Rmu_sc0004174.1_g000017

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004174.1
OG0000176
Physical Location & Seq
Reverse (-)
83479 .. 84488
1010 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004174.1_g000017.1.cds

Sequence Viewer

Length: 816 bp
atggtatacaagggagaagggcatgtagaaatcatcccttcaaagaagaggaagctccaacgccgttttggagaggcgaagcgaaccttggaagccctagatcaagaatcttcgaggaaaagaagagtactcctccatcacctgcaaagaaagaggcctagcttccggcgtaggctgtttcttgaggaaccagattccaaaccctcagtctttgagagactgaggggtaagggaaacactactgagagtttacagaggagacccaagaagatccagagtattgtggttcatgcttcaaaagacgctcgagcagtggagcagaggcaagacccgccgcgaaaggtccctactctcaaagctctcgagcaaggccagattgtggctcccatggaggaggagtggttggttgcttatgtcgccaagaagttcaacgaagagcctgacttgaacatcccagcggatgaacctaagctggaccttggcaatgacatggaagaagaagatgtggtagataccacctgcaatatggtgaatgccacgcagtggatgcttgagatcacttcaacagcttcgacactagtggaagaggctgtgttcctggagactgaggaccccaatagtagaggcttcttcatgagctttaccaaactaacaccaccaatggtccagcacataaggcatttatacatcacagctgacgtcaatggcgtcaaggtcagcaagatcatggtagacactggtgcagcggtcaatgtcgttactataagaaccatgcacttgcttaattggcagtactgtgaggcattgacagaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

271

Amino Acids

31.18

Weight (kDa)

8.43

Isoelectric Point (pI)

56.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000176)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21383 FvH4_2g06271 FvH4_2g23662 FvH4_2g35580 FvH4_3g27863 FvH4_4g05274 FvH4_4g18553 FvH4_4g21611 FvH4_5g26253 FvH4_5g34301 FvH4_6g31922 FvH4_7g12391
pyrus_communis pycom03g12260 pycom10g07780 pycom12g20020 pycom15g29760 pycom16g11740 pycom976g00240
rosa_chinensis RchiOBHm_Chr2g0121061 RchiOBHm_Chr5g0033131 RchiOBHm_Chr6g0263211
rosa_multiflora Rmu_co7975030.1_g000001 Rmu_co8030416.1_g000001 Rmu_co8076880.1_g000001 Rmu_co8153002.1_g000001 Rmu_co8164780.1_g000001 Rmu_co8199530.1_g000001 Rmu_co8226177.1_g000002 Rmu_co8305941.1_g000001 Rmu_co8331357.1_g000001 Rmu_co8346103.1_g000001 Rmu_co8386779.1_g000001 Rmu_co8415559.1_g000002 Rmu_co8420415.1_g000002 Rmu_co8517321.1_g000001 Rmu_co8522293.1_g000007 Rmu_sc0000029.1_g000020 Rmu_sc0000147.1_g000010 Rmu_sc0000240.1_g000068 Rmu_sc0000276.1_g000040 Rmu_sc0000322.1_g000020 Rmu_sc0000338.1_g000010 Rmu_sc0000347.1_g000012 Rmu_sc0000461.1_g000002 Rmu_sc0000559.1_g000028 Rmu_sc0000594.1_g000033 Rmu_sc0000613.1_g000009 Rmu_sc0000704.1_g000027 Rmu_sc0000740.1_g000054 