AAA domain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 513, 1496 |
| AccB1I | GGYRCC | 1 cut(s) | 1195 |
| AccII | CGCG | 3 cut(s) | 1530, 1937, 2181 |
| AciI | CCGC | 4 cut(s) | 1158, 1530, 2181, 2225 |
| AclWI | GGATC | 3 cut(s) | 1476, 1489, 2021 |
| AcoI | YGGCCR | 1 cut(s) | 188 |
| AcsI | RAATTY | 7 cut(s) | 58, 271, 904, 1120, 1520, 1598, 2132 |
| AcvI | CACGTG | 1 cut(s) | 1853 |
| AdeI | CACNNNGTG | 2 cut(s) | 125, 1076 |
| AfaI | GTAC | 8 cut(s) | 139, 641, 650, 911, 1060, 1156, 1358, 1478 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 305, 581, 1135, 1321, 1649 |
| AflII | CTTAAG | 1 cut(s) | 980 |
| AflIII | ACRYGT | 2 cut(s) | 371, 1850 |
| AgsI | TTSAA | 7 cut(s) | 58, 170, 355, 889, 1043, 1374, 1670 |
| AjnI | CCWGG | 5 cut(s) | 298, 1128, 1200, 1270, 2246 |
| Alw21I | GWGCWC | 2 cut(s) | 885, 2040 |
| Alw26I | GTCTC | 1 cut(s) | 441 |
| AlwI | GGATC | 3 cut(s) | 1476, 1489, 2021 |
| AoxI | GGCC | 2 cut(s) | 188, 292 |
| ApaI | GGGCCC | 1 cut(s) | 296 |
| ApeKI | GCWGC | 6 cut(s) | 804, 1226, 1997, 2000, 2219, 2222 |
| ApoI | RAATTY | 7 cut(s) | 58, 271, 904, 1120, 1520, 1598, 2132 |
| AspLEI | GCGC | 2 cut(s) | 2118, 2181 |
| AspS9I | GGNCC | 4 cut(s) | 292, 293, 1424, 2190 |
| AsuHPI | GGTGA | 6 cut(s) | 77, 500, 890, 961, 1632, 1766 |
| AsuII | TTCGAA | 1 cut(s) | 1097 |
| AvaII | GGWCC | 2 cut(s) | 1424, 2190 |
| BaeGI | GKGCMC | 3 cut(s) | 296, 379, 1200 |
| BalI | TGGCCA | 1 cut(s) | 190 |
| BamHI | GGATCC | 1 cut(s) | 1481 |
| BanI | GGYRCC | 1 cut(s) | 1195 |
| BanII | GRGCYC | 3 cut(s) | 296, 1805, 2040 |
| BauI | CACGAG | 1 cut(s) | 416 |
| BbrPI | CACGTG | 1 cut(s) | 1853 |
| BbsI | GAAGAC | 2 cut(s) | 323, 1268 |
| Bbv12I | GWGCWC | 2 cut(s) | 885, 2040 |
| BbvCI | CCTCAGC | 1 cut(s) | 1554 |
| BbvI | GCAGC | 6 cut(s) | 816, 1213, 1984, 2012, 2209, 2231 |
| BccI | CCATC | 4 cut(s) | 284, 579, 1415, 1733 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1989, 2023, 2024, 2058 |
| BciT130I | CCWGG | 5 cut(s) | 300, 1130, 1202, 1272, 2248 |
| BciVI | GTATCC | 1 cut(s) | 1866 |
| BclI | TGATCA | 1 cut(s) | 960 |
| BcoDI | GTCTC | 1 cut(s) | 441 |
| BfaI | CTAG | 1 cut(s) | 1584 |
| BfmI | CTRYAG | 3 cut(s) | 228, 1317, 1998 |
| BfrI | CTTAAG | 1 cut(s) | 980 |
| BfuI | GTATCC | 1 cut(s) | 1866 |
| BisI | GCNGC | 6 cut(s) | 805, 1227, 1998, 2001, 2220, 2223 |
| BlpI | GCTNAGC | 2 cut(s) | 1230, 2240 |
| BlsI | GCNGC | 6 cut(s) | 806, 1228, 1999, 2002, 2221, 2224 |
