Rroxscaffold_3G00255450

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000176
Physical Location & Seq
Reverse (-)
49719453 .. 49720316
864 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00255450.1

Sequence Viewer

Length: 453 bp
ATGAAGGCGCTAGCTGCTAAGCCTTTAGTGGCTCGTCGTAACTTTCAAAAGCAAGTAAGCTCAACATCGTGGGTGTCTAAGCCCGGCGTCATCCGTAGGATCACCAAAGAAAGGACACACCCCCCGGCTAGGGAGGAAAGTTCTCCAAATAGGCGTCCAAGCATCCGACGCACAGCTGCAGATCACTTCAGCCGCAACAACGCCGATGAAAGAAGCAAAGTGTTCCTTGAGACCGACGATGCTAACAGCAAGTGGTCTCCTTATGAGCTTCACAAGACCCACGCCACCATGGTGCAACACATGCGACCTTTGTATATCACAGCGGAAATTAATGGGACTAAGGTCAGCAAGATAATGGTTGATACCGGCGCGGCCGTGAATGTCATTACTACAAGGACCATGCAGCTTTTGGGAATCAAGAAGGAGAAGATCCGCCTACCTCTCTCACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

150

Amino Acids

17.07

Weight (kDa)

10.83

Isoelectric Point (pI)

