RLG00000022949

AAA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000279
Physical Location & Seq
Reverse (-)
15636910 .. 15703147
66238 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022949

Sequence Viewer

Length: 4113 bp
ATGGGGATGAAGAGTAAGCTGAAACAGGAACAAGTTACAGATACTAGCAGCCTAGTGCGTTTGGTATTCTCTTGGTCTATTGGGGATGTTCTCAATGAAAATCTTTACAGAAATCAGGTGCAGAGGATCCCCGATACATTTGTGACATTGACAAGTTACAAGAAATCATTCATTCCTTCACTTGTTGAGGAAACTCATGCTGATTTACTATCGAATATGAAGACTCTGTCACGTGCACCTACTTGTGAAATTCTGACAATCGAAGATTCAGCTGGATCTCCTGGTGACTTGTTTTACGATATAACGTATAACAGAGATACAGAAACTGATAAGAATCACAAAGGACCGATGTATGAGCCACAGGTTGGAGATATCATTGCCTTGACAAATGTTAGACCAAAATGCATCGATGATTTGAACAGGCCTCCAAGGTTCTATCTAATTGCTTATGTTGATAAAGCAAATGATATTGATGAATTTCCTGATGATCTCCGGATTCTCCCGTTCAAAATACTATCATCGAAGCCTATCAACTATGGAGAACCAGACATGCATAAGAGCAAGAGAGGAACACTTTTTGCTGTCTATCTTTTGAACTTGACAACAAATCTCCGTGTATGGAAGGCTTTGAACTCAGAAGTGGGAAATACAAATATCATTAGCAAAGTTCTGCAACCCAAGTCAGATGATGGGGATTCTTGTTCAGTTTGCTTCTCACAAGAAAAATGCAATACTGGCATTTCTGCTGTATGGCCTACAATTTGCTCTCAAAATCTAAATGAATCCCAAGAAGCTGCAGTTTTGAACTGTATCAGTTTGACTCAATGCCATCACCAGAATTCTGTAAAATTAATATGGGGTCCTCCTGGTACTGGCAAGACAAAGACAATGAGCGTGACACTCCTTGCTCTCCTTCAGTTAAAGTGCAGAACTCTCACATGCGCTCCCACCAATATTGCGGTGCTAGAAGTAGCAGCAAGACTCCGGAGATTGGTTAACCAGTCACTCGAGTATTGCAGGTATGGACTTGGAGATATAATTATCTTTGGTAACAAGAAGCGAATGAAGATTGACAACAATAATGATCTTCGTGACATATTTCTTGATCATCGTGTTAAAATCCTGATCAAGTGTTTGGTCCCTTTATCTGGGTGGAAACATTTGTTAGAATCAATGATACATTTAATTGATGATCCTGAGGAACAATATTCTTTGTATTTGGAAAAGAGAGCTGAAAAGCAGAAACAGAATGCACAGAAAAATGAGGAGGATAATCGAAAACGTAATGGAGATGAAGATTATCCGTTCACATTTGAGGAGTTTGTGAAGAAGGAATTTGATTCAATCAGTCAGGAGCTGAAGAAATGTATGGTAAATTTGTACACCCACTTACCAACTTCTTGCATTTCACTCGAAGTGGTGAAGGACATGGTCAGAGCTTTGGATTTGCTCAACTCTATTGAATCTTCATTGCATACTATTGGTGTTGCTAATGAAGAGTTGAAGTTAATCAAAGATTTCAAAGTTCCAGGAAGCGTTATTGGTTGCCTTATGCAGTTCAGAACAGAGTGTACAAACACACTAAAGTCGCTTCCAATGGAATTCTCTGTTCCTATTAACAAATATGCATTAGAGAACTTCTGCTTAGAAAATGCTTGCTTAATATTCTGTACTGCCTCAACTTCCTCCAAATTGCATGTTGTAAAAGGGACAAGACCGTTGGAATTGTTGGTCATTGATGAAGCTGCTCAGCTTAAAGAATGTGAATCAGCAATTCCTTTACAACTATCTGGTCTCCGTCATGCTATCCTTGTAGGAGATGAGAGGCAACTCCCTGCCATGGTTGAAAGCAAGATTGCAGCAAGTGCTGATTTTGGAAGAAGTTTGTTTGGAAGGCTGGCAAAGCTGGGACACAAGAAGCACCTACTCAATGTCCAGTACAGGATGCATCCATCCATCAGTTTATTTCCGAAAAGGGAGTTCTACGACAACCAGATAGTAGATGGTCCAAATGTCAAAGAAAGAAGCTATGAGAGGTGCTTCCTCATGGGAAAAATGTACCAATCCTATTCCTTCATAAATGTAGCCAATGGAAAAGAAGAATTTGATCACAGATTTAGTCAGAAAAATGTGGTGGAGGTTGCTGTAGTCTCTGGGATAGTAGCAAGCCTTTATAAAGAATTTATTGGAACAAAGAAGAAAGTTAGTATTGGGGTCATATCACCATACAAGGCTCAAGTTTATGCAATTCAAGAGATACTCAGAAAATATAATGAAACTTCTGATACTGGATTCTCCGTAAGTGTGCGATCTGTTGATGGGTTCCAAGGTGGTGAAGAAGATGTGATAATTATATCTACTGTCCGATGTAATGGGAACGGGTCAGTTGGTTTCTTGTCGAACCAGCAAAGAGCAAATGTTGCCTTAACACGTGCAAGGTATTGCCTTTGGATATTGGGGAATGCGTCAACTTTGATTACTAGTGACTCTGTTTGGAAGAAGCTAGTCCTTGATGCCAAGAAACGGAACTGTTTTTATAATGCTGATGAGGACAGCAACTTGGCTCAGGCTATTACAGCTGCCCTGTTGGAGCTTGACCAACTTCATTCTCTGCTTAATGTTGACTCTCTGCTGTTCAAAAATGCTATATGGAAGGTTTGCTTCACTCATGACTTTTTGAACTCCATAACAAAAATTGAAGACACTGTGATTCTTTGGGAAGTGCTTGCGTTATTAACCAAGCTTTCGAGTGGATGGCGCCGACCTCTTAAGGAGAAAGGAACTATAGTGTATGATGGGACTTCTGCTCAACTGTTAGAGAAGTATAAAATAAAAGGGAACTTGAATCTCATTTGGACTGTAGATATTCTCCAGGAGAATGCACATTACATCCAAGTAATGAAGTTTTGGGATATTTTGCCATTTTCTCATATACCAGAACTAGCAAAGCGTCTGGACATTGTTTTTGGGAATTTTACAGTGGACAAGATGAATCGCTGCCGTCACAAATGCATTGACAGGGATACTGTTGTTCCAATGAGATGGCCGGTGGTTTTCAGCAATTTCCCTATAGCTGATCATGAGGAGTTCCTTTCAAAACCATTGTCTTCTTTCAGTATAACAACTAATCGAGAAACAGCAACTTCAACATATGGGGAGACAGCAAAAGCAAAAAAAACAATTATCTCTTCAAAAAGCAATGTCAATAAAAAAGAAAGACGACTATGGAAAATAAAGGGTGAAGGAGTGAAGGATTTCAACACTTGCCTTGAAGCTAACCCTTCGGAGTTCTTGCCAAAACCATTATCATCACTAAGTCTAGCAGATAAGCCAGAAGCATCTACTTCCACTGACATGGAGACCGGGAAAGCAGTAAAACGATTTATCCCTTCGAAAAGCAATGTCAATCGAAAAGAAAGATTGTTATGGAAAATAAAAGGTAAAGGAGTGAAGGATTTCAGCACTTGCGTTGAAGCTAACCCTGTGGACTTCTTGTCAAAGCCATTTTCATCTCCAAGTCTATCAGATAAGCCAGAAGCATCTACTTCCACTGACATGGAGACAGCGAAAGCAGTAAATCCAATTATCTCTTCGAAAAGCAATGTCAATCAAAAGGAAAAATTGTTATGGAAGATAAAGGGTGAAGAAGAAGTGAAGGATTTCAGCACTTGCGTTGAAGCTATCCCTGTGGAGTTCTTGCCAAAACCATTATCATCACTGAGTCTAGCAGATAAGCCAGAAGCATCTACTTCCACTGACATGGAGACAGTGAAAGCAGTAAAACCAATTATCCCTTCGAAAAGCAATGCCAATCAAAAAGAAAGATTGTTATGGAAGATAAAGGGTGAAGAAGGAGTGAAGGATTTCAGCACTTGCCTTGAAGCTAACCCTGTGGAGTTGTTGTCAAAACCATTATCATCACTATGTCTAGCAGATAAGCCAGAAGCATCTACTTCCACTGACATGGCTTCAATCGACGACCTTGTCGCGGGAACGAGATTGGGCTTACACATGTCCATGGATTCAGAAACGGCCGAATCGCAAAATCATGTCCCATATGGCCACGTCAATAGGCAGCTCGGTGGAGACTGTGGCGTCAAGCAGCTCCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12

Protein Analysis

1371

Amino Acids

153.92

Weight (kDa)

8.22

Isoelectric Point (pI)

37.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 80 - 211 9.5e-13 Domain of unknown function (DUF6469)
AAA_11 PF13086 258 - 429 6e-15 AAA domain
AAA_11 PF13086 543 - 618 2.4e-15 AAA domain
AAA_12 PF13087 626 - 822 7.2e-57 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000279)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65810 AT1G65810 AT1G65810 AT4G05540 AT5G37140 AT5G37150 AT5G37160 AT5G37165 AT5G52090
fragaria_vesca FvH4_1g27541 FvH4_1g27542 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_3g00170 FvH4_3g00170 FvH4_3g00170 FvH4_5g05930 FvH4_5g05930
malus_domestica MD08G1077700.v1.1 MD08G1077900.v1.1 MD08G1078000.v1.1 MD08G1078100.v1.1 MD08G1078300.v1.1 MD09G1057800.v1.1 MD09G1281400.v1.1 MD11G1222900.v1.1 MD14G1146400.v1.1
prunus_persica Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G202100_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.3G013100_v2.0.a1
pyrus_communis pycom09g18900 pycom09g18910 pycom13g26720 pycom13g26730 pycom13g26760 pycom13g26770
rosa_chinensis RchiOBHm_Chr3g0488001 RchiOBHm_Chr3g0488011 RchiOBHm_Chr3g0488031 RchiOBHm_Chr3g0488061 RchiOBHm_Chr3g0488111 RchiOBHm_Chr3g0488201 RchiOBHm_Chr3g0488211 RchiOBHm_Chr3g0488221 RchiOBHm_Chr3g0488321 RchiOBHm_Chr3g0488431 RchiOBHm_Chr6g0264601 RchiOBHm_Chr6g0264641
