pycom03g12260

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000176
Physical Location & Seq
Reverse (-)
12687032 .. 12689178
2147 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g12260.1

Sequence Viewer

Length: 801 bp
ATGCACCACCAACGGAACAATCGTATGGTACACGTTCTACCAGCTGAATTCCAACCGAGTACCCACCAGTCAAGTTTCTTGGACGGGGACGTAGTTGCCGAGGAAGCCACACAAGTAGACTTCGTCGCAAACGACGACGACCGAGAAAACCAGAGCGGAGATAACCTCAAGGCAGCTTTGGCCAAGCTATTTCCCCGCTCCTCCTCGGTTAATCTCCAACACCTGAAGCCGTTGTACATCACGGCTCACATCGAAGGATTTCCCATTTCTAAAGTATTTGTCGACTGTGGAGCCACAGTCAACATTATGCCAATATCCGTCATGAAGGCTTTACGCCGTTCTGATAATGAACTCATTCCGTCAGGGATCACTATGAGCAGCTTTGTTGGTGACAAATCTCAGACCAAAGGGGTGCTCCCGTTGGAAGTTAGCATTGCCAGTCGTCAACACATGACGGCATTCTTTGTCATTGATTCCAAGACGGATTATAACGCATTACTTGGCCGCGACTGGATCCACCAAACCAGTTGCATTCCTTCATCATTATACCAAGTCCTCATCTTTTGGGATGGCAAATCGGTCGTAGTTCACCCAGCCGATAACCAACCATTTGAAACCAACATGATTCAGGCTCGCTATTATGATGATCACGTCGGCTACATTACCCTCCAAGGCCTTAACGAGGATGGACGGCCGACGAGAATTTCAGTACAAAAAGTGATTGAGGTCGGCGCCGAGACTGTCCAACAGGATTCGGCGAGACTTGGCTTGGCGAACTTGGTTATCAATGCCGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

267

Amino Acids

29.53

Weight (kDa)

5.4

Isoelectric Point (pI)

46.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000176)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21383 FvH4_2g06271 FvH4_2g23662 FvH4_2g35580 FvH4_3g27863 FvH4_4g05274 FvH4_4g18553 FvH4_4g21611 FvH4_5g26253 FvH4_5g34301 FvH4_6g31922 FvH4_7g12391
pyrus_communis pycom03g12260 pycom10g07780 pycom12g20020 pycom15g29760 pycom16g11740 pycom976g00240
rosa_chinensis RchiOBHm_Chr2g0121061 RchiOBHm_Chr5g0033131 RchiOBHm_Chr6g0263211
rosa_multiflora Rmu_co7975030.1_g000001 Rmu_co8030416.1_g000001 Rmu_co8076880.1_g000001 Rmu_co8153002.1_g000001 Rmu_co8164780.1_g000001 Rmu_co8199530.1_g000001 Rmu_co8226177.1_g000002 Rmu_co8305941.1_g000001 Rmu_co8331357.1_g000001 Rmu_co8346103.1_g000001 Rmu_co8386779.1_g000001 Rmu_co8415559.1_g000002 Rmu_co8420415.1_g000002 Rmu_co8517321.1_g000001 Rmu_co8522293.1_g000007 Rmu_sc0000029.1_g000020 Rmu_sc0000147.1_g000010 Rmu_sc0000240.1_g000068 Rmu_sc0000276.1_g000040 Rmu_sc0000322.1_g000020 Rmu_sc0000338.1_g000010 Rmu_sc0000347.1_g000012 Rmu_sc0000461.1_g000002 Rmu_sc0000559.1_g000028 