40.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
gag-asp_proteas PF13975 106 - 143 1e-05 gag-polyprotein putative aspartyl protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000176)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21383 FvH4_2g06271 FvH4_2g23662 FvH4_2g35580 FvH4_3g27863 FvH4_4g05274 FvH4_4g18553 FvH4_4g21611 FvH4_5g26253 FvH4_5g34301 FvH4_6g31922 FvH4_7g12391
pyrus_communis pycom03g12260 pycom10g07780 pycom12g20020 pycom15g29760 pycom16g11740 pycom976g00240
rosa_chinensis RchiOBHm_Chr2g0121061 RchiOBHm_Chr5g0033131 RchiOBHm_Chr6g0263211
rosa_multiflora Rmu_co7975030.1_g000001 Rmu_co8030416.1_g000001 Rmu_co8076880.1_g000001 Rmu_co8153002.1_g000001 Rmu_co8164780.1_g000001 Rmu_co8199530.1_g000001 Rmu_co8226177.1_g000002 Rmu_co8305941.1_g000001 Rmu_co8331357.1_g000001 Rmu_co8346103.1_g000001 Rmu_co8386779.1_g000001 Rmu_co8415559.1_g000002 Rmu_co8420415.1_g000002 Rmu_co8517321.1_g000001 Rmu_co8522293.1_g000007 Rmu_sc0000029.1_g000020 Rmu_sc0000147.1_g000010 Rmu_sc0000240.1_g000068 Rmu_sc0000276.1_g000040 Rmu_sc0000322.1_g000020 Rmu_sc0000338.1_g000010 Rmu_sc0000347.1_g000012 Rmu_sc0000461.1_g000002 Rmu_sc0000559.1_g000028 Rmu_sc0000594.1_g000033 Rmu_sc0000613.1_g000009 Rmu_sc0000704.1_g000027 Rmu_sc0000740.1_g000054 Rmu_sc0001051.1_g000021 Rmu_sc0001069.1_g000038 Rmu_sc0001113.1_g000021 Rmu_sc0001250.1_g000030 Rmu_sc0001395.1_g000017 Rmu_sc0001396.1_g000005 Rmu_sc0001738.1_g000043 Rmu_sc0001887.1_g000006 Rmu_sc0001898.1_g000006 Rmu_sc0002014.1_g000019 Rmu_sc0002270.1_g000036 Rmu_sc0002532.1_g000023 Rmu_sc0002638.1_g000047 Rmu_sc0002690.1_g000020 Rmu_sc0002784.1_g000048 Rmu_sc0002882.1_g000024 Rmu_sc0002934.1_g000006 Rmu_sc0002934.1_g000007 Rmu_sc0002938.1_g000028 Rmu_sc0003044.1_g000017 Rmu_sc0003252.1_g000007 Rmu_sc0003737.1_g000023 Rmu_sc0003809.1_g000028 Rmu_sc0003809.1_g000029 Rmu_sc0003936.1_g000019 Rmu_sc0004001.1_g000011 Rmu_sc0004059.1_g000014 Rmu_sc0004101.1_g000022 Rmu_sc0004174.1_g000017 Rmu_sc0004206.1_g000017 Rmu_sc0004206.1_g000018 Rmu_sc0004243.1_g000008 Rmu_sc0004283.1_g000024 Rmu_sc0004549.1_g000015 Rmu_sc0004718.1_g000011 Rmu_sc0004941.1_g000039 Rmu_sc0005112.1_g000017 Rmu_sc0005125.1_g000028 Rmu_sc0005143.1_g000007 Rmu_sc0005147.1_g000009 Rmu_sc0005260.1_g000001 Rmu_sc0005322.1_g000010 Rmu_sc0005454.1_g000008 Rmu_sc0005652.1_g000020 Rmu_sc0005659.1_g000006 Rmu_sc0006059.1_g000057 Rmu_sc0006129.1_g000021 Rmu_sc0006300.1_g000007 Rmu_sc0006561.1_g000011 Rmu_sc0007612.1_g000036 Rmu_sc0007856.1_g000004 Rmu_sc0009191.1_g000019 Rmu_sc0009191.1_g000020 Rmu_sc0009481.1_g000010 Rmu_sc0009901.1_g000002 Rmu_sc0009968.1_g000001 Rmu_sc0010072.1_g000006 Rmu_sc0010090.1_g000011 Rmu_sc0010674.1_g000003 Rmu_sc0011142.1_g000002 