Rroxscaffold_177G00434320

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000177
OG0000176
Physical Location & Seq
Forward (+)
256267 .. 257520
1254 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_177G00434320.1

Sequence Viewer

Length: 873 bp
ATGCAACTGCTCGGAATCAAGAAAGAGAAAATCCAGTCTACATCCCTCACGCTCAAGAACTTCACGGGAACCGTGACGAAGACCTTGGGACCGCTGTTCTTACGCATTAGGTTAGCCCCGCAGAGGAGTTTATGCTTTCTTTGTGACAGATCGTTATGCGGCCTACATTGCTATTCGGGCGGAGACCGGATCCACCGGAGTTACCGTGTTCCGTCAACTCTCTACCGAGAACCGATTATATGGAACAGGGAGACAGATAAAGCCGAAGTGATCAAGGCGGACCCTCGTCCATTCCCGTTGTCCGCGAATTATGTAGATGCCGAGTACTATTTGGAGCCTATCACTCCATTGCGGTCGGTGGGCATTGATGATAAAGGCCGCCCCACGGGGGTGACCGCCTCGAATTGGAGCAAGAGGGGCTTACACTCGCAAAACAGATGCGCCTATGGAAGTGGCTATCGCGCTTACTTGGAGCCTAGCGAGAAACAGAGGCTCATTGACTTATTACTAGAATTCAAAGATTGCTTCGCGGAAAAGTATGAAGACATGCCCGCCCGTCACCGGACTTGGTTTGCCATCAACGCAACGCTCCCCGACAAGAGGCCTCGTGAAACAAGAGCCGCGGAGAATGAATTCTGCCTCGTTCGCAAGAAGAATGGCAAGATGAGGGTTTGTGTAGACTACGGGGACCTTAATATCGCCACGCCTAAGGATGTCTACCCCATGCCGGTCGCGGATATGTTAGCGTACGCCCGTGGCAGGCATGAATTATTGTCCTTCATGGACGGCACCGCAGATATCACCGATTCCGGTTGCAGAGGAAGACAGAGACATAAACCGCATTCCGGTGTCCCGGTTCGTGGGCGTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

290

Amino Acids

33.11

Weight (kDa)

9.48

Isoelectric Point (pI)

33.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000176)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21383 FvH4_2g06271 FvH4_2g23662 FvH4_2g35580 FvH4_3g27863 FvH4_4g05274 FvH4_4g18553 FvH4_4g21611 FvH4_5g26253 FvH4_5g34301 FvH4_6g31922 FvH4_7g12391
pyrus_communis pycom03g12260 pycom10g07780 pycom12g20020 pycom15g29760 pycom16g11740 pycom976g00240
rosa_chinensis RchiOBHm_Chr2g0121061 RchiOBHm_Chr5g0033131 RchiOBHm_Chr6g0263211
rosa_multiflora Rmu_co7975030.1_g000001 Rmu_co8030416.1_g000001 Rmu_co8076880.1_g000001 Rmu_co8153002.1_g000001 Rmu_co8164780.1_g000001 Rmu_co8199530.1_g000001 Rmu_co8226177.1_g000002 Rmu_co8305941.1_g000001 Rmu_co8331357.1_g000001 Rmu_co8346103.1_g000001 Rmu_co8386779.1_g000001 Rmu_co8415559.1_g000002 Rmu_co8420415.1_g000002 Rmu_co8517321.1_g000001 Rmu_co8522293.1_g000007 Rmu_sc0000029.1_g000020 Rmu_sc0000147.1_g000010 Rmu_sc0000240.1_g000068 Rmu_sc0000276.1_g000040 Rmu_sc0000322.1_g000020 Rmu_sc0000338.1_g000010 Rmu_sc0000347.1_g000012 