rosa_laevigata RLG00000014240 RLG00000014241 RLG00000022910 RLG00000022949 RLG00000030376
rosa_multiflora Rmu_co8075388.1_g000001 Rmu_co8424869.1_g000001 Rmu_co8427783.1_g000001 Rmu_sc0000546.1_g000002 Rmu_sc0000546.1_g000015 Rmu_sc0011052.1_g000014 Rmu_sc0017772.1_g000004
rosa_roxburghii Rroxscaffold_175G00431980 Rroxscaffold_175G00431990 Rroxscaffold_1G00034600 Rroxscaffold_6G00394230 Rroxscaffold_6G00394270 Rroxscaffold_6G00394280 Rroxscaffold_6G00394350 Rroxscaffold_7G00203240 Rroxscaffold_7G00203270 Rroxscaffold_7G00203280 Rroxscaffold_7G00203300 Rroxscaffold_7G00205740
rosa_rugosa Rorug03G0238000 Rorug03G0238100 Rorug03G0238100 Rorug03G0238500 Rorug05G0155400 Rorug05G0356000 Rorug05G0463000 Rorug06G0015200 Rorug06G0015300 Rorug06G0015400 Rorug06G0015500 Rorug06G0015500
rosa_samantha Rh1DG088100 Rh2AG244800 Rh2BG187500 Rh2CG247300 Rh3BG322700 Rh3BG322800 Rh3BG323100 Rh3BG323900 Rh3BG324000 Rh3BG324300 Rh3CG321300 Rh3CG321400 Rh3CG321500 Rh3DG319400 Rh3DG319900 Rh3DG320100 Rh3DG320200 Rh6AG133700 Rh6AG133800 Rh6AG133900 Rh6CG129300 Rh6CG129800 Rh6CG130200 Rh6DG116500 Rh6DG117200 Rh7DG378000
rosa_wichuraiana Rw2G014710 Rw3G025340 Rw3G025350 Rw3G025360 Rw5G020630 Rw6G011590 Rw6G011600 Rw7G041380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2175, 2538
AasI GACNNNNNNGTC 2 cut(s) 3983, 4094
Acc36I ACCTGC 1 cut(s) 1008
AccB1I GGYRCC 1 cut(s) 2757
AccB7I CCANNNNNTGG 1 cut(s) 365
AccII CGCG 1 cut(s) 3989
AccIII TCCGGA 2 cut(s) 492, 984
AciI CCGC 2 cut(s) 959, 3989
AclWI GGATC 4 cut(s) 121, 134, 283, 1187
AcoI YGGCCR 3 cut(s) 3044, 4032, 4060
AcsI RAATTY 9 cut(s) 249, 476, 838, 1334, 1375, 1601, 2102, 2180, 2971
AcuI CTGAAG 2 cut(s) 899, 1379
AcvI CACGTG 2 cut(s) 233, 2432
AcyI GRCGYC 2 cut(s) 2758, 4095
AfaI GTAC 6 cut(s) 871, 1382, 1573, 1672, 1940, 2060
AfiI CCNNNNNNNGG 7 cut(s) 365, 872, 991, 1148, 1840, 2523, 3988
AflII CTTAAG 1 cut(s) 2768
AflIII ACRYGT 2 cut(s) 2429, 4011
AhdI GACNNNNNGTC 1 cut(s) 3493
AhlI ACTAGT 1 cut(s) 2480
AjiI CACGTC 1 cut(s) 4066
AjnI CCWGG 4 cut(s) 280, 865, 1528, 2871
AjuI GAANNNNNNNTTGG 4 cut(s) 3574, 3606, 3778, 3810
Alw21I GWGCWC 1 cut(s) 238
Alw26I GTCTC 7 cut(s) 1799, 2155, 3152, 3353, 3554, 3758, 4080
Alw44I GTGCAC 1 cut(s) 234
AlwI GGATC 4 cut(s) 121, 134, 283, 1187
AlwNI CAGNNNCTG 2 cut(s) 326, 1357
Ama87I CYCGRG 1 cut(s) 1007
Aor13HI TCCGGA 2 cut(s) 492, 984
AoxI GGCC 5 cut(s) 422, 752, 3044, 4032, 4060
ApaLI GTGCAC 1 cut(s) 234
ApeKI GCWGC 9 cut(s) 48, 794, 974, 1745, 1859, 2579, 2997, 4075, 4102
ApoI RAATTY 9 cut(s) 249, 476, 838, 1334, 1375, 1601, 2102, 2180, 2971
ArsI GACNNNNNNTTYG 4 cut(s) 2948, 2980, 3089, 3121
AseI ATTAAT 1 cut(s) 851
Asp700I GAANNNNTTC 5 cut(s) 167, 3254, 3455, 3659, 3863
AspLEI GCGC 2 cut(s) 944, 2760
AspS9I GGNCC 4 cut(s) 344, 860, 1138, 2006
AsuC2I CCSGG 1 cut(s) 3364
AsuHPI GGTGA 8 cut(s) 296, 824, 1432, 2214, 2345, 3251, 3653, 3857
AsuII TTCGAA 3 cut(s) 3392, 3593, 3797
