MD08G1078300.v1.1

AAA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000279
Physical Location & Seq
Forward (+)
6510393 .. 6515259
4867 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1078300.v1.1.491

Sequence Viewer

Length: 2724 bp
ATGAATCGTCAGGAGGAGAGCAATAAGTTGAACAATTATGGAGTTGCAGCAGGTCGAGGCTTAATCAATGTCGTATTCTCTTGGTCACCTAAGGATGTTCTCAATGCAAAGCTTTACGAAAATCAGGTGACAAAGATTCCTGAGACATTTTCTACCACGACGAGTTACATGAAATCTTTCATCCCTTCACTTGTTGAGGAAACACATGCTGATCTACTATCAAGCATGACGAATCTATCGAAGGCACCTACTTGCGAACTTCGGACTGTTACAGCTTCTGAGAATCATAGACCTCCTAAGGACTTGTTTTATGATGTTACTTGTGTAGGAACGTATGTGCCACAGGTTGGAGATCTCATTGCCTTGACTGACATTAAACCAAAATGCGTTGATCATTTGTACAGGTCCAGAAATTCGTATCTCATTGCGTATGTTCATCAAATTAGAGACAATAATCTCTCAGTACTCTCGTCCAAGCATATCGATACAAGAGGATACACGCATGCTGCGGGCAAGAGAGAAACAATGTTTGCTGTCTATTTGATGAACATGACAACAAATATTCGTGCATGGAAAGCCTTGAATTCAGATGACGCAAACACAAATCTAATTAAGACTGTGTTGCAACTGCAACCCAGTTCATCACATGGTGGGAGTTCATGTACAATTTGCTTTTCAAAAGAAAAGTGCTTCGATTCCCTTTCGACTAAATGGCCAGAAATGTTGTGTGATCTAAATGAATCCCAAAAAGCTGCAGTTTTAAACTCTATCAGTTTGAGTAGATGCAAACACCAAAATTCCATCAATCTAATATGGGGCCCTCCTGGGACTGGGAAAACGAAGACAGTCGGCATCTCACTCTTTGCCCTCTATAAGTTCAAGTGCAGAACACTAACATGTGCCCCAACCAATGTCGCTGTGTTAGAAGTTACAGATCGACTCGTGAGGTTGGTTAACCAGTCTCTTGAGTATGATAAGTACGGGCTTGGGGACATAATTCTATTTGGGAATGGTGAGCGAATGAAGATTGGTAGTTATAATTATCTTTTTGAGGTATTTCTTGATAGGCGTATTAGTATTCTGACCAAGTGTTTTTCCCCGTTGAGTGGATGGAATCATTGGTTAGAATTGATGATAAGTTTGCTTGAGAATCCAGAGGAGCAGTACTCATTGTACATAAGGGAAAAACAGAAGCATGACGAGCAACATAAAGAAAGTGAAAACAGCAACTCATCAAGCGATGATGAGAACAACTTTCTGACATTTGAGAAGTCTGTGAAGGAAAAACACATTAAGGATGACAAGCAAGATGAAGAGAGTGAAAATAGCAGCTCATCAAGTGATGATAAGAATGTTATTCTGGCGTTCGAGGAGTTTGTGAATAACCGTCTTGATTTCATTGGTCAGCATTTGACAGTTTGTATGGTAAATTTGTACACTCACTTGCCAACATCTTGCATTTCACTTGAGGTGGTGAAGGACATGATCAGAGTTGCAGGGTTGCTTAAGTCGATTAAATCTATATTTAGGTGGGCTGGCGTTACTAATAAGCAGTTAAAATTACTTCAAGAAGATTGCACTCGCACTCTCAAGTCACTTCGTGCGTTTGCAGTTCCGAGTTCAAATGATGAACAAACAATAAGAAATTTGTGCCTGGCAAAGGCTTGCCTAATATTTTGTACCGCATCAAGTTCTGCCAGACTGCATACAGATGGAATGGTGCCCCTGGAATTGTTAGTCATTGATGAAGCTGCTCAGCTTAAAGAATGTGAGTCAACAATTCCATTACAACTACCAGGTCTTCGGCATGCTATTCTCATAGGAGATGAGAGGCAACTCCCTGCAGTGGTTAAAAGCCAGATCTCCGAGAAGGCTGAATTTGGAAGAAGTTTGTTTGAAAGACTTGTGCAGTTGGGACACAAGAAGCACCTTCTCAATGTCCAGTATAGGATGCATCCTTCAATCAGCTTGTTTCCGAAAATGGAGTTTTACGACAATCAGATATTAGATGGTCCAAATGTCAGTGAAGTGAGCTACAACAAGTGCTTCCTTGATGGAAAAATGTACGGATCCTACTCATTTATAAACATAGCAAATGGGAAAGAAGAATTTGATGGCGGGCGTAGTCCGAAAAACGTGGCTGAGGTTGCTGTGGTTTATGAGATAGTTTCTAGGCTTTACAAAGAATTCACTCGCACAAAAAAGAAGGTTAGTGTTGGGGTAATATCACCTTACACGGCTCAAGTTAATGCAATTCAAGAGAGAGTCAAAGAGTATAGTGAAGTTTCGGCTGGCTCAGACTTCTCCATGAGCGTGCGATCTGTTGATGGATTCCAAGGTGGTGAAGAGGATTTGATAATTATATCCACTGTCAGATGTAATGGAAAGGGCTCTGTGGGATTCCTCTCCAATCGTCAACGAGCAAATGTGGTTCTAACACGCGCAAGGCATTGTCTTTGGATACTGGGGCACGAATTGACCTTGATTAACAGCAACTCAATTTGGAAGAAGCTAATTCTTGACGCGAAGAAACGAGAATGTTTTTTTAATGCTGATGAGGATGAGAAGTTGGCTCAGGCTATGGCCGCAGCCCAATCGAGTTGCGATGAAGATGATCCAATGGAGCTCCTTGCAAGACCACTTTCTTCACTCAGACTGACTGATCGGACAGCTGAAACATCAACTCCAACTTACAGGTGTGAACAATTTTCGGCGCGGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12

Protein Analysis

908

Amino Acids

102.19

Weight (kDa)

7.04

Isoelectric Point (pI)

43.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 114 - 195 2.9e-09 Domain of unknown function (DUF6469)
AAA_11 PF13086 245 - 620 1.7e-33 AAA domain
AAA_12 PF13087 628 - 825 1.2e-54 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000279)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65810 AT1G65810 AT1G65810 AT4G05540 AT5G37140 AT5G37150 AT5G37160 AT5G37165 AT5G52090
fragaria_vesca FvH4_1g27541 FvH4_1g27542 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_3g00170 FvH4_3g00170 FvH4_3g00170 FvH4_5g05930 FvH4_5g05930
malus_domestica MD08G1077700.v1.1 MD08G1077900.v1.1 MD08G1078000.v1.1 MD08G1078100.v1.1 MD08G1078300.v1.1 MD09G1057800.v1.1 MD09G1281400.v1.1 MD11G1222900.v1.1 MD14G1146400.v1.1
prunus_persica Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G202100_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.3G013100_v2.0.a1
pyrus_communis pycom09g18900 pycom09g18910 pycom13g26720 pycom13g26730 pycom13g26760 pycom13g26770
rosa_chinensis RchiOBHm_Chr3g0488001 RchiOBHm_Chr3g0488011 RchiOBHm_Chr3g0488031 RchiOBHm_Chr3g0488061 RchiOBHm_Chr3g0488111 RchiOBHm_Chr3g0488201 RchiOBHm_Chr3g0488211 RchiOBHm_Chr3g0488221 RchiOBHm_Chr3g0488321 RchiOBHm_Chr3g0488431 RchiOBHm_Chr6g0264601 RchiOBHm_Chr6g0264641
