FvH4_6g31922

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000176
Physical Location & Seq
Forward (+)
25023266 .. 25025399
2134 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g31922.t1

Sequence Viewer

Length: 1974 bp
ATGGTTCCCCGAGTATCCGCCCTACAACGGTTGAAAAAAGAAGACAACGATAAGGCTCCTCTGGCAACAGACAAACAACCAGCCAAGGACCATCAGCCTTCAACCAAGTCAGTATGGCGTCCGCGGCAACCCGAGGCTCAAGAGCCACCAATAGCCGCACAGGCGCCTTCCAGTCCTAACCTCGCCCAACAAATGGCCCAGCGTTTCCGCGAGAATTCAGAAGACGACGAGGCACATTCAGAACCTCGACTTAACCTAGAGGAAGACCGGGACGAAGGGCAGGCAAAATCATTCACCTGTAATATGATAGAGATCTCAGGATGGGGTGCTGATGAAGGATATAGCAGCGAAGCAGACGCTTTCGCGTTGCTCGAAGGGGAACACGGGATCATTGACACTCCCTTGGTCCCAGTTCCTGAACAATTCTCCTTTCAAGTAGGGATGGTGTTCTTCATTTCGGAAAGATACGCTCTCCCACCAAACACAACCTGCAGTGCGGCGGATATTCCCGTTAGCAGCGAGGATGTCAATAGCATACCCCCGCCATGCAGCAGCGAAATGGGCCCAGTTCCGGCACAAGCCGACGACGCCCCACAGGTGGAAATTACGAGCAGCATCAAACATAAGCAAACTCTAACCTTCTCCACACCAACTTTGGACATGGCCACTCACATGCGGCCACTGTATATCACGGCCGAAGTCGAAGGAAAAATGATCAACAAGGTCATGGTCGACACCAGTGCTGCGGTCAATGTTGTCACAACCCGTACCATGGGACTCCTAGACATCCCGCGCTCGATGATCCAAACCACGTCCTTAACAGTCAAGAACTTCACCGGGCAAGTGTCCAGAACCCTAGGCTTGCTATTCCTACGGGTCAAGGTCGAGCCGGCATCACGGGTTTACACCTTTTTCGTCGCCGAATGCGCTTCCTCTTACAGCATGATTCTTGGCCGAGATTGGATCCACAGAAGTTTTTGCGTACCTTCCTCCATGCACCAAGAACTCATGATTTGGGACGTCGAGAACCGTGAAGCGGTCATCATCAAAGCCGACCCGCGGCCATTCTCTATCTCCGCCAACTTCAACAATGCCCGATATTATACTACAGACTTGAAGGAGGCACGCGAGGCTGGCGAGTCCTTGTGCGAAAGACTCCTTTCGTACTGGGCAGAACGCGAAGCCTACGACAAGGTCGCGACTATGGAATATGTAGATAAAGTGGTGGATTATTCCAATACCAAGGAGGAAGTTATTCACTTAGCTCCCGCCGCCTTGGACGACACACCACCTCAGGTCCGCGACCCCATTTCACAAGTTGACGTAGGGACTGCAGAAAAGCCCTTACTTATTTCTATCAGTGCTAAGTTGGCGGAAGACGAAAAGGAAGCCCTTCTTGCCTTGTTATGCGAGTACAGGGATTGCTTCGCCGACAAATACGAAGATATGCCCGGCTTGTCCCCGGACTTGGTCTACCACCGGTTACCAACTTATCCCGACCGACGTCCAGTACGGCAGGATGGCCGGTTCATGCGAACTGAGACTCAGATCGTAGTCAAGAAAGAAATCGAAAGCATGCATCGATCGGGCATCATTCGCGTGGCAAAGTATAACGAGTGGCTGTCCAATGTTGTCCCCGTTCGCAAGAAAAATGGCAAGATGCGCGTCTGTGTTGATTACCGTGACCTAAACAACGCAACACCGAAGGATATATACCCCATGCCTGTGGCTGACCTACTCATTGATGCCGCCGCGGGACACGAGATGTTATCCTTTATGGACGGAACGGCCGGATACCATCAAATTCTCGTCGCTGAGGAAGATCGACACAAGACCGCTTTCCGCTGCCCTGGTTTTGCCAGCGCGTTCGAATATGTTGTCATGCCTTTCGGACTTAAAAACGCAGGGGCAACGTACCAGCGCGCCATGAACCTCATCTTTCATGACATCTTGGGGAAGTTGATCGAAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

658

Amino Acids

73.63

Weight (kDa)

5.15

Isoelectric Point (pI)

40.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 547 - 652 3e-16 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000176)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21383 FvH4_2g06271 FvH4_2g23662 FvH4_2g35580 FvH4_3g27863 FvH4_4g05274 FvH4_4g18553 FvH4_4g21611 FvH4_5g26253 FvH4_5g34301 FvH4_6g31922 FvH4_7g12391