Rmu_sc0001051.1_g000021 Rmu_sc0001069.1_g000038 Rmu_sc0001113.1_g000021 Rmu_sc0001250.1_g000030 Rmu_sc0001395.1_g000017 Rmu_sc0001396.1_g000005 Rmu_sc0001738.1_g000043 Rmu_sc0001887.1_g000006 Rmu_sc0001898.1_g000006 Rmu_sc0002014.1_g000019 Rmu_sc0002270.1_g000036 Rmu_sc0002532.1_g000023 Rmu_sc0002638.1_g000047 Rmu_sc0002690.1_g000020 Rmu_sc0002784.1_g000048 Rmu_sc0002882.1_g000024 Rmu_sc0002934.1_g000006 Rmu_sc0002934.1_g000007 Rmu_sc0002938.1_g000028 Rmu_sc0003044.1_g000017 Rmu_sc0003252.1_g000007 Rmu_sc0003737.1_g000023 Rmu_sc0003809.1_g000028 Rmu_sc0003809.1_g000029 Rmu_sc0003936.1_g000019 Rmu_sc0004001.1_g000011 Rmu_sc0004059.1_g000014 Rmu_sc0004101.1_g000022 Rmu_sc0004174.1_g000017 Rmu_sc0004206.1_g000017 Rmu_sc0004206.1_g000018 Rmu_sc0004243.1_g000008 Rmu_sc0004283.1_g000024 Rmu_sc0004549.1_g000015 Rmu_sc0004718.1_g000011 Rmu_sc0004941.1_g000039 Rmu_sc0005112.1_g000017 Rmu_sc0005125.1_g000028 Rmu_sc0005143.1_g000007 Rmu_sc0005147.1_g000009 Rmu_sc0005260.1_g000001 Rmu_sc0005322.1_g000010 Rmu_sc0005454.1_g000008 Rmu_sc0005652.1_g000020 Rmu_sc0005659.1_g000006 Rmu_sc0006059.1_g000057 Rmu_sc0006129.1_g000021 Rmu_sc0006300.1_g000007 Rmu_sc0006561.1_g000011 Rmu_sc0007612.1_g000036 Rmu_sc0007856.1_g000004 Rmu_sc0009191.1_g000019 Rmu_sc0009191.1_g000020 Rmu_sc0009481.1_g000010 Rmu_sc0009901.1_g000002 Rmu_sc0009968.1_g000001 Rmu_sc0010072.1_g000006 Rmu_sc0010090.1_g000011 Rmu_sc0010674.1_g000003 Rmu_sc0011142.1_g000002 Rmu_sc0011514.1_g000004 Rmu_sc0011534.1_g000006 Rmu_sc0011803.1_g000007 Rmu_sc0013216.1_g000009 Rmu_sc0014482.1_g000002 Rmu_sc0015325.1_g000008 Rmu_sc0019673.1_g000001 Rmu_sc0021680.1_g000002 Rmu_sc0022642.1_g000001 Rmu_sc0023778.1_g000005 Rmu_sc0023942.1_g000001 Rmu_sc0024195.1_g000012 Rmu_sc0026069.1_g000002 Rmu_sc0026814.1_g000001 Rmu_sc0028519.1_g000001 Rmu_sc0031008.1_g000002 Rmu_sc0031197.1_g000001 Rmu_sc0031293.1_g000001 Rmu_sc0031294.1_g000001 Rmu_sc0031646.1_g000001 Rmu_sc0034742.1_g000002 Rmu_sc0036436.1_g000001 Rmu_sc0036437.1_g000001 Rmu_sc0042802.1_g000001 Rmu_ssc0000127.1_g000052 Rmu_ssc0000262.1_g000019 Rmu_ssc0000398.1_g000007 Rmu_ssc0000480.1_g000050
