Rh3CG321400

AAA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0000279
Physical Location & Seq
Forward (+)
34575517 .. 34605518
30002 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG321400.1

Sequence Viewer

Length: 3924 bp
ATGGGGAAGAAGAGTAAGCTGAAACAGGAACTAGTTACAGATACTAGCAGCCTACTGGGTTTGGTATTCTCTTGGTCTATTTCGGATGTTCTCAATGAAAATCTTTACAGAAATCAGGTGCAGGGGATCCCTGACACATTTGTGACATCTACGAGTTACAGGAAATCATTCATTCCTTCACTTCTTGAGGAAACTCATGCTGATTTACTATCGAATATGAAGACTCTGTCACATGCACCTACTTGTGAAATACTGTCAATCGAAGATTCAGCTAGACCTCCTGGTGACTTGTTTTACGATATAATGTATAATAGAGATACAGAAACTGATGAGAATCACAAAGGACCGATGTATGAGCCACAGGTTGGAGATATCATTGCCTTGACAAATGTTAGACCAAATTGCATTGATGATTTGAACAGGCCTCCAAGGTTCTATCTAATCGCTTATGTTCATAAAGCAAATGATATTGATGAATTTCCTGATGATCTCCAGTTCAAAATACTATCATCGAAGCCTATCAACTATGGAGAACCAGATATGCGTAAGAGCAATAGAGAAACACTTTTTGCTGTCTATCTTATGAACTTGATTACAAATCTCCGTGTATGGAAGGCTTTGAACTCAGAGGGAAATACAAATATCATTAACAAAGTTCTGCAACCCAAGTCAGATGATGGGGATTCTTGTTCAGTTTGCTTCCCCAGGGAAAAATCCTACACTGGCATTTCTGCTATATGGCCTACAATTTGCTCTCAAAATCTAAATGAATCCCAAGAAGCTGCGGTTTTGAACTGTATCAGTGTGGCTCAATGCCATCACCAGAATTCTGTAAAATTAATATGGGGTCCTCCTGGTACTGGCAAGACGAAGACAATGAGTCTGACACTCTTTGCTCTCTTTCATTTAAAGTGCAGAACTCTCACATGTGCTCCCACCAATATTGCGGTGCTAGAAGTAGCAGCAAGACTCCGGAGATTGGTTAACCAGTCACTCGAGTACGGCAGGTATGGACTAGGAGATATAGTTCTCTTTGGTAACAAGAAGCGAATGAAGATTGACAACAATGATGATCTTCATGACATATTTCTTGATCATCGTGTTAAAATCCTCATCAAGTGTTTGGTCCCTTTATCTGGGTGGAAACATTTGTTAGAATCAATGATACATTTACTTGATGATCCTGAGGAACAATATTCTTTGTATTTGAAAAAGAGAGTTGAAAAGCAGAAACAGAATGCACAAAAAAATGAGGAGGGTAATAAAAAACATAATGGAGATGAGGATTGTCCTTTGACATATGAGGAATTTGTAAACAATGAATTTGATTCAATCAGTCAGGAGCTGAAGATTTGTATGGTAGATTTGTACACCCACTTACCAACTTCTTGCATTTCACTTAAAGTGGTGAAGGGCATGGTCAGAGCTTTGGGTTTGCTCAAGTCTATTAAAACTTTATTGCATACTACTGGTGTTGCTAATGAAGGGTTGGAGTCAGTCTTCAAAGATTCCAAAGTTCCACGAAGCATTGTTGGTTCCCTTACGCAGTTGAGAACAAAGTGTACAAACACGCTAAAGTCCCTTCCTATAGAATTCTCTGTTCCTATTAATGAATATTCATTAAAGAACTTCTGCCTAGAAAATGCTTGCTTAATATTCTGTACTGCATCAACTTCTTCCAAATTGCATGATGTAACAGGAACAAGACCATTGGAATTGTTGGTCATTGATGAAGCTGCTCAGCTTAAAGAATGTGAATCAGCAATTCCTTTACAACTATCTGGTCTCCGCCATGCTATCCTTGTAGGAGATGAGAGGCAACTCCCTGCCATGGTTAAAAGCGAGATTGCAGTAAGTGCTGATTTTGGAAGAAGTTTGTTTGGAAGGCTGGCAAAGTTGGGGCACAAGAAGCACCTACTCAATGTCCAGTACAGGATGCATCCATCCATCAGTTTATTTCCAAAAAGGGAGTTCTACGACAACCAGATAGTAGATGGTCCAAATGTCAAAGAAAGAAGCTATGGGAGGTGCTGCCTCAAGGGAAAAATGTACCAATCCTATTCCTTCATAAATGTAGCCGATGGAAAAGAAGAATTTGATCACAGATTTAGTCGGAAAAATATGGTGGAGGTTGCTGCAGTCTCTGGGATAGTAGCAAGCCTTTATAAAGAATTTATTGGAACAAAGAAGAAAGTTAGTATTGGGGTCATATCACCATACAAGGCTCAAGTTTATGCAATTCAAGAGATACTCAGAAAATATACTGAAACTTCTGATACTGGATTCTCCGTAAGTGTGAGATCTGTTGATGGGTTCCAAGGTGGTGAAGAAGATGTGATAATTATATCTACTGTCCGATGTAATGGGAACGGGTCAGTTGGTTTCTTGTCAAACCAGCAAAGAGCAAATGTTGCCTTAACACGTGCAAGGTATTGCCTTTGGATATTGGGGAATGTGTCAACTTTGATTGCTAGTGACTCTGTTTGGAAGAAGCTAGTCCTTGATGCCAAGAAACGGAACTGTTTTTATAATGCTGATGAGGACAGTAACTTGGCTCAGGCTATTACAGCTGCCCTGTTGGTGCTTGACCAACTTCATTCTCTGCTTAATGTCGACTCTCTGCTGTTCAAAAATGCTATATGGAAGGTTTACCTCACTGATGACTTTTTGAACTCCATAACAAAAATTAAAGACACTGTGATTCTTTGGGAAGTGCTTGCGTTATTAACCAAGCTTTCGAGTGGATGGCGCCGACCTCTTAAGGATAAAGGAACTATAGTGTATGATGGGACTTCTGCTCAACTGTTAGAGAAGTATAAAATCAAAGGGAACTTGAATCTCATTTGGACTGTCGATATTCTCCAGGAGAATGCACATTACATCCAAGTTATGAAGTTTTGGGATATTTTGCCATTTTCTCATATACCAGTACTAGCCAAGCGTCTTGACATTGTTTTTGGGAATTTTACAGTGGACAAGATGAACCGCTGCCTTCACAAATGCATTGACAGGGATACTGTGGTTTTCAGCAATTACCCTATAGCTGATCATGAGGAGTTCCTTTCAAAACCATTGTCTTCTTTCAGTATAACAACTAATCGAGAAACAGCAACTTCAACATATGGGGAGACAGCAAAAGCGAAAAAAACAATTATCTCTTCAAAAAGCAATGTCAATGAAAAAGAAAGACGACTATGGAAAATAAAGGGTGAAGGAGTGGTGGATTTCAACACTTGCCTTGAAGCTAACCCTTCGGAGTTCTTGCCAAAACCATTATCATCACTAAGTCTAGCAGATAAGCCAGAAGCATCTACTTCCACTGACATGGAGACAGCGAAAGCAGTAAAACCAATTATCGCTTTGAAAAGCAATGTCAATCAAAAAGAAAAATTGTTATGGAAAATAAAGGGTGAAGGAGTGAAGGATTTCAGCACTTGCCTTGAAGCTAACCCTGTGGAGTTCTTGTCAAAACCATTATCATCACTAAGTCTAGCAGATAAGCCAGAAGCATCTACTTCCACTGACATGGAGACAGCGAAAGCAGTAAAACCAATTATCGCTTTGAAAAGCAATGTCAATCAAAAAGAAAAATTGTTATGGAAAATAAAGGGTGAAGAAGAAGTGAAGGATTTCAGCACTTGCGTTGAAGCTATCCCTGTGGAGTTCTTGCTCAAACCATTATCATCCCTAAGTCTAGCAGATAAGCCAGAAGCATCTACTTCCACTGACATGGAATCTGCGAAAAGATCCGCTACCAAAAGCGTTTCAAAAAGCACTGCGAGTCGAAGAAGGAGATCATCATCAAAACTAAAAGTCGAAGACCATGTGTTCGTTAAGATTCGACCTTGTTGTGGAATTAAATGGTATTCCCAAGGCGCAAAACTCACTCCACAGTTTACTTGTCCTTCTGAGGTTTCGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12

Protein Analysis

1307

Amino Acids

146.67

Weight (kDa)

8.62

Isoelectric Point (pI)

33.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6469 PF20073 80 - 212 3.4e-14 Domain of unknown function (DUF6469)
AAA_11 PF13086 254 - 615 1.4e-32 AAA domain
AAA_12 PF13087 623 - 819 6.1e-56 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000279)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65810 AT1G65810 AT1G65810 AT4G05540 AT5G37140 AT5G37150 AT5G37160 AT5G37165 AT5G52090
fragaria_vesca FvH4_1g27541 FvH4_1g27542 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_2g08960 FvH4_3g00170 FvH4_3g00170 FvH4_3g00170 FvH4_5g05930 FvH4_5g05930
malus_domestica MD08G1077700.v1.1 MD08G1077900.v1.1 MD08G1078000.v1.1 MD08G1078100.v1.1 MD08G1078300.v1.1 MD09G1057800.v1.1 MD09G1281400.v1.1 MD11G1222900.v1.1 MD14G1146400.v1.1