Rmu_sc0000461.1_g000002 Rmu_sc0000559.1_g000028 Rmu_sc0000594.1_g000033 Rmu_sc0000613.1_g000009 Rmu_sc0000704.1_g000027 Rmu_sc0000740.1_g000054 Rmu_sc0001051.1_g000021 Rmu_sc0001069.1_g000038 Rmu_sc0001113.1_g000021 Rmu_sc0001250.1_g000030 Rmu_sc0001395.1_g000017 Rmu_sc0001396.1_g000005 Rmu_sc0001738.1_g000043 Rmu_sc0001887.1_g000006 Rmu_sc0001898.1_g000006 Rmu_sc0002014.1_g000019 Rmu_sc0002270.1_g000036 Rmu_sc0002532.1_g000023 Rmu_sc0002638.1_g000047 Rmu_sc0002690.1_g000020 Rmu_sc0002784.1_g000048 Rmu_sc0002882.1_g000024 Rmu_sc0002934.1_g000006 Rmu_sc0002934.1_g000007 Rmu_sc0002938.1_g000028 Rmu_sc0003044.1_g000017 Rmu_sc0003252.1_g000007 Rmu_sc0003737.1_g000023 Rmu_sc0003809.1_g000028 Rmu_sc0003809.1_g000029 Rmu_sc0003936.1_g000019 Rmu_sc0004001.1_g000011 Rmu_sc0004059.1_g000014 Rmu_sc0004101.1_g000022 Rmu_sc0004174.1_g000017 Rmu_sc0004206.1_g000017 Rmu_sc0004206.1_g000018 Rmu_sc0004243.1_g000008 Rmu_sc0004283.1_g000024 Rmu_sc0004549.1_g000015 Rmu_sc0004718.1_g000011 Rmu_sc0004941.1_g000039 Rmu_sc0005112.1_g000017 Rmu_sc0005125.1_g000028 Rmu_sc0005143.1_g000007 Rmu_sc0005147.1_g000009 Rmu_sc0005260.1_g000001 Rmu_sc0005322.1_g000010 Rmu_sc0005454.1_g000008 Rmu_sc0005652.1_g000020 Rmu_sc0005659.1_g000006 Rmu_sc0006059.1_g000057 Rmu_sc0006129.1_g000021 Rmu_sc0006300.1_g000007 Rmu_sc0006561.1_g000011 Rmu_sc0007612.1_g000036 Rmu_sc0007856.1_g000004 Rmu_sc0009191.1_g000019 Rmu_sc0009191.1_g000020 Rmu_sc0009481.1_g000010 Rmu_sc0009901.1_g000002 Rmu_sc0009968.1_g000001 Rmu_sc0010072.1_g000006 Rmu_sc0010090.1_g000011 Rmu_sc0010674.1_g000003 Rmu_sc0011142.1_g000002 Rmu_sc0011514.1_g000004 Rmu_sc0011534.1_g000006 Rmu_sc0011803.1_g000007 Rmu_sc0013216.1_g000009 Rmu_sc0014482.1_g000002 Rmu_sc0015325.1_g000008 Rmu_sc0019673.1_g000001 Rmu_sc0021680.1_g000002 Rmu_sc0022642.1_g000001 Rmu_sc0023778.1_g000005 Rmu_sc0023942.1_g000001 Rmu_sc0024195.1_g000012 Rmu_sc0026069.1_g000002 Rmu_sc0026814.1_g000001 Rmu_sc0028519.1_g000001 Rmu_sc0031008.1_g000002 Rmu_sc0031197.1_g000001 Rmu_sc0031293.1_g000001 Rmu_sc0031294.1_g000001 Rmu_sc0031646.1_g000001 Rmu_sc0034742.1_g000002 Rmu_sc0036436.1_g000001 Rmu_sc0036437.1_g000001 Rmu_sc0042802.1_g000001 Rmu_ssc0000127.1_g000052 Rmu_ssc0000262.1_g000019 Rmu_ssc0000398.1_g000007 Rmu_ssc0000480.1_g000050