| BmcAI | AGTACT | 1 cut(s) | 641 |
| Bme1390I | CCNGG | 5 cut(s) | 300, 1130, 1202, 1272, 2248 |
| Bme18I | GGWCC | 2 cut(s) | 1424, 2190 |
| BmgT120I | GGNCC | 4 cut(s) | 292, 293, 1424, 2190 |
| BmiI | GGNNCC | 4 cut(s) | 293, 294, 1197, 1483 |
| BmrFI | CCNGG | 5 cut(s) | 300, 1130, 1202, 1272, 2248 |
| BmrI | ACTGGG | 3 cut(s) | 105, 315, 1886 |
| BmsI | GCATC | 5 cut(s) | 336, 1093, 1169, 1353, 1375 |
| BmuI | ACTGGG | 3 cut(s) | 105, 315, 1886 |
| BpiI | GAAGAC | 2 cut(s) | 323, 1268 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 626, 658 |
| BpmI | CTGGAG | 1 cut(s) | 2230 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1554, 1986 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 1230, 2240 |
| Bpu14I | TTCGAA | 1 cut(s) | 1097 |
| BpuEI | CTTGAG | 5 cut(s) | 461, 641, 761, 962, 1049 |
| BsaAI | YACGTR | 1 cut(s) | 1853 |
| BsaJI | CCNNGG | 4 cut(s) | 299, 1200, 1462, 1747 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 121, 151, 2080, 2110 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 305, 581, 1135, 1321, 1649 |
| Bse1I | ACTGG | 6 cut(s) | 111, 310, 433, 880, 1354, 1881 |
| Bse3DI | GCAATG | 1 cut(s) | 2299 |
| BseBI | CCWGG | 5 cut(s) | 300, 1130, 1202, 1272, 2248 |
| BseDI | CCNNGG | 4 cut(s) | 299, 1200, 1462, 1747 |
| BseGI | GGATG | 5 cut(s) | 590, 778, 1366, 1368, 1978 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 305, 581, 1135, 1321, 1649 |
| BseMI | GCAATG | 1 cut(s) | 2299 |
| BseMII | CTCAG | 5 cut(s) | 1244, 1545, 1722, 2000, 2077 |
| BseNI | ACTGG | 6 cut(s) | 111, 310, 433, 880, 1354, 1881 |
| BseRI | GAGGAG | 2 cut(s) | 647, 860 |
| BseSI | GKGCMC | 3 cut(s) | 296, 379, 1200 |
| BseXI | GCAGC | 6 cut(s) | 816, 1213, 1984, 2012, 2209, 2231 |
| BsgI | GTGCAG | 2 cut(s) | 379, 2233 |
| Bsh1236I | CGCG | 3 cut(s) | 1530, 1937, 2181 |
| BshFI | GGCC | 2 cut(s) | 190, 294 |
| BshNI | GGYRCC | 1 cut(s) | 1195 |
| BsiHKAI | GWGCWC | 2 cut(s) | 885, 2040 |
| BslFI | GGGAC | 2 cut(s) | 316, 479 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 305, 581, 1135, 1321, 1649 |
| BsmAI | GTCTC | 1 cut(s) | 441 |
| BsmFI | GGGAC | 2 cut(s) | 316, 479 |
| BsnI | GGCC | 2 cut(s) | 190, 294 |
| Bsp119I | TTCGAA | 1 cut(s) | 1097 |
| Bsp120I | GGGCCC | 1 cut(s) | 292 |
| Bsp1286I | GDGCHC | 6 cut(s) | 296, 379, 885, 1200, 1805, 2040 |
| Bsp1407I | TGTACA | 3 cut(s) | 137, 648, 909 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 1230, 2240 |
| BspACI | CCGC | 4 cut(s) | 1158, 1530, 2181, 2225 |