prunus_persica Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G201900_v2.0.a1 Prupe.1G202100_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.1G202500_v2.0.a1 Prupe.3G013100_v2.0.a1
pyrus_communis pycom09g18900 pycom09g18910 pycom13g26720 pycom13g26730 pycom13g26760 pycom13g26770
rosa_chinensis RchiOBHm_Chr3g0488001 RchiOBHm_Chr3g0488011 RchiOBHm_Chr3g0488031 RchiOBHm_Chr3g0488061 RchiOBHm_Chr3g0488111 RchiOBHm_Chr3g0488201 RchiOBHm_Chr3g0488211 RchiOBHm_Chr3g0488221 RchiOBHm_Chr3g0488321 RchiOBHm_Chr3g0488431 RchiOBHm_Chr6g0264601 RchiOBHm_Chr6g0264641
rosa_laevigata RLG00000014240 RLG00000014241 RLG00000022910 RLG00000022949 RLG00000030376
rosa_multiflora Rmu_co8075388.1_g000001 Rmu_co8424869.1_g000001 Rmu_co8427783.1_g000001 Rmu_sc0000546.1_g000002 Rmu_sc0000546.1_g000015 Rmu_sc0011052.1_g000014 Rmu_sc0017772.1_g000004
rosa_roxburghii Rroxscaffold_175G00431980 Rroxscaffold_175G00431990 Rroxscaffold_1G00034600 Rroxscaffold_6G00394230 Rroxscaffold_6G00394270 Rroxscaffold_6G00394280 Rroxscaffold_6G00394350 Rroxscaffold_7G00203240 Rroxscaffold_7G00203270 Rroxscaffold_7G00203280 Rroxscaffold_7G00203300 Rroxscaffold_7G00205740
rosa_rugosa Rorug03G0238000 Rorug03G0238100 Rorug03G0238100 Rorug03G0238500 Rorug05G0155400 Rorug05G0356000 Rorug05G0463000 Rorug06G0015200 Rorug06G0015300 Rorug06G0015400 Rorug06G0015500 Rorug06G0015500
rosa_samantha Rh1DG088100 Rh2AG244800 Rh2BG187500 Rh2CG247300 Rh3BG322700 Rh3BG322800 Rh3BG323100 Rh3BG323900 Rh3BG324000 Rh3BG324300 Rh3CG321300 Rh3CG321400 Rh3CG321500 Rh3DG319400 Rh3DG319900 Rh3DG320100 Rh3DG320200 Rh6AG133700 Rh6AG133800 Rh6AG133900 Rh6CG129300 Rh6CG129800 Rh6CG130200 Rh6DG116500 Rh6DG117200 Rh7DG378000
rosa_wichuraiana Rw2G014710 Rw3G025340 Rw3G025350 Rw3G025360 Rw5G020630 Rw6G011590 Rw6G011600 Rw7G041380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2166, 2529
Acc36I ACCTGC 1 cut(s) 996
AccB1I GGYRCC 1 cut(s) 2748
AccB7I CCANNNNNTGG 1 cut(s) 365
AccI GTMKAC 1 cut(s) 2613
AccIII TCCGGA 1 cut(s) 972
AciI CCGC 5 cut(s) 785, 947, 1789, 2986, 3750
AclWI GGATC 4 cut(s) 121, 134, 1175, 3741
AcsI RAATTY 8 cut(s) 476, 826, 1307, 1322, 1592, 2093, 2171, 2962
AcuI CTGAAG 1 cut(s) 1367
AcvI CACGTG 1 cut(s) 2423
AcyI GRCGYC 1 cut(s) 2749
AfaI GTAC 8 cut(s) 859, 1001, 1370, 1564, 1663, 1931, 2051, 2931
AfiI CCNNNNNNNGG 7 cut(s) 365, 860, 979, 1136, 1831, 2514, 3851
AflII CTTAAG 1 cut(s) 2759
AflIII ACRYGT 2 cut(s) 926, 2420
AhdI GACNNNNNGTC 1 cut(s) 879
AhlI ACTAGT 1 cut(s) 31
AjnI CCWGG 4 cut(s) 280, 704, 853, 2862
AleI CACNNNNGTG 1 cut(s) 140
AloI GAACNNNNNNTCC 2 cut(s) 1011, 1043
Alw21I GWGCWC 1 cut(s) 934
Alw26I GTCTC 5 cut(s) 1790, 2146, 3122, 3323, 3524
