDI CCNNGG 4 cut(s) 144, 177, 606, 654
BseLI CCNNNNNNNGG 1 cut(s) 370
BseMII CTCAG 3 cut(s) 30, 432, 461
BseRI GAGGAG 2 cut(s) 35, 629
BseX3I CGGCCG 2 cut(s) 424, 690
BseXI GCAGC 2 cut(s) 160, 662
Bsh1236I CGCG 5 cut(s) 30, 81, 101, 204, 656
Bsh1285I CGRYCG 2 cut(s) 427, 693
BshFI GGCC 2 cut(s) 426, 692
BshNI GGYRCC 1 cut(s) 696
BshTI ACCGGT 1 cut(s) 381
BsiEI CGRYCG 2 cut(s) 427, 693
BsiSI CCGG 4 cut(s) 382, 693, 753, 762
BslFI GGGAC 3 cut(s) 244, 521, 735
BslI CCNNNNNNNGG 1 cut(s) 370
BsmAI GTCTC 1 cut(s) 437
BsmFI GGGAC 3 cut(s) 244, 521, 735
BsnI GGCC 2 cut(s) 426, 692
Bsp143I GATC 2 cut(s) 450, 724
Bsp68I TCGCGA 1 cut(s) 101
BspACI CCGC 6 cut(s) 171, 202, 275, 654, 656, 745
BspANI GGCC 2 cut(s) 426, 692
BspCNI CTCAG 3 cut(s) 29, 433, 460
BspFNI CGCG 5 cut(s) 30, 81, 101, 204, 656
BspLI GGNNCC 1 cut(s) 698
BspMAI CTGCAG 1 cut(s) 238
BspT107I GGYRCC 1 cut(s) 696
BsrFI RCCGGY 2 cut(s) 381, 761
BssAI RCCGGY 2 cut(s) 381, 761
BssECI CCNNGG 4 cut(s) 144, 177, 606, 654
BssMI GATC 2 cut(s) 450, 724
BssT1I CCWWGG 3 cut(s) 144, 177, 606
Bst2UI CCWGG 1 cut(s) 354
Bst4CI ACNGT 1 cut(s) 584
BstC8I GCNNGC 4 cut(s) 28, 37, 273, 783
BstDEI CTNAG 3 cut(s) 16, 441, 447
BstDSI CCRYGG 1 cut(s) 654
BstFNI CGCG 5 cut(s) 30, 81, 101, 204, 656
BstHHI GCGC 1 cut(s) 781
BstKTI GATC 2 cut(s) 453, 727
BstMAI GTCTC 1 cut(s) 437
BstMBI GATC 2 cut(s) 450, 724
BstMCI CGRYCG 2 cut(s) 427, 693
BstMWI GCNNNNNNNGC 4 cut(s) 32, 36, 173, 272
BstNI CCWGG 1 cut(s) 354
BstNSI RCATGY 1 cut(s) 481
BstSCI CCNGG 3 cut(s) 352, 692, 751
BstSFI CTRYAG 2 cut(s) 234, 627
BstUI CGCG 5 cut(s) 30, 81, 101, 204, 656
BstV1I GCAGC 2 cut(s) 160, 662
BstV2I GAAGAC 1 cut(s) 297
BstZI CGGCCG 2 cut(s) 424, 690
BsuRI GGCC 2 cut(s) 426, 692
BtgI CCRYGG 1 cut(s) 654
BtsIMutI CAGTG 1 cut(s) 268
BtuMI TCGCGA 1 cut(s) 101
Cac8I GCNNGC 4 cut(s) 28, 37, 273, 783
CfoI GCGC 1 cut(s) 781
Cfr10I RCCGGY 2 cut(s) 381, 761
Cfr42I CCGCGG 1 cut(s) 657
CseI GACGC 1 cut(s) 656
Csp6I GTAC 3 cut(s) 316, 340, 697
CspAI ACCGGT 1 cut(s) 381
CviAII CATG 5 cut(s) 433, 478, 622, 662, 679
CviQI GTAC 3 cut(s) 316, 340, 697
