AT5G37160

AAA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000279
Physical Location & Seq
Forward (+)
14705426 .. 14706981
1556 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G37160.1

Sequence Viewer

Length: 1368 bp
ATGGACACTAAAGAGATACGAAAGCTCTTGGAAAAAAACGAGCAAACAAGTCTTTTCGCTAGACTATGTTCTTGGTCACTCAAAGATATCCTCAACGAAGATCTCTCCAAGGAAAAGATAATGACAATACCAGACAGATTTAGTTCCGTTGATGAATACTCTCAGTGTTTTGTTCCTCATTTACTCGAGGAAACACGAACTGAGTTGTTCTCGAGCTTCAGATCTTTATCGAAATCTCCTGTCTCACGAATACTTTCTGTGGAGACAAAAGTGATTGAATATAGTGGAAGGTCATCGATCAAGTGGTTCCATGATATTAAATTAATGGATTATGCAGACGATAAAAACGAAATATATGAGCCAAAGTGTGGAGATATTATTGCACTGAGTCCTCTGAGTTTGACAGAAGAAAGGCCAAGAATCGATGACTTGGATCCTTTACTTCTAGGTTACGTTTTCTCTGTGTATGGTGATTCTAAAATCTCTGTTCACTTCTCTAGATCCATTTCTCAGAGTGAGAAACATACATTTTGCACAGGTGTTTTTCTCATTAATATAACCACAAACACTCGAATTTGGAACGCTTTACACAAGGATGCTGCCGATTCCACTCTGATCCAGAGTGTCCTTCAGGAAGATGCTTCGGCTACAGAACAATGTTTTTCTTGTGAAAATGATGTTGATGGTTCTGATTCTGACCGTGTTGTCGATATAATACGTTCAGCAAAACTGAACTCTTCCCAAGAAGCTGCGATTTTGGGTTTCCTTAAGACAAGGAATTGTAAACACAAGGAGAGTGTGAAACTGATATGGGGACCTCCTGGTACAGGCAAAACAAAGACGGTCGCGACACTTCTCTCTACTCTTATGCAACTAAAGTGCAAAACAGTTGTATGTGCTCCTACAAACACAACTATTGTAGCAGTAGCGTCAAGGTTATTATCTTTATCTAAGGAAACAATTGTGTGTGCTCCAACCAACTCGGCTATCGCAGAAGTAGTATCAAGGTTCGAATTCTCTACCTTATTCTATGGAACTTCTATATTAGAACGTACCACTTACGGTATGGGGAATATAGTTCTATCTGGAAACCGCGAGAGAATGGGAATTACGAGCAATAAAGTTCTTCTCAATGTGTTTTTTAATGACCGCGTTAGTAAACTTGGTAGACTGTTTTTATCTACTTGTGGATGGAAGAAGAGGTTAGAGTCAATCATAGATTTTCTTGAGAACACAGAGACTAAGTACGAGCAACATGTAAATGAACTAGAGCTTGAAAGAATGACAGAAGATGAAAAGAAGAAGGAAGAAGTTGAAGAGAGGACGATGCAAGAAGTTGTAAACATTCCAACATTTGAGAGTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12

Protein Analysis

455

Amino Acids

51.53

Weight (kDa)

5.54

Isoelectric Point (pI)