Rmu_sc0000594.1_g000033 Rmu_sc0000613.1_g000009 Rmu_sc0000704.1_g000027 Rmu_sc0000740.1_g000054 Rmu_sc0001051.1_g000021 Rmu_sc0001069.1_g000038 Rmu_sc0001113.1_g000021 Rmu_sc0001250.1_g000030 Rmu_sc0001395.1_g000017 Rmu_sc0001396.1_g000005 Rmu_sc0001738.1_g000043 Rmu_sc0001887.1_g000006 Rmu_sc0001898.1_g000006 Rmu_sc0002014.1_g000019 Rmu_sc0002270.1_g000036 Rmu_sc0002532.1_g000023 Rmu_sc0002638.1_g000047 Rmu_sc0002690.1_g000020 Rmu_sc0002784.1_g000048 Rmu_sc0002882.1_g000024 Rmu_sc0002934.1_g000006 Rmu_sc0002934.1_g000007 Rmu_sc0002938.1_g000028 Rmu_sc0003044.1_g000017 Rmu_sc0003252.1_g000007 Rmu_sc0003737.1_g000023 Rmu_sc0003809.1_g000028 Rmu_sc0003809.1_g000029 Rmu_sc0003936.1_g000019 Rmu_sc0004001.1_g000011 Rmu_sc0004059.1_g000014 Rmu_sc0004101.1_g000022 Rmu_sc0004174.1_g000017 Rmu_sc0004206.1_g000017 Rmu_sc0004206.1_g000018 Rmu_sc0004243.1_g000008 Rmu_sc0004283.1_g000024 Rmu_sc0004549.1_g000015 Rmu_sc0004718.1_g000011 Rmu_sc0004941.1_g000039 Rmu_sc0005112.1_g000017 Rmu_sc0005125.1_g000028 Rmu_sc0005143.1_g000007 Rmu_sc0005147.1_g000009 Rmu_sc0005260.1_g000001 Rmu_sc0005322.1_g000010 Rmu_sc0005454.1_g000008 Rmu_sc0005652.1_g000020 Rmu_sc0005659.1_g000006 Rmu_sc0006059.1_g000057 Rmu_sc0006129.1_g000021 Rmu_sc0006300.1_g000007 Rmu_sc0006561.1_g000011 Rmu_sc0007612.1_g000036 Rmu_sc0007856.1_g000004 Rmu_sc0009191.1_g000019 Rmu_sc0009191.1_g000020 Rmu_sc0009481.1_g000010 Rmu_sc0009901.1_g000002 Rmu_sc0009968.1_g000001 Rmu_sc0010072.1_g000006 Rmu_sc0010090.1_g000011 Rmu_sc0010674.1_g000003 Rmu_sc0011142.1_g000002 Rmu_sc0011514.1_g000004 Rmu_sc0011534.1_g000006 Rmu_sc0011803.1_g000007 Rmu_sc0013216.1_g000009 Rmu_sc0014482.1_g000002 Rmu_sc0015325.1_g000008 Rmu_sc0019673.1_g000001 Rmu_sc0021680.1_g000002 Rmu_sc0022642.1_g000001 Rmu_sc0023778.1_g000005 Rmu_sc0023942.1_g000001 Rmu_sc0024195.1_g000012 Rmu_sc0026069.1_g000002 Rmu_sc0026814.1_g000001 Rmu_sc0028519.1_g000001 Rmu_sc0031008.1_g000002 Rmu_sc0031197.1_g000001 Rmu_sc0031293.1_g000001 Rmu_sc0031294.1_g000001 Rmu_sc0031646.1_g000001 Rmu_sc0034742.1_g000002 Rmu_sc0036436.1_g000001 Rmu_sc0036437.1_g000001 Rmu_sc0042802.1_g000001 Rmu_ssc0000127.1_g000052 Rmu_ssc0000262.1_g000019 Rmu_ssc0000398.1_g000007 Rmu_ssc0000480.1_g000050