AvaI CYCGRG 1 cut(s) 1007
AvaII GGWCC 4 cut(s) 344, 860, 1138, 2006
AxyI CCTNAGG 1 cut(s) 1197
BaeGI GKGCMC 1 cut(s) 238
BaeI ACNNNNGTAYC 4 cut(s) 126, 159, 3015, 3048
BalI TGGCCA 1 cut(s) 4062
BamHI GGATCC 1 cut(s) 126
BanI GGYRCC 1 cut(s) 2757
BbrPI CACGTG 2 cut(s) 233, 2432
BbsI GAAGAC 3 cut(s) 227, 2706, 3099
Bbv12I GWGCWC 1 cut(s) 238
BbvI GCAGC 8 cut(s) 60, 781, 986, 1732, 1871, 2566, 2984, 4087
BccI CCATC 9 cut(s) 683, 837, 1960, 1964, 1997, 2312, 2748, 2789, 3036
BceAI ACGGC 2 cut(s) 2985, 4047
BcgI CGANNNNNNTGC 2 cut(s) 1393, 1427
BciT130I CCWGG 4 cut(s) 282, 867, 1530, 2873
BciVI GTATCC 1 cut(s) 3016
BclI TGATCA 4 cut(s) 1105, 1125, 2107, 3076
BcnI CCSGG 1 cut(s) 3364
BcoDI GTCTC 7 cut(s) 1799, 2155, 3152, 3353, 3554, 3758, 4080
BcuI ACTAGT 1 cut(s) 2480
BfaI CTAG 9 cut(s) 45, 53, 965, 2481, 2504, 2942, 3320, 3725, 3929
BfmI CTRYAG 5 cut(s) 795, 2145, 2784, 2859, 3069
BfoI RGCGCY 1 cut(s) 2761
BfrI CTTAAG 1 cut(s) 2768
BfuAI ACCTGC 1 cut(s) 1008
BfuI GTATCC 1 cut(s) 3016
BisI GCNGC 9 cut(s) 49, 795, 975, 1746, 1860, 2580, 2998, 4076, 4103
BlpI GCTNAGC 1 cut(s) 1749
BlsI GCNGC 9 cut(s) 50, 796, 976, 1747, 1861, 2581, 2999, 4077, 4104
Bme1390I CCNGG 5 cut(s) 282, 867, 1530, 2873, 3364
Bme18I GGWCC 4 cut(s) 344, 860, 1138, 2006
BmeRI GACNNNNNGTC 1 cut(s) 3493
BmeT110I CYCGRG 1 cut(s) 1007
BmgBI CACGTC 1 cut(s) 4066
BmgT120I GGNCC 4 cut(s) 344, 860, 1138, 2006
BmiI GGNNCC 5 cut(s) 128, 861, 1140, 2324, 2759
BmrFI CCNGG 5 cut(s) 282, 867, 1530, 2873, 3364
BmsI GCATC 8 cut(s) 414, 1935, 1957, 2503, 3347, 3548, 3752, 3956
BpiI GAAGAC 3 cut(s) 227, 2706, 3099
BpmI CTGGAG 2 cut(s) 2855, 4091
Bpu10I CCTNAGC 1 cut(s) 2565
Bpu1102I GCTNAGC 1 cut(s) 1749
Bpu14I TTCGAA 3 cut(s) 3392, 3593, 3797
BpuEI CTTGAG 1 cut(s) 2220
BpuMI CCSGG 1 cut(s) 3364
Bsa29I ATCGAT 1 cut(s) 408
BsaAI YACGTR 2 cut(s) 233, 2432
BsaBI GATNNNNATC 1 cut(s) 333
BsaHI GRCGYC 2 cut(s) 2758, 4095
BsaI GGTCTC 2 cut(s) 1799, 3353
BsaJI CCNNGG 4 cut(s) 428, 1839, 2326, 4017
BsaWI WCCGGW 2 cut(s) 492, 984
BsaXI ACNNNNNCTCC 4 cut(s) 928, 958, 3887, 3917
Bsc4I CCNNNNNNNGG 7 cut(s) 365, 872, 991, 1148, 1840, 2523, 3988
Bse118I RCCGGY 1 cut(s) 3046
Bse1I ACTGG 6 cut(s) 739, 877, 1000, 1936, 2293, 4108
Bse21I CCTNAGG 1 cut(s) 1197
Bse3DI GCAATG 6 cut(s) 375, 1469, 3205, 3406, 3607, 3811
Bse8I GATNNNNATC 1 cut(s) 333
BseAI TCCGGA 2 cut(s) 492, 984
BseBI CCWGG 4 cut(s) 282, 867, 1530, 2873
BseCI ATCGAT 1 cut(s) 408
BseDI CCNNGG 4 cut(s) 428, 1839, 2326, 4017
BseGI GGATG 7 cut(s) 12, 91, 1948, 1950, 1952, 2759, 2889
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 7 cut(s) 365, 872, 991, 1148, 1840, 2523, 3988
BseMI GCAATG 6 cut(s) 375, 1469, 3205, 3406, 3607, 3811
BseMII CTCAG 6 cut(s) 648, 1188, 1763, 2275, 2579, 3710
BseNI ACTGG 6 cut(s) 739, 877, 1000, 1936, 2293, 4108
BseRI GAGGAG 3 cut(s) 1280, 1331, 3098
BseSI GKGCMC 1 cut(s) 238
BseX3I CGGCCG 1 cut(s) 4032