rosa_roxburghii Rroxscaffold_174G00435290 Rroxscaffold_177G00434320 Rroxscaffold_178G00437300 Rroxscaffold_180G00433410 Rroxscaffold_1G00010240 Rroxscaffold_1G00020730 Rroxscaffold_1G00059300 Rroxscaffold_2G00077380 Rroxscaffold_2G00077690 Rroxscaffold_3G00218240 Rroxscaffold_3G00223180 Rroxscaffold_3G00223190 Rroxscaffold_3G00255440 Rroxscaffold_3G00255450 Rroxscaffold_3G00259080 Rroxscaffold_3G00274700 Rroxscaffold_4G00306470 Rroxscaffold_4G00316750 Rroxscaffold_4G00323390 Rroxscaffold_4G00331230 Rroxscaffold_5G00339240 Rroxscaffold_5G00339550 Rroxscaffold_5G00378050 Rroxscaffold_6G00392800 Rroxscaffold_7G00163490 Rroxscaffold_7G00199370
rosa_wichuraiana Rw0G015150 Rw1G007650 Rw3G005560 Rw3G028190 Rw5G030760 Rw6G016740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 788
AccI GTMKAC 3 cut(s) 38, 678, 717
AccII CGCG 5 cut(s) 305, 462, 530, 623, 734
AclWI GGATC 2 cut(s) 184, 197
AcsI RAATTY 2 cut(s) 512, 632
AfaI GTAC 2 cut(s) 326, 749
AgsI TTSAA 1 cut(s) 517
AleI CACNNNNGTG 1 cut(s) 389
Alw26I GTCTC 3 cut(s) 177, 245, 823
AlwI GGATC 2 cut(s) 184, 197
AoxI GGCC 3 cut(s) 160, 376, 602
ApoI RAATTY 2 cut(s) 512, 632
Asp700I GAANNNNTTC 1 cut(s) 632
AspLEI GCGC 2 cut(s) 443, 464
AspS9I GGNCC 3 cut(s) 89, 280, 688
AsuC2I CCSGG 1 cut(s) 854
AsuHPI GGTGA 3 cut(s) 403, 551, 793
AvaII GGWCC 3 cut(s) 89, 280, 688
AxyI CCTNAGG 1 cut(s) 708
BamHI GGATCC 1 cut(s) 189
BanI GGYRCC 1 cut(s) 788
BauI CACGAG 1 cut(s) 606
BbsI GAAGAC 3 cut(s) 86, 549, 829
BccI CCATC 1 cut(s) 584
BceAI ACGGC 1 cut(s) 802
BclI TGATCA 1 cut(s) 270
BcnI CCSGG 1 cut(s) 854
BcoDI GTCTC 3 cut(s) 177, 245, 823
BfaI CTAG 2 cut(s) 477, 509
BisI GCNGC 3 cut(s) 160, 379, 621
BlsI GCNGC 3 cut(s) 161, 380, 622
BmcAI AGTACT 1 cut(s) 326
Bme1390I CCNGG 1 cut(s) 854
Bme18I GGWCC 3 cut(s) 89, 280, 688
BmgT120I GGNCC 3 cut(s) 89, 280, 688
BmiI GGNNCC 8 cut(s) 70, 90, 191, 282, 336, 474, 689, 790
BmrFI CCNGG 1 cut(s) 854
BmsI GCATC 2 cut(s) 307, 428
BoxI GACNNNNGTC 1 cut(s) 285
BpiI GAAGAC 3 cut(s) 86, 549, 829
BpuEI CTTGAG 1 cut(s) 38
BpuMI CCSGG 1 cut(s) 854
BsaI GGTCTC 1 cut(s) 177
BsaJI CCNNGG 4 cut(s) 84, 384, 621, 754
BsaWI WCCGGW 5 cut(s) 186, 195, 561, 809, 845
BsaXI ACNNNNNCTCC 2 cut(s) 190, 220
Bse118I RCCGGY 1 cut(s) 727