pyrus_communis pycom03g12260 pycom10g07780 pycom12g20020 pycom15g29760 pycom16g11740 pycom976g00240
rosa_chinensis RchiOBHm_Chr2g0121061 RchiOBHm_Chr5g0033131 RchiOBHm_Chr6g0263211
rosa_multiflora Rmu_co7975030.1_g000001 Rmu_co8030416.1_g000001 Rmu_co8076880.1_g000001 Rmu_co8153002.1_g000001 Rmu_co8164780.1_g000001 Rmu_co8199530.1_g000001 Rmu_co8226177.1_g000002 Rmu_co8305941.1_g000001 Rmu_co8331357.1_g000001 Rmu_co8346103.1_g000001 Rmu_co8386779.1_g000001 Rmu_co8415559.1_g000002 Rmu_co8420415.1_g000002 Rmu_co8517321.1_g000001 Rmu_co8522293.1_g000007 Rmu_sc0000029.1_g000020 Rmu_sc0000147.1_g000010 Rmu_sc0000240.1_g000068 Rmu_sc0000276.1_g000040 Rmu_sc0000322.1_g000020 Rmu_sc0000338.1_g000010 Rmu_sc0000347.1_g000012 Rmu_sc0000461.1_g000002 Rmu_sc0000559.1_g000028 Rmu_sc0000594.1_g000033 Rmu_sc0000613.1_g000009 Rmu_sc0000704.1_g000027 Rmu_sc0000740.1_g000054 Rmu_sc0001051.1_g000021 Rmu_sc0001069.1_g000038 Rmu_sc0001113.1_g000021 Rmu_sc0001250.1_g000030 Rmu_sc0001395.1_g000017 Rmu_sc0001396.1_g000005 Rmu_sc0001738.1_g000043 Rmu_sc0001887.1_g000006 Rmu_sc0001898.1_g000006 Rmu_sc0002014.1_g000019 Rmu_sc0002270.1_g000036 Rmu_sc0002532.1_g000023 Rmu_sc0002638.1_g000047 Rmu_sc0002690.1_g000020 Rmu_sc0002784.1_g000048 Rmu_sc0002882.1_g000024 Rmu_sc0002934.1_g000006 Rmu_sc0002934.1_g000007 Rmu_sc0002938.1_g000028 Rmu_sc0003044.1_g000017 Rmu_sc0003252.1_g000007 Rmu_sc0003737.1_g000023 Rmu_sc0003809.1_g000028 Rmu_sc0003809.1_g000029 Rmu_sc0003936.1_g000019 Rmu_sc0004001.1_g000011 Rmu_sc0004059.1_g000014 Rmu_sc0004101.1_g000022 Rmu_sc0004174.1_g000017 Rmu_sc0004206.1_g000017 Rmu_sc0004206.1_g000018 Rmu_sc0004243.1_g000008 Rmu_sc0004283.1_g000024 Rmu_sc0004549.1_g000015 Rmu_sc0004718.1_g000011 Rmu_sc0004941.1_g000039 Rmu_sc0005112.1_g000017 Rmu_sc0005125.1_g000028 Rmu_sc0005143.1_g000007 Rmu_sc0005147.1_g000009 Rmu_sc0005260.1_g000001 Rmu_sc0005322.1_g000010 Rmu_sc0005454.1_g000008 Rmu_sc0005652.1_g000020 Rmu_sc0005659.1_g000006 Rmu_sc0006059.1_g000057 Rmu_sc0006129.1_g000021 Rmu_sc0006300.1_g000007 Rmu_sc0006561.1_g000011 Rmu_sc0007612.1_g000036 Rmu_sc0007856.1_g000004 Rmu_sc0009191.1_g000019 Rmu_sc0009191.1_g000020 Rmu_sc0009481.1_g000010 Rmu_sc0009901.1_g000002 Rmu_sc0009968.1_g000001 Rmu_sc0010072.1_g000006 Rmu_sc0010090.1_g000011 Rmu_sc0010674.1_g000003 Rmu_sc0011142.1_g000002 Rmu_sc0011514.1_g000004 Rmu_sc0011534.1_g000006 Rmu_sc0011803.1_g000007 Rmu_sc0013216.1_g000009 Rmu_sc0014482.1_g000002 Rmu_sc0015325.1_g000008 Rmu_sc0019673.1_g000001 Rmu_sc0021680.1_g000002 Rmu_sc0022642.1_g000001 Rmu_sc0023778.1_g000005 Rmu_sc0023942.1_g000001 Rmu_sc0024195.1_g000012 Rmu_sc0026069.1_g000002 Rmu_sc0026814.1_g000001 Rmu_sc0028519.1_g000001 Rmu_sc0031008.1_g000002 Rmu_sc0031197.1_g000001 Rmu_sc0031293.1_g000001 Rmu_sc0031294.1_g000001 Rmu_sc0031646.1_g000001 Rmu_sc0034742.1_g000002 Rmu_sc0036436.1_g000001 Rmu_sc0036437.1_g000001 Rmu_sc0042802.1_g000001 Rmu_ssc0000127.1_g000052 Rmu_ssc0000262.1_g000019 Rmu_ssc0000398.1_g000007 Rmu_ssc0000480.1_g000050