BseXI GCAGC 8 cut(s) 60, 781, 986, 1732, 1871, 2566, 2984, 4087
BseYI CCCAGC 1 cut(s) 1906
BsgI GTGCAG 2 cut(s) 140, 946
Bsh1236I CGCG 1 cut(s) 3989
Bsh1285I CGRYCG 1 cut(s) 4035
BshFI GGCC 5 cut(s) 424, 754, 3046, 4034, 4062
BshNI GGYRCC 1 cut(s) 2757
BshVI ATCGAT 1 cut(s) 408
BsiEI CGRYCG 1 cut(s) 4035
BsiHKAI GWGCWC 1 cut(s) 238
BsiHKCI CYCGRG 1 cut(s) 1007
BsiSI CCGG 4 cut(s) 493, 985, 3047, 3363
BslFI GGGAC 5 cut(s) 1124, 1723, 1923, 2812, 4037
BslI CCNNNNNNNGG 7 cut(s) 365, 872, 991, 1148, 1840, 2523, 3988
BsmAI GTCTC 7 cut(s) 1799, 2155, 3152, 3353, 3554, 3758, 4080
BsmFI GGGAC 5 cut(s) 1124, 1723, 1923, 2812, 4037
BsmI GAATGC 3 cut(s) 1255, 2467, 2884
BsnI GGCC 5 cut(s) 424, 754, 3046, 4034, 4062
Bso31I GGTCTC 2 cut(s) 1799, 3353
BsoBI CYCGRG 1 cut(s) 1007
Bsp119I TTCGAA 3 cut(s) 3392, 3593, 3797
Bsp1286I GDGCHC 1 cut(s) 238
Bsp13I TCCGGA 2 cut(s) 492, 984
Bsp1407I TGTACA 2 cut(s) 1380, 1571
Bsp1720I GCTNAGC 1 cut(s) 1749
Bsp19I CCATGG 2 cut(s) 1839, 4017
BspACI CCGC 2 cut(s) 959, 3989
BspANI GGCC 5 cut(s) 424, 754, 3046, 4034, 4062
BspCNI CTCAG 6 cut(s) 647, 1189, 1762, 2274, 2578, 3711
BspDI ATCGAT 1 cut(s) 408
BspEI TCCGGA 2 cut(s) 492, 984
BspFNI CGCG 1 cut(s) 3989
BspHI TCATGA 2 cut(s) 2668, 3079
BspLI GGNNCC 5 cut(s) 128, 861, 1140, 2324, 2759
BspMAI CTGCAG 1 cut(s) 799
BspMI ACCTGC 1 cut(s) 1008
BspPI GGATC 4 cut(s) 121, 134, 283, 1187
BspT104I TTCGAA 3 cut(s) 3392, 3593, 3797
BspT107I GGYRCC 1 cut(s) 2757
BspTI CTTAAG 1 cut(s) 2768
BspTNI GGTCTC 2 cut(s) 1799, 3353
BsrDI GCAATG 6 cut(s) 375, 1469, 3205, 3406, 3607, 3811
BsrFI RCCGGY 1 cut(s) 3046
BsrGI TGTACA 2 cut(s) 1380, 1571
BsrI ACTGG 6 cut(s) 739, 877, 1000, 1936, 2293, 4108
BssAI RCCGGY 1 cut(s) 3046
BssECI CCNNGG 4 cut(s) 428, 1839, 2326, 4017
BssNI GRCGYC 2 cut(s) 2758, 4095
BssT1I CCWWGG 4 cut(s) 428, 1839, 2326, 4017
Bst2UI CCWGG 4 cut(s) 282, 867, 1530, 2873
Bst6I CTCTTC 4 cut(s) 5, 1491, 3193, 3595
BstACI GRCGYC 2 cut(s) 2758, 4095
BstAFI CTTAAG 1 cut(s) 2768
BstAPI GCANNNNNTGC 2 cut(s) 1865, 2420
BstAUI TGTACA 2 cut(s) 1380, 1571
BstBAI YACGTR 2 cut(s) 233, 2432
BstBI TTCGAA 3 cut(s) 3392, 3593, 3797
BstC8I GCNNGC 4 cut(s) 1657, 1899, 2167, 2727
BstDEI CTNAG 8 cut(s) 634, 1197, 1645, 1749, 2261, 2565, 3314, 3719
BstDSI CCRYGG 2 cut(s) 1839, 4017
BstF5I GGATG 7 cut(s) 12, 91, 1948, 1950, 1952, 2759, 2889
BstFNI CGCG 1 cut(s) 3989
BstH2I RGCGCY 1 cut(s) 2761
BstHHI GCGC 2 cut(s) 944, 2760
BstMAI GTCTC 7 cut(s) 1799, 2155, 3152, 3353, 3554, 3758, 4080
BstMCI CGRYCG 1 cut(s) 4035
BstMWI GCNNNNNNNGC 5 cut(s) 735, 1865, 1903, 2420, 2576
BstNI CCWGG 4 cut(s) 282, 867, 1530, 2873
BstNSI RCATGY 4 cut(s) 553, 942, 1700, 4015
BstSCI CCNGG 5 cut(s) 280, 865, 1528, 2871, 3362
BstSFI CTRYAG 5 cut(s) 795, 2145, 2784, 2859, 3069
BstSLI GKGCMC 1 cut(s) 238
BstUI CGCG 1 cut(s) 3989
BstV1I GCAGC 8 cut(s) 60, 781, 986, 1732, 1871, 2566, 2984, 4087
BstV2I GAAGAC 3 cut(s) 227, 2706, 3099