rosa_roxburghii Rroxscaffold_174G00435290 Rroxscaffold_177G00434320 Rroxscaffold_178G00437300 Rroxscaffold_180G00433410 Rroxscaffold_1G00010240 Rroxscaffold_1G00020730 Rroxscaffold_1G00059300 Rroxscaffold_2G00077380 Rroxscaffold_2G00077690 Rroxscaffold_3G00218240 Rroxscaffold_3G00223180 Rroxscaffold_3G00223190 Rroxscaffold_3G00255440 Rroxscaffold_3G00255450 Rroxscaffold_3G00259080 Rroxscaffold_3G00274700 Rroxscaffold_4G00306470 Rroxscaffold_4G00316750 Rroxscaffold_4G00323390 Rroxscaffold_4G00331230 Rroxscaffold_5G00339240 Rroxscaffold_5G00339550 Rroxscaffold_5G00378050 Rroxscaffold_6G00392800 Rroxscaffold_7G00163490 Rroxscaffold_7G00199370
rosa_wichuraiana Rw0G015150 Rw1G007650 Rw3G005560 Rw3G028190 Rw5G030760 Rw6G016740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 489
AccB1I GGYRCC 1 cut(s) 731
AccBSI CCGCTC 2 cut(s) 156, 198
AccI GTMKAC 2 cut(s) 117, 282
AccII CGCG 1 cut(s) 507
AciI CCGC 3 cut(s) 156, 196, 505
AclWI GGATC 3 cut(s) 374, 508, 521
AcoI YGGCCR 3 cut(s) 180, 502, 692
AcsI RAATTY 2 cut(s) 47, 702
AcuI CTGAAG 1 cut(s) 245
AcyI GRCGYC 1 cut(s) 732
AfaI GTAC 4 cut(s) 30, 61, 236, 711
AfiI CCNNNNNNNGG 1 cut(s) 682
AflIII ACRYGT 1 cut(s) 31
AgsI TTSAA 1 cut(s) 614
AjiI CACGTC 1 cut(s) 652
AluBI AGCT 4 cut(s) 44, 176, 187, 381
AluI AGCT 4 cut(s) 44, 176, 187, 381
Alw21I GWGCWC 1 cut(s) 417
Alw26I GTCTC 2 cut(s) 731, 754
AlwI GGATC 3 cut(s) 374, 508, 521
AoxI GGCC 4 cut(s) 180, 502, 673, 692
ApeKI GCWGC 2 cut(s) 173, 378
ApoI RAATTY 2 cut(s) 47, 702
Asp700I GAANNNNTTC 2 cut(s) 258, 354
AspLEI GCGC 1 cut(s) 734
AsuHPI GGTGA 2 cut(s) 401, 581
BalI TGGCCA 1 cut(s) 182
BamHI GGATCC 1 cut(s) 513
BanI GGYRCC 1 cut(s) 731
BarI GAAGNNNNNNTAC 2 cut(s) 218, 250
Bbv12I GWGCWC 1 cut(s) 417
BbvI GCAGC 2 cut(s) 185, 390
BccI CCATC 2 cut(s) 563, 680
BceAI ACGGC 5 cut(s) 214, 258, 321, 471, 707
BcgI CGANNNNNNTGC 2 cut(s) 562, 596
BclI TGATCA 1 cut(s) 646
BcoDI GTCTC 2 cut(s) 731, 754
BfoI RGCGCY 1 cut(s) 735
BisI GCNGC 3 cut(s) 174, 379, 505
BlsI GCNGC 3 cut(s) 175, 380, 506
BmgBI CACGTC 1 cut(s) 652
BmiI GGNNCC 3 cut(s) 292, 515, 733
BplI GAGNNNNNCTC 2 cut(s) 150, 182
BpuEI CTTGAG 1 cut(s) 152
BsaHI GRCGYC 1 cut(s) 732
BsaJI CCNNGG 3 cut(s) 99, 204, 670