Bse1I ACTGG 1 cut(s) 34
Bse21I CCTNAGG 1 cut(s) 708
Bse3DI GCAATG 2 cut(s) 166, 347
BseDI CCNNGG 4 cut(s) 84, 384, 621, 754
BseGI GGATG 2 cut(s) 41, 718
BseMI GCAATG 2 cut(s) 166, 347
BseNI ACTGG 1 cut(s) 34
BseRI GAGGAG 1 cut(s) 139
Bsh1236I CGCG 5 cut(s) 305, 462, 530, 623, 734
Bsh1285I CGRYCG 2 cut(s) 356, 732
BshFI GGCC 3 cut(s) 162, 378, 604
BshNI GGYRCC 1 cut(s) 788
BsiEI CGRYCG 2 cut(s) 356, 732
BsiSI CCGG 7 cut(s) 187, 196, 562, 728, 810, 846, 854
BsiWI CGTACG 1 cut(s) 747
BslFI GGGAC 3 cut(s) 102, 701, 836
BsmAI GTCTC 3 cut(s) 177, 245, 823
BsmFI GGGAC 3 cut(s) 102, 701, 836
BsmI GAATGC 1 cut(s) 841
BsnI GGCC 3 cut(s) 162, 378, 604
Bso31I GGTCTC 1 cut(s) 177
Bsp143I GATC 3 cut(s) 149, 189, 270
BspANI GGCC 3 cut(s) 162, 378, 604
BspFNI CGCG 5 cut(s) 305, 462, 530, 623, 734
BspLI GGNNCC 8 cut(s) 70, 90, 191, 282, 336, 474, 689, 790
BspPI GGATC 2 cut(s) 184, 197
BspT107I GGYRCC 1 cut(s) 788
BspTNI GGTCTC 1 cut(s) 177
BsrDI GCAATG 2 cut(s) 166, 347
BsrFI RCCGGY 1 cut(s) 727
BsrI ACTGG 1 cut(s) 34
BssAI RCCGGY 1 cut(s) 727
BssECI CCNNGG 4 cut(s) 84, 384, 621, 754
BssMI GATC 3 cut(s) 149, 189, 270
BssSI CACGAG 1 cut(s) 606
BssT1I CCWWGG 1 cut(s) 84
Bst2BI CACGAG 1 cut(s) 606
Bst4CI ACNGT 2 cut(s) 73, 206
BstC8I GCNNGC 2 cut(s) 552, 761
BstDEI CTNAG 1 cut(s) 708
BstDSI CCRYGG 3 cut(s) 384, 621, 754
BstEII GGTNACC 1 cut(s) 391
BstF5I GGATG 2 cut(s) 41, 718
BstFNI CGCG 5 cut(s) 305, 462, 530, 623, 734
BstHHI GCGC 2 cut(s) 443, 464
BstKTI GATC 3 cut(s) 152, 192, 273
BstMAI GTCTC 3 cut(s) 177, 245, 823
BstMBI GATC 3 cut(s) 149, 189, 270
BstMCI CGRYCG 2 cut(s) 356, 732
BstMWI GCNNNNNNNGC 5 cut(s) 168, 177, 417, 581, 645
BstNSI RCATGY 1 cut(s) 550
BstPAI GACNNNNGTC 1 cut(s) 285
BstPI GGTNACC 1 cut(s) 391
BstSCI CCNGG 1 cut(s) 852
BstUI CGCG 5 cut(s) 305, 462, 530, 623, 734
BstV2I GAAGAC 3 cut(s) 86, 549, 829
BstX2I RGATCY 1 cut(s) 189
BstYI RGATCY 1 cut(s) 189
Bsu36I CCTNAGG 1 cut(s) 708
BsuRI GGCC 3 cut(s) 162, 378, 604
BtgI CCRYGG 3 cut(s) 384, 621, 754
BtsCI GGATG 2 cut(s) 41, 718
Cac8I GCNNGC 2 cut(s) 552, 761
CfoI GCGC 2 cut(s) 443, 464
Cfr10I RCCGGY 1 cut(s) 727
Cfr13I GGNCC 3 cut(s) 89, 280, 688
Cfr42I CCGCGG 1 cut(s) 624
Csp6I GTAC 2 cut(s) 325, 748
CviAII CATG 4 cut(s) 547, 724, 764, 781
CviQI GTAC 2 cut(s) 325, 748