38.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_11 PF13086 243 - 443 1.5e-14 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000279)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65810 AT1G65810 AT1G65810 AT4G05540 AT5G37140 AT5G37150 AT5G37160 AT5G37165 AT5G52090
fragaria_vesca FvH4_1g27541 FvH4_1g27542 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_3g00170 FvH4_3g00170 FvH4_3g00170 FvH4_5g05930 FvH4_5g05930
malus_domestica MD08G1077700.v1.1 MD08G1077900.v1.1 MD08G1078000.v1.1 MD08G1078100.v1.1 MD08G1078300.v1.1 MD09G1057800.v1.1 MD09G1281400.v1.1 MD11G1222900.v1.1 MD14G1146400.v1.1
prunus_persica Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G202100_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.3G013100_v2.0.a1
pyrus_communis pycom09g18900 pycom09g18910 pycom13g26720 pycom13g26730 pycom13g26760 pycom13g26770
rosa_chinensis RchiOBHm_Chr3g0488001 RchiOBHm_Chr3g0488011 RchiOBHm_Chr3g0488031 RchiOBHm_Chr3g0488061 RchiOBHm_Chr3g0488111 RchiOBHm_Chr3g0488201 RchiOBHm_Chr3g0488211 RchiOBHm_Chr3g0488221 RchiOBHm_Chr3g0488321 RchiOBHm_Chr3g0488431 RchiOBHm_Chr6g0264601 RchiOBHm_Chr6g0264641
rosa_laevigata RLG00000014240 RLG00000014241 RLG00000022910 RLG00000022949 RLG00000030376
rosa_multiflora Rmu_co8075388.1_g000001 Rmu_co8424869.1_g000001 Rmu_co8427783.1_g000001 Rmu_sc0000546.1_g000002 Rmu_sc0000546.1_g000015 Rmu_sc0011052.1_g000014 Rmu_sc0017772.1_g000004
rosa_roxburghii Rroxscaffold_175G00431980 Rroxscaffold_175G00431990 Rroxscaffold_1G00034600 Rroxscaffold_6G00394230 Rroxscaffold_6G00394270 Rroxscaffold_6G00394280 Rroxscaffold_6G00394350 Rroxscaffold_7G00203240 Rroxscaffold_7G00203270 Rroxscaffold_7G00203280 Rroxscaffold_7G00203300 Rroxscaffold_7G00205740
rosa_rugosa Rorug03G0238000 Rorug03G0238100 Rorug03G0238100 Rorug03G0238500 Rorug05G0155400 Rorug05G0356000 Rorug05G0463000 Rorug06G0015200 Rorug06G0015300 Rorug06G0015400 Rorug06G0015500 Rorug06G0015500
rosa_samantha Rh1DG088100 Rh2AG244800 Rh2BG187500 Rh2CG247300 Rh3BG322700 Rh3BG322800 Rh3BG323100 Rh3BG323900 Rh3BG324000 Rh3BG324300 Rh3CG321300 Rh3CG321400 Rh3CG321500 Rh3DG319400 Rh3DG319900 Rh3DG320100 Rh3DG320200 Rh6AG133700 Rh6AG133800 Rh6AG133900 Rh6CG129300 Rh6CG129800 Rh6CG130200 Rh6DG116500 Rh6DG117200 Rh7DG378000