rosa_laevigata RLG00000014240 RLG00000014241 RLG00000022910 RLG00000022949 RLG00000030376
rosa_multiflora Rmu_co8075388.1_g000001 Rmu_co8424869.1_g000001 Rmu_co8427783.1_g000001 Rmu_sc0000546.1_g000002 Rmu_sc0000546.1_g000015 Rmu_sc0011052.1_g000014 Rmu_sc0017772.1_g000004
rosa_roxburghii Rroxscaffold_175G00431980 Rroxscaffold_175G00431990 Rroxscaffold_1G00034600 Rroxscaffold_6G00394230 Rroxscaffold_6G00394270 Rroxscaffold_6G00394280 Rroxscaffold_6G00394350 Rroxscaffold_7G00203240 Rroxscaffold_7G00203270 Rroxscaffold_7G00203280 Rroxscaffold_7G00203300 Rroxscaffold_7G00205740
rosa_rugosa Rorug03G0238000 Rorug03G0238100 Rorug03G0238100 Rorug03G0238500 Rorug05G0155400 Rorug05G0356000 Rorug05G0463000 Rorug06G0015200 Rorug06G0015300 Rorug06G0015400 Rorug06G0015500 Rorug06G0015500
rosa_samantha Rh1DG088100 Rh2AG244800 Rh2BG187500 Rh2CG247300 Rh3BG322700 Rh3BG322800 Rh3BG323100 Rh3BG323900 Rh3BG324000 Rh3BG324300 Rh3CG321300 Rh3CG321400 Rh3CG321500 Rh3DG319400 Rh3DG319900 Rh3DG320100 Rh3DG320200 Rh6AG133700 Rh6AG133800 Rh6AG133900 Rh6CG129300 Rh6CG129800 Rh6CG130200 Rh6DG116500 Rh6DG117200 Rh7DG378000
rosa_wichuraiana Rw2G014710 Rw3G025340 Rw3G025350 Rw3G025360 Rw5G020630 Rw6G011590 Rw6G011600 Rw7G041380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1038, 2084
Acc36I ACCTGC 1 cut(s) 41
AccB1I GGYRCC 2 cut(s) 244, 1720
AccB7I CCANNNNNTGG 1 cut(s) 347
AccII CGCG 3 cut(s) 2442, 2525, 2716
AciI CCGC 5 cut(s) 509, 1683, 2118, 2586, 2716
AclWI GGATC 3 cut(s) 2064, 2077, 2609
AcoI YGGCCR 2 cut(s) 713, 2583
AcsI RAATTY 8 cut(s) 412, 583, 796, 1429, 1645, 1877, 2108, 2186
AdeI CACNNNGTG 2 cut(s) 650, 1601
AfaI GTAC 9 cut(s) 401, 465, 664, 980, 1166, 1175, 1436, 1681, 2066
AfiI CCNNNNNNNGG 5 cut(s) 347, 830, 1106, 1660, 1846
AflII CTTAAG 1 cut(s) 1505
AflIII ACRYGT 1 cut(s) 896
AgsI TTSAA 9 cut(s) 31, 583, 678, 880, 1568, 1623, 1898, 1962, 2258
AjnI CCWGG 4 cut(s) 823, 1653, 1725, 1795
Alw21I GWGCWC 1 cut(s) 2628
Alw26I GTCTC 3 cut(s) 137, 441, 966
AlwI GGATC 3 cut(s) 2064, 2077, 2609
AlwNI CAGNNNCTG 1 cut(s) 278
AoxI GGCC 3 cut(s) 713, 817, 2583
ApaI GGGCCC 1 cut(s) 821
ApeKI GCWGC 6 cut(s) 47, 506, 752, 1329, 1751, 2588
ApoI RAATTY 8 cut(s) 412, 583, 796, 1429, 1645, 1877, 2108, 2186