BsaXI ACNNNNNCTCC 2 cut(s) 282, 312
Bsc4I CCNNNNNNNGG 1 cut(s) 682
Bse1I ACTGG 4 cut(s) 67, 438, 515, 525
Bse3DI GCAATG 1 cut(s) 432
BseDI CCNNGG 3 cut(s) 99, 204, 670
BseGI GGATG 2 cut(s) 574, 691
BseLI CCNNNNNNNGG 1 cut(s) 682
BseMI GCAATG 1 cut(s) 432
BseMII CTCAG 1 cut(s) 413
BseNI ACTGG 4 cut(s) 67, 438, 515, 525
BseRI GAGGAG 2 cut(s) 190, 193
BseX3I CGGCCG 1 cut(s) 692
BseXI GCAGC 2 cut(s) 185, 390
BseYI CCCAGC 1 cut(s) 592
Bsh1236I CGCG 1 cut(s) 507
Bsh1285I CGRYCG 3 cut(s) 142, 582, 695
BshFI GGCC 4 cut(s) 182, 504, 675, 694
BshNI GGYRCC 1 cut(s) 731
BsiEI CGRYCG 3 cut(s) 142, 582, 695
BsiHKAI GWGCWC 1 cut(s) 417
BslFI GGGAC 1 cut(s) 101
BslI CCNNNNNNNGG 1 cut(s) 682
BsmAI GTCTC 2 cut(s) 731, 754
BsmFI GGGAC 1 cut(s) 101
BsmI GAATGC 2 cut(s) 458, 531
BsnI GGCC 4 cut(s) 182, 504, 675, 694
Bsp1286I GDGCHC 1 cut(s) 417
Bsp1407I TGTACA 1 cut(s) 234
Bsp143I GATC 3 cut(s) 366, 513, 646
BspACI CCGC 3 cut(s) 156, 196, 505
BspANI GGCC 4 cut(s) 182, 504, 675, 694
BspCNI CTCAG 1 cut(s) 412
BspFNI CGCG 1 cut(s) 507
BspHI TCATGA 1 cut(s) 321
BspLI GGNNCC 3 cut(s) 292, 515, 733
BspPI GGATC 3 cut(s) 374, 508, 521
BspT107I GGYRCC 1 cut(s) 731
BsrBI CCGCTC 2 cut(s) 156, 198
BsrDI GCAATG 1 cut(s) 432
BsrGI TGTACA 1 cut(s) 234
BsrI ACTGG 4 cut(s) 67, 438, 515, 525
BssECI CCNNGG 3 cut(s) 99, 204, 670
BssMI GATC 3 cut(s) 366, 513, 646
BssNI GRCGYC 1 cut(s) 732
BssT1I CCWWGG 1 cut(s) 670
Bst4CI ACNGT 3 cut(s) 287, 298, 742
BstACI GRCGYC 1 cut(s) 732
BstAUI TGTACA 1 cut(s) 234
BstC8I GCNNGC 1 cut(s) 634
BstDEI CTNAG 1 cut(s) 399
BstENI CCTNNNNNAGG 1 cut(s) 680
BstF5I GGATG 2 cut(s) 574, 691
BstFNI CGCG 1 cut(s) 507
BstH2I RGCGCY 1 cut(s) 735
BstHHI GCGC 1 cut(s) 734
BstKTI GATC 3 cut(s) 369, 516, 649
BstMAI GTCTC 2 cut(s) 731, 754
BstMBI GATC 3 cut(s) 366, 513, 646
BstMCI CGRYCG 3 cut(s) 142, 582, 695
BstMWI GCNNNNNNNGC 2 cut(s) 104, 179
BstUI CGCG 1 cut(s) 507
BstV1I GCAGC 2 cut(s) 185, 390
BstX2I RGATCY 1 cut(s) 513
BstYI RGATCY 1 cut(s) 513
BstZI CGGCCG 1 cut(s) 692
BsuRI GGCC 4 cut(s) 182, 504, 675, 694
BtrI CACGTC 1 cut(s) 652
BtsCI GGATG 2 cut(s) 574, 691
Cac8I GCNNGC 1 cut(s) 634
CciI TCATGA 1 cut(s) 321
CfoI GCGC 1 cut(s) 734
Csp6I GTAC 4 cut(s) 29, 60, 235, 710
