MD09G1281400.v1.1

AAA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000279
Physical Location & Seq
Reverse (-)
35849485 .. 35855682
6198 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1281400.v1.1.491

Sequence Viewer

Length: 3453 bp
ATGGAGAAGACTAACGTGAGCAACAAGAAAGAAGTTACAGACAAAAAAGCCTTCCTCGATCTGGTTTTCTCTTGGTCTCTCAGCGATGTTCTCAACAACAAACTTTACAAAAATCAGGTGCCTAGGATTCCTGAGACCTTCTTGAATGTGACGAGTTATTTGAAATCATTTGTTCCTTCACTCATCGAGGAAACACATGCTGATTTACTCTCAAATATGATCACGGAACTGTCGCATGCACCTACTTGTGAAATATTGACTCTTGAATGTTCAAAAGGTCATAAAAATCCCCAAAATTTGGTCTATGATATTGCATATAAGATAGATACAGAAGCGGATCAGAAGCACAATGGATTAATGTACGAGCCACAGGTTGGAGATCTCATTGCCTTGACGGATGTTAAACCGAAATGTATTGATGATTTGAACAGGTTCCCAAGACACTATGTTGCTGCTTATGTTAGTAAAGTACCTGATCCGGAAGAATTTCCTGATGCTCATGGGTTCACAATACTCTCATCCAAGCCTATCGATTATGGAGAACAAGACGGACAAAAGAGCAAGAGACAAAAACATTTTGCAGTTTATCTCATGAACATGACAACAAATATTCGCGTGTGGTGTGCTTTGAACTCAAAAGAGGGAAACACAAATATTATTAAGAAAGTTCTGCAACCCAATTCATATGTTGTGAGTTCTTGTACTACTTGCTCTCCCATACAAAGGTGCTCTCCGGCCCTTTCTACCATTTGGCCCACTATTTCCTCTCACAATCTAAATGACTCCCAAGAAGCTGCAGTTTTAAACTTTATTGGTTTGAGTAAATGCCATCATCAGAATGATGTCAAATTAATTTGGGGTCCTCCAGGTACTGGGAAGACAAAGACAGTCAGTCTGTCTCTCTTTGCCCTCTTTATGCTCAAGTGCAGAACACTAACATGTGCTCCTACCAATATTGCGGTGTTAGAAGTTGCAGCCAGGCTCAGGAGTTTGTTTAATCGGTCACTTGAGTTTGGAAAGTACGGACTTGGAGATATAGTTCTCTTTGGGAACAAGAAGCGAATGAAGGTTGATGATAAGGCTGACCTTCTTGAGGTATTTCTTGATCATCGTATTAAAATCCTGATCAAGTGTTTGGCGCCCTTGTCAGGATGGAAGCATCTGTTAAGATCAATGATACGTCTACTTGAGGAACCAGAAGAAGAGTACTCTTTGTATTTGCAAAAGAAAGCAGAAGAACACAAAGAGAGTGCTCAAGTAAATGAGAAGAAAAACATGAATAGAGCAGAAAAACACAAAGAGAGTGCTCAAGGAAATCAGAAGAAAAACACGAAGAGAGCAGAAAAACACAAAGAGAGTGCTCAAGGAAATCAGAAGAAAAACATGAAGAGAGCAGAAAAACACAAAGAGAGTGCTCAAGGAAATGGGAAGGGTACTAAAGGAAACACCGAAAGTGTAAAAGAGGATGATCCATTGACGTTTGAGGAGTTTGTGAAGAAAGAATTTGATTCAATTTTTGAGCCACTGAAGATTTGTTTGGTAAATTTGCACACTCACTTACCAACTTCTTGCATTTCACTGAAGGTGGTGAAGGACATGGTTGAAGCTTTGGGTTTGCTCAAGTCCTTTAAATCTTCCATGCATAGTATCAGTGTTGCTAAGGAAGGTTTAAATTTAGTCCTAAAAGATTTCAAAGTTTCAGGAAGCATTGCCTGTTGCTTTACACAGTTGGGAAAAAAGTGTGTTTGTAAACTGAAGCTGCTTCCTCAGGAATTCAGTGTCCCAGAAATTATTAGCCCTCCTTCAATAAGAAGTTTCTGCTTGCAAAATGCTTGCTTAATATTTTGTACTGCATCTTCTTCTGCCAAATTGCACAACGCATCAGCGAAGAGACCACTGGAATTGTTAGTCGTTGATGAAGCTGCTCAGCTTAAAGAATGTGAGTCCGCAATTCCTTTACAACTGCCTGGTCTCCGCCATGCTATTCTAATAGGAGATGAGCGGCAACTCCCTGCTATGGTTATAAGCGAGAAAGCGGCAGCGGCTGAGTTTGGAAGAAGTTTGTTCGAAAGGATGACAATGTTGGGATACGGGAAGCATCTTCTCAATATCCAATATAGGATGCATCCATCCATCAGCTTATTTCCGAAAATGGAGTTTTATAACAATCAGTTATTAGATGGTCCGAATGTCACTGAAAGAAGCTATGAGAGGCGCTTCCTTGAGGGAAAGATGTACCAATCCTACTCCTTCATAAATGTAGCCAATGGAAAAGATGAATTTGATCATGGTCATAGTCGGAAAAATATGTTGGAGGTTGCTGTGGTCTCTGAGATAGTTGCAAGACTTTACAAAGAATTCATCGGCACAAAGAAGAAGGTTAGTATTGGAGTCATATCACCATACAAGGCTCAAGTTTATGCAATTCAAGAGAGGGTGAAAAAATACAGTAAAAATTGTGACGCTCGATTCTCCGTAAATGTGCGGTCTGTTGATGGGTTCCAAGGTGGTGAAGAGGATGTGATAATTATCTCCACTGTCAGATGTAATGTGAATGGTTCAATTGGTTTCTTGTCCAACCGTCAAAGGGCAAATGTTGCACTTACACGTGCACGGTATTGTCTTTGGATATTGGGGAGTGCGTCGACCTTGGTTAACAGTGACTCTATCTGGACGAAGCTAGTACTTGATGCCAAGGGACGCAATTGTTTTCACAATGCTGACGAGGACAAGAACTTGGCACAGGCTATTGCCATTGCCCTGCTGGAGCTTGACCAATTTCATGGTCTACTTAATATTGAATCTCTGCTGTTCAAAAATGCAAGATGGAAGGTTTGCTTCACTGATCAATTTGAGAAATCCATAGCAAAGATAAAAGACAATTGGGTTCAACGAGAAGTGTTTAATCTACTAACGAAGCTTTCAAGTGGATGGCGTCAAGCACACAAGGCTAAGGGAATGATAGTTCATGACGGGACTTCTTCTCAACTGTTAGAGACGTATAAAGTCAATAGGCTGCTGAATCTCATTTGGACTGTGGATATTATTCAAGAGAATTCAGATTACATTCAGGTGATGAAGGTTTGGGCTATCGTGACATCTTCAGATATACCTAGACTAGCAAAGCGTCTTGACGTCATTTTTGGGAGTTATACAGTGGATACGATGAATCGTTGCAAACACAAATGTATTGATAAGGGCACTGCTATTCCAATGAGATGGCCGGTGGATTCTAGTAGTTACCATGAAGCTGATCCTGTGGAGTTCCTTTCAAAGCCGCTATCGTCCCTCGGTCTAACAGATAAACCTGAAACATCAACATCAGCTTCTGGGGAGGCTATCAAAACAGAGAAACAGTCTACACAACCCAAAAGCCGTTTGAAAAGTCATGCTAAACAAAGAAGGCAATCACCGAACGTAGAGGTTGGAGTCTGGAGACCGTGTGTTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12

Protein Analysis

1151

Amino Acids

129.67

Weight (kDa)

9.11

Isoelectric Point (pI)

40.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 81 - 213 4.3e-12 Domain of unknown function (DUF6469)
AAA_11 PF13086 258 - 468 9.8e-18 AAA domain
AAA_11 PF13086 603 - 677 1e-15 AAA domain
AAA_12 PF13087 685 - 881 9.1e-56 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000279)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65810 AT1G65810 AT1G65810 AT4G05540 AT5G37140 AT5G37150 AT5G37160 AT5G37165 AT5G52090
fragaria_vesca FvH4_1g27541 FvH4_1g27542 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_3g00170 FvH4_3g00170 FvH4_3g00170 FvH4_5g05930 FvH4_5g05930
malus_domestica MD08G1077700.v1.1 MD08G1077900.v1.1 MD08G1078000.v1.1 MD08G1078100.v1.1 MD08G1078300.v1.1 MD09G1057800.v1.1 MD09G1281400.v1.1 MD11G1222900.v1.1 MD14G1146400.v1.1
prunus_persica Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G202100_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.3G013100_v2.0.a1
pyrus_communis pycom09g18900 pycom09g18910 pycom13g26720 pycom13g26730 pycom13g26760 pycom13g26770