DdeI CTNAG 1 cut(s) 708
DpnI GATC 3 cut(s) 151, 191, 272
DpnII GATC 3 cut(s) 149, 189, 270
EciI GGCGGA 2 cut(s) 195, 293
Eco130I CCWWGG 1 cut(s) 84
Eco147I AGGCCT 1 cut(s) 604
Eco31I GGTCTC 1 cut(s) 177
Eco32I GATATC 1 cut(s) 799
Eco47I GGWCC 3 cut(s) 89, 280, 688
Eco81I CCTNAGG 1 cut(s) 708
Eco91I GGTNACC 1 cut(s) 391
EcoO109I RGGNCCY 1 cut(s) 688
EcoO65I GGTNACC 1 cut(s) 391
EcoRI GAATTC 2 cut(s) 512, 632
EcoRV GATATC 1 cut(s) 799
EcoT14I CCWWGG 1 cut(s) 84
ErhI CCWWGG 1 cut(s) 84
FaeI CATG 4 cut(s) 550, 727, 767, 784
FalI AAGNNNNNCTT 2 cut(s) 404, 436
FaqI GGGAC 3 cut(s) 102, 701, 836
FatI CATG 4 cut(s) 546, 723, 763, 780
FauI CCCGC 2 cut(s) 126, 559
FbaI TGATCA 1 cut(s) 270
FblI GTMKAC 3 cut(s) 38, 678, 717
Fnu4HI GCNGC 3 cut(s) 160, 379, 621
FokI GGATG 2 cut(s) 28, 725
Fsp4HI GCNGC 3 cut(s) 160, 379, 621
FspBI CTAG 2 cut(s) 477, 509
GlaI GCGC 2 cut(s) 442, 463
GluI GCNGC 3 cut(s) 160, 379, 621
HaeIII GGCC 3 cut(s) 162, 378, 604
HapII CCGG 7 cut(s) 187, 196, 562, 728, 810, 846, 854
HhaI GCGC 2 cut(s) 443, 464
Hin1II CATG 4 cut(s) 550, 727, 767, 784
Hin6I GCGC 2 cut(s) 441, 462
HinP1I GCGC 2 cut(s) 441, 462
HincII GTYRAC 1 cut(s) 216
HindII GTYRAC 1 cut(s) 216
HinfI GANTC 2 cut(s) 15, 806
HpaII CCGG 7 cut(s) 187, 196, 562, 728, 810, 846, 854
HphI GGTGA 3 cut(s) 403, 551, 793
Hpy166II GTNNAC 4 cut(s) 39, 216, 679, 718
Hpy188I TCNGA 1 cut(s) 14
Hpy188III TCNNGA 3 cut(s) 19, 55, 608
Hpy8I GTNNAC 4 cut(s) 39, 216, 679, 718
HpyAV CCTTC 1 cut(s) 787
HpyCH4III ACNGT 2 cut(s) 73, 206
HpyCH4V TGCA 2 cut(s) 4, 816
HpyF10VI GCNNNNNNNGC 5 cut(s) 168, 177, 417, 581, 645
HpyF3I CTNAG 1 cut(s) 708
Hsp92II CATG 4 cut(s) 550, 727, 767, 784
HspAI GCGC 2 cut(s) 441, 462
Ksp22I TGATCA 1 cut(s) 270
KspI CCGCGG 1 cut(s) 624
Kzo9I GATC 3 cut(s) 149, 189, 270
LmnI GCTCC 4 cut(s) 334, 408, 472, 594
LweI GCATC 2 cut(s) 307, 428
MaeI CTAG 2 cut(s) 477, 509
MaeIII GTNAC 5 cut(s) 73, 143, 200, 391, 557
MalI GATC 3 cut(s) 151, 191, 272
MboI GATC 3 cut(s) 149, 189, 270
MboII GAAGA 4 cut(s) 91, 554, 664, 834
MflI RGATCY 1 cut(s) 189
MluCI AATT 5 cut(s) 307, 403, 512, 632, 767
MroXI GAANNNNTTC 1 cut(s) 632
MseI TTAA 2 cut(s) 693, 871
MslI CAYNNNNRTG 2 cut(s) 389, 846
MspA1I CMGCKG 2 cut(s) 94, 623
MspI CCGG 7 cut(s) 187, 196, 562, 728, 810, 846, 854