DdeI CTNAG 3 cut(s) 16, 441, 447
DpnI GATC 2 cut(s) 452, 726
DpnII GATC 2 cut(s) 450, 724
EaeI YGGCCR 2 cut(s) 424, 690
EagI CGGCCG 2 cut(s) 424, 690
EciI GGCGGA 1 cut(s) 290
EclXI CGGCCG 2 cut(s) 424, 690
Eco130I CCWWGG 3 cut(s) 144, 177, 606
Eco32I GATATC 1 cut(s) 613
Eco52I CGGCCG 2 cut(s) 424, 690
Eco57I CTGAAG 1 cut(s) 738
EcoRII CCWGG 1 cut(s) 352
EcoRV GATATC 1 cut(s) 613
EcoT14I CCWWGG 3 cut(s) 144, 177, 606
ErhI CCWWGG 3 cut(s) 144, 177, 606
FaeI CATG 5 cut(s) 436, 481, 625, 665, 682
FalI AAGNNNNNCTT 2 cut(s) 282, 314
FaqI GGGAC 3 cut(s) 244, 521, 735
FatI CATG 5 cut(s) 432, 477, 621, 661, 678
FauI CCCGC 2 cut(s) 178, 649
FblI GTMKAC 1 cut(s) 117
Fnu4HI GCNGC 3 cut(s) 174, 651, 654
Fsp4HI GCNGC 3 cut(s) 174, 651, 654
GlaI GCGC 1 cut(s) 780
GluI GCNGC 3 cut(s) 174, 651, 654
HaeIII GGCC 2 cut(s) 426, 692
HapII CCGG 4 cut(s) 382, 693, 753, 762
HgaI GACGC 1 cut(s) 656
HhaI GCGC 1 cut(s) 781
Hin1II CATG 5 cut(s) 436, 481, 625, 665, 682
Hin6I GCGC 1 cut(s) 779
HinP1I GCGC 1 cut(s) 779
HincII GTYRAC 2 cut(s) 118, 223
HindII GTYRAC 2 cut(s) 118, 223
HinfI GANTC 4 cut(s) 41, 429, 445, 706
HpaII CCGG 4 cut(s) 382, 693, 753, 762
HphI GGTGA 1 cut(s) 371
Hpy166II GTNNAC 3 cut(s) 118, 223, 769
Hpy188I TCNGA 2 cut(s) 19, 450
Hpy188III TCNNGA 1 cut(s) 100
Hpy8I GTNNAC 3 cut(s) 118, 223, 769
Hpy99I CGWCG 2 cut(s) 122, 716
HpyAV CCTTC 2 cut(s) 22, 305
HpyCH4III ACNGT 1 cut(s) 584
HpyCH4IV ACGT 2 cut(s) 225, 531
HpyCH4V TGCA 3 cut(s) 236, 481, 785
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 36, 173, 272
HpyF3I CTNAG 3 cut(s) 16, 441, 447
HpySE526I ACGT 2 cut(s) 225, 531
Hsp92II CATG 5 cut(s) 436, 481, 625, 665, 682
HspAI GCGC 1 cut(s) 779
KpnI GGTACC 1 cut(s) 700
KspI CCGCGG 1 cut(s) 657
Kzo9I GATC 2 cut(s) 450, 724
LmnI GCTCC 1 cut(s) 172
Lsp1109I GCAGC 2 cut(s) 160, 662
LweI GCATC 2 cut(s) 552, 766
MaeII ACGT 2 cut(s) 225, 531
MaeIII GTNAC 2 cut(s) 377, 584
MalI GATC 2 cut(s) 452, 726
MboI GATC 2 cut(s) 450, 724
MboII GAAGA 4 cut(s) 302, 476, 731, 734
MluCI AATT 1 cut(s) 367
MlyI GAGTC 2 cut(s) 50, 439
MmeI TCCRAC 1 cut(s) 552