Rmu_sc0011514.1_g000004 Rmu_sc0011534.1_g000006 Rmu_sc0011803.1_g000007 Rmu_sc0013216.1_g000009 Rmu_sc0014482.1_g000002 Rmu_sc0015325.1_g000008 Rmu_sc0019673.1_g000001 Rmu_sc0021680.1_g000002 Rmu_sc0022642.1_g000001 Rmu_sc0023778.1_g000005 Rmu_sc0023942.1_g000001 Rmu_sc0024195.1_g000012 Rmu_sc0026069.1_g000002 Rmu_sc0026814.1_g000001 Rmu_sc0028519.1_g000001 Rmu_sc0031008.1_g000002 Rmu_sc0031197.1_g000001 Rmu_sc0031293.1_g000001 Rmu_sc0031294.1_g000001 Rmu_sc0031646.1_g000001 Rmu_sc0034742.1_g000002 Rmu_sc0036436.1_g000001 Rmu_sc0036437.1_g000001 Rmu_sc0042802.1_g000001 Rmu_ssc0000127.1_g000052 Rmu_ssc0000262.1_g000019 Rmu_ssc0000398.1_g000007 Rmu_ssc0000480.1_g000050
rosa_roxburghii Rroxscaffold_174G00435290 Rroxscaffold_177G00434320 Rroxscaffold_178G00437300 Rroxscaffold_180G00433410 Rroxscaffold_1G00010240 Rroxscaffold_1G00020730 Rroxscaffold_1G00059300 Rroxscaffold_2G00077380 Rroxscaffold_2G00077690 Rroxscaffold_3G00218240 Rroxscaffold_3G00223180 Rroxscaffold_3G00223190 Rroxscaffold_3G00255440 Rroxscaffold_3G00255450 Rroxscaffold_3G00259080 Rroxscaffold_3G00274700 Rroxscaffold_4G00306470 Rroxscaffold_4G00316750 Rroxscaffold_4G00323390 Rroxscaffold_4G00331230 Rroxscaffold_5G00339240 Rroxscaffold_5G00339550 Rroxscaffold_5G00378050 Rroxscaffold_6G00392800 Rroxscaffold_7G00163490 Rroxscaffold_7G00199370
rosa_wichuraiana Rw0G015150 Rw1G007650 Rw3G005560 Rw3G028190 Rw5G030760 Rw6G016740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 371
AciI CCGC 4 cut(s) 193, 323, 371, 433
AclWI GGATC 2 cut(s) 107, 424
AcoI YGGCCR 1 cut(s) 372
AcuI CTGAAG 1 cut(s) 172
AcyI GRCGYC 2 cut(s) 87, 154
AfiI CCNNNNNNNGG 3 cut(s) 111, 129, 130
AgsI TTSAA 1 cut(s) 47
AleI CACNNNNGTG 1 cut(s) 290
AluBI AGCT 5 cut(s) 14, 60, 176, 268, 406
AluI AGCT 5 cut(s) 14, 60, 176, 268, 406
Alw26I GTCTC 2 cut(s) 224, 261
AlwI GGATC 2 cut(s) 107, 424
AoxI GGCC 1 cut(s) 372
ApeKI GCWGC 3 cut(s) 14, 176, 403
AseI ATTAAT 1 cut(s) 330
AspLEI GCGC 2 cut(s) 10, 371
AspS9I GGNCC 1 cut(s) 396
AsuC2I CCSGG 2 cut(s) 84, 125
AsuHPI GGTGA 1 cut(s) 94
AsuNHI GCTAGC 1 cut(s) 10
AvaII GGWCC 1 cut(s) 396
BbvI GCAGC 2 cut(s) 163, 415
BceAI ACGGC 1 cut(s) 359
BcnI CCSGG 2 cut(s) 84, 125
BcoDI GTCTC 2 cut(s) 224, 261
BfaI CTAG 2 cut(s) 11, 129
BfmI CTRYAG 1 cut(s) 177
BfoI RGCGCY 1 cut(s) 11
BisI GCNGC 5 cut(s) 15, 177, 193, 372, 404
BlpI GCTNAGC 1 cut(s) 18
BlsI GCNGC 5 cut(s) 16, 178, 194, 373, 405
Bme1390I CCNGG 2 cut(s) 84, 125
Bme18I GGWCC 1 cut(s) 396
BmgT120I GGNCC 1 cut(s) 396
BmrFI CCNGG 2 cut(s) 84, 125
BmsI GCATC 2 cut(s) 171, 229
BmtI GCTAGC 1 cut(s) 14
BoxI GACNNNNGTC 1 cut(s) 341
Bpu1102I GCTNAGC 1 cut(s) 18
BpuEI CTTGAG 1 cut(s) 248
BpuMI CCSGG 2 cut(s) 84, 125
BsaHI GRCGYC 2 cut(s) 87, 154
BsaI GGTCTC 2 cut(s) 224, 261