rosa_wichuraiana Rw2G014710 Rw3G025340 Rw3G025350 Rw3G025360 Rw5G020630 Rw6G011590 Rw6G011600 Rw7G041380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 704
AccB7I CCANNNNNTGG 1 cut(s) 368
AccI GTMKAC 1 cut(s) 1168
AccII CGCG 3 cut(s) 848, 1095, 1152
AciI CCGC 2 cut(s) 1093, 1150
AclWI GGATC 4 cut(s) 428, 441, 495, 610
AcsI RAATTY 2 cut(s) 573, 1013
AcuI CTGAAG 2 cut(s) 202, 614
AfaI GTAC 3 cut(s) 826, 1054, 1247
AfiI CCNNNNNNNGG 2 cut(s) 368, 827
AflII CTTAAG 1 cut(s) 767
AflIII ACRYGT 1 cut(s) 1255
AgsI TTSAA 3 cut(s) 278, 1277, 1316
AjnI CCWGG 1 cut(s) 820
AluBI AGCT 4 cut(s) 25, 216, 749, 1273
AluI AGCT 4 cut(s) 25, 216, 749, 1273
Alw21I GWGCWC 2 cut(s) 901, 973
Alw26I GTCTC 3 cut(s) 247, 257, 1232
AlwI GGATC 4 cut(s) 428, 441, 495, 610
Ama87I CYCGRG 2 cut(s) 185, 211
AoxI GGCC 1 cut(s) 413
ApeKI GCWGC 2 cut(s) 599, 749
ApoI RAATTY 2 cut(s) 573, 1013
AseI ATTAAT 2 cut(s) 323, 552
Asp700I GAANNNNTTC 1 cut(s) 253
AspS9I GGNCC 1 cut(s) 815
AsuHPI GGTGA 1 cut(s) 482
AsuII TTCGAA 1 cut(s) 1011
AvaI CYCGRG 2 cut(s) 185, 211
AvaII GGWCC 1 cut(s) 815
BamHI GGATCC 1 cut(s) 433
Bbv12I GWGCWC 2 cut(s) 901, 973
BbvI GCAGC 2 cut(s) 586, 736
BccI CCATC 2 cut(s) 677, 1185
BciT130I CCWGG 1 cut(s) 822
BcoDI GTCTC 3 cut(s) 247, 257, 1232
BfaI CTAG 4 cut(s) 60, 446, 498, 1268
BfmI CTRYAG 1 cut(s) 648
BfrI CTTAAG 1 cut(s) 767
BglII AGATCT 2 cut(s) 100, 221
BisI GCNGC 2 cut(s) 600, 750
BlsI GCNGC 2 cut(s) 601, 751
Bme1390I CCNGG 1 cut(s) 822
Bme18I GGWCC 1 cut(s) 815
BmeT110I CYCGRG 2 cut(s) 185, 211
BmgT120I GGNCC 1 cut(s) 815
BmiI GGNNCC 3 cut(s) 308, 435, 816
BmrFI CCNGG 1 cut(s) 822
BmsI GCATC 3 cut(s) 586, 628, 1317
BplI GAGNNNNNCTC 2 cut(s) 194, 226
Bpu14I TTCGAA 1 cut(s) 1011
BpuEI CTTGAG 1 cut(s) 1247
Bsa29I ATCGAT 2 cut(s) 296, 423
BsaBI GATNNNNATC 1 cut(s) 226
BsaJI CCNNGG 1 cut(s) 108
Bsc4I CCNNNNNNNGG 2 cut(s) 368, 827
Bse8I GATNNNNATC 1 cut(s) 226
BseBI CCWGG 1 cut(s) 822
BseCI ATCGAT 2 cut(s) 296, 423
BseDI CCNNGG 1 cut(s) 108
BseGI GGATG 2 cut(s) 601, 1196
BseJI GATNNNNATC 1 cut(s) 226
BseLI CCNNNNNNNGG 2 cut(s) 368, 827
BseMII CTCAG 5 cut(s) 176, 192, 377, 386, 524
BseXI GCAGC 2 cut(s) 586, 736
Bsh1236I CGCG 3 cut(s) 848, 1095, 1152
Bsh1285I CGRYCG 1 cut(s) 846
BshFI GGCC 1 cut(s) 415
BshVI ATCGAT 2 cut(s) 296, 423
BsiEI CGRYCG 1 cut(s) 846
BsiHKAI GWGCWC 2 cut(s) 901, 973
BsiHKCI CYCGRG 2 cut(s) 185, 211
BslFI GGGAC 1 cut(s) 828
BslI CCNNNNNNNGG 2 cut(s) 368, 827