Asp700I GAANNNNTTC 1 cut(s) 176
AspLEI GCGC 2 cut(s) 2444, 2716
AspS9I GGNCC 4 cut(s) 405, 817, 818, 2012
AsuHPI GGTGA 6 cut(s) 78, 139, 1025, 1486, 2220, 2354
AvaII GGWCC 2 cut(s) 405, 2012
AxyI CCTNAGG 2 cut(s) 90, 297
BaeGI GKGCMC 4 cut(s) 821, 904, 1725, 2472
BalI TGGCCA 1 cut(s) 715
BamHI GGATCC 1 cut(s) 2069
BanI GGYRCC 2 cut(s) 244, 1720
BanII GRGCYC 3 cut(s) 821, 2393, 2628
BauI CACGAG 1 cut(s) 941
BbsI GAAGAC 2 cut(s) 848, 1793
Bbv12I GWGCWC 1 cut(s) 2628
BbvCI CCTCAGC 1 cut(s) 2142
BbvI GCAGC 6 cut(s) 59, 493, 739, 1341, 1738, 2600
BccI CCATC 7 cut(s) 809, 1104, 1706, 2003, 2048, 2108, 2321
BceAI ACGGC 1 cut(s) 2253
BcgI CGANNNNNNTGC 2 cut(s) 2577, 2611
BciT130I CCWGG 4 cut(s) 825, 1655, 1727, 1797
BciVI GTATCC 2 cut(s) 488, 2454
BclI TGATCA 2 cut(s) 391, 1485
BcoDI GTCTC 3 cut(s) 137, 441, 966
BfaI CTAG 2 cut(s) 2172, 2722
BfmI CTRYAG 2 cut(s) 753, 1842
BfrI CTTAAG 1 cut(s) 1505
BfuAI ACCTGC 1 cut(s) 41
BfuI GTATCC 2 cut(s) 488, 2454
BglII AGATCT 2 cut(s) 352, 1860
BisI GCNGC 7 cut(s) 48, 507, 753, 1330, 1752, 2586, 2589
BlpI GCTNAGC 1 cut(s) 1755
BlsI GCNGC 7 cut(s) 49, 508, 754, 1331, 1753, 2587, 2590
BmcAI AGTACT 2 cut(s) 465, 1166
Bme1390I CCNGG 4 cut(s) 825, 1655, 1727, 1797
Bme18I GGWCC 2 cut(s) 405, 2012
BmgT120I GGNCC 4 cut(s) 405, 817, 818, 2012
BmiI GGNNCC 5 cut(s) 246, 818, 819, 1722, 2071
BmrFI CCNGG 4 cut(s) 825, 1655, 1727, 1797
BmrI ACTGGG 3 cut(s) 630, 840, 2474
BmsI GCATC 5 cut(s) 773, 861, 1694, 1941, 1963
BmuI ACTGGG 3 cut(s) 630, 840, 2474
BpiI GAAGAC 2 cut(s) 848, 1793
BplI GAGNNNNNCTC 2 cut(s) 1151, 1183
Bpu10I CCTNAGC 2 cut(s) 2142, 2574
Bpu1102I GCTNAGC 1 cut(s) 1755
BpuEI CTTGAG 5 cut(s) 986, 1166, 1487, 1574, 2226
Bsa29I ATCGAT 1 cut(s) 483
BsaJI CCNNGG 3 cut(s) 824, 1725, 2335
BsaXI ACNNNNNCTCC 4 cut(s) 646, 676, 2668, 2698
Bsc4I CCNNNNNNNGG 5 cut(s) 347, 830, 1106, 1660, 1846
Bse1I ACTGG 5 cut(s) 636, 835, 958, 1942, 2469
Bse21I CCTNAGG 2 cut(s) 90, 297
Bse3DI GCAATG 2 cut(s) 357, 423
BseBI CCWGG 4 cut(s) 825, 1655, 1727, 1797
BseCI ATCGAT 1 cut(s) 483
BseDI CCNNGG 3 cut(s) 824, 1725, 2335
BseGI GGATG 7 cut(s) 100, 180, 1115, 1303, 1954, 1956, 2566
BseLI CCNNNNNNNGG 5 cut(s) 347, 830, 1106, 1660, 1846