| BspANI | GGCC | 2 cut(s) | 190, 294 |
| BspCNI | CTCAG | 5 cut(s) | 1243, 1546, 1721, 1999, 2076 |
| BspFNI | CGCG | 3 cut(s) | 1530, 1937, 2181 |
| BspLI | GGNNCC | 4 cut(s) | 293, 294, 1197, 1483 |
| BspMAI | CTGCAG | 2 cut(s) | 1321, 2002 |
| BspPI | GGATC | 3 cut(s) | 1476, 1489, 2021 |
| BspT104I | TTCGAA | 1 cut(s) | 1097 |
| BspT107I | GGYRCC | 1 cut(s) | 1195 |
| BspTI | CTTAAG | 1 cut(s) | 980 |
| BsrDI | GCAATG | 1 cut(s) | 2299 |
| BsrGI | TGTACA | 3 cut(s) | 137, 648, 909 |
| BsrI | ACTGG | 6 cut(s) | 111, 310, 433, 880, 1354, 1881 |
| BssECI | CCNNGG | 4 cut(s) | 299, 1200, 1462, 1747 |
| BssSI | CACGAG | 1 cut(s) | 416 |
| BssT1I | CCWWGG | 2 cut(s) | 1462, 1747 |
| Bst2BI | CACGAG | 1 cut(s) | 416 |
| Bst2UI | CCWGG | 5 cut(s) | 300, 1130, 1202, 1272, 2248 |
| Bst4CI | ACNGT | 4 cut(s) | 322, 863, 1783, 1904 |
| Bst6I | CTCTTC | 1 cut(s) | 783 |
| BstAFI | CTTAAG | 1 cut(s) | 980 |
| BstAUI | TGTACA | 3 cut(s) | 137, 648, 909 |
| BstBAI | YACGTR | 1 cut(s) | 1853 |
| BstBI | TTCGAA | 1 cut(s) | 1097 |
| BstC8I | GCNNGC | 6 cut(s) | 6, 263, 1141, 1532, 1705, 1727 |
| BstDEI | CTNAG | 6 cut(s) | 1230, 1554, 1708, 1986, 2063, 2240 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 1133, 1647 |
| BstF5I | GGATG | 5 cut(s) | 590, 778, 1366, 1368, 1978 |
| BstFNI | CGCG | 3 cut(s) | 1530, 1937, 2181 |
| BstHHI | GCGC | 2 cut(s) | 2118, 2181 |
| BstMAI | GTCTC | 1 cut(s) | 441 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 676, 813, 1559, 1997 |
| BstNI | CCWGG | 5 cut(s) | 300, 1130, 1202, 1272, 2248 |
| BstNSI | RCATGY | 2 cut(s) | 375, 781 |
| BstSCI | CCNGG | 5 cut(s) | 298, 1128, 1200, 1270, 2246 |
| BstSFI | CTRYAG | 3 cut(s) | 228, 1317, 1998 |
| BstSLI | GKGCMC | 3 cut(s) | 296, 379, 1200 |
| BstUI | CGCG | 3 cut(s) | 1530, 1937, 2181 |
| BstV1I | GCAGC | 6 cut(s) | 816, 1213, 1984, 2012, 2209, 2231 |
| BstV2I | GAAGAC | 2 cut(s) | 323, 1268 |
| BstX2I | RGATCY | 1 cut(s) | 1481 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 198 |
| BstYI | RGATCY | 1 cut(s) | 1481 |
| BsuI | GTATCC | 1 cut(s) | 1866 |
| BsuRI | GGCC | 2 cut(s) | 190, 294 |
| BtgZI | GCGATG | 3 cut(s) | 729, 831, 2031 |
| BtsCI | GGATG | 5 cut(s) | 590, 778, 1366, 1368, 1978 |
| BtsI | GCAGTG | 1 cut(s) | 1326 |
| BtsIMutI | CAGTG | 4 cut(s) | 873, 1326, 1441, 1779 |
| Cac8I | GCNNGC | 6 cut(s) | 6, 263, 1141, 1532, 1705, 1727 |
| CfoI | GCGC | 2 cut(s) | 2118, 2181 |
| Cfr13I | GGNCC | 4 cut(s) | 292, 293, 1424, 2190 |