rosa_roxburghii Rroxscaffold_174G00435290 Rroxscaffold_177G00434320 Rroxscaffold_178G00437300 Rroxscaffold_180G00433410 Rroxscaffold_1G00010240 Rroxscaffold_1G00020730 Rroxscaffold_1G00059300 Rroxscaffold_2G00077380 Rroxscaffold_2G00077690 Rroxscaffold_3G00218240 Rroxscaffold_3G00223180 Rroxscaffold_3G00223190 Rroxscaffold_3G00255440 Rroxscaffold_3G00255450 Rroxscaffold_3G00259080 Rroxscaffold_3G00274700 Rroxscaffold_4G00306470 Rroxscaffold_4G00316750 Rroxscaffold_4G00323390 Rroxscaffold_4G00331230 Rroxscaffold_5G00339240 Rroxscaffold_5G00339550 Rroxscaffold_5G00378050 Rroxscaffold_6G00392800 Rroxscaffold_7G00163490 Rroxscaffold_7G00199370
rosa_wichuraiana Rw0G015150 Rw1G007650 Rw3G005560 Rw3G028190 Rw5G030760 Rw6G016740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 150, 527
AatII GACGTC 1 cut(s) 702
Acc36I ACCTGC 2 cut(s) 150, 527
AccB7I CCANNNNNTGG 2 cut(s) 379, 543
AccI GTMKAC 2 cut(s) 6, 732
AccII CGCG 1 cut(s) 337
AciI CCGC 4 cut(s) 332, 335, 458, 746
AclWI GGATC 1 cut(s) 265
AcyI GRCGYC 2 cut(s) 699, 708
AdeI CACNNNGTG 1 cut(s) 543
AfaI GTAC 2 cut(s) 129, 794
AfiI CCNNNNNNNGG 2 cut(s) 379, 543
AgsI TTSAA 5 cut(s) 42, 297, 430, 448, 564
AhlI ACTAGT 1 cut(s) 577
AjnI CCWGG 1 cut(s) 597
AjuI GAANNNNNNNTTGG 2 cut(s) 71, 103
AluBI AGCT 7 cut(s) 55, 162, 359, 472, 569, 639, 695
AluI AGCT 7 cut(s) 55, 162, 359, 472, 569, 639, 695
Alw26I GTCTC 3 cut(s) 211, 253, 596
AlwI GGATC 1 cut(s) 265
Ama87I CYCGRG 2 cut(s) 306, 362
AoxI GGCC 2 cut(s) 155, 370
ApeKI GCWGC 1 cut(s) 743
ArsI GACNNNNNNTTYG 2 cut(s) 192, 224
AspS9I GGNCC 4 cut(s) 343, 475, 610, 664
AsuHPI GGTGA 2 cut(s) 131, 541
AvaI CYCGRG 2 cut(s) 306, 362
AvaII GGWCC 4 cut(s) 343, 475, 610, 664
BbvI GCAGC 1 cut(s) 755
BccI CCATC 1 cut(s) 144
BceAI ACGGC 1 cut(s) 48
BciT130I CCWGG 1 cut(s) 599
BcoDI GTCTC 3 cut(s) 211, 253, 596
BcuI ACTAGT 1 cut(s) 577
BfaI CTAG 3 cut(s) 98, 159, 578
BfuAI ACCTGC 2 cut(s) 150, 527
BisI GCNGC 2 cut(s) 335, 744
BlsI GCNGC 2 cut(s) 336, 745
BmcAI AGTACT 2 cut(s) 129, 794
Bme1390I CCNGG 1 cut(s) 599
Bme18I GGWCC 4 cut(s) 343, 475, 610, 664
BmeT110I CYCGRG 2 cut(s) 306, 362
BmgT120I GGNCC 4 cut(s) 343, 475, 610, 664
BmiI GGNNCC 4 cut(s) 189, 345, 384, 612
BmrFI CCNGG 1 cut(s) 599
BmsI GCATC 1 cut(s) 537
BplI GAGNNNNNCTC 2 cut(s) 117, 149
BpmI CTGGAG 1 cut(s) 620
Bpu10I CCTNAGC 1 cut(s) 468
BpuEI CTTGAG 2 cut(s) 203, 572
BsaHI GRCGYC 2 cut(s) 699, 708
BsaI GGTCTC 1 cut(s) 253
BsaJI CCNNGG 3 cut(s) 87, 387, 478
BsaXI ACNNNNNCTCC 4 cut(s) 383, 386, 413, 416
Bsc4I CCNNNNNNNGG 2 cut(s) 379, 543