BseMI GCAATG 2 cut(s) 357, 423
BseMII CTCAG 8 cut(s) 132, 270, 474, 1769, 2133, 2310, 2588, 2665
BseNI ACTGG 5 cut(s) 636, 835, 958, 1942, 2469
BseRI GAGGAG 3 cut(s) 29, 1172, 1385
BseSI GKGCMC 4 cut(s) 821, 904, 1725, 2472
BseXI GCAGC 6 cut(s) 59, 493, 739, 1341, 1738, 2600
BsgI GTGCAG 2 cut(s) 904, 1928
Bsh1236I CGCG 3 cut(s) 2442, 2525, 2716
BshFI GGCC 3 cut(s) 715, 819, 2585
BshNI GGYRCC 2 cut(s) 244, 1720
BshVI ATCGAT 1 cut(s) 483
BsiHKAI GWGCWC 1 cut(s) 2628
BslFI GGGAC 3 cut(s) 841, 1004, 1929
BslI CCNNNNNNNGG 5 cut(s) 347, 830, 1106, 1660, 1846
BsmAI GTCTC 3 cut(s) 137, 441, 966
BsmFI GGGAC 3 cut(s) 841, 1004, 1929
BsnI GGCC 3 cut(s) 715, 819, 2585
Bsp120I GGGCCC 1 cut(s) 817
Bsp1286I GDGCHC 6 cut(s) 821, 904, 1725, 2393, 2472, 2628
Bsp1407I TGTACA 4 cut(s) 399, 662, 1173, 1434
Bsp1720I GCTNAGC 1 cut(s) 1755
BspACI CCGC 5 cut(s) 509, 1683, 2118, 2586, 2716
BspANI GGCC 3 cut(s) 715, 819, 2585
BspCNI CTCAG 8 cut(s) 133, 271, 473, 1768, 2134, 2309, 2587, 2664
BspDI ATCGAT 1 cut(s) 483
BspFNI CGCG 3 cut(s) 2442, 2525, 2716
BspLI GGNNCC 5 cut(s) 246, 818, 819, 1722, 2071
BspMAI CTGCAG 2 cut(s) 757, 1846
BspMI ACCTGC 1 cut(s) 41
BspPI GGATC 3 cut(s) 2064, 2077, 2609
BspT107I GGYRCC 2 cut(s) 244, 1720
BspTI CTTAAG 1 cut(s) 1505
BsrDI GCAATG 2 cut(s) 357, 423
BsrGI TGTACA 4 cut(s) 399, 662, 1173, 1434
BsrI ACTGG 5 cut(s) 636, 835, 958, 1942, 2469
BssECI CCNNGG 3 cut(s) 824, 1725, 2335
BssSI CACGAG 1 cut(s) 941
BssT1I CCWWGG 1 cut(s) 2335
Bst2BI CACGAG 1 cut(s) 941
Bst2UI CCWGG 4 cut(s) 825, 1655, 1727, 1797
Bst4CI ACNGT 6 cut(s) 268, 619, 847, 1388, 1417, 2371
Bst6I CTCTTC 2 cut(s) 1308, 2340
BstAFI CTTAAG 1 cut(s) 1505
BstAUI TGTACA 4 cut(s) 399, 662, 1173, 1434
BstC8I GCNNGC 8 cut(s) 504, 511, 1537, 1666, 1809, 2120, 2293, 2315
BstEII GGTNACC 1 cut(s) 84
BstENI CCTNNNNNAGG 1 cut(s) 1658
BstF5I GGATG 7 cut(s) 100, 180, 1115, 1303, 1954, 1956, 2566
BstFNI CGCG 3 cut(s) 2442, 2525, 2716
BstHHI GCGC 2 cut(s) 2444, 2716
BstMAI GTCTC 3 cut(s) 137, 441, 966
BstMWI GCNNNNNNNGC 4 cut(s) 575, 1201, 2147, 2585
BstNI CCWGG 4 cut(s) 825, 1655, 1727, 1797
BstNSI RCATGY 4 cut(s) 209, 506, 900, 1811
BstPI GGTNACC 1 cut(s) 84
BstSCI CCNGG 4 cut(s) 823, 1653, 1725, 1795