| CseI | GACGC | 2 cut(s) | 77, 1943 |
| CsiI | ACCWGGT | 1 cut(s) | 1270 |
| Csp6I | GTAC | 8 cut(s) | 138, 640, 649, 910, 1059, 1155, 1357, 1477 |
| CviQI | GTAC | 8 cut(s) | 138, 640, 649, 910, 1059, 1155, 1357, 1477 |
| DdeI | CTNAG | 6 cut(s) | 1230, 1554, 1708, 1986, 2063, 2240 |
| DraI | TTTAAA | 1 cut(s) | 237 |
| DraIII | CACNNNGTG | 2 cut(s) | 125, 1076 |
| EaeI | YGGCCR | 1 cut(s) | 188 |
| Eam1104I | CTCTTC | 1 cut(s) | 783 |
| EarI | CTCTTC | 1 cut(s) | 783 |
| Ecl136II | GAGCTC | 1 cut(s) | 2038 |
| Eco130I | CCWWGG | 2 cut(s) | 1462, 1747 |
| Eco24I | GRGCYC | 3 cut(s) | 296, 1805, 2040 |
| Eco47I | GGWCC | 2 cut(s) | 1424, 2190 |
| Eco53kI | GAGCTC | 1 cut(s) | 2038 |
| Eco72I | CACGTG | 1 cut(s) | 1853 |
| EcoICRI | GAGCTC | 1 cut(s) | 2038 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 1133, 1647 |
| EcoO109I | RGGNCCY | 2 cut(s) | 292, 293 |
| EcoRI | GAATTC | 2 cut(s) | 58, 1598 |
| EcoRII | CCWGG | 5 cut(s) | 298, 1128, 1200, 1270, 2246 |
| EcoT14I | CCWWGG | 2 cut(s) | 1462, 1747 |
| EcoT22I | ATGCAT | 2 cut(s) | 783, 1368 |
| EcoT38I | GRGCYC | 3 cut(s) | 296, 1805, 2040 |
| ErhI | CCWWGG | 2 cut(s) | 1462, 1747 |
| FaqI | GGGAC | 2 cut(s) | 316, 479 |
| FauI | CCCGC | 1 cut(s) | 1523 |
| FbaI | TGATCA | 1 cut(s) | 960 |
| Fnu4HI | GCNGC | 6 cut(s) | 805, 1227, 1998, 2001, 2220, 2223 |
| FokI | GGATG | 5 cut(s) | 597, 785, 1353, 1375, 1985 |
| FriOI | GRGCYC | 3 cut(s) | 296, 1805, 2040 |
| Fsp4HI | GCNGC | 6 cut(s) | 805, 1227, 1998, 2001, 2220, 2223 |
| FspBI | CTAG | 1 cut(s) | 1584 |
| GlaI | GCGC | 2 cut(s) | 2117, 2180 |
| GluI | GCNGC | 6 cut(s) | 805, 1227, 1998, 2001, 2220, 2223 |
| GsuI | CTGGAG | 1 cut(s) | 2230 |
| HaeIII | GGCC | 2 cut(s) | 190, 294 |
| HgaI | GACGC | 2 cut(s) | 77, 1943 |
| HhaI | GCGC | 2 cut(s) | 2118, 2181 |
| Hin6I | GCGC | 2 cut(s) | 2116, 2179 |
| HinP1I | GCGC | 2 cut(s) | 2116, 2179 |
| HincII | GTYRAC | 3 cut(s) | 430, 1251, 1829 |
| HindII | GTYRAC | 3 cut(s) | 430, 1251, 1829 |
| HpaI | GTTAAC | 1 cut(s) | 430 |
| HphI | GGTGA | 6 cut(s) | 77, 500, 890, 961, 1632, 1766 |
| Hpy166II | GTNNAC | 5 cut(s) | 430, 617, 912, 1251, 1829 |
| Hpy188III | TCNNGA | 8 cut(s) | 418, 440, 481, 536, 629, 1043, 1670, 1931 |
| Hpy8I | GTNNAC | 5 cut(s) | 430, 617, 912, 1251, 1829 |
| Hpy99I | CGWCG | 1 cut(s) | 2298 |
| HpyAV | CCTTC | 4 cut(s) | 748, 1352, 1380, 1612 |
| HpyCH4III | ACNGT | 4 cut(s) | 322, 863, 1783, 1904 |