AlwI GGATC 4 cut(s) 121, 134, 1175, 3741
AlwNI CAGNNNCTG 3 cut(s) 326, 1345, 2144
Ama87I CYCGRG 1 cut(s) 995
Aor13HI TCCGGA 1 cut(s) 972
AoxI GGCC 2 cut(s) 422, 740
ApeKI GCWGC 8 cut(s) 48, 782, 962, 1736, 2031, 2135, 2570, 2988
ApoI RAATTY 8 cut(s) 476, 826, 1307, 1322, 1592, 2093, 2171, 2962
ArsI GACNNNNNNTTYG 6 cut(s) 2939, 2971, 3059, 3091, 3812, 3844
AseI ATTAAT 2 cut(s) 839, 1608
Asp700I GAANNNNTTC 3 cut(s) 167, 3425, 3629
AspLEI GCGC 2 cut(s) 2751, 3878
AspS9I GGNCC 4 cut(s) 344, 848, 1126, 1997
AsuHPI GGTGA 8 cut(s) 296, 812, 1420, 2205, 2336, 3221, 3422, 3623
AvaI CYCGRG 1 cut(s) 995
AvaII GGWCC 4 cut(s) 344, 848, 1126, 1997
AxyI CCTNAGG 1 cut(s) 1185
BaeGI GKGCMC 1 cut(s) 1905
BaeI ACNNNNGTAYC 2 cut(s) 3006, 3039
BamHI GGATCC 1 cut(s) 126
BanI GGYRCC 1 cut(s) 2748
BbrPI CACGTG 1 cut(s) 2423
BbsI GAAGAC 5 cut(s) 227, 878, 1492, 3069, 3825
Bbv12I GWGCWC 1 cut(s) 934
BbvI GCAGC 8 cut(s) 60, 769, 974, 1723, 2018, 2122, 2557, 2975
BccI CCATC 9 cut(s) 671, 825, 1951, 1955, 1988, 2075, 2303, 2739, 2780
BceAI ACGGC 1 cut(s) 1018
BciT130I CCWGG 4 cut(s) 282, 706, 855, 2864
BciVI GTATCC 1 cut(s) 3007
BclI TGATCA 3 cut(s) 1093, 2098, 3046
BcoDI GTCTC 5 cut(s) 1790, 2146, 3122, 3323, 3524
BcuI ACTAGT 1 cut(s) 31
BfmI CTRYAG 4 cut(s) 1587, 2136, 2775, 3039
BfoI RGCGCY 1 cut(s) 2752
BfrI CTTAAG 1 cut(s) 2759
BfuAI ACCTGC 1 cut(s) 996
BfuI GTATCC 1 cut(s) 3007
BglII AGATCT 1 cut(s) 2300
BisI GCNGC 8 cut(s) 49, 783, 963, 1737, 2032, 2136, 2571, 2989
BlpI GCTNAGC 1 cut(s) 1740
BlsI GCNGC 8 cut(s) 50, 784, 964, 1738, 2033, 2137, 2572, 2990
BmcAI AGTACT 1 cut(s) 2931
Bme1390I CCNGG 4 cut(s) 282, 706, 855, 2864
Bme18I GGWCC 4 cut(s) 344, 848, 1126, 1997
BmeRI GACNNNNNGTC 1 cut(s) 879
BmeT110I CYCGRG 1 cut(s) 995
BmgT120I GGNCC 4 cut(s) 344, 848, 1126, 1997
BmiI GGNNCC 6 cut(s) 128, 849, 1128, 1537, 2315, 2750
BmrFI CCNGG 4 cut(s) 282, 706, 855, 2864
BmrI ACTGGG 1 cut(s) 65
BmsI GCATC 7 cut(s) 1676, 1926, 1948, 2494, 3317, 3518, 3722
BmuI ACTGGG 1 cut(s) 65
BpiI GAAGAC 5 cut(s) 227, 878, 1492, 3069, 3825
BpmI CTGGAG 2 cut(s) 476, 2846
Bpu10I CCTNAGC 1 cut(s) 2556
Bpu1102I GCTNAGC 1 cut(s) 1740
BpuEI CTTGAG 4 cut(s) 206, 1424, 2021, 2211
BsaAI YACGTR 1 cut(s) 2423
BsaBI GATNNNNATC 2 cut(s) 333, 3799
BsaHI GRCGYC 1 cut(s) 2749
BsaI GGTCTC 1 cut(s) 1790
BsaJI CCNNGG 6 cut(s) 428, 704, 705, 1830, 2317, 3871
BsaWI WCCGGW 1 cut(s) 972
BsaXI ACNNNNNCTCC 6 cut(s) 916, 946, 1011, 1041, 3449, 3479
Bsc4I CCNNNNNNNGG 7 cut(s) 365, 860, 979, 1136, 1831, 2514, 3851
Bse1I ACTGG 9 cut(s) 60, 493, 727, 865, 988, 1474, 1927, 2284, 2927
Bse21I CCTNAGG 1 cut(s) 1185