rosa_chinensis RchiOBHm_Chr3g0488001 RchiOBHm_Chr3g0488011 RchiOBHm_Chr3g0488031 RchiOBHm_Chr3g0488061 RchiOBHm_Chr3g0488111 RchiOBHm_Chr3g0488201 RchiOBHm_Chr3g0488211 RchiOBHm_Chr3g0488221 RchiOBHm_Chr3g0488321 RchiOBHm_Chr3g0488431 RchiOBHm_Chr6g0264601 RchiOBHm_Chr6g0264641
rosa_laevigata RLG00000014240 RLG00000014241 RLG00000022910 RLG00000022949 RLG00000030376
rosa_multiflora Rmu_co8075388.1_g000001 Rmu_co8424869.1_g000001 Rmu_co8427783.1_g000001 Rmu_sc0000546.1_g000002 Rmu_sc0000546.1_g000015 Rmu_sc0011052.1_g000014 Rmu_sc0017772.1_g000004
rosa_roxburghii Rroxscaffold_175G00431980 Rroxscaffold_175G00431990 Rroxscaffold_1G00034600 Rroxscaffold_6G00394230 Rroxscaffold_6G00394270 Rroxscaffold_6G00394280 Rroxscaffold_6G00394350 Rroxscaffold_7G00203240 Rroxscaffold_7G00203270 Rroxscaffold_7G00203280 Rroxscaffold_7G00203300 Rroxscaffold_7G00205740
rosa_rugosa Rorug03G0238000 Rorug03G0238100 Rorug03G0238100 Rorug03G0238500 Rorug05G0155400 Rorug05G0356000 Rorug05G0463000 Rorug06G0015200 Rorug06G0015300 Rorug06G0015400 Rorug06G0015500 Rorug06G0015500
rosa_samantha Rh1DG088100 Rh2AG244800 Rh2BG187500 Rh2CG247300 Rh3BG322700 Rh3BG322800 Rh3BG323100 Rh3BG323900 Rh3BG324000 Rh3BG324300 Rh3CG321300 Rh3CG321400 Rh3CG321500 Rh3DG319400 Rh3DG319900 Rh3DG320100 Rh3DG320200 Rh6AG133700 Rh6AG133800 Rh6AG133900 Rh6CG129300 Rh6CG129800 Rh6CG130200 Rh6DG116500 Rh6DG117200 Rh7DG378000
rosa_wichuraiana Rw2G014710 Rw3G025340 Rw3G025350 Rw3G025360 Rw5G020630 Rw6G011590 Rw6G011600 Rw7G041380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2024, 2163
AatII GACGTC 1 cut(s) 3138
AccB1I GGYRCC 2 cut(s) 118, 1138
AccB7I CCANNNNNTGG 3 cut(s) 298, 374, 872
AccBSI CCGCTC 1 cut(s) 2002
AccI GTMKAC 4 cut(s) 1183, 2645, 2788, 3359
AccII CGCG 1 cut(s) 615
AccIII TCCGGA 1 cut(s) 478
AciI CCGC 9 cut(s) 335, 959, 1947, 1975, 2002, 2036, 2042, 2485, 3278
AclWI GGATC 4 cut(s) 345, 470, 1463, 3248
AcoI YGGCCR 1 cut(s) 3221
AcsI RAATTY 9 cut(s) 295, 485, 1502, 1543, 1672, 1772, 2279, 2357, 3055
AcuI CTGAAG 4 cut(s) 1547, 1601, 1775, 3087
AcvI CACGTG 1 cut(s) 2609
AcyI GRCGYC 3 cut(s) 1139, 2935, 3135
AflIII ACRYGT 2 cut(s) 938, 2606
AhdI GACNNNNNGTC 1 cut(s) 891
AjnI CCWGG 3 cut(s) 865, 977, 1966
AjuI GAANNNNNNNTTGG 4 cut(s) 1520, 1552, 2294, 2326
AloI GAACNNNNNNTCC 4 cut(s) 1023, 1055, 2949, 2981
Alw21I GWGCWC 7 cut(s) 731, 946, 1255, 1309, 1363, 1417, 2614
Alw26I GTCTC 9 cut(s) 81, 128, 559, 903, 1885, 1976, 2332, 2990, 3430
Alw44I GTGCAC 1 cut(s) 2610
AlwI GGATC 4 cut(s) 345, 470, 1463, 3248