Bse1I ACTGG 1 cut(s) 742
Bse3DI GCAATG 1 cut(s) 490
BseBI CCWGG 1 cut(s) 599
BseDI CCNNGG 3 cut(s) 87, 387, 478
BseGI GGATG 4 cut(s) 33, 450, 466, 552
BseLI CCNNNNNNNGG 2 cut(s) 379, 543
BseMI GCAATG 1 cut(s) 490
BseMII CTCAG 4 cut(s) 212, 219, 234, 597
BseNI ACTGG 1 cut(s) 742
BseRI GAGGAG 4 cut(s) 122, 271, 407, 410
BseXI GCAGC 1 cut(s) 755
BseYI CCCAGC 1 cut(s) 454
BsgI GTGCAG 1 cut(s) 762
Bsh1236I CGCG 1 cut(s) 337
BshFI GGCC 2 cut(s) 157, 372
BsiHKCI CYCGRG 2 cut(s) 306, 362
BsiSI CCGG 1 cut(s) 166
BslFI GGGAC 1 cut(s) 329
BslI CCNNNNNNNGG 2 cut(s) 379, 543
BsmAI GTCTC 3 cut(s) 211, 253, 596
BsmFI GGGAC 1 cut(s) 329
BsmI GAATGC 1 cut(s) 538
BsnI GGCC 2 cut(s) 157, 372
Bso31I GGTCTC 1 cut(s) 253
BsoBI CYCGRG 2 cut(s) 306, 362
Bsp143I GATC 4 cut(s) 100, 270, 555, 723
Bsp19I CCATGG 1 cut(s) 387
BspACI CCGC 4 cut(s) 332, 335, 458, 746
BspANI GGCC 2 cut(s) 157, 372
BspCNI CTCAG 4 cut(s) 213, 218, 235, 598
BspFNI CGCG 1 cut(s) 337
BspHI TCATGA 1 cut(s) 633
BspLI GGNNCC 4 cut(s) 189, 345, 384, 612
BspMI ACCTGC 2 cut(s) 150, 527
BspPI GGATC 1 cut(s) 265
BspQI GCTCTTC 1 cut(s) 429
BspTNI GGTCTC 1 cut(s) 253
BsrDI GCAATG 1 cut(s) 490
BsrI ACTGG 1 cut(s) 742
BssECI CCNNGG 3 cut(s) 87, 387, 478
BssMI GATC 4 cut(s) 100, 270, 555, 723
BssNAI GTATAC 1 cut(s) 7
BssNI GRCGYC 2 cut(s) 699, 708
BssT1I CCWWGG 3 cut(s) 87, 387, 478
Bst1107I GTATAC 1 cut(s) 7
Bst2UI CCWGG 1 cut(s) 599
Bst4CI ACNGT 1 cut(s) 797
Bst6I CTCTTC 4 cut(s) 41, 118, 429, 579
BstACI GRCGYC 2 cut(s) 699, 708
BstAPI GCANNNNNTGC 1 cut(s) 547
BstDEI CTNAG 5 cut(s) 205, 221, 243, 468, 606
BstDSI CCRYGG 1 cut(s) 387
BstF5I GGATG 4 cut(s) 33, 450, 466, 552
BstFNI CGCG 1 cut(s) 337
BstKTI GATC 4 cut(s) 103, 273, 558, 726
BstMAI GTCTC 3 cut(s) 211, 253, 596
BstMBI GATC 4 cut(s) 100, 270, 555, 723
BstMWI GCNNNNNNNGC 5 cut(s) 331, 416, 547, 676, 787
BstNI CCWGG 1 cut(s) 599
BstNSI RCATGY 1 cut(s) 26
BstSCI CCNGG 1 cut(s) 597
BstUI CGCG 1 cut(s) 337
BstV1I GCAGC 1 cut(s) 755
BstX2I RGATCY 1 cut(s) 270
BstYI RGATCY 1 cut(s) 270
BstZ17I GTATAC 1 cut(s) 7
BsuRI GGCC 2 cut(s) 157, 372
BtgI CCRYGG 1 cut(s) 387
BtsCI GGATG 4 cut(s) 33, 450, 466, 552
BtsI GCAGTG 2 cut(s) 318, 548
BtsIMutI CAGTG 3 cut(s) 318, 548, 735
BveI ACCTGC 2 cut(s) 150, 527
CciI TCATGA 1 cut(s) 633
Cfr13I GGNCC 4 cut(s) 343, 475, 610, 664
CseI GACGC 2 cut(s) 311, 697