Bse3DI GCAATG 4 cut(s) 375, 3175, 3376, 3577
Bse8I GATNNNNATC 2 cut(s) 333, 3799
BseAI TCCGGA 1 cut(s) 972
BseBI CCWGG 4 cut(s) 282, 706, 855, 2864
BseDI CCNNGG 6 cut(s) 428, 704, 705, 1830, 2317, 3871
BseGI GGATG 7 cut(s) 91, 1939, 1941, 1943, 2750, 2880, 3683
BseJI GATNNNNATC 2 cut(s) 333, 3799
BseLI CCNNNNNNNGG 7 cut(s) 365, 860, 979, 1136, 1831, 2514, 3851
BseMI GCAATG 4 cut(s) 375, 3175, 3376, 3577
BseMII CTCAG 6 cut(s) 639, 1176, 1754, 2266, 2570, 3900
BseNI ACTGG 9 cut(s) 60, 493, 727, 865, 988, 1474, 1927, 2284, 2927
BseRI GAGGAG 2 cut(s) 1268, 3068
BseSI GKGCMC 1 cut(s) 1905
BseXI GCAGC 8 cut(s) 60, 769, 974, 1723, 2018, 2122, 2557, 2975
BsgI GTGCAG 2 cut(s) 140, 934
BshFI GGCC 2 cut(s) 424, 742
BshNI GGYRCC 1 cut(s) 2748
BsiHKAI GWGCWC 1 cut(s) 934
BsiHKCI CYCGRG 1 cut(s) 995
BsiSI CCGG 1 cut(s) 973
BslFI GGGAC 3 cut(s) 1112, 1564, 2803
BslI CCNNNNNNNGG 7 cut(s) 365, 860, 979, 1136, 1831, 2514, 3851
BsmAI GTCTC 5 cut(s) 1790, 2146, 3122, 3323, 3524
BsmFI GGGAC 3 cut(s) 1112, 1564, 2803
BsmI GAATGC 2 cut(s) 1243, 2875
BsnI GGCC 2 cut(s) 424, 742
Bso31I GGTCTC 1 cut(s) 1790
BsoBI CYCGRG 1 cut(s) 995
Bsp1286I GDGCHC 2 cut(s) 934, 1905
Bsp13I TCCGGA 1 cut(s) 972
Bsp1407I TGTACA 2 cut(s) 1368, 1562
Bsp1720I GCTNAGC 1 cut(s) 1740
Bsp19I CCATGG 1 cut(s) 1830
BspACI CCGC 5 cut(s) 785, 947, 1789, 2986, 3750
BspANI GGCC 2 cut(s) 424, 742
BspCNI CTCAG 6 cut(s) 638, 1177, 1753, 2265, 2569, 3901
BspEI TCCGGA 1 cut(s) 972
BspHI TCATGA 2 cut(s) 1078, 3049
BspLI GGNNCC 6 cut(s) 128, 849, 1128, 1537, 2315, 2750
BspMAI CTGCAG 1 cut(s) 2140
BspMI ACCTGC 1 cut(s) 996
BspPI GGATC 4 cut(s) 121, 134, 1175, 3741
BspT107I GGYRCC 1 cut(s) 2748
BspTI CTTAAG 1 cut(s) 2759
BspTNI GGTCTC 1 cut(s) 1790
BsrDI GCAATG 4 cut(s) 375, 3175, 3376, 3577
BsrGI TGTACA 2 cut(s) 1368, 1562
BsrI ACTGG 9 cut(s) 60, 493, 727, 865, 988, 1474, 1927, 2284, 2927
BssECI CCNNGG 6 cut(s) 428, 704, 705, 1830, 2317, 3871
BssNI GRCGYC 1 cut(s) 2749
BssT1I CCWWGG 4 cut(s) 428, 1830, 2317, 3871
Bst2UI CCWGG 4 cut(s) 282, 706, 855, 2864
Bst6I CTCTTC 2 cut(s) 5, 3163
BstACI GRCGYC 1 cut(s) 2749
BstAFI CTTAAG 1 cut(s) 2759
BstAPI GCANNNNNTGC 2 cut(s) 1856, 2411
BstAUI TGTACA 2 cut(s) 1368, 1562
BstBAI YACGTR 1 cut(s) 2423
BstC8I GCNNGC 4 cut(s) 1648, 1890, 2158, 2718
BstDEI CTNAG 9 cut(s) 625, 1185, 1740, 2252, 2556, 3284, 3485, 3689, 3909
BstDSI CCRYGG 1 cut(s) 1830
BstF5I GGATG 7 cut(s) 91, 1939, 1941, 1943, 2750, 2880, 3683
BstH2I RGCGCY 1 cut(s) 2752
BstHHI GCGC 2 cut(s) 2751, 3878
BstMAI GTCTC 5 cut(s) 1790, 2146, 3122, 3323, 3524
BstMWI GCNNNNNNNGC 4 cut(s) 1856, 1909, 2411, 2567