| HpyCH4IV | ACGT | 4 cut(s) | 682, 839, 1852, 2309 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 676, 813, 1559, 1997 |
| HpyF3I | CTNAG | 6 cut(s) | 1230, 1554, 1708, 1986, 2063, 2240 |
| HpySE526I | ACGT | 4 cut(s) | 682, 839, 1852, 2309 |
| HspAI | GCGC | 2 cut(s) | 2116, 2179 |
| Ksp22I | TGATCA | 1 cut(s) | 960 |
| KspAI | GTTAAC | 1 cut(s) | 430 |
| LmnI | GCTCC | 2 cut(s) | 2043, 2249 |
| Lsp1109I | GCAGC | 6 cut(s) | 816, 1213, 1984, 2012, 2209, 2231 |
| LweI | GCATC | 5 cut(s) | 336, 1093, 1169, 1353, 1375 |
| MabI | ACCWGGT | 1 cut(s) | 1270 |
| MaeI | CTAG | 1 cut(s) | 1584 |
| MaeII | ACGT | 4 cut(s) | 682, 839, 1852, 2309 |
| MaeIII | GTNAC | 7 cut(s) | 65, 403, 1015, 1029, 1068, 1904, 2200 |
| MflI | RGATCY | 1 cut(s) | 1481 |
| MhlI | GDGCHC | 6 cut(s) | 296, 379, 885, 1200, 1805, 2040 |
| MlsI | TGGCCA | 1 cut(s) | 190 |
| MluNI | TGGCCA | 1 cut(s) | 190 |
| MlyI | GAGTC | 4 cut(s) | 85, 408, 1256, 1686 |
| MmeI | TCCRAC | 1 cut(s) | 2123 |
| Mox20I | TGGCCA | 1 cut(s) | 190 |
| Mph1103I | ATGCAT | 2 cut(s) | 783, 1368 |
| MscI | TGGCCA | 1 cut(s) | 190 |
| MslI | CAYNNNNRTG | 1 cut(s) | 370 |
| Msp20I | TGGCCA | 1 cut(s) | 190 |
| MspA1I | CMGCKG | 2 cut(s) | 2084, 2222 |
| MspCI | CTTAAG | 1 cut(s) | 980 |
| MspR9I | CCNGG | 5 cut(s) | 300, 1130, 1202, 1272, 2248 |
| MvaI | CCWGG | 5 cut(s) | 300, 1130, 1202, 1272, 2248 |
| MvnI | CGCG | 3 cut(s) | 1530, 1937, 2181 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 676, 813, 1559, 1997 |
| NlaIV | GGNNCC | 4 cut(s) | 293, 294, 1197, 1483 |
| NmuCI | GTSAC | 3 cut(s) | 65, 1068, 2200 |
| NsiI | ATGCAT | 2 cut(s) | 783, 1368 |
| NspI | RCATGY | 2 cut(s) | 375, 781 |
| NspV | TTCGAA | 1 cut(s) | 1097 |
| PciI | ACATGT | 1 cut(s) | 371 |
| PfeI | GAWTC | 7 cut(s) | 170, 215, 589, 625, 1743, 1812, 1886 |
| PfoI | TCCNGGA | 1 cut(s) | 2246 |
| PkrI | GCNGC | 6 cut(s) | 806, 1228, 1999, 2002, 2221, 2224 |
| PleI | GAGTC | 4 cut(s) | 85, 408, 1255, 1685 |
| PmaCI | CACGTG | 1 cut(s) | 1853 |
| PmlI | CACGTG | 1 cut(s) | 1853 |
| PpsI | GAGTC | 4 cut(s) | 85, 408, 1255, 1685 |
| Ppu21I | YACGTR | 1 cut(s) | 1853 |
| PscI | ACATGT | 1 cut(s) | 371 |
| PsiI | TTATAA | 2 cut(s) | 513, 1496 |
| Psp124BI | GAGCTC | 1 cut(s) | 2040 |
| Psp6I | CCWGG | 5 cut(s) | 298, 1128, 1200, 1270, 2246 |
| PspCI | CACGTG | 1 cut(s) | 1853 |
| PspGI | CCWGG | 5 cut(s) | 298, 1128, 1200, 1270, 2246 |
| PspN4I | GGNNCC | 4 cut(s) | 293, 294, 1197, 1483 |
| PspOMI | GGGCCC | 1 cut(s) | 292 |