BstX2I RGATCY 2 cut(s) 126, 275
BstXI CCANNNNNNTGG 5 cut(s) 3042, 3355, 3556, 3760, 3964
BstYI RGATCY 2 cut(s) 126, 275
BstZI CGGCCG 1 cut(s) 4032
Bsu15I ATCGAT 1 cut(s) 408
Bsu36I CCTNAGG 1 cut(s) 1197
BsuI GTATCC 1 cut(s) 3016
BsuRI GGCC 5 cut(s) 424, 754, 3046, 4034, 4062
BsuTUI ATCGAT 1 cut(s) 408
BtgI CCRYGG 2 cut(s) 1839, 4017
BtrI CACGTC 1 cut(s) 4066
BtsCI GGATG 7 cut(s) 12, 91, 1948, 1950, 1952, 2759, 2889
BtsIMutI CAGTG 8 cut(s) 2703, 2985, 3348, 3549, 3716, 3753, 3774, 3957
BveI ACCTGC 1 cut(s) 1008
Cac8I GCNNGC 4 cut(s) 1657, 1899, 2167, 2727
CaiI CAGNNNCTG 2 cut(s) 326, 1357
CciI TCATGA 2 cut(s) 2668, 3079
CfoI GCGC 2 cut(s) 944, 2760
Cfr10I RCCGGY 1 cut(s) 3046
Cfr13I GGNCC 4 cut(s) 344, 860, 1138, 2006
ClaI ATCGAT 1 cut(s) 408
CseI GACGC 3 cut(s) 2454, 2939, 4084
Csp6I GTAC 6 cut(s) 870, 1381, 1572, 1671, 1939, 2059
CspCI CAANNNNNGTGG 2 cut(s) 937, 972
CviQI GTAC 6 cut(s) 870, 1381, 1572, 1671, 1939, 2059
DdeI CTNAG 8 cut(s) 634, 1197, 1645, 1749, 2261, 2565, 3314, 3719
DinI GGCGCC 1 cut(s) 2759
DrdI GACNNNNNNGTC 2 cut(s) 3983, 4094
DriI GACNNNNNGTC 1 cut(s) 3493
DseDI GACNNNNNNGTC 2 cut(s) 3983, 4094
EaeI YGGCCR 3 cut(s) 3044, 4032, 4060
EagI CGGCCG 1 cut(s) 4032
Eam1104I CTCTTC 4 cut(s) 5, 1491, 3193, 3595
Eam1105I GACNNNNNGTC 1 cut(s) 3493
EarI CTCTTC 4 cut(s) 5, 1491, 3193, 3595
EclXI CGGCCG 1 cut(s) 4032
Eco130I CCWWGG 4 cut(s) 428, 1839, 2326, 4017
Eco147I AGGCCT 1 cut(s) 424
Eco31I GGTCTC 2 cut(s) 1799, 3353
Eco32I GATATC 1 cut(s) 373
Eco47I GGWCC 4 cut(s) 344, 860, 1138, 2006
Eco52I CGGCCG 1 cut(s) 4032
Eco57I CTGAAG 2 cut(s) 899, 1379
Eco72I CACGTG 2 cut(s) 233, 2432
Eco81I CCTNAGG 1 cut(s) 1197
Eco88I CYCGRG 1 cut(s) 1007
EcoO109I RGGNCCY 1 cut(s) 860
EcoRI GAATTC 2 cut(s) 838, 1601
EcoRII CCWGG 4 cut(s) 280, 865, 1528, 2871
EcoRV GATATC 1 cut(s) 373
EcoT14I CCWWGG 4 cut(s) 428, 1839, 2326, 4017
EcoT22I ATGCAT 5 cut(s) 407, 555, 1630, 1950, 3014
EgeI GGCGCC 1 cut(s) 2759
EheI GGCGCC 1 cut(s) 2759
ErhI CCWWGG 4 cut(s) 428, 1839, 2326, 4017
FalI AAGNNNNNCTT 2 cut(s) 2645, 2677
FaqI GGGAC 5 cut(s) 1124, 1723, 1923, 2812, 4037
FauI CCCGC 1 cut(s) 3982
FauNDI CATATG 2 cut(s) 3151, 4057
FbaI TGATCA 4 cut(s) 1105, 1125, 2107, 3076
Fnu4HI GCNGC 9 cut(s) 49, 795, 975, 1746, 1860, 2580, 2998, 4076, 4103
FokI GGATG 7 cut(s) 19, 98, 1935, 1939, 1957, 2766, 2876
Fsp4HI GCNGC 9 cut(s) 49, 795, 975, 1746, 1860, 2580, 2998, 4076, 4103
FspBI CTAG 9 cut(s) 45, 53, 965, 2481, 2504, 2942, 3320, 3725, 3929
GlaI GCGC 2 cut(s) 943, 2759
GluI GCNGC 9 cut(s) 49, 795, 975, 1746, 1860, 2580, 2998, 4076, 4103
GsaI CCCAGC 1 cut(s) 1910
GsuI CTGGAG 2 cut(s) 2855, 4091
HaeII RGCGCY 1 cut(s) 2761
HaeIII GGCC 5 cut(s) 424, 754, 3046, 4034, 4062
HapII CCGG 4 cut(s) 493, 985, 3047, 3363
HgaI GACGC 3 cut(s) 2454, 2939, 4084
HhaI GCGC 2 cut(s) 944, 2760
Hin1I GRCGYC 2 cut(s) 2758, 4095
Hin6I GCGC 2 cut(s) 942, 2758
HinP1I GCGC 2 cut(s) 942, 2758