MroXI GAANNNNTTC 2 cut(s) 156, 294
MspA1I CMGCKG 3 cut(s) 173, 656, 747
MspI CCGG 4 cut(s) 382, 693, 753, 762
MspR9I CCNGG 3 cut(s) 354, 694, 753
MvaI CCWGG 1 cut(s) 354
MvnI CGCG 5 cut(s) 30, 81, 101, 204, 656
MwoI GCNNNNNNNGC 4 cut(s) 32, 36, 173, 272
NciI CCSGG 2 cut(s) 694, 753
NdeII GATC 2 cut(s) 450, 724
NlaIII CATG 5 cut(s) 436, 481, 625, 665, 682
NlaIV GGNNCC 1 cut(s) 698
NmuCI GTSAC 2 cut(s) 377, 584
NruI TCGCGA 1 cut(s) 101
NspI RCATGY 1 cut(s) 481
PcsI WCGNNNNNNNCGW 1 cut(s) 537
PdmI GAANNNNTTC 2 cut(s) 156, 294
PfeI GAWTC 2 cut(s) 429, 706
PflFI GACNNNGTC 1 cut(s) 95
PinAI ACCGGT 1 cut(s) 381
PkrI GCNGC 3 cut(s) 175, 652, 655
PleI GAGTC 2 cut(s) 49, 439
PpsI GAGTC 2 cut(s) 49, 439
Psp1406I AACGTT 1 cut(s) 531
Psp6I CCWGG 1 cut(s) 352
PspGI CCWGG 1 cut(s) 352
PspN4I GGNNCC 1 cut(s) 698
PstI CTGCAG 1 cut(s) 238
PsyI GACNNNGTC 1 cut(s) 95
RruI TCGCGA 1 cut(s) 101
RsaI GTAC 3 cut(s) 317, 341, 698
RsaNI GTAC 3 cut(s) 316, 340, 697
SacII CCGCGG 1 cut(s) 657
SalI GTCGAC 1 cut(s) 116
SatI GCNGC 3 cut(s) 174, 651, 654
Sau3AI GATC 2 cut(s) 450, 724
SchI GAGTC 2 cut(s) 50, 439
ScrFI CCNGG 3 cut(s) 354, 694, 753
SetI ASST 9 cut(s) 99, 169, 196, 228, 297, 534, 591, 634, 639
SfaNI GCATC 2 cut(s) 552, 766
SfcI CTRYAG 2 cut(s) 234, 627
Sfr303I CCGCGG 1 cut(s) 657
SgrBI CCGCGG 1 cut(s) 657
SmlI CTYRAG 1 cut(s) 195
SmoI CTYRAG 1 cut(s) 195
Sse9I AATT 1 cut(s) 367
SsiI CCGC 6 cut(s) 171, 202, 275, 654, 656, 745
StyD4I CCNGG 3 cut(s) 352, 692, 751
StyI CCWWGG 3 cut(s) 144, 177, 606
TaaI ACNGT 1 cut(s) 584
TaiI ACGT 2 cut(s) 228, 534
TaqI TCGA 2 cut(s) 117, 727
TasI AATT 1 cut(s) 367
TatI WGTACW 1 cut(s) 315
TauI GCSGC 1 cut(s) 656
TfiI GAWTC 2 cut(s) 429, 706
TscAI CASTG 1 cut(s) 268
TseFI GTSAC 2 cut(s) 377, 584
TseI GCWGC 2 cut(s) 173, 650
Tsp45I GTSAC 2 cut(s) 377, 584
TspDTI ATGAA 3 cut(s) 421, 667, 678
TspGWI ACGGA 1 cut(s) 699
TspRI CASTG 1 cut(s) 268
Tth111I GACNNNGTC 1 cut(s) 95
XapI RAATTY 1 cut(s) 367
XceI RCATGY 1 cut(s) 481
XmiI GTMKAC 1 cut(s) 117
XmnI GAANNNNTTC 2 cut(s) 156, 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.