AlwNI CAGNNNCTG 3 cut(s) 872, 2045, 3329
Aor13HI TCCGGA 1 cut(s) 478
AoxI GGCC 3 cut(s) 735, 752, 3221
ApaLI GTGCAC 1 cut(s) 2610
ApeKI GCWGC 7 cut(s) 452, 794, 974, 1759, 1922, 2039, 3016
ApoI RAATTY 9 cut(s) 295, 485, 1502, 1543, 1672, 1772, 2279, 2357, 3055
ArsI GACNNNNNNTTYG 4 cut(s) 142, 174, 3125, 3157
AseI ATTAAT 2 cut(s) 356, 851
Asp700I GAANNNNTTC 2 cut(s) 431, 486
AspA2I CCTAGG 1 cut(s) 122
AspLEI GCGC 2 cut(s) 1141, 2217
AspS9I GGNCC 4 cut(s) 736, 753, 860, 2183
AsuHPI GGTGA 6 cut(s) 1600, 2391, 2449, 2522, 3085, 3402
AsuII TTCGAA 1 cut(s) 2067
AvaII GGWCC 2 cut(s) 860, 2183
AvrII CCTAGG 1 cut(s) 122
AxyI CCTNAGG 1 cut(s) 1767
BaeGI GKGCMC 2 cut(s) 2614, 3203
BanI GGYRCC 2 cut(s) 118, 1138
BarI GAAGNNNNNNTAC 2 cut(s) 1840, 1872
BbrPI CACGTG 1 cut(s) 2609
BbsI GAAGAC 2 cut(s) 14, 884
Bbv12I GWGCWC 7 cut(s) 731, 946, 1255, 1309, 1363, 1417, 2614
BbvI GCAGC 7 cut(s) 439, 781, 986, 1746, 1909, 2051, 3003
BccI CCATC 9 cut(s) 837, 1146, 2137, 2141, 2174, 2489, 2820, 2925, 3213
BceAI ACGGC 1 cut(s) 3360
BciT130I CCWGG 3 cut(s) 867, 979, 1968
BciVI GTATCC 2 cut(s) 2081, 3154
BclI TGATCA 5 cut(s) 219, 1105, 1125, 2284, 2845
BcoDI GTCTC 9 cut(s) 81, 128, 559, 903, 1885, 1976, 2332, 2990, 3430
BfaI CTAG 5 cut(s) 123, 2681, 3114, 3119, 3234
BfmI CTRYAG 1 cut(s) 795
BfoI RGCGCY 2 cut(s) 1142, 2218
BfuI GTATCC 2 cut(s) 2081, 3154
BglII AGATCT 1 cut(s) 379
BlnI CCTAGG 1 cut(s) 122
BlpI GCTNAGC 1 cut(s) 1926
BmcAI AGTACT 2 cut(s) 1208, 2685
Bme1390I CCNGG 3 cut(s) 867, 979, 1968
Bme18I GGWCC 2 cut(s) 860, 2183
BmeRI GACNNNNNGTC 1 cut(s) 891
BmgT120I GGNCC 4 cut(s) 736, 753, 860, 2183
BmiI GGNNCC 6 cut(s) 120, 434, 861, 1140, 1194, 2501
BmrFI CCNGG 3 cut(s) 867, 979, 1968
BmrI ACTGGG 1 cut(s) 882
BmsI GCATC 8 cut(s) 484, 1168, 1862, 1889, 2107, 2112, 2134, 2680
BmuI ACTGGG 1 cut(s) 882
BpiI GAAGAC 2 cut(s) 14, 884
BpmI CTGGAG 2 cut(s) 849, 2786
Bpu10I CCTNAGC 3 cut(s) 983, 1659, 2952
Bpu1102I GCTNAGC 1 cut(s) 1926
Bpu14I TTCGAA 1 cut(s) 2067
Bsa29I ATCGAT 1 cut(s) 531
BsaAI YACGTR 1 cut(s) 2609
BsaBI GATNNNNATC 1 cut(s) 2528
BsaHI GRCGYC 3 cut(s) 1139, 2935, 3135
BsaI GGTCTC 6 cut(s) 81, 128, 1885, 1976, 2332, 3430
BsaJI CCNNGG 5 cut(s) 122, 2503, 2649, 2694, 3289
BsaWI WCCGGW 1 cut(s) 478
BsaXI ACNNNNNCTCC 7 cut(s) 697, 727, 928, 958, 1023, 1053, 3427
Bse118I RCCGGY 1 cut(s) 3223
Bse1I ACTGG 2 cut(s) 877, 1902
Bse21I CCTNAGG 1 cut(s) 1767
Bse3DI GCAATG 3 cut(s) 384, 1707, 2754
Bse8I GATNNNNATC 1 cut(s) 2528