BsmAI GTCTC 3 cut(s) 247, 257, 1232
BsmFI GGGAC 1 cut(s) 828
BsnI GGCC 1 cut(s) 415
BsoBI CYCGRG 2 cut(s) 185, 211
Bsp119I TTCGAA 1 cut(s) 1011
Bsp1286I GDGCHC 2 cut(s) 901, 973
Bsp143I GATC 6 cut(s) 100, 221, 297, 433, 500, 615
Bsp68I TCGCGA 1 cut(s) 848
BspACI CCGC 2 cut(s) 1093, 1150
BspANI GGCC 1 cut(s) 415
BspCNI CTCAG 5 cut(s) 175, 193, 378, 387, 523
BspDI ATCGAT 2 cut(s) 296, 423
BspFNI CGCG 3 cut(s) 848, 1095, 1152
BspLI GGNNCC 3 cut(s) 308, 435, 816
BspPI GGATC 4 cut(s) 428, 441, 495, 610
BspT104I TTCGAA 1 cut(s) 1011
BspTI CTTAAG 1 cut(s) 767
BssECI CCNNGG 1 cut(s) 108
BssMI GATC 6 cut(s) 100, 221, 297, 433, 500, 615
BssT1I CCWWGG 1 cut(s) 108
Bst2UI CCWGG 1 cut(s) 822
Bst4CI ACNGT 5 cut(s) 701, 844, 889, 1064, 1173
Bst6I CTCTTC 3 cut(s) 742, 1193, 1311
BstAFI CTTAAG 1 cut(s) 767
BstBI TTCGAA 1 cut(s) 1011
BstDEI CTNAG 7 cut(s) 162, 201, 386, 395, 510, 951, 1242
BstENI CCTNNNNNAGG 1 cut(s) 825
BstF5I GGATG 2 cut(s) 601, 1196
BstFNI CGCG 3 cut(s) 848, 1095, 1152
BstKTI GATC 6 cut(s) 103, 224, 300, 436, 503, 618
BstMAI GTCTC 3 cut(s) 247, 257, 1232
BstMBI GATC 6 cut(s) 100, 221, 297, 433, 500, 615
BstMCI CGRYCG 1 cut(s) 846
BstNI CCWGG 1 cut(s) 822
BstNSI RCATGY 1 cut(s) 1259
BstSCI CCNGG 1 cut(s) 820
BstSFI CTRYAG 1 cut(s) 648
BstUI CGCG 3 cut(s) 848, 1095, 1152
BstV1I GCAGC 2 cut(s) 586, 736
BstX2I RGATCY 4 cut(s) 100, 221, 433, 500
BstYI RGATCY 4 cut(s) 100, 221, 433, 500
Bsu15I ATCGAT 2 cut(s) 296, 423
BsuRI GGCC 1 cut(s) 415
BsuTUI ATCGAT 2 cut(s) 296, 423
BtsCI GGATG 2 cut(s) 601, 1196
BtsIMutI CAGTG 2 cut(s) 170, 383
BtuMI TCGCGA 1 cut(s) 848
Cfr13I GGNCC 1 cut(s) 815
ClaI ATCGAT 2 cut(s) 296, 423
CseI GACGC 1 cut(s) 918
Csp6I GTAC 3 cut(s) 825, 1053, 1246
CviAII CATG 2 cut(s) 311, 1256
CviJI RGCY 8 cut(s) 25, 216, 361, 415, 647, 749, 986, 1273
CviKI_1 RGCY 8 cut(s) 25, 216, 361, 415, 647, 749, 986, 1273
CviQI GTAC 3 cut(s) 825, 1053, 1246
DdeI CTNAG 7 cut(s) 162, 201, 386, 395, 510, 951, 1242
DpnI GATC 6 cut(s) 102, 223, 299, 435, 502, 617
DpnII GATC 6 cut(s) 100, 221, 297, 433, 500, 615
DrdI GACNNNNNNGTC 1 cut(s) 704
DseDI GACNNNNNNGTC 1 cut(s) 704
Eam1104I CTCTTC 3 cut(s) 742, 1193, 1311
EarI CTCTTC 3 cut(s) 742, 1193, 1311
Eco130I CCWWGG 1 cut(s) 108
Eco32I GATATC 1 cut(s) 88
Eco47I GGWCC 1 cut(s) 815
Eco57I CTGAAG 2 cut(s) 202, 614
Eco88I CYCGRG 2 cut(s) 185, 211