BsaJI CCNNGG 2 cut(s) 123, 288
Bsc4I CCNNNNNNNGG 3 cut(s) 111, 129, 130
Bse118I RCCGGY 1 cut(s) 365
BseDI CCNNGG 2 cut(s) 123, 288
BseGI GGATG 2 cut(s) 90, 162
BseLI CCNNNNNNNGG 3 cut(s) 111, 129, 130
BseX3I CGGCCG 1 cut(s) 372
BseXI GCAGC 2 cut(s) 163, 415
Bsh1236I CGCG 1 cut(s) 371
Bsh1285I CGRYCG 1 cut(s) 375
BshFI GGCC 1 cut(s) 374
BsiEI CGRYCG 1 cut(s) 375
BsiSI CCGG 3 cut(s) 84, 125, 366
BslFI GGGAC 1 cut(s) 349
BslI CCNNNNNNNGG 3 cut(s) 111, 129, 130
BsmAI GTCTC 2 cut(s) 224, 261
BsmFI GGGAC 1 cut(s) 349
BsnI GGCC 1 cut(s) 374
Bso31I GGTCTC 2 cut(s) 224, 261
Bsp143I GATC 3 cut(s) 99, 181, 429
Bsp1720I GCTNAGC 1 cut(s) 18
Bsp19I CCATGG 1 cut(s) 288
BspACI CCGC 4 cut(s) 193, 323, 371, 433
BspANI GGCC 1 cut(s) 374
BspFNI CGCG 1 cut(s) 371
BspMAI CTGCAG 1 cut(s) 181
BspOI GCTAGC 1 cut(s) 14
BspPI GGATC 2 cut(s) 107, 424
BspTNI GGTCTC 2 cut(s) 224, 261
BsrFI RCCGGY 1 cut(s) 365
BssAI RCCGGY 1 cut(s) 365
BssECI CCNNGG 2 cut(s) 123, 288
BssMI GATC 3 cut(s) 99, 181, 429
BssNI GRCGYC 2 cut(s) 87, 154
BssT1I CCWWGG 1 cut(s) 288
BstACI GRCGYC 2 cut(s) 87, 154
BstAPI GCANNNNNTGC 1 cut(s) 301
BstC8I GCNNGC 1 cut(s) 12
BstDEI CTNAG 3 cut(s) 18, 78, 339
BstDSI CCRYGG 1 cut(s) 288
BstF5I GGATG 2 cut(s) 90, 162
BstFNI CGCG 1 cut(s) 371
BstH2I RGCGCY 1 cut(s) 11
BstHHI GCGC 2 cut(s) 10, 371
BstKTI GATC 3 cut(s) 102, 184, 432
BstMAI GTCTC 2 cut(s) 224, 261
BstMBI GATC 3 cut(s) 99, 181, 429
BstMCI CGRYCG 1 cut(s) 375
BstMWI GCNNNNNNNGC 3 cut(s) 14, 168, 301
BstNSI RCATGY 1 cut(s) 304
BstPAI GACNNNNGTC 1 cut(s) 341
BstSCI CCNGG 2 cut(s) 82, 123
BstSFI CTRYAG 1 cut(s) 177
BstUI CGCG 1 cut(s) 371
BstV1I GCAGC 2 cut(s) 163, 415
BstX2I RGATCY 1 cut(s) 429
BstYI RGATCY 1 cut(s) 429
BstZI CGGCCG 1 cut(s) 372
BsuRI GGCC 1 cut(s) 374
BtgI CCRYGG 1 cut(s) 288
BtsCI GGATG 2 cut(s) 90, 162
Cac8I GCNNGC 1 cut(s) 12
CfoI GCGC 2 cut(s) 10, 371
Cfr10I RCCGGY 1 cut(s) 365
Cfr13I GGNCC 1 cut(s) 396
CseI GACGC 3 cut(s) 76, 143, 177
CviAII CATG 3 cut(s) 289, 301, 400
DdeI CTNAG 3 cut(s) 18, 78, 339
DpnI GATC 3 cut(s) 101, 183, 431
DpnII GATC 3 cut(s) 99, 181, 429
EaeI YGGCCR 1 cut(s) 372
EagI CGGCCG 1 cut(s) 372
EciI GGCGGA 1 cut(s) 422
EclXI CGGCCG 1 cut(s) 372
Eco130I CCWWGG 1 cut(s) 288
Eco31I GGTCTC 2 cut(s) 224, 261
Eco47I GGWCC 1 cut(s) 396
Eco52I CGGCCG 1 cut(s) 372
Eco57I CTGAAG 1 cut(s) 172
EcoT14I CCWWGG 1 cut(s) 288
ErhI CCWWGG 1 cut(s) 288
FaeI CATG 3 cut(s) 292, 304, 403
FaiI YATR 5 cut(s) 264, 290, 302, 315, 401
FalI AAGNNNNNCTT 2 cut(s) 210, 242
FaqI GGGAC 1 cut(s) 349
FatI CATG 3 cut(s) 288, 300, 399
Fnu4HI GCNGC 5 cut(s) 15, 177, 193, 372, 404
FokI GGATG 2 cut(s) 77, 149
Fsp4HI GCNGC 5 cut(s) 15, 177, 193, 372, 404
FspBI CTAG 2 cut(s) 11, 129
GlaI GCGC 2 cut(s) 9, 370