BseAI TCCGGA 1 cut(s) 478
BseBI CCWGG 3 cut(s) 867, 979, 1968
BseCI ATCGAT 1 cut(s) 531
BseDI CCNNGG 5 cut(s) 122, 2503, 2649, 2694, 3289
BseJI GATNNNNATC 1 cut(s) 2528
BseMI GCAATG 3 cut(s) 384, 1707, 2754
BseMII CTCAG 7 cut(s) 94, 123, 997, 1781, 1940, 2037, 2322
BseNI ACTGG 2 cut(s) 877, 1902
BseRI GAGGAG 1 cut(s) 1499
BseSI GKGCMC 2 cut(s) 2614, 3203
BseXI GCAGC 7 cut(s) 439, 781, 986, 1746, 1909, 2051, 3003
BsgI GTGCAG 1 cut(s) 946
Bsh1236I CGCG 1 cut(s) 615
BshFI GGCC 3 cut(s) 737, 754, 3223
BshNI GGYRCC 2 cut(s) 118, 1138
BshVI ATCGAT 1 cut(s) 531
BsiHKAI GWGCWC 7 cut(s) 731, 946, 1255, 1309, 1363, 1417, 2614
BsiSI CCGG 3 cut(s) 479, 734, 3224
BslFI GGGAC 4 cut(s) 1766, 2712, 2989, 3271
BsmAI GTCTC 9 cut(s) 81, 128, 559, 903, 1885, 1976, 2332, 2990, 3430
BsmBI CGTCTC 1 cut(s) 2990
BsmFI GGGAC 4 cut(s) 1766, 2712, 2989, 3271
BsnI GGCC 3 cut(s) 737, 754, 3223
Bso31I GGTCTC 6 cut(s) 81, 128, 1885, 1976, 2332, 3430
Bsp119I TTCGAA 1 cut(s) 2067
Bsp1286I GDGCHC 8 cut(s) 731, 946, 1255, 1309, 1363, 1417, 2614, 3203
Bsp13I TCCGGA 1 cut(s) 478
Bsp1720I GCTNAGC 1 cut(s) 1926
BspACI CCGC 9 cut(s) 335, 959, 1947, 1975, 2002, 2036, 2042, 2485, 3278
BspANI GGCC 3 cut(s) 737, 754, 3223
BspCNI CTCAG 7 cut(s) 93, 124, 996, 1780, 1939, 2038, 2323
BspDI ATCGAT 1 cut(s) 531
BspEI TCCGGA 1 cut(s) 478
BspFNI CGCG 1 cut(s) 615
BspHI TCATGA 2 cut(s) 591, 2968
BspLI GGNNCC 6 cut(s) 120, 434, 861, 1140, 1194, 2501
BspMAI CTGCAG 1 cut(s) 799
BspPI GGATC 4 cut(s) 345, 470, 1463, 3248
BspT104I TTCGAA 1 cut(s) 2067
BspT107I GGYRCC 2 cut(s) 118, 1138
BspTNI GGTCTC 6 cut(s) 81, 128, 1885, 1976, 2332, 3430
BsrBI CCGCTC 1 cut(s) 2002
BsrDI GCAATG 3 cut(s) 384, 1707, 2754
BsrFI RCCGGY 1 cut(s) 3223
BsrI ACTGG 2 cut(s) 877, 1902
BssAI RCCGGY 1 cut(s) 3223
BssECI CCNNGG 5 cut(s) 122, 2503, 2649, 2694, 3289
BssNI GRCGYC 3 cut(s) 1139, 2935, 3135
BssT1I CCWWGG 4 cut(s) 122, 2503, 2649, 2694
Bst2UI CCWGG 3 cut(s) 867, 979, 1968
Bst6I CTCTTC 5 cut(s) 1197, 1328, 1382, 1883, 2508
BstACI GRCGYC 3 cut(s) 1139, 2935, 3135
BstAPI GCANNNNNTGC 1 cut(s) 2597
BstBAI YACGTR 1 cut(s) 2609
BstBI TTCGAA 1 cut(s) 2067
BstC8I GCNNGC 3 cut(s) 237, 1823, 1834
BstDEI CTNAG 9 cut(s) 80, 132, 983, 1659, 1767, 1926, 2046, 2331, 2952
BstENI CCTNNNNNAGG 1 cut(s) 1091
BstFNI CGCG 1 cut(s) 615
BstH2I RGCGCY 2 cut(s) 1142, 2218
BstHHI GCGC 2 cut(s) 1141, 2217
BstMAI GTCTC 9 cut(s) 81, 128, 559, 903, 1885, 1976, 2332, 2990, 3430