BstNI CCWGG 4 cut(s) 282, 706, 855, 2864
BstNSI RCATGY 2 cut(s) 236, 930
BstSCI CCNGG 4 cut(s) 280, 704, 853, 2862
BstSFI CTRYAG 4 cut(s) 1587, 2136, 2775, 3039
BstSLI GKGCMC 1 cut(s) 1905
BstV1I GCAGC 8 cut(s) 60, 769, 974, 1723, 2018, 2122, 2557, 2975
BstV2I GAAGAC 5 cut(s) 227, 878, 1492, 3069, 3825
BstX2I RGATCY 3 cut(s) 126, 2300, 3746
BstXI CCANNNNNNTGG 3 cut(s) 3325, 3526, 3730
BstYI RGATCY 3 cut(s) 126, 2300, 3746
Bsu36I CCTNAGG 1 cut(s) 1185
BsuI GTATCC 1 cut(s) 3007
BsuRI GGCC 2 cut(s) 424, 742
BtgI CCRYGG 1 cut(s) 1830
BtsCI GGATG 7 cut(s) 91, 1939, 1941, 1943, 2750, 2880, 3683
BtsI GCAGTG 1 cut(s) 3774
BtsIMutI CAGTG 9 cut(s) 720, 808, 2655, 2694, 2976, 3318, 3519, 3723, 3774
BveI ACCTGC 1 cut(s) 996
Cac8I GCNNGC 4 cut(s) 1648, 1890, 2158, 2718
CaiI CAGNNNCTG 3 cut(s) 326, 1345, 2144
CciI TCATGA 2 cut(s) 1078, 3049
CfoI GCGC 2 cut(s) 2751, 3878
Cfr13I GGNCC 4 cut(s) 344, 848, 1126, 1997
CseI GACGC 1 cut(s) 2930
Csp6I GTAC 8 cut(s) 858, 1000, 1369, 1563, 1662, 1930, 2050, 2930
CspCI CAANNNNNGTGG 2 cut(s) 925, 960
CviQI GTAC 8 cut(s) 858, 1000, 1369, 1563, 1662, 1930, 2050, 2930
DdeI CTNAG 9 cut(s) 625, 1185, 1740, 2252, 2556, 3284, 3485, 3689, 3909
DinI GGCGCC 1 cut(s) 2750
DraI TTTAAA 1 cut(s) 909
DriI GACNNNNNGTC 1 cut(s) 879
Eam1104I CTCTTC 2 cut(s) 5, 3163
Eam1105I GACNNNNNGTC 1 cut(s) 879
EarI CTCTTC 2 cut(s) 5, 3163
EciI GGCGGA 1 cut(s) 1778
Eco130I CCWWGG 4 cut(s) 428, 1830, 2317, 3871
Eco147I AGGCCT 1 cut(s) 424
Eco31I GGTCTC 1 cut(s) 1790
Eco32I GATATC 1 cut(s) 373
Eco47I GGWCC 4 cut(s) 344, 848, 1126, 1997
Eco57I CTGAAG 1 cut(s) 1367
Eco72I CACGTG 1 cut(s) 2423
Eco81I CCTNAGG 1 cut(s) 1185
Eco88I CYCGRG 1 cut(s) 995
EcoO109I RGGNCCY 1 cut(s) 848
EcoRI GAATTC 2 cut(s) 826, 1592
EcoRII CCWGG 4 cut(s) 280, 704, 853, 2862
EcoRV GATATC 1 cut(s) 373
EcoT14I CCWWGG 4 cut(s) 428, 1830, 2317, 3871
EcoT22I ATGCAT 2 cut(s) 1941, 3005
EgeI GGCGCC 1 cut(s) 2750
EheI GGCGCC 1 cut(s) 2750
ErhI CCWWGG 4 cut(s) 428, 1830, 2317, 3871
FaqI GGGAC 3 cut(s) 1112, 1564, 2803
FauNDI CATATG 2 cut(s) 1300, 3121
FbaI TGATCA 3 cut(s) 1093, 2098, 3046
FblI GTMKAC 1 cut(s) 2613
Fnu4HI GCNGC 8 cut(s) 49, 783, 963, 1737, 2032, 2136, 2571, 2989
FokI GGATG 7 cut(s) 98, 1926, 1930, 1948, 2757, 2867, 3670
Fsp4HI GCNGC 8 cut(s) 49, 783, 963, 1737, 2032, 2136, 2571, 2989
GlaI GCGC 2 cut(s) 2750, 3877
GluI GCNGC 8 cut(s) 49, 783, 963, 1737, 2032, 2136, 2571, 2989
GsuI CTGGAG 2 cut(s) 476, 2846
HaeII RGCGCY 1 cut(s) 2752
HaeIII GGCC 2 cut(s) 424, 742
HapII CCGG 1 cut(s) 973
HgaI GACGC 1 cut(s) 2930
HhaI GCGC 2 cut(s) 2751, 3878