CviAII CATG 3 cut(s) 322, 451, 622
CviQI GTAC 4 cut(s) 29, 60, 235, 710
DdeI CTNAG 1 cut(s) 399
DinI GGCGCC 1 cut(s) 733
DpnI GATC 3 cut(s) 368, 515, 648
DpnII GATC 3 cut(s) 366, 513, 646
EaeI YGGCCR 3 cut(s) 180, 502, 692
EagI CGGCCG 1 cut(s) 692
EclXI CGGCCG 1 cut(s) 692
Eco130I CCWWGG 1 cut(s) 670
Eco147I AGGCCT 1 cut(s) 675
Eco52I CGGCCG 1 cut(s) 692
Eco57I CTGAAG 1 cut(s) 245
EcoNI CCTNNNNNAGG 1 cut(s) 680
EcoRI GAATTC 1 cut(s) 47
EcoT14I CCWWGG 1 cut(s) 670
EgeI GGCGCC 1 cut(s) 733
EheI GGCGCC 1 cut(s) 733
ErhI CCWWGG 1 cut(s) 670
FaeI CATG 3 cut(s) 325, 454, 625
FaiI YATR 9 cut(s) 26, 308, 323, 374, 452, 489, 547, 623, 642
FaqI GGGAC 1 cut(s) 101
FatI CATG 3 cut(s) 321, 450, 621
FauI CCCGC 1 cut(s) 203
FbaI TGATCA 1 cut(s) 646
FblI GTMKAC 2 cut(s) 117, 282
Fnu4HI GCNGC 3 cut(s) 174, 379, 505
FokI GGATG 2 cut(s) 581, 698
Fsp4HI GCNGC 3 cut(s) 174, 379, 505
GlaI GCGC 1 cut(s) 733
GluI GCNGC 3 cut(s) 174, 379, 505
GsaI CCCAGC 1 cut(s) 596
HaeII RGCGCY 1 cut(s) 735
HaeIII GGCC 4 cut(s) 182, 504, 675, 694
HhaI GCGC 1 cut(s) 734
Hin1I GRCGYC 1 cut(s) 732
Hin1II CATG 3 cut(s) 325, 454, 625
Hin6I GCGC 1 cut(s) 732
HinP1I GCGC 1 cut(s) 732
HincII GTYRAC 3 cut(s) 283, 301, 446
HindII GTYRAC 3 cut(s) 283, 301, 446
HinfI GANTC 3 cut(s) 473, 625, 752
HphI GGTGA 2 cut(s) 401, 581
Hpy166II GTNNAC 6 cut(s) 31, 118, 283, 301, 446, 589
Hpy188I TCNGA 2 cut(s) 343, 402
Hpy188III TCNNGA 1 cut(s) 322
Hpy8I GTNNAC 6 cut(s) 31, 118, 283, 301, 446, 589
Hpy99I CGWCG 5 cut(s) 128, 137, 140, 656, 700
HpyAV CCTTC 3 cut(s) 248, 319, 546
HpyCH4III ACNGT 3 cut(s) 287, 298, 742
HpyCH4IV ACGT 3 cut(s) 33, 90, 651
HpyCH4V TGCA 2 cut(s) 4, 531
HpyF10VI GCNNNNNNNGC 2 cut(s) 104, 179
HpyF3I CTNAG 1 cut(s) 399
HpySE526I ACGT 3 cut(s) 33, 90, 651
Hsp92I GRCGYC 1 cut(s) 732
Hsp92II CATG 3 cut(s) 325, 454, 625
HspAI GCGC 1 cut(s) 732
KasI GGCGCC 1 cut(s) 731
Ksp22I TGATCA 1 cut(s) 646
Kzo9I GATC 3 cut(s) 366, 513, 646
LmnI GCTCC 3 cut(s) 203, 290, 420
Lsp1109I GCAGC 2 cut(s) 185, 390
MaeII ACGT 3 cut(s) 33, 90, 651
MaeIII GTNAC 1 cut(s) 389
MalI GATC 3 cut(s) 368, 515, 648
MbiI CCGCTC 2 cut(s) 156, 198
MboI GATC 3 cut(s) 366, 513, 646
MflI RGATCY 1 cut(s) 513
MhlI GDGCHC 1 cut(s) 417
MlsI TGGCCA 1 cut(s) 182