HincII GTYRAC 3 cut(s) 997, 2469, 2623
HindII GTYRAC 3 cut(s) 997, 2469, 2623
HindIII AAGCTT 1 cut(s) 2741
HpaI GTTAAC 1 cut(s) 997
HpaII CCGG 4 cut(s) 493, 985, 3047, 3363
HphI GGTGA 8 cut(s) 296, 824, 1432, 2214, 2345, 3251, 3653, 3857
Hpy166II GTNNAC 9 cut(s) 236, 997, 1308, 1383, 1572, 2469, 2623, 2983, 3487
Hpy8I GTNNAC 9 cut(s) 236, 997, 1308, 1383, 1572, 2469, 2623, 2983, 3487
Hpy99I CGWCG 1 cut(s) 3980
HpyCH4IV ACGT 5 cut(s) 232, 305, 1282, 2431, 4065
HpyF10VI GCNNNNNNNGC 5 cut(s) 735, 1865, 1903, 2420, 2576
HpyF3I CTNAG 8 cut(s) 634, 1197, 1645, 1749, 2261, 2565, 3314, 3719
HpySE526I ACGT 5 cut(s) 232, 305, 1282, 2431, 4065
Hsp92I GRCGYC 2 cut(s) 2758, 4095
HspAI GCGC 2 cut(s) 942, 2758
KasI GGCGCC 1 cut(s) 2757
Kpn2I TCCGGA 2 cut(s) 492, 984
Ksp22I TGATCA 4 cut(s) 1105, 1125, 2107, 3076
KspAI GTTAAC 1 cut(s) 997
LmnI GCTCC 4 cut(s) 949, 1354, 2590, 4110
Lsp1109I GCAGC 8 cut(s) 60, 781, 986, 1732, 1871, 2566, 2984, 4087
LweI GCATC 8 cut(s) 414, 1935, 1957, 2503, 3347, 3548, 3752, 3956
MaeI CTAG 9 cut(s) 45, 53, 965, 2481, 2504, 2942, 3320, 3725, 3929
MaeII ACGT 5 cut(s) 232, 305, 1282, 2431, 4065
MflI RGATCY 2 cut(s) 126, 275
MhlI GDGCHC 1 cut(s) 238
MlsI TGGCCA 1 cut(s) 4062
MluNI TGGCCA 1 cut(s) 4062
Mly113I GGCGCC 1 cut(s) 2758
MlyI GAGTC 6 cut(s) 217, 814, 975, 2480, 2618, 3730
MmeI TCCRAC 3 cut(s) 346, 1701, 2568
Mox20I TGGCCA 1 cut(s) 4062
Mph1103I ATGCAT 5 cut(s) 407, 555, 1630, 1950, 3014
MroI TCCGGA 2 cut(s) 492, 984
MroXI GAANNNNTTC 5 cut(s) 167, 3254, 3455, 3659, 3863
MscI TGGCCA 1 cut(s) 4062
MslI CAYNNNNRTG 6 cut(s) 3353, 3554, 3758, 3922, 3962, 4016
Msp20I TGGCCA 1 cut(s) 4062
MspA1I CMGCKG 2 cut(s) 272, 2579
MspCI CTTAAG 1 cut(s) 2768
MspI CCGG 4 cut(s) 493, 985, 3047, 3363
MspR9I CCNGG 5 cut(s) 282, 867, 1530, 2873, 3364
Mva1269I GAATGC 3 cut(s) 1255, 2467, 2884
MvaI CCWGG 4 cut(s) 282, 867, 1530, 2873
MvnI CGCG 1 cut(s) 3989
MwoI GCNNNNNNNGC 5 cut(s) 735, 1865, 1903, 2420, 2576
NarI GGCGCC 1 cut(s) 2758
NciI CCSGG 1 cut(s) 3364
NcoI CCATGG 2 cut(s) 1839, 4017
NdeI CATATG 2 cut(s) 3151, 4057
NlaIV GGNNCC 5 cut(s) 128, 861, 1140, 2324, 2759
NmuCI GTSAC 8 cut(s) 142, 228, 284, 895, 1002, 1091, 2483, 3002
NsiI ATGCAT 5 cut(s) 407, 555, 1630, 1950, 3014
NspI RCATGY 4 cut(s) 553, 942, 1700, 4015
NspV TTCGAA 3 cut(s) 3392, 3593, 3797
PaeR7I CTCGAG 1 cut(s) 1007
PagI TCATGA 2 cut(s) 2668, 3079
PceI AGGCCT 1 cut(s) 424
PciI ACATGT 1 cut(s) 4011
PctI GAATGC 3 cut(s) 1255, 2467, 2884
PdmI GAANNNNTTC 5 cut(s) 167, 3254, 3455, 3659, 3863
PflFI GACNNNGTC 3 cut(s) 226, 1430, 3983
PflMI CCANNNNNTGG 1 cut(s) 365
PfoI TCCNGGA 2 cut(s) 1528, 2871
PkrI GCNGC 9 cut(s) 50, 796, 976, 1747, 1861, 2581, 2999, 4077, 4104
PleI GAGTC 6 cut(s) 217, 814, 975, 2480, 2618, 3729
PluTI GGCGCC 1 cut(s) 2761
PmaCI CACGTG 2 cut(s) 233, 2432
PmlI CACGTG 2 cut(s) 233, 2432
PpsI GAGTC 6 cut(s) 217, 814, 975, 2480, 2618, 3729
Ppu21I YACGTR 2 cut(s) 233, 2432