Hin1I GRCGYC 1 cut(s) 2749
Hin6I GCGC 2 cut(s) 2749, 3876
HinP1I GCGC 2 cut(s) 2749, 3876
HincII GTYRAC 3 cut(s) 985, 2460, 2614
HindII GTYRAC 3 cut(s) 985, 2460, 2614
HindIII AAGCTT 1 cut(s) 2732
HpaI GTTAAC 1 cut(s) 985
HpaII CCGG 1 cut(s) 973
HphI GGTGA 8 cut(s) 296, 812, 1420, 2205, 2336, 3221, 3422, 3623
Hpy166II GTNNAC 9 cut(s) 985, 1315, 1371, 1563, 2460, 2614, 2650, 2974, 3897
Hpy8I GTNNAC 9 cut(s) 985, 1315, 1371, 1563, 2460, 2614, 2650, 2974, 3897
HpyCH4IV ACGT 1 cut(s) 2422
HpyF10VI GCNNNNNNNGC 4 cut(s) 1856, 1909, 2411, 2567
HpyF3I CTNAG 9 cut(s) 625, 1185, 1740, 2252, 2556, 3284, 3485, 3689, 3909
HpySE526I ACGT 1 cut(s) 2422
Hsp92I GRCGYC 1 cut(s) 2749
HspAI GCGC 2 cut(s) 2749, 3876
KasI GGCGCC 1 cut(s) 2748
Kpn2I TCCGGA 1 cut(s) 972
Ksp22I TGATCA 3 cut(s) 1093, 2098, 3046
KspAI GTTAAC 1 cut(s) 985
LmnI GCTCC 2 cut(s) 937, 1342
Lsp1109I GCAGC 8 cut(s) 60, 769, 974, 1723, 2018, 2122, 2557, 2975
LweI GCATC 7 cut(s) 1676, 1926, 1948, 2494, 3317, 3518, 3722
MaeII ACGT 1 cut(s) 2422
MflI RGATCY 3 cut(s) 126, 2300, 3746
MhlI GDGCHC 2 cut(s) 934, 1905
Mly113I GGCGCC 1 cut(s) 2749
MlyI GAGTC 7 cut(s) 217, 889, 963, 1502, 2471, 2609, 3790
MmeI TCCRAC 3 cut(s) 346, 1470, 2093
Mph1103I ATGCAT 2 cut(s) 1941, 3005
MroI TCCGGA 1 cut(s) 972
MroXI GAANNNNTTC 3 cut(s) 167, 3425, 3629
MslI CAYNNNNRTG 4 cut(s) 140, 3323, 3524, 3728
MspA1I CMGCKG 2 cut(s) 2570, 2988
MspCI CTTAAG 1 cut(s) 2759
MspI CCGG 1 cut(s) 973
MspR9I CCNGG 4 cut(s) 282, 706, 855, 2864
Mva1269I GAATGC 2 cut(s) 1243, 2875
MvaI CCWGG 4 cut(s) 282, 706, 855, 2864
MwoI GCNNNNNNNGC 4 cut(s) 1856, 1909, 2411, 2567
NarI GGCGCC 1 cut(s) 2749
NcoI CCATGG 1 cut(s) 1830
NdeI CATATG 2 cut(s) 1300, 3121
NlaIV GGNNCC 6 cut(s) 128, 849, 1128, 1537, 2315, 2750
NmuCI GTSAC 5 cut(s) 142, 228, 284, 990, 2474
NsiI ATGCAT 2 cut(s) 1941, 3005
NspI RCATGY 2 cut(s) 236, 930
OliI CACNNNNGTG 1 cut(s) 140
PaeR7I CTCGAG 1 cut(s) 995
PagI TCATGA 2 cut(s) 1078, 3049
PasI CCCWGGG 1 cut(s) 705
PceI AGGCCT 1 cut(s) 424
PciI ACATGT 1 cut(s) 926
PctI GAATGC 2 cut(s) 1243, 2875
PdmI GAANNNNTTC 3 cut(s) 167, 3425, 3629
PflFI GACNNNGTC 1 cut(s) 226
PflMI CCANNNNNTGG 1 cut(s) 365
PfoI TCCNGGA 1 cut(s) 2862
PkrI GCNGC 8 cut(s) 50, 784, 964, 1738, 2033, 2137, 2572, 2990
PleI GAGTC 7 cut(s) 217, 888, 963, 1501, 2471, 2609, 3789
PluTI GGCGCC 1 cut(s) 2752
PmaCI CACGTG 1 cut(s) 2423
PmlI CACGTG 1 cut(s) 2423
PpsI GAGTC 7 cut(s) 217, 888, 963, 1501, 2471, 2609, 3789
Ppu21I YACGTR 1 cut(s) 2423
PpuMI RGGWCCY 1 cut(s) 848
PscI ACATGT 1 cut(s) 926
PshBI ATTAAT 2 cut(s) 839, 1608
PsiI TTATAA 2 cut(s) 2166, 2529