Csp6I GTAC 2 cut(s) 128, 793
CviAII CATG 7 cut(s) 23, 290, 388, 490, 634, 727, 772
CviQI GTAC 2 cut(s) 128, 793
DdeI CTNAG 5 cut(s) 205, 221, 243, 468, 606
DpnI GATC 4 cut(s) 102, 272, 557, 725
DpnII GATC 4 cut(s) 100, 270, 555, 723
DraIII CACNNNGTG 1 cut(s) 543
Eam1104I CTCTTC 4 cut(s) 41, 118, 429, 579
EarI CTCTTC 4 cut(s) 41, 118, 429, 579
Eco130I CCWWGG 3 cut(s) 87, 387, 478
Eco147I AGGCCT 1 cut(s) 157
Eco31I GGTCTC 1 cut(s) 253
Eco47I GGWCC 4 cut(s) 343, 475, 610, 664
Eco88I CYCGRG 2 cut(s) 306, 362
EcoO109I RGGNCCY 2 cut(s) 343, 610
EcoRII CCWGG 1 cut(s) 597
EcoT14I CCWWGG 3 cut(s) 87, 387, 478
ErhI CCWWGG 3 cut(s) 87, 387, 478
FaeI CATG 7 cut(s) 26, 293, 391, 493, 637, 730, 775
FalI AAGNNNNNCTT 2 cut(s) 71, 103
FaqI GGGAC 1 cut(s) 329
FatI CATG 7 cut(s) 22, 289, 387, 489, 633, 726, 771
FauI CCCGC 1 cut(s) 339
FblI GTMKAC 2 cut(s) 6, 732
Fnu4HI GCNGC 2 cut(s) 335, 744
FokI GGATG 4 cut(s) 20, 437, 473, 559
Fsp4HI GCNGC 2 cut(s) 335, 744
FspBI CTAG 3 cut(s) 98, 159, 578
GluI GCNGC 2 cut(s) 335, 744
GsaI CCCAGC 1 cut(s) 458
GsuI CTGGAG 1 cut(s) 620
HaeIII GGCC 2 cut(s) 157, 372
HapII CCGG 1 cut(s) 166
HgaI GACGC 2 cut(s) 311, 697
Hin1I GRCGYC 2 cut(s) 699, 708
Hin1II CATG 7 cut(s) 26, 293, 391, 493, 637, 730, 775
HinfI GANTC 2 cut(s) 107, 194
HpaII CCGG 1 cut(s) 166
HphI GGTGA 2 cut(s) 131, 541
Hpy166II GTNNAC 3 cut(s) 7, 251, 733
Hpy188III TCNNGA 5 cut(s) 104, 182, 274, 362, 634
Hpy8I GTNNAC 3 cut(s) 7, 251, 733
HpyAV CCTTC 2 cut(s) 11, 48
HpyCH4III ACNGT 1 cut(s) 797
HpyCH4IV ACGT 1 cut(s) 699
HpyCH4V TGCA 4 cut(s) 145, 522, 743, 775
HpyF10VI GCNNNNNNNGC 5 cut(s) 331, 416, 547, 676, 787
HpyF3I CTNAG 5 cut(s) 205, 221, 243, 468, 606
HpySE526I ACGT 1 cut(s) 699
Hsp92I GRCGYC 2 cut(s) 699, 708
Hsp92II CATG 7 cut(s) 26, 293, 391, 493, 637, 730, 775
Kzo9I GATC 4 cut(s) 100, 270, 555, 723
LguI GCTCTTC 1 cut(s) 429
LmnI GCTCC 3 cut(s) 60, 316, 388
Lsp1109I GCAGC 1 cut(s) 755
LweI GCATC 1 cut(s) 537
MaeI CTAG 3 cut(s) 98, 159, 578
MaeII ACGT 1 cut(s) 699
MaeIII GTNAC 1 cut(s) 757
MalI GATC 4 cut(s) 102, 272, 557, 725
MboI GATC 4 cut(s) 100, 270, 555, 723
MflI RGATCY 1 cut(s) 270
MluCI AATT 1 cut(s) 784
MmeI TCCRAC 1 cut(s) 82
MseI TTAA 1 cut(s) 783
MspA1I CMGCKG 3 cut(s) 458, 695, 746
MspI CCGG 1 cut(s) 166
MspR9I CCNGG 1 cut(s) 599
Mva1269I GAATGC 1 cut(s) 538
MvaI CCWGG 1 cut(s) 599