GluI GCNGC 5 cut(s) 15, 177, 193, 372, 404
HaeII RGCGCY 1 cut(s) 11
HaeIII GGCC 1 cut(s) 374
HapII CCGG 3 cut(s) 84, 125, 366
HgaI GACGC 3 cut(s) 76, 143, 177
HhaI GCGC 2 cut(s) 10, 371
Hin1I GRCGYC 2 cut(s) 87, 154
Hin1II CATG 3 cut(s) 292, 304, 403
Hin6I GCGC 2 cut(s) 8, 369
HinP1I GCGC 2 cut(s) 8, 369
HinfI GANTC 1 cut(s) 414
HpaII CCGG 3 cut(s) 84, 125, 366
HphI GGTGA 1 cut(s) 94
Hpy188I TCNGA 1 cut(s) 167
Hpy188III TCNNGA 1 cut(s) 418
Hpy99I CGWCG 3 cut(s) 39, 171, 239
HpyAV CCTTC 1 cut(s) 415
HpyCH4V TGCA 3 cut(s) 179, 295, 403
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 168, 301
HpyF3I CTNAG 3 cut(s) 18, 78, 339
Hsp92I GRCGYC 2 cut(s) 87, 154
Hsp92II CATG 3 cut(s) 292, 304, 403
HspAI GCGC 2 cut(s) 8, 369
Kzo9I GATC 3 cut(s) 99, 181, 429
LpnPI CCDG 3 cut(s) 97, 138, 379
Lsp1109I GCAGC 2 cut(s) 163, 415
LweI GCATC 2 cut(s) 171, 229
MaeI CTAG 2 cut(s) 11, 129
MaeIII GTNAC 1 cut(s) 38
MalI GATC 3 cut(s) 101, 183, 431
MboI GATC 3 cut(s) 99, 181, 429
MboII GAAGA 1 cut(s) 439
MflI RGATCY 1 cut(s) 429
MluCI AATT 1 cut(s) 327
MmeI TCCRAC 1 cut(s) 190
MnlI CCTC 2 cut(s) 127, 450
MseI TTAA 1 cut(s) 330
MslI CAYNNNNRTG 1 cut(s) 290
MspA1I CMGCKG 2 cut(s) 176, 323
MspI CCGG 3 cut(s) 84, 125, 366
MspR9I CCNGG 2 cut(s) 84, 125
MvnI CGCG 1 cut(s) 371
MwoI GCNNNNNNNGC 3 cut(s) 14, 168, 301
NciI CCSGG 2 cut(s) 84, 125
NcoI CCATGG 1 cut(s) 288
NdeII GATC 3 cut(s) 99, 181, 429
NheI GCTAGC 1 cut(s) 10
NlaIII CATG 3 cut(s) 292, 304, 403
NspI RCATGY 1 cut(s) 304
OliI CACNNNNGTG 1 cut(s) 290
PfeI GAWTC 1 cut(s) 414
PkrI GCNGC 5 cut(s) 16, 178, 194, 373, 405
PshAI GACNNNNGTC 1 cut(s) 341
PshBI ATTAAT 1 cut(s) 330
PspPI GGNCC 1 cut(s) 396
PstI CTGCAG 1 cut(s) 181
PsuI RGATCY 1 cut(s) 429
PvuII CAGCTG 1 cut(s) 176
RseI CAYNNNNRTG 1 cut(s) 290
SaqAI TTAA 1 cut(s) 330
SatI GCNGC 5 cut(s) 15, 177, 193, 372, 404
Sau3AI GATC 3 cut(s) 99, 181, 429
Sau96I GGNCC 1 cut(s) 396
ScrFI CCNGG 2 cut(s) 84, 125
SetI ASST 8 cut(s) 16, 62, 178, 270, 310, 345, 408, 442
SfaNI GCATC 2 cut(s) 171, 229
SfcI CTRYAG 1 cut(s) 177
SinI GGWCC 1 cut(s) 396
SmiMI CAYNNNNRTG 1 cut(s) 290
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
Sse9I AATT 1 cut(s) 327
SsiI CCGC 4 cut(s) 193, 323, 371, 433
SspMI CTAG 2 cut(s) 11, 129
StyD4I CCNGG 2 cut(s) 82, 123
StyI CCWWGG 1 cut(s) 288
TaqII GACCGA 1 cut(s) 248
TasI AATT 1 cut(s) 327
TauI GCSGC 2 cut(s) 195, 374
TfiI GAWTC 1 cut(s) 414
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TseI GCWGC 3 cut(s) 14, 176, 403
TspDTI ATGAA 2 cut(s) 17, 222
TspGWI ACGGA 1 cut(s) 83
VpaK11BI GGWCC 1 cut(s) 396
VspI ATTAAT 1 cut(s) 330
XceI RCATGY 1 cut(s) 304
XcmI CCANNNNNNNNNTGG 1 cut(s) 406
XspI CTAG 2 cut(s) 11, 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.