rosa_roxburghii Rroxscaffold_174G00435290 Rroxscaffold_177G00434320 Rroxscaffold_178G00437300 Rroxscaffold_180G00433410 Rroxscaffold_1G00010240 Rroxscaffold_1G00020730 Rroxscaffold_1G00059300 Rroxscaffold_2G00077380 Rroxscaffold_2G00077690 Rroxscaffold_3G00218240 Rroxscaffold_3G00223180 Rroxscaffold_3G00223190 Rroxscaffold_3G00255440 Rroxscaffold_3G00255450 Rroxscaffold_3G00259080 Rroxscaffold_3G00274700 Rroxscaffold_4G00306470 Rroxscaffold_4G00316750 Rroxscaffold_4G00323390 Rroxscaffold_4G00331230 Rroxscaffold_5G00339240 Rroxscaffold_5G00339550 Rroxscaffold_5G00378050 Rroxscaffold_6G00392800 Rroxscaffold_7G00163490 Rroxscaffold_7G00199370
rosa_wichuraiana Rw0G015150 Rw1G007650 Rw3G005560 Rw3G028190 Rw5G030760 Rw6G016740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 1023, 1507
Acc36I ACCTGC 1 cut(s) 497
AccB1I GGYRCC 1 cut(s) 163
AccB7I CCANNNNNTGG 1 cut(s) 193
AccI GTMKAC 2 cut(s) 732, 1473
AclWI GGATC 4 cut(s) 395, 796, 958, 971
AcoI YGGCCR 7 cut(s) 663, 677, 693, 952, 1061, 1522, 1788
AcsI RAATTY 2 cut(s) 214, 1803
AcyI GRCGYC 5 cut(s) 118, 164, 588, 1020, 1504
AfaI GTAC 6 cut(s) 769, 984, 1166, 1415, 1512, 1916
AfiI CCNNNNNNNGG 8 cut(s) 27, 193, 571, 598, 772, 1036, 1059, 1468
AgeI ACCGGT 1 cut(s) 1479
AgsI TTSAA 5 cut(s) 34, 102, 434, 1087, 1117
AjiI CACGTC 1 cut(s) 813
AjnI CCWGG 1 cut(s) 1849
AloI GAACNNNNNNTCC 2 cut(s) 1511, 1543
AluBI AGCT 1 cut(s) 1265
AluI AGCT 1 cut(s) 1265
Alw26I GTCTC 1 cut(s) 1535
AlwI GGATC 4 cut(s) 395, 796, 958, 971
AlwNI CAGNNNCTG 1 cut(s) 416
Ama87I CYCGRG 2 cut(s) 9, 131
AoxI GGCC 9 cut(s) 195, 562, 663, 677, 693, 952, 1061, 1522, 1788
ApaI GGGCCC 1 cut(s) 566
ApeKI GCWGC 7 cut(s) 345, 516, 549, 552, 612, 743, 1845
ApoI RAATTY 2 cut(s) 214, 1803
ArsI GACNNNNNNTTYG 2 cut(s) 1863, 1895
AsiGI ACCGGT 1 cut(s) 1479
Asp700I GAANNNNTTC 2 cut(s) 1254, 1392
AspA2I CCTAGG 1 cut(s) 856
AspLEI GCGC 7 cut(s) 166, 795, 929, 1665, 1865, 1923, 1925
AspS9I GGNCC 6 cut(s) 88, 196, 406, 562, 563, 1297
AsuC2I CCSGG 4 cut(s) 269, 838, 1452, 1463
AsuHPI GGTGA 2 cut(s) 286, 826
AsuII TTCGAA 1 cut(s) 1869
AvaI CYCGRG 2 cut(s) 9, 131
AvaII GGWCC 3 cut(s) 88, 406, 1297
AvrII CCTAGG 1 cut(s) 856
AxyI CCTNAGG 1 cut(s) 1293
BaeGI GKGCMC 1 cut(s) 566
BalI TGGCCA 1 cut(s) 665
BamHI GGATCC 1 cut(s) 963
BanI GGYRCC 1 cut(s) 163