BstMWI GCNNNNNNNGC 3 cut(s) 2042, 2597, 2948
BstNI CCWGG 3 cut(s) 867, 979, 1968
BstNSI RCATGY 3 cut(s) 200, 239, 942
BstSCI CCNGG 3 cut(s) 865, 977, 1966
BstSFI CTRYAG 1 cut(s) 795
BstSLI GKGCMC 2 cut(s) 2614, 3203
BstUI CGCG 1 cut(s) 615
BstV1I GCAGC 7 cut(s) 439, 781, 986, 1746, 1909, 2051, 3003
BstV2I GAAGAC 2 cut(s) 14, 884
BstX2I RGATCY 1 cut(s) 379
BstXI CCANNNNNNTGG 2 cut(s) 2783, 3219
BstYI RGATCY 1 cut(s) 379
Bsu15I ATCGAT 1 cut(s) 531
Bsu36I CCTNAGG 1 cut(s) 1767
BsuI GTATCC 2 cut(s) 2081, 3154
BsuRI GGCC 3 cut(s) 737, 754, 3223
BsuTUI ATCGAT 1 cut(s) 531
BtgZI GCGATG 1 cut(s) 99
BtsI GCAGTG 1 cut(s) 3201
Cac8I GCNNGC 3 cut(s) 237, 1823, 1834
CaiI CAGNNNCTG 3 cut(s) 872, 2045, 3329
CciI TCATGA 2 cut(s) 591, 2968
CfoI GCGC 2 cut(s) 1141, 2217
Cfr10I RCCGGY 1 cut(s) 3223
Cfr13I GGNCC 4 cut(s) 736, 753, 860, 2183
ClaI ATCGAT 1 cut(s) 531
CseI GACGC 5 cut(s) 2471, 2631, 2709, 2924, 3116
CspCI CAANNNNNGTGG 2 cut(s) 1884, 1919
DdeI CTNAG 9 cut(s) 80, 132, 983, 1659, 1767, 1926, 2046, 2331, 2952
DinI GGCGCC 1 cut(s) 1140
DraI TTTAAA 3 cut(s) 804, 1630, 1671
DriI GACNNNNNGTC 1 cut(s) 891
EaeI YGGCCR 1 cut(s) 3221
Eam1104I CTCTTC 5 cut(s) 1197, 1328, 1382, 1883, 2508
Eam1105I GACNNNNNGTC 1 cut(s) 891
EarI CTCTTC 5 cut(s) 1197, 1328, 1382, 1883, 2508
EciI GGCGGA 1 cut(s) 1964
Eco130I CCWWGG 4 cut(s) 122, 2503, 2649, 2694
Eco31I GGTCTC 6 cut(s) 81, 128, 1885, 1976, 2332, 3430
Eco47I GGWCC 2 cut(s) 860, 2183
Eco57I CTGAAG 4 cut(s) 1547, 1601, 1775, 3087
Eco72I CACGTG 1 cut(s) 2609
Eco81I CCTNAGG 1 cut(s) 1767
EcoNI CCTNNNNNAGG 1 cut(s) 1091
EcoO109I RGGNCCY 1 cut(s) 860
EcoRI GAATTC 3 cut(s) 1772, 2357, 3055
EcoRII CCWGG 3 cut(s) 865, 977, 1966
EcoT14I CCWWGG 4 cut(s) 122, 2503, 2649, 2694
EcoT22I ATGCAT 2 cut(s) 1644, 2127
EgeI GGCGCC 1 cut(s) 1140
EheI GGCGCC 1 cut(s) 1140
ErhI CCWWGG 4 cut(s) 122, 2503, 2649, 2694
Esp3I CGTCTC 1 cut(s) 2990
FalI AAGNNNNNCTT 2 cut(s) 2822, 2854
FaqI GGGAC 4 cut(s) 1766, 2712, 2989, 3271
FauNDI CATATG 1 cut(s) 685
FbaI TGATCA 5 cut(s) 219, 1105, 1125, 2284, 2845
FblI GTMKAC 4 cut(s) 1183, 2645, 2788, 3359
FspBI CTAG 5 cut(s) 123, 2681, 3114, 3119, 3234
GlaI GCGC 2 cut(s) 1140, 2216
GsuI CTGGAG 2 cut(s) 849, 2786
HaeII RGCGCY 2 cut(s) 1142, 2218
HaeIII GGCC 3 cut(s) 737, 754, 3223
HapII CCGG 3 cut(s) 479, 734, 3224
HgaI GACGC 5 cut(s) 2471, 2631, 2709, 2924, 3116
HhaI GCGC 2 cut(s) 1141, 2217
Hin1I GRCGYC 3 cut(s) 1139, 2935, 3135
Hin6I GCGC 2 cut(s) 1139, 2215
HinP1I GCGC 2 cut(s) 1139, 2215