MluCI AATT 2 cut(s) 47, 702
MluNI TGGCCA 1 cut(s) 182
Mly113I GGCGCC 1 cut(s) 732
MmeI TCCRAC 4 cut(s) 76, 241, 402, 769
MnlI CCTC 8 cut(s) 94, 176, 211, 214, 566, 676, 677, 718
Mox20I TGGCCA 1 cut(s) 182
MroXI GAANNNNTTC 2 cut(s) 258, 354
MscI TGGCCA 1 cut(s) 182
MseI TTAA 2 cut(s) 210, 678
Msp20I TGGCCA 1 cut(s) 182
MspA1I CMGCKG 1 cut(s) 44
Mva1269I GAATGC 2 cut(s) 458, 531
MvnI CGCG 1 cut(s) 507
MwoI GCNNNNNNNGC 2 cut(s) 104, 179
NarI GGCGCC 1 cut(s) 732
NdeII GATC 3 cut(s) 366, 513, 646
NlaIII CATG 3 cut(s) 325, 454, 625
NlaIV GGNNCC 3 cut(s) 292, 515, 733
NmeAIII GCCGAG 2 cut(s) 124, 760
NmuCI GTSAC 1 cut(s) 389
PagI TCATGA 1 cut(s) 321
PceI AGGCCT 1 cut(s) 675
PcsI WCGNNNNNNNCGW 4 cut(s) 19, 96, 129, 132
PctI GAATGC 2 cut(s) 458, 531
PdmI GAANNNNTTC 2 cut(s) 258, 354
PfeI GAWTC 3 cut(s) 473, 625, 752
PflFI GACNNNGTC 1 cut(s) 122
PkrI GCNGC 3 cut(s) 175, 380, 506
PluTI GGCGCC 1 cut(s) 735
PsiI TTATAA 1 cut(s) 489
PspFI CCCAGC 1 cut(s) 592
PspN4I GGNNCC 3 cut(s) 292, 515, 733
PsuI RGATCY 1 cut(s) 513
PsyI GACNNNGTC 1 cut(s) 122
PvuII CAGCTG 1 cut(s) 44
RsaI GTAC 4 cut(s) 30, 61, 236, 711
RsaNI GTAC 4 cut(s) 29, 60, 235, 710
SalI GTCGAC 1 cut(s) 281
SaqAI TTAA 2 cut(s) 210, 678
SatI GCNGC 3 cut(s) 174, 379, 505
Sau3AI GATC 3 cut(s) 366, 513, 646
SduI GDGCHC 1 cut(s) 417
SfoI GGCGCC 1 cut(s) 733
SmlI CTYRAG 1 cut(s) 167
SmoI CTYRAG 1 cut(s) 167
Sse9I AATT 2 cut(s) 47, 702
SseBI AGGCCT 1 cut(s) 675
SsiI CCGC 3 cut(s) 156, 196, 505
SspDI GGCGCC 1 cut(s) 731
StuI AGGCCT 1 cut(s) 675
StyI CCWWGG 1 cut(s) 670
TaaI ACNGT 3 cut(s) 287, 298, 742
TaiI ACGT 3 cut(s) 36, 93, 654
TaqI TCGA 2 cut(s) 252, 282
TaqII GACCGA 2 cut(s) 156, 568
TasI AATT 2 cut(s) 47, 702
TatI WGTACW 2 cut(s) 234, 709
TauI GCSGC 1 cut(s) 507
TfiI GAWTC 3 cut(s) 473, 625, 752
Tru1I TTAA 2 cut(s) 210, 678
Tru9I TTAA 2 cut(s) 210, 678
TseFI GTSAC 1 cut(s) 389
TseI GCWGC 2 cut(s) 173, 378
Tsp45I GTSAC 1 cut(s) 389
TspDTI ATGAA 3 cut(s) 338, 363, 528
TspGWI ACGGA 4 cut(s) 28, 307, 348, 497
Tth111I GACNNNGTC 1 cut(s) 122
XagI CCTNNNNNAGG 1 cut(s) 680
XapI RAATTY 2 cut(s) 47, 702
XmiI GTMKAC 2 cut(s) 117, 282
XmnI GAANNNNTTC 2 cut(s) 258, 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.