BanII GRGCYC 1 cut(s) 566
BarI GAAGNNNNNNTAC 2 cut(s) 1697, 1729
BauI CACGAG 1 cut(s) 1760
BbsI GAAGAC 4 cut(s) 48, 228, 270, 1383
BbvCI CCTCAGC 1 cut(s) 1815
BbvI GCAGC 7 cut(s) 357, 528, 561, 564, 624, 730, 1832
BccI CCATC 5 cut(s) 99, 315, 436, 1514, 1806
BceAI ACGGC 3 cut(s) 708, 1529, 1803
BcgI CGANNNNNNTGC 2 cut(s) 722, 756
BciT130I CCWGG 1 cut(s) 1851
BciVI GTATCC 2 cut(s) 25, 1787
BclI TGATCA 1 cut(s) 714
BcnI CCSGG 4 cut(s) 269, 838, 1452, 1463
BcoDI GTCTC 1 cut(s) 1535
BfaI CTAG 3 cut(s) 257, 782, 857
BfmI CTRYAG 3 cut(s) 490, 1107, 1332
BfoI RGCGCY 1 cut(s) 167
BfuAI ACCTGC 1 cut(s) 497
BfuI GTATCC 2 cut(s) 25, 1787
BglI GCCNNNNNGGC 1 cut(s) 161
BglII AGATCT 1 cut(s) 312
BlnI CCTAGG 1 cut(s) 856
Bme1390I CCNGG 5 cut(s) 269, 838, 1452, 1463, 1851
Bme18I GGWCC 3 cut(s) 88, 406, 1297
BmeT110I CYCGRG 2 cut(s) 9, 131
BmgBI CACGTC 1 cut(s) 813
BmgT120I GGNCC 6 cut(s) 88, 196, 406, 562, 563, 1297
BmiI GGNNCC 6 cut(s) 6, 57, 165, 408, 564, 965
BmrFI CCNGG 5 cut(s) 269, 838, 1452, 1463, 1851
BmrI ACTGGG 3 cut(s) 404, 560, 1177
BmsI GCATC 6 cut(s) 624, 902, 1588, 1599, 1650, 1735
BmuI ACTGGG 3 cut(s) 404, 560, 1177
BoxI GACNNNNGTC 1 cut(s) 1503
BpiI GAAGAC 4 cut(s) 48, 228, 270, 1383
Bpu10I CCTNAGC 1 cut(s) 1815
Bpu14I TTCGAA 1 cut(s) 1869
BpuEI CTTGAG 1 cut(s) 123
BpuMI CCSGG 4 cut(s) 269, 838, 1452, 1463
Bsa29I ATCGAT 1 cut(s) 1582
BsaBI GATNNNNATC 1 cut(s) 311
BsaHI GRCGYC 5 cut(s) 118, 164, 588, 1020, 1504
BsaWI WCCGGW 1 cut(s) 1479
Bsc4I CCNNNNNNNGG 8 cut(s) 27, 193, 571, 598, 772, 1036, 1059, 1468
Bse118I RCCGGY 3 cut(s) 889, 1479, 1524
Bse1I ACTGG 6 cut(s) 171, 410, 566, 738, 1172, 1508
Bse21I CCTNAGG 1 cut(s) 1293
Bse8I GATNNNNATC 1 cut(s) 311
BseBI CCWGG 1 cut(s) 1851
BseCI ATCGAT 1 cut(s) 1582
BseGI GGATG 5 cut(s) 326, 447, 529, 786, 1525
BseJI GATNNNNATC 1 cut(s) 311
BseLI CCNNNNNNNGG 8 cut(s) 27, 193, 571, 598, 772, 1036, 1059, 1468
BseMII CTCAG 5 cut(s) 330, 1307, 1530, 1559, 1806
BseNI ACTGG 6 cut(s) 171, 410, 566, 738, 1172, 1508
BsePI GCGCGC 1 cut(s) 1921
BseRI GAGGAG 1 cut(s) 48
BseSI GKGCMC 1 cut(s) 566
BseX3I CGGCCG 2 cut(s) 693, 1788
BseXI GCAGC 7 cut(s) 357, 528, 561, 564, 624, 730, 1832
BseYI CCCAGC 1 cut(s) 198
Bsh1285I CGRYCG 4 cut(s) 696, 1501, 1586, 1791
BshFI GGCC 9 cut(s) 197, 564, 665, 679, 695, 954, 1063, 1524, 1790
BshNI GGYRCC 1 cut(s) 163
BshTI ACCGGT 1 cut(s) 1479
BshVI ATCGAT 1 cut(s) 1582
BsiEI CGRYCG 4 cut(s) 696, 1501, 1586, 1791
BsiHKCI CYCGRG 2 cut(s) 9, 131
BsiSI CCGG 9 cut(s) 268, 572, 837, 890, 1452, 1463, 1480, 1525, 1791
BslFI GGGAC 8 cut(s) 284, 392, 789, 1031, 1342, 1444, 1619, 1770
BslI CCNNNNNNNGG 8 cut(s) 27, 193, 571, 598, 772, 1036, 1059, 1468