HincII GTYRAC 2 cut(s) 2646, 2656
HindII GTYRAC 2 cut(s) 2646, 2656
HindIII AAGCTT 2 cut(s) 1605, 2918
HpaI GTTAAC 1 cut(s) 2656
HpaII CCGG 3 cut(s) 479, 734, 3224
HphI GGTGA 6 cut(s) 1600, 2391, 2449, 2522, 3085, 3402
Hpy166II GTNNAC 8 cut(s) 507, 1184, 1751, 2612, 2646, 2656, 2789, 3360
Hpy8I GTNNAC 8 cut(s) 507, 1184, 1751, 2612, 2646, 2656, 2789, 3360
Hpy99I CGWCG 1 cut(s) 2647
HpyCH4IV ACGT 7 cut(s) 15, 1180, 1478, 2608, 2999, 3135, 3417
HpyF10VI GCNNNNNNNGC 3 cut(s) 2042, 2597, 2948
HpyF3I CTNAG 9 cut(s) 80, 132, 983, 1659, 1767, 1926, 2046, 2331, 2952
HpySE526I ACGT 7 cut(s) 15, 1180, 1478, 2608, 2999, 3135, 3417
Hsp92I GRCGYC 3 cut(s) 1139, 2935, 3135
HspAI GCGC 2 cut(s) 1139, 2215
KasI GGCGCC 1 cut(s) 1138
Kpn2I TCCGGA 1 cut(s) 478
Ksp22I TGATCA 5 cut(s) 219, 1105, 1125, 2284, 2845
KspAI GTTAAC 1 cut(s) 2656
LmnI GCTCC 2 cut(s) 949, 2767
Lsp1109I GCAGC 7 cut(s) 439, 781, 986, 1746, 1909, 2051, 3003
LweI GCATC 8 cut(s) 484, 1168, 1862, 1889, 2107, 2112, 2134, 2680
MaeI CTAG 5 cut(s) 123, 2681, 3114, 3119, 3234
MaeII ACGT 7 cut(s) 15, 1180, 1478, 2608, 2999, 3135, 3417
MaeIII GTNAC 8 cut(s) 34, 148, 1002, 2191, 2459, 2660, 3094, 3239
MbiI CCGCTC 1 cut(s) 2002
MfeI CAATTG 3 cut(s) 2562, 2704, 2881
MflI RGATCY 1 cut(s) 379
MhlI GDGCHC 8 cut(s) 731, 946, 1255, 1309, 1363, 1417, 2614, 3203
Mly113I GGCGCC 1 cut(s) 1139
MlyI GAGTC 6 cut(s) 253, 776, 1952, 2400, 2657, 3438
MmeI TCCRAC 5 cut(s) 355, 2279, 2292, 2601, 3406
Mph1103I ATGCAT 2 cut(s) 1644, 2127
MroI TCCGGA 1 cut(s) 478
MroXI GAANNNNTTC 2 cut(s) 431, 486
MslI CAYNNNNRTG 4 cut(s) 596, 837, 937, 3071
MspA1I CMGCKG 1 cut(s) 2042
MspI CCGG 3 cut(s) 479, 734, 3224
MspR9I CCNGG 3 cut(s) 867, 979, 1968
MunI CAATTG 3 cut(s) 2562, 2704, 2881
MvaI CCWGG 3 cut(s) 867, 979, 1968
MvnI CGCG 1 cut(s) 615
MwoI GCNNNNNNNGC 3 cut(s) 2042, 2597, 2948
NarI GGCGCC 1 cut(s) 1139
NdeI CATATG 1 cut(s) 685
NlaIV GGNNCC 6 cut(s) 120, 434, 861, 1140, 1194, 2501
NmuCI GTSAC 6 cut(s) 148, 1002, 2191, 2459, 2660, 3094
NsiI ATGCAT 2 cut(s) 1644, 2127
NspI RCATGY 3 cut(s) 200, 239, 942
NspV TTCGAA 1 cut(s) 2067
PaeI GCATGC 1 cut(s) 239
PagI TCATGA 2 cut(s) 591, 2968
PciI ACATGT 1 cut(s) 938
PdmI GAANNNNTTC 2 cut(s) 431, 486
PfeI GAWTC 7 cut(s) 127, 1508, 2469, 2801, 3022, 3169, 3230
PflMI CCANNNNNTGG 3 cut(s) 298, 374, 872
PleI GAGTC 6 cut(s) 253, 776, 1951, 2399, 2657, 3437
PluTI GGCGCC 1 cut(s) 1142
PmaCI CACGTG 1 cut(s) 2609
PmlI CACGTG 1 cut(s) 2609