EcoNI CCTNNNNNAGG 1 cut(s) 825
EcoO109I RGGNCCY 1 cut(s) 815
EcoRI GAATTC 1 cut(s) 1013
EcoRII CCWGG 1 cut(s) 820
EcoRV GATATC 1 cut(s) 88
EcoT14I CCWWGG 1 cut(s) 108
ErhI CCWWGG 1 cut(s) 108
FaeI CATG 2 cut(s) 314, 1259
FaqI GGGAC 1 cut(s) 828
FatI CATG 2 cut(s) 310, 1255
FblI GTMKAC 1 cut(s) 1168
Fnu4HI GCNGC 2 cut(s) 600, 750
FokI GGATG 2 cut(s) 608, 1203
Fsp4HI GCNGC 2 cut(s) 600, 750
FspBI CTAG 4 cut(s) 60, 446, 498, 1268
GluI GCNGC 2 cut(s) 600, 750
HaeIII GGCC 1 cut(s) 415
HgaI GACGC 1 cut(s) 918
Hin1II CATG 2 cut(s) 314, 1259
HinfI GANTC 6 cut(s) 388, 420, 473, 605, 692, 1208
HphI GGTGA 1 cut(s) 482
Hpy166II GTNNAC 5 cut(s) 490, 785, 1160, 1169, 1342
Hpy188I TCNGA 6 cut(s) 221, 396, 513, 615, 691, 697
Hpy188III TCNNGA 8 cut(s) 211, 246, 498, 619, 632, 847, 1086, 1226
Hpy8I GTNNAC 5 cut(s) 490, 785, 1160, 1169, 1342
HpyAV CCTTC 3 cut(s) 282, 638, 1297
HpyCH4III ACNGT 5 cut(s) 701, 844, 889, 1064, 1173
HpyCH4IV ACGT 3 cut(s) 453, 718, 1051
HpyCH4V TGCA 6 cut(s) 335, 383, 534, 871, 882, 1330
HpyF3I CTNAG 7 cut(s) 162, 201, 386, 395, 510, 951, 1242
HpySE526I ACGT 3 cut(s) 453, 718, 1051
Hsp92II CATG 2 cut(s) 314, 1259
Kzo9I GATC 6 cut(s) 100, 221, 297, 433, 500, 615
LmnI GCTCC 2 cut(s) 904, 976
LpnPI CCDG 9 cut(s) 144, 252, 522, 617, 632, 807, 813, 834, 1071
Lsp1109I GCAGC 2 cut(s) 586, 736
LweI GCATC 3 cut(s) 586, 628, 1317
MaeI CTAG 4 cut(s) 60, 446, 498, 1268
MaeII ACGT 3 cut(s) 453, 718, 1051
MaeIII GTNAC 2 cut(s) 75, 449
MalI GATC 6 cut(s) 102, 223, 299, 435, 502, 617
MboI GATC 6 cut(s) 100, 221, 297, 433, 500, 615
MfeI CAATTG 1 cut(s) 960
MflI RGATCY 4 cut(s) 100, 221, 433, 500
MhlI GDGCHC 2 cut(s) 901, 973
MluCI AATT 6 cut(s) 320, 573, 778, 960, 1013, 1107
MlyI GAGTC 2 cut(s) 397, 1217
MmeI TCCRAC 1 cut(s) 998
MnlI CCTC 7 cut(s) 101, 181, 186, 402, 828, 1194, 1314
MroXI GAANNNNTTC 1 cut(s) 253
MseI TTAA 5 cut(s) 318, 323, 552, 768, 1143
MslI CAYNNNNRTG 2 cut(s) 594, 1260
MspCI CTTAAG 1 cut(s) 767
MspR9I CCNGG 1 cut(s) 822
MunI CAATTG 1 cut(s) 960
MvaI CCWGG 1 cut(s) 822
MvnI CGCG 3 cut(s) 848, 1095, 1152
NdeII GATC 6 cut(s) 100, 221, 297, 433, 500, 615
NlaIII CATG 2 cut(s) 314, 1259
NlaIV GGNNCC 3 cut(s) 308, 435, 816
NmeAIII GCCGAG 1 cut(s) 962
NmuCI GTSAC 1 cut(s) 75
NruI TCGCGA 1 cut(s) 848
NspI RCATGY 1 cut(s) 1259
NspV TTCGAA 1 cut(s) 1011
PaeR7I CTCGAG 2 cut(s) 185, 211
PciI ACATGT 1 cut(s) 1255