BsmAI GTCTC 1 cut(s) 1535
BsmFI GGGAC 8 cut(s) 284, 392, 789, 1031, 1342, 1444, 1619, 1770
BsmI GAATGC 1 cut(s) 929
BsnI GGCC 9 cut(s) 197, 564, 665, 679, 695, 954, 1063, 1524, 1790
BsoBI CYCGRG 2 cut(s) 9, 131
Bsp119I TTCGAA 1 cut(s) 1869
Bsp120I GGGCCC 1 cut(s) 562
Bsp1286I GDGCHC 1 cut(s) 566
Bsp143I GATC 9 cut(s) 312, 387, 714, 801, 963, 1548, 1583, 1822, 1962
Bsp19I CCATGG 1 cut(s) 771
Bsp68I TCGCGA 1 cut(s) 1199
BspANI GGCC 9 cut(s) 197, 564, 665, 679, 695, 954, 1063, 1524, 1790
BspCNI CTCAG 5 cut(s) 329, 1306, 1531, 1558, 1807
BspDI ATCGAT 1 cut(s) 1582
BspHI TCATGA 2 cut(s) 1008, 1942
BspLI GGNNCC 6 cut(s) 6, 57, 165, 408, 564, 965
BspMAI CTGCAG 2 cut(s) 494, 1336
BspMI ACCTGC 1 cut(s) 497
BspPI GGATC 4 cut(s) 395, 796, 958, 971
BspT104I TTCGAA 1 cut(s) 1869
BspT107I GGYRCC 1 cut(s) 163
BsrFI RCCGGY 3 cut(s) 889, 1479, 1524
BsrI ACTGG 6 cut(s) 171, 410, 566, 738, 1172, 1508
BssAI RCCGGY 3 cut(s) 889, 1479, 1524
BssHII GCGCGC 1 cut(s) 1921
BssMI GATC 9 cut(s) 312, 387, 714, 801, 963, 1548, 1583, 1822, 1962
BssNI GRCGYC 5 cut(s) 118, 164, 588, 1020, 1504
BssSI CACGAG 1 cut(s) 1760
BssT1I CCWWGG 6 cut(s) 84, 402, 771, 856, 1242, 1275
Bst2BI CACGAG 1 cut(s) 1760
Bst2UI CCWGG 1 cut(s) 1851
Bst4CI ACNGT 5 cut(s) 30, 684, 823, 1031, 1682
BstACI GRCGYC 5 cut(s) 118, 164, 588, 1020, 1504
BstBI TTCGAA 1 cut(s) 1869
BstC8I GCNNGC 8 cut(s) 282, 863, 891, 1126, 1135, 1577, 1861, 1923
BstDEI CTNAG 7 cut(s) 316, 1261, 1293, 1365, 1539, 1545, 1815
BstDSI CCRYGG 4 cut(s) 122, 771, 1058, 1752
BstEII GGTNACC 1 cut(s) 1482
BstF5I GGATG 5 cut(s) 326, 447, 529, 786, 1525
BstH2I RGCGCY 1 cut(s) 167
BstHHI GCGC 7 cut(s) 166, 795, 929, 1665, 1865, 1923, 1925
BstKTI GATC 9 cut(s) 315, 390, 717, 804, 966, 1551, 1586, 1825, 1965
BstMAI GTCTC 1 cut(s) 1535
BstMBI GATC 9 cut(s) 312, 387, 714, 801, 963, 1548, 1583, 1822, 1962
BstMCI CGRYCG 4 cut(s) 696, 1501, 1586, 1791
BstNI CCWGG 1 cut(s) 1851
BstNSI RCATGY 2 cut(s) 676, 1579
BstPAI GACNNNNGTC 1 cut(s) 1503
BstPI GGTNACC 1 cut(s) 1482
BstSCI CCNGG 5 cut(s) 267, 836, 1450, 1461, 1849
BstSFI CTRYAG 3 cut(s) 490, 1107, 1332
BstSLI GKGCMC 1 cut(s) 566
BstV1I GCAGC 7 cut(s) 357, 528, 561, 564, 624, 730, 1832
BstV2I GAAGAC 4 cut(s) 48, 228, 270, 1383
BstX2I RGATCY 2 cut(s) 312, 963
BstXI CCANNNNNNTGG 1 cut(s) 1726
BstYI RGATCY 2 cut(s) 312, 963
BstZI CGGCCG 2 cut(s) 693, 1788
Bsu15I ATCGAT 1 cut(s) 1582
Bsu36I CCTNAGG 1 cut(s) 1293
BsuI GTATCC 2 cut(s) 25, 1787
BsuRI GGCC 9 cut(s) 197, 564, 665, 679, 695, 954, 1063, 1524, 1790
BsuTUI ATCGAT 1 cut(s) 1582
BtgI CCRYGG 4 cut(s) 122, 771, 1058, 1752
BtrI CACGTC 1 cut(s) 813
BtsCI GGATG 5 cut(s) 326, 447, 529, 786, 1525