PpsI GAGTC 6 cut(s) 253, 776, 1951, 2399, 2657, 3437
Ppu21I YACGTR 1 cut(s) 2609
PpuMI RGGWCCY 1 cut(s) 860
PscI ACATGT 1 cut(s) 938
PshBI ATTAAT 2 cut(s) 356, 851
PsiI TTATAA 2 cut(s) 2024, 2163
Psp5II RGGWCCY 1 cut(s) 860
Psp6I CCWGG 3 cut(s) 865, 977, 1966
PspCI CACGTG 1 cut(s) 2609
PspGI CCWGG 3 cut(s) 865, 977, 1966
PspN4I GGNNCC 6 cut(s) 120, 434, 861, 1140, 1194, 2501
PspPI GGNCC 4 cut(s) 736, 753, 860, 2183
PspPPI RGGWCCY 1 cut(s) 860
PstI CTGCAG 1 cut(s) 799
PstNI CAGNNNCTG 3 cut(s) 872, 2045, 3329
PsuI RGATCY 1 cut(s) 379
RseI CAYNNNNRTG 4 cut(s) 596, 837, 937, 3071
SalI GTCGAC 1 cut(s) 2644
Sau96I GGNCC 4 cut(s) 736, 753, 860, 2183
ScaI AGTACT 2 cut(s) 1208, 2685
SchI GAGTC 6 cut(s) 253, 776, 1952, 2400, 2657, 3438
ScrFI CCNGG 3 cut(s) 867, 979, 1968
SduI GDGCHC 8 cut(s) 731, 946, 1255, 1309, 1363, 1417, 2614, 3203
SfaNI GCATC 8 cut(s) 484, 1168, 1862, 1889, 2107, 2112, 2134, 2680
SfcI CTRYAG 1 cut(s) 795
SfoI GGCGCC 1 cut(s) 1140
SfuI TTCGAA 1 cut(s) 2067
SinI GGWCC 2 cut(s) 860, 2183
SmiMI CAYNNNNRTG 4 cut(s) 596, 837, 937, 3071
SphI GCATGC 1 cut(s) 239
SsiI CCGC 9 cut(s) 335, 959, 1947, 1975, 2002, 2036, 2042, 2485, 3278
SspDI GGCGCC 1 cut(s) 1138
SspI AATATT 6 cut(s) 255, 610, 655, 955, 1842, 2797
SspMI CTAG 5 cut(s) 123, 2681, 3114, 3119, 3234
StyD4I CCNGG 3 cut(s) 865, 977, 1966
StyI CCWWGG 4 cut(s) 122, 2503, 2649, 2694
TaiI ACGT 7 cut(s) 18, 1183, 1481, 2611, 3002, 3138, 3420
TaqI TCGA 6 cut(s) 57, 186, 531, 2067, 2467, 2645
TaqII GACCGA 2 cut(s) 990, 3281
TatI WGTACW 4 cut(s) 701, 1206, 1847, 2683
TauI GCSGC 4 cut(s) 2005, 2039, 2045, 3280
TfiI GAWTC 7 cut(s) 127, 1508, 2469, 2801, 3022, 3169, 3230
TseFI GTSAC 6 cut(s) 148, 1002, 2191, 2459, 2660, 3094
TseI GCWGC 7 cut(s) 452, 794, 974, 1759, 1922, 2039, 3016
Tsp45I GTSAC 6 cut(s) 148, 1002, 2191, 2459, 2660, 3094
TspGWI ACGGA 5 cut(s) 239, 410, 564, 1038, 2464
Van91I CCANNNNNTGG 3 cut(s) 298, 374, 872
VneI GTGCAC 1 cut(s) 2610
VpaK11BI GGWCC 2 cut(s) 860, 2183
VspI ATTAAT 2 cut(s) 356, 851
XagI CCTNNNNNAGG 1 cut(s) 1091
XapI RAATTY 9 cut(s) 295, 485, 1502, 1543, 1672, 1772, 2279, 2357, 3055
XceI RCATGY 3 cut(s) 200, 239, 942
XcmI CCANNNNNNNNNTGG 1 cut(s) 2761
XmaJI CCTAGG 1 cut(s) 122
XmiI GTMKAC 4 cut(s) 1183, 2645, 2788, 3359
XmnI GAANNNNTTC 2 cut(s) 431, 486
XspI CTAG 5 cut(s) 123, 2681, 3114, 3119, 3234
ZraI GACGTC 1 cut(s) 3136
ZrmI AGTACT 2 cut(s) 1208, 2685
Zsp2I ATGCAT 2 cut(s) 1644, 2127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.