BstSFI CTRYAG 2 cut(s) 753, 1842
BstSLI GKGCMC 4 cut(s) 821, 904, 1725, 2472
BstUI CGCG 3 cut(s) 2442, 2525, 2716
BstV1I GCAGC 6 cut(s) 59, 493, 739, 1341, 1738, 2600
BstV2I GAAGAC 2 cut(s) 848, 1793
BstX2I RGATCY 3 cut(s) 352, 1860, 2069
BstYI RGATCY 3 cut(s) 352, 1860, 2069
Bsu15I ATCGAT 1 cut(s) 483
Bsu36I CCTNAGG 2 cut(s) 90, 297
BsuI GTATCC 2 cut(s) 488, 2454
BsuRI GGCC 3 cut(s) 715, 819, 2585
BsuTUI ATCGAT 1 cut(s) 483
BtgZI GCGATG 2 cut(s) 1254, 2619
BtsCI GGATG 7 cut(s) 100, 180, 1115, 1303, 1954, 1956, 2566
BtsI GCAGTG 1 cut(s) 1851
BtsIMutI CAGTG 3 cut(s) 1851, 2029, 2367
BveI ACCTGC 1 cut(s) 41
Cac8I GCNNGC 8 cut(s) 504, 511, 1537, 1666, 1809, 2120, 2293, 2315
CaiI CAGNNNCTG 1 cut(s) 278
CfoI GCGC 2 cut(s) 2444, 2716
Cfr13I GGNCC 4 cut(s) 405, 817, 818, 2012
ClaI ATCGAT 1 cut(s) 483
CseI GACGC 2 cut(s) 602, 2531
CsiI ACCWGGT 1 cut(s) 1795
Csp6I GTAC 9 cut(s) 400, 464, 663, 979, 1165, 1174, 1435, 1680, 2065
CviQI GTAC 9 cut(s) 400, 464, 663, 979, 1165, 1174, 1435, 1680, 2065
DraI TTTAAA 1 cut(s) 762
DraIII CACNNNGTG 2 cut(s) 650, 1601
EaeI YGGCCR 2 cut(s) 713, 2583
Eam1104I CTCTTC 2 cut(s) 1308, 2340
EarI CTCTTC 2 cut(s) 1308, 2340
Ecl136II GAGCTC 1 cut(s) 2626
Eco130I CCWWGG 1 cut(s) 2335
Eco24I GRGCYC 3 cut(s) 821, 2393, 2628
Eco47I GGWCC 2 cut(s) 405, 2012
Eco53kI GAGCTC 1 cut(s) 2626
Eco81I CCTNAGG 2 cut(s) 90, 297
Eco91I GGTNACC 1 cut(s) 84
EcoICRI GAGCTC 1 cut(s) 2626
EcoNI CCTNNNNNAGG 1 cut(s) 1658
EcoO109I RGGNCCY 2 cut(s) 817, 818
EcoO65I GGTNACC 1 cut(s) 84
EcoRI GAATTC 2 cut(s) 583, 2186
EcoRII CCWGG 4 cut(s) 823, 1653, 1725, 1795
EcoT14I CCWWGG 1 cut(s) 2335
EcoT22I ATGCAT 1 cut(s) 1956
EcoT38I GRGCYC 3 cut(s) 821, 2393, 2628
ErhI CCWWGG 1 cut(s) 2335
FaqI GGGAC 3 cut(s) 841, 1004, 1929
FauI CCCGC 2 cut(s) 502, 2111
FbaI TGATCA 2 cut(s) 391, 1485
Fnu4HI GCNGC 7 cut(s) 48, 507, 753, 1330, 1752, 2586, 2589
FokI GGATG 7 cut(s) 107, 167, 1122, 1310, 1941, 1963, 2573
FriOI GRGCYC 3 cut(s) 821, 2393, 2628
Fsp4HI GCNGC 7 cut(s) 48, 507, 753, 1330, 1752, 2586, 2589
FspBI CTAG 2 cut(s) 2172, 2722
GlaI GCGC 2 cut(s) 2443, 2715
GluI GCNGC 7 cut(s) 48, 507, 753, 1330, 1752, 2586, 2589
HaeIII GGCC 3 cut(s) 715, 819, 2585