MvnI CGCG 1 cut(s) 337
MwoI GCNNNNNNNGC 5 cut(s) 331, 416, 547, 676, 787
NcoI CCATGG 1 cut(s) 387
NdeII GATC 4 cut(s) 100, 270, 555, 723
NlaIII CATG 7 cut(s) 26, 293, 391, 493, 637, 730, 775
NlaIV GGNNCC 4 cut(s) 189, 345, 384, 612
NspI RCATGY 1 cut(s) 26
PaeR7I CTCGAG 2 cut(s) 306, 362
PagI TCATGA 1 cut(s) 633
PaqCI CACCTGC 2 cut(s) 150, 527
PceI AGGCCT 1 cut(s) 157
PciSI GCTCTTC 1 cut(s) 429
PcsI WCGNNNNNNNCGW 1 cut(s) 705
PctI GAATGC 1 cut(s) 538
PfeI GAWTC 2 cut(s) 107, 194
PflMI CCANNNNNTGG 2 cut(s) 379, 543
PfoI TCCNGGA 1 cut(s) 597
PkrI GCNGC 2 cut(s) 336, 745
PpuMI RGGWCCY 2 cut(s) 343, 610
Psp5II RGGWCCY 2 cut(s) 343, 610
Psp6I CCWGG 1 cut(s) 597
PspFI CCCAGC 1 cut(s) 454
PspGI CCWGG 1 cut(s) 597
PspN4I GGNNCC 4 cut(s) 189, 345, 384, 612
PspPI GGNCC 4 cut(s) 343, 475, 610, 664
PspPPI RGGWCCY 2 cut(s) 343, 610
PspXI VCTCGAGB 1 cut(s) 306
PsuI RGATCY 1 cut(s) 270
PvuII CAGCTG 1 cut(s) 695
RsaI GTAC 2 cut(s) 129, 794
RsaNI GTAC 2 cut(s) 128, 793
SapI GCTCTTC 1 cut(s) 429
SaqAI TTAA 1 cut(s) 783
SatI GCNGC 2 cut(s) 335, 744
Sau3AI GATC 4 cut(s) 100, 270, 555, 723
Sau96I GGNCC 4 cut(s) 343, 475, 610, 664
ScaI AGTACT 2 cut(s) 129, 794
ScrFI CCNGG 1 cut(s) 599
SfaNI GCATC 1 cut(s) 537
Sfr274I CTCGAG 2 cut(s) 306, 362
SinI GGWCC 4 cut(s) 343, 475, 610, 664
SlaI CTCGAG 2 cut(s) 306, 362
SmlI CTYRAG 4 cut(s) 182, 306, 362, 551
SmoI CTYRAG 4 cut(s) 182, 306, 362, 551
SpeI ACTAGT 1 cut(s) 577
Sse9I AATT 1 cut(s) 784
SseBI AGGCCT 1 cut(s) 157
SsiI CCGC 4 cut(s) 332, 335, 458, 746
SspMI CTAG 3 cut(s) 98, 159, 578
StuI AGGCCT 1 cut(s) 157
StyD4I CCNGG 1 cut(s) 597
StyI CCWWGG 3 cut(s) 87, 387, 478
TaaI ACNGT 1 cut(s) 797
TaiI ACGT 1 cut(s) 702
TaqI TCGA 4 cut(s) 113, 307, 363, 572
TasI AATT 1 cut(s) 784
TatI WGTACW 2 cut(s) 127, 792
TauI GCSGC 1 cut(s) 337
TfiI GAWTC 2 cut(s) 107, 194
Tru1I TTAA 1 cut(s) 783
Tru9I TTAA 1 cut(s) 783
TscAI CASTG 3 cut(s) 318, 548, 742
TseI GCWGC 1 cut(s) 743
TspDTI ATGAA 3 cut(s) 278, 477, 622
TspRI CASTG 3 cut(s) 318, 548, 742
Van91I CCANNNNNTGG 2 cut(s) 379, 543
VpaK11BI GGWCC 4 cut(s) 343, 475, 610, 664
XceI RCATGY 1 cut(s) 26
XcmI CCANNNNNNNNNTGG 2 cut(s) 65, 523
XhoI CTCGAG 2 cut(s) 306, 362
XmiI GTMKAC 2 cut(s) 6, 732
XspI CTAG 3 cut(s) 98, 159, 578
ZraI GACGTC 1 cut(s) 700
ZrmI AGTACT 2 cut(s) 129, 794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.