PcsI WCGNNNNNNNCGW 1 cut(s) 345
PdmI GAANNNNTTC 1 cut(s) 253
PfeI GAWTC 4 cut(s) 420, 473, 605, 692
PflMI CCANNNNNTGG 1 cut(s) 368
PkrI GCNGC 2 cut(s) 601, 751
PleI GAGTC 2 cut(s) 396, 1216
PpsI GAGTC 2 cut(s) 396, 1216
PpuMI RGGWCCY 1 cut(s) 815
PscI ACATGT 1 cut(s) 1255
PshBI ATTAAT 2 cut(s) 323, 552
Psp5II RGGWCCY 1 cut(s) 815
Psp6I CCWGG 1 cut(s) 820
PspGI CCWGG 1 cut(s) 820
PspN4I GGNNCC 3 cut(s) 308, 435, 816
PspPI GGNCC 1 cut(s) 815
PspPPI RGGWCCY 1 cut(s) 815
PspXI VCTCGAGB 1 cut(s) 185
PsuI RGATCY 4 cut(s) 100, 221, 433, 500
RruI TCGCGA 1 cut(s) 848
RsaI GTAC 3 cut(s) 826, 1054, 1247
RsaNI GTAC 3 cut(s) 825, 1053, 1246
RseI CAYNNNNRTG 2 cut(s) 594, 1260
SaqAI TTAA 5 cut(s) 318, 323, 552, 768, 1143
SatI GCNGC 2 cut(s) 600, 750
Sau3AI GATC 6 cut(s) 100, 221, 297, 433, 500, 615
Sau96I GGNCC 1 cut(s) 815
SchI GAGTC 2 cut(s) 397, 1217
ScrFI CCNGG 1 cut(s) 822
SduI GDGCHC 2 cut(s) 901, 973
SfaNI GCATC 3 cut(s) 586, 628, 1317
SfcI CTRYAG 1 cut(s) 648
Sfr274I CTCGAG 2 cut(s) 185, 211
SfuI TTCGAA 1 cut(s) 1011
SinI GGWCC 1 cut(s) 815
SlaI CTCGAG 2 cut(s) 185, 211
SmiMI CAYNNNNRTG 2 cut(s) 594, 1260
SmlI CTYRAG 4 cut(s) 185, 211, 767, 1226
SmoI CTYRAG 4 cut(s) 185, 211, 767, 1226
Sse9I AATT 6 cut(s) 320, 573, 778, 960, 1013, 1107
SsiI CCGC 2 cut(s) 1093, 1150
SspMI CTAG 4 cut(s) 60, 446, 498, 1268
StyD4I CCNGG 1 cut(s) 820
StyI CCWWGG 1 cut(s) 108
TaaI ACNGT 5 cut(s) 701, 844, 889, 1064, 1173
TaiI ACGT 3 cut(s) 456, 721, 1054
TaqI TCGA 8 cut(s) 186, 212, 230, 296, 423, 571, 708, 1011
TasI AATT 6 cut(s) 320, 573, 778, 960, 1013, 1107
TfiI GAWTC 4 cut(s) 420, 473, 605, 692
Tru1I TTAA 5 cut(s) 318, 323, 552, 768, 1143
Tru9I TTAA 5 cut(s) 318, 323, 552, 768, 1143
TscAI CASTG 2 cut(s) 170, 390
TseFI GTSAC 1 cut(s) 75
TseI GCWGC 2 cut(s) 599, 749
Tsp45I GTSAC 1 cut(s) 75
TspDTI ATGAA 3 cut(s) 168, 1278, 1308
TspGWI ACGGA 1 cut(s) 136
TspRI CASTG 2 cut(s) 170, 390
Van91I CCANNNNNTGG 1 cut(s) 368
Vha464I CTTAAG 1 cut(s) 767
VpaK11BI GGWCC 1 cut(s) 815
VspI ATTAAT 2 cut(s) 323, 552
XagI CCTNNNNNAGG 1 cut(s) 825
XapI RAATTY 2 cut(s) 573, 1013
XbaI TCTAGA 1 cut(s) 497
XceI RCATGY 1 cut(s) 1259
XcmI CCANNNNNNNNNTGG 1 cut(s) 1063
XhoI CTCGAG 2 cut(s) 185, 211
XmiI GTMKAC 1 cut(s) 1168
XmnI GAANNNNTTC 1 cut(s) 253
XspI CTAG 4 cut(s) 60, 446, 498, 1268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.