| PspPI | GGNCC | 4 cut(s) | 292, 293, 1424, 2190 |
| PstI | CTGCAG | 2 cut(s) | 1321, 2002 |
| PsuI | RGATCY | 1 cut(s) | 1481 |
| PvuII | CAGCTG | 2 cut(s) | 2084, 2222 |
| RsaI | GTAC | 8 cut(s) | 139, 641, 650, 911, 1060, 1156, 1358, 1478 |
| RsaNI | GTAC | 8 cut(s) | 138, 640, 649, 910, 1059, 1155, 1357, 1477 |
| RseI | CAYNNNNRTG | 1 cut(s) | 370 |
| SacI | GAGCTC | 1 cut(s) | 2040 |
| SatI | GCNGC | 6 cut(s) | 805, 1227, 1998, 2001, 2220, 2223 |
| Sau96I | GGNCC | 4 cut(s) | 292, 293, 1424, 2190 |
| ScaI | AGTACT | 1 cut(s) | 641 |
| SchI | GAGTC | 4 cut(s) | 85, 408, 1256, 1686 |
| ScrFI | CCNGG | 5 cut(s) | 300, 1130, 1202, 1272, 2248 |
| SduI | GDGCHC | 6 cut(s) | 296, 379, 885, 1200, 1805, 2040 |
| SexAI | ACCWGGT | 1 cut(s) | 1270 |
| SfaNI | GCATC | 5 cut(s) | 336, 1093, 1169, 1353, 1375 |
| SfcI | CTRYAG | 3 cut(s) | 228, 1317, 1998 |
| SfuI | TTCGAA | 1 cut(s) | 1097 |
| SinI | GGWCC | 2 cut(s) | 1424, 2190 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 370 |
| SmlI | CTYRAG | 6 cut(s) | 440, 620, 740, 941, 980, 1064 |
| SmoI | CTYRAG | 6 cut(s) | 440, 620, 740, 941, 980, 1064 |
| SsiI | CCGC | 4 cut(s) | 1158, 1530, 2181, 2225 |
| SspI | AATATT | 2 cut(s) | 37, 1149 |
| SspMI | CTAG | 1 cut(s) | 1584 |
| SstI | GAGCTC | 1 cut(s) | 2040 |
| StyD4I | CCNGG | 5 cut(s) | 298, 1128, 1200, 1270, 2246 |
| StyI | CCWWGG | 2 cut(s) | 1462, 1747 |
| TaaI | ACNGT | 4 cut(s) | 322, 863, 1783, 1904 |
| TaiI | ACGT | 4 cut(s) | 685, 842, 1855, 2312 |
| TaqI | TCGA | 8 cut(s) | 179, 412, 843, 986, 1097, 1889, 2009, 2034 |
| TaqII | GACCGA | 1 cut(s) | 2168 |
| TatI | WGTACW | 5 cut(s) | 137, 639, 648, 909, 1356 |
| TfiI | GAWTC | 7 cut(s) | 170, 215, 589, 625, 1743, 1812, 1886 |
| TscAI | CASTG | 4 cut(s) | 880, 1326, 1441, 1786 |
| TseFI | GTSAC | 3 cut(s) | 65, 1068, 2200 |
| TseI | GCWGC | 6 cut(s) | 804, 1226, 1997, 2000, 2219, 2222 |
| Tsp45I | GTSAC | 3 cut(s) | 65, 1068, 2200 |
| TspDTI | ATGAA | 9 cut(s) | 35, 105, 123, 228, 512, 801, 1236, 2034, 2180 |
| TspGWI | ACGGA | 4 cut(s) | 1203, 1494, 1708, 2267 |
| TspRI | CASTG | 4 cut(s) | 880, 1326, 1441, 1786 |
| Vha464I | CTTAAG | 1 cut(s) | 980 |
| VpaK11BI | GGWCC | 2 cut(s) | 1424, 2190 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 1133, 1647 |
| XapI | RAATTY | 7 cut(s) | 58, 271, 904, 1120, 1520, 1598, 2132 |
| XceI | RCATGY | 2 cut(s) | 375, 781 |
| XspI | CTAG | 1 cut(s) | 1584 |
| ZrmI | AGTACT | 1 cut(s) | 641 |
| Zsp2I | ATGCAT | 2 cut(s) | 783, 1368 |