BtsI GCAGTG 1 cut(s) 499
BtsIMutI CAGTG 4 cut(s) 499, 680, 745, 1366
BtuMI TCGCGA 1 cut(s) 1199
BveI ACCTGC 1 cut(s) 497
Cac8I GCNNGC 8 cut(s) 282, 863, 891, 1126, 1135, 1577, 1861, 1923
CaiI CAGNNNCTG 1 cut(s) 416
CciI TCATGA 2 cut(s) 1008, 1942
CfoI GCGC 7 cut(s) 166, 795, 929, 1665, 1865, 1923, 1925
Cfr10I RCCGGY 3 cut(s) 889, 1479, 1524
Cfr13I GGNCC 6 cut(s) 88, 196, 406, 562, 563, 1297
Cfr42I CCGCGG 3 cut(s) 125, 1061, 1755
ClaI ATCGAT 1 cut(s) 1582
CseI GACGC 4 cut(s) 107, 365, 596, 1654
Csp6I GTAC 6 cut(s) 768, 983, 1165, 1414, 1511, 1915
CspAI ACCGGT 1 cut(s) 1479
CviQI GTAC 6 cut(s) 768, 983, 1165, 1414, 1511, 1915
DdeI CTNAG 7 cut(s) 316, 1261, 1293, 1365, 1539, 1545, 1815
DinI GGCGCC 1 cut(s) 165
DpnI GATC 9 cut(s) 314, 389, 716, 803, 965, 1550, 1585, 1824, 1964
DpnII GATC 9 cut(s) 312, 387, 714, 801, 963, 1548, 1583, 1822, 1962
EaeI YGGCCR 7 cut(s) 663, 677, 693, 952, 1061, 1522, 1788
EagI CGGCCG 2 cut(s) 693, 1788
EciI GGCGGA 4 cut(s) 7, 515, 1066, 1388
EclXI CGGCCG 2 cut(s) 693, 1788
Eco130I CCWWGG 6 cut(s) 84, 402, 771, 856, 1242, 1275
Eco24I GRGCYC 1 cut(s) 566
Eco47I GGWCC 3 cut(s) 88, 406, 1297
Eco52I CGGCCG 2 cut(s) 693, 1788
Eco81I CCTNAGG 1 cut(s) 1293
Eco88I CYCGRG 2 cut(s) 9, 131
Eco91I GGTNACC 1 cut(s) 1482
EcoO65I GGTNACC 1 cut(s) 1482
EcoRI GAATTC 1 cut(s) 214
EcoRII CCWGG 1 cut(s) 1849
EcoT14I CCWWGG 6 cut(s) 84, 402, 771, 856, 1242, 1275
EcoT22I ATGCAT 1 cut(s) 1581
EcoT38I GRGCYC 1 cut(s) 566
EgeI GGCGCC 1 cut(s) 165
EheI GGCGCC 1 cut(s) 165
ErhI CCWWGG 6 cut(s) 84, 402, 771, 856, 1242, 1275
FalI AAGNNNNNCTT 2 cut(s) 1380, 1412
FaqI GGGAC 8 cut(s) 284, 392, 789, 1031, 1342, 1444, 1619, 1770
FauI CCCGC 5 cut(s) 549, 798, 1065, 1276, 1747
FbaI TGATCA 1 cut(s) 714
FblI GTMKAC 2 cut(s) 732, 1473
FokI GGATG 5 cut(s) 333, 454, 536, 773, 1532
FriOI GRGCYC 1 cut(s) 566
FspBI CTAG 3 cut(s) 257, 782, 857
GlaI GCGC 7 cut(s) 165, 794, 928, 1664, 1864, 1922, 1924
GsaI CCCAGC 1 cut(s) 202
HaeII RGCGCY 1 cut(s) 167
HaeIII GGCC 9 cut(s) 197, 564, 665, 679, 695, 954, 1063, 1524, 1790
HapII CCGG 9 cut(s) 268, 572, 837, 890, 1452, 1463, 1480, 1525, 1791
HgaI GACGC 4 cut(s) 107, 365, 596, 1654
HhaI GCGC 7 cut(s) 166, 795, 929, 1665, 1865, 1923, 1925
Hin1I GRCGYC 5 cut(s) 118, 164, 588, 1020, 1504
Hin6I GCGC 7 cut(s) 164, 793, 927, 1663, 1863, 1921, 1923
HinP1I GCGC 7 cut(s) 164, 793, 927, 1663, 1863, 1921, 1923
HincII GTYRAC 2 cut(s) 733, 1321
HindII GTYRAC 2 cut(s) 733, 1321
HinfI GANTC 5 cut(s) 777, 946, 1139, 1155, 1543
HpaII CCGG 9 cut(s) 268, 572, 837, 890, 1452, 1463, 1480, 1525, 1791
HphI GGTGA 2 cut(s) 286, 826
Hpy166II GTNNAC 4 cut(s) 733, 904, 1321, 1474
Hpy188I TCNGA 5 cut(s) 220, 241, 460, 1548, 1892