MspR9I CCNGG 1 cut(s) 854
Mva1269I GAATGC 1 cut(s) 841
MvnI CGCG 5 cut(s) 305, 462, 530, 623, 734
MwoI GCNNNNNNNGC 5 cut(s) 168, 177, 417, 581, 645
NciI CCSGG 1 cut(s) 854
NdeII GATC 3 cut(s) 149, 189, 270
NlaIII CATG 4 cut(s) 550, 727, 767, 784
NlaIV GGNNCC 8 cut(s) 70, 90, 191, 282, 336, 474, 689, 790
NmeAIII GCCGAG 1 cut(s) 346
NmuCI GTSAC 4 cut(s) 73, 143, 391, 557
NspI RCATGY 1 cut(s) 550
OliI CACNNNNGTG 1 cut(s) 389
PceI AGGCCT 1 cut(s) 604
PctI GAATGC 1 cut(s) 841
PdmI GAANNNNTTC 1 cut(s) 632
PfeI GAWTC 2 cut(s) 15, 806
Pfl23II CGTACG 1 cut(s) 747
PkrI GCNGC 3 cut(s) 161, 380, 622
PpuMI RGGWCCY 1 cut(s) 688
PshAI GACNNNNGTC 1 cut(s) 285
Psp5II RGGWCCY 1 cut(s) 688
PspEI GGTNACC 1 cut(s) 391
PspLI CGTACG 1 cut(s) 747
PspN4I GGNNCC 8 cut(s) 70, 90, 191, 282, 336, 474, 689, 790
PspPI GGNCC 3 cut(s) 89, 280, 688
PspPPI RGGWCCY 1 cut(s) 688
PsuI RGATCY 1 cut(s) 189
RsaI GTAC 2 cut(s) 326, 749
RsaNI GTAC 2 cut(s) 325, 748
RseI CAYNNNNRTG 2 cut(s) 389, 846
SacII CCGCGG 1 cut(s) 624
SaqAI TTAA 2 cut(s) 693, 871
SatI GCNGC 3 cut(s) 160, 379, 621
Sau3AI GATC 3 cut(s) 149, 189, 270
Sau96I GGNCC 3 cut(s) 89, 280, 688
ScaI AGTACT 1 cut(s) 326
ScrFI CCNGG 1 cut(s) 854
SetI ASST 3 cut(s) 86, 113, 693
SfaNI GCATC 2 cut(s) 307, 428
Sfr303I CCGCGG 1 cut(s) 624
SgrBI CCGCGG 1 cut(s) 624
SinI GGWCC 3 cut(s) 89, 280, 688
SmiMI CAYNNNNRTG 2 cut(s) 389, 846
SmlI CTYRAG 1 cut(s) 53
SmoI CTYRAG 1 cut(s) 53
Sse9I AATT 5 cut(s) 307, 403, 512, 632, 767
SseBI AGGCCT 1 cut(s) 604
SspMI CTAG 2 cut(s) 477, 509
StuI AGGCCT 1 cut(s) 604
StyD4I CCNGG 1 cut(s) 852
StyI CCWWGG 1 cut(s) 84
TaaI ACNGT 2 cut(s) 73, 206
TaqI TCGA 1 cut(s) 401
TasI AATT 5 cut(s) 307, 403, 512, 632, 767
TatI WGTACW 1 cut(s) 324
TauI GCSGC 3 cut(s) 162, 381, 623
TfiI GAWTC 2 cut(s) 15, 806
Tru1I TTAA 2 cut(s) 693, 871
Tru9I TTAA 2 cut(s) 693, 871
TseFI GTSAC 4 cut(s) 73, 143, 391, 557
Tsp45I GTSAC 4 cut(s) 73, 143, 391, 557
TspDTI ATGAA 4 cut(s) 555, 645, 769, 780
TspGWI ACGGA 1 cut(s) 201
VpaK11BI GGWCC 3 cut(s) 89, 280, 688
XapI RAATTY 2 cut(s) 512, 632
XceI RCATGY 1 cut(s) 550
XmiI GTMKAC 3 cut(s) 38, 678, 717
XmnI GAANNNNTTC 1 cut(s) 632
XspI CTAG 2 cut(s) 477, 509
ZrmI AGTACT 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.