PpuMI RGGWCCY 1 cut(s) 860
PscI ACATGT 1 cut(s) 4011
PshBI ATTAAT 1 cut(s) 851
PsiI TTATAA 2 cut(s) 2175, 2538
Psp5II RGGWCCY 1 cut(s) 860
Psp6I CCWGG 4 cut(s) 280, 865, 1528, 2871
PspCI CACGTG 2 cut(s) 233, 2432
PspFI CCCAGC 1 cut(s) 1906
PspGI CCWGG 4 cut(s) 280, 865, 1528, 2871
PspN4I GGNNCC 5 cut(s) 128, 861, 1140, 2324, 2759
PspPI GGNCC 4 cut(s) 344, 860, 1138, 2006
PspPPI RGGWCCY 1 cut(s) 860
PspXI VCTCGAGB 1 cut(s) 1007
PsrI GAACNNNNNNTAC 2 cut(s) 1555, 1587
PstI CTGCAG 1 cut(s) 799
PstNI CAGNNNCTG 2 cut(s) 326, 1357
PsuI RGATCY 2 cut(s) 126, 275
PsyI GACNNNGTC 3 cut(s) 226, 1430, 3983
PvuII CAGCTG 2 cut(s) 272, 2579
RsaI GTAC 6 cut(s) 871, 1382, 1573, 1672, 1940, 2060
RsaNI GTAC 6 cut(s) 870, 1381, 1572, 1671, 1939, 2059
RseI CAYNNNNRTG 6 cut(s) 3353, 3554, 3758, 3922, 3962, 4016
SatI GCNGC 9 cut(s) 49, 795, 975, 1746, 1860, 2580, 2998, 4076, 4103
Sau96I GGNCC 4 cut(s) 344, 860, 1138, 2006
SchI GAGTC 6 cut(s) 217, 814, 975, 2480, 2618, 3730
ScrFI CCNGG 5 cut(s) 282, 867, 1530, 2873, 3364
SduI GDGCHC 1 cut(s) 238
SfaNI GCATC 8 cut(s) 414, 1935, 1957, 2503, 3347, 3548, 3752, 3956
SfcI CTRYAG 5 cut(s) 795, 2145, 2784, 2859, 3069
SfoI GGCGCC 1 cut(s) 2759
Sfr274I CTCGAG 1 cut(s) 1007
SfuI TTCGAA 3 cut(s) 3392, 3593, 3797
SinI GGWCC 4 cut(s) 344, 860, 1138, 2006
SlaI CTCGAG 1 cut(s) 1007
SmiMI CAYNNNNRTG 6 cut(s) 3353, 3554, 3758, 3922, 3962, 4016
SmlI CTYRAG 3 cut(s) 1007, 2235, 2768
SmoI CTYRAG 3 cut(s) 1007, 2235, 2768
SpeI ACTAGT 1 cut(s) 2480
SseBI AGGCCT 1 cut(s) 424
SsiI CCGC 2 cut(s) 959, 3989
SspDI GGCGCC 1 cut(s) 2757
SspI AATATT 3 cut(s) 955, 1208, 1665
SspMI CTAG 9 cut(s) 45, 53, 965, 2481, 2504, 2942, 3320, 3725, 3929
StuI AGGCCT 1 cut(s) 424
StyD4I CCNGG 5 cut(s) 280, 865, 1528, 2871, 3362
StyI CCWWGG 4 cut(s) 428, 1839, 2326, 4017
TaiI ACGT 5 cut(s) 235, 308, 1285, 2434, 4068
TaqII GACCGA 1 cut(s) 361
TatI WGTACW 4 cut(s) 1380, 1571, 1670, 1938
TscAI CASTG 8 cut(s) 2710, 2985, 3355, 3556, 3723, 3760, 3774, 3964
TseFI GTSAC 8 cut(s) 142, 228, 284, 895, 1002, 1091, 2483, 3002
TseI GCWGC 9 cut(s) 48, 794, 974, 1745, 1859, 2579, 2997, 4075, 4102
Tsp45I GTSAC 8 cut(s) 142, 228, 284, 895, 1002, 1091, 2483, 3002
TspGWI ACGGA 5 cut(s) 602, 1293, 1787, 2287, 2539
TspRI CASTG 8 cut(s) 2710, 2985, 3355, 3556, 3723, 3760, 3774, 3964
Tth111I GACNNNGTC 3 cut(s) 226, 1430, 3983
Van91I CCANNNNNTGG 1 cut(s) 365
Vha464I CTTAAG 1 cut(s) 2768
VneI GTGCAC 1 cut(s) 234
VpaK11BI GGWCC 4 cut(s) 344, 860, 1138, 2006
VspI ATTAAT 1 cut(s) 851
XapI RAATTY 9 cut(s) 249, 476, 838, 1334, 1375, 1601, 2102, 2180, 2971
XceI RCATGY 4 cut(s) 553, 942, 1700, 4015
XcmI CCANNNNNNNNNTGG 1 cut(s) 2000
XhoI CTCGAG 1 cut(s) 1007
XmnI GAANNNNTTC 5 cut(s) 167, 3254, 3455, 3659, 3863
XspI CTAG 9 cut(s) 45, 53, 965, 2481, 2504, 2942, 3320, 3725, 3929
Zsp2I ATGCAT 5 cut(s) 407, 555, 1630, 1950, 3014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.