Hpy8I GTNNAC 4 cut(s) 733, 904, 1321, 1474
Hpy99I CGWCG 7 cut(s) 230, 587, 590, 920, 1025, 1506, 1814
HpyCH4III ACNGT 5 cut(s) 30, 684, 823, 1031, 1682
HpyCH4IV ACGT 5 cut(s) 812, 1020, 1323, 1504, 1913
HpyCH4V TGCA 5 cut(s) 492, 549, 997, 1334, 1579
HpyF3I CTNAG 7 cut(s) 316, 1261, 1293, 1365, 1539, 1545, 1815
HpySE526I ACGT 5 cut(s) 812, 1020, 1323, 1504, 1913
Hsp92I GRCGYC 5 cut(s) 118, 164, 588, 1020, 1504
HspAI GCGC 7 cut(s) 164, 793, 927, 1663, 1863, 1921, 1923
KasI GGCGCC 1 cut(s) 163
KroI GCCGGC 1 cut(s) 889
KroNI GCCGGC 1 cut(s) 891
Ksp22I TGATCA 1 cut(s) 714
KspI CCGCGG 3 cut(s) 125, 1061, 1755
Kzo9I GATC 9 cut(s) 312, 387, 714, 801, 963, 1548, 1583, 1822, 1962
LmnI GCTCC 2 cut(s) 61, 1270
Lsp1109I GCAGC 7 cut(s) 357, 528, 561, 564, 624, 730, 1832
LweI GCATC 6 cut(s) 624, 902, 1588, 1599, 1650, 1735
MaeI CTAG 3 cut(s) 257, 782, 857
MaeII ACGT 5 cut(s) 812, 1020, 1323, 1504, 1913
MaeIII GTNAC 3 cut(s) 757, 1482, 1682
MalI GATC 9 cut(s) 314, 389, 716, 803, 965, 1550, 1585, 1824, 1964
MboI GATC 9 cut(s) 312, 387, 714, 801, 963, 1548, 1583, 1822, 1962
MboII GAAGA 7 cut(s) 53, 233, 275, 442, 1388, 1454, 1832
MflI RGATCY 2 cut(s) 312, 963
MhlI GDGCHC 1 cut(s) 566
MlsI TGGCCA 1 cut(s) 665
MluCI AATT 4 cut(s) 214, 422, 603, 1803
MluNI TGGCCA 1 cut(s) 665
Mly113I GGCGCC 1 cut(s) 164
MlyI GAGTC 4 cut(s) 771, 1148, 1149, 1537
Mox20I TGGCCA 1 cut(s) 665
Mph1103I ATGCAT 1 cut(s) 1581
MroNI GCCGGC 1 cut(s) 889
MroXI GAANNNNTTC 2 cut(s) 1254, 1392
MscI TGGCCA 1 cut(s) 665
MseI TTAA 3 cut(s) 252, 818, 1896
MslI CAYNNNNRTG 3 cut(s) 671, 1598, 1724
Msp20I TGGCCA 1 cut(s) 665
MspA1I CMGCKG 4 cut(s) 124, 1060, 1754, 1845
MspI CCGG 9 cut(s) 268, 572, 837, 890, 1452, 1463, 1480, 1525, 1791
MspR9I CCNGG 5 cut(s) 269, 838, 1452, 1463, 1851
Mva1269I GAATGC 1 cut(s) 929
MvaI CCWGG 1 cut(s) 1851
NaeI GCCGGC 1 cut(s) 891
NarI GGCGCC 1 cut(s) 164
NciI CCSGG 4 cut(s) 269, 838, 1452, 1463
NcoI CCATGG 1 cut(s) 771
NdeII GATC 9 cut(s) 312, 387, 714, 801, 963, 1548, 1583, 1822, 1962
NgoMIV GCCGGC 1 cut(s) 889
NlaIV GGNNCC 6 cut(s) 6, 57, 165, 408, 564, 965
NmeAIII GCCGAG 1 cut(s) 980
NmuCI GTSAC 2 cut(s) 757, 1682
NruI TCGCGA 1 cut(s) 1199
NsiI ATGCAT 1 cut(s) 1581
NspI RCATGY 2 cut(s) 676, 1579
NspV TTCGAA 1 cut(s) 1869
PaeI GCATGC 1 cut(s) 1579
PagI TCATGA 2 cut(s) 1008, 1942
PauI GCGCGC 1 cut(s) 1921
PcsI WCGNNNNNNNCGW 1 cut(s) 369
PctI GAATGC 1 cut(s) 929
PdiI GCCGGC 1 cut(s) 891
PdmI GAANNNNTTC 2 cut(s) 1254, 1392
PfeI GAWTC 1 cut(s) 946
PflFI GACNNNGTC 2 cut(s) 1193, 1469
PflMI CCANNNNNTGG 1 cut(s) 193
PinAI ACCGGT 1 cut(s) 1479
Ple19I CGATCG 1 cut(s) 1586
PleI GAGTC 4 cut(s) 771, 1147, 1149, 1537