HgaI GACGC 2 cut(s) 602, 2531
HhaI GCGC 2 cut(s) 2444, 2716
Hin6I GCGC 2 cut(s) 2442, 2714
HinP1I GCGC 2 cut(s) 2442, 2714
HincII GTYRAC 3 cut(s) 955, 1776, 2417
HindII GTYRAC 3 cut(s) 955, 1776, 2417
HindIII AAGCTT 1 cut(s) 110
HpaI GTTAAC 1 cut(s) 955
HphI GGTGA 6 cut(s) 78, 139, 1025, 1486, 2220, 2354
Hpy166II GTNNAC 5 cut(s) 955, 1437, 1776, 2417, 2702
Hpy8I GTNNAC 5 cut(s) 955, 1437, 1776, 2417, 2702
Hpy99I CGWCG 1 cut(s) 163
HpyAV CCTTC 8 cut(s) 195, 235, 1273, 1471, 1864, 1940, 1968, 2200
HpyCH4III ACNGT 6 cut(s) 268, 619, 847, 1388, 1417, 2371
HpyCH4IV ACGT 2 cut(s) 332, 2136
HpyF10VI GCNNNNNNNGC 4 cut(s) 575, 1201, 2147, 2585
HpySE526I ACGT 2 cut(s) 332, 2136
HspAI GCGC 2 cut(s) 2442, 2714
Ksp22I TGATCA 2 cut(s) 391, 1485
KspAI GTTAAC 1 cut(s) 955
LmnI GCTCC 4 cut(s) 1159, 2623, 2631, 2718
Lsp1109I GCAGC 6 cut(s) 59, 493, 739, 1341, 1738, 2600
LweI GCATC 5 cut(s) 773, 861, 1694, 1941, 1963
MabI ACCWGGT 1 cut(s) 1795
MaeI CTAG 2 cut(s) 2172, 2722
MaeII ACGT 2 cut(s) 332, 2136
MaeIII GTNAC 8 cut(s) 84, 127, 164, 268, 316, 928, 1540, 1593
MflI RGATCY 3 cut(s) 352, 1860, 2069
MhlI GDGCHC 6 cut(s) 821, 904, 1725, 2393, 2472, 2628
MlsI TGGCCA 1 cut(s) 715
MluNI TGGCCA 1 cut(s) 715
MlyI GAGTC 3 cut(s) 933, 1781, 2274
MmeI TCCRAC 2 cut(s) 328, 2711
Mox20I TGGCCA 1 cut(s) 715
Mph1103I ATGCAT 1 cut(s) 1956
MroXI GAANNNNTTC 1 cut(s) 176
MscI TGGCCA 1 cut(s) 715
MslI CAYNNNNRTG 3 cut(s) 895, 1710, 2312
Msp20I TGGCCA 1 cut(s) 715
MspA1I CMGCKG 1 cut(s) 2672
MspCI CTTAAG 1 cut(s) 1505
MspR9I CCNGG 4 cut(s) 825, 1655, 1727, 1797
MvaI CCWGG 4 cut(s) 825, 1655, 1727, 1797
MvnI CGCG 3 cut(s) 2442, 2525, 2716
MwoI GCNNNNNNNGC 4 cut(s) 575, 1201, 2147, 2585
NlaIV GGNNCC 5 cut(s) 246, 818, 819, 1722, 2071
NmuCI GTSAC 3 cut(s) 84, 127, 1593
NsiI ATGCAT 1 cut(s) 1956
NspI RCATGY 4 cut(s) 209, 506, 900, 1811
PaeI GCATGC 2 cut(s) 506, 1811
PciI ACATGT 1 cut(s) 896
PcsI WCGNNNNNNNCGW 1 cut(s) 236
PdmI GAANNNNTTC 1 cut(s) 176
PflMI CCANNNNNTGG 1 cut(s) 347
PkrI GCNGC 7 cut(s) 49, 508, 754, 1331, 1753, 2587, 2590
PleI GAGTC 3 cut(s) 933, 1780, 2273
PpsI GAGTC 3 cut(s) 933, 1780, 2273
PscI ACATGT 1 cut(s) 896
PsiI TTATAA 2 cut(s) 1038, 2084
Psp124BI GAGCTC 1 cut(s) 2628