Psp5II RGGWCCY 1 cut(s) 848
Psp6I CCWGG 4 cut(s) 280, 704, 853, 2862
PspCI CACGTG 1 cut(s) 2423
PspGI CCWGG 4 cut(s) 280, 704, 853, 2862
PspN4I GGNNCC 6 cut(s) 128, 849, 1128, 1537, 2315, 2750
PspPI GGNCC 4 cut(s) 344, 848, 1126, 1997
PspPPI RGGWCCY 1 cut(s) 848
PspXI VCTCGAGB 1 cut(s) 995
PsrI GAACNNNNNNTAC 2 cut(s) 1546, 1578
PstI CTGCAG 1 cut(s) 2140
PstNI CAGNNNCTG 3 cut(s) 326, 1345, 2144
PsuI RGATCY 3 cut(s) 126, 2300, 3746
PsyI GACNNNGTC 1 cut(s) 226
PvuII CAGCTG 1 cut(s) 2570
RsaI GTAC 8 cut(s) 859, 1001, 1370, 1564, 1663, 1931, 2051, 2931
RsaNI GTAC 8 cut(s) 858, 1000, 1369, 1563, 1662, 1930, 2050, 2930
RseI CAYNNNNRTG 4 cut(s) 140, 3323, 3524, 3728
SalI GTCGAC 1 cut(s) 2612
SatI GCNGC 8 cut(s) 49, 783, 963, 1737, 2032, 2136, 2571, 2989
Sau96I GGNCC 4 cut(s) 344, 848, 1126, 1997
ScaI AGTACT 1 cut(s) 2931
SchI GAGTC 7 cut(s) 217, 889, 963, 1502, 2471, 2609, 3790
ScrFI CCNGG 4 cut(s) 282, 706, 855, 2864
SduI GDGCHC 2 cut(s) 934, 1905
SfaNI GCATC 7 cut(s) 1676, 1926, 1948, 2494, 3317, 3518, 3722
SfcI CTRYAG 4 cut(s) 1587, 2136, 2775, 3039
SfoI GGCGCC 1 cut(s) 2750
Sfr274I CTCGAG 1 cut(s) 995
SinI GGWCC 4 cut(s) 344, 848, 1126, 1997
SlaI CTCGAG 1 cut(s) 995
SmiMI CAYNNNNRTG 4 cut(s) 140, 3323, 3524, 3728
SmlI CTYRAG 6 cut(s) 185, 995, 1439, 2036, 2226, 2759
SmoI CTYRAG 6 cut(s) 185, 995, 1439, 2036, 2226, 2759
SpeI ACTAGT 1 cut(s) 31
SseBI AGGCCT 1 cut(s) 424
SsiI CCGC 5 cut(s) 785, 947, 1789, 2986, 3750
SspDI GGCGCC 1 cut(s) 2748
SspI AATATT 4 cut(s) 943, 1196, 1616, 1656
StuI AGGCCT 1 cut(s) 424
StyD4I CCNGG 4 cut(s) 280, 704, 853, 2862
StyI CCWWGG 4 cut(s) 428, 1830, 2317, 3871
TaiI ACGT 1 cut(s) 2425
TaqII GACCGA 1 cut(s) 361
TatI WGTACW 5 cut(s) 1368, 1562, 1661, 1929, 2929
TscAI CASTG 9 cut(s) 727, 808, 2662, 2701, 2976, 3325, 3526, 3730, 3781
TseFI GTSAC 5 cut(s) 142, 228, 284, 990, 2474
TseI GCWGC 8 cut(s) 48, 782, 962, 1736, 2031, 2135, 2570, 2988
Tsp45I GTSAC 5 cut(s) 142, 228, 284, 990, 2474
TspGWI ACGGA 3 cut(s) 593, 2278, 2530
TspRI CASTG 9 cut(s) 727, 808, 2662, 2701, 2976, 3325, 3526, 3730, 3781
Tth111I GACNNNGTC 1 cut(s) 226
Van91I CCANNNNNTGG 1 cut(s) 365
Vha464I CTTAAG 1 cut(s) 2759
VpaK11BI GGWCC 4 cut(s) 344, 848, 1126, 1997
VspI ATTAAT 2 cut(s) 839, 1608
XapI RAATTY 8 cut(s) 476, 826, 1307, 1322, 1592, 2093, 2171, 2962
XceI RCATGY 2 cut(s) 236, 930
XcmI CCANNNNNNNNNTGG 1 cut(s) 1991
XhoI CTCGAG 1 cut(s) 995
XmiI GTMKAC 1 cut(s) 2613
XmnI GAANNNNTTC 3 cut(s) 167, 3425, 3629
ZrmI AGTACT 1 cut(s) 2931
Zsp2I ATGCAT 2 cut(s) 1941, 3005
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.