PluTI GGCGCC 1 cut(s) 167
PpsI GAGTC 4 cut(s) 771, 1147, 1149, 1537
PshAI GACNNNNGTC 1 cut(s) 1503
Psp6I CCWGG 1 cut(s) 1849
PspEI GGTNACC 1 cut(s) 1482
PspFI CCCAGC 1 cut(s) 198
PspGI CCWGG 1 cut(s) 1849
PspN4I GGNNCC 6 cut(s) 6, 57, 165, 408, 564, 965
PspOMI GGGCCC 1 cut(s) 562
PspPI GGNCC 6 cut(s) 88, 196, 406, 562, 563, 1297
PstI CTGCAG 2 cut(s) 494, 1336
PstNI CAGNNNCTG 1 cut(s) 416
PsuI RGATCY 2 cut(s) 312, 963
PsyI GACNNNGTC 2 cut(s) 1193, 1469
PteI GCGCGC 1 cut(s) 1921
PvuI CGATCG 1 cut(s) 1586
RruI TCGCGA 1 cut(s) 1199
RsaI GTAC 6 cut(s) 769, 984, 1166, 1415, 1512, 1916
RsaNI GTAC 6 cut(s) 768, 983, 1165, 1414, 1511, 1915
RseI CAYNNNNRTG 3 cut(s) 671, 1598, 1724
SacII CCGCGG 3 cut(s) 125, 1061, 1755
SalI GTCGAC 1 cut(s) 731
SaqAI TTAA 3 cut(s) 252, 818, 1896
Sau3AI GATC 9 cut(s) 312, 387, 714, 801, 963, 1548, 1583, 1822, 1962
Sau96I GGNCC 6 cut(s) 88, 196, 406, 562, 563, 1297
SchI GAGTC 4 cut(s) 771, 1148, 1149, 1537
ScrFI CCNGG 5 cut(s) 269, 838, 1452, 1463, 1851
SduI GDGCHC 1 cut(s) 566
SfaNI GCATC 6 cut(s) 624, 902, 1588, 1599, 1650, 1735
SfcI CTRYAG 3 cut(s) 490, 1107, 1332
SfoI GGCGCC 1 cut(s) 165
Sfr303I CCGCGG 3 cut(s) 125, 1061, 1755
SfuI TTCGAA 1 cut(s) 1869
SgrBI CCGCGG 3 cut(s) 125, 1061, 1755
SinI GGWCC 3 cut(s) 88, 406, 1297
SmiMI CAYNNNNRTG 3 cut(s) 671, 1598, 1724
SmlI CTYRAG 1 cut(s) 138
SmoI CTYRAG 1 cut(s) 138
SphI GCATGC 1 cut(s) 1579
Sse9I AATT 4 cut(s) 214, 422, 603, 1803
SspDI GGCGCC 1 cut(s) 163
SspMI CTAG 3 cut(s) 257, 782, 857
StyD4I CCNGG 5 cut(s) 267, 836, 1450, 1461, 1849
StyI CCWWGG 6 cut(s) 84, 402, 771, 856, 1242, 1275
TaaI ACNGT 5 cut(s) 30, 684, 823, 1031, 1682
TaiI ACGT 5 cut(s) 815, 1023, 1326, 1507, 1916
TaqII GACCGA 1 cut(s) 1515
TasI AATT 4 cut(s) 214, 422, 603, 1803
TatI WGTACW 1 cut(s) 1413
TauI GCSGC 8 cut(s) 127, 158, 500, 679, 1063, 1274, 1751, 1754
TfiI GAWTC 1 cut(s) 946
Tru1I TTAA 3 cut(s) 252, 818, 1896
Tru9I TTAA 3 cut(s) 252, 818, 1896
TscAI CASTG 4 cut(s) 499, 687, 745, 1366
TseFI GTSAC 2 cut(s) 757, 1682
TseI GCWGC 7 cut(s) 345, 516, 549, 552, 612, 743, 1845
Tsp45I GTSAC 2 cut(s) 757, 1682
TspDTI ATGAA 5 cut(s) 348, 442, 1519, 1931, 1943
TspGWI ACGGA 1 cut(s) 1797
TspRI CASTG 4 cut(s) 499, 687, 745, 1366
Tth111I GACNNNGTC 2 cut(s) 1193, 1469
Van91I CCANNNNNTGG 1 cut(s) 193
VpaK11BI GGWCC 3 cut(s) 88, 406, 1297
XapI RAATTY 2 cut(s) 214, 1803
XceI RCATGY 2 cut(s) 676, 1579
XcmI CCANNNNNNNNNTGG 1 cut(s) 652
XmaJI CCTAGG 1 cut(s) 856
XmiI GTMKAC 2 cut(s) 732, 1473
XmnI GAANNNNTTC 2 cut(s) 1254, 1392
XspI CTAG 3 cut(s) 257, 782, 857
ZraI GACGTC 2 cut(s) 1021, 1505
Zsp2I ATGCAT 1 cut(s) 1581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.