Psp6I CCWGG 4 cut(s) 823, 1653, 1725, 1795
PspEI GGTNACC 1 cut(s) 84
PspGI CCWGG 4 cut(s) 823, 1653, 1725, 1795
PspN4I GGNNCC 5 cut(s) 246, 818, 819, 1722, 2071
PspOMI GGGCCC 1 cut(s) 817
PspPI GGNCC 4 cut(s) 405, 817, 818, 2012
PstI CTGCAG 2 cut(s) 757, 1846
PstNI CAGNNNCTG 1 cut(s) 278
PsuI RGATCY 3 cut(s) 352, 1860, 2069
PvuII CAGCTG 1 cut(s) 2672
RsaI GTAC 9 cut(s) 401, 465, 664, 980, 1166, 1175, 1436, 1681, 2066
RsaNI GTAC 9 cut(s) 400, 464, 663, 979, 1165, 1174, 1435, 1680, 2065
RseI CAYNNNNRTG 3 cut(s) 895, 1710, 2312
SacI GAGCTC 1 cut(s) 2628
SatI GCNGC 7 cut(s) 48, 507, 753, 1330, 1752, 2586, 2589
Sau96I GGNCC 4 cut(s) 405, 817, 818, 2012
ScaI AGTACT 2 cut(s) 465, 1166
SchI GAGTC 3 cut(s) 933, 1781, 2274
ScrFI CCNGG 4 cut(s) 825, 1655, 1727, 1797
SduI GDGCHC 6 cut(s) 821, 904, 1725, 2393, 2472, 2628
SexAI ACCWGGT 1 cut(s) 1795
SfaNI GCATC 5 cut(s) 773, 861, 1694, 1941, 1963
SfcI CTRYAG 2 cut(s) 753, 1842
SinI GGWCC 2 cut(s) 405, 2012
SmiMI CAYNNNNRTG 3 cut(s) 895, 1710, 2312
SmlI CTYRAG 6 cut(s) 965, 1145, 1466, 1505, 1589, 2241
SmoI CTYRAG 6 cut(s) 965, 1145, 1466, 1505, 1589, 2241
SphI GCATGC 2 cut(s) 506, 1811
SsiI CCGC 5 cut(s) 509, 1683, 2118, 2586, 2716
SspI AATATT 2 cut(s) 562, 1674
SspMI CTAG 2 cut(s) 2172, 2722
SstI GAGCTC 1 cut(s) 2628
StyD4I CCNGG 4 cut(s) 823, 1653, 1725, 1795
StyI CCWWGG 1 cut(s) 2335
TaaI ACNGT 6 cut(s) 268, 619, 847, 1388, 1417, 2371
TaiI ACGT 2 cut(s) 335, 2139
TaqI TCGA 9 cut(s) 55, 239, 483, 693, 704, 937, 1368, 1511, 2597
TatI WGTACW 6 cut(s) 399, 463, 662, 1164, 1173, 1434
TauI GCSGC 1 cut(s) 2588
TscAI CASTG 3 cut(s) 1851, 2029, 2374
TseFI GTSAC 3 cut(s) 84, 127, 1593
TseI GCWGC 6 cut(s) 47, 506, 752, 1329, 1751, 2588
Tsp45I GTSAC 3 cut(s) 84, 127, 1593
TspGWI ACGGA 1 cut(s) 2082
TspRI CASTG 3 cut(s) 1851, 2029, 2374
Van91I CCANNNNNTGG 1 cut(s) 347
Vha464I CTTAAG 1 cut(s) 1505
VpaK11BI GGWCC 2 cut(s) 405, 2012
XagI CCTNNNNNAGG 1 cut(s) 1658
XapI RAATTY 8 cut(s) 412, 583, 796, 1429, 1645, 1877, 2108, 2186
XceI RCATGY 4 cut(s) 209, 506, 900, 1811
XmnI GAANNNNTTC 1 cut(s) 176
XspI CTAG 2 cut(s) 2172, 2722
ZrmI AGTACT 2 cut(s) 465, 1166
Zsp2I ATGCAT 1 cut(s) 1956
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.